CN1079606A - Packaging method for making human-body specimen - Google Patents
Packaging method for making human-body specimen Download PDFInfo
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- CN1079606A CN1079606A CN 93106329 CN93106329A CN1079606A CN 1079606 A CN1079606 A CN 1079606A CN 93106329 CN93106329 CN 93106329 CN 93106329 A CN93106329 A CN 93106329A CN 1079606 A CN1079606 A CN 1079606A
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Abstract
The present invention relates to a kind of packaging method for making human-body specimen.This method is exactly that anticorrosion fixing sample envelope is embedded in the residuite that condensable forms, and sample is hedged off from the outer world, thereby reaches the purpose of long preservation.This method has been exempted the burden of using specimen bottle and preserving liquid in the wet preservation method, can prevent the murder by poisoning of formaldehyde pernicious gas to human body.Its good seal performance, easy to use, all there is important use to be worth to the exploitation of teaching and scientific research and handicraft.
Description
The present invention relates to a kind of packaging method for making human-body specimen, specifically anticorrosion fixing human body specimen envelope is embedded in the transparent matrix that condensable forms, sample is hedged off from the outer world, thereby reaches the purpose of long preservation human body specimen.The envelope that it is widely used in various biologies and herbarium is buried and the making of handicraft, and teaching and medical research are all had very important use value.
Current store method to the soft sample of human body is also very backward, both uneconomical, and also science not at home and abroad also all is to adopt traditional moist preservation method, i.e. impregnating fluid infusion method.Soft sample is put into vial or lucite container, and the preparation formalin soaks, and not only places occupations of land side, and the easy polymerization of formalin and precipitating, and needs constantly replacing liquid, has the formaldehyde pernicious gas unavoidably to spill the murder by poisoning human body.China for a long time just the someone want to improve the store method of the soft sample of human body, but all do not have successfully.Enter the eighties, the anatomy expert of indivedual countries is arranged in West Europe, once buried human body specimen with the plastics envelope, and obtained progress, but because technology is simple, the sample profile that envelope is buried is very not attractive in appearance, the sample shrinkage is obvious, poor transparency, and actual use value is little.
Purpose of the present invention is exactly that a kind of new method that adopts the resin envelope to bury human body specimen is provided in order to overcome weak point of the prior art, it can make the sample holding time long, transparency good, teaching, view and admire more directly perceived, higher to the diagnosis of CT and operation, clinical medicine, medical research using value especially.
Purpose of the present invention can reach by following measure:
One, the preparation of sample: want the conscientious preservative treatment of carrying out with sealing the sample that buries, shave then except that hair, pubes, whole body and carry out preserved skin, again with washing powder, refer to that brush scrubs repeatedly, clear water washes down, airing, stamps markings by the desire section.
Two, the freezing and saw blade of sample: cadaver sample is freezing in-30～40 ℃ environment, freeze " thoroughly " after, be firmly fixed on the workbench freezing good corpse, saw down piecewise by the fixed signal line, after both sides of plane part is smooth, number consecutively.
Three, the flushing of sample and pruning: after sample is sawn into sheet, cross-section specimen is soaked in the square plate of filling running water piecewise, naturally thaw, with hairbrush Dissolve things inside in the enteric cavity and intravascular coagulation piece are cleared up, scrub clean, and then be placed on this face with the bottom surface of another square plate and overturn, in the same way another side is also scrubbed clean.Repeated multiple times like this, till the clear thoroughly no-sundries of water, the water floating method is all wiped out the fine hair shape floating thing on sample two sides.
Four, sample dehydration: because resin is very little with the mutual solubility of water, so and mist-like bleary can take place after water contacted, do not see after sample is imbedded, when the envelope of wet sample is buried, should note the dehydration of sample, and method after dewatering and time to be noted grasp; Its method is as follows:
(1) dehydration is that sample is put into 45～55% alcohol for the first time, and adds 8～15% glacial acetic acid, and sample is 40～50 hours resting periods in alcohol.
(2) dehydration for the second time be with sample from last liquid, takes out afterwards blot with blotting paper after, be put into again in 55～65% alcohol, ice acetic acid 8～15% again, park after 40～50 hours and take out sample.
(3) dehydration is after sample is taken out from last liquid for the third time, after blotting with blotting paper, sample is put into 65～75% alcohol again, adds glacial acetic acid 8～15% again, parks 40～50 hours.
(4) the 4th dehydrations are after sample is taken out from 65～75% alcohol, after blotting with blotting paper, sample are put into 80%～90%～100%～100% alcohol again, cross twice in 100% alcohol.
(5) treat above dehydration several times after, after sample taken out from 100% alcohol, airing, with blotting paper with the alcohol on surface blot clean after, shaping, prune and labelled (or explanation) after stand-by.
Five, the making of mould:
Mould design, to make according to size and form, the thickness of sample, the material of mould can adopt galvanized iron sheet, glass and steel plate to make, then mould is placed on the glass plate, post with adhesive plaster or paper all around, prevent that resin from leaking outside, after drying, with brush one deck release agent in the mould, prevent demoulding difficulty.
Six, envelope is buried:
According to the size of mould, determine the resin aequum.
(1) an amount of resin is poured in the beaker, after stirring with glass bar, added that 0.5～2% initator is proceeded to stir, and then add promoter 0.5～6% and stir.
(2) mixed resin is poured in the mould felt secure, treat that the end puts into sample after condensing, pour second layer resin again into, generally divide 3～5 layers of perfusion to be advisable.
Room temperature must remain on 20～40 ℃ when (3) envelope was buried.
Envelope is buried used primary raw material-resin, is a kind of unsaturated polyester resin.This unsaturated polyester resin can be done promoter gel and curing under room temperature with the methyl ethyl ketone peroxide cobalt.
Seven, the demoulding and polishing:
After polymerization is finished, take out polymeric block, the envelope of taking out is buried piece polish, till polishing flat according to the form of design.The envelope that to tie is buried piece with waterproof abrasive paper water mill repeatedly from coarse to fine then, is ground to till the no line always.
Eight, polishing:
To polish with the polishing wheel that calico is made behind the water mill, will use antiscuffing paste on the cloth wheel, polish repeatedly, throwing as the natural water spar bright till.
Nine, dress box:
According to the size and form of sample, thickness designs the making of brocade box.After box makes, sample is put into box get final product.
The present invention has following advantage compared to existing technology:
1, this method has been exempted the burden of using specimen bottle and preserving liquid in the wet preservation method, and can prevent the murder by poisoning of formaldehyde pernicious gas to human body.
2, can condense in human body specimen in the residuite, sample is hedged off from the outer world, thereby reach the purpose of long preservation.
3, the sample that adopts this method to make, transparency is strong, and good seal performance is easy to use, and teaching and scientific research are all had very significant values.
4, this method envelope that can also be applied to various biologies and herbarium is buried and the making of handicraft, has a extensive future.
The present invention is described in further detail relevant situation below in conjunction with embodiment: will keep a close eye on the variations in temperature that envelope is buried sample when envelope is buried, if will reduce promoter when temperature is high, can also take cooling measure, prevent implode, can strengthen the consumption of promoter as temperature is low.About the polymerization speed problem, also be the key issue that envelope is buried sample.So when envelope is buried, will note suitable polymerization speed.Speed is too slow, and the sample internal moisture can be oozed out, and makes the sample surface occur vaporific and muddy; Speed is too fast, can produce a large amount of heat of polymerizations in a short time, is difficult in time come out, and implode easily takes place.The speed of polymerisation is subjected to the influence of factors such as temperature, pressure initator, and human body specimen envelope is buried, and can only carry out to good under 20～40 ℃ of room temperatures.Therefore, the height of temperature is selected on the initator condition.Select the lower initator of decomposition temperature, and note grasping initator, the temperature requirement of consumption and polymerization process.
Simultaneously, the preparation of apparatus and envelope are buried method and requirement has substantial connection.Therefore, in order to avoid sealing, influence bury effect according to the operation sequence work of getting ready carefully before the work.Here introduce a part equipment, medicine and purposes commonly used.
One, training equipment commonly used:
(1) abrasive machine: use during polishing
(2) polishing machine: use during the polishing sample
(3) 1, beaker; 2, amount mark; 3, graduated cylinder; 4, glass bar; 5, scalpel; 6, operating scissors; 7, blotting paper; 8, waterproof abrasive paper 350～400#; 9, gauze; 10, glass cutter; 11, mould; Available steel also can be made of galvanized iron sheet or glass; 12, thermometer; 13, alcohol protractor; 14, sample dehydration tube; 15, workbench.
Two, common drug:
(1) resin: being that envelope is buried used primary raw material, is a kind of unsaturated polyester resin, also is a kind of middle active, moderately viscous adjacent benzene type casting resin.This unsaturated polyester resin can be made initator with methyl ethyl ketone peroxide or liquid peroxy ring ethyl ketone, and cobalt naphthenate does to promote gel and curing under room temperature.Look shallow is transparent, hardening process is milder, good processability, 25 ℃ of PZS0.8～1.3 of viscosity, 25 ℃ of MM5～10 of gel time, acid number MGKOH/17～25, solids content %65～.71,80 ℃ of N time＞24 of heat endurance, physical property casting matrix, Ba Ke hardness＞30, impact strength KJ/M2 〉=7, bending strength NTP80, tensile strength NTPQ50, condition of cure: 4 parts of 100 parts of initators of resin " L ", condition of cure after promoter E0.5 part, 80 ℃ were dried by the fire 8 hours, and the shady and cool ventilation place is left in the sealing of this medicine storage request in, isolated kindling material thermal source, the rotten and moisture content infiltration with volatilization prevention burning polymerization.
(2) initator: mainly be the effect that causes resin solidification.
(3) promoter: mainly be to promote the resin solidification effect.
(4) alcohol: mainly be that the dehydration processing of sample is used.
(5) glacial acetic acid: mainly be to prevent that sample distortion from dwindling.
Claims (5)
1, a kind ofly can be embedded in packaging method for making human-body specimen in the residuite that condensable forms to anticorrosion fixing human body specimen envelope, it is characterized in that sealing and bury used resin, being a kind of unsaturated polyester resin, also is a kind of middle active, moderately viscous adjacent benzene type casting resin.
2, bury method according to the described human body specimen of claim envelope, it is characterized in that this unsaturated polyester resin can make initator with methyl ethyl ketone peroxide or liquid peroxy ring ethyl ketone, cobalt naphthenate is done promoter.
3, a kind of packaging method for making human-body specimen is characterized in that the dewatering and the time of sample, the specification requirement that envelope is buried.
4, packaging method for making human-body specimen according to claim 3 is characterized in that:
(1) dehydration is that sample is put into 45～55% alcohol for the first time, adds 8～15% glacial acetic acid, and sample was deposited in alcohol 40～50 hours.
(2) dehydration for the second time is after sample is blotted with blotting paper with (1) middle taking-up, to be put in 55～65% alcohol again, adds glacial acetic acid 8～15%, parks 40～50 hours.
(3) dehydration is that sample is taken out from (2) for the third time, after blotting with blotting paper, puts into 65～75% alcohol again, adds glacial acetic acid 8～15%, parks 40～50 hours.
(4) the 4th dehydrations are that sample is taken out from (3), after blotting with blotting paper, put into 80%～90%～100%～100% alcohol again, cross twice in 100% alcohol.
(5) sample is taken out back, airing from 100% alcohol, after with blotting paper the alcohol on surface being blotted, shaping, prune and labelled back stand-by.
5, according to claim 1,2,3 described packaging method for making human-body specimen, it is characterized in that: room temperature must remain on 20～40 ℃ when (1) envelope was buried.(2) an amount of resin is poured in the beaker, after stirring with glass bar, added 0.5～2% initator and proceed to stir, and then add promoter 0.5～6% and stir.(3) resin that mixes poured in the mould felt secure, treat that the end condenses after, put into sample, pour second layer resin again into, generally divide 3～5 layers of perfusion to be advisable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93106329 CN1079606A (en) | 1993-05-28 | 1993-05-28 | Packaging method for making human-body specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93106329 CN1079606A (en) | 1993-05-28 | 1993-05-28 | Packaging method for making human-body specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1079606A true CN1079606A (en) | 1993-12-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93106329 Pending CN1079606A (en) | 1993-05-28 | 1993-05-28 | Packaging method for making human-body specimen |
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| CN (1) | CN1079606A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060012C (en) * | 1994-09-23 | 2001-01-03 | 段永福 | Plexiglass specimen embedding method and its formula |
| CN102504510A (en) * | 2011-10-14 | 2012-06-20 | 大连鸿峰生物科技有限公司 | Embedding agent for biologic tissue plasticized slice |
| CN103953218A (en) * | 2014-03-05 | 2014-07-30 | 罗能全 | Environment-friendly hanging coffin public cemetery |
| CN107063810A (en) * | 2016-12-09 | 2017-08-18 | 中国科学院海洋研究所 | The novel method of marine organism specimen is preserved using epoxy resin |
| CN107727479A (en) * | 2017-09-30 | 2018-02-23 | 福建中医药大学 | A kind of method that cast moulds embedding preserves |
| CN109049460A (en) * | 2018-07-09 | 2018-12-21 | 张家港市德仁科教仪器设备有限公司 | A kind of liver cast sample embedding method and model |
| CN109049459A (en) * | 2018-07-09 | 2018-12-21 | 张家港市德仁科教仪器设备有限公司 | A kind of foot perivascular nerves sample embedding method and model |
| CN113812397A (en) * | 2021-09-30 | 2021-12-21 | 佛山市第一人民医院（中山大学附属佛山医院） | Perfusion preservation liquid |
-
1993
- 1993-05-28 CN CN 93106329 patent/CN1079606A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060012C (en) * | 1994-09-23 | 2001-01-03 | 段永福 | Plexiglass specimen embedding method and its formula |
| CN102504510A (en) * | 2011-10-14 | 2012-06-20 | 大连鸿峰生物科技有限公司 | Embedding agent for biologic tissue plasticized slice |
| CN103953218A (en) * | 2014-03-05 | 2014-07-30 | 罗能全 | Environment-friendly hanging coffin public cemetery |
| CN107063810A (en) * | 2016-12-09 | 2017-08-18 | 中国科学院海洋研究所 | The novel method of marine organism specimen is preserved using epoxy resin |
| CN107727479A (en) * | 2017-09-30 | 2018-02-23 | 福建中医药大学 | A kind of method that cast moulds embedding preserves |
| CN109049460A (en) * | 2018-07-09 | 2018-12-21 | 张家港市德仁科教仪器设备有限公司 | A kind of liver cast sample embedding method and model |
| CN109049459A (en) * | 2018-07-09 | 2018-12-21 | 张家港市德仁科教仪器设备有限公司 | A kind of foot perivascular nerves sample embedding method and model |
| CN113812397A (en) * | 2021-09-30 | 2021-12-21 | 佛山市第一人民医院（中山大学附属佛山医院） | Perfusion preservation liquid |
| CN113812397B (en) * | 2021-09-30 | 2023-03-14 | 佛山市第一人民医院（中山大学附属佛山医院） | Perfusion preserving fluid |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |