CN108072551B - Solid phase microextraction handle - Google Patents
Solid phase microextraction handle Download PDFInfo
- Publication number
- CN108072551B CN108072551B CN201611012825.0A CN201611012825A CN108072551B CN 108072551 B CN108072551 B CN 108072551B CN 201611012825 A CN201611012825 A CN 201611012825A CN 108072551 B CN108072551 B CN 108072551B
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- Prior art keywords
- push rod
- handle
- clamping
- extraction probe
- metal sleeve
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- 238000002470 solid-phase micro-extraction Methods 0.000 title claims abstract description 27
- 238000000605 extraction Methods 0.000 claims abstract description 101
- 239000000523 sample Substances 0.000 claims abstract description 94
- 239000002184 metal Substances 0.000 claims abstract description 59
- 229910052751 metal Inorganic materials 0.000 claims abstract description 59
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000010935 stainless steel Substances 0.000 claims description 8
- 230000000903 blocking effect Effects 0.000 claims description 5
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 description 12
- 238000000576 coating method Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 239000012491 analyte Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002076 thermal analysis method Methods 0.000 description 3
- 238000009834 vaporization Methods 0.000 description 3
- 230000008016 vaporization Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 238000004853 microextraction Methods 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Abstract
The invention relates to a solid-phase microextraction handle, which comprises a handle push rod, a handle barrel, a metal sleeve and an extraction probe taking a stainless steel wire as a supporting material, and is characterized in that: the handle push rod and the handle cylinder are both provided with positioning clamps, and the handle push rod is connected with the handle cylinder; the lower end of the handle cylinder is provided with a metal sleeve fixing support with a sealing gasket, and the metal sleeve fixing support is connected with the metal sleeve; the extraction probe sequentially penetrates through the metal sleeve and the sealing gasket to be connected with the handle push rod. The invention has low cost, convenient carrying, durability and easy replacement.
Description
Technical Field
The invention relates to the technical field of sample pretreatment, in particular to a solid-phase microextraction handle.
Background
In the analysis process of a complex sample of a matrix, pretreatment is an indispensable step, but the traditional sample pretreatment technology, such as liquid-liquid extraction, chromatography, distillation, headspace method, solid-phase extraction method and the like, has the defects of large solvent consumption, time and labor consumption, easy loss of volatile components, easy damage to the health of operators and the environment and the like. In order to overcome various disadvantages of the conventional sample pretreatment technique and to improve analysis efficiency, researchers have developed novel sample pretreatment techniques typified by droplet micro extraction and solid phase micro extraction (Solid Phase Microextraction, SPME).
The SPME technology integrates extraction, concentration, desorption and sample injection, can be directly combined with gas chromatography, gas chromatography-mass spectrometry, high performance liquid chromatography, capillary electrophoresis apparatus and other instruments, has the advantages of simplicity, convenience, rapidness, economy, safety, no need of solvents, high sensitivity, good selection, small required sample amount and the like, and has wide application prospect. So far, SPME is widely applied to the fields of environment, food, judicial chemical identification and the like, and can extract and separate various target analytes such as environmental pollutants, food additives, poisons, drugs, explosive residues and the like in a complex sample matrix by selecting proper coating materials and extraction modes, so that the analysis and detection speed is greatly improved.
At present, SPME research is mainly focused on development and optimization of an extraction coating, but an extraction handle serving as an extraction probe control and support carrier plays a very important role in the SPME process, according to preliminary investigation, a solid-phase microextraction handle produced by the Supelco company in the United states is mainly sold on the market in China, the cost is relatively high, and the handle uses a special extraction probe produced by the Supelco company, so that other types of extraction probes cannot be conveniently installed, and the wide application of the SPME technology in the actual analysis process is greatly limited.
In order to improve the utilization rate of the extraction handle, facilitate the use of various types of extraction probes by scientific research technicians, further regulate the exposed area of the extraction coating in the extraction process and the position of the extraction coating in a sample, and reduce the SPME cost, a solid-phase microextraction handle with the extraction probes which is low in cost, convenient to carry, durable and easy to replace is needed, and technical support is provided for further expanding the wide application of the SPME technology.
Disclosure of Invention
The invention aims to solve the technical problem of providing a solid-phase microextraction handle which is low in cost, convenient to carry, durable and easy to replace.
In order to solve the problems, the solid-phase microextraction handle comprises a handle push rod, a handle barrel, a metal sleeve and an extraction probe taking a stainless steel wire as a supporting material, and is characterized in that: the handle push rod and the handle cylinder are both provided with positioning clamps, and the handle push rod is connected with the handle cylinder; the lower end of the handle cylinder is provided with a metal sleeve fixing support with a sealing gasket, and the metal sleeve fixing support is connected with the metal sleeve; the extraction probe sequentially penetrates through the metal sleeve and the sealing gasket to be connected with the handle push rod.
The positioning clamp comprises an upper clamp with a rubber pad I stuck on the inner side and a lower clamp with a rubber pad II stuck on the inner side, which are connected together through a fixing pin and a nailing pin; the inner sides of the upper card and the lower card are arc-shaped; the pin consists of an upper pin clamp and a lower pin clamp; the upper clamp pin is connected with the lower clamp pin through the fixing pin.
The handle cylinder consists of an upper half part and a lower half part which are butted together through a thread I, and scale marks are arranged on the upper half part.
The handle push rod is connected with the handle cylinder through a rubber sleeve ring I.
The extraction probe sequentially passes through the metal sleeve and the sealing gasket and is connected with the handle push rod through the extraction probe fixing device.
The extraction probe fixing device comprises a body; the body is provided with a push rod rubber sleeve ring fixing groove I, and the end part of the body is provided with a clamping core I with a thread II; the clamping core I is matched with and sleeved with a clamping groove with a thread III; the extraction probe sequentially passes through the clamping groove and the clamping core I; the rubber lantern ring I is embedded in the rubber lantern ring fixing groove I of the push rod.
The extraction probe fixing device comprises a body; the body is provided with a push rod rubber sleeve ring fixing groove II, a spring blocking groove and a clamping core fixing groove, and the end part of the body is provided with a clamping core II; the clamping core II sequentially penetrates through the clamping cap and the spring to be connected with the clamping core fixing groove; the extraction probe passes through the clamping core II; the rubber lantern ring I is embedded in the rubber lantern ring fixing groove II of the push rod.
The handle push rod is provided with a push rod marking I.
The handle push rod comprises an outer push rod and an inner push rod penetrating through the outer push rod; one end of the outer push rod is provided with a positioning knob I, the inner wall of the outer push rod is provided with threads IV, the outer push rod is provided with outer push rod scales, and the other end of the outer push rod is provided with a rubber sleeve ring clamping groove; the rubber sleeve ring clamping groove is provided with a rubber sleeve ring II with a push rod marking II; one end of the inner push rod is provided with a positioning knob II, the outer wall of the inner push rod is provided with threads V, the inner push rod marking lines are respectively arranged on the inner push rod, and the other end of the inner push rod is provided with a clamping core III; the clamping core III sequentially passes through the clamping ring blocking groove and the stainless steel clamping ring; a rubber pad is arranged at the joint of the outer push rod and the inner push rod; the extraction probe penetrates through the metal sleeve, the sealing gasket and the clamping core III to be connected with the handle push rod.
Compared with the prior art, the invention has the following advantages:
1. the extraction probe fixing device is arranged on the handle push rod, so that the extraction probe can be conveniently replaced.
2. The handle push rod and the handle barrel are respectively provided with the positioning card easy to install, so that the length of the coating of the extraction probe and the position of the extraction probe in the sample can be continuously adjusted and fixed.
3. The invention can effectively fix the hollow fiber which is made of stainless steel wires, silicon oxide fiber and the like and is used as a supporting material, and has good compatibility.
4. The rubber pad is adhered to the inner side of the positioning clamp, so that friction between the positioning clamp and the handle push rod and friction between the positioning clamp and the handle barrel can be increased, and the handle can be effectively fixed by being matched with devices such as a iron stand and the like.
5. The inner side of the positioning card is round, so that a cylindrical handle push rod and a handle barrel can be well fixed.
6. The surface of the handle cylinder is provided with scales, and the length of the extraction probe extending out of the metal sleeve can be observed by combining the push rod marks.
7. The invention has low cost, convenient carrying and durability.
Drawings
The following describes the embodiments of the present invention in further detail with reference to the drawings.
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic diagram of the overall structure of the locator card according to the present invention.
Fig. 3 is a schematic view of a card-on-card structure of the positioning card of the present invention.
Fig. 4 is a schematic structural diagram of a lower card of the positioning card in the present invention.
Fig. 5 is a schematic structural view of an extraction handle barrel in the invention.
FIG. 6 is a schematic structural view of an extraction probe fixing device in the present invention.
Fig. 7 is a schematic structural diagram of an extraction probe using stainless steel wires as supporting materials in the invention.
Fig. 8 is a schematic view of another embodiment of the present invention.
FIG. 9 is a schematic view of another extraction probe securing device according to the present invention.
Fig. 10 is a schematic view of a third construction of the present invention.
FIG. 11 is a schematic view of a handle push rod according to the present invention.
In the figure: 1-a handle push rod; 101-an outer push rod; 111-positioning knob i; 112-thread iv; 113-push rod scale; 114-a rubber collar clamping groove; 102-an inner push rod; 121—a positioning knob ii; 122-rubber pad; 123—inner pushrod markings; 124-thread v; 125-stainless steel snap ring; 126-a snap ring retaining groove; 127-core III; 2-positioning card; 21-fixing pins; 22-pinning; 221-upper staple pin; 222-lower staple pin; 23-loading; 24-lower clamping; 25-a rubber pad I; 26-a rubber pad II; 3-a handle cylinder; 31-upper half; 32-lower half; 4-an extraction probe; 5-scale marks; 6-a rubber sleeve ring I; 7-push rod mark line I; 8-an extraction probe fixing device; 81-a push rod rubber sleeve ring fixing groove I; 82-thread ii; 83-a clamping core I; 84-thread III; 811-a push rod rubber sleeve ring fixing groove II; 821-spring catch groove; 831-a clamping core fixing groove; 861-clip core ii; 851-clip cap; 841-spring; 9, a metal sleeve fixing support; 10-a sealing gasket; 11-metal sleeve.
Detailed Description
Embodiment 1 as shown in fig. 1 to 7, the solid-phase microextraction handle comprises a handle push rod 1, a handle cylinder 3, a metal sleeve 11 and an extraction probe 4 taking stainless steel wires as supporting materials.
The handle push rod 1 and the handle cylinder 3 are provided with a positioning clamp 2, and the handle push rod 1 is connected with the handle cylinder 3 through a rubber sleeve ring I6; the lower end of the handle cylinder 3 is provided with a metal sleeve fixing support 9 with a sealing gasket 10, and the metal sleeve fixing support 9 is connected with a metal sleeve 11; the extraction probe 4 sequentially passes through the metal sleeve 11 and the sealing gasket 10 and is connected with the handle push rod 1 through the extraction probe fixing device 8.
Wherein:
the positioning clamp 2 comprises an upper clamp 23 with a rubber gasket I25 adhered to the inner side and a lower clamp 24 with a rubber gasket II 26 adhered to the inner side, which are connected together through a fixing pin 21 and a pin 22; the inner sides of the upper card 23 and the lower card 24 are arc-shaped; the staple pin 22 is composed of an upper staple pin 221 and a lower staple pin 222; the upper clamp pin 221 is connected to the lower clamp pin 222 by the fixing pin 21.
The handle cylinder 3 consists of an upper half 31 and a lower half 32 which are butted together by a thread i, the upper half 31 being provided with graduation marks 5.
The extraction probe fixing device 8 includes a body; the body is provided with a push rod rubber sleeve ring fixing groove I81, and the end part of the body is provided with a clamping core I83 with a thread II 82; the clamping core I83 is matched with and sleeved with a clamping groove with a thread III 84; the extraction probe 4 sequentially passes through the clamping groove and the clamping core I83; the rubber lantern ring I6 is embedded in the rubber lantern ring fixing groove I81 of the push rod.
The handle push rod 1 is provided with a push rod marking I7.
The application method of the invention is as follows:
taking out the handle push rod 1, and embedding a rubber sleeve ring I6 into a push rod rubber sleeve ring fixing groove 81; the gasket 10 is fitted into the metal sleeve mount 9.
Secondly, the extraction probe 4 penetrates through the metal sleeve 11 from the end without the coating, and is inserted into the lower half 32 of the handle cylinder 3 through the metal sleeve 11 and the sealing gasket 10; and then the extraction probe 4 is inserted into the clamping core 83, the insertion depth is adjusted, when the push rod marking I7 is positioned at the 0 scale of the scale mark 5, the front end of the coating of the extraction probe 4 is just not exposed out of the metal sleeve 11, the clamping groove is screwed, and the extraction probe 4 is fixed on the handle push rod 1.
The lower half 32 of the handle cylinder 3 is connected with the upper half 31 through the threads I, the extraction handle is fixed on a proper metal support according to actual needs, the extraction probe 4 is stretched into an actual sample through the handle push rod 1, the actual exposure length of the extraction probe 4 is observed through the push rod marking I7 and the scale mark 5, and the positioning clamp 2 is respectively arranged at proper positions of the handle push rod 1 and the handle cylinder 3 and used for fixing the same coating length and the same position of the extraction probe 4 in each extraction process. After the extraction is finished, the extraction probe 4 is completely retracted into the metal sleeve 11 through the handle push rod 1, the metal sleeve 11 is inserted into the gas chromatograph vaporization chamber, the extraction probe 4 is pushed out, after the thermal analysis of the target analyte is finished, the extraction probe 4 is retracted into the metal sleeve 11, the extraction handle is pulled out, and the sample injection is finished.
Embodiment 2 as shown in fig. 2 to 5 and fig. 7 to 9, the solid-phase microextraction handle comprises a handle push rod 1, a handle cylinder 3, a metal sleeve 11 and an extraction probe 4 taking a stainless steel wire as a supporting material.
The handle push rod 1 and the handle cylinder 3 are provided with a positioning clamp 2, and the handle push rod 1 is connected with the handle cylinder 3 through a rubber sleeve ring I6; the lower end of the handle cylinder 3 is provided with a metal sleeve fixing support 9 with a sealing gasket 10, and the metal sleeve fixing support 9 is connected with a metal sleeve 11; the extraction probe 4 sequentially passes through the metal sleeve 11 and the sealing gasket 10 and is connected with the handle push rod 1 through the extraction probe fixing device 8.
Wherein:
the positioning clamp 2 comprises an upper clamp 23 with a rubber gasket I25 adhered to the inner side and a lower clamp 24 with a rubber gasket II 26 adhered to the inner side, which are connected together through a fixing pin 21 and a pin 22; the inner sides of the upper card 23 and the lower card 24 are arc-shaped; the staple pin 22 is composed of an upper staple pin 221 and a lower staple pin 222; the upper clamp pin 221 is connected to the lower clamp pin 222 by the fixing pin 21.
The handle cylinder 3 consists of an upper half 31 and a lower half 32 which are butted together by a thread i, the upper half 31 being provided with graduation marks 5.
The extraction probe fixing device 8 includes a body; the body is provided with a push rod rubber sleeve ring fixing groove II 811, a spring blocking groove 821 and a clamping core fixing groove 831, and the end part of the body is provided with a clamping core II 861; the clamping core II 861 sequentially passes through the clamping cap 851 and the spring 841 to be connected with the clamping core fixing groove 831; the extraction probe 4 passes through the clamping core II 861; the rubber lantern ring I6 is embedded in the rubber lantern ring fixing groove II 811 of the push rod.
The handle push rod 1 is provided with a push rod marking I7.
The application method of the invention is as follows:
taking out extraction push rod 1, installing spring 841, clamping cap 851 and clamping core II 861 on handle push rod 1 in sequence, forcefully embedding clamping core II 861 into clamping core fixing groove 831, loading handle push rod 1 into upper half 31 of handle cylinder 3, sleeving rubber sleeve I6 into push rod rubber sleeve fixing groove II 811, and finally fixing handle push rod 1 into upper half 31 of handle cylinder 3 through rubber sleeve I6.
Secondly, the extraction probe 4 penetrates through the metal sleeve 11 from the end without the coating, and is inserted into the lower half 32 of the handle cylinder 3 through the metal sleeve 11 and the sealing gasket 10; after the clamping core II 861 is completely opened, the extraction probe 4 with a proper length is inserted into the clamping core II 861, the push rod marking I7 is positioned at the 0 scale of the scale mark 5, the front end of the coating of the extraction probe 4 is just not exposed out of the metal sleeve 11, and the extraction probe 4 is fixed on the handle push rod 1 by loosening the clamping cap 851.
The lower half 32 of the handle cylinder 3 is connected with the upper half 31 through the threads I, the extraction handle is fixed on a proper metal support according to actual needs, the extraction probe 4 is stretched into an actual sample through the handle push rod 1, the actual exposure length of the extraction probe 4 is observed through the push rod marking I7 and the scale mark 5, and the positioning clamp 2 is respectively arranged at proper positions of the handle push rod 1 and the handle cylinder 3 and used for fixing the same coating length and the same position of the extraction probe 4 in each extraction process. After the extraction is finished, the extraction probe 4 is completely retracted into the metal sleeve 11 through the handle push rod 1, the metal sleeve 11 is inserted into the gas chromatograph vaporization chamber, the extraction probe 4 is pushed out, after the thermal analysis of the target analyte is finished, the extraction probe 4 is retracted into the metal sleeve 11, the extraction handle is pulled out, and the sample injection is finished.
Embodiment 3 as shown in fig. 2 to 5, fig. 7 and fig. 10 to 11, the solid-phase microextraction handle comprises a handle push rod 1, a handle cylinder 3, a metal sleeve 11 and an extraction probe 4 using a stainless steel wire as a supporting material.
The handle push rod 1 and the handle cylinder 3 are provided with positioning clamps 2, and the handle push rod 1 is connected with the handle cylinder 3; the lower end of the handle cylinder 3 is provided with a metal sleeve fixing support 9 with a sealing gasket 10, and the metal sleeve fixing support 9 is connected with a metal sleeve 11; the extraction probe 4 sequentially passes through the metal sleeve 11 and the sealing gasket 10 to be connected with the handle push rod 1.
Wherein:
the positioning clamp 2 comprises an upper clamp 23 with a rubber gasket I25 adhered to the inner side and a lower clamp 24 with a rubber gasket II 26 adhered to the inner side, which are connected together through a fixing pin 21 and a pin 22; the inner sides of the upper card 23 and the lower card 24 are arc-shaped; the staple pin 22 is composed of an upper staple pin 221 and a lower staple pin 222; the upper clamp pin 221 is connected to the lower clamp pin 222 by the fixing pin 21.
The handle cylinder 3 consists of an upper half 31 and a lower half 32 which are butted together by a thread i, the upper half 31 being provided with graduation marks 5.
The handle push rod 1 comprises an outer push rod 101 and an inner push rod 102 penetrating through the outer push rod 101; one end of the outer push rod 101 is provided with a positioning knob I111, the inner wall of the outer push rod is provided with a thread IV 112, an outer push rod scale 113 is arranged on the outer push rod, and the other end of the outer push rod is provided with a rubber sleeve ring clamping groove 114; a rubber sleeve II with a push rod mark II is arranged on the rubber sleeve clamping groove 114; one end of the inner push rod 102 is provided with a positioning knob II 121, the outer wall of the inner push rod is provided with a thread V124, the inner push rod marking lines 123 are respectively arranged on the inner push rod, and the other end of the inner push rod is provided with a clamping core III 127; the clamping core III 127 sequentially passes through the clamping ring retaining groove 126 and the stainless steel clamping ring 125; a rubber pad 122 is arranged at the joint of the outer push rod 101 and the inner push rod 102; the extraction probe 4 passes through the metal sleeve 11, the sealing gasket 10 and the clamping core III 127 to be connected with the handle push rod 1.
The application method of the invention is as follows:
unscrewing the positioning knob I111 and removing the rubber pad 122 in the knob; unscrewing the handle cylinder 3, taking out the handle push rod 1, and unscrewing the inner push rod 102 from the outer push rod 101; the extraction probe 4 is inserted into the lower half 32 of the handle cylinder 3 from the uncoated end through the metal sleeve 11 via the gasket 10; the extraction probe 4 is inserted into the clamping core III 127, the insertion depth is adjusted, the push rod marking II is just positioned at the 0 position of the push rod scale when the metal sleeve 11 is not exposed at the coating end of the extraction probe 4 in the state that the extraction handle is installed, and the stainless steel clamping ring 125 is pushed to the middle of the clamping ring blocking groove 126 and the clamping core III 127, so that the extraction probe 4 is fixed on the handle push rod 1.
The handle push rod 1 with the extraction probe 4 fixed is installed in the upper half part 31 of the handle barrel 3, the upper part and the lower part of the handle are screwed, the rubber pad 122 is embedded into the positioning knob I111, and then the rubber pad is screwed on the handle push rod 1.
According to actual needs, the extension length of the extraction probe 4 is determined by using the inner push rod marking 123 and the outer push rod marking 113, and the position when the extraction probe 4 is completely retracted into the metal sleeve 11 is determined by using the push rod marking II and the handle cylinder 3 marking 5; the extraction handle is fixed on a proper metal support, the extraction probe 4 is stretched into an actual sample through the handle push rod 1, the position of the extraction handle cylinder 3 is marked by the positioning card 2, after extraction is finished, the push rod mark II is drawn to a previously calibrated position, so that the extraction probe 4 is completely retracted into the metal sleeve 11, the metal sleeve 11 is inserted into the gas chromatograph vaporization chamber, the extraction probe 4 is pushed out, after thermal analysis of a target analyte is finished, the push rod mark II is drawn to the previously calibrated position again, the extraction handle is pulled out, and sample injection is finished.
Claims (6)
1. The solid-phase microextraction handle comprises a handle push rod (1), a handle barrel (3), a metal sleeve (11) and an extraction probe (4) taking a stainless steel wire as a supporting material, and is characterized in that: the handle push rod (1) and the handle cylinder (3) are provided with positioning clamps (2), and the handle push rod (1) is connected with the handle cylinder (3); the lower end of the handle cylinder (3) is provided with a metal sleeve fixing support (9) with a sealing gasket (10), and the metal sleeve fixing support (9) is connected with the metal sleeve (11); the extraction probe (4) sequentially penetrates through the metal sleeve (11) and the sealing gasket (10) to be connected with the handle push rod (1); the handle cylinder (3) consists of an upper half part (31) and a lower half part (32) which are butted together through a thread I, and graduation marks (5) are arranged on the upper half part (31); a push rod marking I (7) is arranged on the handle push rod (1); the handle push rod (1) comprises an outer push rod (101) and an inner push rod (102) penetrating through the outer push rod (101); one end of the push rod (101) is provided with a positioning knob I (111), the inner wall of the push rod is provided with a thread IV (112), the push rod is provided with push rod scales (113), and the other end of the push rod is provided with a rubber sleeve ring clamping groove (114); a rubber sleeve II with a push rod mark II is arranged on the rubber sleeve clamping groove (114); one end of the inner push rod (102) is provided with a positioning knob II (121), the outer wall of the inner push rod is provided with a thread V (124), the inner push rod marking lines (123) are respectively arranged on the inner push rod, and the other end of the inner push rod is provided with a clamping core III (127); the clamping core III (127) sequentially passes through the clamping ring retaining groove (126) and the stainless steel clamping ring (125); a rubber pad (122) is arranged at the joint of the outer push rod (101) and the inner push rod (102); the extraction probe (4) passes through the metal sleeve (11), the sealing gasket (10) and the clamping core III (127) to be connected with the handle push rod (1).
2. The solid phase microextraction handle of claim 1, wherein: the positioning card (2) comprises an upper card (23) with a rubber pad I (25) adhered to the inner side and a lower card (24) with a rubber pad II (26) adhered to the inner side, which are connected together through a fixing pin (21) and a pin (22); the inner sides of the upper card (23) and the lower card (24) are arc-shaped; the pin (22) consists of an upper pin (221) and a lower pin (222); the upper clamp pin (221) is connected with the lower clamp pin (222) through the fixing pin (21).
3. The solid phase microextraction handle of claim 1, wherein: the handle push rod (1) is connected with the handle cylinder (3) through a rubber sleeve ring I (6).
4. The solid phase microextraction handle of claim 1, wherein: the extraction probe (4) sequentially penetrates through the metal sleeve (11) and the sealing gasket (10) and is connected with the handle push rod (1) through the extraction probe fixing device (8).
5. The solid phase microextraction handle according to claim 4, wherein: the extraction probe fixing device (8) comprises a body; the body is provided with a push rod rubber sleeve ring fixing groove I (81), and the end part of the body is provided with a clamping core I (83) with a thread II (82); the clamping core I (83) is matched with a clamping groove sleeved with a thread III (84); the extraction probe (4) sequentially passes through the clamping groove and the clamping core I (83); the rubber sleeve ring I (6) is embedded in the rubber sleeve ring fixing groove I (81) of the push rod.
6. The solid phase microextraction handle according to claim 4, wherein: the extraction probe fixing device (8) comprises a body; the body is provided with a push rod rubber sleeve ring fixing groove II (811), a spring blocking groove (821) and a clamping core fixing groove (831), and the end part of the body is provided with a clamping core II (861); the clamping core II (861) sequentially penetrates through the clamping cap (851) and the spring (841) to be connected with the clamping core fixing groove (831); the extraction probe (4) passes through the clamping core II (861); the rubber sleeve I (6) is embedded in the rubber sleeve fixing groove II (811) of the push rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611012825.0A CN108072551B (en) | 2016-11-17 | 2016-11-17 | Solid phase microextraction handle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611012825.0A CN108072551B (en) | 2016-11-17 | 2016-11-17 | Solid phase microextraction handle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108072551A CN108072551A (en) | 2018-05-25 |
| CN108072551B true CN108072551B (en) | 2024-03-26 |
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