CN108051539A - The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE - Google Patents
The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE Download PDFInfo
- Publication number
- CN108051539A CN108051539A CN201711059434.9A CN201711059434A CN108051539A CN 108051539 A CN108051539 A CN 108051539A CN 201711059434 A CN201711059434 A CN 201711059434A CN 108051539 A CN108051539 A CN 108051539A
- Authority
- CN
- China
- Prior art keywords
- solution
- sample
- ion
- anion
- citrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8624—Detection of slopes or peaks; baseline correction
- G01N30/8631—Peaks
- G01N30/8634—Peak quality criteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
- G01N2030/965—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange suppressor columns
Abstract
The invention discloses the method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE, chromatographic condition is：Chromatographic column is 11 HC of AS (250mm × 4mm), 11 HC of guard column AG (50mm × 4mm)；Leacheate is the sodium hydroxide solution of 33~35mmol/L（Logical nitrogen）；28~33 DEG C of column temperature；0.9~1.1ml/min of flow velocity；10~20 μ l of sample size；Using anion suppressor and electric conductivity detector.The method has the advantages that easy to operate, quick, accuracy is high, precision is high, stability is good, and laboratory is suitble to detect work for the technicality of XUESHUANTONG ZHUSHEYE, can be detected in batches, improves detection efficiency.
Description
Technical field
The present invention relates to drug detection fields, are specifically the method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE.
Background technology
XUESHUANTONG ZHUSHEYE and injection thrombus are completely known as XUESHUANTONG ZHUSHEYE, main component be arasaponin and
Sodium chloride.XUESHUANTONG ZHUSHEYE（Alias：Tienchi-Ginseng parenteral solution）, it is a kind of injection prescription medicine, to be faint yellow to yellow
Clear liquid suitable for thrombosis of central vein of retina, the treatment of hyphema, glaucoma, apoplexy sequela, also may be used
For treating virus hepatitis, promoting blood circulation is also equipped with, expands blood vessel, improves the effect of blood circulation, but occasionally has allergic reaction.Note
Penetrate and led to thrombus for off-white color or faint yellow amorphous powder or porous solid shape object, hide slight bitter, micro-sweet, have draw it is moist.It adapts to
Disease is promoting blood circulation, is promoted blood circulation active.For obstruction of collaterals by blood stasis, hemiplegia, chest impediment and cardialgia and thrombosis of central vein of retina disease.
The injection supplementary materials such as sodium chloride, sodium citrate, activated carbon are applied in XUESHUANTONG ZHUSHEYE, to strengthen auxiliary material
Quality control ensures the stable quality of auxiliary material, and it is highly important to carry out quality control to several anion in auxiliary material.Due to work
Property charcoal introduce impurity using activator when using chemical activation, such as phosphoric acid.Human body, which takes in too many phosphorus, can make in vivo
Calcium can not fully absorb, utilize, easily cause fracture, loss of tooth and textured bone.Long-term a large amount of intake phosphate can be led
Cause Thyroid Gland Swell, Calcified renal insufficiency etc..
Sodium chloride is added in as osmotic pressure regulator in XUESHUANTONG ZHUSHEYE, and addition is 8.5g/1000ml, that is, 8.5mg/
ml.Because sodium ion, chlorion can be by glomerular filtrations, and part is by reabsorption, by kidney with homaluria, only portion less
Divide and discharged from sweat, therefore the heart, renal insufficiency person use with caution.And water-sodium retention can cause oedema, blood pressure rise, increased heart rate, it is uncomfortable in chest,
Expiratory dyspnea or even acute left ventricular failure, it is therefore necessary to carry out quality control.
The content of the invention
The object of the present invention is to provide the method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE, which has operation
Simply, quickly, the advantages of accuracy is high, precision is high, stability is good.
The invention is realized by the following technical scheme：
The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE, three kinds of anion for chlorion, citrate from
Son and dihydrogen phosphate ions；Its detection method comprises the following steps：
（a）The preparation of reference substance solution：Take chlorion standard solution, add water be made chloride ion content be respectively 5,10,20,30,
The solution of 40 μ g/ml；Take H2PO4 -Standard solution adds water that H is made2PO4 -Ion concentration is respectively the molten of 1,2,6,8,20 μ g/ml
Liquid；Citrate ion solution is taken, it is respectively the molten of 10,20,50,100,300 μ g/ml to add water that citrate ion content is made
Liquid；
（b）The preparation of sample solution：XUESHUANTONG ZHUSHEYE is taken to be diluted with water 8~10 times, as dihydrogen phosphate ions and lemon
Acid ion sample solution；XUESHUANTONG ZHUSHEYE is taken to be diluted with water 90~100 times, as chlorion sample solution；Or take note
Solution by the use of thrombus wildcard into 3.7~4mg/ml is penetrated as sample solution；
（c）High performance liquid chromatography detects：It is accurate respectively to draw reference substance solution and each 10~20 μ l of sample solution, inject ion
Chromatograph is measured according to certain chromatographic condition, obtains chromatogram；
（d）Result is calculated：Using reference substance sample size as abscissa（X）, reference substance peak area is ordinate（Y）Drafting standard
Curve obtains regression equation；Peak area is calculated by chromatogram, sample peak area is substituted into regression equation, sample is calculated
The content of middle tannin；The content of chlorion, citrate and dihydrogen phosphate is calculated.
The method that three kinds of anion synchronously detect in the XUESHUANTONG ZHUSHEYE is analyzed using the method for ion chromatography
Detection, chromatographic condition are：Chromatographic column is AS-11-HC (250mm × 4mm), guard column AG-11-HC (50mm × 4mm)；Leaching
Washing lotion is the sodium hydroxide solution of 33~35mmol/L（Logical nitrogen）；28~33 DEG C of column temperature；0.9~1.1ml/min of flow velocity；Sample introduction
Measure 10~20 μ l；Using anion suppressor and electric conductivity detector.
The testing conditions and method that the present invention uses are on the basis of lot of documents is consulted, and are ground by many experiments exploration
Study carefully what is screened afterwards, can achieve the purpose that accuracy is high, stability is good.
Beneficial effects of the present invention：
1st, the method that three kinds of anion of tannin synchronously detect in XUESHUANTONG ZHUSHEYE of the present invention, have it is easy to operate, quick,
Accuracy is high, precision is high, stability is good, high repeatability and other advantages.
2nd, the method that three kinds of anion of tannin synchronously detect in XUESHUANTONG ZHUSHEYE of the present invention, using the chromatography of ions
It is detected, the technicality in the laboratories such as testing agency for XUESHUANTONG ZHUSHEYE is suitble to detect work, it particularly can be large quantities of
Amount detection, greatly improves detection efficiency.In addition, the method for the invention can carry for the foundation of company standard or professional standard
For important reference frame.
Description of the drawings
Fig. 1 is the chromatogram of chlorion reference substance.
Fig. 2 is the chromatogram of dihydrogen phosphate ions reference substance.
Fig. 3 is the chromatogram of citrate ion reference substance.
Fig. 4 is Chloride Solution standard curve.
Fig. 5 is dihydrogen phosphate ions solution standard curve.
Fig. 6 is citrate ion solution standard curve.
Specific embodiment
The present invention is introduced in order to more detailed, with reference to embodiment and attached drawing, the present invention will be further described.It is real
Example is applied to be not limited to the technical solution, it is all based on the modification made in scope of the invention as claimed, still exist
In scope of the presently claimed invention.
Instrument and reagent used be：Wear peace ICS-3000 ion chromatographs, Chromelen chromatographic work stations, anion
Suppressor, electric conductivity detector；MILLI-PROA pure water processors；Chromatographic column：AS-11-HC (250mm × 4mm), guard column AG-
11-HC(50mm×4mm)；Water is 18.2 M Ω；Water is deionized water, and sodium hydroxide solution is chromatographically pure；Cl-Standard solution
（China National Measuring Science Research Inst. provide, concentration be 1000 μ g/ml, lot number 10084）；H2PO4 -Standard solution（Chinese measuring science
Research institute provides, concentration 1000mg/L, lot number 1105）；Citrate ion solution（Biosmart is provided, and concentration is
1000mg/L, lot number 081711）.
Embodiment 1
The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE, three kinds of anion for chlorion, citrate from
Son and dihydrogen phosphate ions；Its detection method comprises the following steps：
（a）The preparation of reference substance solution：Take chlorion standard solution, add water be made chloride ion content be respectively 5,10,20,30,
The solution of 40 μ g/ml；Take H2PO4 -Standard solution adds water that H is made2PO4 -Ion concentration is respectively the molten of 1,2,6,8,20 μ g/ml
Liquid；Citrate ion solution is taken, it is respectively the molten of 10,20,50,100,300 μ g/ml to add water that citrate ion content is made
Liquid；
（b）The preparation of sample solution：XUESHUANTONG ZHUSHEYE is taken to be diluted with water 10 times, as dihydrogen phosphate ions and citrate
Ion samples solution；XUESHUANTONG ZHUSHEYE is taken to be diluted with water 100 times, as chlorion sample solution；
（c）High performance liquid chromatography detects：Accurate absorption reference substance solution and each 20 μ l of sample solution respectively, injecting chromatograph,
It is measured according to certain chromatographic condition, obtains chromatogram；
（d）Result is calculated：Using reference substance sample size as abscissa（X）, reference substance peak area is ordinate（Y）Drafting standard
Curve obtains regression equation；Peak area is calculated by chromatogram, sample peak area is substituted into regression equation, sample is calculated
The content of middle tannin；The content of chlorion, citrate and dihydrogen phosphate is calculated.
The method that three kinds of anion synchronously detect in the XUESHUANTONG ZHUSHEYE is analyzed using the method for ion chromatography
Detection, chromatographic condition are：Chromatographic column is AS-11-HC (250mm × 4mm), guard column AG-11-HC (50mm × 4mm)；Leaching
Washing lotion is the sodium hydroxide solution of 35mmol/L（Logical nitrogen）；30 DEG C of column temperature；Flow velocity 1.0ml/min；10 μ l of sample size；Using the moon
Suppressor ion and electric conductivity detector.
Fig. 1,2,3 are respectively the reference substance chromatogram of chlorion, citrate and dihydrogen phosphate.
It is as follows that methodology validation experiment is carried out to detection method described in embodiment：
1st, chlorion methodology validation is tested
（1）The range of linearity and calibration curve
Chlorion standard solution is taken, precision measurement 0.1,0.2,0.5,1.0,3.0ml put in 10ml measuring bottles, be diluted with water respectively
To scale, shake up.Accurate respectively to draw each 100 μ l of sample introduction of more than reference substance solution, injection ion chromatograph is measured, tests
The results are shown in Table 1.Using reference substance sample size as abscissa（X）, integrating peak areas value is ordinate（Y）Draw standard curve,
See Fig. 4, reference substance regression equation is：Y=1.1516X -0.4209, r=1.000.The result shows that：Chlorion standard solution into
It is in good linear relationship with chlorion ion peak areas when sample amount is in 1~30 μ g ranges.
1 chlorion standard curve result of table
（2）Precision test
It takes with a sample solution, by the chromatographic condition in embodiment, METHOD FOR CONTINUOUS DETERMINATION 5 times, experimental result is as shown in table 2.As a result
Show the chlorion peak area average value of 5 measure, measurement result is：RSD=1.67%（n=5）.It is experiments have shown that of the present invention
The precision of method is good.
2 Precision test result of table
（3）Repetitive test
XUESHUANTONG ZHUSHEYE is taken, by the method parallel determination 6 times in embodiment, experimental result is as shown in table 3.6 parts of samples measure
The average value of chloride ion content is 4.8774mg/ml, RSD=0.98%.Result of the test shows the reproduction of the method for the invention
Property is preferable.
3 repetitive test result of table
（4）Recovery test
Precision measures XUESHUANTONG ZHUSHEYE（Content is 4.8774mg/ml）0.25ml is put in 50ml measuring bottles, and precision adds in above-mentioned chlorine
Ion standard solution 1ml is extracted by the method in embodiment, measured, calculates sample recovery rate, and experimental result is as shown in table 4.It is real
Test the requirement for showing that this method the method meets quantitative analysis.
4 sample recovery rate result of calculation of table
（5）Stability test
It by the chromatographic condition described in embodiment, measures once, when investigation 6 is small altogether, determines 5 times at regular intervals.Experiment
The results are shown in Table 5, and as a result the average peak area of 5 measure is 55.320, RSD=0.79%.Experiments have shown that sample solution is extremely
Few interior stabilization when 6 is small.
5 stability test result of table
（6）Blank control test
In addition to sample is not weighed, carried out according to step under " preparation of sample solution " item in embodiment, blank control is made
Solution.Sample introduction measures, and as a result occurs in chlorion peak corresponding position without chromatographic peak, illustrates that blank is noiseless.
2nd, dihydrogen phosphate ions and the experiment of citrate ion methodology validation
（1）The range of linearity and calibration curve
Take H2PO4 -Standard solution, precision measure 1ml, put respectively in 50ml measuring bottles, be diluted with water to scale, shake up.Precision measures
0.5th, 1.0,3.0,4.0,10.0ml, puts in 10ml measuring bottles, is diluted with water to scale, shake up respectively.Respectively more than accurate absorption
Each 100 μ l of sample introduction of reference substance solution, injection ion chromatograph are measured, and result of the test is as shown in table 6.With reference substance sample size
For abscissa（X）, integrating peak areas value is ordinate（Y）Draw standard curve（As shown in Figure 5）, reference substance regression equation is：Y
=2.8877X -0.2841, r=0.9990.The result shows that：H2PO4 -When standard solution sample size is in 0.10~2.0 μ g ranges,
With H2PO4 -Peak area is in good linear relationship.
6 H of table2PO4 -Standard curve result
| H2PO4 -Amount（μg） | 0.10 | 0.20 | 0.60 | 0.80 | 2.00 |
| Peak area | 0.106 | 0.324 | 1.397 | 1.882 | 5.557 |
Citrate standard solution is taken, precision measures 1ml, puts respectively in 10ml measuring bottles, be diluted with water to scale, shake up.It is accurate
Measurement 0.5,1.0,2.0,3.0,4.0ml, put in 10ml measuring bottles, are diluted with water to scale, shake up respectively.Respectively it is accurate draw with
Upper each 100 μ l of sample introduction of reference substance solution, injection ion chromatograph are measured, and result of the test is as shown in table 7.With reference substance sample introduction
It measures as abscissa（X）, integrating peak areas value is ordinate（Y）Draw standard curve（As shown in Figure 6）, reference substance regression equation
For：Y=1.9258X -0.1590, r=0.9997.The result shows that：Citrate standard solution sample size is in 0.50~4.00 μ g
In the range of when, with citrate peak area be in good linear relationship.
7 citrate standard curve result of table
| Citrate amount（μg） | 0.50 | 1.00 | 2.00 | 3.00 | 4.00 |
| Peak area | 0.814 | 1.806 | 3.667 | 5.521 | 7.620 |
（2）Precision test
It takes with a sample solution, by the chromatographic condition described in embodiment, METHOD FOR CONTINUOUS DETERMINATION 5 times, experimental result is as shown in table 8.
The result shows that the citrate peak area average value of 5 measure, measurement result are：RSD=1.81%（n=5）.Experiments have shown that this hair
The precision of bright the method is good.（Note：H2PO4 -It does not detect, therefore peak area is citrate peak area）
8 Precision test result of table
| Sequence number | 1 | 2 | 3 | 4 | 5 | Average value | RSD% |
| Peak area | 3.653 | 3.675 | 3.756 | 3.774 | 3.812 | 3.734 | 1.81 |
（3）Repetitive test
XUESHUANTONG ZHUSHEYE is taken, by the method parallel determination 6 times described in embodiment, experimental result is as shown in table 9,6 parts of samples
The average value for measuring citrate ion content is 0.2191mg/ml, H2PO4 -It does not detect, content is represented with " 0mg/ml ".Examination
Test the result shows that, the reappearance of the method for the invention is preferable.
9 repetitive test result of table
| Sequence number | 1 | 2 | 3 | 4 | 5 | 6 | Average value |
| Sampling amount（ml） | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | / |
| Content（mg/ml） | 0.2030 | 0.2310 | 0.2335 | 0.2365 | 0.2315 | 0.2030 | 0.2191 |
（4）Recovery test
H2PO4 -It is prepared by standard reserving solution：Precision measures H2PO4 -Standard solution 1ml is put in 100ml measuring bottles, is diluted with water to quarter
Degree, shake up to get.
It is prepared by citrate standard reserving solution：Precision measures citrate standard solution 1.5ml, puts in 10ml measuring bottles, adds
Water is diluted to scale, shake up to get.
Precision measures XUESHUANTONG ZHUSHEYE（H2PO4 -Content is 0mg/ml, and citrate content is 0.2191mg/ml）
0.5ml is put in 10ml measuring bottles, accurate respectively to add in above-mentioned H2PO4 -Standard solution 5ml and citrate standard solution 1ml, by reality
It applies the method extraction described in example, measure, calculate sample recovery rate, experimental result is as shown in table 10,11.Experiment shows this hair
Bright the method meets the requirement of quantitative analysis.
10 H of table2PO4 -Sample recovery rate result of calculation
11 citrate sample recovery rate result of calculation of table
（5）Stability test
It by the chromatographic condition described in embodiment, measures once, when investigation 16 is small altogether, determines 5 times at regular intervals, it is real
Test that result is as shown in table 12, the average peak areas of 5 measure are 3.874, RSD=3.77%.Experiments have shown that sample solution is at least
The interior stabilization when 16 is small.
12 stability test result of table
（6）Blank control test
In addition to sample is not weighed, carried out by step under " preparation of sample solution " item described in embodiment, sky is made in accordance with the law
White contrast solution.Sample introduction measures, as a result in H2PO4 -Occur with citrate ion peak corresponding position without chromatographic peak, illustrate sky
It is white noiseless.
Claims (2)
1. the method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE, which is characterized in that three kinds of anion for chlorine from
Son, citrate ion and dihydrogen phosphate ions；Its detection method comprises the following steps：
（a）The preparation of reference substance solution：Take chlorion standard solution, add water be made chloride ion content be respectively 5,10,20,30,
The solution of 40 μ g/ml；Take H2PO4 -Standard solution adds water that H is made2PO4 -Ion concentration is respectively the molten of 1,2,6,8,20 μ g/ml
Liquid；Citrate ion solution is taken, it is respectively the molten of 10,20,50,100,300 μ g/ml to add water that citrate ion content is made
Liquid；
（b）The preparation of sample solution：XUESHUANTONG ZHUSHEYE is taken to be diluted with water 8~10 times, as dihydrogen phosphate ions and lemon
Acid ion sample solution；XUESHUANTONG ZHUSHEYE is taken to be diluted with water 90~100 times, as chlorion sample solution；Or take note
Solution by the use of thrombus wildcard into 3.7~4mg/ml is penetrated as sample solution；
（c）High performance liquid chromatography detects：It is accurate respectively to draw reference substance solution and each 10~20 μ l of sample solution, inject ion
Chromatograph is measured according to certain chromatographic condition, obtains chromatogram；
（d）Result is calculated：Using reference substance sample size as abscissa（X）, reference substance peak area is ordinate（Y）Drafting standard
Curve obtains regression equation；Peak area is calculated by chromatogram, sample peak area is substituted into regression equation, sample is calculated
The content of middle tannin；The content of chlorion, citrate and dihydrogen phosphate is calculated.
2. the method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE according to claim 1, which is characterized in that use
The method of ion chromatography carries out analysis detection, and chromatographic condition is：Chromatographic column be AS-11-HC (250mm × 4mm), guard column
AG-11-HC(50mm×4mm)；Leacheate is the sodium hydroxide solution of 33~35mmol/L（Logical nitrogen）；28~33 DEG C of column temperature；
0.9~1.1ml/min of flow velocity；10~20 μ l of sample size；Using anion suppressor and electric conductivity detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711059434.9A CN108051539A (en) | 2017-11-01 | 2017-11-01 | The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711059434.9A CN108051539A (en) | 2017-11-01 | 2017-11-01 | The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108051539A true CN108051539A (en) | 2018-05-18 |
Family
ID=62119865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711059434.9A Pending CN108051539A (en) | 2017-11-01 | 2017-11-01 | The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108051539A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101251520A (en) * | 2008-02-29 | 2008-08-27 | 浙江大学 | Double phosphonic acid salt medicaments and ion chromatographic fractionation analysis method of impurity anion thereof |
| CN102128885A (en) * | 2010-12-08 | 2011-07-20 | 广东中烟工业有限责任公司 | Method for quickly and simultaneously detecting 16 inorganic anions and organic acids in tobacco |
| CN102520098A (en) * | 2011-10-24 | 2012-06-27 | 南京大学 | Method for determining trace low-valent phosphate in natural water |
| CN103149306A (en) * | 2013-03-28 | 2013-06-12 | 中粮生物化学（安徽）股份有限公司 | Method of analyzing content of anions in citric acid-containing solution |
| CN106568886A (en) * | 2016-10-20 | 2017-04-19 | 舒泰神（北京）生物制药股份有限公司 | Method for measuring contents of cations and anions in polyethylene glycol electrolyte preparation |
-
2017
- 2017-11-01 CN CN201711059434.9A patent/CN108051539A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101251520A (en) * | 2008-02-29 | 2008-08-27 | 浙江大学 | Double phosphonic acid salt medicaments and ion chromatographic fractionation analysis method of impurity anion thereof |
| CN102128885A (en) * | 2010-12-08 | 2011-07-20 | 广东中烟工业有限责任公司 | Method for quickly and simultaneously detecting 16 inorganic anions and organic acids in tobacco |
| CN102520098A (en) * | 2011-10-24 | 2012-06-27 | 南京大学 | Method for determining trace low-valent phosphate in natural water |
| CN103149306A (en) * | 2013-03-28 | 2013-06-12 | 中粮生物化学（安徽）股份有限公司 | Method of analyzing content of anions in citric acid-containing solution |
| CN106568886A (en) * | 2016-10-20 | 2017-04-19 | 舒泰神（北京）生物制药股份有限公司 | Method for measuring contents of cations and anions in polyethylene glycol electrolyte preparation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Margolis et al. | Stabilization of ascorbic acid in human plasma, and its liquid-chromatographic measurement. | |
| CN106814150B (en) | Isotope dilution ultra-performance liquid chromatography-mass spectrometry combined vitamin K determination method1Method (2) | |
| CN104055797A (en) | Detection and identification of two composition components in toad skin extract | |
| CN108845070A (en) | A kind of efficient liquid phase detection method of L-citrulline | |
| CN109632980B (en) | Method for simultaneously detecting uridine and ergosterol and application thereof | |
| Barberi-Heyob et al. | Analysis of 5-fluorouracil in plasma and urine by high-performance liquid chromatography | |
| CN106324167B (en) | Method for determining flavonoid components in astragalus extract by UPLC | |
| CN105044248B (en) | The quantitative detecting method of natrium adetate and sodium pyrosulfite in a kind of ginseng and astragalus injection for strengthening body | |
| CN104280473B (en) | The analytical approach of 5 hydroxymethyl furfural in a kind of Linezolid Injection | |
| CN103630633A (en) | Method for measuring contents of six amino acids in ixeris sonchifolia injection by using pre-column derivatization method | |
| CN108051539A (en) | The method that three kinds of anion synchronously detect in XUESHUANTONG ZHUSHEYE | |
| CN102133333A (en) | Quality control method for shenmai injection mass spectrum finger prints | |
| CN103940918A (en) | A method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma | |
| Sun et al. | Determination of gemcitabine and its metabolite in extracellular fluid of rat brain tumor by ultra performance liquid chromatography–tandem mass spectrometry using microdialysis sampling after intralesional chemotherapy | |
| CN107102087A (en) | A kind of method of a variety of organic acid contents in chromatography of ions detection coptis | |
| CN113917008A (en) | Application of product for detecting metabolite level in urine by mass spectrometry in preparation of product for early evaluation of intestinal polyp and colorectal cancer | |
| CN107764912B (en) | Construction method of bone-strengthening injection HPLC fingerprint | |
| CN110261494A (en) | A kind of method and its detection kit for identifying thyroid malignancy biomarker | |
| KR20160004733A (en) | Method of analyzing blood marbofloxacin concentration | |
| CN109406656A (en) | A kind of method and its detection kit for identifying psoriasis biomarker | |
| CN112710773B (en) | Method for simultaneously detecting fosfomycin and diol thereof by adopting ion chromatography | |
| AK et al. | Simple and rapid method for simultaneous determination of isoniazid and acetyl isoniazid in urine by HPLC | |
| CN115326990B (en) | Method for detecting hydrogen sulfide in biological sample and application thereof | |
| CN112162049B (en) | Method for detecting sarcosine in urine for non-diagnosis purpose | |
| CN109632984B (en) | Detection method of bilobalide injection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180518 |
|
| RJ01 | Rejection of invention patent application after publication |