CN107936098B - Chlorella ellipsoidea NF-YA gene and application thereof - Google Patents
Chlorella ellipsoidea NF-YA gene and application thereof Download PDFInfo
- Publication number
- CN107936098B CN107936098B CN201711396992.4A CN201711396992A CN107936098B CN 107936098 B CN107936098 B CN 107936098B CN 201711396992 A CN201711396992 A CN 201711396992A CN 107936098 B CN107936098 B CN 107936098B
- Authority
- CN
- China
- Prior art keywords
- gene
- chlorella ellipsoidea
- protein
- plant
- cenf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides a chlorella ellipsoidea NF-YA gene and application thereof, belonging to the technical field of microalgae genetic engineering. The NF-YA gene sequence of the Chlorella ellipsoidea is shown in SEQ ID No.1, and the coding protein sequence thereof is shown in SEQ ID No. 2. The gene is derived from Chlorella ellipsoidea (Chlorella ellipsoidea), and is transformed into Arabidopsis thaliana, and the result shows that the total fatty acid content of the Arabidopsis thaliana transformed with CeNF-YA is improved by 18.4-22.41 percent compared with that of wild Col-0, and the thousand seed weight is increased by 26.5-44.9 percent compared with that of wild Col-0. The gene of the chlorella ellipsoidea NF-YA can greatly improve the total oil content of plant cells, can be applied to the field of biological oil preparation and crop yield improvement, and has good application prospect.
Description
Technical Field
The invention belongs to the field of microalgae genetic engineering, and particularly relates to a chlorella ellipsoidea NF-YA gene, and a coding protein and application thereof.
Background
With the excessive consumption of traditional energy and the increasing energy demand, the energy problem is becoming more serious, and the research of alternative energy becomes a focus of attention. Microalgae are easy to culture due to high oil content, and the oil yield of unit land is far higher than that of other oil crops, so that microalgae are considered as one of energy resources with optimal potential.
The microalgae is a unicellular lower plant, has strong reproductive capacity, low requirement on growth environment, easy culture, high photosynthesis efficiency and high oil content. Triacylglycerols (TAGs) in microalgal cells can reach 20% -50% of dry weight. Triacylglycerols (TAGs) are the main storage forms of plant storage lipids, are widely distributed in organs such as seeds, pollen, fruits, leaves and the like of plants, and mainly participate in numerous vital activities such as cell membrane lipid construction, energy metabolism, stress reaction, seed germination, pollen development and the like. Therefore, microalgae have excellent candidate gene resources related to oil and fat metabolism. The NF-Y (nuclear Factor Y) super gene family is a transcription Factor family which can combine CCAAT-box and is widely existed in eukaryote, and is composed of 3 gene families, namely NF-YA, NF-YB and NF-YC. In higher plants, the members of the gene family have important influence on the synthesis and accumulation of oil and fat, the growth and development of plants, stress resistance and the like. It has been shown that higher Plant-derived NF-Y genes driven by the CaMV35S promoter or other seed-specific promoters such as the unmodified Napin promoter cause dysplasia or abnormal seed germination (Mu et al, 2008, Plant Physiology,148: 1042-1584; Tan et al, 2011, Plant Physiology, 156: 1577-1588). This limits the application of the gene. At present, no relevant research report exists about NF-YA genes and functions thereof in Chlorella ellipsoidea.
Disclosure of Invention
The invention aims to provide Chlorella ellipsoidea (Chlorella ellipsoidea) NF-YA and application of a coding protein thereof.
The present invention first provides chlorella ellipsoidea NF-YA protein having:
1) an amino acid sequence shown as SEQ ID No. 2; or
2) Protein which is derived from the protein 1) and has the same activity and is obtained by substituting, deleting and/or adding one or more amino acids in the amino acid sequence shown in SEQ ID No. 2.
The present invention provides a gene encoding chlorella ellipsoidea NF-YA protein, having:
1) a nucleotide sequence shown as SEQ ID No. 1; or
2) The nucleotide sequence shown in SEQ ID No.1 is substituted, deleted and/or added with one or more nucleotides; or
3) Nucleotide sequences which hybridize under stringent conditions with the DNA sequences defined in 1).
The invention provides a biological material containing the gene for coding the chlorella ellipsoidea NF-YA protein, and the biological material is a vector, a host cell or an expression cassette.
The invention provides application of the chlorella ellipsoidea NF-YA protein or a gene encoding the protein or a biological material containing the gene in improving the total fatty acid content in cells.
The cell is a plant cell.
The invention provides application of the chlorella ellipsoidea NF-YA protein or a gene encoding the protein or a biological material containing the gene in preparation of transgenic plants with high total fatty acid content.
The plant is oil crop. The oil crops are rape, arabidopsis, sunflower, soybean, tomato, castor, palm, sesame or peanut.
The invention provides application of the chlorella ellipsoidea NF-YA protein or a gene encoding the protein or a biological material containing the gene in improving thousand seed weight of plant seeds. The plant is rape, arabidopsis, sunflower, soybean, tomato, palm, castor-oil plant, sesame, peanut, rice, wheat or corn.
The invention provides application of the chlorella ellipsoidea NF-YA protein or a gene encoding the protein or a biological material containing the gene in improvement of plant germplasm resources.
The invention provides application of the chlorella ellipsoidea NF-YA protein or a gene encoding the protein or a biological material containing the gene in producing edible oil.
The invention provides the application of the chlorella ellipsoidea NF-YA protein or the gene encoding the protein or the biological material containing the gene in producing biodiesel.
According to the invention, a cDNA sequence of CeNF-YA is screened according to the annotation of a transcriptome of Chlorella ellipsoidea, mRNA of the Chlorella ellipsoidea is extracted, after reverse transcription, a full-length CDS sequence of the CeNF-YA is cloned and is constructed on an entry vector, then the CDS sequence is constructed on a plant expression vector through recombination reaction, positive clone is screened, and the plant is infected after agrobacterium is transformed (GV 3101). The result shows that the CeNF-YA which has the nucleotide sequence of SEQ ID NO.1 and is derived from the Chlorella ellipsoidea is heterogeneously expressed in the Arabidopsis thaliana under the drive of the CaMV35S promoter, the transgenic Arabidopsis thaliana has no undesirable agronomic characters such as inhibiting the growth and development of the Arabidopsis thaliana, and the oil content and the thousand seed weight of the transgenic Arabidopsis thaliana seed are obviously improved: the total fatty acid content of the Arabidopsis transformed with CeNF-YA is improved by 18.4-22.41 percent compared with that of the wild Col-0; the thousand kernel weight is increased by 26.5 to 44.9 percent compared with the wild type. The arabidopsis thaliana is a model plant, and genes which can play a role in arabidopsis thaliana have similar effects in various crops, so that the chlorella ellipsoidea CeNF-YA gene with the nucleotide sequence of SEQ ID NO.1 can be used for genetic breeding of oilseed rape, soybean, cotton, peanut, palm and other oil plants to improve the oil content and the thousand seed weight, and for breeding of wheat, rice, corn and other crops to improve the thousand seed weight.
Drawings
FIG. 1 is a map of entry vector of CeNF-YA gene.
FIG. 2 is a CeNF-YA gene plant expression vector map.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 obtaining of Chlorella ellipsoidea NF-YA Gene and construction of expression vector
1. Extraction of total RNA of Chlorella ellipsoidea
Collecting sufficient Chlorella ellipsoidea cells, rapidly adding liquid nitrogen, grinding with mortar, collecting 50-100mg powder, adding 1m L Trizol (Invitrogen) extraction buffer solution, mixing, standing for 10min, adding 0.2m L chloroform, mixing, centrifuging at 13,500rpm for 10min, collecting supernatant, adding 0.2m L chloroform, mixing, centrifuging at 13,500rpm for 10min, collecting supernatant, adding half volume of isopropanol, standing at room temperature for 30-50min, centrifuging at 13,500rpm for 10min, discarding supernatant, adding 75% ethanol, suspending and precipitating at 10,000rpm for 5min, discarding supernatant, adding 100% ethanol, suspending and precipitating, centrifuging at 10000rpm for 5min, discarding supernatant, blow-drying in ultra-clean bench (more than 3-5min), adding 50 μ L DEPC water to dissolve at-80 deg.C, and storing at-80 deg.C
2. Synthesis of Chlorella ellipsoidea cDNA
cDNA is produced by mRNA by using a DNA-removing reverse transcription kit of the whole gold company, wherein the system comprises 5 mu g of totalRNA, 50 mMOoligo (dT18), 10 mu L2 × TS Reaction Mix, 1 mu L TransScript RT/RI Enzyme Mix and 1 mu L g of DNA Redmover, the materials are supplemented to 20 mu L by RNase-free water, the materials are gently mixed, incubated at 42 ℃ for 40-50min and inactivated at 85 ℃ for 5 min-20 ℃ and stored for standby.
3. Obtaining of CeNF-YA gene and construction of expression vector
The complete CDS is predicted according to the cDNA sequence in the transcriptome, translated into an amino acid sequence, and then compared in an NCBI protein database to further determine the integrity of the CeNF-YA gene. cDNA is used as a template, upstream and downstream amplification primers (an upstream primer 5-AGCAGGCTTTGACTTTATGGCTCCCATCGAGAAGAAG-3 and a downstream primer 5-TGGGTCTAGAGACTTTCCGGATTTTGATGCATCCTGCTGTT-3) are designed, and the CeNF-YA gene is amplified by using high-fidelity Taq enzyme. The amplification procedure was 2min at 98 ℃ for pre-denaturation, 30s at 98 ℃, 30s at 60 ℃, 30s at 72 ℃ for 35 cycles. And purifying the PCR product for later use.
The PCR product is connected to an entry vector (named pGWC-CeNF-YA, figure 1) by utilizing an In-fusion system, specifically comprises pGWCm (100 ng/mu L cut by AhdI) after 1 mu L enzyme cutting, a 1 mu L PCR product (80 ng/mu L), 2 mu L In-fusion Mix at 50 ℃ for 50-60min, escherichia coli DH5 α, positive clones are identified and screened by PCR, and are constructed on a plant expression vector through a gateway system after sequencing identification, and the recombinant plasmid is transformed into agrobacterium GV3101 and is used after PCR identification.
Example 2 genetic transformation of CeNF-YA Gene and screening of Positive transgenic lines
Agrobacterium with pCeNF-YA plasmid prepared in example 1 was cultured in L B broth (containing 50 mg/L kanamycin, 50 mg/L gentamicin and 200 mg/L rifampicin) to OD 0.8, centrifuged at 10000rpm for 5min to collect the cells, and an equal volume of MgCl suspension (10mM MgCl) was used25% sucrose), centrifugally collecting thalli, suspending the thalli by using a suspension until OD is 1.0, adding 0.005% Silwet L-77, transforming arabidopsis thaliana at the early stage of flowering once, dipping flowers once again at intervals of 7 days, collecting seeds of T0 generations after the seeds are mature, planting the seeds in a culture dish, spraying Barsta (0.3%) for screening 10 days after emergence of seedlings, spraying again at intervals of 5 days, identifying by PCR, transplanting positive seedlings of T1 generations into a nutrition pot for culture, and collecting the seeds after the positive seedlings are mature.
Example 3 analysis of fatty acid composition in seeds transformed with CeNF-YA Gene
The transgenic Arabidopsis seeds of example 2 were collected, dried at 37 ℃ and ground thoroughly, 0.05g was weighed, and 3m L7.5.5% KOH-CH was added3OH (C17: 0 standard substance is added as internal reference), water bath is carried out at 70 deg.C for 3-5h, the middle part is reversed and mixed for several times, 2m L HCl-CH is added3OH (V/V, 1:1) solution, 2m L14% BF3-CH3And (3) adding 1m L0.9% NaCl and 4m L n-hexane into the OH solution in a water bath at 70 ℃ for 1.5h, fully oscillating and uniformly mixing, centrifuging at 4,000rpm for 8min, transferring an upper organic phase into a new tube, drying by nitrogen, dissolving by 300 mu L ethyl acetate, and repeating the experiment for 3 times in parallel by making two samples each time.
The GC/MS instrument equipment is started strictly according to the operating specification of TurboMass (PerkinElmer company), the GC parameters are set as follows, a chromatographic column is BPX-70, 30m × 0.25.25 mm × 0.25.25 mu m, the temperature of a column box is set as gradient temperature rise (100 ℃, 1min, 15 ℃/min to 190 ℃, 1min, 10 ℃/min to 220 ℃, 4min), carrier gas is helium, the flow is 1m L/min, a 1 mu L sample is taken for GC-MS detection, the peak areas corresponding to different fatty acids are compared with the internal standard of the C17:0 peak area according to the gas chromatographic analysis result to calculate the content of each fatty acid component and the total fatty acid content, and the fatty acid analysis of different transgenic lines is shown in Table 1.
TABLE 1 fatty acid composition of transgenic lines (μ g/mg)
From experimental results, the fatty acid composition of the CeNF-YA transgenic arabidopsis seeds is not changed, but the content of the CeNF-YA transgenic arabidopsis seeds is obviously changed, the total fatty acid content of the CeNF-YA transgenic arabidopsis seeds is improved by 18.4-22.41% compared with that of wild Col-0, the C18:0 is improved by 14.5-24.4%, the C18:1 is improved by 10.5-31.0%, the C18:3 is improved by 27.2-35.2%, and the C20:1 is improved by 27.4-34.8%.
Example 4 thousand kernel weight analysis of transgenic CeNF-YA Gene
The transgenic Arabidopsis seeds of example 2 were collected, dried at 37 ℃ and then counted for 1,000 seeds, and each line was repeated 3 times. The thousand grain weights of the Col-0, CeNF-YA-38, CeNF-YA-46 and CeNF-YA-47 are respectively 16.33 +/-2.08 mg, 23.67 +/-2.31 mg, 23 +/-3.46 mg and 22.33 +/-0.58 mg. From experimental results, the thousand grain weight of arabidopsis can be obviously increased by transferring the CeNF-YA gene, and the thousand grain weight is increased by 26.5% -44.9% compared with that of a wild type.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Sequence listing
<110> institute of genetics and developmental biology of Chinese academy of sciences
<120> Chlorella ellipsoidea NF-YA gene and application thereof
<130>KHP171118897.3
<160>4
<170>SIPOSequenceListing 1.0
<210>1
<211>349
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>1
atggctccca tcgagaagaa ggccagcaaa aaggaggagt ctgcatttgg gctgatcttg 60
cggctgcaca aggtgaagaa gctcatcaag tcggggaata gcgtcaaggc tgtctcaggg 120
gaggccagct ttgccattgc gagatccacg gagctcttgg tggagatgct ggtcgcaaag 180
gcttaccata aaatggttgc agactccaga gacatgattg ggtacgacga tgtagcctct 240
gccgtcacca attggccggc caccgacttt ttgagagata ttgtgccgga gacactgaca 300
ttgaatgagc tcattcaatt ggccaaacag caggatgcat caaaattag 349
<210>2
<211>117
<212>PRT
<213> Artificial Sequence (Artificial Sequence)
<400>2
Met Ala Pro Ile Glu Lys Lys Ala Ser Lys Lys Glu Glu Ser Ala Phe
1 5 10 15
Gly Leu Ile Leu Arg Leu His Lys Val Lys Lys Leu Ile Lys Ser Gly
20 25 30
Asn Ser Val Lys Ala Val Ser Gly Glu Ala Ser Phe Ala Ile Ala Arg
35 40 45
Ser Thr Glu Leu Leu Val Glu Met Leu Val Ala Lys Ala Tyr His Lys
50 55 60
Met Val Ala Asp Ser Arg Asp Met Ile Gly Tyr Asp Asp Val Ala Ser
65 70 75 80
Ala Val Thr Asn Trp Pro Ala Thr Asp Phe Leu Arg Asp Ile Val Pro
85 90 95
Glu Thr Leu Thr Leu Asn Glu Leu Ile Gln Leu Ala Lys Gln Gln Asp
100 105 110
Ala Ser Lys Leu Xaa
115
<210>3
<211>37
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>3
agcaggcttt gactttatgg ctcccatcga gaagaag 37
<210>4
<211>41
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>4
tgggtctaga gactttccgg attttgatgc atcctgctgt t 41
Claims (9)
1. The chlorella ellipsoidea NF-YA protein is characterized in that the amino acid sequence is shown as SEQ ID No. 2.
2. The gene for coding the protein of claim 1, and the nucleotide sequence of the gene is shown as SEQ ID No. 1.
3. Biological material comprising the gene of claim 2, said biological material being a vector, a host cell or an expression cassette, said host cell being incapable of being propagated as a plant or an animal.
4. Use of the chlorella ellipsoidea NF-YA protein of claim 1 or the gene of claim 2 or the biomaterial of claim 3 for increasing the total fatty acid content in a plant cell.
5. Use of the chlorella ellipsoidea NF-YA protein of claim 1 or the gene of claim 2 or the biomaterial of claim 3 for the preparation of transgenic plants with high total fatty acid content.
6. Use of the chlorella ellipsoidea NF-YA protein of claim 1 or the gene of claim 2 or the biological material of claim 3 for increasing the thousand kernel weight of plant seeds.
7. Use of the chlorella ellipsoidea NF-YA protein of claim 1 or the gene of claim 2 or the biomaterial of claim 3 for improving plant germplasm resources.
8. Use of the chlorella ellipsoidea NF-YA protein of claim 1 or the gene of claim 2 or the biomaterial of claim 3 for the production of edible oil.
9. Use of the chlorella ellipsoidea NF-YA protein of claim 1 or the gene of claim 2 or the biomaterial of claim 3 for the production of biodiesel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711396992.4A CN107936098B (en) | 2017-12-21 | 2017-12-21 | Chlorella ellipsoidea NF-YA gene and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711396992.4A CN107936098B (en) | 2017-12-21 | 2017-12-21 | Chlorella ellipsoidea NF-YA gene and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107936098A CN107936098A (en) | 2018-04-20 |
| CN107936098B true CN107936098B (en) | 2020-07-14 |
Family
ID=61942113
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711396992.4A Expired - Fee Related CN107936098B (en) | 2017-12-21 | 2017-12-21 | Chlorella ellipsoidea NF-YA gene and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107936098B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7294759B2 (en) * | 2001-06-29 | 2007-11-13 | E. I. Du Pont De Nemours And Company | Alteration of oil traits in plants |
| CN102277378B (en) * | 2011-08-17 | 2013-07-24 | 中国科学院遗传与发育生物学研究所 | Method for increasing total oil and fat and linoleic acid (LA) or alpha-linoleic acid (ALA) content in chlorella |
| CN102532291B (en) * | 2012-01-10 | 2014-05-14 | 中国科学院遗传与发育生物学研究所 | NF-YAI protein and application of coding gene thereof in cultivating plant with improved content of fatty acid |
| CN104988158B (en) * | 2015-07-15 | 2018-09-18 | 南京农业大学 | Cabbage type rape nuclear factor NF-YA genes BnNF-YA12 and its application |
-
2017
- 2017-12-21 CN CN201711396992.4A patent/CN107936098B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN107936098A (en) | 2018-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108101973B (en) | Chlorella ellipsoidea NF-YC gene and application thereof | |
| CN105859860A (en) | Application of disease resistance-related protein to regulation and control of plant disease resistance | |
| CN110117320A (en) | Cotton GhCAL-D07 gene is promoting the application in flowering of plant | |
| US9816099B2 (en) | Gene for increasing plant weight and method for using the same | |
| CN103468714B (en) | Application of rice PS1 protein and rice PS1 protein coding gene in adjustment of plant senescence | |
| CN105985416A (en) | Wax development regulative gene CFLAP1 and application thereof in drought resistance of plants | |
| JPWO2009072608A1 (en) | Genes for increasing production of plant oils and methods of use thereof | |
| CN102154337B (en) | Gossypium hirsutum mitogen-activated protein kinas 6 (GhMAPK6) gene and application thereof | |
| CN107936098B (en) | Chlorella ellipsoidea NF-YA gene and application thereof | |
| CN101597329B (en) | Transcription factor relevant with vegetable fatty acid and oil metabolism as well as encoding gene and application thereof | |
| CN108003226B (en) | Chlorella ellipsoidea NF-YB gene and application thereof | |
| CN110117321A (en) | Cotton GhDctpp1-D11 gene is promoting the application in flowering of plant | |
| CN102070706B (en) | Protein AtL1L related to seed fatty acid synthesis as well as encoding gene and applications thereof | |
| WO2021047377A1 (en) | Application of tpst gene in regulation of traits of plant | |
| CN107312077A (en) | Albumen and the application of wax plum CpSOC1 genes and its coding | |
| CN104877021A (en) | Rapeseed transcription factor BnFUS3 related to plant fatty acid and fat metabolism and coding gene and application thereof | |
| CN113604485B (en) | Arabidopsis AtGSNOR gene, protein and application | |
| CN103320468B (en) | UCH320 protein and application of coding gene thereof in adjusting and controlling plant growth and development | |
| CN117402888A (en) | Salt-tolerant gene MsMYB112 of miscanthus sinensis and application thereof | |
| CN114480435A (en) | Application of epiphyte of photinia stonifolia to improvement of abiotic stress resistance of plants | |
| CN117737116A (en) | Alfalfa MsSAP26 gene and application | |
| CN117683809A (en) | Yield-related protein OsSOE, biological material and application thereof | |
| CN101851282A (en) | Protein MtCBF4 associated with stress tolerance of plants, encoding gene and application thereof | |
| Fan | Genetic engineering of ORSV-resistant Phalaenopsis | |
| Zhang et al. | An Efficient Method to Screen for Salt Tolerance Genes in Salt Cress |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200714 Termination date: 20211221 |