CN108072730A - A kind of TLC Identification of high eyebrow hymsleya amabilis - Google Patents
A kind of TLC Identification of high eyebrow hymsleya amabilis Download PDFInfo
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Abstract
The invention discloses a kind of TLC Identifications of high eyebrow hymsleya amabilis, comprise the following steps：(1) preparation of test solution：Medicinal powder to be checked is taken, methanol extraction is added in, filters, test solution is prepared in concentration, methanol constant volume；(2) preparation of reference substance solution：Reference substance solution is prepared by reference substance of Hemsleyadin first；(3) thin layer differentiates：By reference substance solution and test solution, point sample, solvent expansion, colour developing are inspected, comparison on lamellae respectively；The solvent is by following parts by volume into being grouped into：90~95 parts by volume of chloroform, 5~10 parts by volume of ethyl acetate, 5~10 parts by volume of methanol.The high eyebrow hymsleya amabilis TLC Identification of the present invention, suitable reference substance and unfolding condition are chosen, the target component of high eyebrow hymsleya amabilis is effectively separated, it can accurately and reliably differentiate high eyebrow hymsleya amabilis, itself and dolichocarpel hemsleya root and Pengxian County hymsleya amabilis are distinguished, effective guarantee is provided for clinical application.
Description
Technical field
The present invention relates to medicinal material detection fields, and in particular to a kind of TLC Identification of high eyebrow hymsleya amabilis.
Background technology
It is Curcurbitaceae hymsleya amabilis platymiscium dolichocarpel hemsleya root Hemsleya dolichocarpa that recorded hymsleya amabilis is marked in river
The colored hymsleya amabilis Hemsleya of W.J.Chang, high eyebrow hymsleya amabilis Hems-leya omeiensis L.T.Shen et W.J.Chang or huge
The dried root of gigantha W.J.Chang.Saponin(e containing hymsleya amabilis, Hemsleyadin.Excavation at the beginning of last month of spring in autumn, removes impurity, takes advantage of fresh-cut
Piece, it is dry.Alias cockchafer lotus, golden basin, arched bottom, Sichuan Province, which is practised, claims cockchafer lotus.It can clearing heat and detoxicating, anti-inflammatory analgetic.For bacillary dysentery,
Enteritis, bronchitis, acute tonsillitis, toothache caused by fire of deficiency type, stomachache.This product history tree is on the books.《Book on Chinese herbal medicine is just square》Claim gold
Basin,《Revision and Addition Tian Bao book on Chinese herbal medicine》With《Traditional Chinese Medicine in Sichuan will》Claim cockchafer lotus,《Classify herbal》It is recorded with gold and silver basin.
Wherein, dolichocarpel hemsleya root taste is extremely bitter, Pengxian County hymsleya amabilis slight bitter, and high eyebrow hymsleya amabilis taste is not bitter.Therefore, compared with other two kind phases
Than the characteristics of high eyebrow hymsleya amabilis taste is not bitter, which is more easy to be received by patient, is suitable for clinic to be taken.High eyebrow hymsleya amabilis is good to treatment gastropyretic toothache
Good effect makes it by market wilderness demand.
However, it is less for the research of high eyebrow hymsleya amabilis at present, it is more rare to the composition Study of high eyebrow hymsleya amabilis, it is many effective
Ingredient or characteristic chemical constituent are unknown, so as to lack quantifiable quality evaluating method and standard to it.Also, high eyebrow snow
Courage is also difficult to kind such as dolichocarpel hemsleya root approximate with its etc. and differentiation is effectively performed, especially dried medicinal material.
Therefore, in order to ensure the quality of high eyebrow hymsleya amabilis and safely, it is necessary to a kind of side that can accurately, reliably differentiate high eyebrow hymsleya amabilis
Method.
The content of the invention
To solve the above problems, the present invention provides a kind of TLC Identification of high eyebrow hymsleya amabilis, including following step
Suddenly：
(1) preparation of test solution：
Medicinal powder to be checked is taken, methanol extraction is added in, filters, test solution is prepared in concentration, methanol constant volume；
(2) preparation of reference substance solution：
Reference substance solution is prepared by reference substance of Hemsleyadin first；
(3) thin layer differentiates：
By reference substance solution and test solution, point sample, solvent expansion, colour developing are inspected, compared on lamellae respectively
；The solvent is by following parts by volume into being grouped into：
90~95 parts by volume of chloroform, 5~10 parts by volume of ethyl acetate, 5~10 parts by volume of methanol.
Further, the solvent by following parts by volume into being grouped into：
90 parts by volume of chloroform, 10 parts by volume of ethyl acetate, 10 parts by volume of methanol.
Further, in step (1), the extraction is refluxing extraction.
Further, when the time of the refluxing extraction is 1.5 small.
Further, in the methanol extraction of the step (1), the envelope-bulk to weight ratio of methanol and medicinal material to be checked is 10mL:1g.
Further, the envelope-bulk to weight ratio of the methanol constant volume volume of the step (1) and medicinal material to be checked is 2mL：1g.
Further, the reference substance solution of the step (2) is the methanol solution of Hemsleyadin first；Preferably, Hemsleyadin first
Concentration be 2mg/mL.
Further, in step (3), the point sample amount of the reference substance solution point sample is 2 μ L, the point of test solution point sample
Sample amount is 5 μ L.
Further, in step (3), the colour developing is using 10% phosphomolybdic acid ethanol solution as color developing agent, is heated at 120 DEG C
Colour developing.
Further, it is described to compare as following step A or/and B in step (3)：
Step A, in test sample chromatography, on position corresponding with Hemsleyadin first reference substance chromatography, if aobvious same color
Spot；
Step B, in test sample chromatography, if there are Rf values for 0.84 ± 0.03,0.61 ± 0.05,0.50 ± 0.04,
0.28 ± 0.05,0.16 ± 0.04 blueness/pewter spot.
Further, the discriminating is to discriminate between high eyebrow hymsleya amabilis and dolichocarpel hemsleya root or Pengxian County hymsleya amabilis.
The high eyebrow hymsleya amabilis TLC Identification of the present invention, has chosen suitable reference substance and unfolding condition, will be high
The target component of eyebrow hymsleya amabilis is effectively separated, and can accurately and reliably differentiate high eyebrow hymsleya amabilis, by itself and dolichocarpel hemsleya root and Pengxian County
Hymsleya amabilis distinguishes, and effective guarantee is provided for clinical application.
Obviously, the above according to the present invention according to the ordinary technical knowledge and customary means of this field, is not departing from
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific embodiment of form by the following examples remakes further specifically the above of the present invention
It is bright.But the scope that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following example.It is all to be based on the above of the present invention
The technology realized all belongs to the scope of the present invention.
Description of the drawings
Fig. 1 is the thin-layer chromatogram of medicinal material and reference substance to be checked under 1 testing conditions of embodiment：1st, 5 compareed for Hemsleyadin first
Product, 2-4 are the high eyebrow hymsleya amabilis sample of different batches；6 be dolichocarpel hemsleya root sample.
Fig. 2 is the thin-layer chromatogram of medicinal material and reference substance to be checked under 1 testing conditions of embodiment：D compares for Hemsleyadin first
Product；1-3 is the high eyebrow hymsleya amabilis sample of different batches；4 be Pengxian County hymsleya amabilis sample；5 be dolichocarpel hemsleya root sample.
Fig. 3 is the thin-layer chromatogram of medicinal material and reference substance to be checked under comparative example testing conditions：D:Reference substance；1-3 is difference
The high eyebrow hymsleya amabilis sample of batch；4 be Pengxian County hymsleya amabilis sample.
Fig. 4 is investigated for extracting method under item, the thin-layer chromatogram of medicinal material and reference substance to be checked：D:Reference substance；1:Methanol returns
Flow 1.5h；2:Methanol ultrasound 0.5h；3:Alcohol reflux 1.5h；4:Methanol eddy 1h；5:Alcohol reflux 1h.
Fig. 5 is investigated for color developing agent under item, when 5% phosphomolybdic acid ethyl alcohol is as color developing agent, the thin layer of medicinal material and reference substance to be checked
Chromatogram：D:Reference substance；1-3:High eyebrow hymsleya amabilis；4:Dolichocarpel hemsleya root；5:Pengxian County hymsleya amabilis.
Fig. 6 is investigated for color developing agent under item, when 10% phosphomolybdic acid ethyl alcohol is as color developing agent, the thin layer of medicinal material and reference substance to be checked
Chromatogram：D:Reference substance；1-3:High eyebrow hymsleya amabilis；4:Dolichocarpel hemsleya root；5:Pengxian County hymsleya amabilis.
Fig. 7 is investigated for point sample amount under item, the thin-layer chromatogram of medicinal material and reference substance to be checked on prefabricated board：1st, 2,3,8 points
It is not that reference substance point sample amount is investigated, point sample volume is 1,2,5,3 μ L respectively；4-7 is the investigation of medicinal material point sample amount to be checked respectively, point
Sample volume integral ratio is 1,2,5,3 μ L.
Fig. 8 is investigated for point sample amount under item, the thin-layer chromatogram of medicinal material and reference substance to be checked on self-control silica gel plate：D1-D3Point
It is not the point sample amount investigation of reference substance, point sample volume is 2,4,5 μ L respectively；1-5 is the investigation of sample point sample amount respectively, point sample body
Integration is not 2,4,5,7,10 μ L.
Fig. 9 is chloroform under the investigation item of solvent：Ethyl alcohol=(95:5) when being solvent, medicinal material to be checked and control
The thin-layer chromatogram of product：D:Reference substance；1-4:High eyebrow hymsleya amabilis.
Figure 10 is chloroform under the investigation item of solvent：Methanol：Ethyl acetate=(90:10:10), medicinal material to be checked and
The thin-layer chromatogram of reference substance：D:Reference substance；1-5:High eyebrow hymsleya amabilis.
Specific embodiment
Experiment material used in following embodiments is as follows：
Material and reagent：Hemsleyadin first reference substance (purity >=98.0%, lot number：16061504, Chengdu Fei Pude biology skills
Art Co., Ltd)；Methanol, chloroform, ethyl acetate, absolute ethyl alcohol are that analysis is pure.
High eyebrow hymsleya amabilis is the high eyebrow hymsleya amabilis Hemsleya omeiensis L.T.Shen et W.J.Chang of cucurbitaceous plant
Dried root, harvest in Mount Emei of Sichuan Province.
Dolichocarpel hemsleya root is the dried pieces of cucurbitaceous plant dolichocarpel hemsleya root Hemsleya dolichocarpa W.J.Chang
Root is harvested in Sichuan Province Pengzhou.
Pengxian County hymsleya amabilis is the dried pieces of cucurbitaceous plant Pengxian County hymsleya amabilis Hemsleya pengxianensis W.J.Chang
Root is harvested in Sichuan Province Pengzhou.
The TLC Identification of 1 present invention of embodiment
(1) preparation of test solution
Medicinal powder 2g to be checked is taken, adds 10 times of amount (20mL) methanol condensed reflux 1.5h, filtration, filtrate water-bath concentration adds
Methanol constant volume is to 4mL, as test solution.
(2) preparation of reference substance solution
Hemsleyadin first reference substance is taken, solution of every 1mL containing 2mg is made with methanol, as reference substance solution.
(3) indentification by TLC
According to thin-layered chromatography (《Chinese Pharmacopoeia》The 4th general rule 0502 of version in 2015) experiment, it is molten that test sample is drawn respectively
Liquid, reference substance solution 5ul are put respectively on same silica gel g thin-layer plate, with chloroform-acetate-methanol=90:10:10
For solvent, it is unfolded, takes out, dry, spray with 10% phosphomolybdic acid ethanol solution, clear spot is heated at 120 DEG C, takes out,
It inspects, as a result as depicted in figs. 1 and 2.
It will be seen from figure 1 that clear spot is as it can be seen that separating degree is good.In test sample chromatography, with Hemsleyadin first reference substance
On (Rf values are 0.538) corresponding position of chromatography, the spot of same color is very shallow to be practically free of.High eyebrow hymsleya amabilis can clearly be seen that
The principal spot of 5 blueness~dusty blue, Rf values are 0.846,0.615,0.505,0.286,0.165 respectively from top to bottom.
And dolichocarpel hemsleya root is avenged in the spot identical with occurring color in reference substance cucurbitacin same position with high eyebrow
The equal very slight color of spot that 5 principal spot same positions of courage occur, thus can using this as the high eyebrow hymsleya amabilis of differentiation it is important according to
According to, and high eyebrow hymsleya amabilis is in the spot with being practically free of same color in reference substance same position, it can be effective by the method
Identify high eyebrow hymsleya amabilis.
Figure it is seen that high eyebrow hymsleya amabilis can clearly be seen that the principal spot of 5 blueness~dusty blue, Rf from top to bottom
Value is 0.846,0.615,0.505,0.286,0.165 respectively.
And dolichocarpel hemsleya root is avenged in the spot identical with occurring color in reference substance cucurbitacin same position with high eyebrow
The equal very slight color of spot that 5 principal spot same positions of courage occur, thus can using this as the high eyebrow hymsleya amabilis of differentiation it is important according to
According to can effectively identify high eyebrow hymsleya amabilis by the method.
Pengxian County hymsleya amabilis is very shallow in the same color spot with occurring in reference substance cucurbitacin same position, illustrates hardly
Contain or less containing cucurbitacin, and the thin-layer chromatography of Pengxian County hymsleya amabilis has the very deep background colour of a color, can substantially with high eyebrow
Hymsleya amabilis and dolichocarpel hemsleya root distinguish.
Comparative example
1987 conditions are marked according to document river, it is specific as follows：
Take this product powder 2g, add ethyl alcohol 20mL, put be heated to reflux in water-bath 1 it is small when, filtration, filtrate puts and is concentrated into water-bath
About 5mL makees test solution.Hemsleyadin first reference substance separately is taken, adds ethyl alcohol dissolving that solution of every 1mL containing 1mg is made, as control
Solution.According to thin layer chromatography (《Chinese Pharmacopoeia version in 1985》One annex page 37) experiment, draw 10 μ L of test solution, control
5 μ L of product solution are put respectively on same silica gel g thin-layer plate, with chloroform-ethanol (95；5) it is solvent, is unfolded, takes out, dry,
5% phosphomolybdic acid ethanol solution is sprayed, 120 DEG C is put and dries about 10 minutes.It takes out, inspects, the results are shown in Figure 3.
Fig. 3's the results show that the development system under the conditions of bibliography is unfolded, and the Rf values of reference substance are smaller, target blob
It is be unable to do without with other spots point, it is impossible to make apparent differentiation.
The investigation of 2 the method for the present invention process conditions of embodiment
Under the conditions of the thin layer of embodiment 1, using single factor exploration method to extracting method, color developing agent, point sample amount and expansion
Agent is investigated.
1st, the investigation of extracting method
Investigation condition is as follows：
1:Methanol eddy 1.5h；2:Methanol ultrasound 0.5h；3:Alcohol reflux 1.5h；4:Methanol eddy 1h；5:Alcohol reflux
1h.As shown in Figure 4.
It investigates the results show that the extraction effect of reflux is apparently higher than ultrasonic extraction, the spot colors of reflux are apparent, and spot is clear
It is clear；Methanol eddy extraction effect is substantially better than the effect of alcohol reflux extraction, under identical extraction time, under methanol extraction conditions
Spot is apparent, and the miscellaneous point in forward position is small.
2nd, the investigation of color developing agent
Investigation condition is as follows：
5% phosphomolybdic acid ethyl alcohol, as shown in Figure 5.
10% phosphomolybdic acid ethyl alcohol, as shown in Figure 6.
It investigates the results show that when color developing agent selects 10% phosphomolybdic acid ethyl alcohol, colour developing is efficient, with 5% phosphomolybdic acid ethyl alcohol
Color developing agent needs to dry about eight minutes to spot development removing, and is dried about 4,5 minutes or so with 10% phosphomolybdic acid ethyl alcohol color developing agent
The clear spot of color can occur, heating time is short than 5%, it is not easy to paste plate, colour developing becomes apparent from.It is therefore preferable that
10% phosphomolybdic acid ethyl alcohol is as color developing agent.
3rd, the investigation of point sample amount
The investigation condition of reference substance solution point sample amount is as follows：
Prefabricated board：1、2、3、5μL.
Make silica gel thin-layer plate by oneself：2、4、5μL.
The investigation condition of test solution point sample amount is as follows：
Prefabricated board：1、2、3、5μL.
Make silica gel thin-layer plate by oneself：2、4、5、7、10μL.
The results are shown in Figure 7 for prefabricated board, and making silica gel thin-layer plate by oneself, the results are shown in Figure 8.
It investigates the results show that as methanol eddy 1.5h, during with 5 μ L point samples, point sample amount is small, and point sample number is few, does not trail, mesh
Marking spot separation is clear.When reference substance concentration is 2mg/mL, with 2 μ L point samples, point sample amount is small, and point sample number is few, and economical and practical.
4th, the investigation of solvent
Investigation condition is as follows：
Chloroform：Ethyl alcohol=(95:5), as shown in Figure 9.
Chloroform：Methanol：Ethyl acetate=(90:10:10), as shown in Figure 10.
It investigates the results show that (90:10:10)=chloroform：Methanol：Ethyl acetate be optimal solvent, reference substance Rf
Value is compared with chloroform：Ethyl alcohol=(95:5) have and increase, separation becomes apparent from.
In conclusion the high eyebrow hymsleya amabilis TLC Identification of the present invention, has chosen suitable reference substance and expansion
The target component of high eyebrow hymsleya amabilis is effectively separated by condition, can accurately and reliably differentiate high eyebrow hymsleya amabilis, itself and long fruit are avenged
Courage and Pengxian County hymsleya amabilis distinguish, and effective guarantee is provided for clinical application.
Claims (10)
1. a kind of TLC Identification of high eyebrow hymsleya amabilis, it is characterised in that：Comprise the following steps：
(1) preparation of test solution：
Medicinal powder to be checked is taken, methanol extraction is added in, filters, test solution is prepared in concentration, methanol constant volume；
(2) preparation of reference substance solution：
Reference substance solution is prepared by reference substance of Hemsleyadin first；
(3) thin layer differentiates：
By reference substance solution and test solution, point sample, solvent expansion, colour developing are inspected, comparison on lamellae respectively；
The solvent is by following parts by volume into being grouped into：
90~95 parts by volume of chloroform, 5~10 parts by volume of ethyl acetate, 5~10 parts by volume of methanol.
2. TLC Identification according to claim 1, it is characterised in that：The solvent is by following parts by volume
Into being grouped into：
90 parts by volume of chloroform, 10 parts by volume of ethyl acetate, 10 parts by volume of methanol.
3. TLC Identification according to claim 1 or 2, it is characterised in that：In step (1), the extraction is
Refluxing extraction；Preferably, when the time of the refluxing extraction is 1.5 small.
4. according to claim 1-3 any one of them TLC Identifications, it is characterised in that：The first of the step (1)
In alcohol extracting, the envelope-bulk to weight ratio of methanol and medicinal material to be checked is 10mL:1g.
5. according to claim 1-3 any one of them TLC Identifications, it is characterised in that：The first of the step (1)
The envelope-bulk to weight ratio of alcohol constant volume and medicinal material to be checked is 2mL：1g.
6. according to claim 1-5 any one of them TLC Identifications, it is characterised in that：Pair of the step (2)
According to the methanol solution that product solution is Hemsleyadin first；Preferably, the concentration of Hemsleyadin first is 2mg/mL.
7. according to claim 1-5 any one of them TLC Identifications, it is characterised in that：It is described right in step (3)
Point sample amount according to product solution point sample is 2 μ L, and the point sample amount of test solution point sample is 5 μ L.
8. according to claim 1-5 any one of them TLC Identifications, it is characterised in that：It is described aobvious in step (3)
Color is using 10% phosphomolybdic acid ethanol solution as color developing agent, and colour developing is heated at 120 DEG C.
9. according to claim 1-5 any one of them TLC Identifications, it is characterised in that：In step (3), the ratio
To for following step A or/and B：
Step A, in test sample chromatography, on position corresponding with Hemsleyadin first reference substance chromatography, if the spot of aobvious same color
Point；
Step B, in test sample chromatography, if there are Rf values for 0.84 ± 0.03,0.61 ± 0.05,0.50 ± 0.04,0.28 ±
0.05th, 0.16 ± 0.04 blueness/pewter spot.
10. according to claim 1-9 any one of them TLC Identifications, it is characterised in that：The discriminating is to discriminate between
High eyebrow hymsleya amabilis and dolichocarpel hemsleya root or Pengxian County hymsleya amabilis.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101979027A (en) * | 2010-10-29 | 2011-02-23 | 四川逢春制药有限公司 | Method for detecting Jinze coronary disease capsules |
| CN102274401A (en) * | 2011-06-30 | 2011-12-14 | 贵州三仁堂药业有限公司 | Traditional Chinese medicine preparation for treating gastropathy and preparation method and detection method thereof |
-
2016
- 2016-11-07 CN CN201610975360.2A patent/CN108072730B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101979027A (en) * | 2010-10-29 | 2011-02-23 | 四川逢春制药有限公司 | Method for detecting Jinze coronary disease capsules |
| CN102274401A (en) * | 2011-06-30 | 2011-12-14 | 贵州三仁堂药业有限公司 | Traditional Chinese medicine preparation for treating gastropathy and preparation method and detection method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 施亚琴 等: "峨眉雪胆的化学成分研究", 《中草药》 * |
| 王贤英: "金泽冠心片的质量标准提高研究", 《重庆中草药研究》 * |
| 魏云: "雪胆的鉴别及含量测定", 《成都中医药大学学报》 * |
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Denomination of invention: A Thin Layer Chromatography Identification Method for Emei Snow Gall Effective date of registration: 20230831 Granted publication date: 20200114 Pledgee: Bank of Chengdu science and technology branch of Limited by Share Ltd. Pledgor: SICHUAN FUZHENG PHARMACEUTICAL CO.,LTD. Registration number: Y2023980054801 |
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