CN107991363A - A kind of BOD biology sensors and its preparation method and application - Google Patents
A kind of BOD biology sensors and its preparation method and application Download PDFInfo
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- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 19
- 238000009835 boiling Methods 0.000 claims abstract description 14
- 229920002301 cellulose acetate Polymers 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 8
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- G01N27/28—Electrolytic cell components
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- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1806—Biological oxygen demand [BOD] or chemical oxygen demand [COD]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
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Abstract
The present invention relates to a kind of BOD biology sensors and its preparation method and application, more particularly to a kind of BOD biology sensors and its preparation method and application.The preparation method prepares BOD biology sensors using the strain after screening, comprises the following steps：(1) cellulose acetate is fully mixed with boiling water, after being configured to folder film, cooling；(2) strain after screening, starch and polyvinyl alcohol are mixed, is applied to after mixing on the folder film, forms film；(3) film is aspirated, strain is closely attached on film inner wall；(4) adhesion agent is smeared on film, BOD biology sensors are made.BOD biology sensors stability prepared by the present invention is good, and the corresponding time is short, and preservation time length, is quick on the draw test water body BOD concentration.
Description
Technical field
The present invention relates to environmental monitoring, and in particular to a kind of biology sensor and its preparation method and application, especially
It is related to a kind of BOD biology sensors and its preparation method and application.
Background technology
With developing rapidly for environmental science, people pay attention to environmental problem further；It is dirty that environmental problem has been not only discharge
The human health problems caused by thing are contaminated, further relate to the protection and the ecological balance and maintenance mankind procreation, development of natural environment
Resource problem.
Wherein, it is even more emphasis of concern as the water body environment closely bound up with human survival.Biochemical oxygen demand (BOD)
(BOD) be water body environment monitoring in weigh organic pollutants content overall target.Traditional assay method of BOD has
BOD5/20 methods, in short-term inspection pressure type coulometry, day method, Ping Tai values method and Wa Bo Breathing Methods etc..At present, generally use both at home and abroad
BOD5 standard dilution determination methods, are specifically filled in water sample in closed dissolved oxygen bottle, and 20 DEG C of lucifuge culture 5d, measure respectively
Before and after culture in water sample dissolved oxygen mass concentration；Then every liter of sample is calculated with the difference of the mass concentration of dissolved oxygen before and after culture
The dissolved oxygen content (BOD5) of product consumption.This method is cumbersome, and time-consuming, and disturbing factor is more, to the technical requirements of operating personnel
It is higher and cannot reflect water quality condition in real time, it is impossible in time for environmental management and decision-making, scientific research, accidental pollution etc. provide science according to
According to can not meet the requirement that quickly measures in current environment monitoring.
BOD biology sensors (BODs) solve the problems, such as this to a certain extent.When BODs works, with the list of microorganism
One strain or mixed bacteria, when the generation of addition or the katabolism of BODs in water, cause micro- life as biosensor
The change or conversion of external source breathing pattern, couple the change of output current power in thing, sensor output under certain condition
The concentration of current value and BOD are in a linear relationship.
Microbial film is the core of BODs biology sensors, selected species, quantity and its immobilization side
Method determines the response characteristic and service life of BODs.Traditional microbial film is fixed using porous cellulose acetate film sandwich method
Strain, response time about 10min, service life is shorter, is only 17d or so.Fixed using polyethylene film and perforated membrane sandwich method
For strain measurement range up to 1-10mg/L, the response time is about 15min, and stability (i.e. service life) is 25d；With porous acetic acid
Cellulose membrane sandwich method is compared, though stability increases, the response time is longer.
The content of the invention
In order to solve the above technical problems, the present invention provides a kind of BOD biology sensors and its preparation method and application.This
Invention is prepared for that a kind of stability is good, the corresponding time is short, the preservation time using the specific proportioning of strain, starch and polyvinyl alcohol
Grow, to test sensitive BOD biology sensors of water body BOD concentration-responses and its preparation method and application.
For this purpose, present invention employs following technical scheme：
In a first aspect, the present invention provides a kind of preparation method of BOD biology sensors, using the strain after screening
The step of preparing BOD biology sensors, the preparation BOD biology sensors specifically include following steps：
(1) cellulose acetate is fully mixed with boiling water, after being configured to folder film, cooling；
(2) strain after screening, starch and polyvinyl alcohol are mixed, is applied on the folder film, is formed after mixing
Film；
(3) film is aspirated, strain is closely attached on film inner wall；
(4) adhesion agent is smeared on film, preservation after BOD biology sensors is made.
Preferably, the mass ratio of the strain in step (2) after screening, starch and polyvinyl alcohol is 1:(0.5-1.5):
(1.5-2.5), such as can be 1:0.5:1.5、1:0.6:1.6、1:0.7:1.8、1:1.4:2.3、1:0.8:2.3、1:1.5:
2.5、1:0.8:1.7 or 1:1:2.5, it is preferably 1:(0.8-1.2):(1.8-2.4), more preferably 1:1:2, and the model
All point values included in enclosing, it is no longer exhaustive herein due to the limitation of length.
Preferably, the biology sensor being prepared is subjected to preservation.
Preferably, the pH of the preservation is 7.0-7.8, for example, can be 7.0,7.1,7.2,7.3,7.4,7.5,7.6,
7.7 or 7.8, it is preferably 7.2-7.6, all point values included in 7.4-7.5, and the scope is more preferably, due to length
Limitation, it is no longer exhaustive herein.
Preferably, the mass concentration of the glycerine of the preservation is 7-23%, for example, can be 7%, 8%, 9%, 10%,
11%th, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22% or 23%, it is preferably 9-
All point values included in 20%, and the scope, it is no longer exhaustive herein due to the limitation of length；
Preferably, the temperature of the preservation is -15~-25 DEG C, for example, can be -15 DEG C, -16 DEG C, -17 DEG C, -18 DEG C, -
19 DEG C, -20 DEG C, -21 DEG C, -22 DEG C, -23 DEG C, -24 DEG C or -25 DEG C, are preferably what is included in -18~-20 DEG C, and the scope
All point values, it is no longer exhaustive herein due to the limitation of length.
Preferably, the mass ratio of step (1) cellulose acetate and boiling water is 1：(95-105), such as can be 1:
95、1:96、1:97、1:98、1:99、1:100、1:101、1:102、1:103、1:104 or 1:105, it is preferably 1：(98-100),
And all point values included in the scope, it is no longer exhaustive herein due to the limitation of length.
As optimal technical scheme, the preparation method of the BOD biology sensors specifically includes following steps：
(1) bacterial screening：Point of strain is carried out using four ride of pollutant gradient concentration spread plate combination tablet
From purifying, obtain pure culture and be placed in beef-protein medium after shake culture about 24h, concussion centrifugation, physiological saline
Elution, is made wet thallus, that is, the strain after screening；
(2) BOD biology sensors are prepared：By cellulose acetate and boiling water according to mass ratio 1：(95-105) is fully mixed,
After being configured to folder film, cool down at room temperature；By the strain after screening, starch and polyvinyl alcohol according to 1：(0.5-1.5)：(1.5-
2.5) mass ratio is applied on the folder film after mixing, forms film, the film is aspirated using suction pump,
Strain is closely attached on film inner wall, then smear adhesion agent on film and bonded each component, BOD bio-sensings are made
Device；
(3) preservation of BOD biology sensors：By manufactured BOD biology sensors in pH7.0-7.8, qualities of glycerin concentration
Preservation is carried out under conditions of being -15~-25 DEG C for 7-23% and temperature.
Second aspect, the present invention provide the BOD biology sensors that a kind of method as described in relation to the first aspect is prepared.
The third aspect, the present invention provide a kind of BOD biology sensors as described in second aspect in water body BOD context of detection
Application.
Preferably, the application comprises the following steps：
(1) water body of different BOD5 concentration is respectively configured；
(2) the dissolved oxygen decreasing value of water body under the difference BOD5 concentration is measured respectively (i.e. with the BOD biology sensors
DO decreasing value)；
(3) standard curve of the BOD5 concentration with dissolved oxygen decreasing value of water body is made；
(4) the BOD biology sensors, the dissolved oxygen of water body to be measured are added after the dissolved oxygen value of water body to be measured is stablized
After dropping to another stationary value, the difference of both sides dissolved oxygen value is tried to achieve, water body to be measured is drawn by step (3) described standard curve
BOD values.
Using the BOD biology sensors of the present invention as biosensor, add in water body to be measured；When water body BOD concentration
When changing, the change or conversion of microorganism (i.e. BOD biology sensors) interior external source breathing pattern can be caused, in sample to be tested
Dissolved oxygen content (DO values) changes, and causes the change for coupling output current power for flowing into oxygen electrode, therefore, in certain bar
The concentration of BOD biology sensors dissolved oxygen content and BOD are in a linear relationship under part, so as to draw water body to be measured according to standard curve
The concentration of BOD.
Preferably, the scope of step (1) the water body BOD5 concentration is 15-100mg/L, and water body BOD5 concentration differs 5-
10mg/L；For instance, it may be preferable to concentration gradient be 15mg/L, 20mg/L, 25mg/L, 30mg/L, 35mg/L, 40mg/L, 45mg/
L, 50mg/L, 55mg/L, 60mg/L, 65mg/L, 70mg/L, 75mg/L, 80mg/L, 85mg/L, 90mg/L, 95mg/L and
100mg/L。
Preferably, the actual temp of step (2) the measure dissolved oxygen decreasing value is 30-35 DEG C, such as can be 30 DEG C,
31 DEG C, 32 DEG C, 33 DEG C, 34 DEG C or 35 DEG C；
Preferably, the specific aeration intensity of step (2) the measure dissolved oxygen decreasing value is 20-25h/L, such as can be
20h/L, 21h/L, 22h/L, 23h/L, 24h/L or 25h/L.
Compared with prior art, the present invention at least has the advantages that：
(1) the BOD biology sensors stability that prepared by method of the invention is good, and the response time is short, and soak time is short, solves
In the prior art the thalline of biology sensor be easy to run off with the response time it is long the problem of, also extend the use of biology sensor
Service life；
(2) through the present invention method preservation BOD biology sensors can continuously preservation to 6 months mycoderm stability without notable
Difference, reduces the cost and step of test indirectly；
(3) BOD biology sensors of the invention are sensitive to the reacting condition of water body BOD contents, can reach in environmental monitoring
The requirement quickly tested, can be widely used for the detection of water body BOD contents, and replicability is stronger.
Brief description of the drawings
Fig. 1 is the standard curve of BOD5 concentration and dissolved oxygen decreasing value.
Embodiment
For ease of understanding the present invention, it is as follows that the present invention enumerates embodiment.Those skilled in the art are it will be clearly understood that the implementation
Example is only to aid in understanding the present invention, is not construed as the concrete restriction to the present invention.
1 bacteria selection of embodiment
The bacterial screening of the present embodiment is hung oneself the active bed mud of long-term organic pollution domestication, utilizes pollutant gradient concentration
Four ride of spread plate combination tablet is isolated and purified, and is obtained pure culture and is named as MG0527.MG0527 is placed in ox
Shake culture 24h (37 DEG C, 140r/min) in meat extract peptone culture medium, then shakes centrifugation 8mim, physiology with 4000r/min
Salt water abandons supernatant for 4 times and wet thallus (strain after screening) is made, and adds 4 DEG C of PBS buffer and saves backup.
After carrying out Gram's staining to the strain after screening, microscopy shows that the strain is Gram-positive bacillus, and has bud
Spore, illustrates that the strain has stronger resistance；In addition, the strain has preferable resistance to low temperature, in -20 DEG C of continuous preservations
After 7 days, activate again, strain performance is stablized, and is mainly manifested in stable propagation performance, colonial morphology, resistance and Starch Hydrolysis
The stabilization of performance.Then, using the general 16S rRNA gene primers of bacterium to MG0527 carry out PCR amplification, after sequencing
BLAST phylogenetic tree constructions are carried out on NCBI, determines that the strain reaches 99.9% with certain bacillus similarity, can confirm institute
It is certain bacillus that NCBI is shown to survey strain.
The preparation of 2 BOD biology sensors of embodiment
(1) by cellulose acetate and boiling water according to mass ratio 1:98 fully mix, and after being configured to folder film, cool down at room temperature；
(2) by the strain after screening, starch and polyvinyl alcohol according to 1:0.5:2.5 mass ratio is applied to after mixing
On the folder film, film is formed；
(3) film is aspirated, strain is closely attached on film inner wall；
(4) and then on film adhesion agent is smeared, BOD biology sensors are made.
The preparation of 3 BOD biology sensors of embodiment
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 1:1.5:Outside 1.5, remaining and 2 step of embodiment
It is rapid identical.
The preparation of 4 BOD biology sensors of embodiment
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 1:1:Outside 2, remaining and 2 step phase of embodiment
Together.
The preparation of 5 BOD biology sensors of embodiment
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 1:1:Outside 1.5, remaining and 2 step of embodiment
It is identical.
The preparation of 6 BOD biology sensors of embodiment
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 1:1:Outside 2.5, remaining and 2 step of embodiment
It is identical.
The preparation of 1 BOD biology sensors of comparative example
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 1:1:Outside 5, remaining and 2 step phase of embodiment
Together.
The preparation of 2 BOD biology sensors of comparative example
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 1:0.1：1, remaining is identical with 2 step of embodiment.
The preparation of 3 BOD biology sensors of comparative example
Except the mass ratio of the strain after screening, starch and polyvinyl alcohol is 0.1:1:Outside 1.5, remaining and 2 step of embodiment
It is rapid identical.
The preparation of 7 BOD biology sensors of embodiment
(1) by cellulose acetate and boiling water according to mass ratio 1:99 fully mix, and after being configured to folder film, cool down at room temperature；
(2) by the strain after screening, starch and polyvinyl alcohol according to 1:1:2 mass ratio is applied to described after mixing
Press from both sides on film, form film；
(3) film is aspirated, strain is closely attached on film inner wall；
(4) and then on film adhesion agent is smeared, BOD biology sensors are made.
The preparation of 8 BOD biology sensors of embodiment
(1) by cellulose acetate and boiling water according to mass ratio 1:100 fully mix, and after being configured to folder film, cool down at room temperature；
(2) by the strain after screening, starch and polyvinyl alcohol according to 1:1:2 mass ratio is applied to described after mixing
Press from both sides on film, form film；
(3) film is aspirated, strain is closely attached on film inner wall；
(4) and then on film adhesion agent is smeared, BOD biology sensors are made.
The preparation of 4 BOD biology sensors of comparative example
Except cellulose acetate and boiling water are according to mass ratio 1：Outside 150, remaining is identical with 2 step of embodiment.
The preparation of 5 BOD biology sensors of comparative example
Except cellulose acetate and boiling water are according to mass ratio 1：Outside 50, remaining is identical with 2 step of embodiment.
The preparation of 6 BOD biology sensors of comparative example
Except cellulose acetate and boiling water are according to mass ratio 1：Outside 70, remaining is identical with 2 step of embodiment.
9 performance test of embodiment
The BOD biology sensors prepared using CH-1 types BOD intelligent biologicals detector to embodiment 2-8 and comparative example 1-6
It is tested for the property：
Each 2-4h is carried out to standard specimen, the measure of continuous 30 times, tests maximum difference deviation and first response time, as a result
As shown in table 1.
Maximum relative deviation=[(BOD values-average value of test object) ÷ average values] × 100%
1 test result of table counts
As it can be seen from table 1 embodiment 2-8 test datas, in the range of standard specimen guarantee value, maximum difference deviation is about
5-6%, the relative standard deviation values allowed less than standard liquid.It is the first response time about 8-11min, average in 9min or so.And comparative example
The maximum difference deviation of 1-6 is 15-20%, and the first response time is 12-15min, illustrates embodiment 2-8 and comparative example 1-6 phases
Than the BOD biology sensors of preparation have more preferable stability, and shorter response time, and shorter the time required to activation, say
The mass ratio of strain, starch and polyvinyl alcohol after the screening that the bright present invention selects, and the quality of cellulose acetate and boiling water
Than having conclusive influence for the performance of BOD biology sensors, effect is more notable in the range of the present invention is set.
Embodiment 10
By BOD biology sensors made of embodiment 2 in pH7.0, qualities of glycerin concentration is 7% and temperature is -25 DEG C
Under the conditions of carry out preservation.
Embodiment 11
By BOD biology sensors made of embodiment 2 in pH7.8, qualities of glycerin concentration is 23% and temperature is -15 DEG C
Under the conditions of carry out preservation.
Embodiment 12
By BOD biology sensors made of embodiment 2 in pH7.4, qualities of glycerin concentration is 15% and temperature is -20 DEG C
Under the conditions of carry out preservation.
Embodiment 13
By BOD biology sensors made of embodiment 2 in pH7.5, qualities of glycerin concentration is 10% and temperature is -25 DEG C
Under the conditions of carry out preservation.
Embodiment 14
By BOD biology sensors made of embodiment 2 in pH7.2, qualities of glycerin concentration is 17% and temperature is -20 DEG C
Under the conditions of carry out preservation.
Comparative example 7
In addition to pH is 5.5, remaining operation and 10 all same of embodiment.
Comparative example 8
In addition to pH is 9.5, remaining operation and 10 all same of embodiment.
Comparative example 9
In addition to glycerol concentration is 3%, remaining operation and 10 all same of embodiment.
Comparative example 10
In addition to glycerol concentration is 30%, remaining operation and 10 all same of embodiment.
Comparative example 11
In addition to preservation temperature is -5 DEG C, remaining operation and 10 all same of embodiment.
Comparative example 12
In addition to preservation temperature is -55 DEG C, remaining operation and 10 all same of embodiment.
15 performance test of embodiment
The BOD biology sensors of preservation in embodiment 10-14 and comparative example 7-12 are carried out stablizing mycoderm stability test
(the BOD biology sensors for testing preservation 1 month, 2 months, 4 months, 6 months, 8 months and 10 months respectively), as a result such as table 2
It is shown.From table 2 it can be seen that to activate the response time again bright for the mycoderm of the BOD biology sensors through preservation in embodiment 10-14
It is aobvious to be better than comparative example 7-11, remain basically stable with comparative example 12；But since the preservation temperature of comparative example 12 is too low, it is necessary to which special set
It is standby, therefore cost is higher.
In conclusion the storage conditions of the BOD biology sensors of the present invention can be up to the continuous preservation of BOD biology sensors
6 months, and mycoderm stability is without significant difference.
2 mycoderm of table activates the response time (min) again
| Test object | 1 month | 2 months | 4 months | 6 months | 8 months | 10 months |
| Embodiment 10 | 11 | 11 | 11 | 12 | 15 | 15 |
| Embodiment 11 | 13 | 14 | 13 | 15 | 15 | 15 |
| Embodiment 12 | 12 | 12 | 13 | 14 | 14 | 16 |
| Embodiment 13 | 10 | 11 | 11 | 12 | 13 | 14 |
| Embodiment 14 | 10 | 10 | 11 | 12 | 12 | 14 |
| Comparative example 7 | 23 | 27 | — | — | — | — |
| Comparative example 8 | 22 | 25 | — | — | — | — |
| Comparative example 9 | 27 | 29 | — | — | — | — |
| Comparative example 10 | 25 | 25 | 27 | — | — | — |
| Comparative example 11 | 24 | 25 | — | — | — | — |
| Comparative example 12 | 12 | 11 | 13 | 13 | 13 | 13 |
The reappearance measure of 16 water body BOD content detections of embodiment
Retest is carried out to the sample that BOD5 is 50mg/L using BOD biology sensors prepared by embodiment 2, is tied
Fruit is shown in Table 3, and specific method is as follows：
(1) water body that different BOD5 concentration are respectively configured (takes 15mg/L, 20mg/L, 25mg/L, 30mg/L, 35mg/ respectively
L、40mg/L、45mg/L、50mg/L、55mg/L、60mg/L、65mg/L、70mg/L、75mg/L、80mg/L、85mg/L、90mg/
L, 95mg/L and 100mg/L)；
(2) dissolved oxygen decreasing value (the i.e. DO drops of the water body under various concentrations are measured respectively with the BOD biology sensors
Low value)；
(3) standard curve of BOD5 concentration and dissolved oxygen decreasing value is made；
Standard curve as shown in Figure 1, it can be seen from the figure that DO decreasing value and BOD5 concentration have preferable linear relationship,
Linearly dependent coefficient reaches 0.98, disclosure satisfy that the requirement that water body BOD values to be measured quickly measure；
(4) the BOD biology sensors are added after the dissolved oxygen value of sample to be tested is stablized, treats the dissolved oxygen of sample to be tested
After dropping to another stationary value, the difference of both sides dissolved oxygen value is tried to achieve, water body to be measured is drawn by step (3) described standard curve
BOD values.
The results show that it is 0 that the average value of 5 measurement results, which is 0.3mg/L maximum relative deviations, meet traditional standard on the five
For the requirement of maximum deviation in dilution method.Illustrate that the reappearance of the BOD biology sensors of the present invention is stablized.
The reappearance of 3 BOD of table measure
Applicant states that the present invention illustrates the detailed process equipment of the present invention and technological process by above-described embodiment,
But the invention is not limited in above-mentioned detailed process equipment and technological process, that is, it is above-mentioned detailed not mean that the present invention has to rely on
Process equipment and technological process could be implemented.Person of ordinary skill in the field it will be clearly understood that any improvement in the present invention,
The addition of equivalence replacement and auxiliary element to each raw material of product of the present invention, selection of concrete mode etc., all fall within the present invention's
Within protection domain and the open scope.
Claims (10)
1. a kind of preparation method of BOD biology sensors, it is characterised in that BOD bio-sensings are prepared using the strain after screening
Device, comprises the following steps：
(1) cellulose acetate is fully mixed with boiling water, after being configured to folder film, cooling；
(2) strain after screening, starch and polyvinyl alcohol are mixed, is applied to after mixing on the folder film, forms film；
(3) film is aspirated, strain is closely attached on film inner wall；
(4) adhesion agent is smeared on film, BOD biology sensors are made.
2. the preparation method of BOD biology sensors as claimed in claim 1, it is characterised in that the bacterium in step (2) after screening
The mass ratio of kind, starch and polyvinyl alcohol is 1:(0.5-1.5):(1.5-2.5), is preferably 1:(0.8-1.2):(1.8-2.4),
More preferably 1:1:2.
3. the preparation method of BOD biology sensors as claimed in claim 1 or 2, it is characterised in that the biology that will be prepared
Sensor carries out preservation；
Preferably, the pH of the preservation is 7.0-7.8, is preferably 7.2-7.6, more preferably 7.4-7.5；
Preferably, the mass concentration of the glycerine of the preservation is 7-23%, is preferably 9-20%；
Preferably, the temperature of the preservation is -15~-25 DEG C, is preferably -18~-20 DEG C.
4. the preparation method of the BOD biology sensors as described in one of claim 1-3, it is characterised in that step (1) described vinegar
The mass ratio of acid cellulose and boiling water is 1:(95-105), is preferably 1:(98-100).
5. the preparation method of the BOD biology sensors as described in one of claim 1-4, it is characterised in that comprise the following steps：
(1) bacterial screening：The separation that strain is carried out using four ride of pollutant gradient concentration spread plate combination tablet is pure
Change, obtain pure culture and be placed in beef-protein medium after shake culture about 24h, concussion centrifugation, physiological saline elution,
Wet thallus, that is, the strain after screening is made；
(2) BOD biology sensors are prepared：By cellulose acetate and boiling water according to mass ratio 1:(95-105) is fully mixed, and is prepared
Into after folder film, cool down at room temperature；By the strain after screening, starch and polyvinyl alcohol according to 1:(0.5-1.5):(1.5-2.5's)
Mass ratio is applied on the folder film after mixing, forms film, the film is aspirated, strain is closely attached to thin
On film inner wall, then adhesion agent is smeared on film, BOD biology sensors are made；
(3) preservation of BOD biology sensors：In pH7.0-7.8, qualities of glycerin concentration it is 7- by manufactured BOD biology sensors
23% and temperature be -15~-25 DEG C under conditions of carry out preservation.
A kind of 6. BOD biology sensors that method as described in one of claim 1-5 is prepared.
7. BOD biology sensors as claimed in claim 6 are in the application of water body BOD context of detection.
8. application as claimed in claim 7, it is characterised in that comprise the following steps：
According to the linear relationship of the dissolved oxygen decreasing value that BOD biology sensors oxygen electrode exports and water body BOD concentration to be measured, draw
The BOD concentration of water body to be measured.
9. application as claimed in claim 7 or 8, it is characterised in that comprise the following steps：
(1) water body of different BOD5 concentration is respectively configured；
(2) the dissolved oxygen decreasing value of water body under the difference BOD5 concentration is measured respectively with the BOD biology sensors；
(3) standard curve of water body BOD5 concentration and dissolved oxygen decreasing value is made；
(4) the BOD biology sensors are added after the dissolved oxygen value of water body to be measured is stablized, the dissolved oxygen of water body to be measured declines
To after another stationary value, the difference of both sides dissolved oxygen value is tried to achieve, the BOD of water body to be measured is drawn by step (3) described standard curve
Value.
10. application as claimed in claim 9, it is characterised in that the scope of step (1) the water body BOD5 concentration is 15-
100mg/L, and water body BOD5 concentration difference 5-10mg/L；
Preferably, the actual temp of step (2) the measure dissolved oxygen decreasing value is 30-35 DEG C；
Preferably, the specific aeration intensity of step (2) the measure dissolved oxygen decreasing value is 20-25h/L.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0632620B2 (en) * | 1984-01-14 | 1994-05-02 | 日新電機株式会社 | Microbial membrane and method for producing the same |
| US20040168980A1 (en) * | 2002-01-04 | 2004-09-02 | Musale Deepak A. | Combination polymer treatment for flux enhancement in MBR |
| CN101639472A (en) * | 2009-08-31 | 2010-02-03 | 清华大学 | Reactor type BOD rapid measuring method and measuring instrument |
| CN102608181A (en) * | 2012-04-10 | 2012-07-25 | 中国科学院长春应用化学研究所 | Method for detecting biochemical oxygen demand |
| CN106830294A (en) * | 2017-01-23 | 2017-06-13 | 和县伊迈炭业有限责任公司 | A kind of method that utilization mushroom bran prepares bio-filter stuffing pore creating material |
-
2017
- 2017-11-24 CN CN201711190156.0A patent/CN107991363A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0632620B2 (en) * | 1984-01-14 | 1994-05-02 | 日新電機株式会社 | Microbial membrane and method for producing the same |
| US20040168980A1 (en) * | 2002-01-04 | 2004-09-02 | Musale Deepak A. | Combination polymer treatment for flux enhancement in MBR |
| CN101639472A (en) * | 2009-08-31 | 2010-02-03 | 清华大学 | Reactor type BOD rapid measuring method and measuring instrument |
| CN102608181A (en) * | 2012-04-10 | 2012-07-25 | 中国科学院长春应用化学研究所 | Method for detecting biochemical oxygen demand |
| CN106830294A (en) * | 2017-01-23 | 2017-06-13 | 和县伊迈炭业有限责任公司 | A kind of method that utilization mushroom bran prepares bio-filter stuffing pore creating material |
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