CN107970227A - A kind of preparation method of casein/sodium alginate composite microcapsule - Google Patents
A kind of preparation method of casein/sodium alginate composite microcapsule Download PDFInfo
- Publication number
- CN107970227A CN107970227A CN201711065031.5A CN201711065031A CN107970227A CN 107970227 A CN107970227 A CN 107970227A CN 201711065031 A CN201711065031 A CN 201711065031A CN 107970227 A CN107970227 A CN 107970227A
- Authority
- CN
- China
- Prior art keywords
- casein
- sodium alginate
- solution
- riboflavin
- capsule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5052—Proteins, e.g. albumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
- A61K31/525—Isoalloxazines, e.g. riboflavins, vitamin B2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5036—Polysaccharides, e.g. gums, alginate; Cyclodextrin
Landscapes
- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a kind of preparation method of casein/sodium alginate composite microcapsule, prepares casein solution, then weighs sodium alginate powder, and mechanical agitation, makes it be completely dissolved in casein solution and form uniform casein/mixed solution of sodium alginate at room temperature；Prepare CaCl2Solution, at room temperature magnetic agitation obtain the solution of water white transparency as curing coagulating bath；Casein/mixed solution of sodium alginate is added dropwise to CaCl2Cure in coagulating bath, obtain plural gel micro-capsule；Micro-capsule is collected by filtration.And embedded by model drug of riboflavin；Using sodium alginate and casein as compound wall materials, core is made with riboflavin, obtains the microcapsules of biocompatibility, can be used for the bioactive substance for conveying a variety of sensitiveness.Micro-capsule embedding rate higher compared with conventional microcapsules, there is good sustained release performance under the conditions of stomach is simulated, and used casein can discharge several functions polypeptide after disassembly, its preparation process condition is gentle, and operating condition is safe and simple, be conducive to mass produce.
Description
Technical field
The present invention relates to a kind of preparation method of casein/sodium alginate composite micro-capsule, belong to using albumen as wall material system
The technical field of biological material of standby microcapsules.
Background technology
Microcapsules technology refers to some using natural or synthetic high molecular material with sensitiveness, reactivity or waves
The solid, liquid, gas body material of hair property wraps up, and forms with semi permeability or seal the technology of cyst membrane.The preparation method of microcapsules
And core wall material huge number, it is possible to achieve the cladding of various active material, has been widely used in medicine and biotechnology at present
Deng field.The one kind of sodium alginate as wall material, can be cross-linked to form insoluble gel layer with divalence or polyvalent cation, reach encapsulation
The purpose of medicine core.In the preparation of microcapsules, in order to strengthen the feature of capsule wall material, often some polymer are added
Into sodium alginate system.Such as：Chitosan is combined with sodium alginate, this method enhances wall material to a certain extent
Adhesiveness and micro-capsule stability, but its pattern is not homogeneous enough, and there are alligatoring, easily loses core[1].Separately
Outside, the research of modified starch is added also in sodium alginate system, this method has modified Polyphenols core material in acidity
Release in medium, but the intensity of micro-capsule and stability are not good enough[2].In addition there are sodium alginate and whey separation egg
The research combined in vain, this method prepare it is microsphere supported there is good biocompatibility, live available for the oral class of load
Property material, but its application range is narrow, core material is limited[3].Therefore, it is necessary to select New Wall Material to make it in microcapsules field
Meet many requirements.
Casein is the highest protein of content in mammal lotion, accounts for the 80% of lactoprotein.Casein is by αs1- junket
Albumen, αs2The complicated micella molecule that four kinds of phosphorylated protein aggregations such as-casein, beta-casein, κ-casein form.They can
To provide abundant amino acid for newborn baby and human body, moreover it is possible to it is dense to improve solution calcium ion using its special micellar structure
Degree, prevents mammary gland lesion caused by calcification.In addition, casein via protease hydrolytic, can release various active polypeptide,
So as to improve the immune and disease resistance of body.Due to the special structural form of casein and physicochemical properties, become
The natural carrier of bioactive substance.Sodium alginate possess with the cured characteristic of bivalent metal ion temporary cross-link, be as sharp
Hole-solidification bath method prepares the optimal wall material of microcapsules.But it yet there are no and be combined casein with sharp with sodium alginate
Hole-solidification bath method prepares the report of microcapsules.Present invention seek to address that some problems occurred in existing microcapsules technology, if
A kind of preparation method of casein and sodium alginate composite micro-capsule has been counted, and has been used for medicament slow release.
Bibliography:
[1]O,Jolliffe I G,Hampson F C.The enhancement of the
bioadhesive properties of calcium alginate gel beads by coating with
chitosan.International Journal of Pharmaceutics,1998,175(2):237-246.
[2]López Córdoba A,Deladino L,Martino M.Effect of starch filler on
calcium-alginate hydrogels loaded with yerba mate antioxidants.Carbohydrate
Polymers,2013,95(1):315-323.
[3]Chen L,Subirade M.Alginate-whey protein granular microspheres as
oral delivery vehicles for bioactive compounds.Biomaterials,2006,27(26):4646-
4654.
The content of the invention
It is an object of the present invention to provide a kind of preparation method of casein/sodium alginate composite micro-capsule.Including casein/sea
The preparation method of mosanom composite microcapsule, and casein/sodium alginate composite microcapsule embedding molds medicine riboflavin core
Using.
Concrete technical scheme is as follows：
The preparation method of a kind of casein/sodium alginate composite microcapsule, it is characterized in that step is as follows：
1) casein solution is prepared, then weighs sodium alginate powder, mechanical agitation, makes it be completely dissolved in junket egg at room temperature
Uniform casein/mixed solution of sodium alginate is formed in white solution；
2) CaCl is prepared2Solution, at room temperature magnetic agitation obtain the solution of water white transparency as curing coagulating bath；
3) above-mentioned casein/mixed solution of sodium alginate is added dropwise to CaCl2Cure in coagulating bath, it is micro- to obtain plural gel
Capsule；After curing 3-60min, micro-capsule is collected by filtration, and use distilled water flushing.
Wherein：The mass concentration of casein solution is 1-6% (%, w/v), and sodium alginate is mixed in casein/sodium alginate
The mass concentration closed in solution is 1.75~3.25% (%, w/v), CaCl2Mass concentration in its coagulating bath is 0.4-
0.8% (%, w/v).
Sodium alginate is in casein/mixed solution of sodium alginate in the casein/sodium alginate composite microcapsule
Mass concentration is 2.8% (w/v), CaCl2Mass concentration in its coagulating bath is 0.53% (w/v).
The rate of addition of step 3) be preferably with 90 drop/point.
The application of casein prepared by the present invention/sodium alginate composite microcapsule embedding molds medicine riboflavin core, first
Casein solution is prepared, then weighs sodium alginate powder, mechanical agitation, makes it be completely dissolved in shape in casein solution at room temperature
Into uniform casein/mixed solution of sodium alginate；Riboflavin is weighed again and adds above-mentioned mixed solution, is formed comprising riboflavin
Casein/mixed solution of sodium alginate；CaCl is prepared again2Solution, at room temperature magnetic agitation obtain the solution conduct of water white transparency
Cure coagulating bath.
Riboflavin/casein/mixed solution of sodium alginate is added dropwise to CaCl2In coagulating bath, plural gel micro-capsule is obtained；Gu
After changing 3-60min, micro-capsule is collected by filtration, and with distilled water flushing, casein/alginic acid of obtained embedding molds medicine riboflavin
Sodium microcapsules.
The drugloading rate of model drug riboflavin is preferably 3.25mg/ml in the microcapsules.
The present invention makees core with riboflavin, obtains a kind of new using sodium alginate and casein as compound wall materials
Possess the microcapsules of biocompatibility, which can be used for the bioactive substance for conveying a variety of sensitiveness.Optimize in invention
Wall material raw material and the addition of core loaded article have investigated core riboflavin and have existed to lift embedding rate of the microcapsules to core
Simulate the slow release effect in intestines, gastric juice.Microcapsules prepared by the present invention are compared with conventional sodium alginate composite micro-capsule, embedding rate
Higher, there is good sustained release performance under the conditions of stomach is simulated, and used casein can also discharge multiple functions after disassembly
Property polypeptide, and preparation process condition is gentle, operating condition is safe and simple, is conducive to mass produce.
Beneficial effects of the present invention
(1) casein provided by the invention/sodium alginate composite micro-capsule is compared with conventional sodium alginate composite micro-capsule,
Material therefor Nantural non-toxic, environmentally protective, preparation process safety and mild condition.
(2) casein produced by the present invention/sodium alginate micro-capsule surface is smooth, and the micro-capsule surface compact after freezing is smooth,
Tridimensional network, which is presented, without obvious slight crack and disrepair phenomenon, inside microballoon contributes to the release (see attached drawing 1-4) of core, with
Compared toward sodium alginate micro-capsule, effect of the micro-capsule in terms of active material cladding and sustained release is more preferable.
(3) microcapsules prepared by the present invention are compared with conventional micro-capsule, and core embedding rate is high, using riboflavin as model drug
Research in, the embedding rate of this casein/sodium alginate micro-capsule is up to 98%, and has under the conditions of stomach is simulated good slow
Performance is released, beneficial to abundant release (attached drawing 5,6) of the medicine under the conditions of intestines.
(4) special efficacy that the present invention possesses due to feature casein, compared with conventional microcapsules, can discharge a variety of
Active peptides, improve the immune and disease resistance (attached drawing 7) of body.
Brief description of the drawings
Fig. 1 is the photo figure in kind of casein/sodium alginate micro-capsule；
Fig. 2 is the electromicroscopic photograph figure of casein/sodium alginate micro-capsule entirety；
Fig. 3 is the electromicroscopic photograph figure on casein/sodium alginate micro-capsule surface；
Fig. 4 is the electromicroscopic photograph figure in casein/sodium alginate micro-capsule section；
Fig. 5 is casein/sodium alginate-elution profiles of the riboflavin micro-capsule in simulate the gastric juice；
Fig. 6 is casein/sodium alginate-elution profiles of the riboflavin micro-capsule in simulated intestinal fluid；
Fig. 7 is the reversed-phase high performance liquid chromatography figure of catabolite of the casein in stomach liquid is simulated；
Fig. 8 is CaCl2, core riboflavin and sodium alginate influence of the addition to microcapsule embedded rate.
Embodiment
With reference to the drawings and specific embodiments, the present invention is described in further detail：
The preparation of casein/sodium alginate composite microcapsule
1) casein solution is prepared first, then weighs sodium alginate powder, at room temperature mechanical agitation, is completely dissolved in it
Uniform casein/mixed solution of sodium alginate is formed in casein solution；
2) CaCl is prepared2Solution, at room temperature magnetic agitation obtain the solution of water white transparency as curing coagulating bath；
3) by above-mentioned casein/mixed solution of sodium alginate with 90 drop/point speed CaCl is added dropwise2It is solid in coagulating bath
Change, plural gel micro-capsule can be obtained.After curing 3-60min, micro-capsule is collected by filtration, and use distilled water flushing.
Wherein, the mass concentration of casein solution is 1-6% (%, w/v), and sodium alginate is mixed in casein/sodium alginate
The mass concentration closed in solution is 1.75-3.25% (%, w/v), CaCl2Mass concentration in its coagulating bath is 0.4-
0.8% (%, w/v).
The application of casein/sodium alginate composite microcapsule embedding molds medicine riboflavin core
Casein solution is prepared first, then weighs sodium alginate powder, at room temperature mechanical agitation, it is completely dissolved in junket
Uniform casein/mixed solution of sodium alginate is formed in protein solution.Riboflavin is weighed again and adds above-mentioned mixed solution, is formed
Casein/mixed solution of sodium alginate comprising riboflavin.CaCl is prepared again2Solution, at room temperature magnetic agitation obtain colourless
Bright solution is as curing coagulating bath.Wherein, the mass concentration of casein solution is 1-6% (%, w/v), sodium alginate soln
Mass concentration in casein/mixed solution of sodium alginate is 1.75-3.25% (%, w/v), CaCl2In its coagulating bath
Mass concentration be 0.4-0.8% (%, w/v), quality of the riboflavin in riboflavin/casein/mixed solution of sodium alginate
Concentration is 1.75-3.25mg/mL.CaCl is added dropwise in riboflavin/casein/mixed solution of sodium alginate2In coagulating bath, wink
Between can obtain plural gel micro-capsule.After curing 3-60min, micro-capsule is collected by filtration, and with distilled water flushing, obtained embedding molds medicine
The casein of riboflavin/sodium alginate micro gel capsule.
Preferably, the preparation process condition of microcapsules of the present invention is：Sodium alginate is mixed in casein/sodium alginate
The mass concentration closed in solution is 2.8% (w/v), CaCl2Mass concentration in its coagulating bath is 0.53% (w/v).
Preferably, the drugloading rate of model drug riboflavin is 3.25mg/ml in microcapsules of the present invention.
Embodiment 1
Casein is weighed, is dissolved at room temperature in distilled water, it is 8 to adjust pH value with the sodium hydrate aqueous solution of 1mol/L,
Magnetic agitation dissolving is made into the casein solution that mass concentration is 6% (%, w/v)；Sodium alginate is weighed, is dissolved in junket at room temperature
In protein solution, casein/mixed solution of sodium alginate is formed under magnetic stirring, and the mass concentration for making sodium alginate is
3.25% (%, w/v)；Riboflavin is weighed again and adds above-mentioned mixed solution, forms the medicine that riboflavin mass concentration is 3.25mg/mL
Thing-casein/mixed solution of sodium alginate.The CaCl that mass concentration is 0.8% (%, w/v) is prepared again2Solution, adjusts its pH
It is worth for 7, magnetic agitation obtains the solution of water white transparency as curing coagulating bath at room temperature.With the syringe with 5# syringe needles by core
Flavine-casein/mixed solution of sodium alginate injection CaCl2In solution, cure 60min after be collected by filtration, obtain casein/
Sodium alginate-riboflavin micro-capsule (as shown in Figure 8).Riboflavin content in filtrate is detected with ultraviolet spectrophotometry, and is calculated
Final casein/sodium alginate micro-capsule is 96.3% to the embedding rate of riboflavin.
Embodiment 2
Casein is weighed, is dissolved at room temperature in distilled water, it is 8 to adjust pH value with the sodium hydrate aqueous solution of 1mol/L,
Magnetic agitation dissolving is made into the casein solution that mass concentration is 1% (%, w/v)；Sodium alginate is weighed, is dissolved in junket at room temperature
In protein solution, casein/mixed solution of sodium alginate is formed under magnetic stirring, and the mass concentration for making sodium alginate is
1.75% (%, w/v)；Riboflavin is weighed again and adds above-mentioned mixed solution, forms the medicine that riboflavin mass concentration is 3.25mg/mL
Thing-casein/mixed solution of sodium alginate.The CaCl that mass concentration is 0.4% (%, w/v) is prepared again2Solution, adjusts its pH
It is worth for 7, magnetic agitation obtains the solution of water white transparency as curing coagulating bath at room temperature.With the syringe with 5# syringe needles by core
Flavine-casein/mixed solution of sodium alginate injection CaCl2In solution, cure 30min after be collected by filtration, obtain casein/
Sodium alginate-riboflavin micro-capsule (as shown in Figure 8).Riboflavin content in filtrate is detected with ultraviolet spectrophotometry, and is calculated
Final casein/sodium alginate micro-capsule is 91.8% to the embedding rate of riboflavin.
Embodiment 3
Casein is weighed, is dissolved at room temperature in distilled water, it is 8 to adjust pH value with the sodium hydrate aqueous solution of 1mol/L,
Magnetic agitation dissolving is made into the casein solution that mass concentration is 3% (%, w/v)；Sodium alginate is weighed, is dissolved in junket at room temperature
In protein solution, casein/mixed solution of sodium alginate is formed under magnetic stirring, and the mass concentration for making sodium alginate is
2.5% (%, w/v)；Riboflavin is weighed again and adds above-mentioned mixed solution, forms the medicine that riboflavin mass concentration is 3.25mg/mL
Thing-casein/mixed solution of sodium alginate.The CaCl that mass concentration is 0.6% (%, w/v) is prepared again2Solution, adjusts its pH
It is worth for 7, magnetic agitation obtains the solution of water white transparency as curing coagulating bath at room temperature.With the syringe with 5# syringe needles by core
Flavine-casein/mixed solution of sodium alginate injection CaCl2In solution, cure 30min after be collected by filtration, obtain casein/
Sodium alginate-riboflavin micro-capsule (as shown in Figure 8).Riboflavin content in filtrate is detected with ultraviolet spectrophotometry, and is calculated
Final casein/sodium alginate micro-capsule is 94.5% to the embedding rate of riboflavin.
Embodiment 4
Casein is weighed, is dissolved at room temperature in distilled water, it is 8 to adjust pH value with the sodium hydrate aqueous solution of 1mol/L,
Magnetic agitation dissolving is made into the casein solution that mass concentration is 1% (%, w/v)；Sodium alginate is weighed, is dissolved in junket at room temperature
In protein solution, casein/mixed solution of sodium alginate is formed under magnetic stirring, and the mass concentration for making sodium alginate is
2.8% (%, w/v)；Riboflavin is weighed again and adds above-mentioned mixed solution, forms the medicine that riboflavin mass concentration is 1.75mg/mL
Thing-casein/mixed solution of sodium alginate.The CaCl that mass concentration is 0.4% (%, w/v) is prepared again2Solution, adjusts its pH
It is worth for 7, magnetic agitation obtains the solution of water white transparency as curing coagulating bath at room temperature.With the syringe with 5# syringe needles by core
Flavine-casein/mixed solution of sodium alginate injection CaCl2In solution, cure 30min after be collected by filtration, obtain casein/
Sodium alginate-riboflavin micro-capsule (as shown in Figure 8).Riboflavin content in filtrate is detected with ultraviolet spectrophotometry, and is calculated
Final casein/sodium alginate micro-capsule is 90.1% to the embedding rate of riboflavin.
Embodiment 5
Casein is weighed, is dissolved at room temperature in distilled water, it is 8 to adjust pH value with the sodium hydrate aqueous solution of 1mol/L,
Magnetic agitation dissolving is made into the casein solution that mass concentration is 1% (%, w/v)；Sodium alginate is weighed, is dissolved in junket at room temperature
In protein solution, casein/mixed solution of sodium alginate is formed under magnetic stirring, and the mass concentration for making sodium alginate is
2.8% (%, w/v)；Riboflavin is weighed again and adds above-mentioned mixed solution, forms the medicine that riboflavin mass concentration is 2.5mg/mL
Thing-casein/mixed solution of sodium alginate.The CaCl that mass concentration is 0.4% (%, w/v) is prepared again2Solution, adjusts its pH
It is worth for 7, magnetic agitation obtains the solution of water white transparency as curing coagulating bath at room temperature.With the syringe with 5# syringe needles by core
Flavine-casein/mixed solution of sodium alginate injection CaCl2In solution, cure 30min after be collected by filtration, obtain casein/
Sodium alginate-riboflavin micro-capsule (as shown in Figure 8).Riboflavin content in filtrate is detected with ultraviolet spectrophotometry, and is calculated
Final casein/sodium alginate micro-capsule is 94.4% to the embedding rate of riboflavin.
Embodiment 6
Casein is weighed, is dissolved at room temperature in distilled water, it is 8 to adjust pH value with the sodium hydrate aqueous solution of 1mol/L,
Magnetic agitation dissolving is made into the casein solution that mass concentration is 1% (%, w/v)；Sodium alginate is weighed, is dissolved in junket at room temperature
In protein solution, casein/mixed solution of sodium alginate is formed under magnetic stirring, and the mass concentration for making sodium alginate is
1.75% (%, w/v)；Riboflavin is weighed again and adds above-mentioned mixed solution, forms the medicine that riboflavin mass concentration is 1.75mg/mL
Thing-casein/mixed solution of sodium alginate.The CaCl that mass concentration is 0.4% (%, w/v) is prepared again2Solution, adjusts its pH
It is worth for 7, magnetic agitation obtains the solution of water white transparency as curing coagulating bath at room temperature.With the syringe with 5# syringe needles by core
Flavine-casein/mixed solution of sodium alginate injection CaCl2In solution, it is collected by filtration after curing 3min, obtains casein/sea
Mosanom-riboflavin micro-capsule (as shown in Figure 8).Riboflavin content in filtrate is detected with ultraviolet spectrophotometry, and is calculated most
Whole casein/sodium alginate micro-capsule is 88.5% to the embedding rate of riboflavin.
Claims (7)
1. a kind of preparation method of casein/sodium alginate composite microcapsule, it is characterized in that：
1) casein solution is prepared, then weighs sodium alginate powder, mechanical agitation, makes it be completely dissolved in casein molten at room temperature
Uniform casein/mixed solution of sodium alginate is formed in liquid；
2) CaCl is prepared2Solution, at room temperature magnetic agitation obtain the solution of water white transparency as curing coagulating bath；
3) above-mentioned casein/mixed solution of sodium alginate is added dropwise to CaCl2Cure in coagulating bath, obtain plural gel micro-capsule；Cure
After 3-60min, micro-capsule is collected by filtration, and use distilled water flushing.
2. the method as described in claim 1, it is characterized in that the mass concentration of casein solution is 1-6% (%, w/v), seaweed
Mass concentration of the sour sodium in casein/mixed solution of sodium alginate is 1.75~3.25% (%, w/v), CaCl2In its solidification
Mass concentration in bath is 0.4-0.8% (%, w/v).
3. the method as described in claim 1, it is characterized in that sodium alginate exists in the casein/sodium alginate composite microcapsule
Mass concentration in casein/mixed solution of sodium alginate is 2.8% (w/v), CaCl2Mass concentration in its coagulating bath is
0.53% (w/v).
4. the method as described in claim 1, it is characterized in that the rate of addition of step 3) be with 90 drops/point.
5. the application of casein/sodium alginate composite microcapsule embedding molds medicine riboflavin core prepared by claim 1, it is special
Sign is：Casein solution is prepared first, then weighs sodium alginate powder, at room temperature mechanical agitation, it is completely dissolved in junket egg
Uniform casein/mixed solution of sodium alginate is formed in white solution；Riboflavin is weighed again and adds above-mentioned mixed solution, forms bag
Casein/mixed solution of sodium alginate containing riboflavin；CaCl is prepared again2Solution, at room temperature magnetic agitation obtain water white transparency
Solution as cure coagulating bath.
6. application as claimed in claim 5, it is characterized in that riboflavin/casein/mixed solution of sodium alginate is added dropwise to
CaCl2In coagulating bath, plural gel micro-capsule is obtained；After curing 3-60min, micro-capsule is collected by filtration, and with distilled water flushing, be made and wrap
Bury casein/sodium alginate micro gel capsule of model drug riboflavin.
7. application as claimed in claim 5, it is characterized in that the drugloading rate of model drug riboflavin is in the microcapsules
3.25mg/ml。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711065031.5A CN107970227A (en) | 2017-11-02 | 2017-11-02 | A kind of preparation method of casein/sodium alginate composite microcapsule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711065031.5A CN107970227A (en) | 2017-11-02 | 2017-11-02 | A kind of preparation method of casein/sodium alginate composite microcapsule |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107970227A true CN107970227A (en) | 2018-05-01 |
Family
ID=62012932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711065031.5A Pending CN107970227A (en) | 2017-11-02 | 2017-11-02 | A kind of preparation method of casein/sodium alginate composite microcapsule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107970227A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110859306A (en) * | 2019-12-03 | 2020-03-06 | 东北农业大学 | Preparation method of hydrogel beads |
| CN111228239A (en) * | 2020-01-13 | 2020-06-05 | 厦门金达威生物科技有限公司 | PH sensitive type gel microcapsule and preparation method thereof |
| CN111450077A (en) * | 2020-03-12 | 2020-07-28 | 上海海洋大学 | Millimeter-sized multinuclear capsule for delivering nutrient substances/medicines to small intestine and slowly releasing nutrients/medicines, and preparation method and application thereof |
| CN111466575A (en) * | 2020-04-22 | 2020-07-31 | 吉林农业大学 | Preparation method of functional composite protein emulsion gel |
| CN111514097A (en) * | 2020-05-14 | 2020-08-11 | 燕山大学 | Preparation method of pH-responsive nano hydrogel of walnut shell polyphenol |
| CN111617052A (en) * | 2020-05-30 | 2020-09-04 | 泉州玺阅日化有限公司 | Seal oil microcapsule and preparation method thereof |
| CN111905106A (en) * | 2020-09-14 | 2020-11-10 | 陕西科技大学 | Slow-release sodium alginate/chitosan/zein composite material and preparation method thereof |
| CN112048080A (en) * | 2020-09-14 | 2020-12-08 | 陕西科技大学 | Microcapsule-filled sodium alginate-based hydrogel and preparation method thereof |
| CN114080212A (en) * | 2019-07-09 | 2022-02-22 | 株式会社Lg生活健康 | Cosmetic capsule |
| CN114437371A (en) * | 2022-01-14 | 2022-05-06 | 陕西科技大学 | Preparation method of casein-based nano hybrid hydrogel based on double cross-linking mechanism |
| CN116099467A (en) * | 2023-02-21 | 2023-05-12 | 吉林大学 | Quercetin microcapsule and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070048295A1 (en) * | 2005-08-23 | 2007-03-01 | Ming-Ju Chen | Method for preparing alginate capsules |
| CN107254181A (en) * | 2017-06-05 | 2017-10-17 | 江苏大学 | The application of the supersonically preparation method and functional food of casein polysaccharide nanogel |
-
2017
- 2017-11-02 CN CN201711065031.5A patent/CN107970227A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070048295A1 (en) * | 2005-08-23 | 2007-03-01 | Ming-Ju Chen | Method for preparing alginate capsules |
| CN107254181A (en) * | 2017-06-05 | 2017-10-17 | 江苏大学 | The application of the supersonically preparation method and functional food of casein polysaccharide nanogel |
Non-Patent Citations (1)
| Title |
|---|
| HE ZHIMIN ET AL.: "Alginate-Casein Microspheres as Bioactive Vehicles for Nutrients", 《TRANSACTIONS OF TIANJING UNIVERSITY》 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114080212A (en) * | 2019-07-09 | 2022-02-22 | 株式会社Lg生活健康 | Cosmetic capsule |
| CN110859306A (en) * | 2019-12-03 | 2020-03-06 | 东北农业大学 | Preparation method of hydrogel beads |
| CN111228239A (en) * | 2020-01-13 | 2020-06-05 | 厦门金达威生物科技有限公司 | PH sensitive type gel microcapsule and preparation method thereof |
| CN111228239B (en) * | 2020-01-13 | 2022-04-22 | 厦门金达威生物科技有限公司 | PH sensitive type gel microcapsule and preparation method thereof |
| CN111450077A (en) * | 2020-03-12 | 2020-07-28 | 上海海洋大学 | Millimeter-sized multinuclear capsule for delivering nutrient substances/medicines to small intestine and slowly releasing nutrients/medicines, and preparation method and application thereof |
| CN111450077B (en) * | 2020-03-12 | 2022-07-05 | 上海海洋大学 | Millimeter-sized multinuclear capsule for delivering nutrient substances/medicines to small intestine and slowly releasing nutrients/medicines, and preparation method and application thereof |
| CN111466575A (en) * | 2020-04-22 | 2020-07-31 | 吉林农业大学 | Preparation method of functional composite protein emulsion gel |
| CN111514097A (en) * | 2020-05-14 | 2020-08-11 | 燕山大学 | Preparation method of pH-responsive nano hydrogel of walnut shell polyphenol |
| CN111617052A (en) * | 2020-05-30 | 2020-09-04 | 泉州玺阅日化有限公司 | Seal oil microcapsule and preparation method thereof |
| CN112048080A (en) * | 2020-09-14 | 2020-12-08 | 陕西科技大学 | Microcapsule-filled sodium alginate-based hydrogel and preparation method thereof |
| CN111905106A (en) * | 2020-09-14 | 2020-11-10 | 陕西科技大学 | Slow-release sodium alginate/chitosan/zein composite material and preparation method thereof |
| CN112048080B (en) * | 2020-09-14 | 2022-11-08 | 陕西科技大学 | Microcapsule-filled sodium alginate-based hydrogel and preparation method thereof |
| CN114437371A (en) * | 2022-01-14 | 2022-05-06 | 陕西科技大学 | Preparation method of casein-based nano hybrid hydrogel based on double cross-linking mechanism |
| CN114437371B (en) * | 2022-01-14 | 2023-09-05 | 陕西科技大学 | Preparation method of casein-based nano hybrid hydrogel based on double crosslinking mechanism |
| CN116099467A (en) * | 2023-02-21 | 2023-05-12 | 吉林大学 | Quercetin microcapsule and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107970227A (en) | A kind of preparation method of casein/sodium alginate composite microcapsule | |
| Li et al. | Alginate‐based delivery systems for food bioactive ingredients: An overview of recent advances and future trends | |
| Lim et al. | Controlled delivery of oral insulin aspart using pH-responsive alginate/κ-carrageenan composite hydrogel beads | |
| Goh et al. | Alginates as a useful natural polymer for microencapsulation and therapeutic applications | |
| Patil et al. | A review on ionotropic gelation method: novel approach for controlled gastroretentive gelispheres | |
| Cikrikci et al. | Development of pH sensitive alginate/gum tragacanth based hydrogels for oral insulin delivery | |
| Wang et al. | Microencapsulation using natural polysaccharides for drug delivery and cell implantation | |
| Javanbakht et al. | Facile preparation of pH-responsive k-Carrageenan/tramadol loaded UiO-66 bio-nanocomposite hydrogel beads as a nontoxic oral delivery vehicle | |
| Zhao et al. | Dual-core prebiotic microcapsule encapsulating probiotics for metabolic syndrome | |
| Li et al. | Improvement of fucoxanthin oral efficacy via vehicles based on gum Arabic, gelatin and alginate hydrogel: Delivery system for oral efficacy enhancement of functional food ingredients | |
| CN105661543B (en) | A kind of stable type intestines sustained release tea polyphenols microcapsules and preparation method thereof | |
| CN102552546A (en) | Tea polyphenol sodium alginate microsphere and preparation method and application thereof | |
| Wang et al. | Electrosprayed soft capsules of millimeter size for specifically delivering fish oil/nutrients to the stomach and intestines | |
| Liu et al. | Fabrication of fish gelatin/sodium alginate double network gels for encapsulation of probiotics | |
| KR101722476B1 (en) | A microencapsulation process of sertoli cells, microcapsules obtained and their use for prevention and cure of type 1 diabetes mellitus | |
| Yadav et al. | Polysaccharide-silicate composite hydrogels: Review on synthesis and drug delivery credentials | |
| CN107970228A (en) | A kind of preparation method using chitosan-TPP-KGM as the nano-microcapsule of compound wall materials | |
| US20230190665A1 (en) | Therapeutic protein-loaded nanoparticle and method for preparing the same | |
| Tao et al. | A review on the chemical modification of alginates for food research: Chemical nature, modification methods, product types, and application | |
| CN1241552C (en) | Konjaku gluocomannan-calcium alginate microball for carrying medicine and preparing method | |
| Aw et al. | Pickering emulsion hydrogel beads for curcumin encapsulation and food application | |
| Zhu et al. | Encapsulation of SOD in chitosan-coated gel particles of alginate or mixture of alginate and shellac for targeted intestinal delivery | |
| Xu et al. | Controlled release of Lactiplantibacillus plantarum by colon-targeted adhesive pectin microspheres: Effects of pectin methyl esterification degrees | |
| Fathi et al. | Natural hydrogels, the interesting carriers for herbal extracts | |
| Singha et al. | Applications of alginate-based bionanocomposites in drug delivery |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180501 |
|
| RJ01 | Rejection of invention patent application after publication |