CN108042620B - Method for extracting total alkaloids in aconitum sinomontanum nakai - Google Patents

Method for extracting total alkaloids in aconitum sinomontanum nakai Download PDF

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CN108042620B
CN108042620B CN201711322062.4A CN201711322062A CN108042620B CN 108042620 B CN108042620 B CN 108042620B CN 201711322062 A CN201711322062 A CN 201711322062A CN 108042620 B CN108042620 B CN 108042620B
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舒孝顺
张豪
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Changsha University of Science and Technology
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Abstract

The invention discloses a method for extracting total alkaloids in aconitum sinomontanum nakai, which comprises the following steps: 1) pretreatment of raw materials: drying and crushing the aconitum sinomontanum powder raw material, sieving a product, and taking fine powder as a raw material for extraction; 2) ultrasonic extraction: selecting an organic solvent as an extraction solvent, adding concentrated hydrochloric acid accounting for 0.2-0.5% of the total volume of the extraction solvent to prepare a mixed solvent, fully mixing the aconitum sinomontanum powder obtained in the step 1) with the mixed solvent, standing and soaking for 10-40min, carrying out ultrasonic reaction for 0.5-2.5h, and filtering out insoluble substances to obtain a filtrate; 3) repeating the extraction process of step 2) for 0-3 times, and mixing several times to obtain final filtrate. The invention has the advantages of simple and rapid operation, high extraction rate, high speed, high efficiency and energy conservation.

Description

Method for extracting total alkaloids in aconitum sinomontanum nakai
Technical Field
The invention relates to a method for extracting effective components from plants, in particular to a method for extracting total alkaloids from aconitum sinomontanum nakai.
Background
Aconitum sinomontanum is a plant of the genus Aconitum in the family Ranunculaceae, is produced in the west and middle parts of China, and the root of a traditional Chinese medicine is usually used as the medicine, so that the traditional Chinese medicine has the effects of promoting blood circulation to remove blood stasis, relieving pain and diminishing swelling and the like, and can be used for treating diseases such as cold joint pain due to wind-cold, rheumatism and the like. In recent years, the research on alkaloids in aconitum sinomontanum nakai is active all the time, each component is separated from total alkaloids, and various alkaloid medicaments which have the effects of easing pain and anaesthesia, good analgesic effect, no addiction or other pharmacological effects of promoting blood circulation to remove blood stasis, relieving pain and reducing swelling and the like are screened out through pharmacology, so that the aconitum sinomontanum nakai alkaloid compound has good market application and development prospect.
The main active ingredient of aconitum sinomontanum medicinal material is diterpene alkaloid, and 28 compounds have been isolated and identified from aconitum sinomontanum root, 27 of them are diterpene alkaloids, such as sinomenine A, sinomenine B, ranitidine, deacetylranitidine, sinomenine A, sinomenine B, sinomenine F, sinomenine H, sinomenine 1 apanine, rauretinine, enteromorphine, 8-O-acetylerucamine, sinomenine G, sinomenine D, sinomenine E, sinomenine C, sinomenine B, sinomenine D, sinomenine B, sinomenine B, sinomenine B, sinomenine B, letenine (lepenine), ranaconidine, and N- (chloromethyl) taspine, and the like.
At present, the extraction methods of alkaloid are many, and considering the simplicity and the production cost saving of the method, the prior art also discloses a novel extraction method: the ultrasonic extraction method can greatly improve the extraction efficiency, save the solvent and shorten the extraction time, so the method has great potential in the aspect of natural product extraction. The ultrasonic method utilizes the strong cavitation effect, mechanical vibration and other actions generated by ultrasonic radiation to damage cell membranes, so that the cells are broken, the alkaloid dissolution is facilitated, meanwhile, the substance molecular motion speed is increased by the oscillation effect of ultrasonic, the solute diffusion is facilitated, the penetrating power of a solvent is increased, the heat effect of the ultrasonic heats an extracting agent, the utilization rate of the extracting agent is improved, and the extraction of effective components is simply and efficiently promoted. Patent document CN102617468A discloses a method for ultrasound-assisted extraction of lappaconitine, but the extraction time of alkaloid by the current ultrasound method is long, the energy consumption is large, and the extraction rate is to be further improved.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the method for extracting the total alkaloids from aconitum sinomontanum nakai, which has the advantages of simple and quick operation, high extraction rate, high speed, high efficiency and energy conservation.
The technical scheme of the invention is as follows: a method for extracting total alkaloids in aconitum sinomontanum nakai comprises the following steps:
1) pretreatment of raw materials: drying and crushing the aconitum sinomontanum powder raw material, sieving a product, and taking fine powder as a raw material for extraction;
2) ultrasonic extraction: selecting an organic solvent as an extraction solvent, adding concentrated hydrochloric acid accounting for 0.2-0.5% of the total volume of the extraction solvent to prepare a mixed solvent, fully mixing the aconitum sinomontanum powder obtained in the step 1) with the mixed solvent, standing and soaking for 10-40min, carrying out ultrasonic reaction for 0.5-2.5h, and filtering out insoluble substances to obtain a filtrate;
3) repeating the extraction process of step 2) for 0-3 times: filtering after each extraction, repeating the operation of the step 2) on the filter residue for repeated extraction, and combining the extraction filtrates for several times to obtain the final filtrate.
The further technical scheme of the invention is as follows: the aconitum sinomontanum nakai powder and the mixed solvent are fully mixed according to the material-liquid ratio of 1:5-1:20g/mL in the step 2), and the mixed solvent formed by adding concentrated hydrochloric acid accounting for 0.2-0.4% of the volume of the mixed solvent and ethanol is used for forming salt in an acid solution, so that the aconitum sinomontanum nakai total alkaloid is more easily dissolved in an extraction solvent, and the aconitum sinomontanum nakai total alkaloid is more quickly dissolved in the mixed solvent and is more completely extracted.
Further, the ultrasonic reaction in the step 2) is carried out at 40-80 ℃, and the higher temperature can improve the solubility and the extraction speed of the aconitum sinomontanum total alkaloids in the adopted mixed solvent at the higher extraction temperature.
Further, the standing and soaking process in the step 2) is carried out at the temperature of 20-35 ℃, the soaking is carried out at the temperature, so that the mixed extraction solvent can fully permeate into the medicinal material particles, and after the solvent enters the medicinal material particles, the entering hydrochloric acid can salt the total alkaloids in the aconitum sinomontanum so as to be more easily dissolved in the mixed extraction solvent, thereby being beneficial to the extraction of the total alkaloids in the aconitum sinomontanum.
Further, the organic solvent in the step 2) is 40-95% of ethanol.
The traditional extraction method of total alkaloids in aconitum sinomontanum nakai has the defects of long extraction time, high energy consumption, low extraction rate and the like, and in order to improve the defects, an ultrasonic extraction method and a unique process are adopted to extract the total alkaloids in aconitum sinomontanum nakai.
Firstly, drying and crushing a aconitum sinomontanum medicinal material, sieving the medicinal material by a 200-mesh sieve, taking 200-mesh fine powder for extraction, adding 0.2 to 0.5 percent of hydrochloric acid into each extraction solvent before extraction because alkaloids such as lappaconitine are easily dissolved in an acid solution, accurately weighing 5.0g of aconitum sinomontanum powder into a beaker, adding methanol, ethanol, acetone, ethyl acetate and trichloromethane according to the material-liquid ratio of 1:10 respectively, soaking for 20 minutes at normal temperature (about 30 ℃), performing ultrasonic extraction for 1.0 hour at 50 ℃, filtering insoluble substances, taking 0.5mL of filtrate, fixing the volume to a 50mL bottle, and measuring the absorbance to obtain the optimal extraction solvent. Calculating the alkaloid content of the measured solution according to a regression equation C-52.574A-0.9863, and calculating the alkaloid extraction rate according to the following formula: the total alkaloid extraction rate (%) (% C × 50 × 10-4 (g)/the weight of the raw material (g) × 100% (wherein: C represents the calculated lappaconitine concentration), and the experimental results are shown in Table 1 and FIG. 1 below.
TABLE 1 selection of extraction solvents
Figure BDA0001504960220000041
As can be seen from table 1 and fig. 1: methanol has better extraction efficiency than ethanol, and the extraction rate of ethanol is 1.629%, but ethanol is selected as the extraction solvent because of high toxicity of methanol. The experiments of the following examples were carried out again on the basis of the determination of the extraction solvent.
Further, the ultrasonic reaction in the step 2) is carried out in an ultrasonic reactor, and the ultrasonic power is 170-190W.
Further, the sieve in the step 1) is a 150-250 mesh sieve, so that the extraction solvent can be rapidly diffused in the aconitum sinomontanum medicinal material particles.
The method for measuring the extraction rate of the total alkaloids in the filtrate obtained in the step 3) comprises the following steps: taking 0.5ml of filtrate, diluting to 50ml volumetric flask, measuring absorbance A of the filtrate by ultraviolet spectrophotometry, calculating concentration C of total alkaloid in the filtrate according to regression equation C-52.574A-0.9863, and extracting according to total alkaloid extraction rate C-C × 50 × 10-4The weight of the used raw materials is multiplied by 100 percent, and the extraction rate of the alkaloid in the filtrate obtained in the step 2) is calculated; the measurement of the absorbance A of the filtrate was carried out at a scanning speed of 2nm/s in the wavelength range of 200-600 nm.
Compared with the prior art, the invention has the following characteristics: according to the invention, firstly, an acidic reagent is added into an extraction solvent to adjust the pH value of the extraction solvent to be acidic, then, raw materials are added for soaking for a certain time, an acidic mixed extraction solvent can fully permeate into medicinal material particles, after the solvent enters the medicinal material particles, the entered hydrochloric acid enables the aconite total alkaloids to be salified and more easily dissolved in the mixed extraction solvent, so that the aconite total alkaloids are more quickly and thoroughly dissolved in the mixed solvent, the subsequent extraction of the aconite total alkaloids is facilitated, the ultrasonic extraction is carried out at 40-80 ℃ after the soaking, the solubility and the extraction speed of the aconite total alkaloids in the adopted mixed solvent can be improved at higher temperature, through the optimized combined processes, the total preparation time is shorter (not more than 4h), and the extraction rate of the finally obtained aconite total alkaloids is higher.
The detailed process of the present invention is further described below with reference to the accompanying drawings and the detailed description.
Drawings
FIG. 1 is a graph showing the effect of solvent species on the total alkaloid extraction rate;
FIG. 2 is a graph showing the effect of ultrasound time on the total alkaloid extraction rate in example 1 of the present invention;
FIG. 3 is a graph showing the effect of extraction temperature on the total alkaloid extraction rate in example 2 of the present invention;
FIG. 4 is a graph showing the effect of extractant concentration on total alkaloid extraction rate in example 3 of the present invention;
FIG. 5 is a comparison of the effect of the feed-liquid ratio on the total alkaloid extraction rate in example 4 of the present invention;
FIG. 6 is a standard curve diagram showing the relationship between total alkaloid concentration and absorbance in Aconitum sinomontanum nakai according to the embodiment of the present invention.
Detailed Description
Example 1:
accurately weighing 5.0g of the processed 150-mesh aconitum sinomontanum powder in a beaker, adding the selected optimal extraction solvent according to the material-liquid ratio of 1:10g/mL, adding 0.2% hydrochloric acid, soaking for 30min at 20 ℃, performing ultrasonic extraction for 0.5h, 1.0h, 1.5h, 2.0h and 2.5h at 50 ℃, filtering insoluble substances, taking 0.5mL of filtrate, fixing the volume to a 50mL volumetric flask, and measuring absorbance to obtain the optimal ultrasonic time. The extraction rate of the total alkaloid is calculated according to the formula C-52.574A-0.9863. The results of the experiment are shown in table 2 below and fig. 2:
TABLE 2 Effect of ultrasound time
Figure BDA0001504960220000051
Figure BDA0001504960220000061
As can be seen from table 2 and fig. 2: the total alkaloid extraction rate of 2.167% obtained by extracting for 2 hours is the highest, which is far more than 0.5 hour but slightly higher than the extraction rate of 1 hour and 1.5 hours. Along with the prolonging of the vibration time, the cell wall breaking is complete, so the extraction rate is improved. The optimal extraction time is selected to be 1 hour in consideration of saving time, fuel, manpower and the like.
Example 2:
accurately weighing 5.0g of the processed aconitum sinomontanum powder with 200 meshes in a beaker, adding a selected optimal extraction solvent according to the material-liquid ratio of 1:10g/mL, adding 0.3% hydrochloric acid, soaking for 20min at 30 ℃, performing ultrasonic extraction for 1.0h at 40 ℃, 50 ℃, 60 ℃, 70 ℃ and 80 ℃ respectively, filtering out insoluble substances, taking 0.5mL of filtrate, fixing the volume to a 50mL volumetric flask, and measuring the absorbance to obtain the optimal ultrasonic temperature. The extraction rate of the total alkaloid is calculated according to the formula C-52.574A-0.9863. The results of the experiment are shown in table 3 below and fig. 3:
TABLE 3 Effect of extraction temperature
Figure BDA0001504960220000062
As can be seen from table 3 and fig. 3: the total alkaloid extraction rate is highest at 70 deg.C, alkaloid dissolving capacity is enhanced at higher temperature, and 70 deg.C is selected as the best extraction time.
Example 3:
accurately weighing 5.0g of the processed 220-mesh aconitum sinomontanum powder in a beaker, respectively selecting ethanol solutions with the concentrations of 40%, 60%, 80%, 90% and 95% according to the optimal extraction conditions according to the material-liquid ratio of 1:10g/mL, adding 0.5% hydrochloric acid, carrying out pretreatment such as soaking for 35min at 25 ℃, respectively carrying out ultrasonic extraction for 1.0h, filtering out insoluble substances, taking 0.5mL of filtrate, fixing the volume to a 50mL volumetric flask, and measuring the absorbance to obtain the optimal extractant concentration. The extraction rate of the total alkaloid is calculated according to the formula C-52.574A-0.9863. The results are shown in table 4 below and fig. 4:
TABLE 4 Effect of extractant concentration
Figure BDA0001504960220000071
As can be seen from table 4 and fig. 4: the extraction rate of the obtained total alkaloids is gradually increased along with the increase of the ethanol concentration, and the total alkaloids reaches a peak value when the ethanol concentration is 80 percent; the extraction rate of total alkaloids is reduced along with the increase of ethanol concentration. Ethanol with proper concentration has promotion effect on extraction of alkaloid, and according to the principle of similarity and compatibility, when the ethanol concentration is 80%, the polarity of the ethanol is similar to the polarity of possible solute so as to achieve the maximum dissolution rate, so 80% is selected as the optimal extraction concentration.
Example 4:
accurately weighing 5.0g of the processed 180-mesh aconitum sinomontanum powder in a beaker, adding the optimal extraction solvent (namely 80% ethanol) namely 80% ethanol and 0.4% hydrochloric acid respectively according to the material-liquid ratio of 1:5g/mL, 1:10g/mL, 1:15g/mL and 1:20g/mL, soaking for 40min at 28 ℃, and the like for pretreatment, then carrying out ultrasonic extraction for 60min at 70 ℃, filtering out insoluble substances, taking 0.5mL of filtrate, fixing the volume to a 50mL volumetric flask, diluting the filtrate for researching the influence of the material-liquid ratio, and measuring the absorbance to obtain the optimal material-liquid ratio. And (4) calculating the total alkaloid extraction rate according to a formula C of 52.574A-0.9863. The results are shown in table 5 below and fig. 5:
TABLE 5 influence of the feed-to-liquid ratio
Figure BDA0001504960220000081
As can be seen from table 5 and fig. 5: in the early stage of extraction, the total alkaloid extraction rate is increased along with the increase of the material-liquid ratio, the penetration effect of an extraction solvent on medicinal materials is more sufficient, when the material-liquid ratio is 1:10g/mL, the total alkaloid extraction rate is highest, and because the material-liquid ratio is too large, the subsequent separation and purification work is difficult due to the overlarge volume of an extracting solution, so that 1:10g/mL is selected as the optimal material-liquid ratio.
Example 5:
accurately weighing 5.0g of the processed aconitum sinomontanum powder with 200 meshes in a beaker, adding 80% ethanol and 0.3% hydrochloric acid according to the material-liquid ratio of 1:10g/mL, carrying out pretreatment such as soaking for 10min at 35 ℃, carrying out ultrasonic extraction for 1.0h at 70 ℃, filtering out insoluble substances, taking 0.5mL of filtrate, fixing the volume to a 50mL volumetric flask, measuring absorbance, calculating the extraction rate of total alkaloids, and determining the optimal extraction frequency.
TABLE 6 determination of extraction times
Figure BDA0001504960220000082
As can be seen from Table 6, the total alkaloid extraction rate also increases with the increase of the extraction times, the highest extraction rate is reached when the extraction is carried out for the third time, and the total alkaloid extraction rate is reduced when the extraction is carried out for four times or five times, so that the continuous extraction times are selected to be three times in consideration of the production cost.
Orthogonal experiment:
ultrasonic-assisted extraction of aconitum sinomontanum total alkaloids is a complex process of interaction of various factors, in order to better discuss the influence of various factors on alkaloid extraction amount, an optimal process for extracting aconitum sinomontanum alkaloids by an ultrasonic-assisted method is determined, a related extraction model is established by an orthogonal test method, and the interaction of various influencing factors is investigated. According to the single-factor test result, the level range of the factors of the orthogonal test for ultrasonic-assisted extraction of the aconitum sinomontanum total alkaloids is determined as shown in the following table:
TABLE 7 factor horizon
Figure BDA0001504960220000091
TABLE 8 results of orthogonal experiments
Figure BDA0001504960220000092
As can be seen from tables 7 and 8, the factor influence was ranked primarily and secondarily according to the magnitude of the range R, and the larger R, the larger the influence of the factor on the experiment. The influence of the concentration of the C factor extractant on the extraction rate of the total alkaloids is the largest, and the influence of the extraction time, the material-liquid ratio and the extraction temperature on the extraction rate of the total alkaloids is the smallest. The optimal level is factor A, namely the extraction time is 1.0h, factor B, namely the extraction temperature is 60 ℃, factor C, the concentration of an extractant is 80% (0.2-0.5% hydrochloric acid is added), factor D, namely the material-liquid ratio is 1:15g/mL, the total alkaloids in aconitum sinomontanum nakai are soaked for 20 minutes before extraction and extracted for 3 times, and under the optimal condition, the extraction rate of the total alkaloids in aconitum sinomontanum nakai is 2.367 percent.
And (3) verification experiment:
carrying out verification experiments for three times according to the optimal process results obtained by the orthogonal experiment results, weighing 5.0g of the processed 200-mesh dried aconitum sinomontanum nakai powder, adding 80% ethanol solution (adding 0.2% -0.5% hydrochloric acid), extracting for 1.0h at 60 ℃, soaking for 20 minutes before extraction at a material-to-liquid ratio of 1:15g/mL, extracting for 3 times in each experiment, and under the condition, the extraction rate is averagely 2.409%.
As can be seen from the above examples, orthogonal experiments and verification experiments, the method of the invention has the advantages of simple process, high efficiency in extracting total alkaloids from aconitum sinomontanum nakai, laying a foundation for industrial production of aconitum sinomontanum nakai, short production period, low cost and high extraction rate, and can be used for large-scale production.
In the above embodiments, total alkaloids in aconitum sinomontanum nakai are used as reference substances, and the content of total alkaloids in an aconitum sinomontanum nakai extracting solution is measured by an ultraviolet spectrophotometry, so that simple, convenient and reliable detection is provided for tracking and monitoring in the total alkaloids extracting process, and an accurate and efficient method is provided for detecting the actual quality of a product; moreover, the above embodiments all comprise the following processes:
(1) preparation of Standard solutions
Accurately weighing 15mg of total alkaloids standard substance in radix Aconiti Sinomontani in 50mL volumetric flask, adding appropriate amount of methanol solution for dissolving, dissolving completely and metering volume to obtain stock solution (0.3 mg/mL). Precisely sucking 1mL of the standard substance, placing in a 10mL volumetric flask, adding methanol to constant volume to a certain scale, and shaking to obtain a standard solution of total alkaloids in Aconitum sinomontanum with a concentration of 30 μ g/mL.
(2) Selection of optimum wavelength
Taking 2mL of the standard solution, taking methanol as a blank control, scanning by using an ultraviolet-visible spectrophotometer at the wavelength of 200-600 by taking 2nm as an interval unit, wherein the experimental scanning result shows that the solution has a maximum absorption peak at the wavelength of 252-254 nm, and determining that the maximum absorption wavelength is 252 nm.
(3) Preparation of Standard Curve
Accurately weighing 50.5mg total alkaloid standard substance in radix Aconiti Sinomontani, placing in 50mL measuring flask, dissolving with methanol and diluting to constant volume to obtain stock solution, accurately sucking the reference substance solution, placing in 50mL measuring flask, and diluting to constant volume with methanol to desired volume. 0.5mL, 1.0 mL, 2.0 mL, 3.0 mL, 4.0mL of the solution was pipetted into a 10mL measuring flask, diluted to the mark with methanol, and the absorbance was measured at a wavelength of 252nm with methanol as a blank.
The absorbance was linearly regressed with concentration, the regression equation being: C-52.574A-0.9863, R2Control solutions were linear over a range of 4.37-39.86 μ g/mL, as shown in fig. 6, 0.9988.
The sample solution was left at room temperature for 0, 20, 24, and 41 hours in the dark, and the absorbance at a wavelength of 252nm was measured, which indicated that the stability was good.
(4) Determination of total alkaloid content in aconitum sinomontanum extract
Accurate aconitum sinomontanum powder of 5g, add right amount of extraction solvent, extract with the ultrasonic wave assisted extraction method, after filtering, absorb 0.5mL filtrate, add extraction solvent to fix the volume to the volumetric flask of 50mL, measure the absorbance at the most suitable wavelength, substitute the measured value into the regression equation of the standard curve, solve the concentration C (mug/mL) of total alkaloid in the extract, and calculate the extraction rate of total alkaloid in aconitum sinomontanum according to the following formula:
the total alkaloid extraction rate (%) (% C × 50 × 10-4 (g)/the weight of the used raw materials (g) × 100%, in the formula: c represents the calculated total alkaloid concentration.
Comparative example 1:
accurately weighing 5.0g of aconitum sinomontanum powder in a beaker, soaking 50mL of 50-90% ethanol as an extraction solvent at 35 ℃ for 30 minutes, then extracting for three times by heating reflux, combining three filtrates, concentrating to 5050mL, taking 0.5mL of the filtrate, fixing the volume to a 50mL volumetric flask, measuring absorbance, and calculating the extraction rate. The experiment is repeated for three times, the calculated average extraction rate is 1.143 percent, the yield is 51.71 percent lower than that of ultrasonic extraction (2.367 percent), the traditional heating reflux extraction time is long, 2 hours of extraction are needed for each time, the total extraction time is 6 hours, the temperature is increased to about 90 ℃ during extraction, the total alkaloids in aconitum sinomontanum nakai are damaged due to long-time heating, so the extraction rate is far lower than that of the ultrasonic extraction, the energy consumption is higher than that of the ultrasonic extraction (60 ℃), the extraction time is 1/2 of the traditional heating reflux extraction, the extraction temperature is low (60 ℃), and the microwave extraction has the obvious advantages of high efficiency, energy conservation, short period, low comprehensive cost, high extraction rate and the like.

Claims (3)

1. A method for extracting total alkaloids in aconitum sinomontanum nakai is characterized by comprising the following steps:
1) pretreatment of raw materials: drying and crushing the aconitum sinomontanum powder raw material, sieving the product with a sieve of 150 meshes and 250 meshes, and extracting the fine powder serving as the raw material;
2) ultrasonic extraction: selecting an organic solvent as an extraction solvent, adding concentrated hydrochloric acid accounting for 0.2-0.5% of the total volume of the extraction solvent to prepare a mixed solvent, fully mixing the aconitum sinomontanum powder obtained in the step 1) with the mixed solvent, standing and soaking for 10-40min, carrying out ultrasonic reaction for 0.5-2.5h, and filtering out insoluble substances to obtain a filtrate; wherein the organic solvent is 80% ethanol; fully mixing the aconitum sinomontanum powder and a mixed solvent according to the material-liquid ratio of 1:5-1:20 g/mL; standing and soaking at 20-35 deg.C;
3) repeating the extraction process of step 2) for 0-3 times: filtering after each extraction, repeating the operation of the step 2) on the filter residue for repeated extraction, and combining the extraction filtrates for several times to obtain the final filtrate.
2. The method for extracting total alkaloids in aconitum sinomontanum nakai according to claim 1, wherein the ultrasonic reaction in the step 2) is performed at 40-80 ℃.
3. The method for extracting total alkaloids in aconitum sinomontanum nakai according to claim 1, wherein the ultrasonic reaction in the step 2) is performed in an ultrasonic reaction instrument with ultrasonic power of 170-190W.
CN201711322062.4A 2017-12-12 2017-12-12 Method for extracting total alkaloids in aconitum sinomontanum nakai Expired - Fee Related CN108042620B (en)

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CN101967124A (en) * 2010-06-28 2011-02-09 南京泽朗医药科技有限公司 Separation and purification method for lappaconitine
CN102617468A (en) * 2012-03-07 2012-08-01 西北师范大学 Method for ultrasound-assisted extraction of lappaconitine
CN106565795A (en) * 2016-10-17 2017-04-19 大兴安岭林格贝寒带生物科技股份有限公司 Method for extracting alkaloid from bitter gourds

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CN102617468A (en) * 2012-03-07 2012-08-01 西北师范大学 Method for ultrasound-assisted extraction of lappaconitine
CN106565795A (en) * 2016-10-17 2017-04-19 大兴安岭林格贝寒带生物科技股份有限公司 Method for extracting alkaloid from bitter gourds

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