CN107941678B - Myocardial cell detection method based on non-close-packed photonic crystal film and application thereof - Google Patents
Myocardial cell detection method based on non-close-packed photonic crystal film and application thereof Download PDFInfo
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Abstract
The invention discloses a myocardial cell detection method based on a non-close-packed photonic crystal film and application thereof, wherein the detection method comprises the following steps: 1) preparing a non-close-packed photonic crystal film; 2) culturing the myocardial cells based on the non-close-packed photonic crystal film; 3) detecting the myocardial cells; 4) and (6) analyzing the data. The method can sensitively and efficiently detect the contraction and beating frequency of the cardiac muscle cells by means of the optical sensing property of the non-close-packed photonic crystal film, has the advantages of low cost and simple process, has the internal advantages of stability, sensitivity and efficiency in cardiac muscle cell detection and screening and evaluation of cardiac drugs, and can be used for screening and evaluation of the cardiac drugs.
Description
Technical Field
The invention relates to a myocardial cell detection method based on a non-close-packed photonic crystal film and application thereof, belonging to the technical field of biomedical material research.
Background
The heart is one of the most important organs in the human body, and the main functions of the heart are to promote blood flow, provide sufficient blood flow to organs and tissues, supply oxygen and various nutrients, remove metabolic wastes, and maintain normal metabolism and functions of other organs and tissue cells. In recent years, with the increasing threat of coronary heart disease and congenital heart disease to human beings, the research, development, screening and evaluation of effective heart drugs become one of the problems to be solved urgently in the biomedical field.
At present, for the development of human drugs, the effect of the drug on a target object and the influence of the drug on normal physiological structures of other cells, tissues, organs and the like are mainly detected through in vitro cell culture and in vivo animal experiments, so that the drug effect is evaluated. However, in vitro cell culture tends to be planar two-dimensional culture, and the mode cannot truly and effectively simulate in vivo microenvironment and complex physiological processes among tissues and organs; in vivo animal experiments also have limitations in the uncertainty of constructing animal models, differences in different animal species, and some humanistic factors, and therefore constructing an in vitro model to simulate physiological processes, perform drug screening, and evaluation at the cellular or tissue level has attracted extensive attention. In the research of heart and myocardial cells, the contraction and beating frequency of the myocardial cells are extremely important evaluation parameters and are important indexes for evaluating the activity of the myocardial cells; however, constructing a heart chip requires a complex detection system, often relying on large instrumentation, and lacks intuitiveness. Therefore, designing and developing sensing materials with high sensitivity of cardiomyocyte contraction and beating frequency, and accordingly constructing a stable cardiac drug evaluation platform still faces this serious challenge.
Disclosure of Invention
The technical problem is as follows: the invention aims to provide a myocardial cell detection method based on a non-close-packed photonic crystal film and application thereof, wherein the non-close-packed photonic crystal film in the detection method provides a three-dimensional growth structure for myocardial cells and provides stable and efficient optical sensing signals for myocardial cell contraction and beating frequencies.
The technical scheme is as follows: the invention provides a myocardial cell detection method based on a non-close-packed photonic crystal film, which comprises the following steps:
1) preparing a non-close-packed photonic crystal film: preparing a non-close-packed photonic crystal film by using the colloidal particles with charged surfaces and the biocompatible hydrogel prepolymer solution by adopting a colloidal particle self-assembly method;
2) myocardial cell culture based on non-close-packed photonic crystal films: planting myocardial cells on the surface of the non-close-packed photonic crystal film, and culturing to obtain the non-close-packed photonic crystal film with the myocardial cells;
3) and (3) detecting the myocardial cells: the characteristic reflection peak periodic variation data of the non-close-packed photonic crystal film is collected, wherein the characteristic reflection peak periodic variation data is caused by the contraction and relaxation of the myocardial cells on the non-close-packed photonic crystal film;
4) and (3) data analysis: analyzing the characteristic reflection peak periodic variation data of the non-close-packed photonic crystal film collected in the step 3) to obtain the contraction force and beating frequency conditions of the myocardial cells.
Wherein:
the non-close-packed photonic crystal film comprises the following specific steps:
① dispersing surface charged colloidal particles into a biocompatible hydrogel prepolymer solution by using a colloidal particle self-assembly method, wherein the concentration of the biocompatible hydrogel prepolymer is 5-20 wt%, then adding 2-hydroxy-2-methyl-1-phenyl-1-acetone, the volume of the added biocompatible hydrogel prepolymer solution is 0.5-5% of the volume of the biocompatible hydrogel prepolymer solution, fully mixing uniformly, adding ion exchange resin with the total volume of 10-30%, and fully oscillating to form a colloidal crystal solution;
② the colloidal crystal solution obtained in step ① is injected into a mold and cured to form a planar or patterned non-close-packed photonic crystal film.
The biocompatible hydrogel precursor in the step 1) is one of polyacrylamide, poly-N-isopropylacrylamide, methacrylate gelatin, four-arm-polyethylene glycol or polyethylene glycol diacrylate.
The surface-charged colloidal particles are one of surface-charged silicon dioxide, polystyrene or titanium dioxide spherical particles, and the particle size of the surface-charged colloidal particles is 50-150 nm.
The specific process of culturing the myocardial cells based on the non-close-packed photonic crystal film is to plant the myocardial cells on the surface of the non-close-packed photonic crystal film at 36-40 ℃ and 2-10 wt% of CO2Culturing for 2-10 days in a culture environment to obtain the non-close-packed photonic crystal film with the myocardial cells.
And 3) the device for acquiring the periodic variation data of the characteristic reflection peak of the non-close-packed photonic crystal film is a spectrometer or a spectrometer.
And 4) the calculation formula of the myocardial beating frequency in the step 4) is that f is 1/t, wherein f is the myocardial beating frequency, and t is the single-cycle change time of the characteristic reflection peak.
The non-close-packed photonic crystal film in the step 1) has structural color, the structural color covers the visible light range, and the reflection peak position is between 400nm and 700 nm.
The curing process described in step ② is ultraviolet curing.
The myocardial cells are various myocardial cells obtained by different species and induced differentiation.
The reflection peak of the non-close-packed photonic crystal film in the step 1) is adjusted by adjusting the size of the surface-charged colloidal particles or the distance between the surface-charged colloidal particles.
When the myocardial cells grow on the surface of the non-close-packed photonic crystal film and shrink regularly, the structural color of the non-close-packed photonic crystal film changes regularly and periodically, and the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity are detected by using an epitaxial equipment spectrometer or a spectrometer; drawing a myocardial contraction force-characteristic reflection peak standard curve, calculating myocardial contraction force according to the curve and the change of the position and the intensity of the measured characteristic reflection peak, and calculating myocardial beating frequency through single periodic change of the characteristic reflection peak.
The invention also provides application of the myocardial cell detection method based on the non-close-packed photonic crystal film, and the method is applied to screening and evaluation of heart drugs. Based on the sensitive and efficient optical sensing property of the non-close-packed photonic crystal film, the contraction and beating frequency of the myocardial cells can be changed in the process of the action of different drugs on the myocardial cells, and the drugs are screened and evaluated by detecting the color change and the shape change of the non-close-packed photonic crystal film.
The method can be applied to detecting the influence of physical electrical stimulation, chemical ions or biological factors on the contraction and beating frequency of the myocardial cells, and can also be applied to the influence of myocardial medicaments of isoproterenol and digoxin, electrical stimulation and calcium ions on the contraction and beating frequency of the myocardial cells.
Has the advantages that: compared with the prior art, the invention has the following advantages:
1) in the myocardial cell detection method based on the non-close-packed photonic crystal film, the non-close-packed photonic crystal film not only provides a carrier with good biocompatibility and a three-dimensional growth space for the growth of myocardial cells, but also provides stable optical sensing signals for detecting the contractility and the beating frequency of the myocardial cells due to the stable optical property and the sensitive and efficient optical sensing characteristic;
2) compared with other detection methods, the method does not need to rely on large-scale instruments or complex detection systems, can macroscopically observe the detection result by naked eyes, has no influence on the detected system in the aspects of physiological pharmacology and the like, and has certain resistance to external environment and interference factors such as physics, chemistry and the like;
3) the detection of the contraction and beating frequency of the myocardial cells based on the non-close-packed photonic crystal film and the screening and evaluation of the heart drugs can show the advantages of stability, sensitivity and high efficiency;
4) the non-close-packed photonic crystal film provided by the invention has the characteristics of simple preparation method, low cost, no dependence on large instruments and equipment and the like, and has wide application prospect in the field of biomedical materials such as screening and evaluation of heart drugs.
Drawings
FIG. 1 is a schematic diagram of the preparation of a non-close-packed photonic crystal film;
FIG. 2 is a schematic diagram of the growth of cardiomyocytes on the surface of a non-close-packed photonic crystal thin film.
Detailed Description
The above-described scheme is further illustrated below with reference to specific examples. It should be understood that these examples are for illustrative purposes and are not intended to limit the scope of the present invention. The conditions used in the examples may be further adjusted according to the conditions of the particular manufacturer, and the conditions not specified are generally the conditions in routine experiments.
The method utilizes the unique optical characteristics and efficient sensing attributes of the non-close-packed photonic crystal film to perform the visual sensing detection of the myocardial cell contraction and beating frequency according to the characteristic reflection peak (namely color) of the non-close-packed photonic crystal film, when the myocardial cell grows on the surface of the non-close-packed photonic crystal film and generates regular contraction, the microscopic change of the particle spacing of the non-close-packed photonic crystal film or the macroscopic change of the angle and the color of the non-close-packed photonic crystal film is caused, and the regular periodic change of the structural color of the non-close-packed photonic crystal film is utilized to reflect the myocardial cell contraction and beating frequency; the non-close-packed photonic crystal film has certain environmental resistance, the structural color of the non-close-packed photonic crystal film is slightly interfered by the outside, and the color change is mainly caused by the contraction of the myocardial cells growing on the non-close-packed photonic crystal film.
Example 1
1. Preparation of non-close-packed photonic crystal film
Preparing a non-close-packed photonic crystal film by using a colloidal particle self-assembly method and using a silicon dioxide ordered structure and biocompatible hydrogel:
1) dispersing high-charged silicon dioxide particles with the diameter of 100nm into a polyacrylamide solution with the concentration of 15 wt%, then adding 2-hydroxy-2-methyl-1-phenyl-1-acetone (HMPP) with the volume of 3% of the mixed solution, fully mixing uniformly, adding ion exchange resin with the total volume of 10%, fully oscillating and purifying the charge of the colloidal crystal solution to form a colloidal crystal solution;
2) and injecting the colloidal crystal solution between the two glass slides, forming a non-close-packed structure by self-assembly of silicon dioxide particles, and curing by ultraviolet light to obtain the polyacrylamide non-close-packed photonic crystal film.
2. The non-close-packed photonic crystal film is used for culturing the myocardial cells: planting the extracted SD milk rat myocardial cells on the surface of the prepared acrylamide non-close-packed photonic crystal film, and culturing at 37 ℃ and 5% CO2Culturing in culture environment for 2 days.
3. And (3) detecting the myocardial cells: when the myocardial cells growing on the surface of the polyacrylamide non-close-packed photonic crystal film after 2 nutrient cycles shrink regularly, the structural color of the photonic crystal film changes regularly and periodically, and the shift of the characteristic reflection peak position and the periodic change of the intensity of the non-close-packed photonic crystal film are detected by an epitaxial equipment spectrometer or a spectrometer.
4. And (3) data analysis: drawing a standard curve of myocardial cell contraction force-characteristic reflection peak by using the measured data of the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity; calculating myocardial contraction force according to the deviation of the curve and the measured position of the characteristic reflection peak and the periodic change of the strength of the characteristic reflection peak, and calculating myocardial beating frequency according to the single periodic change of the characteristic reflection peak:
the contractility of the myocardial cells is determined according to the characteristic reflection peak offset delta lambda of the non-close-packed photonic crystal thin film, under the same culture condition, different stimuli are applied to the myocardial cells, and the larger the delta lambda offset is, the larger the contractility of the myocardial cells is.
The calculation formula of the myocardial beating frequency is that f is 1/t, wherein f is the myocardial beating frequency, and t is the single cycle change time of the characteristic reflection peak.
5. Screening and evaluation of cardiac drugs based on acrylamide non-close-packed photonic crystal film
The growth environment of the myocardial cells growing on the surface of the acrylamide non-close-packed photonic crystal film is changed, for example, after factors such as isoproterenol, digoxin, electrical stimulation, calcium ion influence and the like are added, the contraction of the myocardial cells is changed, the change of the color and the shape of the polyacrylamide non-close-packed photonic crystal film is caused, and the evaluation of the influence of the factors on the myocardial cells is realized through the detection of the color and the spectrum change of the polyacrylamide non-close-packed photonic crystal film.
Example 2
1. Preparation of non-close-packed photonic crystal film
Preparing a non-close-packed photonic crystal film by using a colloidal particle self-assembly method and utilizing an ordered structure of polystyrene charged spherical particles and biocompatible hydrogel:
1) dispersing high-charged polystyrene particles with the diameter of 50nm into poly (N-isopropylacrylamide) solution with the concentration of 5 wt%, then adding 2-hydroxy-2-methyl-1-phenyl-1-acetone (HMPP) with the volume of 5% of the volume of the mixed solution, fully and uniformly mixing, adding ion exchange resin with the total volume of 30%, fully oscillating and purifying the charge of the colloidal crystal solution to form a colloidal crystal solution;
2) and injecting the colloidal crystal solution between two glass slides, self-assembling polystyrene particles to form a non-close-packed structure, and curing by ultraviolet light to obtain the poly N-isopropylacrylamide non-close-packed photonic crystal film.
2. The non-close-packed photonic crystal film is used for culturing the myocardial cells: planting the extracted SD milk rat myocardial cells on the surface of the prepared acrylamide non-close-packed photonic crystal film, and culturing at 36 ℃ and 2% CO2Culturing in culture environment for 10 days.
3. And (3) detecting the myocardial cells: when the myocardial cells growing on the surface of the poly N-isopropylacrylamide non-close-packed photonic crystal film generate regular contraction after 10 nutrient cycles, the characteristic reflection peak of the photonic crystal film generates regular periodic change, and the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity are detected by an epitaxial equipment spectrometer or a spectrometer.
4. And (3) data analysis: drawing a standard curve of myocardial cell contraction force-characteristic reflection peak by using the measured data of the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity; calculating myocardial contraction force according to the deviation of the curve and the measured position of the characteristic reflection peak and the periodic change of the strength of the characteristic reflection peak, and calculating myocardial beating frequency according to the single periodic change of the characteristic reflection peak:
the contractility of the myocardial cells is determined according to the characteristic reflection peak offset delta lambda of the non-close-packed photonic crystal thin film, under the same culture condition, different stimuli are applied to the myocardial cells, and the larger the delta lambda offset is, the larger the contractility of the myocardial cells is.
The calculation formula of the myocardial beating frequency is that f is 1/t, wherein f is the myocardial beating frequency, and t is the single cycle change time of the characteristic reflection peak.
5. Screening and evaluation of cardiac drugs of poly N-isopropylacrylamide non-close-packed photonic crystal film
The growth environment of the myocardial cells growing on the surface of the poly-N-isopropylacrylamide non-close-packed photonic crystal film is changed, for example, after isoproterenol, digoxin, electrical stimulation, calcium ion influence and other factors are added, the contraction of the myocardial cells is changed, the color and the shape of the poly-N-isopropylacrylamide non-close-packed photonic crystal film are changed, and the influence of the factors on the myocardial cells is evaluated by detecting the color and the spectrum change of the poly-N-isopropylacrylamide non-close-packed photonic crystal film.
Example 3
1. Preparation of non-close-packed photonic crystal film
Preparing a non-close-packed photonic crystal film by adopting a colloid particle self-assembly method and utilizing an ordered structure of charged spherical titanium dioxide particles and biocompatible hydrogel:
1) dispersing high-charged titanium dioxide particles with the diameter of 150nm into a methacrylate gelatin solution with the concentration of 20 wt%, then adding 2-hydroxy-2-methyl-1-phenyl-1-acetone (HMPP) with the volume of 0.5% of the mixed solution, fully and uniformly mixing, adding ion exchange resin with the total volume of 20%, fully oscillating and purifying the charge of the colloidal crystal solution to form a colloidal crystal solution;
2) and injecting the colloidal crystal solution between the two glass slides, carrying out self-assembly on titanium dioxide particles to form a non-close-packed structure, and carrying out ultraviolet light curing to obtain the methacrylate gelatin non-close-packed photonic crystal film.
2. The non-close-packed photonic crystal film is used for culturing the myocardial cells: planting freshly extracted SD milk rat myocardial cells on the surface of the prepared methacrylate gelatin non-close-packed photonic crystal film, and culturing at 40 ℃ and 10% CO2Culturing in culture environment for 5 days.
3. And (3) detecting the myocardial cells: when the myocardial cells growing on the surface of the methacrylate gelatin non-close-packed photonic crystal film generate regular shrinkage after a culture period of 5 days, the characteristic reflection peak of the photonic crystal film generates regular periodic change, and the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity are detected by using an epitaxial equipment spectrometer or a spectrometer.
4. And (3) data analysis: drawing a standard curve of myocardial cell contraction force-characteristic reflection peak by using the measured data of the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity; calculating myocardial contraction force according to the deviation of the curve and the measured position of the characteristic reflection peak and the periodic change of the strength of the characteristic reflection peak, and calculating myocardial beating frequency according to the single periodic change of the characteristic reflection peak:
the contractility of the myocardial cells is determined according to the characteristic reflection peak offset delta lambda of the non-close-packed photonic crystal thin film, under the same culture condition, different stimuli are applied to the myocardial cells, and the larger the delta lambda offset is, the larger the contractility of the myocardial cells is.
The calculation formula of the myocardial beating frequency is that f is 1/t, wherein f is the myocardial beating frequency, and t is the single cycle change time of the characteristic reflection peak.
5. Screening evaluation of heart drugs based on methacrylate gelatin non-close-packed photonic crystal thin film
The growth environment of the myocardial cells growing on the surface of the methacrylate gelatin non-close-packed photonic crystal film is changed, for example, after factors such as isoproterenol, digoxin, electrical stimulation, calcium ion influence and the like are added, the contraction of the myocardial cells is changed, the change of the color and the shape of the methacrylate gelatin non-close-packed photonic crystal film is caused, and the influence of the factors on the myocardial cells is evaluated by detecting the color and the spectrum change of the methacrylate gelatin non-close-packed photonic crystal film.
Example 4
1. Preparation of non-close-packed photonic crystal film
Preparing a non-close-packed photonic crystal film by using a colloidal particle self-assembly method and utilizing a silicon dioxide charged spherical particle ordered structure and biocompatible hydrogel:
1) dispersing high-charged silica particles with the diameter of 150nm into a polyethylene glycol diacrylate (PEGDA) solution with the concentration of 10%, then adding 2-hydroxy-2-methyl-1-phenyl-1-acetone (HMPP) with the volume of 1% of the solution, fully and uniformly mixing, adding ion exchange resin with the total volume of 15%, fully oscillating the charges of the purified colloidal crystal solution, and forming a colloidal crystal solution;
2) and injecting the colloidal crystal solution between the two glass slides, forming a non-close-packed structure by self-assembly of silicon dioxide particles, and curing by ultraviolet light to obtain the PEGDA non-close-packed photonic crystal film.
2. The non-close-packed photonic crystal film is used for culturing the myocardial cells: planting freshly extracted SD rat myocardial cells on the surface of the prepared PEGDA non-close-packed photonic crystal film, and culturing at 38 ℃ and 8% CO2Culturing in culture environment for 8 days.
3. And (3) detecting the myocardial cells: when the myocardial cells growing on the surface of the PEGDA non-close-packed photonic crystal film generate regular contraction after 8 days of culture period, the spectrum of the photonic crystal film generates regular periodic change, and the shift of the characteristic reflection peak position and the periodic change of the intensity of the non-close-packed photonic crystal film are detected by an epitaxial equipment spectrometer or a spectrometer.
4. And (3) data analysis: drawing a standard curve of myocardial cell contraction force-characteristic reflection peak by using the measured data of the shift of the position of the characteristic reflection peak of the non-close-packed photonic crystal film and the periodic change of the intensity; calculating myocardial contraction force according to the deviation of the curve and the measured position of the characteristic reflection peak and the periodic change of the strength of the characteristic reflection peak, and calculating myocardial beating frequency according to the single periodic change of the characteristic reflection peak:
the contractility of the myocardial cells is determined according to the characteristic reflection peak offset delta lambda of the non-close-packed photonic crystal thin film, under the same culture condition, different stimuli are applied to the myocardial cells, and the larger the delta lambda offset is, the larger the contractility of the myocardial cells is.
The calculation formula of the myocardial beating frequency is that f is 1/t, wherein f is the myocardial beating frequency, and t is the single cycle change time of the characteristic reflection peak.
5. Heart drug screening and evaluating method based on PEGDA non-close-packed photonic crystal film
The growth environment of the myocardial cells growing on the surface of the PEGDA non-close-packed photonic crystal film is changed, for example, after isoproterenol, digoxin, electrical stimulation, calcium ion influence and other factors are added, the contraction of the myocardial cells is changed, the change of the color and the shape of the PEGDA non-close-packed photonic crystal film is caused, and the influence of the factors on the myocardial cells is evaluated by detecting the color and the spectrum change of the PEGDA non-close-packed photonic crystal film.
Claims (7)
1. A myocardial cell detection method based on a non-close-packed photonic crystal film is characterized by comprising the following steps: the method comprises the following steps:
1) preparing a non-close-packed photonic crystal film: preparing a non-close-packed photonic crystal film by using the colloidal particles with charged surfaces and the biocompatible hydrogel prepolymer solution by adopting a colloidal particle self-assembly method;
2) myocardial cell culture based on non-close-packed photonic crystal films: planting myocardial cells on the surface of the non-close-packed photonic crystal film, and culturing to obtain the non-close-packed photonic crystal film with the myocardial cells;
3) and (3) detecting the myocardial cells: the characteristic reflection peak periodic variation data of the non-close-packed photonic crystal film is collected, wherein the characteristic reflection peak periodic variation data is caused by the contraction and relaxation of the myocardial cells on the non-close-packed photonic crystal film;
4) and (3) data analysis: analyzing the characteristic reflection peak periodic variation data of the non-close-packed photonic crystal film collected in the step 3) to obtain the contraction force and beating frequency conditions of the myocardial cells;
the preparation method of the non-close-packed photonic crystal film comprises the following specific steps:
① dispersing surface charged colloidal particles into a biocompatible hydrogel prepolymer solution by using a colloidal particle self-assembly method, wherein the concentration of the biocompatible hydrogel prepolymer is 5-20 wt%, then adding 2-hydroxy-2-methyl-1-phenyl-1-acetone, the volume of the added biocompatible hydrogel prepolymer solution is 0.5-5% of the volume of the biocompatible hydrogel prepolymer solution, fully mixing uniformly, adding ion exchange resin with the total volume of 10-30%, and fully oscillating to form a colloidal crystal solution;
②, injecting the colloidal crystal solution obtained in step ① into a mould, and curing to form a planar non-close-packed photonic crystal film or a patterned non-close-packed photonic crystal film;
the surface-charged colloidal particles are one of surface-charged silicon dioxide, polystyrene or titanium dioxide spherical particles, and the particle size of the surface-charged colloidal particles is 50-150 nm;
the specific process of culturing the myocardial cells based on the non-close-packed photonic crystal film is to plant the myocardial cells on the surface of the non-close-packed photonic crystal film at 36-40 ℃ and 2-10 wt% of CO2Culturing for 2-10 days in a culture environment to obtain the non-close-packed photonic crystal film with the myocardial cells.
2. The method for detecting cardiomyocytes based on non-close-packed photonic crystal thin films according to claim 1, wherein: the biocompatible hydrogel precursor in the step 1) is one of polyacrylamide, poly-N-isopropylacrylamide, methacrylate gelatin, four-arm-polyethylene glycol or polyethylene glycol diacrylate.
3. The method for detecting cardiomyocytes based on non-close-packed photonic crystal thin films according to claim 1, wherein: the calculation formula of the myocardial beating frequency in the step 4) is that f is 1/t, wherein f is the myocardial beating frequency, and t is the single-cycle change time of the characteristic reflection peak; the magnitude of the contractile force of the myocardial cells is determined according to the magnitude of the characteristic reflection peak offset D lambda of the non-close-packed photonic crystal film, under the same culture condition, different stimuli are applied to the myocardial cells, and the larger the D lambda offset is, the larger the contractile force of the myocardial cells is.
4. The method for detecting cardiomyocytes based on non-close-packed photonic crystal thin films according to claim 1, wherein: the non-close-packed photonic crystal film in the step 1) has structural color, the structural color covers the visible light range, and the reflection peak position is between 400nm and 700 nm.
5. The method for detecting cardiomyocytes based on non-close-packed photonic crystal thin films according to claim 1, wherein the curing method in step ② is ultraviolet curing.
6. The method for detecting cardiomyocytes based on non-close-packed photonic crystal thin films according to claim 1, wherein: the myocardial cells are various myocardial cells obtained by different species and induced differentiation.
7. The use of the non-close-packed photonic crystal film-based cardiomyocyte detection method according to claim 1, wherein the method comprises: the method is applied to screening and evaluating the heart drugs.
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