CN108061811B - Biosensor convenient for cultivating liquid replacement - Google Patents
Biosensor convenient for cultivating liquid replacement Download PDFInfo
- Publication number
- CN108061811B CN108061811B CN201711350385.4A CN201711350385A CN108061811B CN 108061811 B CN108061811 B CN 108061811B CN 201711350385 A CN201711350385 A CN 201711350385A CN 108061811 B CN108061811 B CN 108061811B
- Authority
- CN
- China
- Prior art keywords
- pipeline
- sealing
- biosensor
- sensor body
- cushion block
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Fluid-Driven Valves (AREA)
Abstract
The invention discloses a biosensor convenient for replacement of cultivation liquid, which comprises a sensor body and a liquid storage chamber arranged on the sensor body, wherein the liquid storage chamber is a cavity sealed in the sensor body; the liquid storage device further comprises a first pipeline and a second pipeline which are arranged on the sensor body, wherein one end of the first pipeline is intersected with the top end of the cavity wall of the liquid storage chamber, and the other end of the first pipeline is intersected with the surface of the sensor body; one end of the second pipeline is intersected with the bottom end of the cavity wall of the liquid storage chamber, and the other end of the second pipeline is intersected with the surface of the sensor body; the sensor also comprises two stop valves connected to the sensor body, wherein one stop valve is used for controlling the on-off state of the first pipeline, and the other stop valve is used for controlling the on-off state of the second pipeline; the other end of the first pipeline is also connected with an air source pipe quick connector. The sensor has the characteristic of convenience in liquid replacement in the liquid storage chamber.
Description
Technical Field
The invention relates to the technical field of detection tools, in particular to a biosensor convenient for replacement of a cultivation liquid.
Background
A biosensor (biosensor) is an instrument that is sensitive to biological substances and converts their concentration into an electrical signal for detection. Is an analysis tool or system which is composed of immobilized biological sensitive material as recognition element (including enzyme, antibody, antigen, microorganism, cell, tissue, nucleic acid, etc.), proper physical and chemical transducer (such as oxygen electrode, photosensitive tube, field effect tube, piezoelectric crystal, etc.), and signal amplifying device.
Biosensors are widely used for environmental quality analysis, but most existing biosensors are laboratory products, and manual liquid adding, liquid draining and cleaning are needed. The operation process is complicated, the requirement on personnel is high, the on-line monitoring of the water quality analysis equipment is not facilitated, and the large-scale industrialization difficulty is high.
Disclosure of Invention
Aiming at the problems that the existing biosensor needs manual liquid adding, liquid discharging and cleaning, is complex in operation and has high requirements on personnel, the invention provides the biosensor convenient for replacing the cultivation liquid, and the biosensor has the characteristic of convenience in replacing the liquid in a liquid storage chamber.
The biosensor convenient for replacing the cultivation liquid solves the problems through the following technical points: the biosensor convenient for cultivating liquid replacement comprises a sensor body and a liquid storage chamber arranged on the sensor body, wherein the liquid storage chamber is a cavity sealed in the sensor body;
the liquid storage device further comprises a first pipeline and a second pipeline which are arranged on the sensor body, wherein one end of the first pipeline is intersected with the top end of the cavity wall of the liquid storage chamber, and the other end of the first pipeline is intersected with the surface of the sensor body; one end of the second pipeline is intersected with the bottom end of the cavity wall of the liquid storage chamber, and the other end of the second pipeline is intersected with the surface of the sensor body;
the sensor also comprises two stop valves connected to the sensor body, wherein one stop valve is used for controlling the on-off state of the first pipeline, and the other stop valve is used for controlling the on-off state of the second pipeline;
the other end of the first pipeline is also connected with an air source pipe quick connector.
Specifically, in this scheme, the liquid storage chamber is used for holding the liquid in the biosensor, is set to include first pipeline and second pipeline on the sensor body, through the position limit of advance, export to first pipeline, second pipeline both simultaneously, can make the sensor when stock solution, the second pipeline is as feed liquor pipe, first pipeline is as the equalizing pipe; when the sensor changes liquid, the second pipeline is used as a liquid outlet pipe, and the first pipeline is used for introducing purge gas so as to realize quick liquid outlet of the liquid storage chamber. Simultaneously, in this scheme, through set up air supply pipe quick-operation joint on first pipeline, the convenient time shift that realizes the feed liquor is in order to realize the pressure equalizing with being connected of air supply pipe and air supply pipe quick-operation joint, when the liquid storage room goes out, through connecting air supply pipe in order to be arranged in introducing the sweep gas to the liquid storage room. In this scheme, all be provided with the stop valve on each first pipeline and the second pipeline and be used for controlling the break-make of corresponding pipeline through setting up, the accessible is opened two stop valves when inlet fluid and play liquid, is closed two stop valves when the stock solution to the stable volume of liquid holds in the realization reservoir.
In summary, the biosensor provided by the scheme has the characteristics of convenience and thoroughness in replacing liquid in the liquid storage chamber.
The further technical scheme is as follows:
the concrete implementation mode of the cut-off valve comprises a diaphragm body and two sealing patches arranged on the front surface of the diaphragm body, wherein the diaphragm body is also provided with a central hole penetrating through the front surface and the back surface of the diaphragm body;
the central hole is a round hole;
the sealing patches are arc-shaped sheets, and are concentric with the central hole, and the thickness of each sealing patch is: linearly increasing from one end to the other end of the self;
the two sealing patches are annularly and uniformly distributed relative to the central hole;
the back of the diaphragm body is also provided with a cushion block made of hard materials, and the cushion block is also provided with a circular through hole concentric with the central hole;
the projection of the diaphragm body towards the cushion block is within the edge of the cushion block;
the sealing patch is characterized by further comprising a threaded rod fixedly connected with the cushion block through the circular through hole, and the threaded rod penetrates through the central hole and extends towards one side where the sealing patch is located;
the sensor body is further provided with two accommodating cavities, one of the first pipeline and the second pipeline passes through one of the accommodating cavities, the other one of the first pipeline and the second pipeline passes through the other accommodating cavity, and the corresponding accommodating cavity has the following relation: two pipe orifices formed by intersecting the cavity walls of the corresponding accommodating cavities are positioned on the same plane;
the different stop valves are arranged in different accommodating cavities, each stop valve is in threaded connection with the sensor body through a threaded rod on the stop valve, one surface of each stop valve, which is provided with a sealing patch, is in positive relation with the plane of the pipe orifice on the corresponding accommodating cavity, the two sealing patches are respectively in positive relation with different pipe orifices, and in the process of rotating the threaded rod, the sealing patches are attached to the plane of the pipe orifice or are far away from the plane of the pipe orifice. In the scheme, the threaded rod is a rotating shaft when the cut-off valve rotates, the specific connection mode of the threaded rod and the cushion block can adopt interference connection, welding and the like, the outer surface of each sealing patch is used as a sealing surface of the cut-off valve, the sealing surface is opposite to the surface where the two pipe orifices are located, and as the sealing patches are linearly increased from one side to the other side, when the liquid inlet pipe needs to be cut off, the rotating shaft is rotated so that thicker points of the sealing patches are respectively contacted with different pipe orifices; when the liquid inlet pipe needs to be communicated, the rotating shaft is rotated to remove the joint relation between the sealing patch and the pipe orifice.
In conclusion, when the above block valve is used for on-off control of a first pipeline and a second pipeline of a liquid storage chamber of a biosensor, the block valve can be realized by adopting forward and reverse rotation, namely the scheme has the characteristics of simple structure and small volume, and is particularly suitable for small-volume design of the existing biosensor; meanwhile, the sealing patch is arranged to comprise a cushion block, and the cushion block can be made of a polymer plate, a metal plate and the like, so that the cushion block can be used as a supporting layer of the diaphragm body, and the diaphragm body has shape retaining capability to realize good lamination of the sealing patch and the pipe orifice in the process of rotating the diaphragm body, particularly when the sealing patch is contacted with the pipe orifice, thereby achieving the effect of sealing the pipe orifice; in the scheme, the central hole and the circular through hole form a through hole penetrating through the rotating shaft, so that when the cut-off valve is installed, one side of the threaded rod protrudes outwards relative to the front surface of the diaphragm body, and the other side protrudes outwards relative to the back surface of the cushion block, both sides of the cut-off valve are supported conveniently, and the stability of sealing performance of the sealing patch when the sealing patch rotates to a corresponding position can be effectively ensured; in the scheme, the threaded rod is used as a rotating shaft, so that the installation of the cut-off valve can be conveniently realized; preferably, when the threaded rod is screwed into the chamber wall, the rotation direction of the sealing patch on the cut-off valve moves from the thicker side of the sealing patch to the thinner side, so that the stroke of the thread and the local thickness of the sealing patch opposite to the pipe orifice are increased or reduced to be overlapped, and the cut-off valve can rapidly exert cut-off and communication control functions.
The projection of the sealing patch towards the diaphragm body falls within the edge of the diaphragm body. In this scheme, the setting of the relative position of sealed paster, diaphragm body, cushion three, the diaphragm body part in the outside of the circle that the accessible sealed paster is located realizes holding the seal in chamber: the diaphragm body can be used as a part of the cavity wall of the accommodating cavity, such as a rubber pad is adopted by the diaphragm body, and pressure is always provided between the outer side part of the diaphragm body and the biosensor when the diaphragm body is installed on the biosensor, so that the accommodating cavity is sealed while the diaphragm body is rotatable by utilizing elasticity of the diaphragm body.
As a specific implementation mode of the diaphragm body and the cushion block, the diaphragm body and the cushion block are disc-shaped, and the diaphragm body and the cushion block are concentric.
As an implementation that facilitates manufacturing and assembly, the sealing patch is adhered to the front face of the diaphragm body.
As an implementation manner for facilitating the manufacture and assembly, the back surface of the sealing patch is adhesively connected with the front surface of the cushion block.
In order to drive the stop valve to rotate conveniently, a rotating handle is further arranged on the side face of the cushion block.
As described above, the stop valve needs to be threaded through the threaded rod when in use, so that the above diaphragm body can be used to seal the gap between the central hole and the rotating shaft, the aperture of the central hole is smaller than that of the circular through hole, and the outer diameter of the threaded rod is between the inner diameter of the central hole and the inner diameter of the circular through hole. In this scheme, the part that the threaded rod passed the diaphragm body is the polished rod, and the cushion is the metal, and adopts spot welding to be connected between cushion and the threaded rod.
Each sealing patch is of a hollow structure with a sealing cavity inside. In this scheme, the sealed paster preferably sets up to the material and is the rubber, like this, sets up to each sealed paster all be hollow structure, like this, the accessible is gone into nitrogen gas etc. in sealed cavity, compares for setting up to sealed paster to be solid material, can make sealed paster can keep good elasticity for a long time to avoid sealed paster because ageing causes to cut off the function loss.
The invention has the following beneficial effects:
in the scheme, the liquid storage chamber is used for containing liquid in the biosensor, and the sensor body comprises a first pipeline and a second pipeline, and the positions of the inlet and the outlet of the first pipeline and the position of the outlet of the second pipeline are limited, so that the second pipeline is used as a liquid inlet pipe and the first pipeline is used as a pressure equalizing pipe when the sensor is used for storing liquid; when the sensor changes liquid, the second pipeline is used as a liquid outlet pipe, and the first pipeline is used for introducing purge gas so as to realize quick liquid outlet of the liquid storage chamber. Simultaneously, in this scheme, through set up air supply pipe quick-operation joint on first pipeline, the convenient time shift that realizes the feed liquor is in order to realize the pressure equalizing with being connected of air supply pipe and air supply pipe quick-operation joint, when the liquid storage room goes out, through connecting air supply pipe in order to be arranged in introducing the sweep gas to the liquid storage room. In this scheme, all be provided with the stop valve on each first pipeline and the second pipeline and be used for controlling the break-make of corresponding pipeline through setting up, the accessible is opened two stop valves when inlet fluid and play liquid, is closed two stop valves when the stock solution to the stable volume of liquid holds in the realization reservoir.
In summary, the biosensor provided by the scheme has the characteristics of convenience and thoroughness in replacing liquid in the liquid storage chamber.
Drawings
FIG. 1 is a top view of a shut-off valve in one embodiment of a biosensor for facilitating the replacement of a planting fluid according to the present invention;
FIG. 2 is a front view of a shut-off valve in one embodiment of a biosensor for facilitating fluid replacement according to the present invention;
FIG. 3 is a cross-sectional view taken along the direction A-A of FIG. 1;
FIG. 4 is a cross-sectional view of one embodiment of a biosensor for facilitating the replacement of a fluid according to the present invention.
The numbers in the figures are in turn: 1. the diaphragm body, 2, sealed paster, 3, centre bore, 4, the stem, 5, the cushion, 6, the threaded rod, 7, the sensor body, 71, the liquid storage chamber, 72, first pipeline, 73, second pipeline, 74, air supply pipe quick-operation joint, 8, the stop valve.
Detailed Description
The present invention will be described in further detail with reference to examples, but the structure of the present invention is not limited to the following examples.
Example 1:
as shown in fig. 1 to 4, the biosensor for facilitating replacement of the cultivation liquid comprises a sensor body 7 and a liquid storage chamber 71 arranged on the sensor body 7, wherein the liquid storage chamber 71 is a cavity sealed in the sensor body 7;
the sensor further comprises a first pipeline 72 and a second pipeline 73 which are arranged on the sensor body 7, one end of the first pipeline 72 is intersected with the top end of the cavity wall of the liquid storage chamber 71, and the other end of the first pipeline 72 is intersected with the surface of the sensor body 7; one end of the second pipeline 73 is intersected with the bottom end of the cavity wall of the liquid storage chamber 71, and the other end of the second pipeline 73 is intersected with the surface of the sensor body 7;
the sensor further comprises two stop valves 8 connected to the sensor body 7, wherein one stop valve 8 is used for controlling the on-off state of the first pipeline 72, and the other stop valve 8 is used for controlling the on-off state of the second pipeline 73;
an air supply quick connector 74 is also connected to the other end of the first conduit 72.
Specifically, in this embodiment, the liquid storage chamber 71 is configured to hold the liquid in the biosensor, and in this embodiment, the sensor body 7 includes a first pipeline 72 and a second pipeline 73, and the positions of the inlet and outlet of the first pipeline 72 and the second pipeline 73 are defined, so that the second pipeline 73 serves as a liquid inlet pipe and the first pipeline 72 serves as a pressure equalizing pipe when the sensor stores the liquid; when the sensor is used for liquid exchange, the second pipeline 73 serves as a liquid outlet pipe, and the first pipeline 72 is used for introducing purge gas so as to realize quick liquid outlet of the liquid storage chamber 71. Meanwhile, in the scheme, the air source pipe quick connector 74 is arranged on the first pipeline 72, so that the connection of the air source pipe and the air source pipe quick connector 74 during liquid inlet is convenient to realize pressure equalizing, and when the liquid storage chamber 71 discharges liquid, the air source pipe is connected for introducing purge gas into the liquid storage chamber 71. In this scheme, through setting up to all be provided with the block valve 8 on each first pipeline 72 and the second pipeline 73 and be used for controlling the break-make of corresponding pipeline, the accessible is opened two block valves 8 when entering liquid and play liquid, is closed two block valves 8 when the stock solution to the stable volume of liquid holds in the realization liquid storage chamber 71.
In summary, the biosensor provided by the present solution has the characteristics of convenience and thoroughness in replacing the liquid in the liquid storage chamber 71.
Example 2:
as shown in fig. 1 to 4, this embodiment is further defined on the basis of embodiment 1: as a specific implementation mode of the cut-off valve 8, the cut-off valve 8 comprises a diaphragm body 1 and two sealing patches 2 arranged on the front surface of the diaphragm body 1, and the diaphragm body 1 is also provided with a central hole 3 penetrating through the front surface and the back surface of the diaphragm body 1;
the central hole 3 is a round hole;
the sealing patches 2 are arc-shaped sheets, and each sealing patch 2 is concentric with the central hole 3, and the thickness of each sealing patch 2 is as follows: linearly increasing from one end to the other end of the self;
the two sealing patches 2 are annularly and uniformly distributed relative to the central hole 3;
a cushion block 5 made of hard materials is further arranged on the back surface of the diaphragm body 1, and a circular through hole concentric with the central hole 3 is further formed in the cushion block 5;
the projection of the diaphragm body 1 towards the cushion block 5 falls within the edge of the cushion block 5;
the sealing patch further comprises a threaded rod 6 fixedly connected with the cushion block 5 through the circular through hole, and the threaded rod 6 penetrates through the central hole 3 and extends towards the side where the sealing patch 2 is located;
the sensor body 7 is further provided with two accommodating chambers, one of the first pipeline 72 and the second pipeline 73 passes through one accommodating chamber, the other passes through the other accommodating chamber, and the corresponding accommodating chambers have the following relationship: two pipe orifices formed by intersecting the cavity walls of the corresponding accommodating cavities are positioned on the same plane;
the different stop valves 8 are arranged in different accommodating cavities, each stop valve 8 is in threaded connection with the sensor body 7 through a threaded rod 6 on the stop valve 8, one surface of each stop valve 8 provided with the sealing patch 2 is in positive relation with the plane of the pipe orifice in the corresponding accommodating cavity, the two sealing patches 2 are respectively in positive relation with different pipe orifices, and in the process of rotating the threaded rod 6, the sealing patches 2 are attached to the plane of the pipe orifice or are far away from the plane of the pipe orifice. In this scheme, the threaded rod 6 is a rotating shaft when the cut-off valve 8 rotates, the specific connection mode of the threaded rod 6 and the cushion block 5 can adopt interference connection, welding and the like, the outer surface of each sealing patch 2 is used as a sealing surface of the cut-off valve 8, the sealing surface is opposite to the surface where two pipe orifices are located, and as each sealing patch 2 is linearly increased from one side to the other side, when a liquid inlet pipe needs to be cut off, the rotating shaft is rotated to enable thicker points of each sealing patch 2 to be respectively contacted with different pipe orifices; when the liquid inlet pipe needs to be communicated, the rotating shaft is rotated to remove the fitting relation between the sealing patch 2 and the pipe orifice.
In summary, when the above shut-off valve 8 is used for controlling the on-off of the first pipeline 72 and the second pipeline 73 of the liquid storage chamber 71 of the biosensor, the shut-off valve 8 can be rotated in the forward and reverse directions, that is, the scheme has the characteristics of simple structure and small volume, and is particularly suitable for the small volume design of the existing biosensor; meanwhile, the sealing patch 2 is arranged to comprise the cushion block 5, and the cushion block 5 can be made of a polymer plate, a metal plate and the like, so that the cushion block 5 can be used as a supporting layer of the diaphragm body 1, and the diaphragm body 1 has shape retaining capability to realize good fitting of the sealing patch 2 and the pipe orifice in the process of rotating the diaphragm body 1, particularly when the sealing patch 2 is contacted with the pipe orifice, thereby achieving the effect of sealing the pipe orifice; in the scheme, the central hole 3 and the circular through hole form a through hole penetrating through the rotating shaft, so that when the cut-off valve 8 is installed, when one side of the threaded rod 6 is outwards protruded relative to the front surface of the diaphragm body 1 and the other side is outwards protruded relative to the back surface of the cushion block 5, both sides of the cut-off valve 8 are conveniently supported, and the stability of the sealing performance of the sealing patch 2 when the sealing patch rotates to the corresponding position can be effectively ensured; in the scheme, the threaded rod 6 is used as a rotating shaft, so that the installation of the cut-off valve 8 can be conveniently realized; preferably, when the threaded rod 6 is screwed into the chamber wall, the rotation direction of the sealing patch 2 on the cut-off valve 8 moves from the thicker side of the sealing patch 2 to the thinner side, so that the stroke of the threads and the local thickness of the sealing patch 2 opposite to the pipe orifice are increased or reduced to be overlapped, and the cut-off valve 8 can rapidly exert cut-off and communication control functions.
In this embodiment, the first pipeline 72 and the second pipeline 73 each include a vertical section and a horizontal section, one end of the first pipeline 72 located on the surface of the sensor body 7 is located at the upper end of the sensor body 7, and one end of the second pipeline 73 located on the surface of the sensor body 7 is located at the lower end of the sensor body 7. When the sensor is manufactured, the accommodating cavity is machined from the side face of the sensor body 7, then the horizontal section is drilled from the cavity bottom of the accommodating cavity, the vertical section is drilled from the bottom face or the bottom face of the sensor body 7, and a corresponding pipeline is obtained after the horizontal section and the vertical section are intersected. The shut-off valve 8 is mounted in the corresponding accommodation chamber by the side of the sensor body 7. In this way, the horizontal segments each extend into the receiving chamber, the ends of the horizontal segments acting as the nozzles. The scheme has the characteristics of easy processing and manufacturing and convenient assembly.
The projection of the sealing patch 2 towards the diaphragm body 1 falls within the edge of the diaphragm body 1. In this scheme, the setting of the relative position of sealed paster 2, diaphragm body 1, cushion 5 three, the diaphragm body 1 part in the outside of the circle that sealed paster 2 is located, the realization is to holding the sealed of chamber: the above diaphragm body 1 can be used as a part of the cavity wall of the accommodating cavity, for example, the diaphragm body 1 adopts a rubber pad, and when the diaphragm body 1 is installed on a biosensor, pressure is always provided between the outer side part of the diaphragm body 1 and the biosensor, so that the accommodating cavity is sealed while the diaphragm body 1 is rotatable by utilizing the elasticity of the diaphragm body.
As a specific implementation mode of the diaphragm body 1 and the cushion block 5, the diaphragm body 1 and the cushion block 5 are disc-shaped, and the diaphragm body 1 and the cushion block 5 are concentric.
As an implementation for ease of manufacture and assembly, the sealing patch 2 is attached to the front face of the diaphragm body 1.
As an implementation for easy manufacture and assembly, the back surface of the sealing patch 2 is adhesively connected to the front surface of the cushion block 5.
In order to drive the stop valve 8 to rotate conveniently, a rotating handle 4 is further arranged on the side face of the cushion block 5.
As described above, the stop valve 8 needs to be threaded through the threaded rod 6 when in use, so that the above diaphragm body 1 can be used to achieve sealing of the gap between the central hole 3 and the rotating shaft, the aperture of the central hole 3 is smaller than that of the circular through hole, and the outer diameter of the threaded rod 6 is between the inner diameter of the central hole 3 and the inner diameter of the circular through hole. In this scheme, the part that threaded rod 6 passed diaphragm body 1 is the polished rod, and cushion 5 is the metal, and adopts spot welding to be connected between cushion 5 and the threaded rod 6.
Each sealing patch 2 is of a hollow structure with a sealing cavity inside. In this scheme, seal patch 2 preferably sets up to the material and is the rubber, like this, sets up to each seal patch 2 and is hollow structure, like this, the accessible is gone into nitrogen gas etc. in sealed cavity, compares in setting up to seal patch 2 and is solid material, can make seal patch 2 can keep good elasticity for a long time to avoid seal patch 2 to cause because ageing cuts the function loss.
The foregoing is a further detailed description of the invention in connection with specific preferred embodiments, and it is not intended that the invention be limited to these descriptions. Other embodiments of the invention, which are apparent to those skilled in the art to which the invention pertains without departing from its technical scope, shall be covered by the protection scope of the invention.
Claims (8)
1. The biosensor convenient for replacing the cultivation liquid comprises a sensor body (7) and a liquid storage chamber (71) arranged on the sensor body (7), and is characterized in that the liquid storage chamber (71) is a cavity sealed in the sensor body (7);
the sensor comprises a sensor body (7), and is characterized by further comprising a first pipeline (72) and a second pipeline (73) which are arranged on the sensor body (7), wherein one end of the first pipeline (72) is intersected with the top end of the cavity wall of the liquid storage chamber (71), and the other end of the first pipeline (72) is intersected with the surface of the sensor body (7); one end of the second pipeline (73) is intersected with the bottom end of the cavity wall of the liquid storage chamber (71), and the other end of the second pipeline (73) is intersected with the surface of the sensor body (7);
the sensor also comprises two stop valves (8) connected to the sensor body (7), wherein one stop valve (8) is used for controlling the on-off state of the first pipeline (72), and the other stop valve (8) is used for controlling the on-off state of the second pipeline (73);
the other end of the first pipeline (72) is also connected with an air source pipe quick connector (74);
the stop valve (8) comprises a diaphragm body (1) and two sealing patches (2) arranged on the front surface of the diaphragm body (1), and a central hole (3) penetrating through the front surface and the back surface of the diaphragm body (1) is also formed in the diaphragm body (1);
the central hole (3) is a round hole;
the sealing patches (2) are arc-shaped sheets, each sealing patch (2) is concentric with the central hole (3), and the thickness of each sealing patch (2) is as follows: linearly increasing from one end to the other end of the self;
the two sealing patches (2) are annularly and uniformly distributed relative to the central hole (3);
a cushion block (5) which is made of hard material is further arranged on the back surface of the diaphragm body (1), and a circular through hole concentric with the central hole (3) is further arranged on the cushion block (5);
the projection of the diaphragm body (1) towards the cushion block (5) falls into the edge of the cushion block (5);
the sealing patch also comprises a threaded rod (6) fixedly connected with the cushion block (5) through the circular through hole, and the threaded rod (6) penetrates through the central hole (3) and extends towards one side where the sealing patch (2) is located;
the sensor body (7) is further provided with two accommodating cavities, one of the first pipeline (72) and the second pipeline (73) passes through one accommodating cavity, the other one passes through the other accommodating cavity, and the corresponding accommodating cavity has the following relation: two pipe orifices formed by intersecting the cavity walls of the corresponding accommodating cavities are positioned on the same plane;
the two sealing patches (2) are respectively in positive relation with different pipe orifices, and in the process of rotating the threaded rod (6), the sealing patches (2) are attached to or far away from the plane of the pipe orifice in the process of rotating the threaded rod (6);
the part of the threaded rod (6) penetrating through the diaphragm body (1) is a polish rod.
2. The biosensor of claim 1, wherein the projection of the sealing patch (2) towards the membrane body (1) is within the edge of the membrane body (1).
3. The biosensor for facilitating replacement of a culture solution according to claim 2, wherein the membrane body (1) and the cushion block (5) are both disc-shaped, and the membrane body (1) and the cushion block (5) are concentric.
4. The biosensor for facilitating replacement of a culture solution according to claim 1, wherein the sealing patch (2) is adhered to the front surface of the membrane body (1).
5. The biosensor for facilitating the replacement of a culture solution according to claim 1, wherein the back surface of the sealing patch (2) is adhesively connected with the front surface of the cushion block (5).
6. The biosensor for facilitating the replacement of a liquid culture according to any one of claims 1 to 5, wherein a stem (4) is further provided on a side surface of the pad (5).
7. The biosensor for facilitating replacement of a culture solution according to any one of claims 1 to 5, wherein the hole diameter of the central hole (3) is smaller than the hole diameter of the circular through hole, and the outer diameter of the threaded rod (6) is between the inner diameter of the central hole (3) and the inner diameter of the circular through hole.
8. The biosensor for facilitating the replacement of a liquid culture according to any one of claims 1 to 5, wherein each sealing patch (2) has a hollow structure with a sealing cavity provided therein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711350385.4A CN108061811B (en) | 2017-12-15 | 2017-12-15 | Biosensor convenient for cultivating liquid replacement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711350385.4A CN108061811B (en) | 2017-12-15 | 2017-12-15 | Biosensor convenient for cultivating liquid replacement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108061811A CN108061811A (en) | 2018-05-22 |
| CN108061811B true CN108061811B (en) | 2023-05-23 |
Family
ID=62139009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711350385.4A Active CN108061811B (en) | 2017-12-15 | 2017-12-15 | Biosensor convenient for cultivating liquid replacement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108061811B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001336655A (en) * | 2000-05-29 | 2001-12-07 | Matsushita Electric Ind Co Ltd | Shutoff valve |
| CN101071113A (en) * | 2007-06-12 | 2007-11-14 | 上海理工大学 | Reaction chamber and detecting chamber device for calorimetric biosensor |
| EP2100856A1 (en) * | 2008-03-11 | 2009-09-16 | biowaba GmbH | Method for microbiological treatment of water from flowing and/or standing water |
| CN102134143A (en) * | 2011-01-28 | 2011-07-27 | 北京市水利科学研究所 | Method and device for treating sewage in anaerobic bio-filter and solar aeration bio-filter |
| CN202125639U (en) * | 2011-04-14 | 2012-01-25 | 浙江武荣阀门有限公司 | Full-flow wafer check valve |
| CN102701543A (en) * | 2012-06-28 | 2012-10-03 | 天津工业大学 | Water treatment device combining microbial fuel cells with membrane technology |
| CN102749118A (en) * | 2012-07-24 | 2012-10-24 | 哈尔滨奥松机器人科技有限公司 | Liquid volume sensor |
| CN104045151A (en) * | 2014-06-26 | 2014-09-17 | 清华大学 | In-situ methane purifying reactor based on bioelectrochemical principle and in-situ methane purifying method |
| CN204696187U (en) * | 2015-07-05 | 2015-10-07 | 南通瑞泰电子有限公司 | A kind of high efficiency electrolyte injection device |
| CN207764201U (en) * | 2017-12-15 | 2018-08-24 | 重庆晓微城企业孵化器有限公司 | A kind of biosensor |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2491284B1 (en) * | 2009-10-19 | 2015-06-17 | Parker-Hannifin Corporation | Seal assembly |
-
2017
- 2017-12-15 CN CN201711350385.4A patent/CN108061811B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001336655A (en) * | 2000-05-29 | 2001-12-07 | Matsushita Electric Ind Co Ltd | Shutoff valve |
| CN101071113A (en) * | 2007-06-12 | 2007-11-14 | 上海理工大学 | Reaction chamber and detecting chamber device for calorimetric biosensor |
| EP2100856A1 (en) * | 2008-03-11 | 2009-09-16 | biowaba GmbH | Method for microbiological treatment of water from flowing and/or standing water |
| CN102134143A (en) * | 2011-01-28 | 2011-07-27 | 北京市水利科学研究所 | Method and device for treating sewage in anaerobic bio-filter and solar aeration bio-filter |
| CN202125639U (en) * | 2011-04-14 | 2012-01-25 | 浙江武荣阀门有限公司 | Full-flow wafer check valve |
| CN102701543A (en) * | 2012-06-28 | 2012-10-03 | 天津工业大学 | Water treatment device combining microbial fuel cells with membrane technology |
| CN102749118A (en) * | 2012-07-24 | 2012-10-24 | 哈尔滨奥松机器人科技有限公司 | Liquid volume sensor |
| CN104045151A (en) * | 2014-06-26 | 2014-09-17 | 清华大学 | In-situ methane purifying reactor based on bioelectrochemical principle and in-situ methane purifying method |
| CN204696187U (en) * | 2015-07-05 | 2015-10-07 | 南通瑞泰电子有限公司 | A kind of high efficiency electrolyte injection device |
| CN207764201U (en) * | 2017-12-15 | 2018-08-24 | 重庆晓微城企业孵化器有限公司 | A kind of biosensor |
Non-Patent Citations (2)
| Title |
|---|
| Magdalena A. Czekalska.An Automated Microfluidic System for the Generation of Droplet Interface Bilayer Networks.《Micromachines》.2017,第1-11页. * |
| 吴锋.低成本单室微生物燃料电池型BOD 传感器的研制.《环境科学》.2009,第30卷(第10期),第3109-3103页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108061811A (en) | 2018-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2011343777B2 (en) | pH sensor integration to single use bioreactor/mixer | |
| JPS60218059A (en) | Device and method of determining analyte in liquid | |
| US11608484B2 (en) | Single-use connection device | |
| SE448881B (en) | APPARATUS FOR PERFUSION CULTIVATION OF ONE OR MULTIPLE CELL CULTURES AND PROCEDURE FOR PERFUSION CULTIVATION OF ONE OR MULTIPLE CELL CULTURES | |
| US11307169B2 (en) | PH sensor with automatic water storage and replenishment | |
| CN108061811B (en) | Biosensor convenient for cultivating liquid replacement | |
| US10822581B2 (en) | Immobilized reaction device and a method for carrying out a reaction by utilizing the immobilization technology | |
| US20230065786A1 (en) | System for deep-sea planktonic microorganism in-situ concentration, temperature maintaining and pressure maintaining sampling | |
| CN107907647B (en) | Cut-off device for controlling on-off state of liquid storage cavity of biosensor | |
| CN207764201U (en) | A kind of biosensor | |
| KR100511052B1 (en) | Ion sensor and biochemical automatic analyzer | |
| CN116694465A (en) | Cell culture device capable of monitoring cell culture liquid environment | |
| US20170166852A1 (en) | Single-use bioreactor sensor interface | |
| CN207764180U (en) | A kind of circulation passage on off operating mode control device | |
| EP2816348A1 (en) | Electrochemical measurement device | |
| CN207764202U (en) | Cutting device for biosensor liquid storage cylinder fluid circuit | |
| CN212159653U (en) | Seawater BOD on-line monitor by microbial film method | |
| CN211652664U (en) | Dual-channel microbial sensor type BOD rapid determinator for emergency detection | |
| CN211978896U (en) | Device for rapidly obtaining maximum breathing rate of activated sludge | |
| CN207764243U (en) | Biosensor infusion circuit switchs | |
| CN220154339U (en) | Biological detection flowing electrolytic cell | |
| JP2003202315A (en) | Diaphragm cartridge | |
| CN211652665U (en) | Microbial sensor detection assembly for BOD rapid determinator | |
| CN207780028U (en) | A kind of liquid storage structure for biosensor | |
| Hilmer et al. | A new version of an in situ sampling system for bioprocess analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |