CN107941778A - A kind of sensor and its methods for making and using same for being used to detect body fluid pH - Google Patents
A kind of sensor and its methods for making and using same for being used to detect body fluid pH Download PDFInfo
- Publication number
- CN107941778A CN107941778A CN201710943779.4A CN201710943779A CN107941778A CN 107941778 A CN107941778 A CN 107941778A CN 201710943779 A CN201710943779 A CN 201710943779A CN 107941778 A CN107941778 A CN 107941778A
- Authority
- CN
- China
- Prior art keywords
- body fluid
- sensor
- substrate
- detect body
- metal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Abstract
The invention discloses a kind of sensor and its methods for making and using same for being used to detect body fluid pH.This sensor for being used to detect body fluid pH, including metal Nano structure substrate, metal Nano structure substrate are wrapped with hydrogen ion concentration sensitive material.The preparation method and application process of this sensor for being used to detect body fluid pH are also disclosed that at the same time.The biology sensor based on localised plasmon resonance principle of the invention have the advantages that high throughput, detecting instrument simply, reliable results and cheap, by the way that the material layer of hydrogen ion concentration sensitivity is coated on metal Nano structure, quick detection and monitoring available for human body fluid pH.Sensor of the invention technology of preparing is simple, of low cost and can integrate, suitable for producing in enormous quantities.
Description
Technical field
The present invention relates to a kind of sensor and its methods for making and using same for being used to detect body fluid pH.
Background technology
The acid-base value (pH value) of human body fluid can reflect a variety of Biochemical Informations.For example the pH value of local wound microenvironment can pass through shadow
Ringing many aspects such as tissue oxygen evolution, revascularization, bacterial virulence, enzymatic activity influences wound healing, by detecting and monitoring pH
Value, adjusts the acid-base value of local wound microenvironment, peomotes refractory wounds (wound, burn, pressure sore, diabetes related ulcers)
Reparation.For another example, saliva pH can reflect the pathological and physiological condition in oral cavity, low pH and dentine, the burn into mucosal inflammation of enamel
Correlation, and the stability and resistance to corrosion of oral cavity titanium prosthesis surface film oxide can be reduced, detection oral pH helps
In the early prevention and treatment of mouth disease.In addition, the pH value detection of gastric juice can reflect part gastrointestinal disease state and therapeutic effect, gastric juice
PH value detection and monitoring clinically possess more important clinical meaning.
There are electrochemical method and optical means currently for the detection method of body fluid pH.Electrochemical method result is easily subject to
The interference of solion, and electrode easily pollutes.Compared to electrochemical method, optical means is more suitable for the detection of pH.Detect saliva
The optical means of liquid pH includes fluorescence method and chemoluminescence method., it is necessary to more complicated light path system in fluorescence and chemoluminescence method,
And detection environment is required.Compared to fluorescence and chemoluminescence method, the biology biography based on localised plasmon resonance principle
Sensor has high throughput, and instrument needed for detection is simple, reliable results, it is cheap the advantages that.
Metal Nano structure has unique light, electricity, magnetic property, suitable for making LSPR (Localized Surface
Plasmon Resonance, localized surface plasmons resonance) biological sensing element.In Jie of nanostructured outer cladding sensitivity
Electric material can be used for detecting different kinds of molecules, therefore have potential application value in portable medical and field of fast detection.And
For nanostructured as LSPR sensing elements, the application for monitoring molecule or ion concentration in the body fluid in addition to blood rarely has report
Road.
The content of the invention
It is an object of the invention to provide a kind of sensor and its methods for making and using same for being used to detect body fluid pH.
The technical solution used in the present invention is：
A kind of sensor for being used to detect body fluid pH, including metal Nano structure substrate, metal Nano structure substrate outsourcing
It is wrapped with hydrogen ion concentration sensitive material.
Metal Nano structure substrate is wherein one in nano-metal particle substrate, nano metal cone oldered array substrate
Kind.
The thickness of hydrogen ion concentration sensitive material is 5nm~20 μm.
Hydrogen ion concentration sensitive material is at least one of polyaniline, polypyrrole layer, polypropylene acid layer.
A kind of preparation method of above-mentioned sensor for being used to detect body fluid pH, is by chemical oxidization method, in metal nano
Hydrogen ion concentration sensitive material is deposited in structural substrates.
In preparation method, chemical oxidization method is solution polymerization process or emulsion polymerization.
In preparation method, metal Nano structure substrate is obtained after optical clear substrate is immersed in nano-metal particle solution
Arrive, or nano metal cone oldered array is obtained after surfactant solution activation process.
A kind of integrated device for being used to detect body fluid pH, including a kind of above-mentioned sensor, hair for detecting body fluid pH
Optical diode light source and film crystal pipe detector.
A kind of method for detecting body fluid pH, is using a kind of above-mentioned integrated device detection body fluid for being used to detect body fluid pH
PH change.
Body fluid is the one of which in wound fluid, saliva, gastric juice.
The beneficial effects of the invention are as follows：
Biology sensor of the invention based on localised plasmon resonance principle is with high throughput, detecting instrument is simple, ties
The advantages that fruit is reliable and cheap, by the way that the material layer of hydrogen ion concentration sensitivity is coated on metal Nano structure, can use
In the quick detection and monitoring of human body fluid (such as wound fluid, saliva, gastric juice) pH.Sensor of the invention technology of preparing
Simply, it is of low cost and can integrate, suitable for producing in enormous quantities.
Brief description of the drawings
Fig. 1 is sensor construction schematic diagram made from embodiment 2；
Fig. 2 is the scanning electron microscope (SEM) photograph of the sensor surface of embodiment 2；
Fig. 3 is response diagram of the sensor to pH of embodiment 2.
Embodiment
A kind of sensor for being used to detect body fluid pH, including metal Nano structure substrate, metal Nano structure substrate outsourcing
It is wrapped with hydrogen ion concentration sensitive material.
Preferably, metal Nano structure substrate is nano-metal particle substrate, in nano metal cone oldered array substrate
One of which；It is further preferred that metal Nano structure substrate is nanogold particle substrate or aluminium nanocone oldered array substrate.
The thickness of hydrogen ion concentration sensitive material is 5nm~20 μm.
Preferably, hydrogen ion concentration sensitive material is at least one in polyaniline, polypyrrole layer, polypropylene acid layer
Kind；It is further preferred that hydrogen ion concentration sensitive material is polyaniline.
A kind of preparation method of above-mentioned sensor for being used to detect body fluid pH, is by chemical oxidization method, in metal nano
Hydrogen ion concentration sensitive material is deposited in structural substrates.
In preparation method, it is preferred that chemical oxidization method is solution polymerization process or emulsion polymerization.
Further, it is that metal Nano structure substrate is immersed in containing hydrogen when chemical oxidization method is solution polymerization process
Reacted in ion concentration sensitive material monomer, initiator and medium solution, the sensor for detecting body fluid pH is made.
Further, when chemical oxidization method is emulsion polymerization, be by metal Nano structure substrate be first immersed in containing
In the mixed liquor of hydrogen ion concentration sensitive material monomer and emulsifying agent, initiator for reaction is added, is made for detecting body fluid pH
Sensor.
Preferably, it is aniline containing hydrogen ion concentration sensitive material monomer；Medium solution is hydrochloric acid solution；Initiator was
Ammonium sulfate；Emulsifying agent is sodium dodecyl sulfate.
In preparation method, it is preferred that metal Nano structure substrate is that optical clear substrate is immersed in nano-metal particle
Obtained after solution, or nano metal cone oldered array is obtained after surfactant solution activation process.
Preferably, optical clear substrate is substrate of glass；Nano-metal particle solution is nanogold particle solution；Nanogold
It is aluminium nanocone oldered array to belong to cone oldered array；Surfactant is cetyl trimethyl ammonium.
Specifically, metal Nano structure substrate is by manufactured after glass matrix immersion nanogold particle solution, it is described
Nanogold particle be by made of the method for citrate reduction gold chloride；Alternatively, metal Nano structure substrate is to pass through
Made of after the immersion cetyl trimethyl ammonium solution activation of aluminium nanocone oldered array.
A kind of integrated device for being used to detect body fluid pH, including a kind of above-mentioned sensor, hair for detecting body fluid pH
Optical diode light source and film crystal pipe detector.
Further, sensor, LED source and film crystal pipe detector are integrated for detecting body
The integrated device of liquid pH.
A kind of method for detecting body fluid pH, is using a kind of above-mentioned integrated device detection body fluid for being used to detect body fluid pH
PH change.
Body fluid is the one of which in wound fluid, saliva, gastric juice.
The principle of pH sensings of the present invention is as follows：The material for being wrapped in metallic nanostructured surface has sensitivity to hydrogen ion
Property, or have response to the doping level of proton.When the pH value of surrounding environment changes, hydrogen ion sensitive material can be due to
Proton doping level is different and the change on electricity structure occurs, and this change will influence the folding on nano structural material surface
Rate is penetrated, and then influences the LSPR effects of nanostructured.The influence of this LSPR effects not only shows as LSPR peaks on collection of illustrative plates
Red shift or blue shift, and with the change of macroscopic solution colour.And the usually this proton to hydrogen ion sensitive material is mixed
Miscellaneous is completely reversibility, therefore the response of the metal Nano structure composite material can also have preferable invertibity and reappearance.
Present disclosure is described in further detail below by way of specific embodiment.
Embodiment 1：
For the sensor of embodiment 1 using aluminium (Al) nanocone structures array as element, polyaniline is sensitive material, specific system
Preparation Method is as follows：
Step 1：First by clean Al nano-cone arrays be immersed in cetyl trimethyl ammonium (CTAB solution,
In 0.5M), 5min is stood.
Step 2：Then Al nano-cone arrays are taken out, after being cleaned with pure water, is put into aniline (2mM, 1.5mL) and 12
In sodium alkyl sulfate (SDS, 40mM, 0.25mL) mixed solution, 2min is fully vibrated；
Step 3：Secondly, hydrochloric acid solution (the 2mM ammonium persulfates/10mM salt containing ammonium persulfate are added in the solution
Acid, 1.5mL), vibration is uniformly mixed solution；
Step 4：Reaction proceeds by after standing.After standing 3~24h, Al nano-cone arrays are taken out from solution, are used
The clean array surface of pure water rinsing is that can obtain the Al nano-cone arrays of polyaniline in surface modification.
Step 5：The polyaniline thickness on Al nano-cone arrays surface can be adjusted by controlling the reaction time：During reaction
Between it is longer, the thickness of polyaniline is thicker.After 24h, react basic and stop.At this time, repeating above step can further increase
The thickness of addition polymerization aniline parcel, the thickness section of polyaniline parcel are advisable with 5nm~20 μm.
Embodiment 2：
For the sensor of embodiment 2 using glass as base material, gold nano grain is element, and polyaniline is sensitive material, its
The structure of the visible attached drawing 1 of structure is schematic diagram.In Fig. 1,1- substrate of glass；2- gold nano grains；3- polyanilines.Specific system
Preparation Method is as follows：
Step 1：Substrate preparation：Substrate of glass is cleaned, hydrophilicity-imparting treatment.
Step 2：The synthesis of gold nano grain：The water of addition 20mL, which is put into -4 DEG C of refrigerator, in beaker A cools down；Again
Separately a beaker B is taken to add ultra-pure water, the sodium citrate solution of 1mL and the chlorauric acid solution of 1mL inward；Weigh the boron of 0.038g
Sodium hydride is placed in beaker C, takes the frozen water of 10mL to be added in beaker C from beaker A；Then solvent sodium borohydride solution is taken
1mL is added in beaker B and rocks at once.Beaker B need to be encased with tinfoil does lucifuge processing, when standing 4 is small, can obtain required
Seed solution.1.83g CTAB are taken after adding the water of 100mL, to be placed in supersonic cleaning machine in beaker D and accelerate CTAB
Dissolving；Solvent CTAB is poured into beaker E, F, G respectively, adds suitable sodium hydroxide solution, Vitamin C inward respectively
Acid solution and chlorauric acid solution；Take the seed solution synthesized by 1mL to be added in beaker E and rock rapidly, take 5mL immediately after
Solution in E is added in beaker F and rocks rapidly；Take the solution in the beaker F of 2.5mL to be added in beaker G again, then will
Stand more than 7h after beaker G sealings, after then adding sodium chloride solution inward, stand 12 it is small when after, gold nano grain will be
Beaker bottom deposits.
Step 3：The making of gold nano grain substrate：The nano particle that the step of taking 3mL two synthesizes is centrifuged and is resuspended 2 times
Obtain nanogold particle solution；The substrate of glass for the hydrophilicity-imparting treatment that step 1 is obtained is soaked in 24 in gold nano grain solution
Hour, obtain gold nano grain substrate.
Step 4：Polyaniline is wrapped up in gold nano grain substrate surface：(aniline monomer concentration is configuration mixed liquor
2.0mM, ammonium persulfate concentrations 2mM, surfactant SDS concentration are 40mM), pH is adjusted in 2.0, by step with concentrated hydrochloric acid
Three obtained nanogold particle substrates are soaked in the mixing liquid of beaker；6 it is small when after, solution in beaker is changed into blackish green
Color, takes out slide rinse repeatedly, removes the polyaniline and ammonium persulfate of its surface excess；Then it is being baked to, drying is completed
Nanogold-polyaniline composite particles sensor is just obtained afterwards.
Attached drawing 2 is the scanning electron microscope (SEM) photograph of the sensor surface of embodiment 2.As it is clear from fig. 2 that polyaniline covering is uniform, and
Gold nano structure has been wrapped up, effectively forms sensitive layer.Attached drawing 3 is response diagram of the sensor to pH of embodiment 2.Can from Fig. 3
See, the sensor of embodiment 2 has good response effect in the different sections that different pH are 3~5；In pH 4.0, photoresponse is strong
Degree keeps basicly stable.
Sensor made from embodiment 2 and LED source, film crystal pipe detector are integrated for examining
Survey the integrated device of body fluid pH.This integrated device is placed in the facing in oral cavity, detects the signal strength and letter of thin film transistor (TFT)
Number Strength Changes can detection mouth intracavitary saliva pH changes.
Claims (10)
- A kind of 1. sensor for being used to detect body fluid pH, it is characterised in that：Including metal Nano structure substrate, metal Nano structure Substrate is wrapped with hydrogen ion concentration sensitive material.
- A kind of 2. sensor for being used to detect body fluid pH according to claim 1, it is characterised in that：Metal Nano structure base Bottom is nano-metal particle substrate, the one of which in nano metal cone oldered array substrate.
- A kind of 3. sensor for being used to detect body fluid pH according to claim 1, it is characterised in that：Hydrogen ion concentration is sensitive The thickness of material layer is 5nm~20 μm.
- A kind of 4. sensor for being used to detect body fluid pH according to claim 3, it is characterised in that：Hydrogen ion concentration is sensitive Material layer is at least one of polyaniline, polypyrrole layer, polypropylene acid layer.
- 5. a kind of preparation method for the sensor for being used to detect body fluid pH of Claims 1 to 4 any one of them, its feature exist In：By chemical oxidization method, hydrogen ion concentration sensitive material is deposited in metal Nano structure substrate.
- A kind of 6. preparation method of sensor for being used to detect body fluid pH according to claim 5, it is characterised in that：Chemistry Oxidizing process is solution polymerization process or emulsion polymerization.
- A kind of 7. preparation method of sensor for being used to detect body fluid pH according to claim 5, it is characterised in that：Metal Nanostructured substrate obtains after optical clear substrate is immersed in nano-metal particle solution, or nano metal is bored What oldered array obtained after surfactant solution activation process.
- A kind of 8. integrated device for being used to detect body fluid pH, it is characterised in that：Passed including Claims 1 to 4 any one of them Sensor, LED source and film crystal pipe detector.
- A kind of 9. method for detecting body fluid pH, it is characterised in that：The pH of integrated device detection body fluid described in usage right requirement 8 Change.
- A kind of 10. method for detecting body fluid pH according to claim 9, it is characterised in that：Body fluid is wound fluid, saliva One of which in liquid, gastric juice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710943779.4A CN107941778A (en) | 2017-10-11 | 2017-10-11 | A kind of sensor and its methods for making and using same for being used to detect body fluid pH |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710943779.4A CN107941778A (en) | 2017-10-11 | 2017-10-11 | A kind of sensor and its methods for making and using same for being used to detect body fluid pH |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107941778A true CN107941778A (en) | 2018-04-20 |
Family
ID=61935173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710943779.4A Pending CN107941778A (en) | 2017-10-11 | 2017-10-11 | A kind of sensor and its methods for making and using same for being used to detect body fluid pH |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107941778A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109700600A (en) * | 2018-12-18 | 2019-05-03 | 中国人民解放军第四军医大学 | PH sensor, sensing dressing and manufacture, building method comprising the sensor |
| CN110186875A (en) * | 2019-05-21 | 2019-08-30 | 天津大学 | Surface plasmon resonance optical-fiber type pH value measurement method and sensor |
| CN110373708A (en) * | 2019-07-31 | 2019-10-25 | 东南大学 | Adjustable concentration realizes that the nanometer pinpoint of needle point cone angle control prepares platform and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800831A (en) * | 2004-12-17 | 2006-07-12 | 安捷伦科技有限公司 | Sensor having integrated light detector and/or light source |
| CN1877310A (en) * | 2005-06-09 | 2006-12-13 | 阿瓦戈科技通用Ip(新加坡)股份有限公司 | Analyte detection using luminescence concentration |
| CN102083741A (en) * | 2008-07-03 | 2011-06-01 | 浦项工科大学校产学协力团 | pH sensitive metal nanoparticle and preparation method |
| CN105510388A (en) * | 2015-11-25 | 2016-04-20 | 中国科学院电工研究所 | Wearable apparatus for detecting pH value of sweat |
| CN104458848B (en) * | 2014-12-05 | 2017-01-25 | 浙江大学 | Comb nanosensor with pH indication and self-calibration and preparation method of comb nanosensor |
| CN106563133A (en) * | 2016-11-21 | 2017-04-19 | 中山大学 | Gold nano-composite particles for measuring three-dimensional distribution of pH and preparation method and application thereof |
-
2017
- 2017-10-11 CN CN201710943779.4A patent/CN107941778A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800831A (en) * | 2004-12-17 | 2006-07-12 | 安捷伦科技有限公司 | Sensor having integrated light detector and/or light source |
| CN1877310A (en) * | 2005-06-09 | 2006-12-13 | 阿瓦戈科技通用Ip(新加坡)股份有限公司 | Analyte detection using luminescence concentration |
| CN102083741A (en) * | 2008-07-03 | 2011-06-01 | 浦项工科大学校产学协力团 | pH sensitive metal nanoparticle and preparation method |
| CN104458848B (en) * | 2014-12-05 | 2017-01-25 | 浙江大学 | Comb nanosensor with pH indication and self-calibration and preparation method of comb nanosensor |
| CN105510388A (en) * | 2015-11-25 | 2016-04-20 | 中国科学院电工研究所 | Wearable apparatus for detecting pH value of sweat |
| CN106563133A (en) * | 2016-11-21 | 2017-04-19 | 中山大学 | Gold nano-composite particles for measuring three-dimensional distribution of pH and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| GABI DROCHIOIU: "Chronic metabolic acidosis may be the cause of cachexia: Body fluid pH correction may be an effective therapy", 《MEDICAL HYPOTHESES》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109700600A (en) * | 2018-12-18 | 2019-05-03 | 中国人民解放军第四军医大学 | PH sensor, sensing dressing and manufacture, building method comprising the sensor |
| CN110186875A (en) * | 2019-05-21 | 2019-08-30 | 天津大学 | Surface plasmon resonance optical-fiber type pH value measurement method and sensor |
| CN110373708A (en) * | 2019-07-31 | 2019-10-25 | 东南大学 | Adjustable concentration realizes that the nanometer pinpoint of needle point cone angle control prepares platform and method |
| CN110373708B (en) * | 2019-07-31 | 2021-04-30 | 东南大学 | Nano needle tip preparation platform and method for adjusting concentration to realize needle tip cone angle control |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Dual-functional ultrathin wearable 3D particle-in-cavity SF-AAO-Au SERS sensors for effective sweat glucose and lab-on-glove pesticide detection | |
| Liu et al. | SERS substrate fabrication for biochemical sensing: Towards point-of-care diagnostics | |
| Mani et al. | Microneedle pH sensor: direct, label-free, real-time detection of cerebrospinal fluid and bladder pH | |
| El-Said et al. | Monitoring in vitro neural stem cell differentiation based on surface-enhanced Raman spectroscopy using a gold nanostar array | |
| Kant et al. | Xanthine oxidase functionalized Ta2O5 nanostructures as a novel scaffold for highly sensitive SPR based fiber optic xanthine sensor | |
| CN107941778A (en) | A kind of sensor and its methods for making and using same for being used to detect body fluid pH | |
| Li et al. | Affinity based glucose measurement using fiber optic surface plasmon resonance sensor with surface modification by borate polymer | |
| Hussain et al. | Sweat-based noninvasive skin-patchable urea biosensors with photonic interpenetrating polymer network films integrated into PDMS chips | |
| Zhao et al. | Core-shell structured gold nanorods on thread-embroidered fabric-based microfluidic device for Ex Situ detection of glucose and lactate in sweat | |
| US9921163B2 (en) | Method and device for detecting analytes | |
| Usha et al. | FO-SPR based dextrose sensor using Ag/ZnO nanorods/GOx for insulinoma detection | |
| Prakash et al. | Silk fibroin as an immobilization matrix for sensing applications | |
| Liu et al. | SERS paper slip based on 3D dendritic gold nanomaterials coupling with urchin-like nanoparticles for rapid detection of thiram | |
| CN107828772A (en) | A kind of immobilized enzyme reactor for ratio fluorescent detection and preparation method thereof | |
| Kant et al. | A highly sensitive and distinctly selective D-sorbitol biosensor using SDH enzyme entrapped Ta2O5 nanoflowers assembly coupled with fiber optic SPR | |
| Huang et al. | Temperature controlling fiber optic glucose sensor based on hydrogel-immobilized GOD complex | |
| Hu et al. | Multiplex chroma response wearable hydrogel patch: visual monitoring of urea in body fluids for health prognosis | |
| CN107880872A (en) | A kind of surface-enhanced Raman fluorescent dual module nano-probe based on conjugated polymer and preparation method thereof | |
| CN112957038B (en) | Preparation method of high-sensitivity self-cleaning type blood oxygen sensor based on photonic crystal fluorescence enhancement | |
| Hou et al. | Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite | |
| Ma et al. | Transparent and flexible AuNSs/PDMS-based SERS substrates for in-situ detection of pesticide residues | |
| Theerasilp et al. | Halochromic polymer nanosensors for simple visual detection of local pH in coatings | |
| JP6980195B2 (en) | Equipment and methods for detecting an analyte using heat waves. | |
| Basu et al. | Multi-parametric optical glucose sensor based on surface functionalized nano-porous silicon | |
| Choi et al. | A recyclable CNC-milled microfluidic platform for colorimetric assays and label-free aged-related macular degeneration detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180420 |
|
| RJ01 | Rejection of invention patent application after publication |