CN108103053A - The method that cationic montmorillonite is used for malignant cell DNA separating-purifyings - Google Patents
The method that cationic montmorillonite is used for malignant cell DNA separating-purifyings Download PDFInfo
- Publication number
- CN108103053A CN108103053A CN201710945146.7A CN201710945146A CN108103053A CN 108103053 A CN108103053 A CN 108103053A CN 201710945146 A CN201710945146 A CN 201710945146A CN 108103053 A CN108103053 A CN 108103053A
- Authority
- CN
- China
- Prior art keywords
- montmorillonite
- dna
- cationic
- malignant cell
- purifyings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Saccharide Compounds (AREA)
Abstract
The present invention relates to a kind of application of cationic montmorillonite in malignant cell DNA separating-purifyings, HeLa cell is cracked by using sodium dodecyl sulfate solution, cell pyrolysis liquid is obtained after heated, ultrasonic, then by cationic montmorillonite suspension, pH to 5.0~6.0 is adjusted with sodium acetate, progress DNA absorption in cell pyrolysis liquid is added to, is centrifuged after absorption, DNA desorptions are carried out with eluent washing lower sediment.The present invention is by being modified low-cost montmorillonite, modified obtained cationic montmorillonite can be rapidly and efficiently purification malignant cell DNA, malignant cell DNA extracted amounts can be regulated and controled on demand simultaneously, separating-purifying process will not damage the structure of malignant cell DNA.
Description
Technical field
The present invention relates to the separating-purifyings of malignant cell DNA, and in particular to a kind of cationic montmorillonite is pernicious
Application in DNA of tumor cell separating-purifying.
Background technology
Tumour is one of disease for seriously endangering human health.Tumour cell releases after necrosis, apoptosis can be in people
The Oncogenome dissociative DNA segment constantly cycled in body hematological system is known as ctDNA.CtDNA can non-invasively be used for tumour
Early diagnosis, orientation direction and Index for diagnosis etc., are a kind of extensive tumor markerses of application prospect.One 100g (3 ×
1010A cancer cell) tumour, daily the DNA of tumor cell there are about 3.3% enter blood circulation.Loads of the DNA as hereditary information
Body stores and the relevant important information of human body diseases.The detection for realizing malignant cell DNA can be that the disease of human body is walked
It is predicted to potential risk.
Tang Yini etc. (it is organic with inorganic modified montmorillonite to salmon sperm dna adsorpting characteristic research functional material 2012 years the
13 phases (43) roll up) pass through Fe3+And Al3+Poly- hydroxyl Fe/Al compounds are formed between montmorillonite layer and reach pillared effect, discussing should
Poly- hydroxyl Fe/Al montmorillonites to the adsorpting characteristic of salmon sperm dna, but not to the DNA after desorption can in cell successful expression
It discusses, while the Al-pillared montmorillonite pair prepared according to the sequence to DNA adsorbances in text, poly- hydroxyl Fe/Al
The adsorbance of DNA is less than raw material.The commercial kit for being currently able to successfully extract DNA and express includes centrifugal column kit
And magnetic bead kit, but presence is such as of high cost, is not easy to the defects of high-throughput operation for two kinds of kits.It is of the present invention
Montmorillonite there is material to be easy to get, is of low cost, without overt toxicity and to disliking as a kind of layer silicate mineral of natural output
The advantages of property DNA of tumor cell extracted amount can regulate and control on demand, modified cationic montmorillonite is to malignant cell DNA's
Adsorption efficiency reaches as high as 4.5 times of raw material or so, is expected to realize the early prediction of malignant tumour and reduces malignant tumour reagent
Box production cost.
The content of the invention
For being purified in existing diagnostic kit, model is single, is not easy to the sides such as high throughput operates and materials synthesis is of high cost
The problem of face, the present invention propose a kind of application of cationic montmorillonite in malignant cell DNA separating-purifyings, pass through
Low-cost montmorillonite is modified, the purification malignant tumour that modified obtained cationic montmorillonite can be rapidly and efficiently
Cell DNA, while malignant cell DNA extracted amounts can be regulated and controled on demand, separating-purifying process will not be thin to malignant tumour
The structure of born of the same parents DNA damages.
In order to achieve the above object, the present invention proposes a kind of cationic montmorillonite and is separated in malignant cell DNA
Application in purification, comprises the following steps：
(1) HeLa cell is cracked using sodium dodecyl sulfate solution, cell pyrolysis liquid is heated, it is ultrasonic after freeze
It deposits spare；
(2) it is suspension to match somebody with somebody cationic montmorillonite, adjusts pH to 5.0~6.0 with sodium acetate, adds in step (1) institute
It obtains cell pyrolysis liquid and carries out DNA absorption, centrifuge, take supernatant and measure DNA content therein, lower sediment is spare.
(3) with lower sediment obtained by eluent washing step (2), centrifuge, take supernatant and measure DNA therein
Content.
Heating temperature is 80~100 DEG C in the step (1), and the time is 3~10min, and ultrasonic amplitude 20%, power is
500W。
The mass fraction of sodium dodecyl sulfate solution is 1%~5% in the step (1).
The mass concentration of suspension is 20g/L~50g/L in the step (2).
Cationic montmorillonite is one kind in type lithium ion montmorillonite or aluminium ion type montmorillonite in the step (2).
The preparation method of the type lithium ion montmorillonite or aluminium ion type montmorillonite is：Ca-montmorillonite is added to carbonic acid
In lithium or aluminum nitrate solution, ca-montmorillonite is 1 with the mass ratio of lithium carbonate or aluminum nitrate：0.1~0.8, at 50~80 DEG C
Water bath with thermostatic control is stirred, and is centrifuged, is drying to obtain.
Eluent in the step (3) is one kind in deionized water, Tris-HCl solution or EDTA solution.
The advantages of the present invention are：
(1) the cationic bind-mechanism based on electronegative phosphate group in DNA helical structures Yu ca-montmorillonite surface,
A kind of cationic montmorillonite is prepared for, according to the double electrode layer theory of mineral in aqueous solution, calcium is regulated and controled by additional cation
The electric double layer of base montmorillonite promotes the dispersion effect of ca-montmorillonite, thin with malignant tumour so as to enhance cationic montmorillonite
The suction-operated of born of the same parents DNA rapidly and efficiently purifies malignant cell DNA.
(2) be modified with different valence state ion pair ca-montmorillonite, before modified after still exist in the form of an ion, for water
The adsorption/desorption of malignant cell DNA under solution system reduces malignant cell DNA separating-purifying material preparation costs,
The extracted amount of DNA according to actual conditions can on demand be regulated and controled simultaneously, can extract and high-throughput can also operate on a small quantity.
(3) the malignant cell DNA after absorption and desorption can transfect the successful expression into cell, separating-purifying mistake
Cheng Buhui damages the structure of malignant cell DNA.
Description of the drawings
Fig. 1 is the XRD diagram of subject cationic type montmorillonite.
During Fig. 2 is cation-modified, the corresponding cation-modified efficiency in ca-montmorillonite.
Fig. 3 is the adsorption efficiency of cationic montmorillonite
Fig. 4 is the malignant cell DNA transfection expression figures after desorption.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with the accompanying drawings and specific implementation
Example is described in further detail the present invention.It should be appreciated that specific embodiment described herein is only to explain the present invention, and
It is not used in the restriction present invention.
The chemical formula (Na, Ca) of term " ca-montmorillonite " in this specification0.33(Al,Mg)2[Si4O10](OH)2·
nH2O, in some forms, ca-montmorillonite is layer silicate mineral.
Montmorillonite in this specification embodiment is the standardized product of Sanding Sci. ＆ Tech. Co., Ltd., Zhejiang, and the place of production is Hubei Hubei Province
State.
Embodiment 1
Cell cracking：
HeLa cell is cultivated in the culture dish of a diameter of 6cm, the rear sodium dodecyl sulfate solution for adding in 500 μ L 2%
It is cracked, cell pyrolysis liquid moves into EP pipes and heats 5min at 80 DEG C, under the conditions of amplitude is 20%, power is 500W
Ultrasound freezes spare in -20 DEG C of refrigerator.
The preparation of type lithium ion montmorillonite：
Li is dissolved into 9mL deionized waters2CO30.23g adds in ca-montmorillonite 1.00g, at 60 DEG C water bath with thermostatic control react
24 h, centrifuge, 60 DEG C of dryings for 24 hours, it is closed be stored in it is spare in drying basin, labeled as 0.5Li-MMT.
DNA is adsorbed：
The 70 μ L of type lithium ion montmorillonite suspension of 30g/L are taken, add in 20 μ L cell pyrolysis liquids and 10 μ LpH as 5.0 vinegar
Acid sodium solution is centrifuged 2min under the centripetal acceleration of 5000g after reacting 2min, takes supernatant and surveyed with agargel electrophoresis
The content of fixed DNA therein.
DNA is desorbed：
The cationic montmorillonite of adsorption of DNA is centrifuged, gained sediment is scattered in 100 μ L deionized waters,
2min is centrifuged under the centripetal acceleration of 5000g after reaction 2min, take supernatant and is measured with agargel electrophoresis therein
The content of DNA.
Embodiment 2
Cell cracking：
HeLa cell is cultivated in the culture dish of a diameter of 6cm, the rear sodium dodecyl sulfate solution for adding in 500 μ L 2%
It is cracked, cell pyrolysis liquid moves into EP pipes and heats 5min at 90 DEG C, under the conditions of amplitude is 20%, power is 500W
Ultrasound freezes spare in -20 DEG C of refrigerator.
The preparation of type lithium ion montmorillonite：
Li is dissolved into 9mL deionized waters2CO30.47g adds in ca-montmorillonite 1.00g, at 60 DEG C water bath with thermostatic control react
24 h, centrifuge, 60 DEG C of dryings for 24 hours, it is closed be stored in it is spare in drying basin, labeled as Li-MMT.
DNA is adsorbed：
The 70 μ L of type lithium ion montmorillonite suspension of 30g/L are taken, add in 20 μ L cell pyrolysis liquids and 10 μ LpH as 5.0 vinegar
Acid sodium solution is centrifuged 2min under the centripetal acceleration of 5000g after reacting 2min, takes supernatant and surveyed with agargel electrophoresis
The content of fixed DNA therein.
DNA is desorbed：
The cationic montmorillonite of adsorption of DNA is centrifuged, it is 8.0 that gained sediment, which is scattered in 100 μ L pH,
In Tris-HCl solution, 2min is centrifuged under the centripetal acceleration of 5000g after reacting 2min, takes supernatant and with agar gel electricity
Swimming method measures the content of DNA therein.
Embodiment 3
Cell cracking：
HeLa cell is cultivated in the culture dish of a diameter of 6cm, the rear sodium dodecyl sulfate solution for adding in 500 μ L 2%
Cracked, cell pyrolysis liquid moves into EP pipes and at 100 DEG C heats 5min, amplitude be 20%, power is 500W conditions
Lower ultrasound freezes spare in -20 DEG C of refrigerator.
The preparation of type lithium ion montmorillonite：
Li is dissolved into 9mL deionized waters2CO30.71g adds in ca-montmorillonite 1.00g, at 60 DEG C water bath with thermostatic control react
24 h, centrifuge, 60 DEG C of dryings for 24 hours, it is closed be stored in it is spare in drying basin, labeled as 1.5Li-MMT.
DNA is adsorbed：
The 70 μ L of type lithium ion montmorillonite suspension of 30g/L are taken, add in 20 μ L cell pyrolysis liquids and 10 μ LpH as 5.0 vinegar
Acid sodium solution centrifuges 2min after reacting 2min under the centripetal acceleration of 5000g.It takes supernatant and is surveyed with agargel electrophoresis
The content of fixed DNA therein.
DNA is desorbed：
The cationic montmorillonite of adsorption of DNA is centrifuged, it is 8.0 that gained sediment, which is scattered in 100 μ LpH,
In EDTA solution, 2min is centrifuged under the centripetal acceleration of 5000g after reacting 2min, takes supernatant and with agargel electrophoresis
Method measures the content of DNA therein.
Embodiment 4
Cell cracking：
HeLa cell is cultivated in the culture dish of a diameter of 6cm, the rear sodium dodecyl sulfate solution for adding in 500 μ L 2%
It is cracked, cell pyrolysis liquid moves into EP pipes and heats 5min at 90 DEG C, under the conditions of amplitude is 20%, power is 500W
Ultrasound freezes spare in -20 DEG C of refrigerator.
The preparation of aluminium ion type montmorillonite：
It is dissolving Al (NO in 2 deionized waters to 9mLpH3)3·9H2O 0.47g, add in ca-montmorillonite 1.00g, 60 DEG C
Lower water bath with thermostatic control reaction for 24 hours, washing three times, separation, 60 DEG C of dryings for 24 hours, it is closed be stored in it is spare in drying basin, labeled as Al-
MMT。
DNA is adsorbed：
The 70 μ L of aluminium ion type montmorillonite suspension of 30g/L are taken, add in 20 μ L cell pyrolysis liquids and 10 μ LpH as 5.0 vinegar
Acid sodium solution is centrifuged 2min under the centripetal acceleration of 5000g after reacting 2min, takes supernatant and surveyed with agargel electrophoresis
The content of fixed DNA therein.
DNA is desorbed：
The cationic montmorillonite of adsorption of DNA is centrifuged, gained sediment is scattered in 100 μ L deionized waters,
2min is centrifuged under the centripetal acceleration of 5000g after reaction 2min, take supernatant and is measured with agargel electrophoresis therein
The content of DNA.
Comparative example 1
Cell cracking：
HeLa cell is cultivated in the culture dish of a diameter of 6cm, the rear sodium dodecyl sulfate solution for adding in 500 μ L 2%
Cracked, cell fragment and lysate move into EP pipes and heat 5min at 90 DEG C, amplitude be 20%, power 500W
Under the conditions of ultrasound, freeze spare in -20 DEG C of refrigerator.
Raw material ca-montmorillonite, labeled as Ca-MMT, adsorption of DNA：
The 70 μ L of ca-montmorillonite suspension of 30g/L are taken, add in 20 μ L cell pyrolysis liquids and 10 μ LpH as 5.0 sodium acetate
Solution centrifuges 2min under the centripetal acceleration of 5000g after reacting 2min, takes supernatant and measure it with agargel electrophoresis
In DNA content.
DNA is desorbed：
The cationic montmorillonite of adsorption of DNA is centrifuged, gained sediment is scattered in 100 μ L deionized waters,
2min is centrifuged under the centripetal acceleration of 5000g after reaction 2min, take supernatant and is measured with agargel electrophoresis therein
The content of DNA.
During Fig. 2 is cation-modified, the corresponding cation-modified efficiency in ca-montmorillonite.As shown in Figure 2, pass through
Atomic Emission Spectrometer AES measures the modified supernatant conversions of Li-MMT and Al-MMT and understands, lithium ion and aluminum ions modified effect
Rate is respectively 97% and 99%.
Fig. 3 is the adsorption efficiency of cationic montmorillonite.From the figure 3, it may be seen that the adsorption efficiency of Ca-MMT is about 4.42 μ g/
The adsorption efficiency of mg, 0.5Li-MMT are about 10.55 μ g/mg.The adsorption efficiency of Li-MMT is about 9.88 μ g/mg, 1.5Li-MMT
Adsorption efficiency be about 4.97 μ g/mg, Al-MMT 4.87 μ g/mg of adsorption efficiency.Cationic montmorillonite is to the adsorbance of DNA
Different degrees of promotion is shown, while each material shows certain DNA desorption effects.
Fig. 4 is the malignant cell DNA transfection expression figures after desorption.Mono- behavior control groups of Negative control,
Untransfected DNA.EGFP-c1plasmidpositive control be direct transfection Plasmid DNA positive control, plasmid expression
Cell fluoresced green afterwards.As shown in Figure 4, (eGFP-c1plasmid post are transfected to the DNA after desorption
Adsorption＆ desorption), there is green fluorescence, the DNA successful expressions of the desorption, entire extraction process will not be to disliking
The structure of property DNA of tumor cell damages.
Claims (7)
1. application of a kind of cationic montmorillonite in malignant cell DNA separating-purifyings, which is characterized in that including following
Step：
(1) add in sodium dodecyl sulfate solution in HeLa cell culture dish to be cracked, cell pyrolysis liquid is heated, ultrasonic
After freeze it is spare；
(2) it is suspension to match somebody with somebody cationic montmorillonite, and pH to 5.0~6.0 is adjusted with sodium acetate, is added in thin obtained by step (1)
Cellular lysate liquid carries out DNA absorption, centrifuges, takes supernatant and measure DNA content therein, lower sediment is spare；
(3) with lower sediment obtained by eluent washing step (2), centrifuge, take supernatant and measure DNA content therein.
2. application of the cationic montmorillonite according to claim 1 in malignant cell DNA separating-purifyings, special
Sign is, heating temperature is 80~100 DEG C in the step (1), and the time is 3~10min, ultrasonic amplitude 20%, and power is
500W。
3. application of the cationic montmorillonite according to claim 1 in malignant cell DNA separating-purifyings, special
Sign is that the mass fraction of sodium dodecyl sulfate solution is 1%~5% in the step (1).
4. application of the cationic montmorillonite according to claim 1 in malignant cell DNA separating-purifyings, special
Sign is that the mass concentration of suspension is 20g/L~50g/L in the step (2).
5. application of the cationic montmorillonite in malignant cell DNA separating-purifyings according to claim 1-2,
It is characterized in that, cationic montmorillonite is one kind in type lithium ion montmorillonite or aluminium ion type montmorillonite in the step (2).
6. application of the cationic montmorillonite according to claim 5 in malignant cell DNA separating-purifyings, special
Sign is that the preparation method of the type lithium ion montmorillonite or aluminium ion type montmorillonite is：Ca-montmorillonite is added in into lithium carbonate
Or in aluminum nitrate solution, ca-montmorillonite is 1 with the mass ratio of lithium carbonate or aluminum nitrate：0.1~0.8, it is permanent at 50~80 DEG C
Tepidarium stirring 0.5~for 24 hours, it centrifuges, is drying to obtain.
7. application of the cationic montmorillonite in malignant cell DNA separating-purifyings according to claim 1-2,
It is characterized in that, the eluent in the step (3) is one kind in deionized water, Tris-HCl solution or EDTA solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710945146.7A CN108103053B (en) | 2017-10-12 | 2017-10-12 | Method for separating and purifying DNA of malignant tumor cells by using cationic montmorillonite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710945146.7A CN108103053B (en) | 2017-10-12 | 2017-10-12 | Method for separating and purifying DNA of malignant tumor cells by using cationic montmorillonite |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108103053A true CN108103053A (en) | 2018-06-01 |
| CN108103053B CN108103053B (en) | 2021-12-24 |
Family
ID=62207077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710945146.7A Active CN108103053B (en) | 2017-10-12 | 2017-10-12 | Method for separating and purifying DNA of malignant tumor cells by using cationic montmorillonite |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108103053B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112708618A (en) * | 2020-12-30 | 2021-04-27 | 广州博江生物科技有限公司 | Lysate for quickly lysing cells, viruses and bacteria to extract nucleic acid |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE20207793U1 (en) * | 2002-05-17 | 2002-08-22 | Gl Biotech Gmbh | Kit for carrying out a method for nucleic acid extraction and nucleic acid purification |
| EP1527172A1 (en) * | 2002-05-17 | 2005-05-04 | GL Bio Tech GmbH | Method for nucleic acid extraction and nucleic acid purification |
| EP1960519A1 (en) * | 2005-12-09 | 2008-08-27 | Süd-Chemie Ag | Method for the sorption of at least one nucleic acid molecule using acid-activated phyllosilicates |
| CN105189750A (en) * | 2013-05-07 | 2015-12-23 | 精密公司 | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
-
2017
- 2017-10-12 CN CN201710945146.7A patent/CN108103053B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE20207793U1 (en) * | 2002-05-17 | 2002-08-22 | Gl Biotech Gmbh | Kit for carrying out a method for nucleic acid extraction and nucleic acid purification |
| EP1527172A1 (en) * | 2002-05-17 | 2005-05-04 | GL Bio Tech GmbH | Method for nucleic acid extraction and nucleic acid purification |
| EP1960519A1 (en) * | 2005-12-09 | 2008-08-27 | Süd-Chemie Ag | Method for the sorption of at least one nucleic acid molecule using acid-activated phyllosilicates |
| CN105189750A (en) * | 2013-05-07 | 2015-12-23 | 精密公司 | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
Non-Patent Citations (7)
| Title |
|---|
| FRANCK POLY等: "Differences between Linear Chromosomal and Supercoiled Plasmid DNA in Their Mechanisms and Extent of Adsorption on Clay Minerals", 《LANGMUIR》 * |
| GARY W. BEALL等: "Analysis of Oligonucleotide DNA Binding and Sedimentation Properties of Montmorillonite Clay Using Ultraviolet Light Spectroscopy", 《BIOMACROMOLECULES》 * |
| HONGYI XIE等: "Charge-Dependent Regulation in DNA Adsorption on 2D Clay Minerals", 《SCI REP.》 * |
| MARCO FRANCHI等: "Cations as mediators of the adsorption of nucleic acids on clay surfaces in prebiotic environments", 《ORIG LIFE EVOL BIOSPH》 * |
| PENG CAI等: "Binding and degradation of DNA on montmorillonite coated by hydroxyl aluminum species", 《COLLOIDS SURF B BIOINTERFACES》 * |
| 唐旖旎等: "有机与无机改性蒙脱石对鲑鱼精DNA吸附特征研究", 《功能材料》 * |
| 李香蓉等: "表面活性剂对蒙脱石吸附鲱鱼精DNA的影响", 《西南科技大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112708618A (en) * | 2020-12-30 | 2021-04-27 | 广州博江生物科技有限公司 | Lysate for quickly lysing cells, viruses and bacteria to extract nucleic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108103053B (en) | 2021-12-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vandeventer et al. | Multiphasic DNA adsorption to silica surfaces under varying buffer, pH, and ionic strength conditions | |
| CN1054354C (en) | Alumino-silicate derivatives | |
| CN106587005A (en) | Multi-step carbonized high quantum efficiency carbon quantum dot and preparation method thereof | |
| CN106904705B (en) | A kind of efficiently recyclable acidity contains As (V) wastewater treatment method | |
| CN103613169B (en) | The device of supergravity multistage sacrificial anode Electro-Fenton process process used water difficult to degradate and technique | |
| CN106621816B (en) | A kind of method that electric double layer carbon-based films continuously recycle lead ion in weak solution | |
| CN108103053A (en) | The method that cationic montmorillonite is used for malignant cell DNA separating-purifyings | |
| Wang et al. | Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing | |
| CN109569552A (en) | A kind of magnetic/non magnetic lanthanum carbonate sodium dephosphorization adsorbent and its synthetic method | |
| Chan et al. | Specific DNA extraction through fluid channels with immobilization of layered double hydroxides on polycarbonate surface | |
| CN203513300U (en) | Preparation system of high-purity lithium carbonate | |
| CN101519303B (en) | Water quality activation foil special for health preserving cup and preparation method thereof | |
| CN107243348A (en) | A kind of biological assisted synthesizing method of biocidal property photochemical catalyst | |
| CN106915860A (en) | A kind of method and device magnetized with electrolytic combination activated water | |
| CN103320627B (en) | Method for extracting potassium element from potassium-containing micaceous raw ore | |
| JP2014064566A (en) | Substance capable of charging/discharging as well as secondary battery and microbial secondary battery using the same | |
| CN104877679B (en) | The preparation method of tantalum disulfide quantum dot/nanometer sheet complex lead ion fluorescent probe | |
| CN205666968U (en) | Heating device for biochemistry | |
| Zhang et al. | Mesoporous carbon microparticles as a novel fluorescent sensing platform for thrombin detection | |
| CN206215257U (en) | A kind of thermostat water bath | |
| CN106323933B (en) | A kind of assay method of simple substance Se content | |
| CN104874355A (en) | Simple synthetic method for renewable magnetic carbon micro-sphere with effective adsorption property and application thereof | |
| CN104828829B (en) | A kind of thermal conductance closed cleans leaching equipment continuously | |
| Suresh et al. | Graphene quantum dots–hydrothermal green synthesis, material characterization and prospects for cervical cancer diagnosis applications: a review | |
| CN106753380B (en) | A kind of peach gum prepares the preparation method of rare earth up-conversion luminescence nano particle for template |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |