CN108079030A - Application of the seedling medicine Radix Chimonanthi praecocis in anti-AD products are prepared - Google Patents
Application of the seedling medicine Radix Chimonanthi praecocis in anti-AD products are prepared Download PDFInfo
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- CN108079030A CN108079030A CN201810079646.1A CN201810079646A CN108079030A CN 108079030 A CN108079030 A CN 108079030A CN 201810079646 A CN201810079646 A CN 201810079646A CN 108079030 A CN108079030 A CN 108079030A
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- acetic acid
- iron chopsticks
- seedling medicine
- acid ethyl
- ethyl ester
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Animal Behavior & Ethology (AREA)
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- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention belongs to medicine and pharmacology technical fields, disclose application of the seedling medicine Radix Chimonanthi praecocis in anti-AD products are prepared, and utilize the drug for the treatment of AD prepared by seedling medicine iron chopsticks acetic acid ethyl ester extract；The preparation method of seedling medicine iron chopsticks acetic acid ethyl ester extract includes：Iron chopsticks are washed after reject sundries and dries, crush, after powder is taken to impregnate 16h with 70% ethyl alcohol, 70% alcohol heat reflux extracts 5h, extracting solution is concentrated after filtering, it is extracted with ethyl acetate, extract liquor is distinguished into rotary evaporation, is concentrated under reduced pressure, it is less than in temperature on 65 DEG C of water-baths and volatilizes solvent, obtains acetic acid ethyl ester extract.The seedling medicine iron chopsticks Ethyl acetate fraction of the present invention has anti-AD activity.
Description
Technical field
The invention belongs to medicine and pharmacology technical field more particularly to a seedling medicine Radix Chimonanthi praecocis in anti-AD products are prepared
Application.
Background technology
Seedling medicine iron chopsticks are Calycanthaceae wax-cakes bait vegetable wax plum Chimonanthus praecox (Linn.) Link. and mountain
The drying radicula of wax plum Chimonan nitens Oliv..Different name is also known as iron steel fork, watt bird bavin, is 2003 editions《Guizhou Province's Chinese medicine
Material, Ethnic crude drugs quality standard》Record kind.Senile dementia main clinical manifestation is the progressive based on recent memory obstacle
Hypophrenia and Psychomotor disorders, this sick pathogenic factor is intricate, pathogenesis still end is fully apparent from, modern medicine pair
The effective treatment means of shortage.The drug of the listing treatment of U.S. FDA approval at present AD is almost anticholinesterase, far
Phase curative effect is unsatisfactory, and is chemicals, has certain liver and gastrointestinal side effect.At present clinical treatment mainly for
Disease symptoms are treated, but cannot change the occurrence and development process of disease, therefore, in the case where disease mechanisms are unknown,
Brand-new medicine is found as current hot spot.China's traditional medicine focuses on organic conception and diagnosis and treatment, and traditional medicine
Object clinical practice is of long duration, have the characteristics that efficiently, low toxicity, the clinical effect of Mutiple Targets, therefore, based on it is now recognized that morbidity machine
Perhaps, system can excavate new medicine from the ethnic drug treasure-house of China.
In conclusion problem existing in the prior art is：Up to the present, it is clinical also to lack since AD pathogenesis is unclear
The specific medicament of weary treatment DA, drug therapy is mostly anticholinesterase, but mainly for clinical symptoms, to the hair of disease
Raw, evolution lacks effective prevention；Other are directed to the research of seedling medicine iron chopsticks, also without finding seedling medicine iron chopsticks ethyl acetate
Position has a correlative study of anti-AD activity, and without utilizing the preparation of this characteristic for treating the drug of AD.
The content of the invention
In view of the problems of the existing technology, the present invention provides a seedling medicine Radix Chimonanthi praecocis to prepare anti-AD products
In application.Present invention discover that seedling medicine iron chopsticks ethyl acetate extract has anti-AD activity.
The present invention be achieved in that it is based on traditional conventional medicament clinical practice of long duration, efficiently, low toxicity, Mutiple Targets
Clinical effect feature presses down the anti-oxidant of iron chopsticks acetic acid ethyl ester extract, cholinesterase with reference to early period and improves animal learning
Memory capability related activity research, a kind of drug of the treatment AD prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract of invention.
Another object of the present invention is to provide a kind for the treatment of AD prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract
Drug preparation method, the preparation method includes：
Iron chopsticks are washed after reject sundries and dries, crush, after powder is taken to impregnate 16h with 70% ethyl alcohol, 70% ethyl alcohol heat
Refluxing extraction 5h, concentrates extracting solution after filtering, is extracted with ethyl acetate, and recycling design obtains acetic acid ethyl ester extract to doing.
Advantages of the present invention and good effect are：1. though current drug has certain effect, can improve AD pathology late periods suffers from
The cognition of person and memory disorders, but the pathology and disease of AD can not be inhibited.Therefore it is new, have be different from existing drug master
The medicament research and development for wanting mechanism of action is still emphasis.Mainly act on that cholinesterase is different from existing drug, the seedling medicine in the present invention
Iron chopsticks ethyl acetate extract still has apparent anti-oxidant reduction oxidative injury in addition to inhibiting with cholinesterase, into
And play the role of improving AD animal pattern ability of learning and memory.2. oxidative stress is another theory of the main mechanism of AD morbidities,
Seedling medicine iron chopsticks ethyl acetate extract has antioxidation in the present invention, plays an important role of anti-oxidation stress, with inhibiting choline
Esterase effect collaboration, having enhances anti-AD effects.3. the present invention comes from conventional medicament, there is the novelty found, at present not yet
It was found that correlative study according to the present invention.
Description of the drawings
Fig. 1 is the preparation method flow chart of seedling medicine iron chopsticks acetic acid ethyl ester extract provided in an embodiment of the present invention.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
Present invention discover that seedling medicine iron chopsticks ethyl acetate extract has anti-AD activity.
The embodiment of the present invention provides a kind of drug of the treatment AD prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract.
The embodiment of the present invention provides the preparation of the drug of the treatment AD prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract
Method, the preparation method include：
The present invention is further described with reference to concrete analysis.
The preparation of test sample provided in an embodiment of the present invention：
Iron chopsticks 2kg is washed after reject sundries and dries, crush, 70% ethyl alcohol of its powder 250.0g is taken, in 78~80
When DEG C refluxing extraction 2~3 is small, extracting solution is filtered, is concentrated under reduced pressure, the medicinal extract being evaporated in evaporating dish about 20.0g, i.e. alcohol extract are obtained
Cream rate is 8%.
As shown in Figure 1, the preparation method of seedling medicine iron chopsticks acetic acid ethyl ester extract provided in an embodiment of the present invention, including：
S101：Iron chopsticks are washed after reject sundries and dries, crush, after its powder is taken to impregnate 16h with 70% ethyl alcohol, 70%
Alcohol heat reflux extracts 5h, concentrates extracting solution after filtering；
S102：It is extracted with ethyl acetate, by extract liquor recycling design to doing, obtains acetic acid ethyl ester extract.
With reference to concrete analysis, the invention will be further described.
Activity rating：
Ability of learning and memory (water maze laboratory)：
D- galactolipins 60mg/ (kgd) (normal saline) and gavage AlCl are subcutaneously injected using mouse neck35mg/
(kgd) (distilled water preparation) 1 time a day, replicates Model of Dementia in continuous 60 days.Continuously give iron chopsticks acetic acid ethyl ester extract
30 days, detect ability of learning and memory in mice.
Water maze incubation period after 1 mouse modeling of table, after administration compares
From table 1 it follows that Normal group mouse has just learned searching platform in 2 days, incubation period tends to be steady；
After modeling 60 days, compared with Normal group each group Mice water maze incubation period be obviously prolonged, illustrate ability of learning and memory in mice
There is obstacle, modeling success；After administration, compared with Normal group, from the 1st day, model group mouse found platform incubation period
It is obviously prolonged (P<0.01) it is unstable, though there is shortening trend in incubation period；Compared with model group, from first day, iron chopsticks second
Acetoacetic ester position high and low dose group mouse searching platform incubation period is obviously shortened (P<0.01).Show iron chopsticks ethyl acetate
Extraction position plays an important role of to improve model mice ability of learning and memory.
Anti-oxidant, inhibition cholinesterase activity experiment：
Iron chopsticks Ethyl acetate fraction is to AD model mice brain tissues SOD activity, MDA contents, TCHE activity influences.
The results show that SOD activity declines in model group Mice brain tissues, MDA rises show that free radical increases, and antioxidation weakens.
In ethyl acetate extract group Mice brain tissues:SOD activity raises (P compared with model group<0.05), MDA contents and model group are small
Mouse relatively has reduction trend (P<0.05), show that iron chopsticks Ethyl acetate fraction has the anti-oxidant energy of rise mouse dementia
Power reduces damage of the oxygen radical to body.Acetylcholine esterase active drops compared with model group in administration group Mice brain tissues
Low (P<0.05), show that iron chopsticks ethyl acetate extract can protect cerebral anoxia to damage, improve memory capability (table 2).
2 iron chopsticks Ethyl acetate fraction of table influences dementia mice brain tissue
Compared with model group：*P<0.05, compared with model group：#P<0.05, compared with model group：**P<0.05。
Conclusion：
Seedling medicine iron chopsticks Ethyl acetate fraction has anti-AD activity.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (3)
1. a kind of drug of the treatment AD prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract.
2. a kind of preparation side of the drug as described in claim 1 that control AD prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract
Method, which is characterized in that the preparation method includes：
Iron chopsticks 2.0kg is washed with water after reject sundries and dries in the shade, crush, after its powder is taken to impregnate 16h with 70% ethyl alcohol, 70% second
Alcohol heating and refluxing extraction 3 times is recovered under reduced pressure ethyl alcohol to no alcohol taste, is extracted with ethyl acetate, by extract liquor recycling design to doing, obtains
To acetic acid ethyl ester extract 44g, yield 2.2%.
3. a kind of seedling medicine of the drug of the treatment AD as described in claim 1 prepared using seedling medicine iron chopsticks acetic acid ethyl ester extract
The preparation method of iron chopsticks acetic acid ethyl ester extract, is characterized in that, the preparation side of the seedling medicine iron chopsticks acetic acid ethyl ester extract
Method includes：
Iron chopsticks are washed after reject sundries and dries, crush, after powder is taken to impregnate 16h with 70% ethyl alcohol, 70% alcohol heat reflux
Extraction 3 times, common 5h is recovered under reduced pressure ethyl alcohol to no alcohol taste, is extracted with ethyl acetate, by extract liquor recycling design to doing, obtains second
Acetoacetic ester extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810079646.1A CN108079030B (en) | 2018-01-26 | 2018-01-26 | Application of Miao medicine radix Chimonanthi Praecocis extract in preparation of anti-AD product |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810079646.1A CN108079030B (en) | 2018-01-26 | 2018-01-26 | Application of Miao medicine radix Chimonanthi Praecocis extract in preparation of anti-AD product |
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| Publication Number | Publication Date |
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| CN108079030A true CN108079030A (en) | 2018-05-29 |
| CN108079030B CN108079030B (en) | 2021-02-26 |
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| CN201810079646.1A Expired - Fee Related CN108079030B (en) | 2018-01-26 | 2018-01-26 | Application of Miao medicine radix Chimonanthi Praecocis extract in preparation of anti-AD product |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112656826A (en) * | 2021-01-29 | 2021-04-16 | 上海和黄药业有限公司 | Myocardial cell hypoxia injury medicine and preparation method and application thereof |
| CN112675207A (en) * | 2021-01-13 | 2021-04-20 | 上海和黄药业有限公司 | Medicine suitable for protecting nerve cells and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090238902A1 (en) * | 2006-04-19 | 2009-09-24 | Jianxun Liu | Chinese medicine composition and preparation method and use thereof |
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2018
- 2018-01-26 CN CN201810079646.1A patent/CN108079030B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090238902A1 (en) * | 2006-04-19 | 2009-09-24 | Jianxun Liu | Chinese medicine composition and preparation method and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| 杨健: ""铁筷子根茎化学成分的研究"", 《中国优秀硕士学位论文全文数据库》 * |
| 段亚平等: ""铁筷子总黄酮对D-半乳糖致衰小鼠的抗衰老作用研究"", 《 华西药学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112675207A (en) * | 2021-01-13 | 2021-04-20 | 上海和黄药业有限公司 | Medicine suitable for protecting nerve cells and preparation and application thereof |
| CN112656826A (en) * | 2021-01-29 | 2021-04-16 | 上海和黄药业有限公司 | Myocardial cell hypoxia injury medicine and preparation method and application thereof |
| CN112656826B (en) * | 2021-01-29 | 2022-03-11 | 上海和黄药业有限公司 | Myocardial cell hypoxia injury medicine and preparation method and application thereof |
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Granted publication date: 20210226 Termination date: 20220126 |