CN108120788A

CN108120788A - HPLC analysis methods on five Carotenoids of detection

Info

Publication number: CN108120788A
Application number: CN201611068609.8A
Authority: CN
Inventors: 康迎春
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-11-29
Filing date: 2016-11-29
Publication date: 2018-06-05

Abstract

5 kinds of major carotenoids neoxathins in Chinese wolfberry fruit, xanthophyll, luteole, β kryptoxanthins and the HPLC analytical method of β carrots are established, analysis measures 5 kinds of major carotenoids contents in 7 different cultivars Chinese wolfberry fruits.Sample is extracted through tetrahydrofuran, saponification 8h post analysis measure, and is separated using YMC C30 columns (4.6 × 250mm, 5 μm), mobile phase A phase：(0.1% triethylamine) methanol/water（75:25, V/V）, B phases：Acetone/methylene chloride/methanol（70:5:25）Gradient elution, flow velocity 1mL/min, 25 DEG C of column temperature detect at wavelength 450nm.The average recovery rate of 5 Carotenoids is 87.5%~91.5%, and minimum detectability is 0.0007~0.0062 μ g/ μ L.The accuracy of this method and precision are all very high, the quantitative analysis available for medlar fresh fruit carotenoid.

Description

HPLC analysis methods on five Carotenoids of detection

Technical field

The species of carotenoid is various, and standard items are expensive, and most of research is to analyze one or two Carotenoids, the method It can be to carotenoid in five（Neoxathin, xanthophyll, luteole, beta-cryptoxanthin and β-carrot）Analysis is carried out at the same time, than Compared with other methods, HPLC analysis methods have the characteristics that short detection time, high sensitivity, detected value are accurate, substantially increase inspection It surveys efficiency and detects the reliability of data.

Background technology

Establish 5 Carotenoids neoxathins, xanthophyll, luteole, beta-cryptoxanthin and the efficient liquid phase of β-carrot Chromatogram analysis method, this method high sensitivity, stability and reappearance are good, and the rate of recovery is higher, can be as carotenoid point The method application of analysis.

The content of the invention

HPLC analysis conditions：It is separated using YMC-C30 columns (4.6 × 250mm, 5 μm), mobile phase A phase：(0.1% 3 second Amine) methanol/water（75:25, V/V）, B phases：Acetone/methylene chloride/methanol（70:5:25, V/V/V）, gradient elution program： 0min, 100%A, 0% B；5min, 75%A, 25% B；10min, 50%A, 50% B；15min, 15%A, 85% B；35min, 0%A, 100% B；45min, 100%A, 0%B, flow velocity 1mL/min, 25 DEG C of column temperature, Detection wavelength 450nm.

Carotenoid is the fat-soluble pigment being present in plant and microorganism, most carotenoid in yellow, Orange or red.Numerous medical researches show that carotenoid plays an important role of efficient singlet-oxygen quenching and removes free radical, So as to reduce damage of the free radical to cellular genetic material and cell membrane.Epidemiological study shows carotenoid in prevention people Even more important effect is played in terms of the protection human health such as class angiocardiopathy and cancer-resisting.But human body itself cannot synthesize Carotenoid, fruits and vegetables are the important sources that the mankind obtain carotenoid.Matrimony vine is the functional characteristic of integration of drinking and medicinal herbs Plant resources rich in various bioactivators such as carotenoid, Flavonoid substances and polysaccharides, is widely used in protecting Health food industry.At present for the research of Catotenoids From Lycium Barbarum be concentrated mainly on different sources, maturity red fruit matrimony vine in The research of carotenoid content, the research in relation to carotenoid in yellow fruit and black fruit fructus lycii and hybridization matrimony vine strain rarely have report Road.Using HPLC analysis methods to it is several hybridization strain yellow fruits and black fruit fructus lycii fresh fruit in 5 kinds of major carotenoids components and Content carries out analysis measure, to understand the medlar fresh fruit carotenoid of different cultivars composition, is carried for its deep processing and utilization For foundation.

Claims

1. by this research, 5 kinds of major carotenoids neoxathins, xanthophyll, luteole, β-hidden in Chinese wolfberry fruit are established Yellow matter and the HPLC analytical method of β-carrot, this method high sensitivity, stability and reappearance are good, the rate of recovery Method application that is higher, being analyzed as medlar fresh fruit carotenoid.

2. the experimental results showed that saponification is the important step of carotenoid pre-treatment, determine that sample suitable for saponification time is 8h.

3. carotenoid is not only present in red fruit matrimony vine, and is present in yellow fruit, black fruit and hybridization strain matrimony vine, content Height is different due to kind.

4. present study provides theoretical ginseng for further further investigation different cultivars Chinese wolfberry fruit carotenoid composition and content It examines.