CN108060177A - A kind of Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier and preparation method thereof - Google Patents
A kind of Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier and preparation method thereof Download PDFInfo
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- CN108060177A CN108060177A CN201711478486.XA CN201711478486A CN108060177A CN 108060177 A CN108060177 A CN 108060177A CN 201711478486 A CN201711478486 A CN 201711478486A CN 108060177 A CN108060177 A CN 108060177A
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Abstract
The invention discloses a kind of Cellulose nanocrystal polyethylene imine nanometer Complex Gene carriers and preparation method thereof, interaction is occurred to combine closely by electrostatic attraction including Cellulose nanocrystal kernel and the polyethyleneimine shell being coated on outside kernel, Cellulose nanocrystal kernel and polyethyleneimine shell.The present invention genophore have good biocompatibility and high gene transfection efficiency, and reduce carrier poison, can recycle, can reduce gene transfection into preparation method is simple, at low cost.
Description
Technical field
The present invention relates to technical field of biological material, more particularly to a kind of Cellulose nanocrystal-polyethylene imine nanometer compound
Genophore and preparation method thereof.
Background technology
Genophore is a kind of instrument by gene delivery into cell, and main function is that delivery target gene enters host
Cell simultaneously successfully can be replicated and expressed, and be widely used in biological medicine, cell engineering, enzyme engineering, molecular biosciences
Etc. fields.Polyethyleneimine (PEI) is applied to biomedical research as cationic gene delivery vector, but PEI exists
Cytotoxicity is also increased while improving efficiency gene transfection, therefore its application in vivo is restricted.It is how real
The less toxic high efficiency gene transfection of existing PEI becomes a great problem.
Cellulose nanocrystal be a kind of preparation process it is simple, it is cheap, obtain convenient bioabsorbable polymer material, to environment friend
It is good, degradable and renewable, it is mainly used in high-performance composite materials.Cellulose nanocrystal has high intensity, high-crystallinity
Etc. characteristics, it is modified, compound with other nano materials by carrying out surface to it, its more performance can be assigned, this but also
Cellulose nanocrystal has huge potential and application prospect in many fields.
The content of the invention
It is an object of the invention to overcome shortcoming in the prior art, a kind of Cellulose nanocrystal-polyethylene is provided
Imine nanometer Complex Gene carrier makes genophore have good biocompatibility by Cellulose nanocrystal outer shell,
By polyethyleneimine material is made to obtain high gene transfection efficiency.
Another object of the present invention is to provide above-mentioned Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier
Preparation method.
The purpose of the present invention is achieved through the following technical solutions：
A kind of Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier, including Cellulose nanocrystal kernel with
And the polyethyleneimine shell outside kernel is coated on, Cellulose nanocrystal kernel and polyethyleneimine shell are sent out by electrostatic attraction
Life interacts and combines closely.Wherein, the surface of Cellulose nanocrystal carries negative electrical charge, and the surface of polyethyleneimine is with just
Charge, therefore the two can form electrostatic interaction.
The preparation method of above-mentioned Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier, comprises the following steps：
(1) polyethyleneimine (molecular weight 25kDa-750kDa) and 1- pi-allyl -3- methylimidazolium chlorides are added in
Reaction vessel is sufficiently mixed, and is then added in reaction vessel Cellulose nanocrystal, is stirred to react, and acquisition Cellulose nanocrystal-
Polyethyleneimine amine blends；
(2) epoxychloropropane is added in into Cellulose nanocrystal-polyethyleneimine amine blends, be stirred to react, obtain fiber
Plain nanocrystalline-polyethylene imine nanometer Complex Gene carrier.
In step (1), the mass ratio of polyethyleneimine and 1- pi-allyl -3- methylimidazolium chlorides is 1:(20~100),
Polyethyleneimine and the mass ratio of Cellulose nanocrystal are 1:(0.5~5).
In step (1), well-mixed temperature is 30~100 DEG C；Time is 1~60min.
In step (2), the molar ratio of polyethyleneimine and epoxychloropropane is 1:(0.5~3).
In step (2), the temperature being stirred to react is 60~95 DEG C；Time is 1~600min.
The present invention has the following advantages that compared with prior art and effect：
(1) genophore of the invention has good biocompatibility and high gene transfection efficiency, and reduces carrier poison
Property.
(2) genophore of the invention can recycle, and can reduce the cost of gene transfection.
(3) preparation method of the invention is simple, at low cost.
Description of the drawings
Fig. 1 is the structure diagram of Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier.
Specific embodiment
Further detailed description is done to the present invention with reference to embodiment, but the implementation of the present invention is not limited to this.
Embodiment 1
As shown in Figure 1, a kind of Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier, receives including cellulose
The transgranular core of rice and the polyethyleneimine shell being coated on outside kernel, Cellulose nanocrystal kernel and polyethyleneimine shell are close
Cooperation, kernel and shell use different materials：The inner nuclear material is Cellulose nanocrystal, and the sheathing material is poly-
Aziridine.
By polyethyleneimine (molecular weight 25kDa), 1- pi-allyl -3- methylimidazolium chlorides in mass ratio 1：20 add in
Reaction vessel, be sufficiently mixed at a temperature of 60 DEG C 1 it is small when, by Cellulose nanocrystal (with polyethyleneimine mass ratio be 0.5:
1) add in reaction vessel, be stirred to react acquisition Cellulose nanocrystal-polyethyleneimine amine compound.0.5 is added in the compound
The epoxychloropropane of poly- acetimide molar ratio again, is stirred to react 600min at a temperature of 95 DEG C, obtain Cellulose nanocrystal-
Polyethylene imine nanometer Complex Gene carrier.
Embodiment 2
By polyethyleneimine (molecular weight 750kDa), 1- pi-allyl -3- methylimidazolium chlorides in mass ratio 1:100 add
Enter reaction vessel, be sufficiently mixed at a temperature of 60 DEG C 1 it is small when, by Cellulose nanocrystal (with polyethyleneimine mass ratio be 5:
1) add in reaction vessel, be stirred to react acquisition Cellulose nanocrystal-polyethyleneimine amine compound.0.5 is added in the compound
The epoxychloropropane of poly- acetimide molar ratio again, is stirred to react 240min at a temperature of 95 DEG C, obtain Cellulose nanocrystal-
Polyethylene imine nanometer Complex Gene carrier.
Embodiment 3
By polyethyleneimine (molecular weight 100kDa), 1- pi-allyl -3- methylimidazolium chlorides in mass ratio 1:50 add
Enter reaction vessel, be sufficiently mixed at a temperature of 60 DEG C 1 it is small when, by Cellulose nanocrystal (with polyethyleneimine mass ratio be 1:
1) add in reaction vessel, be stirred to react acquisition Cellulose nanocrystal-polyethyleneimine amine compound.2.5 are added in the compound
The epoxychloropropane of poly- acetimide molar ratio again, is stirred to react 180min at a temperature of 85 DEG C, obtain Cellulose nanocrystal-
Polyethylene imine nanometer Complex Gene carrier.
Embodiment 4
By polyethyleneimine (molecular weight 50kDa), 1- pi-allyl -3- methylimidazolium chlorides in mass ratio 1:40 add in
Reaction vessel, be sufficiently mixed at a temperature of 60 DEG C 1 it is small when, by Cellulose nanocrystal (with polyethyleneimine mass ratio be 0.8:
1) add in reaction vessel, be stirred to react acquisition Cellulose nanocrystal-polyethyleneimine amine compound.1.5 are added in the compound
The epoxychloropropane of poly- acetimide molar ratio again, is stirred to react 60min at a temperature of 75 DEG C, obtain Cellulose nanocrystal-
Polyethylene imine nanometer Complex Gene carrier.
Embodiment 5
By polyethyleneimine (molecular weight 25kDa), 1- pi-allyl -3- methylimidazolium chlorides in mass ratio 1:80 add in
Reaction vessel, be sufficiently mixed at a temperature of 60 DEG C 1 it is small when, by Cellulose nanocrystal (with polyethyleneimine mass ratio be 1.2:
1) add in reaction vessel, be stirred to react acquisition Cellulose nanocrystal-polyethyleneimine amine compound.1 times is added in the compound
The epoxychloropropane of poly- acetimide molar ratio is stirred to react 30min, acquisition Cellulose nanocrystal-poly- at a temperature of 65 DEG C
Aziridine nano-complex genophore.
Claims (6)
1. a kind of Cellulose nanocrystal-polyethylene imine nanometer Complex Gene carrier, it is characterised in that：Including cellulose nanometer
Transgranular core and the polyethyleneimine shell being coated on outside kernel, Cellulose nanocrystal kernel and polyethyleneimine shell pass through quiet
Electric attraction occurs interaction and combines closely.
2. a kind of preparation method of Cellulose nanocrystal described in claim 1-polyethylene imine nanometer Complex Gene carrier,
It is characterized by comprising following step：
(1) polyethyleneimine and 1- pi-allyl -3- methylimidazolium chlorides are added in into reaction vessel, are sufficiently mixed, then by fibre
In the nanocrystalline addition reaction vessel of dimension element, it is stirred to react, obtains Cellulose nanocrystal-polyethyleneimine amine blends；
(2) epoxychloropropane is added in into Cellulose nanocrystal-polyethyleneimine amine blends, be stirred to react, obtained cellulose and receive
Meter Jing-polyethylene imine nanometer Complex Gene carrier.
3. the preparation method of Cellulose nanocrystal according to claim 2-polyethylene imine nanometer Complex Gene carrier,
It is characterized in that：In step (1), the mass ratio of polyethyleneimine and 1- pi-allyl -3- methylimidazolium chlorides is 1:(20~
100), polyethyleneimine and the mass ratio of Cellulose nanocrystal are 1:(0.5~5).
4. the preparation method of Cellulose nanocrystal according to claim 2-polyethylene imine nanometer Complex Gene carrier,
It is characterized in that：In step (1), well-mixed temperature is 30~100 DEG C；Time is 1~60min.
5. the preparation method of Cellulose nanocrystal according to claim 2-polyethylene imine nanometer Complex Gene carrier,
It is characterized in that：In step (2), the molar ratio of polyethyleneimine and epoxychloropropane is 1:(0.5~3).
6. the preparation method of Cellulose nanocrystal according to claim 2-polyethylene imine nanometer Complex Gene carrier,
It is characterized in that：In step (2), the temperature being stirred to react is 60~95 DEG C；Time is 1~600min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969757A (en) * | 2018-07-11 | 2018-12-11 | 浙江大学 | Load the cerium oxide nano composite hydrogel and preparation method and application of microRNA |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111378A (en) * | 2015-09-29 | 2015-12-02 | 赵迎辉 | Cationic polymer grafting modified nanocrystalline cellulose and preparation method and application thereof |
-
2017
- 2017-12-29 CN CN201711478486.XA patent/CN108060177A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111378A (en) * | 2015-09-29 | 2015-12-02 | 赵迎辉 | Cationic polymer grafting modified nanocrystalline cellulose and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| GAUTIER M.A. NDONG NTOUTOUME等: ""PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents—Synthesis, physicochemical characterization and in vitro evaluation"", 《CARBOHYDRATE POLYMERS》 * |
| 李宏振等: ""聚乙烯亚胺-纤维素的合成及对胆红素吸附性能"", 《林产化学与工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969757A (en) * | 2018-07-11 | 2018-12-11 | 浙江大学 | Load the cerium oxide nano composite hydrogel and preparation method and application of microRNA |
| CN108969757B (en) * | 2018-07-11 | 2021-07-27 | 浙江大学 | MicroRNA-loaded cerium oxide nano composite hydrogel and preparation method and application thereof |
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