CN107970456A - Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety - Google Patents
Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety Download PDFInfo
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- CN107970456A CN107970456A CN201711107859.2A CN201711107859A CN107970456A CN 107970456 A CN107970456 A CN 107970456A CN 201711107859 A CN201711107859 A CN 201711107859A CN 107970456 A CN107970456 A CN 107970456A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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Abstract
Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety, belongs to biology and optical field.Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety.The particle size range of black phosphorus nano particle is 10 100nm, available in cell or biological living.
Description
Technical field
The invention belongs to biology and optical field, is related to Near-infrared Double photon contrast agent and New Two Dimensional material and its answers
With, and in particular to the two-photon contrast agent available for bio-imaging；The Near-infrared Double photon contrast agent bio-toxicity is small, easily
Degraded, single photon and two-photon fluorescence luminous efficiency are high.
Background technology
Due to the birth of femtosecond pulse laser, for example double light of the microtechnic more advantageous than Confocal laser endomicroscopy
Sub- microscopy is come into being.Exciting light used in two-photon technology is near infrared band, the optical wavelength of near infrared band, carrying
Little energy, therefore to biological tissue injure it is minimum；And the penetration power of near infrared light (wave-length coverage is in 780-3000nm) is strong,
Therefore the near infrared light of specific band is referred to as the optical window of biological tissue, and the electromagnetic wave in the range of optical window is due to life
Thing tissue each component is small in the absorption of secondary wave band, so secondary wave band can realize that penetration depth reaches maximum in biological tissue.Though
The scope of the near-infrared optical window of right biological tissue can be slightly different because of different tissue, but substantially scope be all
Between 600-1400nm.
Compared to single photon microscope, the fluorescence molecule outside Two Photon Fluorescence focal plane will not be excited, and photobleaching is small；
Since near-infrared wavelength is grown, Two Photon Fluorescence phototoxicity is small；Two-photon excitation light source and cell autofluorescence induction light source
Wave band is misaligned, effectively can significantly avoid cell autofluorescence.Thus two photon imaging technology becomes a popular direction.
Phosphorus is one of essential trace element in human body, there is the presence of P elements in all cells, and phosphorus participates in
Almost all of biochemical reaction in human body, important role is also play in the health of skeleton and tooth is maintained.Just
It is such a so active element in human body, ensure that the high biological compatibility and non-toxic nature of black phosphorus.It is existing
Common two-photon dyestuff, such as rhodamine, have certain cytotoxicity.And black phosphorus is then a kind of highly safe material,
The product phosphate of black phosphorus degraded is also the universal material being present in human body, can simple and effective metabolism discharge human body.
Thus black phosphorus is a kind of preferably bio-safety two-photon contrast agent.
The content of the invention
For the further development of the prior art, it is black that the problem to be solved in the present invention is to provide a kind of bio-safety contrast agent
Application of the phosphorus nano particle in two-photon biological imaging.
Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety.
Particle size range as the black phosphorus nano particle of the two-photon contrast agent of bio-safety is 10-100nm, preferably 30-
50nm。
It is preferred that the contrast agent of the present invention that be used for two-photon biological imaging with biological safety is by black phosphorus crystal system
Standby is nano material, can be as the contrast agent for the two-photon biological imaging in cell or biological living.
Black phosphorus then has four kinds of different molecular structures：Undefined structure；Simple cubic structure；Rhombohedral system and orthorhombic
System.The present invention
Black phosphorus nano particle as the two-photon contrast agent of bio-safety preferably uses liquid phase stripping method, liquid phase stripping method
Obtained nanoparticle suspension is in a solvent.Preferred solvent is ultra-pure water.
Contrast agent of the present invention that be used for two-photon biological imaging with biological safety can be realized in living cells
Two photon imaging.
Contrast agent of the present invention that be used for two-photon biological imaging with biological safety can realize living animal
Interior angiogram.
The exciting light of two-photon contrast agent black phosphorus nano particle is near infrared band.The contrast agent can mark to be removed into the cell
Extranuclear all sites.The contrast agent can react vascular morphology and label vascular position with label vascular.The contrast agent can
Mark and be imaged for living animal.The contrast agent can be used for animal tissue sections mark and imaging.
It is demonstrated experimentally that the contrast agent black phosphorus of the present invention that be used for two-photon biological imaging with biological safety holds very much
Quick to realize easily by cell membrane, safety, intuitively fluoroscopic examination provides technical support.
It is demonstrated experimentally that the contrast agent black phosphorus of the present invention that be used for two-photon biological imaging with biological safety is logical
Circulation is come within 5 minutes crossed after being injected intravenously, can detect that signal realizes living animal blood vessel in the blood vessels
Two photon imaging.
The beneficial effects of the invention are as follows：By experiment screening, the present invention establishes a kind of to be used for biological safety
The contrast agent of two-photon biological imaging, i.e. black phosphorus.Black phosphorus nano particle of the present invention, which has, easily to be prepared, and excitation ability is low, raw
The characteristics of thing good compatibility.Further it is demonstrated experimentally that the black phosphorus nano particle of the present invention has nontoxicity, and good cell
The features such as membrane permeability.And black phosphorus nano particle of the present invention also has Photothermal characterisation, light dynamic characteristics and optoacoustic are special
Property, has huge application potential realize multi-functional in terms of the therapeutic alliance of the relevant diseases such as cancer.
Brief description of the drawings
A kind of molecular structure models of black phosphorus nano particle of Fig. 1.
Fig. 2：In the two-photon excitation human umbilical vein endothelial cells two-photon cell imaging photo of 1125nm wavelength.A, b,
C is and black phosphorus nano particle cultivate together 24 it is small when after Human umbilical vein endothelial cells (experimental group) figure a be photograph under light field
Piece, figure b are the two-photon signal being collected into after 460-500nm filter plates filter, and scheme c to be received after the filtering of 520-560nm filter plates
The two-photon signal collected.D, e, f be do not cultivated together with black phosphorus nano particle 24 it is small when after Human umbilical vein endothelial cells
(control group), figure d are the photo under light field, and figure e is the two-photon signal being collected into after 460-500nm filter plates filter, and figure f is
The two-photon signal being collected into after the filtering of 520-560nm filter plates.
Fig. 3：It is 400 μ g/mL black phosphorus nano particles to have injected 500 μ l concentration to experimental group under 910nm two-photon excitations
Mouse ear blood vessel two photon imaging, signal collection scope is 500-550nm.
Fig. 4：The two-photon of the mouse ear blood vessel of 500 μ l PBS has been injected to control group under 910nm two-photon excitations
Imaging, signal collection scope is 500-550nm.
Embodiment
Embodiment described below is for the present invention for example, but being not limited to the scope of the present invention.Led for this technology
For the those of ordinary skill in domain, on the premise of principle of the embodiment of the present invention is not departed from, some improvement can also be made, such as
The optimization of excitation wavelength and launch wavelength scope, these improvement are also considered as the protection domain of present example.Following embodiments are outstanding
It selects rhombic system or/and undefined structure.
Embodiment 1：
The two-photon cell imaging of the safe two-photon contrast agent black phosphorus nano particle of new bio
Black phosphorus crystal after grinding is prepared as nano-scale particle with liquid phase stripping method, the black phosphorus nanometer being prepared
Particle suspends in a suitable solvent.It is most simple and do not have virose solvent to select ultra-pure water,
Adjust black phosphorus nano granule suspension concentration.By adjust concentration black phosphorus nano granule suspension and cell together
Amount of time is cultivated, two photon imaging is carried out to living cells after cell is gulped down into appropriate black phosphorus.
Embodiment 2
The two-photon animal imaging of bio-safety two-photon contrast agent black phosphorus nano particle
Black phosphorus nano granule suspension is adjusted to suitable concentration.By ready mouse anesthesia, treat that mouse loses consciousness
The black phosphorus nano granule suspension for mixing up concentration is injected intravenously mouse afterwards.Will note on the premise of mouse is not injured
Mouse after penetrating is fixed on Two Photon Fluorescence platform, and to its ear, blood vessel is imaged.
Claims (6)
1. application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety.
2. application of the black phosphorus nano particle described in accordance with the claim 1 as the two-photon contrast agent of bio-safety, its feature
It is, the particle size range of black phosphorus nano particle is 10-100nm.
3. application of the black phosphorus nano particle described in accordance with the claim 1 as the two-photon contrast agent of bio-safety, its feature
It is, the particle size range of black phosphorus nano particle is 30-50nm.
4. application of the black phosphorus nano particle described in accordance with the claim 1 as the two-photon contrast agent of bio-safety, its feature
It is, the exciting light of two-photon contrast agent black phosphorus nano particle is near infrared band.
5. application of the black phosphorus nano particle described in accordance with the claim 3 as the two-photon contrast agent of bio-safety, its feature
It is, can be as the two-photon biological mark or the contrast agent of imaging in cell or biological living.
6. according to application of the black phosphorus nano particle described in claim 5 as the two-photon contrast agent of bio-safety, its feature
It is, is all sites except the nucleus in cell or biological living.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711107859.2A CN107970456A (en) | 2017-11-10 | 2017-11-10 | Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711107859.2A CN107970456A (en) | 2017-11-10 | 2017-11-10 | Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety |
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| CN201711107859.2A Pending CN107970456A (en) | 2017-11-10 | 2017-11-10 | Application of the black phosphorus nano particle as the two-photon contrast agent of bio-safety |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267201A (en) * | 2016-08-26 | 2017-01-04 | 深圳先进技术研究院 | A kind of black phosphorus of polymer wrapped and preparation method and application |
| CN107043093A (en) * | 2017-04-21 | 2017-08-15 | 北京工业大学 | A kind of method that black phosphorus nano grain surface wraps up mesoporous silicon oxide |
-
2017
- 2017-11-10 CN CN201711107859.2A patent/CN107970456A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267201A (en) * | 2016-08-26 | 2017-01-04 | 深圳先进技术研究院 | A kind of black phosphorus of polymer wrapped and preparation method and application |
| CN107043093A (en) * | 2017-04-21 | 2017-08-15 | 北京工业大学 | A kind of method that black phosphorus nano grain surface wraps up mesoporous silicon oxide |
Non-Patent Citations (2)
| Title |
|---|
| WEI GU ET AL: "Fluorescent black phosphorus quantum dots as label-free sensingprobes for sensingprobes for evaluation of acetylcholinesterase activity", 《SENSORS AND ACTUATORS B》 * |
| YING TENG YEW,ET AL: "Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection", 《MATER. CHEM. FRONT》 * |
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Application publication date: 20180501 |