CN107998163A - A kind of refined substance of red ginseng and its preparation method and application - Google Patents
A kind of refined substance of red ginseng and its preparation method and application Download PDFInfo
- Publication number
- CN107998163A CN107998163A CN201711386842.5A CN201711386842A CN107998163A CN 107998163 A CN107998163 A CN 107998163A CN 201711386842 A CN201711386842 A CN 201711386842A CN 107998163 A CN107998163 A CN 107998163A
- Authority
- CN
- China
- Prior art keywords
- red ginseng
- preparation
- water
- liquid
- refined substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to the technical field of Chinese medical extract, more particularly to a kind of purification of tcm thing and preparation method and application.The refined substance carries through water using red ginseng as raw material, UF membrane, ion-exchange chromatography, is concentrated and dried to obtain red ginseng refined substance power-product.Red ginseng refined substance dry powder uniform color prepared by the present invention, solubility is good, purity is high, active constituent content is high and the effect of auxiliary treatment diabetes is preferable；Have the characteristics that preparation process is easy and effective in production process, be not required poisonous and hazardous organic solvent, cost low and discharged without " three wastes ".The product prepared using the method for the present invention can be widely applied in the industries such as medicine, health care, and have boundless market prospects, can produce huge economic benefit and good social benefit.
Description
Technical field
The present invention relates to a kind of preparation technique of traditional Chinese medicine, more particularly to a kind of extraction technology of preparing of effective component of chinese medicine and its
Using, and in particular to a kind of method for preparing red ginseng refined substance using UF membrane, ion-exchange chromatography and concentrate drying technology.
Background technology
Diabetes are a kind of chronic metabolic diseases of pancreas function lesion, caused by being lacked relatively or definitely with blood glucose, according to system
Meter, global diabetic's number are being continuously increased, it has also become endanger the third-largest killer of human health.Treatment diabetes at present
Most of medicine be to play a role by core of insulin, such as insulin type preparation or its sensitizer medicine, but these
Medicine is still undesirable to the patients with terminal effect of some diabetics particularly type ii diabetes, therefore finds and solve pancreas islet
The focus that the new drug of element resistance will compete as world diabetes field.
Chinese medicine has long medicinal history, is the treasure of Chinese traditional culture.Chinese medicine is a complex system, its
Clinical efficacy explanation wherein includes mass efficient material, while there is also some in Chinese medicine to bring the poisonous of security risk to have
Evil material.Therefore it is in one in traditional Chinese medicine research field important to extract its active ingredient to develop new and effective preparation technique of traditional Chinese medicine
Hold.Traditional Chinese medicine extraction is exactly to extract wherein effective ingredient to greatest extent using some new techniques so that the inherent matter of Chinese medicine preparation
Amount and clinical therapeutic efficacy improve, and the effect of Chinese medicine is played to greatest extent.Membrane separation technique has different points of separation
The function of son amount, the technology have with traditional separation method compared with separation process without phase-change, separative efficiency it is high, need not addition it is chemical
Reagent, mild condition, without component damage, flow is short the advantages that, with applied to separating effective Chinese medicine components.
Red ginseng is dry root of the cultivation product of Araliaceae ginseng Panax ginseng after steaming, its is sweet in flavor, micro-
Hardship, warm-natured, returns spleen, lung, the heart channel of Hang-Shaoyin, nourishing qi to stop, has and reinforces vital energy, and veins takes off admittedly, reinforces the spleen to benefit the lung, promote the production of body fluid, calm the nerves and other effects.
Modern pharmacology research shows that red ginseng has diabetes auxiliary effect well, and the traditional Chinese medical science says that diabetes are called diabetes, and ginseng
Recorded in Sheng Nong's herbal classic, the effect of promoting the production of body fluid to quench thirst, there is good effect to diabetes.Ginsenoside Rb2, can be significantly
Improve hyperlipidemia and promote the protein metabolism of liver and the hyperglycaemia of Inhibition test glycosuria animal.Therefore, exploitation red ginseng refines
The novel preparation process of thing has great importance.
The content of the invention
Present invention generally provides one kind red ginseng refined substance is prepared using UF membrane, ion-exchange chromatography and concentrate drying technology
Method, asked for solving in current red ginseng extraction process the technologies such as yield is low, process costs are high, preparation process stability difference
Topic.
To overcome the problems of the prior art, a kind of preparation method of red ginseng refined substance proposed by the present invention, including it is as follows
Step：
Water extracts：Red ginseng medicinal material is taken, adds water to carry out decoction 0.5-24h, leaching liquid, the dregs of a decoction, which add water, decocts about 0.5-
12h, leaching liquid, discards the dregs of a decoction；Merge liquid twice, up to aqueous extract；
Crude separation：Decoction liquor is subjected to UF membrane, used filter membrane specification is 0.22-0.65 μm or 500-1000kD,
Filter wash is carried out with purified water after having filtered, merges film permeate, obtains rough segmentation chaotropic；
Segment from：Rough segmentation chaotropic is subjected to ultrafiltration membrane classification separation, used ultrafiltration membrane specification is 1-100kD, filtering
Filter wash is carried out with purified water after complete, merges film permeate, obtains subdivision chaotropic；
It is refined：Subfractionation liquid is subjected to purifying decoloration by ion exchange column, column is collected and flows through liquid, obtain refined liquid,
It is concentrated and dried, up to red ginseng refined substance powder.
Wherein, it is purified water, distilled water or water for injection with water in water extraction step.
Wherein, use can be that hollow fiber form, rolled film, tubular membrane or plate and frame film carry out UF membrane.
Wherein, crude separation and segment from, filter wash number is 3-5 times.
Wherein, the structural formula of the aglucon of the ion-exchange chromatography resin for following molecular structural formula (i)~(iii) it
One, loading volume is 10-50 times of column volume：
Wherein, in purification step, concentrate drying technology used is freeze-drying or spray drying.
The present invention also proposes a kind of red ginseng refined substance according to prepared by the preparation method, and the turbidity of red ginseng refined substance is shallow
In or equal to 0.5 level number of turbidity standard turbidity standard, freeze-dried powder is highly soluble in water and solubility is not less than 0.5g/ml.
The present invention also proposes application of the red ginseng refined substance in the medicine for preparing auxiliary treatment diabetes.
In above application, the medicine is preparation, includes red ginseng refined substance or red ginseng refined substance and auxiliary material.
Compared with prior art, beneficial effects of the present invention：The present invention provides a kind of stable quality, and curative effect is reliable, biology
The red ginseng Chinese medicine preparation of the high auxiliary treatment diabetes of availability.The present invention provides the red ginseng Chinese medicine preparation of auxiliary treatment diabetes
Preparation method, this method is simple and feasible, meets oral drugs body absorption process, final obtained Chinese medicine preparation stability
Good, curative effect is reliably notable.The present invention is prepared red ginseng refined substance and is remarkably improved mesh using UF membrane and ion-exchange chromatography
Mark the purity of thing and the content of active ingredient, this method have saved medicinal material cost, using the teaching of the invention it is possible to provide be more advantageous to industrial production preparation
Condition, and ensure the stability and security of technique, red ginseng Chinese medicine is played its effect as far as possible.The features of the present invention is：
The method being combined using micro-filtration, ultrafiltration and chromatographic technique, can effectively remove lipid, pigment, heavy metal, agriculture it is residual etc. it is invalid into
Point, while avoid the use of organic reagent, reduce industrial production pollution；Active ingredient is more importantly remained, can be maximized
Improve the medical value of red ginseng medicinal material
Brief description of the drawings
Fig. 1 is red ginseng Aqueous extracts freeze-dried powder and red ginseng refined liquid freeze-dried powder and its schematic diagram of redissolution liquid.
Fig. 2 is Aqueous extracts and refined liquid HPLC analysis results.
Embodiment
Red ginseng refined substance proposed by the present invention and its preparation method and application is carried out below in conjunction with schematic diagram more detailed
Description, which show the preferred embodiment of the present invention, it should be appreciated that those skilled in the art can change described here
The present invention, and still realize the advantageous effects of the present invention.Therefore, description below is appreciated that for those skilled in the art
It is widely known, and be not intended as limitation of the present invention.
The creation point of the present invention is UF membrane, ion-exchange chromatography and concentrate drying technology being applied to carrying for Chinese medicine
In terms of taking purifying, significant technological progress is thus brought, solution Chinese medical extract poor appearance, color are deep and preserve easily precipitation etc.
Shortcoming.With reference to extractive technique, UF membrane filtering technique, chromatographic technique and concentration technique etc., development is set of, can industrialization height
Separation and preparation technology is imitated, that establishes different Chinese medicine effective efficiency components efficiently separates preparation process, and is removed in separation process
The poisonous and harmful substances such as pesticide residue, heavy metal, endotoxin, carry out the process exploitation from lab scale to pilot scale to industrialization, verify
With conversion, form the safe and efficient application for isolating and purifying the high-end preparation of Chinese medicine such as technology of preparing application, development characteristic formulations and push away
Wide research, the overall international competitiveness for improving Chinese medicine preparation.
Embodiment 1
Red ginseng 100g is weighed, adds 8 times of water 0.8L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 4 times of water
0.4L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
0.45 μm of microfiltration membranes is connected on film processing system, flow velocity 400rpm is set, circulates end flow velocity 1.5L/min, thoroughly
It is 18ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain red ginseng water and put forward rough segmentation chaotropic.
100kD ultrafiltration membranes are connected on film processing system, flow velocity 360rpm is set, circulate end flow velocity 1.2L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge permeate, obtain red ginseng water and carry subfractionation liquid.
By Q Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column company
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), red ginseng water carries subfractionation liquid and crosses Q columns by systems pumps, collects
Column flows through liquid, obtains refined liquid；Refined liquid is positioned over -40 DEG C of refrigerator freezing 5h, after be put into freeze dryer, vacuumize it is dry until
Moisture all removes, and collects freeze-dried powder, is red ginseng refined substance powder product.
Red ginseng water extract and refined substance are taken respectively, pass through the change of target peak shape after HPLC analyses before purification, HPLC conditions
It is as follows：(1) chromatographic column filler：Unitary C18 (5 μm, 4.6*250mm)；(2) mobile phase A：0.4% (V/V) phosphoric acid, flowing
Phase B：Acetonitrile；(3) flow velocity：1ml/min；(4) gradient condition：0-10min 5%B phases, 10-30min is from 5-30%B phases, 30-
60min is from 30-95%B phases, 60-70min 95%B phases.(5) column temperature：50℃；(6) Detection wavelength：205nm；(7) sample introduction body
Product：10μl.
From the appearance, red ginseng refined substance powder uniform color, any surface finish, quality are fine and smooth.Refined substance freeze-dried powder finished product
Dissolved rapidly after adding purified water, solution clarification is bright, and the dissolving of water extract freeze-dried powder is slow and muddy, the result is shown in Figure 1.HPLC points
The results show (as shown in Figure 2) is analysed, rear sample, which obtains main peak shape in collection of illustrative plates, before purification does not have significant change, according to both cumulative volumes
Calculated with integrated peak areas, red ginseng refined substance main component yield does not decline after purification.Result above fully indicates use
The method of the present invention prepares the advantage with high-purity in high yield of red ginseng refined substance.
Embodiment 2
Red ginseng 100g is weighed, adds 10 times of water 1L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 6 times of water
0.6L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
0.22 μm of microfiltration membranes is connected on film processing system, flow velocity 400rpm is set, circulates end flow velocity 1.5L/min, thoroughly
It is 16ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain red ginseng water and put forward rough segmentation chaotropic.
50kD ultrafiltration membranes are connected on film processing system, flow velocity 360rpm is set, circulate end flow velocity 1.2L/min, are passed through
End mean flow rate is 15ml/min, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash, often
Secondary 50ml, filter wash 5 times；Merge permeate, obtain red ginseng water and carry subfractionation liquid.
By DEAE Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), red ginseng water carries subfractionation liquid and crosses Q columns by systems pumps, receives
Clustered column flows through liquid, obtains refined liquid；Refined liquid is positioned over -40 DEG C of refrigerator freezing 5h, after be put into freeze dryer, vacuumize dry straight
All removed to moisture, collect freeze-dried powder, be red ginseng refined substance powder product.
Embodiment 3
Red ginseng 100g is weighed, adds 8 times of water 0.8L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 5 times of water
0.5L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
0.1 μm of microfiltration membranes is connected on film processing system, flow velocity 380rpm is set, circulates end flow velocity 1.4L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain red ginseng water and put forward rough segmentation chaotropic.
30kD ultrafiltration membranes are connected on film processing system, flow velocity 350rpm is set, circulate end flow velocity 1.2L/min, are passed through
End mean flow rate is 13ml/min, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash, often
Secondary 50ml, filter wash 5 times；Merge permeate, obtain red ginseng water and carry subfractionation liquid.
By WorkBeads 100TREN, (molecular structural formula of resin aglucon is) ion friendship
Change chromatographic column to be connected in tomographic system, design current velocity 10ml/min (250cm/h), red ginseng water carries subfractionation liquid and passes through systems pumps
Q columns are crossed, column is collected and flows through liquid, obtain refined liquid；Refined liquid is positioned over -40 DEG C of refrigerator freezing 5h, after be put into freeze dryer, take out true
Sky is dry up to moisture all removals, collects freeze-dried powder, is red ginseng refined substance powder product.
Embodiment 4
Red ginseng 100g is weighed, adds 12 times of water 1.2L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 6 times
Water 0.6L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
750kD ultrafiltration membranes are connected on film processing system, flow velocity 380rpm is set, circulate end flow velocity 1.3L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain red ginseng water and put forward rough segmentation chaotropic.
10kD ultrafiltration membranes are connected on film processing system, flow velocity 330rpm is set, circulate end flow velocity 1.1L/min, are passed through
End mean flow rate is 11ml/min, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash, often
Secondary 50ml, filter wash 5 times；Merge permeate, obtain red ginseng water and carry subfractionation liquid.
By Q Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column company
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), red ginseng water carries subfractionation liquid and crosses Q columns by systems pumps, collects
Column flows through liquid, obtains refined liquid；Refined liquid is crossed into spray dryer until moisture all removes, collection dried powder, is red ginseng
Refined substance powder product.
Embodiment 5
Red ginseng 100g is weighed, adds 15 times of water 1.5L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 6 times
Water 0.6L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
750kD ultrafiltration membranes are connected on film processing system, flow velocity 380rpm is set, circulate end flow velocity 1.3L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain red ginseng water and put forward rough segmentation chaotropic.
100kD ultrafiltration membranes are connected on film processing system, flow velocity 360rpm is set, circulate end flow velocity 1.2L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge permeate, obtain red ginseng water and carry subfractionation liquid.
By Q Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column company
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), red ginseng water carries subfractionation liquid and crosses Q columns by systems pumps, collects
Column flows through liquid, obtains refined liquid；Refined liquid is crossed into spray dryer until moisture all removes, collection dried powder, is red ginseng
Refined substance powder product.
The preferred embodiment of the present invention is above are only, does not play the role of any restrictions to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical solution and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
1. a kind of preparation method of red ginseng refined substance, it is characterised in that include the following steps：
Water extracts：Red ginseng medicinal material is taken, adds water to carry out decoction 0.5-24h, leaching liquid, the dregs of a decoction, which add water, decocts about 0.5-12h, filter
Taking liquid, discards the dregs of a decoction；Merge liquid twice, up to aqueous extract；
Crude separation：Decoction liquor is subjected to UF membrane, used filter membrane specification is 0.22-0.65 μm or 500-1000kD, filtering
Filter wash is carried out with purified water after complete, merges film permeate, obtains rough segmentation chaotropic；
Segment from：Rough segmentation chaotropic is subjected to ultrafiltration membrane classification separation, used ultrafiltration membrane specification is 1-100kD, after having filtered
Filter wash is carried out with purified water, merges film permeate, obtains subdivision chaotropic；
It is refined：Subfractionation liquid is subjected to purifying decoloration by ion exchange column, column is collected and flows through liquid, refined liquid is obtained, by it
It is concentrated and dried, up to red ginseng refined substance powder.
2. preparation method according to claim 1, it is characterised in that be purified water, distilled water with water in water extraction step
Or water for injection.
3. preparation method according to claim 1, it is characterised in that use can be hollow fiber form, rolled film, tubular type
Film or plate and frame film carry out UF membrane.
4. preparation method according to claim 1, it is characterised in that crude separation and segment from, filter wash number is 3-5
It is secondary.
5. preparation method according to claim 1, it is characterised in that the structure of the aglucon of the ion-exchange chromatography resin
Formula is one of following molecular structural formula (i)~(iii), and loading volume is 10-50 times of column volume：
6. preparation method according to claim 1, it is characterised in that in purification step, concentrate drying technology used is cold
Lyophilized dry or spray drying.
A kind of 7. red ginseng refined substance prepared by any one of them preparation method according to claim 1-6, it is characterised in that
The turbidity of red ginseng refined substance is shallower than or the turbidity standard equal to 0.5 level number of turbidity standard, and freeze-dried powder is highly soluble in water and molten
Xie Du is not less than 0.5g/ml.
8. application of the red ginseng refined substance in the medicine for preparing auxiliary treatment diabetes described in claim 7.
9. application according to claim 8, it is characterised in that the medicine is preparation, includes red ginseng refined substance or red ginseng
Refined substance and auxiliary material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711386842.5A CN107998163A (en) | 2017-12-20 | 2017-12-20 | A kind of refined substance of red ginseng and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711386842.5A CN107998163A (en) | 2017-12-20 | 2017-12-20 | A kind of refined substance of red ginseng and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107998163A true CN107998163A (en) | 2018-05-08 |
Family
ID=62060076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711386842.5A Pending CN107998163A (en) | 2017-12-20 | 2017-12-20 | A kind of refined substance of red ginseng and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107998163A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1977945A (en) * | 2005-11-30 | 2007-06-13 | 张勇 | Method for extracting active ingredient from red ginseng and lilyturf root |
| CN102462715A (en) * | 2010-11-19 | 2012-05-23 | 天津尖峰弗兰德医药科技发展有限公司 | Red ginseng extract pellet and preparation method thereof |
| CN104415070A (en) * | 2013-08-30 | 2015-03-18 | 河北以岭医药研究院有限公司 | Method for separating and purifying total ginsenoside from ginseng |
| CN106422787A (en) * | 2016-10-20 | 2017-02-22 | 合肥创想能源环境科技有限公司 | Membrane-method integrated technology for traditional Chinese medicine deep processing production |
-
2017
- 2017-12-20 CN CN201711386842.5A patent/CN107998163A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1977945A (en) * | 2005-11-30 | 2007-06-13 | 张勇 | Method for extracting active ingredient from red ginseng and lilyturf root |
| CN102462715A (en) * | 2010-11-19 | 2012-05-23 | 天津尖峰弗兰德医药科技发展有限公司 | Red ginseng extract pellet and preparation method thereof |
| CN104415070A (en) * | 2013-08-30 | 2015-03-18 | 河北以岭医药研究院有限公司 | Method for separating and purifying total ginsenoside from ginseng |
| CN106422787A (en) * | 2016-10-20 | 2017-02-22 | 合肥创想能源环境科技有限公司 | Membrane-method integrated technology for traditional Chinese medicine deep processing production |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102351956B (en) | Extraction method of morindea officinalis polysaccharide | |
| CN102351819A (en) | Extraction, purification and preparation method of high-purity salvianolic acid B | |
| CN104473919A (en) | Process for extracting caffeate from erigeron breviscapus | |
| CN100439319C (en) | Method for preparing salviol acid A | |
| CN101974095B (en) | Method for extracting and separating Chinese narcissus polysaccharides | |
| CN101306077B (en) | Chinese traditional injection preparation for treating cancer and its preparation method | |
| CN102228515B (en) | Separation and enrichment method of total flavones and total alkaloids of Lotus Plumule | |
| CN107245113A (en) | Corn silk polysaccharide extract with antitumaous effect and preparation method thereof | |
| CN101530449B (en) | Method of preparing honeysuckle extract by applying membrane filtration technology | |
| CN102228488A (en) | Preparation of Lysimachia capillipes Hemsl total saponin | |
| CN105497109A (en) | Process for producing milkvetch root total flavones through microbial fermentation technology | |
| CN102229627B (en) | Method for preparing stachyose by using water-extraction alcohol-precipitation of salvia miltiorrhiza | |
| CN104945532B (en) | The preparation method of Gynura divaricata polysaccharide | |
| CN108117575A (en) | A kind of extracting method of the element of the continuously extracting cordyceps from Cordyceps militaris and Cordyceps sinensis polysaccharide | |
| CN107998163A (en) | A kind of refined substance of red ginseng and its preparation method and application | |
| CN108042609A (en) | A kind of refined substance of radix scutellariae and its preparation method and application | |
| CN103251659A (en) | Preparation method of ginkgo leaf essence | |
| CN101390962B (en) | Preparation method of active components in root-bark tree peony by in-phase leaching and sub-item preparation | |
| CN102993288A (en) | Extraction method of pinellin | |
| CN106496346A (en) | A kind of isolation and purification method of white polysaccharide | |
| CN102078560B (en) | Compound cantharis total extract preparation and preparation method thereof | |
| CN104945530A (en) | Method for refining Korean pine pinecone polysaccharide from macroreticular resin | |
| CN105001286A (en) | Method for extracting tiliroside from Tibetan drug scindapsus aureus | |
| CN105294789A (en) | Preparation method of high-purity salidroside | |
| CN107982340A (en) | A kind of refined substance of Cortex Phellodendri and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180508 |
|
| RJ01 | Rejection of invention patent application after publication |