CN108107097A - A kind of polyacrylamide electroconductive molecule blotting membrane and its preparation method and application - Google Patents
A kind of polyacrylamide electroconductive molecule blotting membrane and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of polyacrylamide electroconductive molecule blotting membranes and its preparation method and application.The present invention makes acrylamide, template molecule and conductive component polymerize jointly in the original location first with electropolymerization mode, form polyacrylamide film, recycle the mode of electrochemistry elution except the template molecule in striping, so as to obtain high conductivity and there is the acrylamide polymer film of function of molecular engram.The preparation method of the present invention has the characteristics that in-situ polymerization, simplicity and quick.In addition, the conductive hydrogel molecular engram film can be applied to some specific moleculars, the detection of such as carmine molecule content because of its hydrophily, Low ESR and special function of molecular engram.The method that quantitative detection is carried out to carmine molecule using this molecular engram film has the advantages that highly sensitive and high reappearance.
Description
Technical field
The invention discloses a kind of polyacrylamide conductivity water molecular engram films and its preparation method and application, belong to membrane material
Material and electrochemical sensor technology field.
Background technology
Molecular imprinting technology is that one that developed recently gets up combines polymer chemistry, material science, chemical engineering and life
The cross discipline technology of object chemistry.It utilizes the phase interaction between molecularly imprinted polymer simulation enzyme-substrate or antibody-antigene
With being also referred to as template molecule to microsphere and carry out single-minded identification.Due to it this Selective recognition act on, absorption, separation,
The fields such as catalysis and target analyte detection are with a wide range of applications, so as to cause the very big concern of people.However conventional
Polymer band gap is very wide, is substantially not present electronics on antibonding orbital at room temperature, causes electric conductivity very low, belongs to insulator,
It is difficult to apply to Electrochemical Detection.In order to solve the problems, such as this, generally use doped graphene, nanogold and is received into polymer
The contour conductive constituent of rice silver.Although these methods can effectively improve the conductive capability of polymer, synthesis technology is cumbersome,
It is expensive, be unable to large-scale industrial production, so application value is not high.
The content of the invention
For overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of polyacrylamide electroconductive molecule traces
Film and its preparation method and application.Conductive hydrogel technology is combined by the present invention with molecular imprinting technology, synthesizes one kind i.e.
With function of molecular engram, and there is the polymer film of high conductivity.To overcome conductance in existing molecular engram membrane technology
The defects of rate is not high and selective low.The film, which also has, prepares the characteristics such as simple, operation is easy and of low cost.The present invention will
Above-mentioned polyacrylamide electroconductive molecule blotting membrane applies in the detection of carmine molecule, and detection kermes can be quantified by providing one kind
Red method, this method have the advantages that high sensitivity, high precision, high accuracy, high-recovery and high reappearance.
In the present invention, molecular engram film is the polymer film for having the characteristics that molecular engram and Low ESR two.Its preparation side
Method makes acrylamide, template molecule (famille rose) and conductive component (dopamine D A, phytic acid PA) in original using electropolymerization mode
The common polymerization in position, forms polyacrylamide film, recycles the mode of electrochemistry elution except the template molecule in striping, so as to obtain
High conductivity and the acrylamide polymer film with function of molecular engram.
Technical scheme is specifically described as follows.
A kind of preparation method of polyacrylamide electroconductive molecule blotting membrane, is as follows, is as follows:
(1) by acrylamide, N, N- methylene diacrylamides, potassium peroxydisulfate and potassium nitrate are mixed, stirred evenly in proportion
Afterwards, mixed liquor A is obtained;Template molecule, dopamine D A and phytic acid PA are mixed in proportion, after stirring evenly, obtain mixed liquid B;
After mixed liquor A and mixed liquid B are mixed polymer fluid is obtained after ultrasound condition pre-reaction;
(2) by glass carbon working electrode, saturation Ag/AgCl reference electrodes and platinum filament, to electrode, inserting step (1) obtains together
Polymer fluid in, three electrodes are connected by electrochemical workstation, using cyclic voltammetry carry out electroinitiated polymerization reaction, polymerization complete
Afterwards a layer polypropylene amide electroconductive molecule blotting membrane is formed in glass carbon working electrode surface;
(3) the glass carbon working electrode of the polyacrylamide electroconductive molecule blotting membrane of preparation is placed in deionized water and rinses 2-
3 times, to remove the unpolymerized component that may be adsorbed;Afterwards by the good glass carbon working electrode of above-mentioned rinsing, saturation Ag/AgCl references
Electrode and platinum filament are inserted into the sodium hydroxide solution of 0.05~0.15mol/L electrode together, and three electrodes are by electrochemical workstation
Connection;Differential pulse voltammetry carries out electrochemistry elution to template, obtains polyacrylamide electroconductive molecule blotting membrane.
In the present invention, in step (1), acrylamide AA, N, N- methylene diacrylamides MBA, potassium peroxydisulfate and potassium nitrate
Mass ratio be (7~8):(2~3):(2~3):(4:5).
In the present invention, in step (1), the mass ratio of template molecule, dopamine D A and phytic acid PA is (5~6):28:(1~
2)。
In the present invention, in step (1), template molecule is famille rose.
In the present invention, in step (1), the volume ratio of mixed liquor A and mixed liquid B is 1:1~1:1.5.
In the present invention, in step (2), during cyclic voltammetry, initial potential:- 0.4V terminates current potential:- 1.3V, highest electricity
Position:- 0.4V, potential minimum:- 1.3V, sweep speed:0.03V/s, cycle-index:25 times~35 times, the sampling interval:0.001V,
Sedimentation time:2s~3s, sensitivity:10-4。
In the present invention, in step (3), in differential pulse voltammetry, initial potential:0.0V terminates current potential:1.0V, increment
Voltage:0.004V, amplitude:0.05V, pulse width:0.2V, sampling width:0.0167s, pulse period:0.5s, sedimentation time:
2s, sensitivity:10-6。
The present invention also provides the polyacrylamide electroconductive molecule blotting membranes that a kind of above-mentioned preparation method obtains.Preferably,
A diameter of 1~the 3mm of polyacrylamide electroconductive molecule blotting membrane;Polyacrylamide electroconductive molecule trace membrane impedance is 45~55ohm.
The present invention further provides above-mentioned polyacrylamide electroconductive molecule blotting membrane answering in carmine content detection
With application process is as follows:
(1), carmine red standard solution and the preparation to be measured containing carmine ingredient juice solution
Compound concentration is respectively the carmine red standard solution of 2mg/L, 4mg/L, 6mg/L, 8mg/L, 10mg/L, 12mg/L,
The solution 10mL to be measured containing carmine ingredient is taken, 50mL is settled to deionized water;
(2), the quantitative detection of carmine molecule
By the glass carbon working electrode difference inserting step (1) of the conductive carmine molecular engram film of seven attachment polyacrylamides
Prepare each solution in, stir 20~40min, fully absorption after, be placed in deionized water and rinse 2-3 times, after successively will be above-mentioned
Seven glass carbon working electrodes, saturation Ag/AgCl reference electrodes and the platinum filament rinsed is inserted into substance withdrawl syndrome together to electrode
In hac buffer for the pH=4 of 0.1mol/L, three electrodes are connected by electrochemical workstation;Pass through differential pulse voltammetry
It is measured, is successively read the peak current corresponding to above-mentioned seven glass carbon working electrode respectively;Differential pulse voltammetry it is specific
Condition is as follows:Initial voltage:0.0V, end voltage:1.0V, incremental voltage:0.004V, amplitude:0.05V, pulse width:
0.2V, sampling width:0.0167s, pulse period:0.5s, sedimentation time:2s, sensitivity:10-6;
(3), the drafting of standard curve
Using peak current as ordinate, carmine concentration corresponding to carmine red standard solution is abscissa, draw peak current with
The curve between carmine concentration corresponding to carmine red standard solution, fitting obtains carmine concentration and the standard of peak current is bent
Line equation;
(4), measure containing content carmine in carmine ingredient juice solution
The peak current of the solution to be measured containing carmine ingredient measured in step (2) is substituted into kermes obtained by step (3)
In red concentration and the calibration curve equation of peak current, concentration carmine in solution to be measured is calculated, then according to step (1)
In extension rate, so as to which content carmine in solution to be measured be obtained.
Compared to the prior art, the beneficial effects of the present invention are:
The present invention not only overcomes and graphite is used in prior art by adulterating phytic acid and dopamine into acrylamide
The shortcomings that synthesis is expensive caused by the inorganic highly conductive component such as alkene, nanogold and nano silver is also used phytic acid, DOPA
The method that amine and acrylamide polymerize jointly for the conductive polymer membrane reported in the past, simplifies synthesis step, just
In progress large-scale industrial production.
Further, the present invention also provides a kind of method detected using above-mentioned film to template molecule famille rose molecular method quantification,
It is linear good especially in the range of 2mg/L~12mg/L, linearly dependent coefficient R2=0.984.Therefore this method has very high
Sensitivity, precision, accuracy and the rate of recovery, and detection time is short, and service life is long, favorable reproducibility.
Further, the present invention prepares electroconductive molecule trace using some other specific template molecules beyond famille rose
During film, the molecular engram film of acquisition can carry out specific template molecule quantitative detection.
Description of the drawings
Fig. 1, blank polyacrylamide film surface scanning electron microscope phenogram.
Fig. 2, polyacrylamide electroconductive molecule blotting membrane surface scan Electronic Speculum phenogram.
Fig. 3, glass-carbon electrode, blank polyacrylamide membrane electrode, polyacrylamide conductive traces film are in potassium ferricyanide solution
Middle detection cyclic voltammetry curve figure.
Fig. 4, glass-carbon electrode, blank polyacrylamide membrane electrode, polyacrylamide conductive traces film are in potassium ferricyanide solution
Middle detection electrochemical impedance graph.
Fig. 5, polyacrylamide electroconductive molecule blotting membrane principle schematic is prepared.
Fig. 6, the polyacrylamide electroconductive molecule blotting membrane electropolymerization installation drawing i.e. microstructure of membrane electrode, wherein 1- are prepared
Glass carbon working electrode, 2- platinum filaments are to electrode, 3-Ag/AgCl reference electrodes, 4- filamentary silvers, 5- saturated potassium chloride solutions, 6- polymer fluids,
7- beakers;A represents polyacrylamide electroconductive molecule blotting membrane obtained by polymerization in illustration, and B represents glass carbon working electrode.
Fig. 7, the polyacrylamide electroconductive molecule print for being adsorbed with various various concentration carmine red standard solution in example 2
Differential pulse voltammetry responds picture on mark membrane electrode.
Fig. 8, the polyacrylamide electroconductive molecule blotting membrane containing carmine ingredient juice solution is adsorbed in example 2
Differential pulse voltammetry responds picture on electrode.
Curve between Fig. 9, carmine red standard solution peak current carmine concentration corresponding with standard solution.
It is Figure 10, opposite with carmine red standard solution peak current and standard solution containing carmine ingredient juice solution peak current
Answer the standard curve between carmine concentration.
Specific embodiment
The present invention is further illustrated with reference to embodiment, but is not intended to limit the present invention.
Embodiment 1
For the preparation of the conductive carmine molecular engram film of polyacrylamide of carmine molecular method quantification detection
By raw material propylene amide, N, N- methylene diacrylamides, potassium peroxydisulfate and 0.2mol/L KNO3According to 780mg:
220mg:220mg:The ratio mixing of 10mL, after being slowly stirred uniformly, forms mixed liquor A;By raw material is carmine, dopamine and
70% phytic acid is according to 51mg:280mg:The mixing that feeds intake of 10mL after being slowly stirred uniformly, forms mixed liquid B;By mixed liquor A and B
It mixes and the pre-reaction 1h under ultrasound condition, after being cooled to room temperature 25 DEG C, then nitrogen 10min is passed through into solution, and with fresh-keeping
Film seals.Obtain ready polymer fluid;
Glass carbon working electrode, saturation Ag/AgCl reference electrodes and platinum filament are inserted into above-mentioned polymer fluid electrode together, three
Electrode is connected by electrochemical workstation;It is initial potential in each technical parameter with the function of cyclic voltammetry:- 0.4V terminates
Current potential:- 1.3V, maximum potential:- 0.4V, potential minimum:- 1.3V, sweep speed:0.03V/s, cycle-index:25 times, sampling
Interval:0.001V, sedimentation time:2s, sensitivity:10-4Under conditions of electropolymerization is carried out to the polymer fluid of above-mentioned acquisition, it is to be polymerized
After completing the conductive carmine molecular engram film of a layer polypropylene amide is formed in glass carbon working electrode surface;
Above-mentioned glass carbon working electrode is placed in deionized water and is rinsed 2-3 times, after by itself and saturation Ag/AgCl reference electrodes
Electrode is inserted into together with platinum filament in the sodium hydroxide solution that substance withdrawl syndrome is 0.1mol/L, three electrodes are by electrochemical operation
It stands connection;It is initial potential in each technical parameter with the function of differential pulse voltammetry:0.0V terminates current potential:1.0V, increment
Voltage:0.004V, amplitude:0.05V, pulse width:0.2V, sampling width:0.0167s, pulse period:0.5s, sedimentation time:
2s, sensitivity:10-6Under conditions of carry out electrochemistry and elute 5-10 time to get the conductive famille rose molecular engram film of polyacrylamide.
More than chemical reagent is that analysis is pure, Sinopharm Chemical Reagent Co., Ltd.'s production.
It is scanned through Electronic Speculum (S-3400 type SEM scanning electron microscope, Hitachi, Japan), cyclic voltammetry scan method (CHI600D
Electrochemical workstation, Shanghai Chen Hua electrochemical apparatus company) and electrochemical impedance spectroscopy (CHI600D electrochemical workstations, Shanghai occasion
Magnificent electrochemical apparatus company) analysis and characterization see Fig. 1-4, it is found that the polyacrylamide electroconductive molecule trace of above-mentioned synthesis
The following structural features of film:
By being compared with the blank polyacrylamide film without doping representated by Fig. 1, the polypropylene representated by Fig. 2
The apparent out-of-flatness of amide electroconductive molecule trace film surface, there is gully.
It can be observed compared with blank glass-carbon electrode and blank polyacrylamide membrane electrode by Fig. 3, hexacyanoferrate
Redox peak current on polyacrylamide electroconductive molecule blotting membrane is significantly greater than on blank polyacrylamide film, and
It is similar on blank glass-carbon electrode.This demonstrate that polyacrylamide electroconductive molecule blotting membrane is compared to blank polyacrylamide
Film, electric conductivity are improved significantly.
It can be observed compared with blank glass-carbon electrode and blank polyacrylamide membrane electrode by Fig. 4, polyacrylamide
The electrochemical impedance of electroconductive molecule blotting membrane is significantly less than blank polyacrylamide film, and is similar to blank glass-carbon electrode.And
It is about 50ohm that its impedance, which can be calculated,.Gather this further demonstrates polyacrylamide electroconductive molecule blotting membrane compared to blank
Acrylamide film, electric conductivity are improved significantly.
Fig. 5 describes the schematic diagram of polyacrylamide electroconductive molecule blotting membrane preparation process, by can be seen that template on figure
Molecule forms a co-polymer membrane with function monomer and the common electropolymerization of other components, then will by way of electrochemistry elution
Template molecule elution, so as to leave a cavity to the selective response of template molecule.Utilize this cavity, it is possible to realize
Quantitative detection is carried out to corresponding templates molecule.
Embodiment 2
The conductive carmine molecular engram film of polyacrylamide is for carmine quantitative detection
Specifically comprise the following steps:
(1), the preparation of the conductive carmine molecular engram film electrode of polyacrylamide:
With example 1.
(2), carmine red standard solution and the configuration to be measured containing carmine ingredient juice solution:
Compound concentration is respectively the carmine red standard solution of 2mg/L, 4mg/L, 6mg/L, 8mg/L, 10mg/L, 12mg/L,
It takes commercially available containing carmine ingredient juice solution 10mL, 50mL is settled to deionized water;
(3), using the conductive carmine molecular engram film electrode of polyacrylamide, electrochemical workstation (Shanghai Chen Hua is utilized
Instrument company's production CHI660D types electrochemical workstation) Electrochemical Detection is carried out, it is observed using differential pulse voltammetry (DPV)
The electrochemical response of carmine molecule:
The glass carbon working electrode point that seven are attached with step (1) and prepare the conductive carmine molecular engram film of polyacrylamide
It in each solution prepared by other inserting step (2), stirs half an hour, fully after absorption, is placed in deionized water and rinses 2-3 times,
Above-mentioned rinsing good seven glass carbon working electrodes, saturation Ag/AgCl reference electrodes and platinum filament are inserted into electrode together successively afterwards
Substance withdrawl syndrome is in the hac buffer (pH=4) of 0.1mol/L, and three electrodes are connected by electrochemical workstation;With difference
The function of pulse voltammetry is initial voltage in each technical parameter:0.0V, end voltage:1.0V, incremental voltage:0.004V,
Amplitude:0.05V, pulse width:0.2V, sampling width:0.0167s, pulse period:0.5s, sedimentation time:2s, sensitivity:
10-6Under conditions of be successively read peak current corresponding to above-mentioned seven glass carbon working electrode respectively;
(4), the drafting of standard curve
Using peak current as ordinate, carmine concentration corresponding to carmine red standard solution is abscissa, draw peak current with
The curve between carmine concentration corresponding to carmine red standard solution, fitting obtains carmine concentration and the standard of peak current is bent
Line equation;
(5), measure containing content carmine in carmine ingredient juice solution
The peak current containing carmine ingredient juice solution measured in step (3) is substituted into carmine obtained by step (4)
In concentration and the calibration curve equation of peak current, concentration carmine in solution to be measured is calculated, then according in step (2)
Extension rate, so as to which content carmine in solution to be measured be obtained.
On the above-mentioned polyacrylamide electroconductive molecule trace membrane electrode for being adsorbed with various various concentration carmine red standard solution
Differential pulse voltammetry respondent behavior and it is adsorbed with the polyacrylamide electroconductive molecule blotting membrane containing carmine ingredient juice solution
Differential pulse voltammetry respondent behavior on electrode, result is as shown in Figure 7 and Figure 8.
Differential pulse voltammetry is utilized as can be seen from Figure 7, is adsorbed with the polypropylene of various concentration carmine red standard solution
Carmine molecule on the amide electroconductive molecule trace membrane electrode equal appearance at 0.7V, and with carmine red standard solution concentration
Increase peak current be sequentially increased.As can be seen from Figure 8 using differential pulse voltammetry, it is adsorbed with containing carmine ingredient fruit juice
Carmine molecule on the polyacrylamide electroconductive molecule trace membrane electrode of the solution also appearance at 0.7V.It has been indicated above this hair
Bright polyacrylamide electroconductive molecule blotting membrane combination differential pulse voltammetry has preferable reappearance for carmine detection.
Shown in curve from Fig. 9 between carmine concentration corresponding to the peak current of drafting and 6 carmine red standard solution,
Middle ordinate is peak current, and carmine concentration of the abscissa corresponding to carmine red standard solution is fitted and obtains carmine concentration
It is as shown in Figure 10 with the curvilinear equation of peak current, Y=-0.52X-0.24 (coefficient Rs2=0.984), will be adsorbed with containing rouge
Peak current data on the polyacrylamide electroconductive molecule trace membrane electrode of the red ingredient juice solution of fat are substituted into obtained by above-mentioned fitting
Curvilinear equation in, the carmine concentration in carmine sample solution to be measured is calculated.Then according to step (2) kermes to be measured
The extension rate of proof sheet product is 4.33mg/L, result and production so as to which concentration carmine in carmine sample to be measured be obtained
The carmine content 4mg/L that business provides is consistent in standard relative deviation allowed band.Have so as to show therefore this method
Good accuracy.
Above said content is only the basic explanation under present inventive concept, and what technical solution according to the invention was done appoints
What equivalent transformation, is within the scope of protection of the invention.
Claims (10)
1. a kind of preparation method of polyacrylamide electroconductive molecule blotting membrane, which is characterized in that be as follows, specific steps
It is as follows:
(1) by acrylamide, N, N- methylene diacrylamides, potassium peroxydisulfate and potassium nitrate mix in proportion, after stirring evenly, obtain
To mixed liquor A;Template molecule, dopamine D A and phytic acid PA are mixed in proportion, after stirring evenly, obtain mixed liquid B;It will mixing
After liquid A and mixed liquid B mixing polymer fluid is obtained after ultrasound condition pre-reaction;
(2) by glass carbon working electrode, saturation Ag/AgCl reference electrodes and platinum filament, to electrode, inserting step (1) is gathered together
It closes in liquid, three electrodes are connected by electrochemical workstation, electroinitiated polymerization reaction are carried out using cyclic voltammetry, after polymerization is completed
A layer polypropylene amide electroconductive molecule blotting membrane is formed in glass carbon working electrode surface;
(3) the glass carbon working electrode of the polyacrylamide electroconductive molecule blotting membrane of preparation is placed in deionized water and rinsed 2-3 times,
To remove the unpolymerized component that may be adsorbed;Afterwards by the good glass carbon working electrode of above-mentioned rinsing, saturation Ag/AgCl reference electrodes
Electrode is inserted into the sodium hydroxide solution of 0.05~0.15mol/L together with platinum filament, three electrodes are connected by electrochemical workstation;
Differential pulse voltammetry carries out electrochemistry elution to template, obtains polyacrylamide electroconductive molecule blotting membrane.
2. preparation method according to claim 1, which is characterized in that in step (1), acrylamide AA, N, N- methenes pair
The mass ratio of acrylamide MBA, potassium peroxydisulfate and potassium nitrate are (7~8):(2~3):(2~3):(4:5).
3. preparation method according to claim 1, which is characterized in that in step (1), template molecule, dopamine D A and plant
The mass ratio of sour PA is (5~6):28:(1~2).
4. preparation method according to claim 1, which is characterized in that in step (1), template molecule is famille rose.
5. preparation method according to claim 1, which is characterized in that in step (1), the volume of mixed liquor A and mixed liquid B
Than for 1:1~1:1.5.
6. preparation method according to claim 1, which is characterized in that in step (2), during cyclic voltammetry, initial electricity
Position:- 0.4V terminates current potential:- 1.3V, maximum potential:- 0.4V, potential minimum:- 1.3V, sweep speed:0.03V/s, Xun Huan time
Number:25 times~35 times, the sampling interval:0.001V, sedimentation time:2s~3s, sensitivity:10-4。
7. preparation method according to claim 1, which is characterized in that in step (3), in differential pulse voltammetry, initially
Current potential:0.0V terminates current potential:1.0V, incremental voltage:0.004V, amplitude:0.05V, pulse width:0.2V, sampling width:
0.0167s, pulse period:0.5s, sedimentation time:2s, sensitivity:10-6。
8. a kind of polyacrylamide electroconductive molecule blotting membrane that preparation method according to one of claim 1~7 obtains.
9. polyacrylamide electroconductive molecule blotting membrane according to claim 8, which is characterized in that conductive point of polyacrylamide
The sub- a diameter of 1~3mm of blotting membrane;Polyacrylamide electroconductive molecule trace membrane impedance is 45~55ohm.
10. a kind of polyacrylamide electroconductive molecule blotting membrane according to claim 8 answering in carmine content detection
With, which is characterized in that application process is as follows:
(1), carmine red standard solution and the preparation to be measured containing carmine ingredient juice solution
Compound concentration is respectively that the carmine red standard solution of 2mg/L, 4mg/L, 6mg/L, 8mg/L, 10mg/L, 12mg/L take and contains
There is the solution 10mL to be measured of carmine ingredient, 50mL is settled to deionized water;
(2), the quantitative detection of carmine molecule
It it is prepared by the glass carbon working electrode difference inserting step (1) of the conductive carmine molecular engram film of seven attachment polyacrylamides
Each solution in, stir 20~40min, fully absorption after, be placed in deionized water and rinse 2-3 times, after successively by above-mentioned rinsing
Good seven glass carbon working electrodes, saturation Ag/AgCl reference electrodes and platinum filament is inserted into electrode substance withdrawl syndrome and is together
In the hac buffer of the pH=4 of 0.1mol/L, three electrodes are connected by electrochemical workstation;By differential pulse voltammetry into
Row measures, and is successively read the peak current corresponding to above-mentioned seven glass carbon working electrode respectively;The specific item of differential pulse voltammetry
Part is as follows:Initial voltage:0.0V, end voltage:1.0V, incremental voltage:0.004V, amplitude:0.05V, pulse width:0.2V,
Sampling width:0.0167s, pulse period:0.5s, sedimentation time:2s, sensitivity:10-6;
(3), the drafting of standard curve
Using peak current as ordinate, carmine concentration is abscissa corresponding to carmine red standard solution, draws peak current and kermes
The curve between carmine concentration corresponding to red standard solution, fitting obtain the standard curve side of carmine concentration and peak current
Journey;
(4), measure containing content carmine in carmine ingredient juice solution
The peak current of the solution to be measured containing carmine ingredient measured in step (2) is substituted into carmine dense obtained by step (3)
Degree is in the calibration curve equation of peak current, being calculated concentration carmine in solution to be measured, then according in step (1)
Extension rate, so as to which content carmine in solution to be measured be obtained.
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CN107174976A (en) * | 2017-05-18 | 2017-09-19 | 安徽众诚环境检测有限公司 | A kind of environment measuring modified polypropene hollow fiber filter membrane and preparation method thereof |
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