CN108077585A - A kind of feed addictive for alleviating jewfish er stress and preparation method and application - Google Patents
A kind of feed addictive for alleviating jewfish er stress and preparation method and application Download PDFInfo
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- CN108077585A CN108077585A CN201810147688.4A CN201810147688A CN108077585A CN 108077585 A CN108077585 A CN 108077585A CN 201810147688 A CN201810147688 A CN 201810147688A CN 108077585 A CN108077585 A CN 108077585A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to a kind of feed addictives for alleviating jewfish er stress and preparation method and application, belong to field of fodder.The feed addictive of every 100 parts by weight includes the alfalfa saponin of 20 30 parts by weight, the Methionine Chelated Zinc of 10 20 parts by weight, the taurine of 5 15 parts by weight, the kudzu-vine root powder of 20 30 parts by weight and the grape pip procyanidin of 5 10 parts by weight, and surplus is carrier.The raw material of the feed addictive is safe, without hazard residue, and condition is created for production green feed and aquatic products.Its preparation method is：Above-mentioned raw materials are mixed by proportioning.This preparation method is simple, and condition controllability is good, and stability is good, has a good application prospect.Above-mentioned feed addictive is applied to prepare jewfish feed, jewfish liver oxidation resistance can be improved, reduce endoplasmic reticulum oxidative damage, binding protein expression is reduced, alleviates er stress, maintain the stable state of endoplasmic reticulum.
Description
Technical field
The present invention relates to field of fodder, and more particularly to a kind of feed addictive and its system for alleviating jewfish er stress
Preparation Method and application.
Background technology
In recent years, cultivate jewfish fatty liver is frequently Universal Problems that aquaculture faces, the especially high warm season
Section jewfish is susceptible to fatty liver.Fatty liver not only results in the jewfish speed of growth and declines with feed conversion rate, can also reduce sea
The anti-stress ability of perch, once jewfish by high temperature and high ammonia nitrogen etc. stress, easy mortality, to jewfish cultivation bring it is huge
Economic loss.
It is very important to show that er stress plays the role of in the generation and harm of fatty liver for numerous researchs at present,
Er stress with fatty liver generation and development.Er stress can cause liver fat that cannot effectively be transported to liver
Outer tissue, so as to cause the excess accumulation of liver fat.In addition, er stress also results in cell oxidative damage and apoptosis,
It is reduced so as to cause the anti-stress ability of jewfish.
Therefore, the feed addictive that exploitation can alleviate jewfish er stress has very important meaning.
The content of the invention
It is an object of the invention to provide it is a kind of alleviate jewfish er stress feed addictive, the feed addictive
Raw material is safe, without hazard residue, and condition is created for production green feed and aquatic products.
The second object of the present invention is to provide a kind of preparation method of above-mentioned feed addictive, and the preparation method is simple,
Condition controllability is good, and stability is good, has a good application prospect.
The third object of the present invention is applied in providing application of the above-mentioned feed addictive in jewfish feed is prepared
In preparing jewfish feed, jewfish liver oxidation resistance can not only be improved, reduce endoplasmic reticulum oxidative damage, while can also reduced interior
Matter net stress protein is expressed, and alleviates er stress, moreover it is possible to improve intracellular Ca2+Stable state, so as to maintain the steady of endoplasmic reticulum
State.
The present invention is solved its technical problem and is realized using following technical scheme：
The present invention proposes a kind of feed addictive for alleviating jewfish er stress, the feed addictive of every 100 parts by weight
Alfalfa saponin including 20-30 parts by weight, the Methionine Chelated Zinc of 10-20 parts by weight, the taurine of 5-15 parts by weight, 20-30
The kudzu-vine root powder of parts by weight and the grape pip procyanidin of 5-10 parts by weight, surplus are carrier.
The present invention also proposes the preparation method of above-mentioned feed addictive：By proportioning mixing alfalfa saponin, Methionine Chelated Zinc,
Taurine, kudzu-vine root powder, grape pip procyanidin and carrier.
In addition, the application the invention also provides above-mentioned feed addictive in jewfish feed is prepared, such as can be by quality
Percentage is that the ratio of 0.5-1.5% is made an addition in jewfish feed.
Feed addictive of alleviation jewfish er stress that present pre-ferred embodiments provide and preparation method thereof is with answering
Advantageous effect is：
(1) raw material selected by is safe, without hazard residue, and condition is created for production green feed and aquatic products.
(2) product target for modulation is clear and definite, safe and efficient, formula is reasonable, has synergistic effect, compatible use ratio between each component
Single use effect is more preferable, more stable.
(3) product can improve jewfish liver oxidation resistance, reduce endoplasmic reticulum oxidative damage.
(4) product can improve intracellular Ca2+Stable state maintains the stable state of endoplasmic reticulum.
(5) product can inhibit the expression of er stress GAP-associated protein GAP, inhibit endoplasmic reticulum and excessively stress
(6) preparation method is simple, and condition controllability is good, and feed stability is good, has good application prospect.
(7) it is used to prepare jewfish feed and feeds jewfish, jewfish liver oxidation resistance can not only be improved, reduce endoplasmic reticulum
Oxidative damage, while binding protein expression can also be reduced, alleviate er stress, moreover it is possible to improve intracellular Ca2+
Stable state, so as to maintain the stable state of endoplasmic reticulum.
Description of the drawings
It in order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described.
Fig. 1 is normally to organize structure chart of the jewfish liver cell under transmission electron microscope in test example 4；
Fig. 2 is structure chart of the high in fat group of jewfish liver cell under transmission electron microscope in test example 4；
Fig. 3 is structure chart of the 3 jewfish liver cell of test group under transmission electron microscope in test example 4.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Below to feed addictive of alleviation jewfish er stress of the embodiment of the present invention and preparation method and application
It is specifically described.
It has been observed that endoplasmic reticulum is cell processing protein and storage Ca2+Main place, ATP exhausts, a large amount of free radicals
Generation and Ca2+Stable state destruction etc. can cause endoplasmic reticulum dysfunction, trigger er stress.
Therefore, fatty,fiss liver often occurs anti-stress ability reduces, cell oxidative damage and phenomena such as apoptosis all with it is interior
The dysfunction of matter net has extremely close relationship.
Based on above-mentioned discovery, the feed addictive provided in an embodiment of the present invention for alleviating jewfish er stress, every 100 weight
Measure the feed addictive of part can for example include the alfalfa saponins of 20-30 parts by weight, 10-20 parts by weight Methionine Chelated Zinc,
The grape pip procyanidin of the taurine of 5-15 parts by weight, the kudzu-vine root powder of 20-30 parts by weight and 5-10 parts by weight, surplus are
Carrier.
Preferably, the above-mentioned feed addictive of every 100 parts by weight for example can include 23-27 parts by weight alfalfa saponin,
The Methionine Chelated Zinc of 12-18 parts by weight, the taurine of 8-12 parts by weight, the kudzu-vine root powder of 22-28 parts by weight and 6-9 parts by weight
Grape pip procyanidin, surplus is carrier.
More preferably, the above-mentioned feed addictive of every 100 parts by weight can for example include alfalfa saponin, 15 weights of 25 parts by weight
The grape pip for measuring the Methionine Chelated Zinc of part, the taurine of 10 parts by weight, the kudzu-vine root powder of 25 parts by weight and 7.5 parts by weight is former
Anthocyanidin, surplus are carrier.
Optionally, above-mentioned carrier includes defatted rice bran and/or powdered rice hulls.
Wherein, alfalfa saponin is the active material with unique biological property extracted from clover.Specifically, clover
Saponin(e is the cyclic acetal object formed by the hydroxyl of hydroxyl in sugar or non-carbohydrates with acetal chain dehydrating condensation, and structure is
Pentacyclic triterpene alkenes compound.
In general, alfalfa saponin is used to treat gastrointestinal haemorrhagia, promotes enterogastric peristalsis and heat-clearing etc..In the embodiment of the present invention,
Alfalfa saponin is used to play anti-apoptotic, enhances activities of antioxidant enzymes in jewfish body, improves the intracellular Ca of jewfish2+The effects that stable state,
So as to protect jewfish endoplasmic reticulum, alleviate er stress.
It is mainly used for inhibiting binding protein in the feed addictive that Methionine Chelated Zinc is provided in the present invention program
Expression and activation activities of antioxidant enzymes.Zinc as one of important trace element, to maintain jewfish function of immune system and
Function of Antioxidant System, adjusting nutrient metabolism, adjusting DNA replication dna and nucleic acid synthesis are respectively provided with important function.
At present, in order to supplement the content of zinc, inorganic zinc salt or simple zinc salts of organic acid are typically supplemented.Above-mentioned inorganic acid
For zinc salt such as can be zinc sulfate, zinc chloride and zinc acetate, simple zinc salts of organic acid for example can be zinc lactate, glucose
Sour zinc and licorzinc etc..But inorganic zinc salt is digested and assimilated to jewfish with undesirable stimulation, and biological value is inclined
It is low；And though simple zinc salts of organic acid absorption efficiency is good compared with inorganic zinc, it synthesizes complicated, and yield and absorptivity are relatively low, fit
With being limited in scope.
In consideration of it, in the embodiment of the present invention by the way of Methionine Chelated Zinc, the zinc ion in chelate is made not only
Coordinate bond is formed with methionine, and ionic bond can also be formed with its carboxyl and form five yuan or hexatomic ring, so that zinc ion quilt
It is enclosed in the chela ring of chelate, shows good stability.Also, Methionine Chelated Zinc can by methionine to zinc from
The protective effect that rises of son protects it from the ill-effects such as hydrochloric acid in gastric juice in other ingredients and stomach and intestine in food, protects the physics and chemistry of zinc
Property, antagonism is few, improves the bioavailability of zinc.
In addition, containing methionine in Methionine Chelated Zinc, lipid peroxide can be avoided to damage primary and secondary lyase
Body film, the acid phosphatase as hydrolysis for causing to contain in lysosome release and to important thin such as cell and endoplasmic reticulum
Born of the same parents' device damages.In addition, methionine can also promote liver plasma membrane phospholipid methylation, enhance membrane fluidity, it is thin to reduce liver
The siltation of intracellular bile, reinforcement turn sulfenyl effect, reduce bile acid and are built up in liver, have preferable detoxication.
By using the Methionine Chelated Zinc simultaneously containing methionine and zinc, the work of methionine and zinc can not only be had both
With, and can also more individual methionine or zinc there is higher biological value.Therefore, the egg used in the embodiment of the present invention
Propylhomoserin chelated zinc not only reduces the dosage of zinc, also reduces production cost.
It is preferred that the ligand ratio (molar ratio of methionine and Zn-ef ficiency) in above-mentioned Methionine Chelated Zinc is preferably controlled in
3.5-4：1, the stability of Methionine Chelated Zinc is relatively suitble to jewfish to absorb in the range of this ligand ratio.Ligand can be caused than too small
The chelate of formation is unstable, and free zinc ion, which can be catalyzed, causes hydroxyl radical free radical to generate；Ligand is than excessive, though chelate is steady
Qualitative enhancing, but after its stability is too strong, absorbed not only bad for jewfish, while the waste of methionine can be also caused, from
And increase production cost.
It is preferred that in above-mentioned Methionine Chelated Zinc the chelation percent of Zn-ef ficiency preferably under 89-92%, this chelation percent zinc it is steady
It is qualitative relatively strong, it can not only avoid generating pessimal stimulation in jewfish body, while also be well suited to digesting and assimilating for jewfish.
Taurine is generally used to protect retina and promotes nervous centralis development, as feeding in the embodiment of the present invention
One of ingredient of feed additives is mainly used for the expression for the binding protein for playing antioxidation and inhibiting jewfish.This
Outside, taurine can also cooperate with the dissolubility for increasing lipid and cholesterol with Methionine Chelated Zinc, release cholestasia, reduce some
The cytotoxicity of free bile acid.
Kudzu-vine root powder commonly used in antipyretic relieving restlessness, promote the production of body fluid to quench thirst and blood pressure lowering, its in the embodiment of the present invention primarily serves anti-
Oxidation removes free radical, maintains intracellular Ca2+Concentration and alleviate er stress effect.
Grape pip procyanidin have superpower anti-oxidant efficiency, immunity can be improved, in embodiments of the present invention its
It is mainly used for inhibiting binding protein expression.
Above-mentioned raw materials ingredient also has synergistic function, example in addition to each having individual effect between each raw material
Such as：Alfalfa saponin can cooperate with anti-oxidant, removing free radical, the intracellular Ca of collaboration maintenance with kudzu-vine root powder2+Stable state inhibits endoplasmic reticulum
It stress.Methionine Chelated Zinc, taurine and grape pip procyanidin, which can cooperate with, inhibits binding protein expression.
From the above, after the raw material in the feed addictive of the embodiment of the present invention is used cooperatively, jewfish liver can not only be improved
Dirty oxidation resistance reduces endoplasmic reticulum oxidative damage, while can also reduce binding protein expression, and alleviating endoplasmic reticulum should
Swash, moreover it is possible to improve intracellular Ca2+Stable state, so as to maintain the stable state of endoplasmic reticulum.
In addition, the embodiment of the present invention additionally provides a kind of preparation method of above-mentioned feed addictive namely by said ratio
Mix alfalfa saponin, Methionine Chelated Zinc, taurine, kudzu-vine root powder, grape pip procyanidin and carrier.
In view of each raw material is different in the content of feed addictive and the property of each raw material is different, to enable each raw material uniform
It mixes, raw material can for example be mixed step by step in the following manner in the embodiment of the present invention：First grape pip procyanidin and part are carried
Body mixes, and is then mixed with taurine, then is mixed successively with kudzu-vine root powder and alfalfa saponin, obtains the first mixture, will be remaining
Carrier mixes after being mixed with Methionine Chelated Zinc with the first mixture again.
It is preferred that before combination, can all raw materials be subjected to ultramicro grinding, 80-100 mesh sieves then be crossed, to improve solid
The particle homogeneity of raw material improves the edibility and digestibility of feed addictive.
In addition, the embodiment of the present invention additionally provides a kind of application of above-mentioned feed addictive, such as can use it for preparing
Jewfish feed simultaneously feeds jewfish, so as to effectively improve jewfish liver oxidation resistance, reduce endoplasmic reticulum oxidative damage, reduces
Binding protein is expressed, and alleviates er stress, maintains the stable state of endoplasmic reticulum.
Optionally, which for example can make an addition to jewfish feed for 0.5-1.5% by weight percent
In, such as can be added in by 0.5wt%, 1wt% and 1.5wt% in jewfish feed, preferably added in by 1wt% to jewfish feed
In.Under the adding proportion, the cost performance of feed addictive is optimal.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
Before mixing, all raw materials are subjected to ultramicro grinding and cross 80 mesh sieves, are contained by the feed addictive of every 100 parts by weight
The alfalfa saponin of 20 parts by weight, the Methionine Chelated Zinc of 10 parts by weight, the taurine of 5 parts by weight, 20 parts by weight kudzu-vine root powder with
And 5 parts by weight grape pip procyanidin, surplus mixes each raw material for the ratio of defatted rice bran, must alleviate jewfish endoplasm
Net stress feed addictive.
Wherein, the ligand ratio of Methionine Chelated Zinc is 3.5：1, the chelation percent of Zn-ef ficiency is 89% in Methionine Chelated Zinc.
Embodiment 2
The present embodiment and embodiment 1 difference lies in：Before mixing, all raw materials are subjected to ultramicro grinding and cross 100 mesh sieves,
Contain the alfalfa saponin of 30 parts by weight, the Methionine Chelated Zinc of 20 parts by weight, 15 weight by the feed addictive of every 100 parts by weight
The taurine, the kudzu-vine root powder of 30 parts by weight and the grape pip procyanidin of 10 parts by weight of part, surplus are the ratio of defatted rice bran
Each raw material is mixed, the feed addictive of jewfish er stress must be alleviated.
Embodiment 3
The present embodiment and embodiment 1 difference lies in：Before mixing, all raw materials are subjected to ultramicro grinding and cross 90 mesh sieves,
Contain the alfalfa saponin of 23 parts by weight, the Methionine Chelated Zinc of 12 parts by weight, 8 weight by the feed addictive of every 100 parts by weight
The taurine, the kudzu-vine root powder of 22 parts by weight and the grape pip procyanidin of 6 parts by weight of part, surplus will for the ratio of defatted rice bran
Each raw material is mixed, and must alleviate the feed addictive of jewfish er stress.
Embodiment 4
The present embodiment and embodiment 1 difference lies in：Contain the lucerne of 27 parts by weight by the feed addictive of every 100 parts by weight
Mu saponin(e, the Methionine Chelated Zinc of 18 parts by weight, the taurine of 12 parts by weight, the kudzu-vine root powder of 28 parts by weight and 9 parts by weight
Grape pip procyanidin, surplus mix each raw material for the ratio of defatted rice bran, must alleviate the feeding of jewfish er stress
Feed additives.
Embodiment 5
The present embodiment and embodiment 1 difference lies in：Contain the lucerne of 25 parts by weight by the feed addictive of every 100 parts by weight
Mu saponin(e, the Methionine Chelated Zinc of 15 parts by weight, the taurine of 10 parts by weight, the kudzu-vine root powder of 25 parts by weight and 7.5 parts by weight
Grape pip procyanidin, surplus mixes each raw material for the ratio of defatted rice bran, must alleviate jewfish er stress
Feed addictive.
Embodiment 6
The present embodiment and embodiment 1 difference lies in：Ligand ratio in Methionine Chelated Zinc is 4： 1.
Embodiment 7
The present embodiment and embodiment 1 difference lies in：Ligand ratio in Methionine Chelated Zinc is 3.75：1.
Embodiment 8
The present embodiment and embodiment 1 difference lies in：The chelation percent of Zn-ef ficiency is 92% in Methionine Chelated Zinc.
Embodiment 9
The present embodiment and embodiment 1 difference lies in：The chelation percent of Zn-ef ficiency is 90% in Methionine Chelated Zinc.
Embodiment 10
The present embodiment and embodiment 1 difference lies in：Carrier is powdered rice hulls.
Embodiment 11
The present embodiment and embodiment 1 difference lies in：Carrier is defatted rice bran and the mixture of powdered rice hulls.
Embodiment 12
The present embodiment and embodiment 1 difference lies in：In mixed process, first grape pip procyanidin and Component Vectors are mixed
It closes, is then mixed with taurine, then mixed successively with kudzu-vine root powder and alfalfa saponin, the first mixture is obtained, by remaining carrier
It is mixed again with the first mixture after being mixed with Methionine Chelated Zinc.
Embodiment 13
The present embodiment provides it is a kind of alleviate jewfish er stress feed addictive application, by it by weight percent
It is made an addition to for 0.5% in jewfish feed.
Embodiment 14
The present embodiment provides it is a kind of alleviate jewfish er stress feed addictive application, by it by weight percent
It is made an addition to for 1% in jewfish feed.
Embodiment 15
The present embodiment provides it is a kind of alleviate jewfish er stress feed addictive application, by it by weight percent
It is made an addition to for 1.5% in jewfish feed.
Test example
It repeats to implement above-described embodiment 1-12, obtains the feed addictive of enough alleviation jewfish er stress.
It as test group 1-3 by taking embodiment 1,3,5 as an example, is designed using single-factor, by test fish domestication after a week, selected
Physically fit, specification homogeneous (counterpoise is 200 ± 3g) 1500 tail of jewfish, is randomly divided into 5 groups of (normal group, high in fat group, test groups
1-3), every group of 3 repetitions, it is each to repeat 100 tails.Jewfish cultivate in 15 seawater cages (specification for 2.0m × 2.0m ×
Cultivation in 2.0m).During culture experiment, feed daily 2 times (7:30 and 17:30), it is satiated with food and feeds, the culture-cycle is 10 weeks.Often
Group feed formula is as shown in table 1, normal group feed fat content 11%, high in fat group：Horizontal feed fat is 20% (this laboratory
Early-stage study shows：20% fat can cause er stress), test group：Horizontal feed fat is 20%, and adds this implementation
Additive in example.The particle that grain size is 5mm or so is machined to small particle, room temperature, which air-dries, is placed on 4 DEG C of refrigerators preservations
It is spare.
Culture experiment terminates, and fasting is for 24 hours.Count the mantissa of fish in each net cage.The MS- for being 100mg/L with concentration by fish
222 (Sigma, the U.S.) anaesthetize, and are placed on ice pan and gather liver, for testing following index.Result of the test such as table 2 is to 3 institute of table
Show.
1 each group feed formula group % of table
Note：Vitamin premix (mg or g/kg feeds) is respectively containing thiamine 25mg, riboflavin 45mg, hydrochloric acid in table 1
Pyridoxol 20mg, vitamin B120.1mg, vitamin K310mg, inositol 800mg, pantothenic acid 60mg, niacin 200mg, folic acid 20mg,
Biotin 1.2mg, retinyl acetate 32mg, vitamin D35mg, alpha-tocopherol 120mg, ethoxyquinoline 150mg, choline
(50%) 5000mg.
Mineral substance premix (mg/kg feeds) is respectively containing magnesium sulfate monohydrate 4000mg, monohydrate zinc sulphate 50mg, potassium iodide
(1%) 100mg, cobalt chloride (1%) 100mg, five water zinc sulphate 20mg, ferrous sulfate monohydrate 260mg, monohydrate zinc sulphate
150mg, sodium selenite (1%) 50mg.
The influence that 2 different disposal group of table expresses jewfish liver endoplasmic reticulum stress protein
| Gene relative expression quantity | Normal group | High in fat group | Test group 1 | Test group 2 | Test group 3 |
| GRP78 | 1.00c | 5.98a | 2.12b | 2.01b | 1.92b |
| CRT | 1.00c | 7.62a | 2.69b | 3.69b | 2.57b |
| ATF-6α | 1.00c | 4.21a | 1.25bc | 1.59b | 1.39b |
| Caspase 12 | 1.00c | 3.79a | 1.58b | 1.67b | 1.49b |
Note：Colleague's subscript does not have same letter to represent significant difference (P<0.05).
As can be seen from Table 2, after the feed addictive that addition embodiment 1,3,5 is provided, the endoplasm of the jewfish of test group
More high in fat group of the expression of net stress protein (gene GRP78, CRT, ATF-6 α and Caspase 12) significantly reduces (P<
0.05) binding protein expression can be significantly reduced by, showing to add feed addictive provided in an embodiment of the present invention, in alleviation
Matter net stress.The effect of test group 3 compared with test group 1 and test group 2 more preferably, illustrates test group it can be seen from test group 1-3
3 raw material proportioning is more conducive to reduce the binding protein expression of jewfish.
3 different disposal group endoplasmic reticulum oxidative damage parameters of table
Note：Colleague's subscript does not have same letter to represent significant difference (P<0.05).
As can be seen from Table 3, after the feed addictive that addition embodiment 1,3,5 is provided, total super oxygen of test group jewfish
More high in fat group of compound mutase SOD activity significantly raises (P<0.05) feed addictive tool provided in an embodiment of the present invention, is shown
Play the role of enhancing anti-oxidation function.In addition, after the feed addictive that addition embodiment 1,3,5 is provided, test group jewfish
Lipid peroxide MDA and more high in fat group of intracellular ROS contents significantly reduce (P<0.05), and with normal group without significance difference
It is different, show that feed addictive provided in an embodiment of the present invention can reduce the oxidative damage degree that endoplasmic reticulum is subjected to.By test group 1-3
As can be seen that the effect of test group 3 is compared with test group 1 and test group 2 more preferably, illustrate that the raw material proportioning of test group 3 is more conducive to carry
The total number born SOD activity of high jewfish, the lipid peroxide MDA for reducing jewfish and intracellular ROS contents.
According to the method for above-mentioned test example to the feed for alleviating jewfish er stress obtained by 2,4 and 6-12 of embodiment
Additive is tested, and result equally display contains alleviation jewfish er stress provided in an embodiment of the present invention by feeding
Feed addictive jewfish feed, can more high in fat group of reduction binding protein (gene GRP78, CRT, ATF-6 α with
And Caspase 12) expression, improve total number born SOD activity, reduce lipid peroxide MDA and intracellular
ROS contents.
And the resultant effect that wherein, embodiment 4 is risen compared with embodiment 2 is more preferably, comparative example 1-5, and embodiment 5 is relatively real
Apply a 1-4 it is more excellent to the resultant effect of jewfish endoplasmic reticulum and implement 3-4 it is more excellent compared with embodiment 1-2.
In addition, embodiment 12 compared with embodiment 5 to reduce binding protein (gene GRP78, CRT, ATF-6 α with
And Caspase 12) expression, improve total number born SOD activity, reduce lipid peroxide MDA and intracellular
ROS contents it is better, illustrate in preparation process, by raw material substep mixing relatively simultaneously mixing be more advantageous to maintain endoplasmic reticulum
Structure and improve its function.
Test example 2
By the test method of test example 1, control group 1-4 is set, and control group 1-4 and the difference of embodiment 5 are respectively：
Ligand ratio in control group 1 in Methionine Chelated Zinc is 1:1, the ligand ratio in control group 2 in Methionine Chelated Zinc is 2:1, it is right
It is 5 according to the ligand ratio in Methionine Chelated Zinc in group 3:1, the ligand ratio in control group 4 in Methionine Chelated Zinc is 6:1.To reality
It applies a 5-7 and control group 1-4 is measured, the results show：
More high in fat group is compared, embodiment 5-7 compared with control group 1-4 binding protein (gene GRP78, CRT,
ATF-6 α and Caspase 12) expression reduction is more notable, and the reduction degree of embodiment 5 is most notable.In addition, embodiment 5-
7 are improving total number born SOD activity and are reducing lipid peroxide MDA and intracellular compared with control group 1-4
ROS contents are more notable, and the improvement degree of embodiment 5 is most notable.
Test example 3
By the test method of test example 1, control group 5-7 is set, and control group 5-7 and the difference of embodiment 5 are respectively：
The chelation percent of Zn-ef ficiency is 80% in Methionine Chelated Zinc in control group 5, Zn-ef ficiency in Methionine Chelated Zinc in control group 6
Chelation percent is 85%, and the chelation percent of Zn-ef ficiency is 98% in Methionine Chelated Zinc in control group 7.To embodiment 5,8,9 and right
It is measured according to a group 5-7, the results show：
More high in fat group is compared, embodiment 5,8,9 compared with control group 5-7 binding protein (gene GRP78,
CRT, ATF-6 α and Caspase 12) expression reduction is more notable, and the reduction degree of embodiment 5 is most notable.In addition, implement
Example 5,8,9 compared with control group 5-7 improve total number born SOD activity and reduce lipid peroxide MDA and
Intracellular ROS contents are more notable, and the improvement degree of embodiment 5 is most notable.
Test example 4
By the test group 3 at the end of experimental period in test example 1 and the liver cell of normal group and high in fat group of jewfish in saturating
Radio Microscopic observation, result is as shown in Figs. 1-3.It can be seen that by Fig. 1-3：Endoplasmic reticulum arrangement in the liver cell of normal group jewfish
Bound ribosome in rule, endoplasmic reticulum, matrix is uniform, and film is clear.Endoplasmic reticulum irregular arrangement, the endoplasmic reticulum of high in fat group of jewfish swell
It is swollen serious and be broken, ribosomes comes off.More high in fat group of test group can recover endoplasmic reticulum ultra microstructure, alleviate the swollen of endoplasmic reticulum
It is swollen.Thus illustrate, by the jewfish for feeding the feed addictive containing alleviation jewfish er stress provided in an embodiment of the present invention
Feed is conducive to maintain the ultra microstructure of jewfish endoplasmic reticulum complete.
In conclusion the raw material of the feed addictive provided in an embodiment of the present invention for alleviating jewfish er stress is pacified
Entirely, without hazard residue, condition is created for production green feed and aquatic products.Its preparation method is simple, and condition controllability is good, surely
It is qualitative good, it has a good application prospect.Above-mentioned feed addictive is applied to prepare jewfish feed, jewfish liver can not only be improved
Dirty oxidation resistance reduces endoplasmic reticulum oxidative damage, while can also reduce binding protein expression, and alleviating endoplasmic reticulum should
Swash, moreover it is possible to improve intracellular Ca2+Stable state, so as to maintain the stable state of endoplasmic reticulum.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
- A kind of 1. feed addictive for alleviating jewfish er stress, which is characterized in that the feed addition of every 100 parts by weight Agent includes the alfalfa saponin of 20-30 parts by weight, the Methionine Chelated Zinc of 10-20 parts by weight, taurine, the 20- of 5-15 parts by weight The kudzu-vine root powder of 30 parts by weight and the grape pip procyanidin of 5-10 parts by weight, surplus are carrier.
- 2. feed addictive according to claim 1, which is characterized in that the feed addictive bag of every 100 parts by weight Include the alfalfa saponin of 23-27 parts by weight, the Methionine Chelated Zinc of 12-18 parts by weight, the ox of 8-12 parts by weight The grape pip procyanidin of sulfonic acid, the kudzu-vine root powder of 22-28 parts by weight and 6-9 parts by weight, surplus are the carrier.
- 3. feed addictive according to claim 2, which is characterized in that the feed addictive bag of every 100 parts by weight Include the alfalfa saponin of 25 parts by weight, the Methionine Chelated Zinc of 15 parts by weight, the taurine, 25 weights of 10 parts by weight The kudzu-vine root powder of part and the grape pip procyanidin of 7.5 parts by weight are measured, surplus is the carrier.
- 4. according to claim 1-3 any one of them feed addictives, which is characterized in that matching somebody with somebody in the Methionine Chelated Zinc Body ratio is 3.5-4：1.
- 5. according to claim 1-3 any one of them feed addictives, which is characterized in that zinc member in the Methionine Chelated Zinc The chelation percent of element is 89-92%.
- 6. according to claim 1-3 any one of them feed addictives, which is characterized in that the carrier includes defatted rice bran And/or powdered rice hulls.
- 7. such as the preparation method of claim 1-6 any one of them feed addictives, which is characterized in that as described in proportioning mixing Alfalfa saponin, the Methionine Chelated Zinc, the taurine, the kudzu-vine root powder, the grape pip procyanidin and the load Body.
- 8. preparation method according to claim 7, which is characterized in that first will be described in the grape pip procyanidin and part Carrier mixes, and is then mixed with the taurine, then is mixed successively with the kudzu-vine root powder and the alfalfa saponin, and it is mixed to obtain first Object is closed, is mixed again with first mixture after the remaining carrier is mixed with the Methionine Chelated Zinc.
- 9. preparation method according to claim 8, which is characterized in that before mixing, all raw materials are subjected to ultramicro grinding simultaneously Cross 80-100 mesh sieves.
- 10. such as application of the claim 1-6 any one of them feed addictive in jewfish feed is prepared, which is characterized in that The feed addictive is made an addition to for the ratio of 0.5-1.5% in jewfish feed by weight percent；Preferably, the feed addictive is made an addition to for 1% ratio in jewfish feed by weight percent.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367378A (en) * | 2019-09-02 | 2019-10-25 | 集美大学 | Feed addictive, preparation method, application and feed |
| CN110810673A (en) * | 2019-12-11 | 2020-02-21 | 集美大学 | Feed additive for reducing body fat deposition of sea bass |
| CN113995059A (en) * | 2021-10-11 | 2022-02-01 | 珠海市德海生物科技有限公司 | Feed additive for improving anti-stress capability of sea bass and preparation method thereof |
| CN116784438A (en) * | 2023-07-10 | 2023-09-22 | 佛山市顺德区旺海饲料实业有限公司 | Feed for preventing soybean meal from inducing damage to endoplasmic reticulum of tilapia and preparation method of feed |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1823880A (en) * | 2005-12-28 | 2006-08-30 | 苏云驰 | Preparation method of medicine or food for thinning reducing fat |
| CN101491300A (en) * | 2009-03-05 | 2009-07-29 | 浙江大学 | Feed additive capable of preventing and treating large yellow croaker fatty liver |
| CN102125539A (en) * | 2010-12-17 | 2011-07-20 | 中国水产科学研究院淡水渔业研究中心 | Liver protection and bile secretion promotion preparation for fish and preparation method thereof |
| CN102210411A (en) * | 2011-06-08 | 2011-10-12 | 广东恒兴饲料实业股份有限公司 | Compound feed capable of improving fatty liver and body colour of marine fish and preparation method thereof |
| CN105394330A (en) * | 2015-10-28 | 2016-03-16 | 湛江恒兴珊瑚饲料有限公司 | Lateolabrax japonicus puffed feed and preparation method thereof |
| CN106036231A (en) * | 2016-06-28 | 2016-10-26 | 甄建民 | Fish feed capable of preventing and treating crucian nutritional type fatty liver and preparation method of fish feed |
| CN106805003A (en) * | 2016-12-13 | 2017-06-09 | 浙江大学舟山海洋研究中心 | A kind of higher fatty acid black porgy feed |
| CN106901073A (en) * | 2017-03-29 | 2017-06-30 | 广东天邦饲料科技有限公司 | A kind of expanded slow heavy mixed feed of largemouth bass well doneization |
-
2018
- 2018-02-12 CN CN201810147688.4A patent/CN108077585B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1823880A (en) * | 2005-12-28 | 2006-08-30 | 苏云驰 | Preparation method of medicine or food for thinning reducing fat |
| CN101491300A (en) * | 2009-03-05 | 2009-07-29 | 浙江大学 | Feed additive capable of preventing and treating large yellow croaker fatty liver |
| CN102125539A (en) * | 2010-12-17 | 2011-07-20 | 中国水产科学研究院淡水渔业研究中心 | Liver protection and bile secretion promotion preparation for fish and preparation method thereof |
| CN102210411A (en) * | 2011-06-08 | 2011-10-12 | 广东恒兴饲料实业股份有限公司 | Compound feed capable of improving fatty liver and body colour of marine fish and preparation method thereof |
| CN105394330A (en) * | 2015-10-28 | 2016-03-16 | 湛江恒兴珊瑚饲料有限公司 | Lateolabrax japonicus puffed feed and preparation method thereof |
| CN106036231A (en) * | 2016-06-28 | 2016-10-26 | 甄建民 | Fish feed capable of preventing and treating crucian nutritional type fatty liver and preparation method of fish feed |
| CN106805003A (en) * | 2016-12-13 | 2017-06-09 | 浙江大学舟山海洋研究中心 | A kind of higher fatty acid black porgy feed |
| CN106901073A (en) * | 2017-03-29 | 2017-06-30 | 广东天邦饲料科技有限公司 | A kind of expanded slow heavy mixed feed of largemouth bass well doneization |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367378A (en) * | 2019-09-02 | 2019-10-25 | 集美大学 | Feed addictive, preparation method, application and feed |
| CN110367378B (en) * | 2019-09-02 | 2022-04-12 | 集美大学 | Feed additive, preparation method and application thereof and feed |
| CN110810673A (en) * | 2019-12-11 | 2020-02-21 | 集美大学 | Feed additive for reducing body fat deposition of sea bass |
| CN113995059A (en) * | 2021-10-11 | 2022-02-01 | 珠海市德海生物科技有限公司 | Feed additive for improving anti-stress capability of sea bass and preparation method thereof |
| CN116784438A (en) * | 2023-07-10 | 2023-09-22 | 佛山市顺德区旺海饲料实业有限公司 | Feed for preventing soybean meal from inducing damage to endoplasmic reticulum of tilapia and preparation method of feed |
| CN116784438B (en) * | 2023-07-10 | 2023-12-26 | 佛山市顺德区旺海饲料实业有限公司 | Feed for preventing soybean meal from inducing damage to endoplasmic reticulum of tilapia and preparation method of feed |
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| CN108077585B (en) | 2019-07-26 |
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