CN107982573A - A kind of preparation method of the internal styptic sponge of absorbable and degradable - Google Patents

A kind of preparation method of the internal styptic sponge of absorbable and degradable Download PDF

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Publication number
CN107982573A
CN107982573A CN201711311079.XA CN201711311079A CN107982573A CN 107982573 A CN107982573 A CN 107982573A CN 201711311079 A CN201711311079 A CN 201711311079A CN 107982573 A CN107982573 A CN 107982573A
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collagen
degradable
solution
powder
styptic sponge
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Inventor
张正男
付迎坤
常聪
王娜
张娟
张鹏
王喜卫
可永梅
于淑杰
徐守亚
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Henan Yadu Industrial Co Ltd
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Henan Yadu Industrial Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/001—Use of materials characterised by their function or physical properties
    • A61L24/0015—Medicaments; Biocides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/001—Use of materials characterised by their function or physical properties
    • A61L24/0042—Materials resorbable by the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
    • A61L24/10—Polypeptides; Proteins
    • A61L24/102—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
    • A61L24/10—Polypeptides; Proteins
    • A61L24/104—Gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents

Abstract

A kind of internal styptic sponge of absorbable and degradable, including cellular skeleton, cellular skeleton is collagenic protein skeleton, biological hemostatic granular powder is filled with cellular skeleton, cellular skeleton is coated with degradable membrane, degradable membrane is mixed using seaweed and gelatin, and degradable membrane is coated with latex film.Relative to the prior art; the present invention technique effect be; the latex film of outer layer is protective layer; after latex film is torn during use by styptic sponge patch side at wound; after the degradable membrane of collagen outer wrapping is degraded, the hemostasis granules powder of filling is acted at wound at the same time in collagen and collagen scaffold, and haemostatic effect is more preferable; anthemorrhagic speed is fast, improves the healing ability of wound.

Description

A kind of preparation method of the internal styptic sponge of absorbable and degradable
Technical field
The present invention relates to field of medical products, and in particular to a kind of preparation side of the internal styptic sponge of absorbable and degradable Method.
Background technology
During which surgical operation and wound all can have a large amount of loss of blood, it is necessary in time using hemostasis formed with the blood surface of a wound Means.The use of the Absorbable hemostatic material with biocompatibility is a kind of common hemostasis means for surface of a wound hemostasis, outside There are active demand in shell surgical hemostasis, wound, first aid, family when saving oneself.
The content of the invention
For above-mentioned technical problem, the object of the present invention is to provide a kind of preparation of the internal styptic sponge of absorbable and degradable Method, styptic sponge can directly act on and the surface of a wound, rapidly, effectively stops blooding.
Technical scheme is specially：
A kind of internal styptic sponge of absorbable and degradable, including cellular skeleton, cellular skeleton are collagenic protein skeleton, bee Be filled with biological hemostatic granular powder in nest shape skeleton, cellular skeleton is coated with degradable membrane, degradable membrane using seaweed with Gelatin is mixed, and degradable membrane is coated with latex film.
The biological hemostatic granular powder includes 5~10 parts of Carbazochrome Sodium Sulfonate, 8~15 parts of Radix Notoginseng powder, 5~10 parts of Folium Artemisiae Argyi powder, celestial being 15~30 parts of crane grass, 1~3 part of Diaceturate Ethylenediamine, 12~15 parts of qinghaosu, 90~110 parts of physiological saline.
The preparation method of the biological hemostatic granular powder includes,
Step 1：According to mass fraction proportioning weigh Carbazochrome Sodium Sulfonate, Radix Notoginseng powder, Folium Artemisiae Argyi powder, hairyvein agrimony, Diaceturate Ethylenediamine, Qinghaosu, physiological saline；
Step 2：Hairyvein agrimony is put into extractor, adds physiological saline, after being heated to boiling 40min, with a diameter of 0.18 μm Filtering with microporous membrane obtains extracting solution, degerming spare；
Step 3：Carbazochrome Sodium Sulfonate, Radix Notoginseng powder, Folium Artemisiae Argyi powder, Diaceturate Ethylenediamine, qinghaosu add extracting solution, are stirred with glass bar Metal is poured into after mixing uniformly and freezes disk, is covered with preservative film, is put into -90 DEG C of refrigerators and is carried out precooling 24h, is allowed to fully become Solid, after being put into togerther dry storehouse progress frozen dried together with disk, sterilizing.
The preparation method of the internal styptic sponge of absorbable and degradable, comprises the following steps,
（1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
（2）By step（1）In obtained particle carry out degreasing；
（3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.2%~0.5%, and is added, protease, often 5~10 grams of raw materials and 2~5 grams of protease are added in 100~150 milligrams of 0.2%~0.5% acetic acid solution, is sufficiently mixed mixed It is even, soaked at normal temperatures using magnetic agitation 35~60 it is small when, obtain enzymolysis liquid；
（4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
（5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
（6）By step（5）The collagen solution crude product of acquisition is dialysed with the acetic acid solution of 0.100mol/L~0.155mol/L 45~55 it is small when, then with distilled water dialysis 25~30 it is small when, obtain collagen solution；
（7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injects 8~ 20ml, is then placed in freeze dryer, is freeze-dried to subzero, 35 DEG C~50 DEG C and vacuumize distillation 12~18 it is small when, obtain bee The collagen of nest shape；
（8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:5~20, it is put into centrifuge and centrifuges, goes supernatant to obtain Fill the collagen of biological hemostatic granular powder；
（9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
Relative to the prior art, technique effect of the invention is, the latex film of outer layer is protective layer, by latex film during use After tearing by styptic sponge patch side at wound, after the degradable membrane of collagen outer wrapping is degraded, collagen and collagen The hemostasis granules powder of filling is acted at wound at the same time in peptide backbone, and haemostatic effect is more preferable, and anthemorrhagic speed is fast, improves wound Healing ability.
Embodiment
Biological hemostatic granular powder includes 5~10 parts of Carbazochrome Sodium Sulfonate, 8~15 parts of Radix Notoginseng powder, 5~10 parts of Folium Artemisiae Argyi powder, hairyvein agrimony 15~30 parts, 1~3 part of Diaceturate Ethylenediamine, 12~15 parts of qinghaosu, 90~110 parts of physiological saline.
Biological hemostatic granular powder is prepared according to the quality proportioning in table 1,
1 biological hemostatic granular powder quality proportioning of table
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4
Carbazochrome Sodium Sulfonate 5 8 10 6
Radix Notoginseng powder 10 12 15 5
Folium Artemisiae Argyi powder 8 5 8 10
Hairyvein agrimony 30 20 15 25
Diaceturate Ethylenediamine 1 2 3 2.5
Qinghaosu 13 14 12 15
Physiological saline 90 110 100 90
The preparation method of biological hemostatic granular powder includes,
Step 1：According to mass fraction proportioning weigh Carbazochrome Sodium Sulfonate, Radix Notoginseng powder, Folium Artemisiae Argyi powder, hairyvein agrimony, Diaceturate Ethylenediamine, Qinghaosu, physiological saline；
Step 2：Hairyvein agrimony is put into extractor, adds physiological saline, after being heated to boiling 40min, with a diameter of 0.18 μm Filtering with microporous membrane obtains extracting solution, degerming spare；
Step 3：Carbazochrome Sodium Sulfonate, Radix Notoginseng powder, Folium Artemisiae Argyi powder, Diaceturate Ethylenediamine, qinghaosu add extracting solution, are stirred with glass bar Metal is poured into after mixing uniformly and freezes disk, is covered with preservative film, is put into -90 DEG C of refrigerators and is carried out precooling 24h, is allowed to fully become Solid, after being put into togerther dry storehouse progress frozen dried together with disk, sterilizing.
The biological hemostatic granular powder of gained is spread on wound, daily change dressings 1 time, observe the hemostasia and healing speed of the surface of a wound Rate, data are shown in Table 2,
The hemostasia and healing speed of 2 surface of a wound of table
3d 5d 10d
Tang so-and-so 0.403 0.780 1
Mr. Lin 0.401 0.773 1
Lee 0.43 0.805 1
Mr. Li 0.506 0.812 1
Guo so-and-so 0.388 0.76 1
Mr. Wang 0.396 0.699 1
Money 0.455 0.715 1
Embodiment 1
The preparation method of the internal styptic sponge of absorbable and degradable, comprises the following steps,
（1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
（2）By step（1）In obtained particle carry out degreasing；
（3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.2%~0.5%, and is added, protease, often 5~10 grams of raw materials and 2~5 grams of protease are added in 100~150 milligrams of 0.2%~0.5% acetic acid solution, is sufficiently mixed mixed It is even, soaked at normal temperatures using magnetic agitation 35~60 it is small when, obtain enzymolysis liquid；
（4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
（5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
（6）By step（5）The collagen solution crude product of acquisition is dialysed with the acetic acid solution of 0.100mol/L~0.155mol/L 45~55 it is small when, then with distilled water dialysis 25~30 it is small when, obtain collagen solution；
（7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injects 8~ 20ml, is then placed in freeze dryer, is freeze-dried to subzero, 35 DEG C~50 DEG C and vacuumize distillation 12~18 it is small when, obtain bee The collagen of nest shape；
（8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:5~20, it is put into centrifuge and centrifuges, goes supernatant to obtain Fill the collagen of biological hemostatic granular powder；
（9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
Embodiment 2
The preparation method of the internal styptic sponge of absorbable and degradable, comprises the following steps,
（1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
（2）By step（1）In obtained particle carry out degreasing；
（3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.2%, and is added, protease, every 100 milli Gram 0.2% acetic acid solution in add 8 grams of raw materials and 3 grams of protease, be sufficiently mixed mixing, using magnetic agitation at normal temperatures Soak 45 it is small when, obtain enzymolysis liquid；
（4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
（5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
（6）By step（5）When the collagen solution crude product of acquisition is small with the acetic acid solution dialysis 45 of 0.100mol/L, then to steam When distilled water dialysis 28 is small, collagen solution is obtained；
（7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injects 15ml, is then placed in freeze dryer, is freeze-dried to subzero, 35 DEG C and vacuumize distillation 12 it is small when, obtain cellular collagen Albumen；
（8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:5, it is put into centrifuge and centrifuges, goes supernatant to be filled The collagen of biological hemostatic granular powder；
（9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
Embodiment 3
The preparation method of the internal styptic sponge of absorbable and degradable, comprises the following steps,
（1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
（2）By step（1）In obtained particle carry out degreasing；
（3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.4%, and is added, protease, every 130 milli Gram 0.3% acetic acid solution in add 10 grams of raw materials and 5 grams of protease, be sufficiently mixed mixing, using magnetic agitation at normal temperatures Soak 50 it is small when, obtain enzymolysis liquid；
（4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
（5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
（6）By step（5）When the collagen solution crude product of acquisition is small with the acetic acid solution dialysis 50 of 0.120mol/L, then to steam When distilled water dialysis 25 is small, collagen solution is obtained；
（7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injection 8ml, Be then placed in freeze dryer, be freeze-dried to subzero, 40 DEG C and vacuumize distillation 15 it is small when, obtain cellular collagen；
（8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:20, it is put into centrifuge and centrifuges, goes supernatant to be filled The collagen of biological hemostatic granular powder；
（9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
Embodiment 4
The preparation method of the internal styptic sponge of absorbable and degradable, comprises the following steps,
（1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
（2）By step（1）In obtained particle carry out degreasing；
（3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.5%, and is added, protease, every 150 milli Gram 0.5% acetic acid solution in add 5 grams of raw materials and 2 grams of protease, be sufficiently mixed mixing, using magnetic agitation at normal temperatures Soak 35 it is small when, obtain enzymolysis liquid；
（4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
（5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
（6）By step（5）When the collagen solution crude product of acquisition is small with the acetic acid solution dialysis 55 of 0.155mol/L, then to steam When distilled water dialysis 30 is small, collagen solution is obtained；
（7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injects 20ml, is then placed in freeze dryer, is freeze-dried to subzero, 50 DEG C and vacuumize distillation 18 it is small when, obtain cellular collagen Albumen；
（8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:15, it is put into centrifuge and centrifuges, goes supernatant to be filled The collagen of biological hemostatic granular powder；
（9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
Embodiment 5
The preparation method of the internal styptic sponge of absorbable and degradable, comprises the following steps,
（1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
（2）By step（1）In obtained particle carry out degreasing；
（3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.3%, and is added, protease, every 120 milli Gram 0.4% acetic acid solution in add 6 grams of raw materials and 4 grams of protease, be sufficiently mixed mixing, using magnetic agitation at normal temperatures Soak 60 it is small when, obtain enzymolysis liquid；
（4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
（5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
（6）By step（5）When the collagen solution crude product of acquisition is small with the acetic acid solution dialysis 55 of 0.145mol/L, then to steam When distilled water dialysis 30 is small, collagen solution is obtained；
（7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injects 10ml, is then placed in freeze dryer, is freeze-dried to subzero, 45 DEG C and vacuumize distillation 16 it is small when, obtain cellular collagen Albumen；
（8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:10, it is put into centrifuge and centrifuges, goes supernatant to be filled The collagen of biological hemostatic granular powder；
（9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
The effect of degradable styptic sponge is shown in Table 3,
The degradable styptic sponge analysis of cases of table 3
It can be seen that by above case, degradable styptic sponge hemostasis is very fast, and haemostatic effect is good, and the feelings of wound are not being torn after hemostasis Under condition, wounds streamed blood is less or does not bleed, and degradable styptic sponge will not be permeated by blood.
Above-described is only the preferred embodiment of the present invention, it is noted that for those skilled in the art, Under the premise of general idea of the present invention is not departed from, some changes and improvements can also be made, these should also be considered as the present invention's Protection domain.

Claims (4)

  1. A kind of 1. internal styptic sponge of absorbable and degradable, it is characterised in that：Including cellular skeleton, cellular skeleton is collagen Protein backbone, cellular skeleton is interior to be filled with biological hemostatic granular powder, and cellular skeleton is coated with degradable membrane, can drop Solution film is mixed using seaweed and gelatin, and degradable membrane is coated with latex film.
  2. 2. the internal styptic sponge of absorbable and degradable as claimed in claim 1, it is characterised in that：The biological hemostatic granular powder Including 5~10 parts of Carbazochrome Sodium Sulfonate, 8~15 parts of Radix Notoginseng powder, 5~10 parts of Folium Artemisiae Argyi powder, 15~30 parts of hairyvein agrimony, Diaceturate Ethylenediamine 1~3 part, 12~15 parts of qinghaosu, 90~110 parts of physiological saline.
  3. 3. the internal styptic sponge of absorbable and degradable as claimed in claim 2, it is characterised in that：The biological hemostatic granular powder Preparation method include,
    Step 1：According to mass fraction proportioning weigh Carbazochrome Sodium Sulfonate, Radix Notoginseng powder, Folium Artemisiae Argyi powder, hairyvein agrimony, Diaceturate Ethylenediamine, Qinghaosu, physiological saline；
    Step 2：Hairyvein agrimony is put into extractor, adds physiological saline, after being heated to boiling 40min, with a diameter of 0.18 μm Filtering with microporous membrane obtains extracting solution, degerming spare；
    Step 3：Carbazochrome Sodium Sulfonate, Radix Notoginseng powder, Folium Artemisiae Argyi powder, Diaceturate Ethylenediamine, qinghaosu add extracting solution, are stirred with glass bar Metal is poured into after mixing uniformly and freezes disk, is covered with preservative film, is put into -90 DEG C of refrigerators and is carried out precooling 24h, is allowed to fully become Solid, after being put into togerther dry storehouse progress frozen dried together with disk, sterilizing.
  4. 4. the preparation method of the internal styptic sponge of absorbable and degradable as claimed in claim 1, it is characterised in that：Including following Step,
    （1）Selection skin of mammal is raw material, removes subcutaneous tissue, is ground into particle；
    （2）By step（1）In obtained particle carry out degreasing；
    （3）By step（2）Raw material after degreasing is put into the acetic acid solution that concentration is 0.2%~0.5%, and is added, protease, often 5~10 grams of raw materials and 2~5 grams of protease are added in 100~150 milligrams of 0.2%~0.5% acetic acid solution, is sufficiently mixed mixed It is even, soaked at normal temperatures using magnetic agitation 35~60 it is small when, obtain enzymolysis liquid；
    （4）By step（3）The enzymolysis liquid of acquisition is removed the gred using filtered through gauze, is then filtered by vacuum；
    （5）By step（4）Enzymolysis liquid after filtering is centrifuged using centrifuge, then takes supernatant, and supernatant is collagen egg White solution crude product；
    （6）By step（5）The collagen solution crude product of acquisition is dialysed with the acetic acid solution of 0.100mol/L~0.155mol/L 45~55 it is small when, then with distilled water dialysis 25~30 it is small when, obtain collagen solution；
    （7）By step（6）Collagen solution with the lyophilized culture dish of syringe injection, each culture dish injects 8~ 20ml, is then placed in freeze dryer, is freeze-dried to subzero, 35 DEG C~50 DEG C and vacuumize distillation 12~18 it is small when, obtain bee The collagen of nest shape；
    （8）Biological hemostatic granular powder is added into physiological saline solution into saturated solution, by step（7）In obtained collagen throw Enter in saturated solution, collagen is 1 with saturated solution mass ratio:5~20, it is put into centrifuge and centrifuges, goes supernatant to obtain Fill the collagen of biological hemostatic granular powder；
    （9）By step（8）In after obtained collagen outer wrapping degradable membrane and latex film up to degradable styptic sponge.
CN201711311079.XA 2017-12-11 2017-12-11 A kind of preparation method of the internal styptic sponge of absorbable and degradable Pending CN107982573A (en)

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CN108670498A (en) * 2018-05-25 2018-10-19 海口市人民医院（中南大学湘雅医学院附属海口医院） Intravascular stent and using method thereof

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CN1994481A (en) * 2006-10-10 2007-07-11 辽宁师范大学 Highly effective Chinese medicine-chitosan compound hemostatic material
CN101224311A (en) * 2008-02-01 2008-07-23 北京工业大学 Preparing method of compound collagen hemostatic material
EP2752204A1 (en) * 2008-04-25 2014-07-09 Medtrade Products Ltd. Haemostatic material
CN105343928A (en) * 2015-11-30 2016-02-24 江南大学 Technology for preparing collagen sponge
CN107158448A (en) * 2017-06-01 2017-09-15 苏州乔纳森新材料科技有限公司 A kind of medical bio hemostatic material and preparation method thereof

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CN1994481A (en) * 2006-10-10 2007-07-11 辽宁师范大学 Highly effective Chinese medicine-chitosan compound hemostatic material
CN101224311A (en) * 2008-02-01 2008-07-23 北京工业大学 Preparing method of compound collagen hemostatic material
EP2752204A1 (en) * 2008-04-25 2014-07-09 Medtrade Products Ltd. Haemostatic material
CN105343928A (en) * 2015-11-30 2016-02-24 江南大学 Technology for preparing collagen sponge
CN107158448A (en) * 2017-06-01 2017-09-15 苏州乔纳森新材料科技有限公司 A kind of medical bio hemostatic material and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108670498A (en) * 2018-05-25 2018-10-19 海口市人民医院（中南大学湘雅医学院附属海口医院） Intravascular stent and using method thereof

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