CN107941797A - A kind of visual colorimetric determination sensor for detecting mercury ion - Google Patents
A kind of visual colorimetric determination sensor for detecting mercury ion Download PDFInfo
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- CN107941797A CN107941797A CN201711311307.3A CN201711311307A CN107941797A CN 107941797 A CN107941797 A CN 107941797A CN 201711311307 A CN201711311307 A CN 201711311307A CN 107941797 A CN107941797 A CN 107941797A
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- dna
- concentration
- exonuclease iii
- colorimetric determination
- mercury ion
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The present invention provides a kind of visual colorimetric determination sensor for detecting mercury ion, and H DNA of the design with special hairpin structure, in the presence of mercury ion, the 3 ' ends and 5 ' ends of H DNA pass through T Hg2+T complementary structures match, and make the stem part complete complementary of H DNA, trigger exonuclease III to shear it, and DNA of the generation rich in guanine is single-stranded, discharges Hg2+Again and H DNA reactions trigger endonuclease reaction, so circulation, H DNA is constantly sheared, generate more and more single stranded DNAs rich in G, in the presence of hemin, form DNA catalyzing enzymes, be catalyzed 3,3', 5,5' tetramethyl benzidines and H2O2Reaction, generates blue product, realizes Visual retrieval mercury ion.The method of the present invention is easy to operate, cost is low, and the time is short, mercury ion content that can rapidly in Visual retrieval environmental water sample, is expected to the detection applied to environmental water sample mercury.
Description
Technical field
Recycled the present invention relates to one kind based on exonuclease III auxiliary marks and amplify letter jointly with DNA catalyzing enzymes
Number visual colorimetric determination sensor detection mercury ion method, belong to analytical chemistry field.
Background technology
The distribution of mercury and mercury compound is very extensive, is a poisonous huge sum of money maximum to the mankind and the living environment harm of mankind institute
One of belong to.It is applied to due to industrial using the toxicity of mercury in the industrial products such as preservative, pesticide, antiseptic so that
People contact mercury and the probability of mercury compound greatly increases.Mercury ion can be also caused the health of people even in concentration is very low
Grave danger.With the raising of people's living standard and health perception, more and more people begin to focus on mercury to health
Harm.Some researches show that the heavy metal element that mercury is possible to the motion function that can cause people and memory capability is deteriorated, sucked
The mercury of amount is possible to that the renal system of people, nervous system, immune system, reproductive system etc. can be caused impaired；In addition, mercury and mercuration
Compound can enter human body by number of ways such as such as empty gas and water, foods, and mercury has bioconcentration, into human body
Mercury also is difficult to discharge, therefore harm to the human body is very big.
With the fast development of Modern Testing, more and more mercury ion detecting methods come out, common at present
Mercury ion analysis test method be mainly instrumental method, such as inductively coupled plasma atomic emission spectrometry（ICP-
AES）, inductively coupled plasma mass spectrometry, atomic absorption spectrography (AAS)（AAS）, cold steam atomic fluorescence spectrometry (CV-AFS)
Deng.These traditional detection methods are since complicated, sample detection environmental requirement is high, expensive equipment and can not detect on the spot etc. former
Cause so that their application is restricted.With the fast development of Modern Testing, more and more new method quilts
It is applied in the analysis detection of mercury, such as is had been widely used for the methods of electrochemical method, fluorescent method and colorimetric detection method
The detection of mercury ion.Colorimetric method is received significant attention due to the features such as its result is obvious, simple, quick and high sensitivity.
The content of the invention
It is an object of the invention to provide a kind of visual colorimetric determination sensor for detecting mercury ion, this colorimetric sensor detection
Method greatly improves detection using the strategy of the circulation of exonuclease III auxiliary marks and the common amplified signal of DNA catalyzing enzymes
Sensitivity.A kind of H-DNA of hairpin structure is designed, in the presence of mercury ion, its stem part meeting complementary pairing, triggers nucleic acid
ExonucleaseⅢ shears it, and the single stranded DNA rich in guanine of generation, can form DNA in the presence of hemin
Catalyzing enzyme, is catalyzed 3,3', 5,5'- tetramethyl benzidines（TMB）With H2O2Reaction, generate blue material, by observing solution face
The significant change of color judges that target analytes mercury ion whether there is, and content number, traditional analysis inspection can be solved
The problems such as survey method detects the complicated of mercury ion, requirement is high, testing cost is high and background interference is big.The detection side of the present invention
Method is easy to operate, cost is low, and the time is short, mercury ion content that can rapidly in Visual retrieval environmental water sample.
To achieve these goals, the present invention adopts the following technical scheme that：
A kind of visual colorimetric determination sensor based on exonuclease III and DNA catalyzing enzyme amplified signal, by the H- of hairpin structure
DNA, hemin, exonuclease III, 3,3', 5,5'- tetramethyl benzidines and H2O2Composition.
The sequence of the H-DNA is：
5’-CTTTAGGGTGGGGAGGGTGGGGCCCCACCCTTTTG -3’。
The preparation method of the hairpin structure H-DNA is：H-DNA is dissolved in containing 100 mM NaCl and 5 mM MgCl2
20 mM pH, 7.4 Tris-HCl buffer solutions 1 in, heated 10 minutes under the conditions of 90 DEG C, naturally cool to room temperature.The hair
The concentration of clamping structure H-DNA is 2.5 μM.
The concentration of the hemin is 7.75 μM, and preparation method is：Hemin sample is dissolved in diformazan Asia
Sulfone, is then diluted to required concentration with Tris-HCl buffer solutions 2；Tris-HCl buffer solutions 2 contain 300 mM Tris-HCl, 10mM
TCEP, 1 M NaCl, 1mM EDTA and 1 mM MgCl2, pH 7.4.
The concentration of the exonuclease III is 2.5 U/ μ L.
Described 3,3', the concentration of 5,5'- tetramethyl benzidines is 5.0 mM, H2O2Concentration be 10.0 mM；The 3,3',
The preparation method of 5,5'- tetramethyl benzidines is：First 3,3', 5,5'- tetramethyl benzidine samples are dissolved in straight alcohol, then
Add ultra-pure water constant volume, with by 10 mM NaH2PO4With 10 mM Na2HPO4Needed for 5.5 PBS buffer of pH of composition is diluted to
Concentration.The H2O2Preparation method be to dilute 30% hydrogen peroxide to required concentration with ultra-pure water.
A kind of visual ratio recycled based on exonuclease III auxiliary marks with the common amplified signal of DNA catalyzing enzymes
The method that colour sensor detects mercury ion, follows the steps below：
（1）45 μ L concentration are taken respectively as 2.5 μM of H-DNA and the Hg of 45 μ L various concentrations2+Titer, is uniformly mixed, 2 min
Add the exonuclease III (Exo-III) that 10 μ L concentration are 2.5 U/ μ L thereto afterwards, it is economical to be put into K30 after mixing
In dry-type thermostat, 40 min are reacted under the conditions of 35 DEG C;
（2）After treating above-mentioned reaction solution cooling, the hemin that 100 μ L concentration are 7.75 μM is added thereto
（hemin）, refrigerator is put into after mixing（4℃）React 30 min;
（3）The 3,3' that 100 μ L concentration are 5.0mM, 5,5'- tetramethyl benzidines are separately added into above-mentioned reaction system（TMB）
With the H that 100 μ L concentration are 10.0 mM2O2, solution colour change is observed after mixing 1 min of mixing.
Step（1）In the buffer solution of H-DNA solution be Tris-HCl buffer solutions 1 (TB1), by 20 mM Tris-HCl,
100 mM NaCl and 5 mM MgCl2（pH 7.4）Composition, H-DNA is dissolved in TB1, is heated 10 minutes under the conditions of 90 DEG C,
Then room temperature is naturally cooled to.
Step（2）In hemin（hemin）Dilution buffer be by 300 mM Tris-HCl, 10mM
TCEP, 1 M NaCl, 1mM EDTA and 1 mM MgCl2The Tris-HCl buffer solutions 2 of composition（pH 7.4）（TB2）, first by chlorine
Change ferroheme and be dissolved in dimethyl sulfoxide（DMSO）In, then required concentration is diluted to TB2.
Step（3）In 3,3', 5,5'- tetramethyl benzidines（TMB）Dilution buffer be by 10 mM NaH2PO4With
10 mM Na2HPO4The PBS buffer of composition（pH 5.5）, first TMB samples are dissolved in straight alcohol, then plus ultra-pure water is determined
Hold, use PBS buffer（pH 5.5）It is diluted to required concentration.
The Hg2+The preparation method of titer is by Hg2+Titer storing solution mass fraction is 0.5% HNO3Dilution
To required concentration.
Actual sample is handled：After ambient water sample is stood a period of time, with 0.22 μm of filtering with microporous membrane,
Add dust technology into filtered fluid, make HNO in solution3Ultimate density be 0.5%, be uniformly mixed.
The remarkable advantage of the present invention：
（1）Construct a kind of nothing recycled based on exonuclease III auxiliary marks with the common amplified signal of DNA catalyzing enzymes
Mark, overdelicate mercury ion visual colorimetric determination sensor.In the presence of mercury ion, the stem part complete complementary of H-DNA, triggers
Exonuclease III shears it, and generation is rich in guanine（G）DNA it is single-stranded, in hemin（hemin）In the presence of, meeting
DNA catalyzing enzymes are formed, 3,3', 5,5'- tetramethyl benzidines can be catalyzed（TMB）With H2O2Reaction, generate blue product.Mercury
Ion concentration is different, and the weight of solution colour is different, by naked eyes can half-quantitative detection mercury ion without by
Instrument detect, therefore it is easy to operate, cost is low；
（2）H-DNA used in the present invention, which need not carry out modification and signal mark, can detect mercury ion；
（3）3,3' used in the present invention, 5,5'- tetramethyl benzidine（TMB）It is the thing that a kind of property is stable, nontoxic
Matter, relatively safety, therefore the visual colorimetric determination sensor of the present invention is environmentally friendly；
（4）The present invention visual colorimetric determination sensor detection mercury ion concentration limit as low as 2.0 fM, detection range be
2.0 fM-50 nM；
(5) mercury ion visual colorimetric determination sensor specificity of the invention is good, and reaction is gentle, and detection speed is fast, favorable reproducibility, behaviour
Make simplicity, mercury ion can be detected with quick visualization.
Brief description of the drawings
Fig. 1 visual colorimetric determinations sensor detects the schematic diagram of mercury ion, under the conditions of existing for mercury ion, the 3 ' ends of H-DNA
With 5 ' end pass through T Hg2+T complementary structures match so that the stem part complete complementary of H-DNA, triggers exonuclease
III shears it, and DNA of the generation rich in guanine is single-stranded, discharges Hg2+It can be reacted again with H-DNA, trigger digestion anti-
Should, so circulation so that H-DNA is constantly sheared, and generation is more and more rich in the single stranded DNA of G, in hemin
（hemin）In the presence of, DNA catalyzing enzymes can be formed, are catalyzed 3,3', 5,5'- tetramethyl benzidines（TMB）With H2O2Reaction, it is raw
Au bleu product.
The visual colorimetric determination sensor of Fig. 2 present invention is to the colour developing figure of the mercury ion standard solution of various concentrations, mercury ion mark
The concentration of quasi- solution is followed successively by 0.0 fM, 2.0 fM, 20 fM, 0.2 pM, 2.0 pM, 20 pM, 0.2 nM, 2.0 nM, 20
NM, 50 nM, figure it is seen that with the increase of mercury ion concentration of standard solution, solution blueness is more and more deeper, therefore can be with
The depth for observing by the naked eye solution colour judges the concentration ranges scope of mercury ion.
The colorimetric sensor of Fig. 3 present invention is followed successively by 0.0 to the colour developing figure of the mercury ion standard solution of low concentration, concentration
FM, 2.0 fM, 20 fM, 2.0 pM, 2.0 nM, by Fig. 3 this it appears that in mercury ion low concentration, the blueness change of solution
Change clearly.
Fig. 4 is that the selectivity of mercury ion detecting is tested for the colorimetric sensor of the present invention.Colorimetric sensor is to Hg2+（20
nM）With 10 kinds of disturbance ion (Ca2+, Mn2+, Mg2+, Zn2+, Cu2+, Co2+, Bi3+, Pb2+, Ba2+, Ni2+)
(2.0 μM) have differential responses, and only mercury ion can just make solution become au bleu, illustrate the visual colorimetric determination sensor to mercury ion
With good selectivity.
Fig. 5 is tried for the detection of colorimetric sensor of the invention to the actual sample of actual sample and different spiked levels
Test.Be successively from left to right sensor to blank sample, originally water sample, originally the fM of water sample+1.0, originally water sample+
1.0 pM, the originally nM of water sample+1.0, lake water sample ,+1.0 fM of lake water sample ,+1.0 pM of lake water sample, lake water sample+
The colour developing figure of 1.0 nM, as shown in Figure 5 with the increase of actual sample spiked levels, solution blueness is more and more deeper, illustrates this hair
Bright visual colorimetric determination sensor can detect the mercury ion in environmental water sample.
Embodiment
Embodiment 1
1st, the preparation of hairpin structure H-DNA：
（1）Tris-HCl buffer solutions 1 (TB1, pH 7.4) are configured, TB1 is by 20 mM Tris-HCl, 100 mM NaCl and 5
mM MgCl2Composition；
（2）H-DNA is dissolved in TB1, is heated 10 minutes under the conditions of 90 DEG C, then naturally cools to room temperature.
2nd, hemin（hemin）The preparation of solution：
（1）Configure Tris-HCl dilution buffers 2 (TB2, pH 7.4), TB2 by 300 mM Tris-HCl, 10mM TCEP,
1 M NaCl, 1mM EDTA and 1 mM MgCl2Composition；
（2）Hemin is first dissolved in dimethyl sulfoxide（DMSO）In, then it is diluted to Tris-HCl buffer solutions 2 required
Concentration.
3rd, 3,3', 5,5'- tetramethyl benzidine（TMB）The preparation of solution：
（1）Configure PBS buffer（pH 5.5）, PBS buffer is by 10 mM NaH2PO4, 10 mM Na2HPO4Composition；
（2）First TMB samples are dissolved in straight alcohol, then adds ultra-pure water constant volume, uses PBS buffer（pH 5.5）Needed for being diluted to
Concentration.
Embodiment 2
The pre-treatment of actual sample：
（1）After ambient water sample is stood a period of time, with 0.22 μm of filtering with microporous membrane, dilute nitre is added into filtered fluid
Acid, makes HNO in sample solution3Ultimate density be 0.5%, be uniformly mixed.
（2）It originally water sample and lake water sample will respectively be divided into 3 parts, be numbered to them：Tap water 1, tap water 2, from
Water 3 and lake water 1, lake water 2, lake water 3, while add standard Hg to tap water 1 and lake water 12+Solution is made into
Spiked levels are 1.0 fM, and standard Hg is added to tap water 2 and lake water 22+Solution, it is 1.0 pM to be made into spiked levels；Give
Tap water 3 and lake water 3 add standard Hg2+Solution, it is 1.0 nM to be made into spiked levels.
Embodiment 3
The detection of actual sample：
（1）The H-DNA and 45 μ L water samples that 45 μ L concentration are 2.5 μM are taken respectively（Or the water sample mark-on solution of various concentrations）, mix
The exonuclease III (Exo-III) for uniformly, adding that 10 μ L concentration are 2.5 U/ μ L after 2 min thereto is closed, is put after mixing
Enter the economical dry-type thermostats of K30,40 min are reacted under the conditions of 35 DEG C;
（2）After treating above-mentioned reaction solution cooling, the hemin that 100 μ L concentration are 7.75 μM is added thereto
（hemin）, refrigerator is being put into after mixing（4℃）React 30 min;
（3）The 3,3' that 100 μ L concentration are 5.0mM, 5,5'- tetramethyl benzidines are separately added into above-mentioned reaction system（TMB）
With the H that 100 μ L concentration are 10.0 mM2O2, solution colour change is observed after mixing 1 min of mixing.
Embodiment 4
Actual sample testing result as shown in figure 5, the visual colorimetric determination sensor of the present invention to the sample solutions of different spiked levels
（It is left → right：0.0th, originally water sample, the originally fM of water sample+1.0, the originally pM of water sample+1.0, originally water sample+1.0
NM, lake water sample ,+1.0 fM of lake water sample ,+1.0 pM of lake water sample ,+1.0 nM of lake water sample）Colour developing figure, can by Fig. 5
Know, visual colorimetric determination sensor of the invention can detect the mercury ion in environmental water sample.
SEQUENCE LISTING
<110>University of Fuzhou
<120>A kind of visual colorimetric determination sensor for detecting mercury ion
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 35
<212> DNA
<213> H-DNA
<400> 1
ctttagggtg gggagggtgg ggccccaccc ttttg 35
Claims (10)
- A kind of 1. visual colorimetric determination sensor based on exonuclease III and DNA catalyzing enzyme amplified signal, it is characterised in that：Institute Sensor is stated by the H-DNA of hairpin structure, hemin, exonuclease III, 3,3', 5,5'- tetramethyl benzidines with H2O2Composition.
- 2. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 1 passes Sensor, it is characterised in that：The sequence of the H-DNA is：5’-CTTTAGGGTGGGGA GGGTGG GGCCCCACCCTTTTG - 3’。
- 3. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 1 passes Sensor, it is characterised in that：The preparation method of the hairpin structure H-DNA is：H-DNA is dissolved in containing 100 mM NaCl and 5 mM MgCl220 mM pH, 7.4 Tris-HCl buffer solutions 1 in, heated 10 minutes under the conditions of 90 DEG C, naturally cool to room Temperature.
- 4. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 3 passes Sensor, it is characterised in that：The concentration of the hairpin structure H-DNA is 2.5 μM.
- 5. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 1 passes Sensor, it is characterised in that：The concentration of the hemin is 7.75 μM, and preparation method is：Hemin sample is molten In dimethyl sulfoxide, then required concentration is diluted to Tris-HCl buffer solutions 2；Tris-HCl buffer solutions 2 contain 300 mM Tris- HCl, 10mM TCEP, 1 M NaCl, 1mM EDTA and 1 mM MgCl2, pH 7.4.
- 6. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 1 passes Sensor, it is characterised in that：The concentration of the exonuclease III is 2.5 U/ μ L.
- 7. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 1 passes Sensor, it is characterised in that：Described 3,3', the concentration of 5,5'- tetramethyl benzidines is 5.0 mM, H2O2Concentration be 10.0 mM； The preparation method of the 3,3', 5,5'- tetramethyl benzidine is：3,3', 5,5'- tetramethyl benzidines are first dissolved in straight alcohol In, then add ultra-pure water constant volume, with by 10 mM NaH2PO4With 10 mM Na2HPO45.5 PBS buffer of pH of composition is dilute Release required concentration；The H2O2Preparation method be to dilute 30% hydrogen peroxide to required concentration with ultra-pure water.
- A kind of 8. visual colorimetric determination sensing based on exonuclease III and DNA catalyzing enzyme amplified signal as claimed in claim 1 The method that device detects mercury ion, it is characterised in that：Comprise the following steps：（1）45 μ L concentration are taken respectively as 2.5 μM of H-DNA and the Hg of 45 μ L various concentrations2+Titer, is uniformly mixed, 2 The exonuclease III that 10 μ L concentration are 2.5 U/ μ L is added after min thereto, is put into after mixing in thermostat, at 35 DEG C Under the conditions of react 40 min；（2）After treating above-mentioned reaction solution cooling, the hemin that 100 μ L concentration are 7.75 μM is added thereto, is mixed 4 DEG C of refrigerators are put into after even and react 30 min；（3）To above-mentioned reaction system be separately added into 100 μ L concentration be 5.0 mM 3,3', 5,5'- tetramethyl benzidines and 100 μ L concentration are the H of 10.0 mM2O2, solution colour change is observed after mixing 1 min of mixing.
- 9. a kind of visual colorimetric determination based on exonuclease III and DNA catalyzing enzyme amplified signal according to claim 8 passes The method that sensor detects mercury ion, it is characterised in that：The Hg2+The preparation method of titer is：It is dense needed for dust technology is prepared The mercury titer of degree so that the HNO in mercury ion standard solution3Concentration is 0.5wt%.
- A kind of 10. application of the visual colorimetric determination sensor in environment water sample analysis as claimed in claim 1.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676844A (en) * | 2018-05-24 | 2018-10-19 | 中国农业大学 | A kind of structure of double enzyme amplification mercury ion biosensors |
| CN109444397A (en) * | 2018-10-31 | 2019-03-08 | 重庆工商大学 | A kind of detection method of mercury ion |
| CN111487242A (en) * | 2020-04-27 | 2020-08-04 | 天津工业大学 | Hydrogen peroxide detection method based on iron porphyrin two-dimensional MOFs enzyme catalysis |
| CN111693518A (en) * | 2019-03-14 | 2020-09-22 | 重庆工商大学 | Mercury ion detection method |
| CN112924406A (en) * | 2021-02-02 | 2021-06-08 | 湘潭大学 | Mimic enzyme-assisted mercury ion detection method and kit |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120040867A1 (en) * | 2009-01-28 | 2012-02-16 | The Trustees Of Columbia University In The City Of New York | Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes |
| CN104818324A (en) * | 2015-04-14 | 2015-08-05 | 南昌大学 | DNA colorimetric logic gate construction method based on metal ion regulation and control of exonuclease III shearing action |
| CN104946770A (en) * | 2015-07-02 | 2015-09-30 | 常熟理工学院 | Novel exonuclease-III-based mercury ion detection method |
| CN105132524A (en) * | 2015-09-25 | 2015-12-09 | 山东大学 | Application of dual application reaction of Exo (exonuclease) III-assisted cycle and DNAzyme cycle to Hg<2+> detection |
| CN106191042A (en) * | 2016-07-16 | 2016-12-07 | 湖南工程学院 | Two-way Cycle series signals based on exonuclease III auxiliary amplifies DNA combination probe compositions and preparation method and application |
| CN106872682A (en) * | 2017-02-17 | 2017-06-20 | 济南大学 | A kind of colorimetric bio sensor for detecting mercury ion and preparation method thereof |
-
2017
- 2017-12-11 CN CN201711311307.3A patent/CN107941797B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120040867A1 (en) * | 2009-01-28 | 2012-02-16 | The Trustees Of Columbia University In The City Of New York | Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes |
| CN104818324A (en) * | 2015-04-14 | 2015-08-05 | 南昌大学 | DNA colorimetric logic gate construction method based on metal ion regulation and control of exonuclease III shearing action |
| CN104946770A (en) * | 2015-07-02 | 2015-09-30 | 常熟理工学院 | Novel exonuclease-III-based mercury ion detection method |
| CN105132524A (en) * | 2015-09-25 | 2015-12-09 | 山东大学 | Application of dual application reaction of Exo (exonuclease) III-assisted cycle and DNAzyme cycle to Hg<2+> detection |
| CN106191042A (en) * | 2016-07-16 | 2016-12-07 | 湖南工程学院 | Two-way Cycle series signals based on exonuclease III auxiliary amplifies DNA combination probe compositions and preparation method and application |
| CN106872682A (en) * | 2017-02-17 | 2017-06-20 | 济南大学 | A kind of colorimetric bio sensor for detecting mercury ion and preparation method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676844A (en) * | 2018-05-24 | 2018-10-19 | 中国农业大学 | A kind of structure of double enzyme amplification mercury ion biosensors |
| CN108676844B (en) * | 2018-05-24 | 2021-04-06 | 中国农业大学 | Construction of double-enzyme amplified mercury ion biosensor |
| CN109444397A (en) * | 2018-10-31 | 2019-03-08 | 重庆工商大学 | A kind of detection method of mercury ion |
| CN109444397B (en) * | 2018-10-31 | 2023-04-18 | 重庆工商大学 | Mercury ion detection method |
| CN111693518A (en) * | 2019-03-14 | 2020-09-22 | 重庆工商大学 | Mercury ion detection method |
| CN111693518B (en) * | 2019-03-14 | 2022-08-05 | 重庆工商大学 | Mercury ion detection method |
| CN111487242A (en) * | 2020-04-27 | 2020-08-04 | 天津工业大学 | Hydrogen peroxide detection method based on iron porphyrin two-dimensional MOFs enzyme catalysis |
| CN112924406A (en) * | 2021-02-02 | 2021-06-08 | 湘潭大学 | Mimic enzyme-assisted mercury ion detection method and kit |
| CN112924406B (en) * | 2021-02-02 | 2022-08-12 | 湘潭大学 | Mimic enzyme-assisted mercury ion detection method and kit |
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| CN107941797B (en) | 2019-12-17 |
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