CN108096624A - Preparation method, material and the application of cell scaffold material - Google Patents
Preparation method, material and the application of cell scaffold material Download PDFInfo
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- CN108096624A CN108096624A CN201810057454.0A CN201810057454A CN108096624A CN 108096624 A CN108096624 A CN 108096624A CN 201810057454 A CN201810057454 A CN 201810057454A CN 108096624 A CN108096624 A CN 108096624A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/043—Proteins; Polypeptides; Degradation products thereof
- A61L31/046—Fibrin; Fibrinogen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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Abstract
Preparation method, material and the application of cell scaffold material belong to medical biomimetic material field.Preparation method includes：Fibrinogen is prepared with blood of human body.Fibrinogen is formed a film to obtain diaphragm, diaphragm is mixed with after the solution treatment containing Aprotinin with skin growth auxiliary agent.There is good biocompatibility by its cell scaffold material obtained, and be conducive to the growth of epidermal cell and stick.Application of the above-mentioned cell scaffold material in wound dressing, Antiadhesive film and hemostasis film is used to prepare prepares wound dressing, Antiadhesive film and hemostasis film as major ingredient or auxiliary material using the cell scaffold material, may advantageously facilitate skin healing.
Description
Technical field
The present invention relates to medical biomimetic material field, and more particularly to a kind of preparation method of cell scaffold material, material
And application.
Background technology
Biomimetic material refers to the various features of mimic biology or characteristic and the material developed, and it is right not only to simulate biology
The structure of elephant will more simulate its function.For tissue engineering bracket material as biomimetic material, referring to can be with tissue biopsy cell combination
And the different tissues of organism can be implanted into, and according to the specific material for substituting the function that tissue possesses.
Organizational project bioactivity tissue is, it is necessary to construct cytoskeleton compound, and timbering material can make seed cell
Effectively stick multiplication and growth.Tissue engineering bracket material includes：Bone, cartilage, blood vessel, nerve, skin and artificial organs, such as
The tissue stent material of liver,spleen,kidney, bladder etc..
But tissue engineering bracket material of the prior art is mainly propped up with the fibrinogen in the sources such as pig, ox
The preparation of frame material, there are allergy, heterogenous allosome immunological rejections.
The content of the invention
It is an object of the invention to provide a kind of preparation methods of cell scaffold material, pass through its cytoskeleton material obtained
Material has good biocompatibility, and is conducive to the growth of epidermal cell and sticks.
It another object of the present invention is to provide a kind of cell scaffold material, is made, had by above-mentioned preparation method
Standby biocompatibility is good and the characteristics of being conducive to the growth of epidermal cell and stick.
It is still another object of the present invention to provide a kind of above-mentioned cell scaffold materials to be used to prepare wound dressing, prevent adhesion
Application in film and hemostasis film, promotes skin healing.
The present invention is solved its technical problem and is realized using following technical scheme.
The present invention proposes a kind of preparation method of cell scaffold material, including：Fibrinogen is prepared with blood of human body；
Fibrinogen is formed a film to obtain diaphragm, diaphragm is mixed with after the solution treatment containing Aprotinin with skin growth auxiliary agent.
The present invention proposes a kind of cell scaffold material, is made according to the preparation method of above-mentioned cell scaffold material.
The present invention proposes a kind of above-mentioned cell scaffold material in wound dressing, Antiadhesive film and hemostasis film is used to prepare
Application.
The advantageous effect of the embodiment of the present invention is：
The preparation method of cell scaffold material provided by the invention is extracted fibrinogen as raw material using blood of human body and is used
In preparing timbering material.The non-heterologous source of fibrinogen, the good biocompatibility with human body.
Skin growth auxiliary agent can effectively facilitate the growth of epidermal cell and stick, and peomote skin healing.
Cell scaffold material provided by the invention is made by the preparation method of above-mentioned cell scaffold material, possesses biology
Compatibility is good and the characteristics of being conducive to the growth of epidermal cell and stick.
Above-mentioned cell scaffold material proposed by the present invention is in wound dressing, Antiadhesive film and hemostasis film is used to prepare
Using, using the cell scaffold material as major ingredient or auxiliary material prepare wound dressing, Antiadhesive film and hemostasis film, be conducive to promote
Into skin healing.
Description of the drawings
It in order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of scope, for those of ordinary skill in the art, without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the pictorial diagram of cell scaffold material provided in an embodiment of the present invention；
Fig. 2 is the electron microscope of cell scaffold material provided in an embodiment of the present invention；
Fig. 3 is live/dead of the human marrow mesenchymal stem cell in cell scaffold material provided in an embodiment of the present invention
Vital staining figure.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Preparation method, material and the application of the cell scaffold material of the embodiment of the present invention are specifically described below.
A kind of preparation method of cell scaffold material, including：
Fibrinogen is prepared with blood of human body；Fibrinogen is formed a film to obtain diaphragm, by diaphragm with containing the molten of Aprotinin
It is mixed after liquid processing with skin growth auxiliary agent.
The present invention uses blood of human body to extract fibrinogen for raw material and is used to prepare timbering material.This is extracted from human body blood
The non-heterologous source of fibrinogen of liquid makes the good biocompatibility of material and human body obtained.
Further, since timbering material would generally previously undergo all kinds of operations of row using patient, the blood of human body compared with
Goodly using discarded blood in the art in formerly being performed the operation using patient.The operation at least possesses advantages below：
Raw material is discarded object in operation, and discarded blood is turned waste into wealth, raw material is easy to get, advantageously reduce material preparation into
This, so as to reduce the medical expense of patient；Raw material sources are in using patient itself, and autologous is without immunological rejection, bio-compatible
Property is splendid, can promote sticking and being proliferated for cell；Simultaneously using self-blood without before use to that may be deposited in blood of human body
Virus spread etc. checked and screened out, security higher.
It operates and is made in preferred embodiments of the present invention, below fibrin reason：Blood of human body is centrifuged into obtain blood plasma,
Fibrinogen crude product is precipitated in the blood plasma precipitation method, is freeze-dried fibrinogen crude product.
By blood of human body centrifuge blood plasma operation, which eliminates the haemocyte contained in blood, convenient for blood plasma
Interior fibrinogen extracts, and is conducive to improve the purity of fibrinogen.
In preferred embodiments of the present invention, the rotating speed of centrifugally operated is 2800~3200r/min, the time for 14~
16min.Preferably, the rotating speed of centrifugally operated is 3000r/min, time 15min.The effect that it is centrifuged is good.
By the operation of blood plasma precipitation method precipitation fibrinogen crude product by fibrinogen from the blood plasma that centrifugation obtains
Separation, is convenient for the preparation of cell scaffold material.
In preferred embodiments of the present invention, the precipitation method be ethanol precipitation, make fibrinogen occur alcohol precipitation so as to
It is separated.
Above-mentioned ethanol precipitation is preferably comprised：By blood plasma and medical absolute ethyl alcohol with 9.5~10.5：1 volume ratio
Mixing is incubated 8~10mim under the conditions of -22~-20 DEG C.Preferably, the volume ratio of blood plasma and medical absolute ethyl alcohol is 10：1,
The temperature of incubation is -20 DEG C, can keep the bioactivity of fibrinogen, and alcohol precipitation is better.
It is after the completion of incubation it is preferred that thick to get fibrinogen with the rotating speed centrifugation substantially 10min of substantially 3000r/min
Product.
The diaphragm obtained after fibrinogen film forming can significantly decrease fiber egg through the solution treatment containing Aprotinin
White former degradation speed, while be conducive to growth and absorption of the epidermal cell on timbering material.
In preferred embodiments of the present invention, the concentration of Aprotinin is 4000~8000KU/ml in the solution containing Aprotinin,
Preferably 8000KU/ml, enzymatic treatment are better.
Since cell scaffold material is mainly used in dept. of dermatology made from this method, by after diaphragm enzymatic treatment with skin growth
Auxiliary agent mixes, and the dosage of skin growth auxiliary agent can suitably adjust selection according to the specific state of an illness of different patients.Can effectively it promote
Into epidermal cell growth and stick, peomote skin healing.
Further, in preferred embodiments of the present invention, skin growth auxiliary agent includes：Vitamin a race, vitamin b races,
Vitamin c race, gene peptide, growth factor, collagen and PRP.
Growth factor includes the combination of any one in bFGF or EGF or two kinds.BFGF refers to basic fibroblast growth
The factor, EGF refer to epithelical cell growth factor, and the two is used after facial injury, facial burn, facial attrition etc. and used,
It can shorten exudation reduction, healing time, pigmentation mitigation.
Vitamin c race can promote the synthesis of collagen.The generation of connective tissue is the premise of wound healing, vitamin C
Vulnerant wound can be led during shortage to be not easy to heal, so vitamin C is indispensable to the healing of wound.Meanwhile tie up life
The function well that plain C joints vitamin E confrontation cutis laxa recovers with color spot.
The addition of vitamin a race can reduce the generation of wrinkle.Vitamin A makes the wound of skin with collagen cooperation simultaneously
Ability enhancing is subjected to, while it additionally aids the healing of the symptoms such as ulcer.
Vitamin b races are conducive to alleviate the drying regime of skin, simultaneously for dry skin and infected with special effect
The anti-infection ability of skin can be improved.
PRP refers to high concentration thrombocyte plasma, plays an important role of hemostasis rapidly, analgesic, accelerating wound healing, can very big journey
Degree mitigates the formation of scar after the operation.It has auxiliary therapeutic action to skin diseases such as chronic ulcers simultaneously.
In preferred embodiments of the present invention, diaphragm operates film forming by electrostatic spinning by fibrinogen and is made.Using
Electrostatic spinning technique forms a film, and diaphragm obtained possesses high porosity, high-specific surface area, excellent material performance.
Further, electrostatic spinning operation includes：Fibrinogen is mixed into obtain electrostatic spinning solution with mixed solvent, it will
Electrostatic spinning solution is formed a film by electrostatic spinning apparatus.
Preferably, it is 8.5~9.5 that mixed solvent, which includes volume ratio,：1 HFP and MEM, fiber egg in electrostatic spinning solution
White former concentration is 90~120mg/ml.MEM refers to 10*MEM culture mediums.
It is highly preferred that it is 9 that mixed solvent, which includes volume ratio,：1 HFP and MEM, fibrinogen in electrostatic spinning solution
Concentration is 120mg/ml.Make the diaphragm uniform ground that film forming obtains.
Further, form a film after No. 22 passivity syringe needles that electrostatic spinning solution passes through electrostatic spinning apparatus, electrostatic spinning dress
The rotating speed for the collection circular shaft (diameter 6cm, length 20cm) put is 500~700r/min.Make to be collected in the Static Spinning for collecting circular shaft
Cortina piece is smooth consistent.
A kind of cell scaffold material is made by the preparation method of above-mentioned cell scaffold material, it is good to possess biocompatibility
The characteristics of well and being conducive to the growth of epidermal cell and stick.
A kind of application of above-mentioned cell scaffold material in wound dressing, Antiadhesive film and hemostasis film is used to prepare, is adopted
Wound dressing, Antiadhesive film and hemostasis film are prepared by the use of the cell scaffold material as major ingredient or auxiliary material, may advantageously facilitate skin
Healing.
Embodiment 1
A kind of preparation method of cell scaffold material, including：
S1, blood of human body is centrifuged into 15min with the rotating speed of 3000r/min, obtains blood plasma.
S2, by blood plasma and medical absolute ethyl alcohol with 10：1 volume ratio mixing, is incubated 9min under the conditions of -20 DEG C, with
The rotating speed centrifugation 10min of 3000r/min, obtains fibrinogen crude product.
S3, fibrinogen crude product is freeze-dried, obtains fibrinogen.
S4, by HFP and MEM culture mediums according to 9：1 volume ratio mixing, obtains mixed solvent.By fibrinogenolysis in
Mixed solvent, the concentration for obtaining fibrinogen is the electrostatic spinning solution of 120mg/ml.
S5, by electrostatic spinning solution by No. 22 passivity syringe needles of electrostatic spinning apparatus, in rotating speed be 600r/min, diameter
It forms a film on the collection circular shaft for being 20cm for 6cm, length, obtains diaphragm.
S6, diaphragm is handled with the solution containing Aprotinin that the concentration of Aprotinin is 8000KU/ml, and containing vitamin
A races, the skin growth auxiliary agent mixing of vitamin b races, vitamin c race, gene peptide, bFGF, collagen and PRP, obtain cell
Timbering material.
Embodiment 2
A kind of preparation method of cell scaffold material, including：
S1, blood of human body is centrifuged into 15min with the rotating speed of 3000r/min, obtains blood plasma.
S2, by blood plasma and medical absolute ethyl alcohol with 10：1 volume ratio mixing, is incubated 9min under the conditions of -20 DEG C, with
The rotating speed centrifugation 10min of 3000r/min, obtains fibrinogen crude product.
S3, fibrinogen crude product is freeze-dried, obtains fibrinogen.
S4, by HFP and MEM culture mediums according to 9：1 volume ratio mixing, obtains mixed solvent.By fibrinogenolysis in
Mixed solvent, the concentration for obtaining fibrinogen is the electrostatic spinning solution of 120mg/ml.
S5, by electrostatic spinning solution by No. 22 passivity syringe needles of electrostatic spinning apparatus, in rotating speed be 600r/min, diameter
It forms a film on the collection circular shaft for being 20cm for 6cm, length, obtains diaphragm.
S6, diaphragm is handled with the solution containing Aprotinin that the concentration of Aprotinin is 8000KU/ml, and containing vitamin
A races, the skin growth auxiliary agent mixing of vitamin b races, vitamin c race, gene peptide, EGF, collagen and PRP, obtain cell branch
Frame material.
Embodiment 3
A kind of preparation method of cell scaffold material, including：
S1, blood of human body is centrifuged into 16min with the rotating speed of 2800r/min, obtains blood plasma.
S2, by blood plasma and medical absolute ethyl alcohol with 9.5：1 volume ratio mixing, is incubated 8min under the conditions of -22 DEG C, with
The rotating speed centrifugation 10min of 3000r/min, obtains fibrinogen crude product.
S3, fibrinogen crude product is freeze-dried, obtains fibrinogen.
S4, by HFP and MEM culture mediums according to 8.5：1 volume ratio mixing, obtains mixed solvent.By fibrinogenolysis
In mixed solvent, the concentration for obtaining fibrinogen is the electrostatic spinning solution of 90mg/ml.
S5, by electrostatic spinning solution by No. 22 passivity syringe needles of electrostatic spinning apparatus, in rotating speed be 500r/min, diameter
It forms a film on the collection circular shaft for being 20cm for 6cm, length, obtains diaphragm.
S6, diaphragm is handled with the solution containing Aprotinin that the concentration of Aprotinin is 8000KU/ml, and containing vitamin
A races, the skin growth auxiliary agent mixing of vitamin b races, vitamin c race, gene peptide, bFGF, collagen and PRP, obtain cell
Timbering material.
Embodiment 4
A kind of preparation method of cell scaffold material, including：
S1, blood of human body is centrifuged into 16min with the rotating speed of 2800r/min, obtains blood plasma.
S2, by blood plasma and medical absolute ethyl alcohol with 9.5：1 volume ratio mixing, is incubated 8min under the conditions of -22 DEG C, with
The rotating speed centrifugation 10min of 3000r/min, obtains fibrinogen crude product.
S3, fibrinogen crude product is freeze-dried, obtains fibrinogen.
S4, by HFP and MEM culture mediums according to 8.5：1 volume ratio mixing, obtains mixed solvent.By fibrinogenolysis
In mixed solvent, the concentration for obtaining fibrinogen is the electrostatic spinning solution of 90mg/ml.
S5, by electrostatic spinning solution by No. 22 passivity syringe needles of electrostatic spinning apparatus, in rotating speed be 500r/min, diameter
It forms a film on the collection circular shaft for being 20cm for 6cm, length, obtains diaphragm.
S6, diaphragm is handled with the solution containing Aprotinin that the concentration of Aprotinin is 8000KU/ml, and containing vitamin
A races, the skin growth auxiliary agent mixing of vitamin b races, vitamin c race, gene peptide, EGF, collagen and PRP, obtain cell branch
Frame material.
Embodiment 5
A kind of preparation method of cell scaffold material, including：
S1, blood of human body is centrifuged into 14min with the rotating speed of 3200r/min, obtains blood plasma.
S2, by blood plasma and medical absolute ethyl alcohol with 10.5：1 volume ratio mixing, is incubated 10min under the conditions of -20 DEG C, with
The rotating speed centrifugation 10min of 3000r/min, obtains fibrinogen crude product.
S3, fibrinogen crude product is freeze-dried, obtains fibrinogen.
S4, by HFP and MEM culture mediums according to 9.5：1 volume ratio mixing, obtains mixed solvent.By fibrinogenolysis
In mixed solvent, the concentration for obtaining fibrinogen is the electrostatic spinning solution of 120mg/ml.
S5, by electrostatic spinning solution by No. 22 passivity syringe needles of electrostatic spinning apparatus, in rotating speed be 700r/min, diameter
It forms a film on the collection circular shaft for being 20cm for 6cm, length, obtains diaphragm.
S6, diaphragm is handled with the solution containing Aprotinin that the concentration of Aprotinin is 8000KU/ml, and containing vitamin
A races, the skin growth auxiliary agent mixing of vitamin b races, vitamin c race, gene peptide, bFGF, collagen and PRP, obtain cell
Timbering material.
Embodiment 6
A kind of preparation method of cell scaffold material, including：
S1, blood of human body is centrifuged into 14min with the rotating speed of 3200r/min, obtains blood plasma.
S2, by blood plasma and medical absolute ethyl alcohol with 10.5：1 volume ratio mixing, is incubated 10min under the conditions of -20 DEG C, with
The rotating speed centrifugation 10min of 3000r/min, obtains fibrinogen crude product.
S3, fibrinogen crude product is freeze-dried, obtains fibrinogen.
S4, by HFP and MEM culture mediums according to 9.5：1 volume ratio mixing, obtains mixed solvent.By fibrinogenolysis
In mixed solvent, the concentration for obtaining fibrinogen is the electrostatic spinning solution of 120mg/ml.
S5, by electrostatic spinning solution by No. 22 passivity syringe needles of electrostatic spinning apparatus, in rotating speed be 700r/min, diameter
It forms a film on the collection circular shaft for being 20cm for 6cm, length, obtains diaphragm.
S6, diaphragm is handled with the solution containing Aprotinin that the concentration of Aprotinin is 8000KU/ml, and containing vitamin
A races, the skin growth auxiliary agent mixing of vitamin b races, vitamin c race, gene peptide, EGF, collagen and PRP, obtain cell branch
Frame material.
Cell scaffold material made from Examples 1 to 6 is tested, the good biocompatibility with human skin has
Growth beneficial to epidermal cell and stick, skin healing speed is accelerated.
Test example
Cell scaffold material is made in the preparation method of the cytoskeleton provided according to embodiment, as shown in Figure 1, its electron microscope
As shown in Figure 2.Figure it is seen that cell scaffold material made from the embodiment of the present invention has good aperture and porosity,
Its large specific surface area is conducive to sticking and growing for cell.
Fig. 3 is live/dead vital staining figure of the human marrow mesenchymal stem cell in cell scaffold material obtained,
Middle RED sector represents dead cell, and green portion represents living cells, it can be seen from the figure that cell is made in the embodiment of the present invention
Cell scaffold material on compatibility it is good, good can grow.
In conclusion the preparation method of the cell scaffold material of the present invention, fibrin is extracted by raw material of blood of human body
It is originally intended to prepare timbering material.The non-heterologous source of fibrinogen, the good biocompatibility with human body.Skin growth auxiliary agent can
It effectively facilitates the growth of epidermal cell and sticks, peomote skin healing.
The cell scaffold material of the present invention, is made by the preparation method of above-mentioned cell scaffold material, possesses bio-compatible
Property good, the characteristics of being conducive to the growth of epidermal cell and sticking and the advantages that accelerate skin healing speed.
The above-mentioned cell scaffold material of the present invention answering in wound dressing, Antiadhesive film and hemostasis film is used to prepare
With, using the cell scaffold material as major ingredient or auxiliary material prepare wound dressing, Antiadhesive film and hemostasis film, may advantageously facilitate
Skin healing.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of preparation method of cell scaffold material, which is characterized in that it includes：Fibrinogen is prepared with blood of human body；
The fibrinogen is formed a film to obtain diaphragm, the diaphragm is mixed with after the solution treatment containing Aprotinin with skin growth auxiliary agent
It closes.
2. the preparation method of cell scaffold material according to claim 1, which is characterized in that the skin growth auxiliary agent bag
It includes：Vitamin a race, vitamin b races, vitamin c race, gene peptide, growth factor, collagen and PRP, the growth factor
Combination including any one in bFGF or EGF or two kinds.
3. the preparation method of cell scaffold material according to claim 1, which is characterized in that the diaphragm is by the fiber
Proteinogen is made by electrostatic spinning operation film forming, and electrostatic spinning operation includes：The fibrinogen is mixed with mixed solvent
Electrostatic spinning solution is closed to obtain, the electrostatic spinning solution is formed a film by electrostatic spinning apparatus；The mixed solvent includes volume
Than for 8.5~9.5：1 HFP and MEM, the concentration of fibrinogen described in the electrostatic spinning solution are 90~120mg/
ml；Preferably, it is 9 that the mixed solvent, which includes volume ratio,：1 HFP and MEM, fiber egg described in the electrostatic spinning solution
White former concentration is 120mg/ml.
4. the preparation method of cell scaffold material according to claim 3, which is characterized in that the electrostatic spinning solution leads to
It forms a film after crossing No. 22 passivity syringe needles of the electrostatic spinning apparatus, the rotating speed of the collection circular shaft of the electrostatic spinning apparatus is 500
~700r/min.
5. the preparation method of cell scaffold material according to claim 1, which is characterized in that the solution containing Aprotinin
The concentration of middle Aprotinin is 4000~8000KU/ml.
6. the preparation method of cell scaffold material according to claim 1, which is characterized in that the fibrin reason with
Lower operation is made：The blood of human body is centrifuged into obtain blood plasma, fibrinogen crude product, freezing is precipitated in the blood plasma precipitation method
The dry fibrinogen crude product.
7. the preparation method of cell scaffold material according to claim 6, which is characterized in that the rotating speed of centrifugation for 2800~
3200r/min, time are 14~16min；Preferably, the rotating speed of centrifugation be 3000r/min, time 15min.
8. the preparation method of cell scaffold material according to claim 6, which is characterized in that the precipitation method include：By described in
Blood plasma is with medical absolute ethyl alcohol with 9.5~10.5：1 volume ratio mixing, is incubated 8~10mim under the conditions of -22~-20 DEG C.
9. a kind of cytoskeleton material according to made from the preparation method of claim 1~8 any one of them cell scaffold material
Material.
10. cell scaffold material the answering in wound dressing, Antiadhesive film and hemostasis film is used to prepare described in claim 9
With.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810057454.0A CN108096624A (en) | 2018-01-22 | 2018-01-22 | Preparation method, material and the application of cell scaffold material |
Applications Claiming Priority (1)
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