CN107974422A - A kind of bacterial strain administered for heavy metal pollution of soil - Google Patents
A kind of bacterial strain administered for heavy metal pollution of soil Download PDFInfo
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- CN107974422A CN107974422A CN201711402759.2A CN201711402759A CN107974422A CN 107974422 A CN107974422 A CN 107974422A CN 201711402759 A CN201711402759 A CN 201711402759A CN 107974422 A CN107974422 A CN 107974422A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The present invention provides a kind of bacterial strain for having production acid and eliminating heavy metal, and the bacterial strain is AC plants of thiobacillus ferrooxidans (Acidithiobacillus sp.), and deposit number is CGMCC No.14839.Bacterial strain provided by the present invention is used to handle the soil of heavy metal pollution, test result indicates that, select heavy-metal contaminated soil to implement leaching, after the leaching of 3 days, chromium in soil, the removal rate of lead and arsenic these three representative heavy metals are more than 80%.
Description
Technical field
The invention belongs to biotic environment Prevention Technique field, and in particular to a kind of to be used for what heavy metal pollution of soil was administered
Bacterial strain.
Background technology
Mining and metal smelt cause heavy metal constantly to can be discharged into environment, with industrialization to natural biology
The problem of interference of Geochemistry circulation aggravates, heavy metal pollution becomes increasingly severe.The pollution of heavy metal in soil can cause
The heavy damage of soil ecosystem, influences to grow animals and plants system in the present context.Therefore, by heavy metal from environment
Remove to reduce its influence to the ecosystem as far as possible, be all a challenging research in cost and technical elements.
As the microorganism remediation of emerging developing direction in bioremediation technology, then using microorganism (bacterium, algae and
Yeast etc.) heavy metal pollution is mitigated or eliminated compared with physical chemistry restorative procedure, at application environment bioremediation technology
When managing pollutant, final product is mostly material that is harmless, stablizing, does not destroy the soil environment needed for plant growth, can make
Pollutant is removed from environment completely, processing time is short, and small investment, will not produce secondary pollution, easy to operate.But
, it is necessary to which acid adding adjusts the pH to less than 3 of reaction in implementation process, cost is not only increased, can also increase difficulty during operation.
Therefore it is to screen to have to go removing heavy metals ability the problem of present most critical, while can further reduces sour dosage, can
The bacterial strain played function in broad pH systems.
The content of the invention
The object of the present invention is to provide a kind of bacterium for having and removing removing heavy metals and being grown in broad pH scopes system
Strain, the bacterial strain is by the acid production of itself, by the heavy metal dissolution in soil, to achieve the purpose that purification soil.
It is described present invention firstly provides a kind of bacterial strain that removes removing heavy metals and can be grown in broad pH scopes system
Bacterial strain be AC plants of thiobacillus ferrooxidans (Acidithiobacillus sp.), be deposited in court of Beijing on October 27th, 2017
The China Committee for Culture Collection of Microorganisms of No. 3 Institute of Microorganism, Academia Sinica of positive No. 1 institute in area's North Star West Road is common
Microorganism center, deposit number are CGMCC No.14839.
Expand the method for culture above-mentioned bacterial strains, the wherein pH scopes of culture medium are 0.8-7.0.Temperature is 20-50 DEG C；
The present invention also provides a kind of culture medium for being used to expand culture above-mentioned bacterial strains, its composition are as follows：Ammonium sulfate 0.1-
0.5g/L, potassium dihydrogen phosphate 1-3g/L, magnesium sulfate 0.1-0.5g/L, calcium chloride 0.1-0.5g/L, elemental sulfur 0.3-0.5g/L.
Another aspect of the present invention provides a kind of method for handling heavy-metal contaminated soil, is with above-mentioned expansion culture
Bacterium solution handles soil；
A kind of its concrete operations is as follows：
1) seed liquor culture：Culture medium contains following component：Ammonium sulfate 0.1-0.5g/L, potassium dihydrogen phosphate 1-3g/L,
Magnesium sulfate 0.1-0.5g/L, calcium chloride 0.1-0.5g/L, elemental sulfur 0.5g/L, without adjusting pH, cultivates 3 days at 30 degree, completes
The preparation of seed liquor；
2) heavy metal leaching：Heavy-metal contaminated soil is selected to implement leaching processing soil.
Heavy-metal contaminated soil (chromium 590g/m3, lead 610g/m3, zinc 160g/m3) is selected to implement leaching, by 3 days
After leaching, chromium in soil, the removal rate of lead and arsenic these three representative heavy metals are more than 80%.
Embodiment
The present invention is described in detail with reference to embodiment.
Embodiment 1：The screening of bacterial strain and physicochemical property
Inventor is the gram-Negative bacillus that one plant of acidophilus is separated to from the water sample of Tengchong hot spring, is by hot spring sample
Product are obtained, the pH value of culture medium is 2.5,45 with after elemental sulfur continuous passage culture using the method screening of gradient dilution
The bacterial strain that culture obtains the present invention is carried out at DEG C.Acidophilus sulphur bar is accredited as through system identification 16s rRNA gene sequencings
Pseudomonas, is named as AC plants of thiobacillus ferrooxidans (Acidithiobacillus sp.), and Beijing is deposited on October 27th, 2017
The China Committee for Culture Collection of Microorganisms of city Chaoyang District North Star West Road No. 3 Institute of Microorganism, Academia Sinica of No. 1 institute
Common micro-organisms center, deposit number are CGMCC No.14839.
The physicochemical property for the AC strains that the present invention screens is as follows：
1) morphological feature of bacterial strain
Bacterial strain is rod-shaped, its thalline is about 0.4-0.8 μm, and thalline width is 0.2-0.4 μm.Coloration result is shown as removing from office
Lan Shi negative bacteriums, no gemma, thalline do not have pod membrane, without the raw flagellum in end.
2) condition of culture
Grown in thiobacillus ferrooxidans' AC liquid medium withins, 45 DEG C of trainings on the culture medium of bacterial liquid 9k addition elemental sulfurs
Support 72 it is small when, can muddy growth, do not form mycoderm, no deposited phenomenon.The growth temperature range of thiobacillus ferrooxidans AC is 25~55
DEG C, detected, found at 45 DEG C by cell count, cell passage repoductive time is most short, is the optimum growth temp of the bacterium.
Embodiment 2：The effect of bacterial strain
1st, bench-scale testing
1) seed liquor is prepared
9k culture mediums are selected, add the elemental sulfur of 0.1% (W/V), at 30 DEG C, 180rpm is enlarged bacterium culture,
Stop culture when value to OD600 is 0.3-0.6, seed liquor is made.
2) processing of sludge containing heavy metal
1kg sludge is deposited in fermentation vat or fermentation tank, then adds 9k culture mediums, makes sludge in the medium
Mass concentration is 10%, is then added to cultured bacterium solution in 20L fermentation tanks in 1: 10 ratio, controlling reaction temperature
For 30 DEG C, stirring fermentation 48-72h.In processing procedure, air is passed through into system with per minute 1 to 10ml speed.Leach
During operation and after, with National Standard Method measure leachate in heavy metal content, the results show that select it is heavy metal-polluted
Contaminate soil (chromium 590g/m3, lead 610g/m3, arsenic 160g/m3) and implement leaching, after the leaching of 3 days, the removal of chromium in soil
Rate is more than 85%, lead more than 95%, more than arsenic 90%.The above results show that the bacterial strain of the present invention has degraded weight well
The effect of metal.
2nd, middle trial operation
1) seed liquor is prepared
9k culture mediums are selected, add the elemental sulfur of 0.1% (W/V), at 45 DEG C, 180rpm is enlarged bacterium culture,
Stop culture when value to OD600 is 0.5-1.0, seed liquor is made.
2) processing of sludge containing heavy metal
2 tons of sludge are deposited in fermentation vat or fermentation tank, then add 9k culture mediums, make sludge in the medium
Mass concentration is 10%, is then added to cultured bacterium solution in fermentation vat or fermentation tank in 1: 10 ratio, then with following
Ring water pipe or vapour heating are to 30 DEG C, stirring fermentation 48-72h.In processing procedure, with per minute 1 to 10ml speed to body
Air is passed through in system.During leaching operation and after, with National Standard Method measure leachate in heavy metal content, as a result
It has been shown that, selects heavy-metal contaminated soil (chromium 590g/m3, lead 610g/m3, arsenic 160g/m3) to implement leaching, by the leaching of 3 days
Afterwards, the removal rate of chromium is more than 80% in soil, lead more than 90%, arsenic more than 85%.The above results show the bacterium of the present invention
Strain has the effect of degraded heavy metal well.
Claims (7)
1. a kind of bacterial strain, it is characterised in that the deposit number of the bacterial strain is CGMCC No.14839.
2. application of the bacterial strain in production acid or elimination heavy metal described in claim 1.
A kind of 3. method for expanding the bacterial strain described in culture claim 1, it is characterised in that the method, wherein culture medium
PH scopes be 0.8-7.0.Temperature is 20-50 DEG C.
4. the method as described in claim 1, it is characterised in that the culture medium, its composition are as follows：Ammonium sulfate 0.1-
0.5g/L, potassium dihydrogen phosphate 1-3g/L, magnesium sulfate 0.1-0.5g/L, calcium chloride 0.1-0.5g/L, elemental sulfur 0.3-0.5g/L.
A kind of 5. method for handling heavy-metal contaminated soil, it is characterised in that the method is described in usage right requirement 1
Bacterial strain handles soil.
6. method as claimed in claim 5, it is characterised in that the method is the expansion with the bacterial strain described in claim 1
The bacterium solution cultivated greatly handles soil.
7. method as claimed in claim 6, it is characterised in that the method includes the steps：
1) seed liquor culture：Culture medium contains following component：Ammonium sulfate 0.1-0.5g/L, potassium dihydrogen phosphate 1-3g/L, sulfuric acid
Magnesium 0.1-0.5g/L, calcium chloride 0.1-0.5g/L, elemental sulfur 0.5g/L, without adjusting pH, cultivates 3 days at 30 degree, completes seed
The preparation of liquid；
2) heavy metal leaching：Heavy-metal contaminated soil is selected to implement leaching processing soil.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711402759.2A CN107974422A (en) | 2017-12-22 | 2017-12-22 | A kind of bacterial strain administered for heavy metal pollution of soil |
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| CN201711402759.2A CN107974422A (en) | 2017-12-22 | 2017-12-22 | A kind of bacterial strain administered for heavy metal pollution of soil |
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Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935621A (en) * | 2010-02-04 | 2011-01-05 | 山东大学 | Acidithiobacillus ferrooxidans genetic engineering bacteria and use thereof |
| CN102127516B (en) * | 2010-12-20 | 2012-07-04 | 中南大学 | Microbacterium sp.T3 with tolerance on heavy metals and application thereof |
| CN102527713B (en) * | 2011-12-29 | 2013-05-15 | 中南大学 | Biological leaching restoring method for heavy metal polluted soil |
| CN103114039A (en) * | 2013-02-22 | 2013-05-22 | 广州天壤生物科技有限公司 | Biological agent capable of repairing heavy metal contaminated soil and soil remediation method |
| CN103232953B (en) * | 2013-03-26 | 2014-12-31 | 华南理工大学 | Acidithiobacillus ferrooxidans and applications thereof |
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| CN106906173A (en) * | 2017-05-05 | 2017-06-30 | 南京工业大学 | Thiobacillus thiooxidans and application thereof in heavy metal removal |
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2017
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| CN102127516B (en) * | 2010-12-20 | 2012-07-04 | 中南大学 | Microbacterium sp.T3 with tolerance on heavy metals and application thereof |
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Application publication date: 20180501 |