CN108056024B - A kind of Herba Diploprora championii sprouting and rooting method - Google Patents
A kind of Herba Diploprora championii sprouting and rooting method Download PDFInfo
- Publication number
- CN108056024B CN108056024B CN201810142286.5A CN201810142286A CN108056024B CN 108056024 B CN108056024 B CN 108056024B CN 201810142286 A CN201810142286 A CN 201810142286A CN 108056024 B CN108056024 B CN 108056024B
- Authority
- CN
- China
- Prior art keywords
- culture
- pod
- seedling
- diploprora
- championii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention provides a kind of method of Herba Diploprora championii sprouting and rooting, belong to orchid germ plasm resource regeneration field, acquire Herba Diploprora championii capsule, aseptically carry out bud induction, proliferation, which is taken root, is completed at the same time Fiber differentiation, obtain the Herba Diploprora championii seedling that can be transplanted, the two step seedling methods of orchid, it is long to solve orchid cultivation cycle, it is difficult that equal productions problem at high cost solves some precious Cymbidium source resources in the prior art simultaneously, it is born in 250-1450 meters of height above sea level of mountainous region woods on trunk or is required on cheuch rock and to environmental condition harsh, wild resource is few, appreciation effect is good, naturally ability is multiplied, Spreading and diffusion is indifferent, natural renovation is difficult, the problems such as good seed is rare, it is Chinese Second Class Key Protected Plant, gardening ornamental value with higher, for orchid gardens decoration and orchid park decoration tool It is significant.
Description
Technical field
The invention belongs to orchid germ plasm resource regeneration fields, and in particular to a kind of method of Herba Diploprora championii sprouting and rooting.
Background technique
Herba Diploprora championii (scientific name: Diploprora championii (Lindl.) Hook. f.): stem is hard, cylindrical
Or slightly flat cylinder, it is often sagging.Leaf papery, sickle shaped lanceolar or plagioclase are round, and base portion has a sheath harbored, edge wave sometimes
Shape.Raceme and leaf are to life, longer than leaf or short, sagging, 2-5 flower of tool；Flower tool fragrance, slightly meat, carry out, sepal and petal
It is faint yellow；Petal is smaller than sepal；Lip white band rosiness, middle part are in boat-shaped with lower recess, without away from, be about 10 millimeters, it is wide
About 4 millimeters, slightly 3 split.Capsule is cylindrical.The month at florescence 2-8, the fruiting period 3-9 month.
It is born in 250-1450 meters of height above sea level of mountainous region woods on trunk or on cheuch rock.It is distributed in China, Sri Lanka, print
Spend (Deccan Traps), Sillim, Burma, Thailand, Vietnam.This flower has cultivation, horticultural importance with higher.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of Herba Diploprora championii sprouting and rooting, solve orchid cultivation cycle
It is long, it is at high cost that productions problem is waited to solve some precious Cymbidium source resources difficulties in the prior art simultaneously, it is born in height above sea level 250-
Harshness is required in 1450 meters of mountainous region woods on trunk or on cheuch rock and to environmental condition, wild resource is few, and appreciation effect is good,
Naturally the problems such as ability, Spreading and diffusion are indifferent, and natural renovation is difficult, good seed is rare is multiplied, is national second class protection
Plant, gardening ornamental value with higher are of great significance for orchid gardens decoration and orchid park decoration.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of Herba Diploprora championii sprouting and rooting method, includes the following steps:
1) material selection and disinfection: the mature pod detergent rinsed clean of picking current year 90%, then be placed in flowing water and rinse
20 minutes；Seed disinfection processing: pod is cleaned, and is set in saturation bleaching powder supernatant and is impregnated 15min, and with banister brush brush
It washes outside pod, after rinsing well, drop rushes 0.5h under tap water, and distilled water dashes 2-3 times, sets in superclean bench with 75% wine
Alcohol is removed after essence disinfection 30s, 0.1% mercuric chloride is added and handles 5-8min, mercury solution is poured into useless mercury bottle, sterile water rushes 3-4 after,
After blotting surface moisture with sterilized filter paper, for use；
2) bud inducement cultivation: take it is full it is sterile-processed after pod, clamp pod with gun-shaped forceps, scalpel is along pod
Vertical direction is splitted, and is taken out seed in pod, is inoculated in respectively through in high temperature, autoclaved bud inducement cultivation base；Cultivate item
Part: culture room temperature is 23 ± 2 DEG C, and after no optical culture 69d, 3h, intensity of illumination 500lx is arranged in 70d light application time, later
It is 80d that 10d light application time 5h, intensity of illumination 500-1000lx, incubation time 80d(, which all sprout), the life directly induced
Long plant；
3) proliferation, which is taken root, is completed at the same time culture: will be through above-mentioned Fiber differentiation, acquired well-grown clump bud is according to plant
Size carry out plant division inoculation, high 0.3-0.5cm, blade 1-2 piece plantlet be inoculated in bud proliferated culture medium, high about 0.5-
1.0cm, blade 2-3 piece big plant be inoculated in complete rooting process culture medium in, light application time 8h/d, intensity of illumination
1500-2000lx, Multiplying culture time 35-45d obtain being proliferated to take root for being completed at the same time growing seedlings；
4) culture of test tube bottle seedling is completed: when culture of rootage test tube seedling grows supreme 5-6cm, root 3-5 item, leaf 3-5 piece, leaf is long
The germination of Herba Diploprora championii Seed inducement and quickly breeding culture are completed when 1.5-2.5cm；
6) culture of rootage test tube bottle seedling will be completed and is placed on natural light lower refining seedling 5-7 days, bottle cap hardening 1-2d is opened, with enhancing
Adaptability of the test tube seedling to outdoor environment；Then it is taken out from culture bottle, cleans the remaining medium for being attached to root system, moved into
Fertile soil: water plant mass ratio is in the matrix of 1:2 mixing, and moisturizing shading, temperature is controlled at 15-30 DEG C, and humidity is maintained at 75%-
85%, direct sunlight is avoided,；Plant adaptability gradually increases after 2-3 weeks, obtains the complete seedling of nature robust growth.
The bud inducement cultivation base are as follows: MS+1.0mg/L spends No. 1+0.5mg/LNAA+1.0g/L peptone+20g/L of treasured
+ 1.0 gL of sucrose+5.8g/L agar powder-1Active carbon；PH value is 5.4-5.7.
The bud proliferated culture medium: 1/2MS+1.0g/L spends precious No. 2+0.5mg/LKT+0.1mg/LNAA+25g/L sugarcanes
Sugar+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.4-5.7.
The culture medium of the rooting process is that 1/2MS+1.0g/L spends No. 2+0.5mg/LKT+0.1mg/LNAA+25g/ of treasured
L sucrose+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.4-5.7.
The present invention has the advantages that
1, Establishing material selects: picking current year maturity is the 90% full pod that do not crack；2, when correctly sterilizing
Between and material processing method；3, accurate reasonable medium component is constituted；4, the setting of reasonable light application time intensity of illumination,
Improve germination rate；5, proliferation, which is taken root, is completed at the same time culture, and root induction rate is up to 100%, and survival rate is up to 95% or more；Culture
Time shortens high survival rate after seedling transplanting, and pest and disease damage is few, reduces great amount of cost, and seedling stalwartness is tall and straight, grows fine, neatly
Unanimously.
Detailed description of the invention
Fig. 1 is the bud plant after Fiber differentiation.
The seedling being completed at the same time after Fig. 2 proliferation culture of rootage.
Specific embodiment
Embodiment 1
1) material selection and disinfection: the mature pod detergent rinsed clean of picking current year 90%, then be placed in flowing water and rinse
20 minutes；Seed disinfection processing: pod is cleaned, and is set in saturation bleaching powder supernatant and is impregnated 15min, and with banister brush brush
It washes outside pod, after rinsing well, drop rushes 0.5h under tap water, and distilled water dashes 3 times, sets in superclean bench with 75% alcohol
Alcohol is removed after disinfection 30s, 0.1% mercuric chloride is added and handles 6min, mercury solution is poured into useless mercury bottle, after sterile water rushes 4 times, with disinfection
After filter paper blots surface moisture, for use；
2) bud inducement cultivation: take it is full it is sterile-processed after pod, clamp pod with gun-shaped forceps, scalpel is along pod
Vertical direction is splitted, and is taken out seed in pod, is inoculated in respectively through in high temperature, autoclaved bud inducement cultivation base；Cultivate item
Part: culture room temperature is 23 ± 2 DEG C, and after no optical culture 69d, 3h is arranged in 70d, light application time, later 10d light application time 5h,
Intensity of illumination 800lx, incubation time 80d, the growth plant directly induced；
3) proliferation, which is taken root, is completed at the same time culture: will be through above-mentioned Fiber differentiation, acquired well-grown clump bud is according to plant
Size carry out plant division inoculation, high 0.3-0.5cm, blade 1-2 piece plantlet be inoculated in bud proliferated culture medium, high 0.5-
1.0cm, blade 2-3 piece big plant be inoculated in complete rooting process culture medium in, light application time 8h/d, intensity of illumination
1800lx, Multiplying culture time 40d obtain being proliferated to take root for being completed at the same time growing seedlings；
4) culture of test tube bottle seedling is completed: when culture of rootage test tube seedling grows supreme 6cm, root 4, and 4, leaf, when the long 2cm of leaf
Complete the germination of Herba Diploprora championii Seed inducement and quickly breeding culture；
6) culture of rootage test tube bottle seedling will be completed and is placed on natural light lower refining seedling 6 days, bottle cap hardening 2d is opened, to enhance test tube
Adaptability of the seedling to outdoor environment；Then it is taken out from culture bottle, cleans the remaining medium for being attached to root system, move into humic
Soil: water plant mass ratio is in the matrix of 1:2 mixing, and moisturizing shading, temperature is controlled at 25 DEG C, and humidity is maintained at 80%, avoids sun
Light direct beam,；Plant adaptability gradually increases after 3 weeks, obtains the complete seedling of nature robust growth.
The bud inducement cultivation base are as follows: MS+1.0mg/L spends No. 1+0.5mg/LNAA+1.0g/L peptone+20g/L of treasured
+ 1.0 gL of sucrose+5.8g/L agar powder-1Active carbon；PH value is 5.6.
The bud proliferated culture medium: 1/2MS+1.0g/L spends precious No. 2+0.5mg/LKT+0.1mg/LNAA+25g/L sugarcanes
Sugar+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.6.
The culture medium of the rooting process is that 1/2MS+1.0g/L spends No. 2+0.5mg/LKT+0.1mg/LNAA+25g/ of treasured
L sucrose+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.6.
Survival rate reaches 100%.
Embodiment 2
1) material selection and disinfection: the mature pod detergent rinsed clean of picking current year 90%, then be placed in flowing water and rinse
20 minutes；Seed disinfection processing: pod is cleaned, and is set in saturation bleaching powder supernatant and is impregnated 15min, and with banister brush brush
It washes outside pod, after rinsing well, drop rushes 0.5h under tap water, and distilled water dashes 2 times, sets in superclean bench with 75% alcohol
Alcohol is removed after disinfection 30s, 0.1% mercuric chloride is added and handles 5min, mercury solution is poured into useless mercury bottle, after sterile water rushes 3 times, with disinfection
After filter paper blots surface moisture, for use；
2) bud inducement cultivation: take it is full it is sterile-processed after pod, clamp pod with gun-shaped forceps, scalpel is along pod
Vertical direction is splitted, and is taken out seed in pod, is inoculated in respectively through in high temperature, autoclaved bud inducement cultivation base；Cultivate item
Part: culture room temperature is 23 ± 2 DEG C, and after no optical culture 69d, 3h, intensity of illumination 500lx is arranged in 70d light application time, later
It is 80d that 10d light application time 5h, intensity of illumination 500-1000lx, incubation time 80d(, which all sprout), the life directly induced
Long plant；
3) proliferation, which is taken root, is completed at the same time culture: will be through above-mentioned Fiber differentiation, acquired well-grown clump bud is according to plant
Size carry out plant division inoculation, high 0.3-0.5cm, blade 1-2 piece plantlet be inoculated in bud proliferated culture medium, high about 0.5-
1.0cm, blade 2-3 piece big plant be inoculated in complete rooting process culture medium in, light application time 8h/d, intensity of illumination
1500lx, Multiplying culture time 35-45d obtain being proliferated to take root for being completed at the same time growing seedlings；
4) culture of test tube bottle seedling is completed: when culture of rootage test tube seedling grows supreme 5cm, root 3, and 3, leaf, the long 1.5cm of leaf
When complete Herba Diploprora championii Seed inducement germination and quickly breeding culture；
6) culture of rootage test tube bottle seedling will be completed and is placed on natural light lower refining seedling 5 days, bottle cap hardening 1d is opened, to enhance test tube
Adaptability of the seedling to outdoor environment；Then it is taken out from culture bottle, cleans the remaining medium for being attached to root system, move into humic
Soil: water plant mass ratio is in the matrix of 1:2 mixing, and moisturizing shading, temperature is controlled at 15 DEG C, and humidity is maintained at 75%, avoids sun
Light direct beam；Plant adaptability gradually increases after 2 weeks, obtains the complete seedling of nature robust growth.
The bud inducement cultivation base are as follows: MS+1.0mg/L spends No. 1+0.5mg/LNAA+1.0g/L peptone+20g/L of treasured
+ 1.0 gL of sucrose+5.8g/L agar powder-1Active carbon；PH value is 5.4.
The bud proliferated culture medium: 1/2MS+1.0g/L spends precious No. 2+0.5mg/LKT+0.1mg/LNAA+25g/L sugarcanes
Sugar+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.4.
The culture medium of the rooting process is that 1/2MS+1.0g/L spends No. 2+0.5mg/LKT+0.1mg/LNAA+25g/ of treasured
L sucrose+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.4.
Survival rate reaches 99%.
Embodiment 3
1) material selection and disinfection: the mature pod detergent rinsed clean of picking current year 90%, then be placed in flowing water and rinse
20 minutes；Seed disinfection processing: pod is cleaned, and is set in saturation bleaching powder supernatant and is impregnated 15min, and with banister brush brush
It washes outside pod, after rinsing well, drop rushes 0.5h under tap water, and distilled water dashes 3 times, sets in superclean bench with 75% alcohol
Alcohol is removed after disinfection 30s, 0.1% mercuric chloride is added and handles 8min, mercury solution is poured into useless mercury bottle, after sterile water rushes 4 times, with disinfection
After filter paper blots surface moisture, for use；
2) bud inducement cultivation: take it is full it is sterile-processed after pod, clamp pod with gun-shaped forceps, scalpel is along pod
Vertical direction is splitted, and is taken out seed in pod, is inoculated in respectively through in high temperature, autoclaved bud inducement cultivation base；Cultivate item
Part: culture room temperature is 23 ± 2 DEG C, and after no optical culture 69d, 3h, intensity of illumination 500lx is arranged in 70d light application time, later
10d light application time 5h, intensity of illumination 1000lx, incubation time 80d, the growth plant directly induced；
3) proliferation, which is taken root, is completed at the same time culture: will be through above-mentioned Fiber differentiation, acquired well-grown clump bud is according to plant
Size carries out plant division inoculation, and high 0.5cm, 2, blade plantlets are inoculated in bud proliferated culture medium, high about 1.0cm, blade 3
The big plant of piece is inoculated in the culture medium for completing rooting process, light application time 8h/d, intensity of illumination 2000lx, when Multiplying culture
Between 45d, obtain proliferation and take root for being completed at the same time and grow seedlings；
4) culture of test tube bottle seedling is completed: when culture of rootage test tube seedling grows supreme 6cm, root 5, and 5, leaf, the long 2.5cm of leaf
When complete Herba Diploprora championii Seed inducement germination and quickly breeding culture；
6) culture of rootage test tube bottle seedling will be completed and is placed on natural light lower refining seedling 7 days, bottle cap hardening 2d is opened, to enhance test tube
Adaptability of the seedling to outdoor environment；Then it is taken out from culture bottle, cleans the remaining medium for being attached to root system, move into humic
Soil: water plant mass ratio is in the matrix of 1:2 mixing, and moisturizing shading, temperature is controlled at 30 DEG C, and humidity is maintained at 85%, avoids sun
Light direct beam,；Plant adaptability gradually increases after 3 weeks, obtains the complete seedling of nature robust growth.
The bud inducement cultivation base are as follows: MS+1.0mg/L spends No. 1+0.5mg/LNAA+1.0g/L peptone+20g/L of treasured
+ 1.0 gL of sucrose+5.8g/L agar powder-1Active carbon；PH value is 5.7.
The bud proliferated culture medium: 1/2MS+1.0g/L spends precious No. 2+0.5mg/LKT+0.1mg/LNAA+25g/L sugarcanes
Sugar+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.7.
The culture medium of the rooting process is that 1/2MS+1.0g/L spends No. 2+0.5mg/LKT+0.1mg/LNAA+25g/ of treasured
L sucrose+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.7.
Survival rate reaches 99%.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (1)
1. a kind of Herba Diploprora championii sprouting and rooting method, characterized by the following steps:
1) material selection and disinfection: the mature pod detergent rinsed clean of picking current year 90%, then be placed in flowing water and rinse 20 points
Clock；Seed disinfection processing: pod is cleaned, and is set and is impregnated 15min in saturation bleaching powder supernatant, and scrubs pod with banister brush
Outside fruit, after rinsing well, drop rushes 0.5h under tap water, and distilled water dashes 2-3 times, sets in superclean bench and is disappeared with 75% alcohol
It removes alcohol after malicious 30s, 0.1% mercuric chloride is added and handles 5-8min, mercury solution is poured into useless mercury bottle, sterile water rushes 3-4 after, with disappearing
After malicious filter paper blots surface moisture, for use；
2) bud inducement cultivation: take it is full it is sterile-processed after pod, clamp pod with gun-shaped forceps, scalpel is vertical along pod
Direction is splitted, and is taken out seed in pod, is inoculated in respectively through in high temperature, autoclaved bud inducement cultivation base；Condition of culture: training
Supporting room temperature is 23 ± 2 DEG C, and after no optical culture 69d, 3h, intensity of illumination 500lx, later 10d illumination is arranged in 70d light application time
Time 5h, intensity of illumination 500-1000lx, incubation time 80d, the growth plant directly induced；
3) proliferation, which is taken root, is completed at the same time culture: will be through above-mentioned Fiber differentiation, acquired well-grown clump bud is according to plant size
Carry out plant division inoculation, high 0.3-0.5cm, blade 1-2 piece plantlet be inoculated in bud proliferated culture medium, high 0.5-1.0cm, leaf
The big plant of piece 2-3 piece is inoculated in the culture medium for completing rooting process, light application time 8h/d, intensity of illumination 1500-2000lx,
Multiplying culture time 35-45d obtains being proliferated to take root for being completed at the same time growing seedlings；
4) culture of test tube bottle seedling is completed: when culture of rootage test tube seedling grows supreme 5-6cm, root 3-5 item, leaf 3-5 piece, the long 1.5- of leaf
The germination of Herba Diploprora championii Seed inducement and quickly breeding culture are completed when 2.5cm；
5) culture of rootage test tube bottle seedling will be completed and is placed on natural light lower refining seedling 5-7 days, bottle cap hardening 1-2d is opened, to enhance test tube
Adaptability of the seedling to outdoor environment；Then it is taken out from culture bottle, cleans the remaining medium for being attached to root system, move into humic
Soil: water plant mass ratio is in the matrix of 1:2 mixing, and moisturizing shading, temperature is controlled at 15-30 DEG C, and humidity is maintained at 75%-
85%, avoid direct sunlight；Plant adaptability gradually increases after 2-3 weeks, obtains the complete seedling of nature robust growth；
The bud inducement cultivation base are as follows: MS+1.0mg/L spend precious No. 1+0.5mg/LNAA+1.0g/L peptone+20g/L sucrose+
+ 1.0 gL of 5.8g/L agar powder-1Active carbon；PH value is 5.4-5.7；
The bud proliferated culture medium: 1/2MS+1.0g/L spend precious No. 2+0.5mg/LKT+0.1mg/LNAA+25g/L sucrose+
5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.4-5.7；
The culture medium of the rooting process is that 1/2MS+1.0g/L spends precious No. 2+0.5mg/LKT+0.1mg/LNAA+25g/L sugarcanes
Sugar+5.6g/L agar powder+1.50gL-1Active carbon；PH value is 5.4-5.7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810142286.5A CN108056024B (en) | 2018-02-11 | 2018-02-11 | A kind of Herba Diploprora championii sprouting and rooting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810142286.5A CN108056024B (en) | 2018-02-11 | 2018-02-11 | A kind of Herba Diploprora championii sprouting and rooting method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108056024A CN108056024A (en) | 2018-05-22 |
| CN108056024B true CN108056024B (en) | 2019-08-13 |
Family
ID=62134514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810142286.5A Active CN108056024B (en) | 2018-02-11 | 2018-02-11 | A kind of Herba Diploprora championii sprouting and rooting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108056024B (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839027B (en) * | 2015-06-01 | 2016-10-05 | 福建农林大学 | A kind of Folium Agaves variegatae bulbil abductive approach |
-
2018
- 2018-02-11 CN CN201810142286.5A patent/CN108056024B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN108056024A (en) | 2018-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104604687B (en) | Utilize the method for cutting the induction Multiple Buds propagating quickly butterfly orchid of the bennet stem section after bud | |
| CN103609224B (en) | Louisiana iris hybrid seed germination method | |
| CN106417015B (en) | A kind of Huaiji primulina tabacum tissue cultures and rapid propagation method | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN101124889B (en) | Method for inducing new strain of tetraploid dendrobium with seeds protocorm | |
| CN103651141B (en) | The method that Bo chrysanthemum batch production test tube seedling is the most numerous | |
| CN101347098A (en) | Method of propagation of Tilia miqueliana by tissue culture | |
| CN108901845B (en) | In-vitro propagation method of plant water horns | |
| CN109042330A (en) | A kind of method for tissue culture of spindle tree | |
| CN108782247A (en) | A kind of method for tissue culture of late cherry " Yu Yihuang " kind of Japan | |
| CN104686344A (en) | Tissue culture method of liriope muscari | |
| CN110178726B (en) | Rooting medium for tissue culture and rapid propagation of weeping willows and tissue culture and rapid propagation method of weeping willows | |
| CN108142281A (en) | A kind of Cortex Eucommiae method for tissue culture | |
| CN108271693A (en) | One kind posting tree orchid regenerating system quickly foundation and store method | |
| CN105766636A (en) | Tissue culture regeneration method of paeonia suffruticosa | |
| CN105519445A (en) | In-vitro rapid propagation method for nepenthes | |
| CN108056024B (en) | A kind of Herba Diploprora championii sprouting and rooting method | |
| CN103858768A (en) | Tissue culture method of plumeria rubra L.cv.Acutifolia | |
| CN1110250C (en) | Fast seedling growing mehtod for Chinese flowering crabapple zhumei | |
| CN105794650A (en) | Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds | |
| CN106942065B (en) | A kind of set aspidistra in vitro culture quick-breeding method | |
| CN108552057B (en) | Method for establishing efficient recycling system of topaz | |
| CN100382677C (en) | Lily ball breeding method for fresh cutting flower production | |
| CN106888970B (en) | A kind of tissue culture and rapid propagation method of the point leaf basin away from orchid | |
| CN105340746A (en) | Method for cultivating octoploid lowland type switchgrass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |