CN107960463A - A kind of acidified milk rich in animal bifidobacteria and preparation method thereof - Google Patents
A kind of acidified milk rich in animal bifidobacteria and preparation method thereof Download PDFInfo
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- CN107960463A CN107960463A CN201711434666.8A CN201711434666A CN107960463A CN 107960463 A CN107960463 A CN 107960463A CN 201711434666 A CN201711434666 A CN 201711434666A CN 107960463 A CN107960463 A CN 107960463A
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- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 88
- 235000020167 acidified milk Nutrition 0.000 title claims abstract description 83
- 241001465754 Metazoa Species 0.000 title claims abstract description 80
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 241000192130 Leuconostoc mesenteroides Species 0.000 claims abstract description 40
- 238000001816 cooling Methods 0.000 claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 28
- 230000004151 fermentation Effects 0.000 claims abstract description 28
- 210000000481 breast Anatomy 0.000 claims abstract description 27
- 235000020185 raw untreated milk Nutrition 0.000 claims abstract description 24
- 238000011049 filling Methods 0.000 claims abstract description 10
- 238000012856 packing Methods 0.000 claims abstract description 10
- 238000005057 refrigeration Methods 0.000 claims abstract description 4
- 235000013336 milk Nutrition 0.000 claims description 18
- 239000008267 milk Substances 0.000 claims description 18
- 210000004080 milk Anatomy 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 229930006000 Sucrose Natural products 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 239000005720 sucrose Substances 0.000 claims description 13
- 239000002054 inoculum Substances 0.000 claims description 8
- 235000014012 manganese gluconate Nutrition 0.000 claims description 7
- 235000008476 powdered milk Nutrition 0.000 claims description 7
- 235000020183 skimmed milk Nutrition 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 241001134770 Bifidobacterium animalis Species 0.000 claims description 5
- 229940118852 bifidobacterium animalis Drugs 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 6
- 230000000968 intestinal effect Effects 0.000 abstract description 4
- 235000013406 prebiotics Nutrition 0.000 abstract description 3
- 210000000936 intestine Anatomy 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 240000006024 Lactobacillus plantarum Species 0.000 abstract 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 abstract 1
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- 239000002994 raw material Substances 0.000 description 13
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- 241000894006 Bacteria Species 0.000 description 10
- 230000036541 health Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
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- 239000012895 dilution Substances 0.000 description 6
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- 235000013305 food Nutrition 0.000 description 5
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- 229940039696 lactobacillus Drugs 0.000 description 2
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
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- 241000196324 Embryophyta Species 0.000 description 1
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- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
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- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
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- 238000003501 co-culture Methods 0.000 description 1
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- 230000037213 diet Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
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- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 235000021472 generally recognized as safe Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
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- 230000006872 improvement Effects 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940118199 levulan Drugs 0.000 description 1
- 229940072543 manganese gluconate Drugs 0.000 description 1
- 239000011683 manganese gluconate Substances 0.000 description 1
- OXHQNTSSPHKCPB-IYEMJOQQSA-L manganese(2+);(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Mn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O OXHQNTSSPHKCPB-IYEMJOQQSA-L 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1236—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using Leuconostoc, Pediococcus or Streptococcus sp. other than Streptococcus Thermophilus; Artificial sour buttermilk in general
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/31—Leuconostoc
- A23V2400/321—Mesenteroides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
Abstract
The invention discloses a kind of preparation method of the acidified milk rich in animal bifidobacteria, its preparation method comprises the following steps：(1) animal bifidobacteria and Leuconostoc mesenteroides BD01710 are inoculated with into raw milk respectively, is uniformly mixed, at 30 37 DEG C of temperature, it is 70 95 ° of T to be left to ferment to titratable acidity, obtains acidified milk；(2) acidified milk made from step (1) is cooled to 16 22 DEG C, obtains cooling and fermentation breast；(3) it is cooling and fermentation breast is filling into packing container, then it is transferred in the environment that temperature is 2 10 DEG C and carries out 12 24h of refrigeration after-ripening, you can the acidified milk of animal bifidobacteria must be rich in.Acidified milk rich in lactobacillus plantarum prepared by the present invention is full of nutrition, in good taste, preparation method is simple and practicable, competent animals Bifidobacterium content in acidified milk is high, it can effectively play after consumption and adjust the prebiotic function such as intestinal flora balance and the effect of whole intestines, there is great market application potential and economic value.
Description
Technical field
The present invention relates to technical field of microbial fermentation, and in particular to a kind of acidified milk rich in animal bifidobacteria and its
Preparation method.
Background technology
Streptococcus thermophilus (Streptococcus thermophilus)-lactobacillus bulgaricus (Lactobacillus
Bulgaricus) combination is leavening combination most classical in current acidified milk.But both lactic acid bacterias are to digestive tract environment
Tolerance is limited, is not preferable probiotics.Therefore, industrial circle is attempting acidified milk body of the exploitation based on probiotics always
System.
Probiotics (Probiotics) is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, reproduction
In system, definite health efficacy can be produced so as to the active beneficial microbe for improving host's microecological balance, playing beneficial effect
General name.Mainly there is common probiotics in human body, animal body：Clostridium butyricum, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus etc..
Bifidobacterium is that play an important roll a kind of lactic acid bacteria to the health of enteron aisle and organism level.Research shows, Bifidobacterium conduct
A kind of physiological beneficial bacterium, has biological barrier, trophism, antitumor action, immunological enhancement, improvement to health
Gastrointestinal function, anti-ageing a variety of important physiological functions of waiting for a long time.And person in middle and old age's stage is arrived, the quantity of Bifidobacterium starts to decay,
At the same time the harmful bacteria such as clostridium is bred, this can adversely affect gastrointestinal health.Moreover, the use of diet, pressure, medicine
It is unbalance that intestinal flora is also resulted in the change of environment etc..Therefore, Bifidobacterium is supplemented by the approach of food, had to human body
Important nutrient health and Disease Intervention effect.
However, Bifidobacterium is also faced with a main technical bottleneck in field of dairy products scale application, have now been found that
Bifidobacterium strain be difficult to grow in newborn medium.For this reason, constantly there is research to attempt fermentation body of the exploitation based on probiotics
System, such as promotes the confactor of growth, and Lactobacillus rhamnosus and streptococcus thermophilus group by being added in fermentation system
Close, Lactococcus lactis and the combination of gossypose lactobacillus etc..Although the studies above has some technological break-throughs, but generally pass through to
Extra, promotion growth component is added in newborn medium, has significant limitation in theoretical research and production application.
The content of the invention
The purpose of the present invention is for animal bifidobacteria (Bifidobacterium animalis) in breast hardly
The technical barrier such as low with production slow acid, strain number content is grown, Leuconostoc mesenteroides (Leuconostoc is found by experiment
Mesenteroides) BD01710 provides a kind of rich in dynamic the growth-promoting effect of animal bifidobacteria on this basis
The acidified milk of thing Bifidobacterium, and the preparation method of the acidified milk.
The present invention's is to reach above-mentioned purpose, is achieved especially by following technical scheme：
A kind of preparation method of the acidified milk rich in animal bifidobacteria, its preparation method comprise the following steps：(1) respectively
Animal bifidobacteria (Bifidobacterium animalis) and Leuconostoc mesenteroides are inoculated with into raw milk
(Leuconostoc mesenteroides) BD01710, is uniformly mixed, at 30-37 DEG C of temperature, standing for fermentation to drop
It is 70-95 ° of T to determine acidity, obtains acidified milk；(2) acidified milk made from step (1) is cooled to 16-22 DEG C, obtains cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred in the environment that temperature is 2-10 DEG C and carries out refrigeration after-ripening 12-
24h, you can the acidified milk of animal bifidobacteria must be rich in.
Further, the animal bifidobacteria described in step (1) is animal bifidobacteria BB-12.
Further, the Leuconostoc mesenteroides described in step (1) is the bright beading of goldbeater's skin of preserving number CGMCC NO.6432
Bacterium (Leuconostoc mesenteroides) BD01710.
Further, in step (1), the inoculum concentration of the animal bifidobacteria is 106-107CFU/mL, the goldbeater's skin
The inoculum concentration of leukonid BD01710 is 106-107CFU/mL。
Further, in step (1), the ratio between inoculum concentration of the animal bifidobacteria and Leuconostoc mesenteroides is 1:
10-10:1。
Further, in step (2), the mode of the cooling is by way of being stirred after ice bath or turns over the side of cylinder plate
Formula.
Further, in step (1), the raw milk is whole-fat milk powder or skimmed milk powder, the sugarcane of 0-8% by 5-15%
Sugar and complement to 100% water, by dissolving, mix, homogeneous, sterilization, be made after cooling；The percentage is quality percentage
Than.
Further, in step (1), the raw milk is after former milk is standardized, to add 3-8% sucrose and 0-1.5ppm
Manganese gluconate, by dissolving, mix, homogeneous, sterilization, is made after cooling；The percentage is mass percent.
Present invention also offers the acidified milk rich in animal bifidobacteria that a kind of preparation method described above is prepared into.
The positive effect of the present invention is：
1st, the acidified milk provided by the invention rich in animal bifidobacteria, the source for the fermentation medium that preparation process uses
Extensively, of low cost, natural safety；It is found that Leuconostoc mesenteroides BD01710 to animal bifidobacteria by co-culture experiments
Growth-promoting effect, and on this basis by be prepared for the present invention the acidified milk rich in animal bifidobacteria, fermenting
The binary fermentation system based on Leuconostoc mesenteroides and animal bifidobacteria is constructed in product, which can be real in newborn medium
The growth of existing animal bifidobacteria and curdled milk, fermented, animal bifidobacteria viable count is up to 5 × 108More than CFU/mL.
2nd, the acidified milk rich in animal bifidobacteria for preparing of the present invention is full of nutrition, in good taste, preparation method is simply easy
OK, competent animals Bifidobacterium content in acidified milk is high, can effectively play adjust intestinal flora balance and whole after consumption
The prebiotic functions such as intestines effect, have great market application potential and economic value.
3rd, growth of the animal bifidobacteria in numerous food medias is realized；Using animal bifidobacteria as the prebiotic of representative
Bacterium is providing new Technology Ways as the scale application in the food media of representative using breast, in following probiotics fermention breast
Exploitation and probiotics preparation field will have significant application value.
Biomaterial preservation information：
Leuconostoc mesenteroides (Leuconostoc mesenteroides) BD01710 bacterial strains provided by the invention, in
It is deposited within 08 13rd, 2012 China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation
Location：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode：100101.The deposit number of the bacterial strain is：CGMCC No.6432.
Brief description of the drawings
Fig. 1 be the present invention added at the same time in breast animal bifidobacteria and BD01710 and the growth for not adding BD01710 and
Curdled milk comparison diagram.
Embodiment
The present invention is described in further detail by the following examples.There is no specified otherwise such as, it is of the present invention
Various raw materials are commercially available.The step of not illustrating, is this area routine preparation means.
A kind of preparation method of the acidified milk rich in animal bifidobacteria, its preparation method comprise the following steps：
(1) animal bifidobacteria (Bifidobacterium animalis) and the bright string of goldbeater's skin are inoculated with into raw milk respectively
Pearl bacterium (Leuconostoc mesenteroides) BD01710, is uniformly mixed, at 30-37 DEG C of temperature, standing for fermentation
It is 70-95 ° of T to titratable acidity, obtains acidified milk；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 16-
22 DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred in the environment that temperature is 2-10 DEG C and is refrigerated
After-ripening 12-24h, you can the acidified milk of animal bifidobacteria must be rich in.
Preferably, animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) BB-12.Animal
Bifidobacterium breast bifid subspecies Chr.Hansen BB-12 clinical research bifidobacterium strains the most sufficient in the world, it
It is described in more than 300 kinds of scientific publications, wherein more than 130 kinds of publication is related to Human Clinical Study, it is uniquely to obtain full base
Because of the bifidobacterium strain of sequence.The health-care effect of Chr.Hansen BB-12 is in gastrointestinal health and many of immune function
It is verified in clinical research.
The Leuconostoc mesenteroides that Leuconostoc mesenteroides described in step (1) is preserving number CGMCC NO.6432
(Leuconostoc mesenteroides BD01710 is Shanghai Bright Dairy ＆ Food Co., Ltd. to (Leuconostoc mesenteroides) BD01710
Patented strain, its source can be found in notification number be CN 103013891B Chinese patent), hereinafter referred to as BD01710.
Animal bifidobacteria and Leuconostoc mesenteroides BD01710 in preparing raw material, can use the concentration of freeze-drying
Bacterium powder, does not influence protection scope of the present invention.
The present invention promotes to make using growth of the Leuconostoc mesenteroides (Leuconostoc mesenteroides) to probiotics
With growth of the probiotics in food media is realized, growth and curdled milk in especially newborn medium, pass through Leuconostoc mesenteroides
The probiotics fermention product of based on probiotics fermention, high viable count is made in the growth promoting function of BD01710.Used goldbeater's skin
Leukonid BD01710 belongs to leuconostoc mesenteroides subsp mesenteroides (the Leuconostoc mesenteroides of generally recognized as safe
subsp.mesenteroides)。
Leuconostoc mesenteroides BD01710 belongs to by the Ministry of Public Health of China (Ministry of Public Health announce 2012 No. 8) and U.S.'s food
Drug Administration is classified as one of safety edible strain (FDA, 1989).The Leuconostoc mesenteroides BD01710 added can
To produce the dietary fibers such as glucan and levulan (ZL 201310443498.4), these polysaccharide in itself can be as health
Dietary supplements, while bring advantage in extra texture for above-mentioned fermented product.
Further, in step (1), the inoculum concentration of the animal bifidobacteria is 106-107CFU/mL, the goldbeater's skin
The inoculum concentration of leukonid BD01710 is 106-107CFU/mL；Or the animal bifidobacteria and Leuconostoc mesenteroides connect
The ratio between kind amount is 1:10-10:1.
Further, in step (1), the raw milk be this area conventional selection, by 5-10% whole-fat milk powder or
Skimmed milk powder, 0-5% sucrose and complement to 100% water, by dissolving, mix, homogeneous, sterilization, be made after cooling, preferably
Whole-fat milk powder or skimmed milk powder, the 5% sucrose and water for complementing to 100% that ground is 10%, by mixed raw material preheating liter
Temperature, carries out homogeneous under 60-70 DEG C, 18-22MPa, and then at 90-95 DEG C, insulation sterilization 5-10mi n, are subsequently cooled to
40-45 DEG C, raw milk is made；The percentage is mass percent.
Or the raw milk is after former milk is standardized, and adds 3-8% sucrose and 0-1.5ppm manganese gluconates, process are molten
It is made after solution, mixing, homogeneous, sterilization, cooling, preferably 5% sucrose and 1.0ppm manganese gluconates, raw material is uniformly mixed
Pre-heating temperature elevation afterwards, carries out homogeneous under the conditions of 60-70 DEG C, 18-22MPa, then at 90-95 DEG C, insulation sterilization 5-10min,
It is subsequently cooled to 35-42 DEG C, it is stand-by；The percentage is mass percent.Raw milk is suitable for acidified milk system using other
The dairy sources of product.
Present invention also offers the acidified milk rich in animal bifidobacteria that a kind of preparation method described above is prepared into.
Embodiment 1
Raw material prepares：Raw milk, by 10% whole-fat milk powder or skimmed milk powder, 5% sucrose and complements to 100%
Water, by fully dissolving, be uniformly mixed, homogeneous (60 DEG C, 22MPa), 95 DEG C sterilization 5mi n, be cooled to 40 DEG C it is stand-by.
(1) animal bifidobacteria BB-12 (10 is inoculated with into raw milk respectively7) and Leuconostoc mesenteroides CFU/mL
BD01710(106CFU/mL), it is uniformly mixed, at 30 DEG C of temperature, it is 70 ° of T to be left to ferment to titratable acidity, must be fermented
Breast；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 16
DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred in the environment that temperature is 2 DEG C and carries out refrigeration after-ripening
12h, you can the acidified milk of animal bifidobacteria must be rich in.
Above-mentioned acidified milk sample is sampled, is counted after gradient dilution with colony counting method.Obtain result such as
Table 1, the content of active plant lactobacillus is higher than 5 × 10 in the acidified milk8CFU/mL。
Table 1：Viable count in pH value and acidified milk rich in animal bifidobacteria acidified milk
Embodiment 2
Raw material prepares：Raw milk, by 10% whole-fat milk powder or skimmed milk powder, 5% sucrose and complements to 100%
Water, by fully dissolving, be uniformly mixed, homogeneous (70 DEG C, 22MPa), 95 DEG C sterilization 10min, be cooled to 45 DEG C it is stand-by.
(1) animal bifidobacteria (10 is inoculated with into raw milk respectively6) and Leuconostoc mesenteroides BD01710 CFU/mL
(107CFU/mL), it is uniformly mixed, at 37 DEG C of temperature, it is 95 ° of T to be left to ferment to titratable acidity, obtains acidified milk；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 22
DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred to after being refrigerated in the environment that temperature is 10 DEG C
Ripe 24h, you can the acidified milk of animal bifidobacteria must be rich in.
Above-mentioned acidified milk sample is sampled, is counted after gradient dilution with colony counting method.Obtain result such as
Table 2, it is seen that the content of competent animals Bifidobacterium is higher than 5 × 10 in the acidified milk8CFU/mL。
Table 2：Viable count in pH value and acidified milk rich in animal bifidobacteria acidified milk
Embodiment 3
Raw material prepares：Raw milk, by 10% whole-fat milk powder or skimmed milk powder, 5% sucrose and complements to 100%
Water, by fully dissolving, be uniformly mixed, homogeneous (70 DEG C, 22MPa), 95 DEG C sterilization 10min, be cooled to 45 DEG C it is stand-by.
(1) animal bifidobacteria (10 is inoculated with into raw milk respectively7CFU/mL) and Leuconostoc mesenteroides BD01710 (3 ×
106CFU/mL), it is uniformly mixed, at 37 DEG C of temperature, it is 95 ° of T to be left to ferment to titratable acidity, obtains acidified milk；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 22
DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred to after being refrigerated in the environment that temperature is 10 DEG C
Ripe 24h, you can the acidified milk of animal bifidobacteria must be rich in.
Above-mentioned acidified milk sample is sampled, is counted after gradient dilution with colony counting method.Obtain result such as
Table 3, it is seen that the content of competent animals Bifidobacterium is higher than 5 × 10 in the acidified milk8CFU/mL。
Table 3：Viable count in pH value and acidified milk rich in animal bifidobacteria acidified milk
Embodiment 4
Raw material prepares：Raw milk is after former milk is standardized, 5% sucrose and 1.0ppm manganese gluconates to be added, by raw material
Pre-heating temperature elevation after mixing, carries out homogeneous under the conditions of 60 DEG C, 18MPa, then at 90 DEG C, insulation sterilization 10min, then
It is cooled to 42 DEG C.
(1) animal bifidobacteria BB-12 (10 is inoculated with into raw milk respectively7) and Leuconostoc mesenteroides CFU/mL
BD01710(106CFU/mL), it is uniformly mixed, at 30 DEG C of temperature, it is 70 ° of T to be left to ferment to titratable acidity, must be fermented
Breast；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 16
DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred in the environment that temperature is 2-10 DEG C and is refrigerated
After-ripening 12h, you can the acidified milk of animal bifidobacteria must be rich in.
Above-mentioned acidified milk sample is sampled, is counted after gradient dilution with colony counting method.Obtain result such as
Table 4, it is seen that the content of competent animals Bifidobacterium is higher than 10 in the acidified milk9CFU/mL。
Table 4：Viable count in pH value and acidified milk rich in animal bifidobacteria acidified milk
Embodiment 5
Raw material prepares：Raw milk is after former milk is standardized, 5% sucrose and 1.0ppm manganese gluconates to be added, by raw material
Pre-heating temperature elevation after mixing, carries out homogeneous under the conditions of 60 DEG C, 18MPa, then at 90 DEG C, insulation sterilization 10min, then
It is cooled to 35 DEG C.
(1) animal bifidobacteria (10 is inoculated with into raw milk respectively6) and Leuconostoc mesenteroides BD01710 CFU/mL
(107CFU/mL), it is uniformly mixed, at 37 DEG C of temperature, it is 95 ° of T to be left to ferment to titratable acidity, obtains acidified milk；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 22
DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred to after being refrigerated in the environment that temperature is 10 DEG C
Ripe 24h, you can the acidified milk of animal bifidobacteria must be rich in.
Above-mentioned acidified milk sample is sampled, is counted after gradient dilution with colony counting method.Obtain result such as
Table 5, it is seen that the content of competent animals Bifidobacterium is higher than 10 in the acidified milk9CFU/mL。
Table 5：Viable count in pH value and acidified milk rich in animal bifidobacteria acidified milk
Embodiment 6
Raw material prepares：Raw milk is after former milk is standardized, 5% sucrose and 1.0ppm manganese gluconates to be added, by raw material
Pre-heating temperature elevation after mixing, carries out homogeneous under the conditions of 60 DEG C, 18MPa, then at 90 DEG C, insulation sterilization 10min, then
It is cooled to 40 DEG C.
(1) animal bifidobacteria (10 is inoculated with into raw milk respectively7CFU/mL) and Leuconostoc mesenteroides BD01710 (3 ×
106CFU/mL), it is uniformly mixed, at 37 DEG C of temperature, it is 95 ° of T to be left to ferment to titratable acidity, obtains acidified milk；
(2) acidified milk made from step (1) by way of being stirred after ice bath or is turned over the mode of cylinder plate and is cooled to 22
DEG C, obtain cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred to after being refrigerated in the environment that temperature is 10 DEG C
Ripe 24h, you can the acidified milk of animal bifidobacteria must be rich in.
Above-mentioned acidified milk sample is sampled, is counted after gradient dilution with colony counting method.Obtain result such as
Table 6, it is seen that the content of competent animals Bifidobacterium is higher than 10 in the acidified milk9CFU/mL。
Table 6：Viable count in pH value and acidified milk rich in animal bifidobacteria acidified milk
As shown in Figure 1, for embodiment 1, while it is vaccinated with animal bifidobacteria BB-12 and Leuconostoc mesenteroides
The acidified milk of BD01710 and the acidified milk for not being inoculated with Leuconostoc mesenteroides BD01710, animal bifid in the acidified milk of embodiment 1
The strain amount of bacillus significantly improves, and curdled milk positive effect.Animal bifidobacteria BB-12 can be realized in the newborn medium of the present invention
Growth and curdled milk, through 24 it is small when ferment, the number of active bifid bacteria is up to 5 × 108CFU/mL。
It is to be understood that after the above of the present invention has been read, those skilled in the art can be the present invention various
Change or modification, such equivalent forms are equally rained the scope of the appended claims of the present application.
Claims (9)
1. a kind of preparation method of the acidified milk rich in animal bifidobacteria, it is characterised in that its preparation method includes following step
Suddenly：
(1) animal bifidobacteria (Bifidobacterium animalis) and Leuconostoc mesenteroides are inoculated with into raw milk respectively
(Leuconostoc mesenteroides) BD01710, is uniformly mixed, at 30-37 DEG C of temperature, standing for fermentation to drop
It is 70-95 ° of T to determine acidity, obtains acidified milk；
(2) acidified milk made from step (1) is cooled to 16-22 DEG C, obtains cooling and fermentation breast；
(3) it is cooling and fermentation breast is filling into packing container, then it is transferred in the environment that temperature is 2-10 DEG C and carries out refrigeration after-ripening
12-24h, you can the acidified milk of animal bifidobacteria must be rich in.
2. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (1)
Described in animal bifidobacteria be animal bifidobacteria BB-12.
3. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (1)
Described in Leuconostoc mesenteroides be preserving number CGMCC NO.6432 Leuconostoc mesenteroides (Leuconostoc
mesenteroides)BD01710。
4. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (1)
In, the inoculum concentration of the animal bifidobacteria is 106-107CFU/mL, the inoculum concentration of the Leuconostoc mesenteroides BD01710 are
106-107CFU/mL。
5. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (1)
In, the ratio between inoculum concentration of the animal bifidobacteria and Leuconostoc mesenteroides is 1:10-10:1.
6. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (2)
In, the mode of the cooling is by way of being stirred after ice bath or turns over the mode of cylinder plate.
7. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (1)
In, the raw milk is by the whole-fat milk powder or skimmed milk powder of 5-10%, the sucrose of 0-5% and the water for complementing to 100%, is passed through
It is made after dissolving, mixing, homogeneous, sterilization, cooling；The percentage is mass percent.
8. the preparation method of the acidified milk rich in animal bifidobacteria as claimed in claim 1, it is characterised in that step (1)
In, the raw milk is after former milk is standardized, 3-8% sucrose and 0-1.5ppm manganese gluconates to be added, by dissolving, mixing
It is made after conjunction, homogeneous, sterilization, cooling；The percentage is mass percent.
A kind of 9. acidified milk rich in animal bifidobacteria being prepared into such as claim 1-8 any one of them preparation methods.
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| CN112219899A (en) * | 2020-10-16 | 2021-01-15 | 光明乳业股份有限公司 | Bifidobacterium animalis fermented milk and preparation method thereof |
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