CN107974393B - Heating device applied to nucleic acid extraction system - Google Patents
Heating device applied to nucleic acid extraction system Download PDFInfo
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- CN107974393B CN107974393B CN201810015465.2A CN201810015465A CN107974393B CN 107974393 B CN107974393 B CN 107974393B CN 201810015465 A CN201810015465 A CN 201810015465A CN 107974393 B CN107974393 B CN 107974393B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
The invention relates to a heating device applied to a nucleic acid extraction system, which mainly comprises a heating aluminum strip, a heating aluminum strip fixing support plate and a push rod, wherein the heating aluminum strip is fixed on the heating aluminum strip fixing support plate, the push rod is connected with a front door of the whole nucleic acid extraction system, when the front door is opened, the push rod is pushed to enable the heating aluminum strip fixing support plate to move downwards, and a user can move into a reagent plate in a translation manner; when the front door was closed, the spring restoring force on the fixed extension board of heating aluminium strip made it shift up, made heating aluminium strip and reagent board bottom closely laminate to improve the heat transfer efficiency of heating part. The device of the invention can facilitate the user to place and take out the reagent plate, effectively reduce the influence of human factors and improve the efficiency and safety of nucleic acid extraction.
Description
Technical Field
The invention relates to a heating device applied to a nucleic acid extraction system, and belongs to the fields of molecular diagnosis and biomedicine.
Background
In the fields of genetic engineering and protein engineering, nucleic acid molecules are the main research objects, the separation and purification of nucleic acid are the basic technologies of nucleic acid-based molecular diagnosis research, and the accurate detection of subsequent nucleic acid is a prerequisite for obtaining nucleic acid with higher purity from biological samples.
The nucleic acid extraction technology of the paramagnetic particle method adopts a nanotechnology to improve and modify the surfaces of superparamagnetic nanoparticles to prepare superparamagnetic silicon carbide nanobeads, and the nanobeads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface. The superparamagnetic property of the magnetic beads is utilized, under the action of an external magnetic field, DNA or RNA can be separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like, and the process is simple, rapid and easy to automate. At present, the heating part in most nucleic acid purification systems has low heat transfer efficiency due to small contact area with a reagent plate, loose contact and large air gap, and the actual reagent reaction temperature often does not reach the temperature set by a customer through a human-computer interaction interface, so that the nucleic acid extraction effect is poor, and the accuracy of an experimental result is directly influenced. In addition, the combination of the reagent plate and the heating part, which is common in the nucleic acid extraction system, is to lift the reagent plate upwards and then take it out, in the process, the reagent may be shaken out of the reagent plate due to improper control of the lifted force, which may cause cross contamination of the sample and safety problems for the experimenters.
In order to solve the above problems, the present invention provides a heating device applied to a nucleic acid extraction system, which can improve the heat transfer efficiency of a heating part, facilitate the placement and removal of reagent plates, effectively reduce the influence of human factors, and improve the efficiency and safety of nucleic acid extraction.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the heating device applied to the nucleic acid extraction system, which improves the heat transfer efficiency of the heating part, is convenient for placing and taking out the reagent plate, effectively reduces the influence of human factors, and improves the efficiency and the safety of nucleic acid extraction.
In order to realize the purpose and the advantages of the invention, the invention is realized by the following technical scheme:
a heating device applied to a nucleic acid extraction system mainly comprises: heating aluminum strips, a supporting plate for fixing the heating aluminum strips and a push rod.
One side of the heating aluminum strip is a smooth plane, and the other side of the heating aluminum strip is provided with a plurality of U-shaped grooves matched and matched with the bottom of the reagent plate.
The heating aluminum strip fixing support plate is provided with a plurality of rectangular strip holes, the length and the width of each strip hole are matched with the length and the width of each heating aluminum strip, and each heating aluminum strip is fixed in each strip hole; and two parallel inclined slotted holes are formed in the side surface of the heating aluminum strip fixing support plate.
The push rod is connected with a front door of the nucleic acid extraction system through a movable hinge, an inclined block is fixed below the front door, the push rod is erected in the two square grooves, and the inclined block is in contact with one end of the push rod. The inner side surface of the push rod is provided with two protruding short rods, the distance between the two protruding short rods is the same as the distance between the parallel inclined slotted holes on the side surface of the heating aluminum strip fixing support plate, and the thickness of the protruding short rods is matched with the aperture of the inclined slotted holes and can be just inserted into the inclined slotted holes.
And screws are sleeved at four corners of the heating aluminum strip fixing support plate, and springs are sleeved on the screws. The spring is defined between the base plate and the screw of the overall system.
Further, the number of the heating aluminum strips is at least 4.
Preferably, at least 4 rectangular strip holes are formed in the heating aluminum strip fixing support plate.
When the front door is opened, the inclined block pushes and presses the push rod, and the push rod moves inwards along the square groove to drive the protruding short rod on the push rod to move. The protruded short rod is inserted into the inclined slotted hole of the heating aluminum strip fixing support plate, so that the protruded short rod can move to the inclined upper end along the inclined slotted hole, and the whole heating aluminum strip fixing support plate moves downwards by the downward pressure generated on the inclined slotted hole to drive the screw to move downwards to compress the spring; when the front door is closed, the pushing force of the push rod is removed by the inclined block, and due to the resilience force of the spring, the screw and the heating aluminum strip fixing support plate are driven to move upwards, so that the protruding short rod on the push rod moves to the inclined lower end of the inclined slotted hole.
Compared with the prior art, the invention has the beneficial effects that: the high-efficiency heat transfer can be realized by matching with a conventional 96 Kong Shiji plate on the market, the nucleic acid extraction efficiency is improved, the placement and the taking out of a user reagent plate are facilitated, and the accuracy of an experimental result and the safety of an experimental process are ensured.
Drawings
FIG. 1 is a side sectional view of the present invention.
Fig. 2 is a diagram showing an open state of the front door of the present invention.
Fig. 3 is a diagram of the closed state of the front door of the present invention.
FIG. 4 is a diagram showing the nucleic acid isolation system according to the present invention with the front door opened.
FIG. 5 is a diagram showing the combination of the nucleic acid isolation system and the front door of the present invention when the front door is closed.
In the figure: 1-heating aluminum strip, 2-heating aluminum strip fixing support plate, 201-inclined slotted hole, 3-screw, 4-spring, 5-push rod, 501-protruding short rod, 6-front door, 601-inclined block, 7-reagent plate and 8-system bottom plate.
Detailed Description
The invention is further explained below with reference to examples and figures 1-5 so that a person skilled in the art can carry out the invention with reference to the description. The following examples are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1, the present invention mainly includes: heating aluminum strip 1, heating aluminum strip fixed support plate 2, screw 3 and spring 4 and push rod 5.
As shown in FIGS. 2-5, before the nucleic acid extraction experiment, the reagent plate 7 is filled with the reagents and samples required by the experiment, when the front door 6 is opened, the inclined block 601 pushes the push rod 5, and the push rod 5 moves inwards along the square groove to drive the short protruding rod 501 on the push rod to move. The protruding short rod 501 is inserted into the inclined slot 201 of the heating aluminum strip fixing support plate 2, so that the protruding short rod 501 moves to the inclined upper end along the inclined slot 201, as shown in fig. 2 and 4, the whole heating aluminum strip fixing support plate 2 moves downwards by the downward pressure generated on the inclined slot 201, the screw 3 is driven to move downwards to compress the spring 4, at this time, the heating aluminum strip fixing support plate 2 with the heating aluminum strip 1 moves downwards to the lower side of the system bottom plate 8, the area of the system bottom plate 8 where the reagent plate 7 is placed is flat, and the reagent plate 7 can be pushed into the system from the outside horizontally along the arrow direction in fig. 4 to be fixed.
When the front door 6 is closed, the pushing force of the push rod 5 is released by the inclined block 601, and due to the resilience force of the spring 4, the screw 3 and the heating aluminum strip fixing support plate 2 are driven to move upwards, so that the protruding short rod 501 on the push rod 5 moves to the inclined lower end of the inclined slotted hole 201, as shown in fig. 3 and 5, at the moment, the heating aluminum strip 1 protrudes out of the system bottom plate 8 and is tightly attached to the bottom of the reagent plate 7, and the heat transfer efficiency in the experimental process is improved.
After the experiment, open qianmen 6, heating aluminium strip 1 descends, and reagent board 7 can follow system bottom plate 8 level and shift out, and whole process resistance is very little, can not cause the reagent oscillation in the reagent board 7 to spill, has guaranteed the accuracy of experimental result and experimenter's security.
Claims (2)
1. A heating device applied to a nucleic acid extraction system, characterized in that: mainly comprises a heating aluminum strip, a heating aluminum strip fixing support plate and a push rod;
the heating aluminum strip fixing support plate is provided with a plurality of rectangular strip holes, the length and the width of each strip hole are matched with the length and the width of each heating aluminum strip, and one heating aluminum strip is fixed in each strip hole; two parallel inclined slotted holes are formed in the side face of the heating aluminum strip fixing support plate;
two protruded short rods are arranged on the inner side surface of the push rod, the distance between the two protruded short rods is the same as the distance between the parallel inclined slotted holes on the side surface of the heating aluminum strip fixing support plate, the thickness of the protruded short rods is matched with the aperture of the inclined slotted holes, and the protruded short rods can be just inserted into the inclined slotted holes;
one surface of the heating aluminum strip is a smooth plane, and the other surface of the heating aluminum strip is provided with a plurality of U-shaped grooves matched with the bottom of the reagent plate;
the push rod is connected with a front door of the nucleic acid extraction system through a movable hinge, an inclined block is fixed below the front door, the push rod is erected in the two square grooves, and the inclined block is in contact with one end of the push rod.
2. The heating apparatus according to claim 1, wherein: the four corners of the heating aluminum strip fixing support plate are sleeved with screws, the screws are sleeved with springs, and the springs are limited between the bottom plate and the screws of the whole system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810015465.2A CN107974393B (en) | 2018-01-08 | 2018-01-08 | Heating device applied to nucleic acid extraction system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810015465.2A CN107974393B (en) | 2018-01-08 | 2018-01-08 | Heating device applied to nucleic acid extraction system |
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| CN107974393A CN107974393A (en) | 2018-05-01 |
| CN107974393B true CN107974393B (en) | 2023-03-24 |
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| CN201810015465.2A Active CN107974393B (en) | 2018-01-08 | 2018-01-08 | Heating device applied to nucleic acid extraction system |
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Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080268529A1 (en) * | 2004-06-02 | 2008-10-30 | Arkray, Inc. | Container for Nucleic Acid Amplification, Nucleic Acid Preparation Kit and Nucleic Acid Analyzer |
| WO2014149268A1 (en) * | 2013-03-19 | 2014-09-25 | Life Technologies Corporation | Thermal cycler cover |
| CN203866297U (en) * | 2013-11-01 | 2014-10-08 | 艾康生物技术(杭州)有限公司 | Clamp for nucleic acid extraction instrument |
| CN204474683U (en) * | 2015-03-05 | 2015-07-15 | 东南大学 | A kind of full-automatic heat circulating instrument heat lid |
| CN104630057B (en) * | 2015-03-05 | 2016-08-17 | 东南大学 | A kind of full-automatic heat circulating instrument heat lid |
| WO2017154349A1 (en) * | 2016-03-10 | 2017-09-14 | パナソニックＩｐマネジメント株式会社 | Nucleic acid extraction device, nucleic acid extraction unit, and nucleic acid extraction method |
| CN205699822U (en) * | 2016-06-08 | 2016-11-23 | 南京全景环保科技有限公司 | A kind of high-efficiency device for purifying waste gas |
| CN205786519U (en) * | 2016-06-25 | 2016-12-07 | 昌宁德康生物科技有限公司 | The manually gas chromatogram hydrogen flame ionization detector rapid feeding device of charging |
| CN107442875B (en) * | 2017-10-11 | 2018-12-21 | 广东工业大学 | A kind of simple clamped device of con-rod fracture splitting slot processing |
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Address after: 518000 1002, building 1, Senyang Electronic Technology Park, west area, Guangming Gaoxin Park, Tianliao community, Yutang street, Guangming District, Shenzhen, Guangdong Province Patentee after: SHENZHEN LEMNISCARE MEDICAL TECHNOLOGY CO.,LTD. Address before: 518000 901, East Block, Coast Times Apartment, No. 12069, Shennan Avenue, Nantou Street, Nanshan District, Shenzhen, Guangdong Province Patentee before: SHENZHEN LEMNISCARE MEDICAL TECHNOLOGY CO.,LTD. |
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