CN108070462B

CN108070462B - Biodegradable environment-friendly glass cleaning fluid

Info

Publication number: CN108070462B
Application number: CN201810039671.7A
Authority: CN
Inventors: 史莹飞; 文昌; 卡尔·汉斯; 乔伊·马克斯
Original assignee: Jiangsu Lopal Tech Co ltd
Current assignee: Jiangsu Longpan New Material Technology Co ltd
Priority date: 2018-01-16
Filing date: 2018-01-16
Publication date: 2020-10-09
Anticipated expiration: 2038-01-16
Also published as: CN108070462A

Abstract

The invention discloses a biodegradable environment-friendly glass cleaning solution which comprises the following components in parts by weight: 3-8 parts of biological surfactant, 0.1-0.5 part of biological enzyme compound agent, 1-5 parts of biological corrosion inhibitor, 70-90 parts of deionized water and 5-20 parts of freezing point depressant. The glass cleaning liquid provided by the invention has the advantages that the biological surfactant is used for replacing a chemical surfactant in the traditional glass water, the biological enzyme compound agent is used for removing shellac, and the biological enzyme corrosion inhibitor is used for realizing corrosion inhibition, so that the glass cleaning liquid can not only realize decontamination, anti-freezing, shellac removal, corrosion prevention and the like, but also realize complete biodegradation, has zero pollution to the environment, is almost harmless to human beings, animals, plants and the like, is safe and environment-friendly.

Description

Biodegradable environment-friendly glass cleaning fluid

Technical Field

The invention relates to a glass cleaning fluid, in particular to a biodegradable environment-friendly glass cleaning fluid, belonging to the field of automobile glass cleaning agents.

Background

The glass cleaning agent is commonly called glass water, and has become the most consumable product in the automobile industry along with the vigorous development of the automobile industry in recent years. The glass water is mainly used for cleaning the front and rear windshields of the automobile and is generally matched with a wiper for use. A plurality of high-molecular organic matters which are difficult to degrade are mostly used in the traditional glass water formula, and the high-molecular organic matters flow into soil along with the use process of the glass water and enter underground water, although the glass water consumption of a single automobile is not large, the glass water can be accumulated to a large extent through the enrichment effect, and finally the soil and the underground water are greatly polluted, so that the environmental protection concept of sustainable development is violated.

Chinese patent ZL2011101976031 discloses a biodegradable glass cleaning liquid product, which comprises the following components: 0.5 to 15 wt% of a microorganism of the genus Bacillus, 1 to 30 wt% of a microorganism of the genus Pseudomonas, 1 to 20 wt% of a surfactant, and 0.5 to 15 wt% of an inorganic salt, wherein the microorganism of the genus Bacillus and the microorganism of the genus Pseudomonas are in the form of spores. In the patent, various microorganisms such as bacillus microorganisms can survive in the conventional glass cleaning solution, and can secrete various extracellular enzymes during the growth period, and the enzymes can degrade organic matters in the glass cleaning solution into nutrient substances required by the growth of the glass cleaning solution, so that the environment is purified.

However, the glass cleaning solution in the patent still uses a chemical surfactant, such as an anionic surfactant, and the cleaned liquid still pollutes soil and basement, so that the problem of environmental pollution cannot be fundamentally solved.

Disclosure of Invention

The purpose of the invention is as follows: the invention aims to provide the environment-friendly glass cleaning solution which can thoroughly realize biodegradation.

The technical scheme is as follows: the biodegradable environment-friendly glass cleaning solution comprises the following components in parts by weight: 3-8 parts of biological surfactant, 0.1-0.5 part of biological enzyme compound agent, 1-5 parts of biological corrosion inhibitor, 50-80 parts of water and 10-40 parts of freezing point depressant. The water mainly refers to a water source without metal ions, the windshield is prevented from being scraped after precipitation, and the deionized water is the lowest in cost and is universal in the industry.

The biosurfactant comprises one of glycolipid, lipopeptide and fatty acid biosurfactant, preferably the lipopeptide biosurfactant, can be prepared by biological fermentation and purification of Bacillus subtilis LPB-3, wherein the classification name of the Bacillus subtilis LPB-3 is Bacillus subtilis, the strain is preserved in China general microbiological culture Collection center (CGMCC) in 12 and 4 months in 2017, and the preservation number is CGMCC No. 14375; of course, lipopeptide biosurfactants are also commercially available.

Lipopeptides are compounds consisting of hydrophilic peptide chains and lipophilic aliphatic hydrocarbon chains, have excellent surfactant performance, are prepared by purifying microbial fermentation products, and are completely biodegradable.

The biological enzyme compound agent contains at least one of protease, lipase, amylase, cellulase, pectinase, catalase and lysozyme. Specifically, the biological enzyme compound agent can be composed of protease, lipase, cellulase, pectinase and lysozyme, and the weight ratio of the protease, the lipase, the cellulase, the pectinase and the lysozyme is 1-10: 1-8: 1-3: 1-5: 1. In the invention, the biological enzyme compound agent can be called as deinsectization glue, the shellac contains pollutants such as excrements of insect bodies and birds, and the main components are protein, fat, resin, pigment and the like, and can be removed by the biological enzyme compound agent.

The biological corrosion inhibitor comprises at least one of an amino acid corrosion inhibitor, a rosin derivative corrosion inhibitor and a marine organism extract corrosion inhibitor, and specifically comprises the amino acid corrosion inhibitor consisting of lysine, arginine and aspartic acid, wherein the weight ratio of the lysine to the arginine to the aspartic acid is 1-5: 1. The principle of the biological corrosion inhibitor is that a large number of active groups on a biological macromolecular substance are adsorbed on the surface of a metal and then form a film to protect the surface of the metal substance and realize the corrosion inhibition effect.

The freezing point depressant contains at least one of methanol, ethanol and glycol, wherein the methanol, the ethanol and the glycol are dissolved in water in any proportion, so that the freezing point of the glass water can be remarkably reduced, the anti-freezing performance and the defrosting performance of the glass water are improved, the cost is low, and the biodegradation is easy. Preferably, the freezing point depressant can be composed of methanol and ethanol, and the weight ratio of the methanol to the ethanol is 1-5: 1.

Has the advantages that: compared with the prior art, the glass cleaning liquid provided by the invention has the advantages that the biological surfactant is used for replacing the chemical surfactant in the traditional glass water, the biological enzyme compound agent is used for removing shellac, and the biological enzyme corrosion inhibitor is used for realizing corrosion inhibition, so that the glass cleaning liquid can not only realize decontamination, freeze prevention, shellac removal, corrosion prevention and the like, but also realize complete biodegradation, has zero pollution to the environment, is almost harmless to human beings, animals and plants and the like, is safe and environment-friendly.

Detailed Description

The technical solution of the present invention is further illustrated by the following examples.

The raw materials of the glass cleaning fluid can be self-made lipopeptide biosurfactant or can be obtained on the market; other raw materials are obtained from the market and are calculated according to the parts by weight.

The self-made method of the lipopeptide biosurfactant comprises the following steps: the inventor separates withered wood leaves decayed in national forest parks of Nanjing old mountains, performs compound mutagenesis by ultraviolet rays, microwaves, diethyl sulfate and protoplasts, and then screens the leaves to obtain the Bacillus subtilis LPB-3, which is classified and named as Bacillus subtilis and is preserved in China general microbiological culture Collection center (CGMCC) in 2017, 12 and 4 days, and the preservation number is CGMCC No. 14375. Fermenting and culturing the bacillus subtilis LPB-3, separating and purifying to obtain the lipopeptide biosurfactant, wherein the purity of the biosurfactant is as high as 80-95%.

The method for preparing the lipopeptide biosurfactant specifically comprises the following steps:

(1) inoculating the bacillus subtilis LPB-3 strain into an LB solid culture medium for activation, wherein the culture temperature is 32-37 ℃, and standing for 16-24 h;

(2) performing primary seed amplification culture on the activated slant strains at the culture temperature of 32-37 ℃ and the rotation speed of 150-;

(3) performing secondary seed amplification culture on the primary seed liquid according to the inoculation amount of 1-5%, wherein the culture temperature is 32-37 ℃, the rotation speed is 100-;

(4) fermenting and culturing the secondary seed liquid according to the inoculum size of 5-10%, wherein the culture temperature is 32-37 ℃, the initial pH is 6.5-7.0, the rotation speed is 50-100rpm, the ventilation volume is 0.5-1vvm, the pressure is 0.02-0.04MPa, and the culture period is 36-48h, so as to obtain a fermentation liquid;

(5) and (3) centrifuging the fermentation liquor at low temperature, filtering and adsorbing by resin to prepare the lipopeptide biosurfactant.

In the step (1), the culture medium contains a carbon source and a nitrogen source, wherein the carbon source comprises at least one of glucose, corn steep liquor powder, molasses, glycerol and glucose syrup; the nitrogen source comprises at least one of ammonium sulfate, soybean powder, yeast extract, peptone, sodium nitrate, sodium glutamate, and ammonia water. The culture medium also contains trace elements including glycine and glutamic acidTryptophan, lysine and vitamin B₁Vitamin B₆Vitamin B₁₂At least one of (1). The culture medium also comprises inorganic salt, wherein the inorganic salt comprises at least one of magnesium sulfate, potassium chloride, sodium chloride, calcium chloride, potassium dihydrogen phosphate and dipotassium hydrogen phosphate.

In the step (5), the fermentation liquor is centrifuged for 10-20min at 1-5 ℃ and 4000-10000rpm, the supernatant is collected, a PES ultrafiltration membrane of 50-200kDa is used for multiple times of ultrafiltration, and finally macroporous adsorption resin is used for adsorption and elution to obtain the lipopeptide biosurfactant; wherein, the macroporous absorption resin can be D101 macroporous absorption resin, AB-8 macroporous absorption resin or X-5 macroporous absorption resin, and is preferably X-5 macroporous absorption resin.

Examples 1-5 are fermentation and isolation in a 100L fermentor to produce lipopeptide biosurfactants.

Example 1

Inoculating a Bacillus subtilis strain LPB-3 in LB test tube slant culture medium for activation, culturing at 32 deg.C, and standing for 24 hr. The activated slant strain is inoculated into a 2L shake flask for primary seed amplification culture, the culture temperature is 32 ℃, the rotation speed of a shaking table is 150rpm, and the culture period is 10 h. Inoculating the primary seed solution into a 10L seeding tank according to the inoculation amount of 1% for secondary seed amplification culture, wherein the culture temperature is 32 ℃, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.02MPa, and the culture period is 8 h. Inoculating the secondary seed liquid into a 100L fermentation tank according to the inoculation amount of 5% for fermentation culture, wherein the culture temperature is 32 ℃, the initial pH is 6.5, the rotation speed is 50rpm, the ventilation volume is 0.5vvm, the tank pressure is 0.02MPa, and the culture period is 36 h. The resulting fermentation broth was centrifuged at 4000rpm at 1 ℃ for 10min, and the supernatant was collected. And then PES (polyether sulfone) ultrafiltration membrane with the molecular weight cutoff of 50kDa is used for ultrafiltration, and lipopeptide macromolecular micelles are cut off from the centrifugal supernatant. And finally, adsorbing by using D101 macroporous adsorption resin and eluting to obtain lipopeptide biosurfactant stock solution. And (3) determining the yield and purity of the lipopeptide biosurfactant in the stock solution.

Example 2

Inoculating a Bacillus subtilis strain LPB-3 in LB test tube slant culture medium for activation, culturing at 35 deg.C, and standing for 24 hr. The activated slant strain is inoculated into a 2L shake flask for primary seed amplification culture, the culture temperature is 35 ℃, the rotation speed of a shaking table is 200rpm, and the culture period is 15 h. And (3) inoculating the primary seed solution into a 10L seeding tank according to the inoculation amount of 4% for secondary seed amplification culture, wherein the culture temperature is 35 ℃, the rotation speed is 150rpm, the ventilation volume is 1.5vvm, the tank pressure is 0.04MPa, and the culture period is 12 h. Inoculating the secondary seed liquid into a 100L fermentation tank according to the inoculation amount of 8% for fermentation culture, wherein the culture temperature is 35 ℃, the initial pH is 7.0, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the culture period is 48 h. Centrifuging the obtained fermentation liquid at 5 deg.C and 10000rpm for 20min, and collecting supernatant. And then PES (polyether sulfone) ultrafiltration membrane with the molecular weight cutoff of 200kDa is used for ultrafiltration, and lipopeptide macromolecular micelles are cut off from the centrifugal supernatant. And finally, adsorbing by using D101 macroporous adsorption resin and eluting to obtain lipopeptide biosurfactant stock solution. And (3) determining the yield and purity of the lipopeptide biosurfactant in the stock solution.

Example 3

Inoculating a Bacillus subtilis strain LPB-3 in LB test tube slant culture medium for activation, culturing at 35 deg.C, and standing for 24 hr. The activated slant strain is inoculated into a 2L shake flask for primary seed amplification culture, the culture temperature is 35 ℃, the rotation speed of a shaking table is 150rpm, and the culture period is 12 h. Inoculating the primary seed liquid into a 10L seeding tank according to the inoculation amount of 5% for secondary seed amplification culture, wherein the culture temperature is 35 ℃, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the culture period is 8 h. Inoculating the secondary seed liquid into a 100L fermentation tank according to the inoculation amount of 10% for fermentation culture, wherein the culture temperature is 35 ℃, the initial pH is 6.5, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the culture period is 48 h. The obtained fermentation broth was centrifuged at 8000rpm at 2 ℃ for 15min, and the supernatant was collected. And then a PES (polyether sulfone) ultrafiltration membrane with the molecular weight cutoff of 100kDa is used for ultrafiltration, lipopeptide macromolecular micelles and macromolecular heteroproteins are cut off from centrifugal supernatant, micelle dispersing agent methanol is added into the supernatant to disperse the lipopeptide micelles into monomers, and then the membrane is adopted for separation to cut off and separate the macromolecular proteins. And finally, adsorbing by using AB-8 macroporous adsorption resin and then eluting to obtain lipopeptide biosurfactant stock solution. And (3) determining the yield and purity of the lipopeptide biosurfactant in the stock solution.

Example 4

Inoculating a Bacillus subtilis strain LPB-3 in LB test tube slant culture medium for activation, and standing at 37 deg.C for 24 hr. The activated slant strain is inoculated into a 2L shake flask for primary seed amplification culture, the culture temperature is 37 ℃, the rotation speed of a shaking table is 200rpm, and the culture period is 15 h. Inoculating the primary seed liquid into a 10L seeding tank according to the inoculation amount of 5% for secondary seed amplification culture, wherein the culture temperature is 37 ℃, the rotation speed is 150rpm, the ventilation volume is 1.5vvm, the tank pressure is 0.04MPa, and the culture period is 12 h. Inoculating the secondary seed liquid into a 100L fermentation tank according to the inoculation amount of 10% for fermentation culture, wherein the culture temperature is 37 ℃, the initial pH is 7.0, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the culture period is 48 h. The resulting fermentation broth was centrifuged at 8000rpm at 3 ℃ for 10min, and the supernatant was collected. And then, performing ultrafiltration by using a PES (polyether sulfone) ultrafiltration membrane with the molecular weight cutoff of 150kDa, cutting off lipopeptide macromolecular micelles and macromolecular heteroproteins from centrifugal supernatant, adding a micelle dispersing agent methanol into the supernatant to disperse the lipopeptide micelles into monomers, and separating by using the membrane to cut off and separate the macromolecular proteins. And finally, adsorbing by using X-5 macroporous adsorption resin and eluting to obtain lipopeptide biosurfactant stock solution. And (3) determining the yield and purity of the lipopeptide biosurfactant in the stock solution.

Example 5

Inoculating a Bacillus subtilis strain LPB-3 in LB test tube slant culture medium for activation, culturing at 35 deg.C, and standing for 24 hr. The activated slant strain is inoculated into a 2L shake flask for primary seed amplification culture, the culture temperature is 35 ℃, the rotation speed of a shaking table is 150rpm, and the culture period is 12 h. And (3) inoculating the primary seed solution into a 10L seeding tank according to the inoculation amount of 4% for secondary seed amplification culture, wherein the culture temperature is 35 ℃, the rotation speed is 120rpm, the ventilation volume is 1.2vvm, the tank pressure is 0.04MPa, and the culture period is 8 h. Inoculating the secondary seed liquid into a 100L fermentation tank according to the inoculation amount of 10% for fermentation culture, wherein the culture temperature is 35 ℃, the initial pH is 6.5, the rotation speed is 75rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the culture period is 48 h. The resulting fermentation broth was centrifuged at 8000rpm for 20min at 4 ℃ to collect the supernatant. And then, performing ultrafiltration by using a PES (polyether sulfone) ultrafiltration membrane with the molecular weight cutoff of 150kDa, cutting off lipopeptide macromolecular micelles and macromolecular heteroproteins from centrifugal supernatant, adding a micelle dispersing agent methanol into the supernatant to disperse the lipopeptide micelles into monomers, and separating by using the membrane to cut off and separate the macromolecular proteins. And finally, adsorbing by using X-5 macroporous adsorption resin and eluting to obtain lipopeptide biosurfactant stock solution. And (3) determining the yield and purity of the lipopeptide biosurfactant in the stock solution.

The products of the above examples and comparative examples were tested by the following experimental methods, in which the measurement method or calculation method of the physical quantity or index concerned was carried out according to the methods described below if not specifically mentioned.

Method for measuring the yield of lipopeptide biosurfactant (HPLC method): the sample is filtered by a 0.2 mu m water system microporous filter membrane, the sample amount is 20 mu L, the ultraviolet detection wavelength is 205nm, the chromatographic column is a 4.6mm multiplied by 25cm ODS-2 reversed phase hydrophobic column, the mobile phase is acetonitrile: acetic acid (10g/L) ═ 4:1, column temperature 30 ℃, flow rate 1.5 mL/min.

The purity of the lipopeptide biosurfactant is as follows: the mass percentage of the lipopeptide surfactant in the stock solution of the separated and purified lipopeptide surfactant.

1. Lipopeptide biosurfactant yield and purity

The lipopeptide biosurfactant production and purity of the stock solutions of the lipopeptide biosurfactants of examples 1-5 were determined, respectively, and the experimental results are shown in table 1.

TABLE 1 results of lipopeptide biosurfactant production and purity experiments

Serial number	Example 1	Example 2	Example 3	Example 4	Example 5
						yield/(g/L)	0.21	1.47	1.68	0.89	2.56
Purity (%)	80	86	90	83	95

As can be seen from the above experimental results, the lipopeptide biosurfactant stock solution prepared in example 5 has the highest yield and purity, and is the best preparation process. When different parameters are changed in the preparation process, the yield and the purity of the final lipopeptide biosurfactant are influenced.

The lipopeptide biosurfactant obtained in example 5 was formulated into glass water according to the following five formulations.

Example 5-1

Raw materials: 6 parts of lipopeptide biosurfactant, 0.5 part of a biological enzyme compound agent, 3.5 parts of a biological corrosion inhibitor, 80 parts of deionized water and 10 parts of a freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 1: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 1:1:1:1: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 1:1: 1.

Examples 5 and 2

Raw materials: 8 parts of lipopeptide biosurfactant, 0.5 part of biological enzyme compound agent, 1.5 parts of biological corrosion inhibitor, 50 parts of deionized water and 40 parts of freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 5: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 10:8:3:5: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 5:5: 1.

Examples 5 to 3

Raw materials: 5 parts of lipopeptide biosurfactant, 0.3 part of biological enzyme compound agent, 1 part of biological corrosion inhibitor, 73.7 parts of deionized water and 20 parts of freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 2: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 5:4:2:4: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 3:3: 1.

Examples 5 to 4

Raw materials: 3 parts of lipopeptide biosurfactant, 0.1 part of a biological enzyme compound agent, 5 parts of a biological corrosion inhibitor, 66.9 parts of deionized water and 25 parts of a freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 3: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 6:1:2:4: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 3:2: 1.

Examples 5 to 5

Raw materials: 5 parts of lipopeptide biosurfactant, 0.3 part of biological enzyme compound agent, 3 parts of biological corrosion inhibitor, 61.7 parts of deionized water and 30 parts of freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 3: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 4:2:1:2: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 2:2: 1.

Example 6

Raw materials: 5 parts of sophorolipid biosurfactant, 0.3 part of biological enzyme compound agent, 3 parts of biological corrosion inhibitor, 61.7 parts of deionized water and 30 parts of freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 3: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 4:2:1:2: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 2:2: 1.

Example 7

Raw materials: 5 parts of rhamnolipid biosurfactant, 0.3 part of biological enzyme compound agent, 3 parts of biological corrosion inhibitor, 61.7 parts of deionized water and 30 parts of freezing point depressant. Wherein the freezing point depressant is methanol and ethanol, and the weight ratio is 3: 1; the biological enzyme compound agent consists of protease, lipase, cellulase, pectinase and lysozyme according to the weight ratio of 4:2:1:2: 1; the biological corrosion inhibitor is an amino acid corrosion inhibitor, and the weight ratio of lysine to arginine to aspartic acid is 2:2: 1.

The preparation method comprises the following steps: the glass cleaning fluid is obtained by uniformly mixing the raw materials in parts by weight.

Comparative example 1

The commercially available automobile glass water comprises the following components in parts by weight: 47 parts of methanol, 32 parts of deionized water, 6 parts of calcium chloride dihydrate, 5 parts of sodium lauryl alcohol ether sulfate, 6 parts of sorbitol monopalmitate, 2 parts of sodium citrate and 4 parts of sodium metasilicate.

Comparative example 2

The commercially available automobile glass water comprises the following components in parts by weight: 30 parts of methanol, 8 parts of calcium chloride dihydrate, 6 parts of alkylphenol polyoxyethylene, 7 parts of sorbitol monopalmitate, 6 parts of water-soluble silicone oil and 60 parts of deionized water.

The products of the above examples and comparative examples were tested by the following experimental methods:

2. freezing point test

The experimental method and the steps are detailed in GB/T23436-2009 automobile windshield cleaning solution, and the experimental results are shown in Table 1.

TABLE 2 freezing point test results

As can be seen from the above experimental results, the biodegradable environment-friendly automobile glass water prepared in the embodiment of the invention has excellent anti-freezing performance as the glass water prepared in the comparative examples 1 and 2, the better anti-freezing performance is along with the increase of the content of the freezing point depressant, glass water with different freezing points can be prepared according to the requirements of seasons and different regions, and the tested freezing points of the embodiments 5-5, 6 and 7 can meet the requirements of most regions for use in winter. Researches show that other components in the formula have no influence on the water freezing point of the glass, the more the freezing point agent is added, the lower the freezing point is, and the excellent anti-freezing performance has no specific range, and the product is generally selected according to the temperature in winter in regions, for example, the freezing point can be from-5 ℃ in the south, the freezing point can be from-20 ℃ in the middle, and the freezing point can be from-40 ℃ in the north.

3. Metal corrosion testing

The experimental method and the steps are detailed in GB/T23436-2009 automobile windshield cleaning solution, and the experimental results are shown in Table 2.

TABLE 3 metallic Corrosion test results

As can be seen from the experimental results in the table above, compared with the glass water in comparative examples 1-2, the biodegradable environment-friendly automobile glass water prepared in the embodiment of the invention can better meet the technical requirements of GB/T23436-2009 automobile windshield glass cleaning fluid on metal corrosivity, namely, the mass variation range of the metal test piece is that the aluminum sheet is +/-0.30 mg/cm³Brass sheet +/-0.15mg/cm³And + -0.80 mg/cm of galvanized steel sheet³. Wherein the larger the mass change, the more serious the corrosion, and examples 5-1 to 5-5,

The results of the tests of examples 6-7 are much better than those of comparative examples 1-2, especially the best corrosion resistance of examples 5-5. Meanwhile, compared with the glass water in the proportion, the biodegradable environment-friendly automobile glass water disclosed by the invention has better corrosion resistance and corrosion inhibition.

4. Biodegradability test

The experimental method and the steps are detailed in GB/T19277, and the experimental results are shown in Table 3.

TABLE 4 biodegradability test Experimental results

As can be seen from the above experimental results, the biodegradable environment-friendly automobile glass water prepared in the embodiments 5-1 to 5-5 and 6-7 of the invention has excellent biodegradability compared with the glass water prepared in the comparative examples 1 and 2.

Therefore, the biodegradable environment-friendly automobile glass water can be completely biodegraded and has zero pollution to the environment.

Claims

1. The biodegradable environment-friendly glass cleaning solution is characterized by comprising the following components in parts by weight: 3-8 parts of biological surfactant, 0.1-0.5 part of biological enzyme compound agent, 1-5 parts of biological corrosion inhibitor, 50-80 parts of water and 10-40 parts of freezing point depressant; the biological surfactant is a lipopeptide biological surfactant and is prepared by biological fermentation and purification of Bacillus subtilis LPB-3, wherein the Bacillus subtilis LPB-3 is classified and named as Bacillus subtilis, the strain is preserved in China general microbiological culture Collection center (CGMCC) in 12 months and 4 days in 2017, and the preservation number is CGMCC No. 14375; the biological enzyme compound agent comprises at least one of protease, lipase, amylase, cellulase, pectinase, catalase and lysozyme; the biological corrosion inhibitor comprises at least one of an amino acid corrosion inhibitor, a rosin derivative corrosion inhibitor and a marine organism extract corrosion inhibitor; the freezing point depressant comprises at least one of methanol, ethanol, and ethylene glycol.

2. The glass cleaning liquid according to claim 1, characterized in that: the biological enzyme compound agent is composed of protease, lipase, cellulase, pectinase and lysozyme in a weight ratio of 1-10: 1-8: 1-3: 1-5: 1.

3. The glass cleaning liquid according to claim 1, characterized in that: the biological corrosion inhibitor is an amino acid corrosion inhibitor.

4. The glass cleaning liquid according to claim 3, characterized in that: the amino acid corrosion inhibitor is composed of lysine, arginine and aspartic acid in a weight ratio of 1-5: 1.

5. The glass cleaning liquid according to claim 1, characterized in that: the freezing point depressant consists of methanol and ethanol in a weight ratio of 1-5: 1.