CN107937259A - A kind of portable membrane type cell harvestor - Google Patents

A kind of portable membrane type cell harvestor Download PDF

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Publication number
CN107937259A
CN107937259A CN201711484661.6A CN201711484661A CN107937259A CN 107937259 A CN107937259 A CN 107937259A CN 201711484661 A CN201711484661 A CN 201711484661A CN 107937259 A CN107937259 A CN 107937259A
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CN
China
Prior art keywords
fluid cylinder
cylinder
membrane type
type cell
collection
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Pending
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CN201711484661.6A
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Chinese (zh)
Inventor
王亚东
刘址忠
郑浩轩
张悦华
王莉慧
白杨
武筱林
张熙
舒晓
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Southern Hospital Southern Medical University
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Southern Hospital Southern Medical University
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Priority to CN201711484661.6A priority Critical patent/CN107937259A/en
Publication of CN107937259A publication Critical patent/CN107937259A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention discloses a kind of portable membrane type cell harvestor, it is related to field of medical device, including cylinder cap, into fluid cylinder sum aggregate fluid cylinder, it is flexibly connected into fluid cylinder with collection fluid cylinder, cylinder cap is flexibly connected with into fluid cylinder, a stomata is provided with the side wall of cylinder cap and/or the side wall of collection fluid cylinder, soft rubber plug is provided with stomata, the lower end of fluid cylinder is provided with compressed part, compressed part is back taper mesa-shaped, the big end of compressed part is connected with the lower end into fluid cylinder, and it is provided with filter hole on the small end end face of compressed part, supporting network is fixed with by elastic webbing at collection fluid cylinder upper end opening, supporting network is corresponding with filtering hole site, nitrocellulose filter is placed with supporting network.The portable membrane type cell harvestor of the present invention, can be collected by filtration cell by shirtsleeve operation, and can accelerated filtration process, can also avoid carrying out cell in follow-up work the operation of secondary transferring, improve diagnosis efficiency, reduce rate of missed diagnosis.

Description

A kind of portable membrane type cell harvestor
Technical field
The present invention relates to field of medical device, more particularly to a kind of portable membrane type cell harvestor.
Background technology
The pathological diagnosis of human biopsy tissue specimen and cytology pathological diagnosis are the important components of diagnosing tumor.Especially It is alimentary canal histopathological diagnosis, relies primarily on living tissue materials pathologic finding under scope.But some cases are due to tumour Expansive growth in tissue lumen, often results in tube chamber obstruction, conventional Biopsy materials can not be used, at this time, by thin Born of the same parents' brush swipe tumour cell carries out cell pathology diagnosis and is just particularly important.
At present, the flaking method after cytobrush materials is using traditional Pap smear technology, as a kind of traditional Tabletting technology, in application process there are cell loss, slice-making quality is uneven, diagnosis difficulty is big the problems such as, particularly in peep Cell is collected under mirror draws materials, it is necessary to be realized by cytobrush, due to originals such as the length of endoscope is long, construction complexity, narrow spaces Cause, usually acquisition cell quantity is less, is diagnosed under sectioning cells and light microscopic and seems more difficult.Therefore, film-making matter how is improved It is particularly important that amount and positive diagnosing rate.
The content of the invention
To solve above technical problem, the present invention provides a kind of portable membrane type cell harvestor, can be by simply grasping Be collected by filtration cell, and can accelerated filtration process, can also avoid carrying out cell in follow-up work the operation of secondary transferring, Diagnosis efficiency is improved, reduces rate of missed diagnosis.
To achieve the above object, the present invention provides following scheme:
The present invention provides a kind of portable membrane type cell harvestor, including cylinder cap, into fluid cylinder sum aggregate fluid cylinder, it is described into fluid cylinder Upper end and it is described collection fluid cylinder upper end be opening, it is described into fluid cylinder be arranged at it is described collection fluid cylinder top, it is described into fluid cylinder Is flexibly connected with the collection fluid cylinder, the cylinder cap be arranged at it is described into the top of fluid cylinder, the cylinder cap with it is described movable into fluid cylinder A stomata is provided with the side wall of connection, the side wall of the cylinder cap and/or the collection fluid cylinder, soft rubber is provided with the stomata Rubber plug, the lower end into fluid cylinder are provided with compressed part, and the compressed part is back taper mesa-shaped, the big end of the compressed part with it is described Connected into the lower end of fluid cylinder, and filter hole is provided with the small end end face of the compressed part, led at the collection fluid cylinder upper end opening Cross elastic webbing and be fixed with supporting network, the supporting network is corresponding with the filtering hole site, and nitric acid is placed with the supporting network Cellulose filter membrane.
Further, the edge of the filter hole is equipped with downwardly projecting lower convex ring, is provided with the supporting network and institute The corresponding convex ring protruding upward of lower convex ring is stated, the lower convex ring and the convex ring are used to fix the nitrocellulose Filter membrane.
Further, the supporting network is arranged at the middle part of the collection fluid cylinder upper end opening.
Further, the size of the filter hole and the small end end face of the compressed part is in the same size.
Further, the side of the lower end into fluid cylinder is hinged by the upper end of articulation piece and the collection fluid cylinder, described Opposite side into the lower end of fluid cylinder is snapped connection by the upper end of fastener and the collection fluid cylinder, and the side of the cylinder cap passes through hinge Fitting is hinged with the upper end into fluid cylinder, and the opposite side of the cylinder cap is buckled by fastener with the upper end into fluid cylinder to be connected Connect.
Further, the upper end edge into fluid cylinder while and it is described collection fluid cylinder upper end edge while be equipped with sealing joint strip.
Further, the articulation piece is arranged at the same side, and the fastener is arranged at the same side.
Further, the fastener is elastic card fastener, and the articulation piece is hinge.
Further, the elastic webbing is provided with two and is symmetrical arranged, and the elastic webbing is sector, and the elastic webbing is Transparent elastic resin elastic webbing.
Further, the cylinder cap is transparent plastic cylinder, it is described into fluid cylinder be transparent plastics into fluid cylinder, the collection Fluid cylinder is transparent plastics collection fluid cylinder.
The present invention achieves following technique effect relative to the prior art:
1st, portable membrane type cell harvestor of the invention is set soft on the side wall of cylinder cap and/or the side wall of collection fluid cylinder Rubber stopper, can be inserted into fluid cylinder using syringe from the soft rubber plug on cylinder cap, to equipped with eluent into fluid cylinder Air is injected, is gradually stepped up into the air pressure in fluid cylinder, so as to accelerate the filtering of eluent, syringe can also be used from collection fluid cylinder In the soft rubber plug insertion collection fluid cylinder of side, gradually extraction collects the air in fluid cylinder to accelerate to filter, by above-mentioned simple Operation can be achieved to accelerate the purpose that process is collected by filtration, and convenient and efficient, efficiency is collected by filtration in raising.
2nd, portable membrane type cell harvestor of the invention is filtered using nitrocellulose filter, due to cellulose nitrate Homogeneous aperture response and the surface negative charge characteristic that plain filter membrane has, has epicyte protein suction-operated, containing cell to wash After the portable membrane type cell harvestor filtering that de- liquid passes through the present invention, cell will be enriched on nitrocellulose filter, nitric acid Cellulose membrane is as the final supporting body of cell, and nitrocellulose filter is also with stable physicochemical property, good hygrometric state Intensity, dye liquor absorption remain less and specially treated after can be transparent characteristic, current cytopathy can be directly carried out on the filter membrane The technical operations such as various dying operations and resin transparent, mounting needed for Neo-Confucianism diagnosis, compared with conventional cell collection method, are received Secondary transferring is not required in the cell of collection, avoids loss of the cell in collection, transfer process.Meanwhile after filtration directly Carry out the most cell pathologies and Cell Biology Experiment link such as dyeing, when reducing operation link and shortening operation Between, diagnosis efficiency is improved, reduces rate of missed diagnosis.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment Attached drawing to be used is needed to be briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention Example, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these attached drawings Obtain other attached drawings.
Fig. 1 is the structure diagram of portable membrane type cell harvestor of the present invention;
Fig. 2 is the structural decomposition diagram of portable membrane type cell harvestor of the present invention.
Description of reference numerals:1st, cylinder cap;2nd, into fluid cylinder;21st, compressed part;22nd, filter hole;3rd, fluid cylinder is collected;4th, soft rubber Plug;5th, articulation piece;6th, elastic webbing;7th, supporting network;8th, sealing joint strip;9th, fastener.
Embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work Embodiment, belongs to the scope of protection of the invention.
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, it is below in conjunction with the accompanying drawings and specific real Applying mode, the present invention is described in further detail.
As shown in Figs. 1-2, the present embodiment provides a kind of portable membrane type cell harvestor, including cylinder cap 1, into 2 and of fluid cylinder Collect fluid cylinder 3, the upper end into the upper end sum aggregate fluid cylinder 3 of fluid cylinder 2 is opening, and the top of collection fluid cylinder 3, feed liquor are arranged at into fluid cylinder 2 Cylinder 2 is flexibly connected with collection fluid cylinder 3, and cylinder cap 1 is arranged at into the top of fluid cylinder 2, cylinder cap 1 and is flexibly connected with into fluid cylinder 2, cylinder cap 1 A stomata is respectively arranged with the side wall of side wall sum aggregate fluid cylinder 3, stomata edge is equipped with outstanding arc-shaped edges, is set on stomata Have soft rubber plug 4, the lower end of fluid cylinder 2 be provided with compressed part 21, compressed part 21 is back taper mesa-shaped, the big end of compressed part 21 with Connected into the lower end of fluid cylinder 2, and filter hole 22 is provided with the small end end face of compressed part 21, the size and compressed part of filter hole 22 21 small end end face it is in the same size, collect and supporting network 7, supporting network 7 and mistake be fixed with by elastic webbing 6 at 3 upper end opening of fluid cylinder 22 position of filter opening is corresponding, and nitrocellulose filter is placed with supporting network 7, and supporting network 7 plays support to nitrocellulose filter Effect, avoids causing nitrocellulose filter to rupture because pressure is excessive.
In order to easily open and close by cylinder cap 1 and into fluid cylinder 2, into the lower end of fluid cylinder 2 side by articulation piece 5 with The upper end of collection fluid cylinder 3 is hinged, and the opposite side into the lower end of fluid cylinder 2 is snapped connection by fastener 9 with collecting the upper end of fluid cylinder 3, cylinder The side of lid 1 by articulation piece 5 with being hinged into the upper end of fluid cylinder 2, the opposite side of cylinder cap 1 by fastener 9 with into the upper of fluid cylinder 2 End snaps connection.In order to which sealing is better achieved, it is respectively provided with upper end edge of the upper end edge into fluid cylinder 2 in sum aggregate fluid cylinder 3 close Seal strip 8, when cylinder cap 1, covered together into 2 sum aggregate fluid cylinder 3 of fluid cylinder when so that device is internally formed confined space.
In this specific embodiment, articulation piece 5 is arranged at the same side, and fastener 9 is arranged at the same side, and articulation piece 5 is conjunction Page, fastener 9 is elastic card fastener.
In order to which nitrocellulose filter is fixed, the edge of filter hole 22 is equipped with downwardly projecting lower convex ring, support It is provided with net 7 and the corresponding convex ring protruding upward of lower convex ring, when being placed on the opening of collection fluid cylinder 3 into fluid cylinder 2, filtering 22 face supporting network 7 of hole, the compressed part 21 into the back taper mesa-shaped of fluid cylinder 2 are pressed downward supporting network 7, the down tensile elasticity of supporting network 7 Band 6, elastic webbing 6 is so that supporting network 7 presses below filter hole 22 all the time, and convex ring and lower convex ring are mutually pressed up and down at this time To close, the periphery of nitrocellulose filter is pressed between convex ring and lower convex ring, so that the periphery of nitrocellulose filter is clamped, Nitrocellulose filter is fixed, face covers filter hole 22 to nitrocellulose filter from below, so as to prevent filter process In unfiltered eluent flowed into from the side of filter hole 22 in collection fluid cylinder 3.
In this specific embodiment, supporting network 7 is arranged at the middle part of collection 3 upper end opening of fluid cylinder, and elastic webbing is symmetrically disposed on The both sides of supporting network 7, elastic webbing are sector, and the relatively smaller part arc-shaped edges of elastic webbing 6 are connected with half round edge of the side of supporting network 7, The larger semi-circle edge of elastic webbing 6 is connected with collecting the upper end edge side of fluid cylinder 3, fixes supporting network 7 so as to fulfill by elastic webbing 6 In the middle part of collection 3 upper end opening of fluid cylinder, elastic webbing 6 is made of transparent elastic resin.Cylinder cap 1, into 2 sum aggregate fluid cylinder 3 of fluid cylinder by Transparent plastics are made, by single unit system be arranged to it is transparent can be in order to observing experiment process.
Specifically used process is:Endoscope cell brush head is removed together together with plastic bushing first, is connected to disposable On syringe needle handle, promote the physiological saline in syringe to wash away brush head several times, then be placed in the oscillator vibrations several seconds, thus Obtain eluent, by obtained eluent inject the cell harvestor in the present embodiment into fluid cylinder 2, before injection, first will 3 upper end of collection fluid cylinder is placed on into fluid cylinder 2, and fastens and is in an open state into the fastener 9 between 2 sum aggregate fluid cylinder 3 of fluid cylinder, cylinder cap 1, After eluent all injection, cylinder cap 1 is covered, and fastens cylinder cap 1 and into the fastener 9 between fluid cylinder 2.According to actual needs, It is inserted into using syringe from the soft rubber plug 4 on cylinder cap 1 into fluid cylinder 2, to empty into injection in fluid cylinder 2 equipped with eluent Gas, gradually steps up into the air pressure in fluid cylinder 2, forms positive pressure, or the soft rubber plug 4 using syringe from 3 side of collection fluid cylinder In insertion collection fluid cylinder 3, gradually the air in extraction collection fluid cylinder 3, forms negative pressure, so as to accelerate the filtering of eluent, improves filtering Collection efficiency.After filtering, open into the fastener 9 between 2 sum aggregate fluid cylinder 3 of fluid cylinder, and the upper end for opening collection fluid cylinder 3 is opened Mouthful, the nitrocellulose filter collected and have cell is removed from supporting network 7, the liquid for collecting fluid cylinder 3, can fall from its side Go out.
Nitrocellulose filter in the present embodiment has filter membrane and adsorbed film function concurrently at the same time, especially has to albumen preferably Affinity characteristic, is collected, filter liquid and receipts by being collectively forming cell to the affine of memebrane protein, electrostatic interaction and H key active forces Collect cells Synchronous to complete.Further, since the suitable most cells of burn into of itself acid and alkali resistance of the filter membrane and part organic solvent Biology normal dyeing operate, and after can complete cellular morphology under an optical microscope, structure, functional expression and observation, can expire The clinical a variety of detections of foot, the needs of diagnosis, compared with conventional cell collection method, secondary transferring is not required in the cell of collection, keeps away Loss of the cell in collection, transfer process is exempted from.Meanwhile directly carry out most cell pathologies such as dyeing after filtration And Cell Biology Experiment link, reduce operation link and shorten the operating time, improve diagnosis efficiency, reduce Lou Examine rate.
Apply specific case in this specification to be set forth the principle of the present invention and embodiment, above example Explanation be only intended to help understand the present invention method and its core concept;Meanwhile for those of ordinary skill in the art, According to the thought of the present invention, in specific embodiments and applications there will be changes.In conclusion in this specification Appearance should not be construed as limiting the invention.

Claims (10)

1. a kind of portable membrane type cell harvestor, it is characterised in that described into fluid cylinder including cylinder cap, into fluid cylinder sum aggregate fluid cylinder Upper end and it is described collection fluid cylinder upper end be opening, it is described into fluid cylinder be arranged at it is described collection fluid cylinder top, it is described into fluid cylinder Is flexibly connected with the collection fluid cylinder, the cylinder cap be arranged at it is described into the top of fluid cylinder, the cylinder cap with it is described movable into fluid cylinder A stomata is provided with the side wall of connection, the side wall of the cylinder cap and/or the collection fluid cylinder, soft rubber is provided with the stomata Rubber plug, the lower end into fluid cylinder are provided with compressed part, and the compressed part is back taper mesa-shaped, the big end of the compressed part with it is described Connected into the lower end of fluid cylinder, and filter hole is provided with the small end end face of the compressed part, led at the collection fluid cylinder upper end opening Cross elastic webbing and be fixed with supporting network, the supporting network is corresponding with the filtering hole site, and nitric acid is placed with the supporting network Cellulose filter membrane.
2. portable membrane type cell harvestor according to claim 1, it is characterised in that the edge of the filter hole is equipped with Downwardly projecting lower convex ring, be provided with the supporting network with the corresponding convex ring protruding upward of the lower convex ring, it is described Lower convex ring and the convex ring are used to fix the nitrocellulose filter.
3. portable membrane type cell harvestor according to claim 1, it is characterised in that the supporting network is arranged at described Collect the middle part of fluid cylinder upper end opening.
4. portable membrane type cell harvestor according to claim 1, it is characterised in that the size of the filter hole and institute State the in the same size of the small end end face of compressed part.
5. portable membrane type cell harvestor according to claim 1, it is characterised in that the one of the lower end into fluid cylinder Side is hinged by the upper end of articulation piece and the collection fluid cylinder, and the opposite side of the lower end into fluid cylinder passes through fastener and the collection The upper end of fluid cylinder snaps connection, and the side of the cylinder cap is hinged by articulation piece and the upper end into fluid cylinder, the cylinder cap Opposite side is snapped connection by fastener and the upper end into fluid cylinder.
6. portable membrane type cell harvestor according to claim 5, it is characterised in that the upper end edge side into fluid cylinder Sealing joint strip is equipped with the upper end edge side of the collection fluid cylinder.
7. portable membrane type cell harvestor according to claim 5, it is characterised in that the articulation piece is arranged at same Side, the fastener are arranged at the same side.
8. portable membrane type cell harvestor according to claim 7, it is characterised in that the fastener is Elastic buckle Part, the articulation piece are hinge.
9. the portable membrane type cell harvestor according to any one of claim 1-8, it is characterised in that the elastic webbing It is provided with two and is symmetrical arranged, the elastic webbing is sector, and the elastic webbing is transparent elastic resin elastic webbing.
10. the portable membrane type cell harvestor according to any one of claim 1-8, it is characterised in that the cylinder cap For transparent plastic cylinder, described into fluid cylinder is transparent plastics into fluid cylinder, and the collection fluid cylinder is transparent plastics collection fluid cylinder.
CN201711484661.6A 2017-12-29 2017-12-29 A kind of portable membrane type cell harvestor Pending CN107937259A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113116397A (en) * 2019-12-30 2021-07-16 上海科罡医疗技术有限公司 Esophageal wall cell sampler

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Publication number Priority date Publication date Assignee Title
CN113116397A (en) * 2019-12-30 2021-07-16 上海科罡医疗技术有限公司 Esophageal wall cell sampler
CN113116397B (en) * 2019-12-30 2023-02-28 上海科罡医疗技术有限公司 Esophageal wall cell sampler

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