The content of the invention
Based on this, it is necessary to provide cell in a kind of cell culture that can efficiently and accurately measure cell viability and live
Power measurement device.
Cell viability measurement device in a kind of cell culture, including:
Light-transmission tube, the top of the light-transmission tube have the bottom lock of opening and the light-transmission tube, and the light-transmission tube includes
Fixed part positioned at top, the measurement portion positioned at bottom and the connection fixed part and the transition part of the measurement portion, institute
The internal diameter of fixed part is stated as 0.5cm-1cm, the internal diameter of the measurement portion is 400 μm -600 μm;And
Reading assembly, the reading assembly, which includes being protected from light housing and is located at, described is protected from light the in vivo fixing device of shell, hair
Electro-optical device, image forming optics, imaging sensor, processing unit, the fixing device are used to fix the light-transmission tube, the hair
Close to the fixing device for shining towards the light-transmission tube, the image forming optics fill electro-optical device with the fixation
It puts in opposite directions, the image forming optics are used to be imaged the measurement portion, and the imaging sensor is connected to the image optics
Device for receiving the image-forming information of the image forming optics, the processing unit be connected to the imaging sensor with
It is assessed in the acquisition image-forming information and according to preset rules.
In one of the embodiments, the internal diameter of the transition part by close to one end of the measurement portion to close to described solid
The one end for determining portion gradually increases.
In one of the embodiments, the outer diameter of the measurement portion is less than the outer diameter of the fixed part.
In one of the embodiments, the outer diameter of the transition part by close to one end of the measurement portion to close to described solid
The one end for determining portion gradually increases, and the light-transmission tube is in funnel-form.
In one of the embodiments, the outer wall of the light-transmission tube is connected at least a pair of of stabilizer, stablizes described in each pair
The position of two stabilizers in the wing is opposite, and the one side of each stabilizer is connected with the measurement portion, opposite side with
The transition part connection.
In one of the embodiments, the lateral wall edge of the stabilizer is extended to puts down with the outer wall of the fixed part
Together, the bottom margin of the stabilizer extends to concordant with the bottom of the light-transmission tube.
In one of the embodiments, fixing bracket is further included, there are multiple fixed card slots on the fixing bracket, it is described
Fixed card slot is adapted to the light-transmission tube.
In one of the embodiments, the fixing device, which has, reads card slot, the reading card slot and the light-transmission tube
Adaptation.
In one of the embodiments, there is the housing that is protected from light reading to be open, and described be protected from light is hingedly attached to the housing with rotation
Door, the revolving door for close or open it is described read opening, the fixing device is connected to the revolving door inwardly
On surface.
In one of the embodiments, the housing that is protected from light includes fixed plate, limiting plate and is protected from light clamshell, the /V
The quantity of plate is two, and two limiting plates are connected to the opposite both sides of the fixed plate, and two limiting plates are put down
Row, one of them described limiting plate be equipped with the revolving door, the light-emitting device, the image forming optics and it is described into
As sensor is each provided in the fixed plate, the clamshell that is protected from light is located on the fixed plate and the limiting plate.
Cell viability measurement device in above-mentioned cell culture, by putting the cell suspending liquid after dyeing in light-transmission tube
In, cell suspending liquid in light-transmission tube will be centrifuged and obtain the interior cell mass compressed of pipe, cell mass is subdivided into different height
Three parts, complete cell is deposited on since density is higher in the lower area of capillary, and cell is not colored because
The infiltration of their cell membrane tolerance dyestuff.Dead cell and cell fragment deposit uppermost region since density is higher, cell
Film is resistant to the infiltration of dyestuff, therefore region is farthest dyed.The cell viability of intermediate region is between lower area and upper
Between portion region, color gradient is formed in the intermediate region.By the fixation that the light-transmission tube after centrifuging is placed in reading assembly
On device, light-emitting device shines towards the light-transmission tube, and image forming optics are imaged the light-transmission tube, and imaging sensor receives
The image-forming information of image forming optics, the image-forming information are transferred to processing unit, and processing unit is commented according to default rule
Estimate, determine the height of cell cake, be especially to determine the height of each cell mass in sample container.
Cell viability measurement device in above-mentioned cell culture, by stabilizer is set with improve light-transmission tube movement, from
The stability during heart.
Cell viability measurement device in above-mentioned cell culture by setting fixing bracket, has multiple on fixing bracket
Fixed card slot, multiple fixed card slots can be set for multiple light transmission pipe clamps, fixing bracket may be employed adaptation centrifuge carry out from
Multiple light-transmission tubes can be inserted into centrifuge simultaneously or be taken out from centrifuge, improve the centrifugation of multiple light-transmission tubes by the heart
The uniformity for the treatment of conditions, while time power is also saved, operating efficiency is high.
Specific embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In attached drawing
Give presently preferred embodiments of the present invention.But the present invention can realize in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is with belonging to technical field of the invention
The normally understood meaning of technical staff is identical.Term used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
The arbitrary and all combination of the Listed Items of pass.
The present embodiment relates to cell viability measurement device in a kind of cell culture.Cell viability in the cell culture
Measurement device includes light-transmission tube and reading assembly.Preferably, light-transmission tube is managed for PCV, and PCV pipes are made of clear material, preferably
Transparent plastic.
The present embodiment relates to cell viability measurement device in a kind of cell culture.Cell viability in the cell culture
Measurement device includes light-transmission tube 100 and reading assembly 200.Preferably, light-transmission tube 100 is managed for PCV, and PCV pipes are by transparent material
It is made, preferably clear plastics.
Referring to shown in Fig. 1 and Fig. 2, the top of light-transmission tube 100 has the bottom lock of opening and light-transmission tube 100.Light-transmission tube
100 include the fixed part 110 positioned at top, the measurement portion 120 positioned at bottom and connection fixed part 110 and measurement portion 120
Transition part 130.Fixed part 110 is cylinder, and the internal diameter of fixed part 110 is 0.5cm-1cm.The internal diameter of measurement portion 120 is 400
μm -600 μm, be preferably 500 μm.Typical sizes in fixed part 110 are centimetre scale, and typical sizes are in fixed part 110
About 1 milliliter of order of magnitude.Measurement portion 120 can be designed as the capillary with about 500 μm of typical internal diameter, 120 allusion quotation of measurement portion
Type volume is about 5 μ L.
Referring to shown in Fig. 4 and Fig. 5, reading assembly 200 includes being protected from light housing 210 and being located at being protected from light consolidating in housing 210
Determine device 220, light-emitting device 230, image forming optics 240, imaging sensor 250, processing unit.
Fixing device 220 is used to fix light-transmission tube 100.Further, in one embodiment, fixing device 220 has
Card slot is read, card slot is read and is adapted to light-transmission tube 100.
Referring to shown in Fig. 4 and Fig. 5, light-emitting device 230 close to fixing device 220 for shining towards light-transmission tube 100,
Image forming optics 240 are opposite with fixing device 220.Preferably, light-emitting device 230 can be one or more light-emitting diodes
Pipe, light-emitting device 230 can equably irradiate the measurement portion 120 of light-transmission tube 100.In addition, light-emitting device 230 can be with
It is external laser.
For image forming optics 240 for being imaged to light-transmission tube 100, imaging sensor 250 is connected to image forming optics 240
For receiving the image-forming information of image forming optics 240.Preferably, imaging sensor 250 can be plane CCD camera,
Alternatively, line array sensor or scanning optical sensor can also be provided.
Processing unit is connected to imaging sensor 250 for obtaining image-forming information and being assessed according to preset rules.
Processing unit can carry out Digital data processing.Processing unit can be outer computer or be arranged in and be protected from light housing
Microprocessor in 210.The function of processing unit is assessed according to default rule, determines the height of cell cake, especially
It is to determine the height of each cell mass in sample container.
Further, referring to shown in Fig. 1 and Fig. 2, in one embodiment, the internal diameter of transition part 130 is by close to measurement portion
120 one end to one end close to fixed part 110 gradually increases.
Further, referring to shown in Fig. 1 and Fig. 2, in one embodiment, the outer diameter of measurement portion 120 is less than fixed part
110 outer diameter.
Preferably, referring to shown in Fig. 1 and Fig. 2, in one embodiment, the outer diameter of transition part 130 is by close to measurement portion 120
One end to one end close to fixed part 110 gradually increase namely 130 tapered gradual change of transition part, light-transmission tube 100 is in funnel
Shape.
Further, referring to shown in Fig. 1 and Fig. 2, in one embodiment, the outer wall of light-transmission tube 100 is connected at least one
To stabilizer 400, the position of two stabilizers 400 in each pair stabilizer 400 is opposite.The one side of each stabilizer 400 and survey
Amount portion 120 connects, and opposite side is connected with transition part 130.In the present embodiment, stabilizer 400 is a pair namely two.It is not difficult
Understand, the quantity of stabilizer 400 still can two pairs, three equities.Cell viability measurement device, passes through in above-mentioned cell culture
Stabilizer 400 is set to improve stability of the light-transmission tube 100 in movement, centrifugation.
Further, shown in Figure 1, in one embodiment, the lateral wall edge of two stabilizers 400 extends to
Concordant with the outer wall of fixed part 110, the bottom margins of two stabilizers 400 extends to concordant with the bottom of light-transmission tube 100.Stablize
The wing 400 preferably links into an integrated entity formula structure with the outer wall of the outer wall of measurement portion 120 and transition part 130, and stabilizer 400 is into flat
Plate, for given measurement portion 120, stabilizer 400 does not hinder to observe.
Further, shown in Figure 3, cell viability measurement device is also in one embodiment, in the cell culture
Including fixing bracket 300.There are multiple fixed card slots, fixed card slot is adapted to light-transmission tube 100 on fixing bracket 300.It is above-mentioned thin
Cell viability measurement device in born of the same parents' culture by setting fixing bracket 300, has multiple fixed card slots on fixing bracket 300,
Multiple fixed card slots can block for multiple light-transmission tubes 100 to be set, and the centrifuge that adaptation may be employed in fixing bracket 300 is centrifuged,
Multiple light-transmission tubes 100 can be inserted into centrifuge or taken out from centrifuge simultaneously, improve multiple light-transmission tubes 100 from
The uniformity of heart treatment conditions, while time power is also saved, operating efficiency is high.
Further, shown in Figure 3, fixing bracket 300 includes cover board 310, bottom plate 320 and isolation board 330, cover board
A multiple isolation boards 330 are connected between 310 and bottom plate 320, isolation board 330 divides the region between cover board 310 and bottom plate 320
Multiple above-mentioned fixed card slots are divided into, there are multiple passages on cover board 310, each passage is corresponding respectively to connect a fixing card
Slot, so that light-transmission tube 100 is inserted into.
Preferably, multiple sample retainers can also be provided to substitute fixing bracket 300, be especially suitable for accommodating above-mentioned solid
The sample retainer of fixed rack 300 is more preferably to provide manually or electrically driver, and automatic sampling and automatic sample is allowed to judge
Estimate.
Further, shown in Figure 4, in one embodiment, being protected from light housing 210, there is reading to be open, and be protected from light housing
Revolving door 211 is hinged on 210, for revolving door 211 for closing or opening reading opening, fixing device 220 is connected to rotation
On 211 surface inwardly of door.
Further, shown in Figure 5, in one embodiment, being protected from light housing 210 includes fixed plate 212, limiting plate
213 and it is protected from light clamshell.The quantity of limiting plate 213 is two.It is opposite that two limiting plates 213 are connected to fixed plate 212
Both sides.Two limiting plates 213 are parallel.One of limiting plate 213 is equipped with revolving door 211.Light-emitting device 230, image optics
Device 240 and imaging sensor 250 are each provided in fixed plate 212, are protected from light clamshell and are located at fixed plate 212 and limiting plate
On 213.
In above-mentioned cell culture cell viability measurement device in carrying out cell culture cell viability measure when, including
Following steps:
After cell and dyestuff are incubated time enough, by putting the cell suspending liquid after dyeing in light-transmission tube 100,
Cell suspending liquid is centrifuged in light-transmission tube 100, during centrifugation, 2500g is centrifuged about 1 minute and is particularly advantageous.Centrifugal force phase
It can cause the insufficient compression of cell cake to weaker and/or shorter centrifugation time.In the case where relative centrifugal force is big, centrifugation step
Cell cake meeting reflation is observed after rapid, this causes measurement inaccurate.
It is shown in Figure 3, the cell mass of compression in pipe is obtained after centrifugation, cell mass is subdivided into three of different height
Part, complete cell are deposited on since density is higher in the lower area 40 of capillary, and cell is not colored, because they
Cell membrane tolerance dyestuff infiltration.Dead cell and cell fragment deposit uppermost region 20 since density is higher, cell membrane
The infiltration of dyestuff is resistant to, therefore region is farthest dyed.The cell viability of intermediate region 30 is between 40 He of lower area
Between upper area 20, color gradient is formed in 30 region of intermediate region.By the way that the light-transmission tube 100 after centrifuging is placed in reading
It takes in the fixing device 220 of component 200, light-emitting device 230 shines towards light-transmission tube 100, and image forming optics 240 are to light-transmission tube
100 imagings, imaging sensor 250 receive the image-forming information of image forming optics 240, which is transferred to processing unit,
Processing unit is assessed according to default rule, determines the height of cell cake, is especially to determine each thin in sample container
The height of born of the same parents' agglomerate.Finally, the volume of cell mass is measured according to the height of cell cake, calculates volume shared by complete cell
Ratio.It is shown in Figure 7, the cell suspending liquid after dyeing is put to the cell mass of centrifugation acquisition compression, by measuring cell
The different coloured portions of agglomerate detect the cell proportion of different vigor, in general, in the case of typical cell culture, hair
Three differentiable parts of existing cell cake.In there are the uppermost region 20 of dead cell and cell fragment, it was found that uniformly
Utmostly dyeing.In the lower area 40 of complete cell, do not find to dye.In general, the extension between them
It is a transitional region namely intermediate region 30, it will show different shades in a manner of limited, be dyed from minimum to maximum.
Fig. 7 shows the dyeing graph of cell mass short transverse, dyes gradient.This dyeing measures in detail represents different damaging cells
Distribution.By the staining signals for measuring a large amount of cells so that the distribution is statistically effective.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.