CN107936085A - Using ultraviolet irradiation in TiO2The method and TiO of upper ankyrin and regulating cell compatibility2Protein product - Google Patents
Using ultraviolet irradiation in TiO2The method and TiO of upper ankyrin and regulating cell compatibility2Protein product Download PDFInfo
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- CN107936085A CN107936085A CN201711167358.3A CN201711167358A CN107936085A CN 107936085 A CN107936085 A CN 107936085A CN 201711167358 A CN201711167358 A CN 201711167358A CN 107936085 A CN107936085 A CN 107936085A
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- tio
- ultraviolet irradiation
- protein
- ankyrin
- regulating cell
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/042—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers characterised by the nature of the carrier
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/75—Fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
Abstract
One kind is using ultraviolet irradiation in TiO2The method and TiO of upper ankyrin and regulating cell compatibility2Protein product, is related to technical field of biological material.The embodiment of the present invention utilizes ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility is by TiO2Material is placed in protein solution, makes TiO2Adsorption albumen, takes out and washes with water, and washes unadsorbed albumen off, obtains TiO2Protein sample；By TiO2Protein sample carries out the ultraviolet irradiation of dry or wet, obtains the TiO with different cellular affinities2Protein product, this is using ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility can be with Effective Regulation TiO2The cellular affinity of adsorption albumen；Obtained TiO2Protein product can meet individual demand of the different biomaterial application fields to material biology performance, have important practical value.
Description
Technical field
The present invention relates to technical field of biological material, and more particularly to one kind utilizes ultraviolet irradiation in TiO2Upper ankyrin is simultaneously adjusted
Control the method and TiO of cellular affinity2- protein product.
Background technology
Protein is the basic component for forming organism, each process in cell activities has participation
Effect.The biocompatibility of material uniqueness and diversified biological function can be provided in material surface ankyrin, from
And applied in multiple fields such as such as bioelectronics, organizational project, insoluble drug releases.
Mainly there are two classes in the method for material surface ankyrin at present：(1) self-assembly method：There is self assembly using minority
The albumen of characteristic, such as fibroin albumen, carry out self assembly in material surface and fix；(2) Crosslink bond type：Using crosslinking agent by albumen
It is fixed on material surface.Since the protein classes with self assembly characteristic are less, its application is subject to serious first method
Limitation；Second method then there is the complete crosslinking agent of unreacted to remain in protein films, damage protein films biofacies
The shortcomings that capacitive.The method handled using gas ions is the means of novel proteopexy a kind of in recent years, specifically in material
Surface generates free radicals, and utilizes free radical ankyrin, and abbreviation free radical fixation can be in material surface using this method
Firm covalent ankyrin, and maintain the native configurations of albumen.Relative to self-assembly method and Crosslink bond type, free radical fixation
Suitable for most of albumen, and cross-linking agent-free remains, and has significant advantage.
After ankyrin, the cellular affinity of further controlled material surface protein has highly important meaning
Justice.This is because different biomaterials has completely different requirement for its cellular affinity：Such as Integrated implant material, it is desirable to
High osteocyte affinity, to promote Integrated implant reparation；And contacting blood material, then low blood cell affinity is required, with
Prevent thrombosis.Recent domestic report related to this mainly has：Alwin M.D.Wan et al. are once using voltage and electricity
The mode of stream controls the change of configuration of fibronectin, Eunhee Jeoung et al. then to be received in control temperature and air pressure use at the same time
The configuration and cellular affinity of rice stamping technique regulation and control multiple protein.Generally speaking, up to the present, still rare report both at home and abroad
By simply changing treatment conditions, the technological means of lifting/reduction albuminous cell compatibility bidirectional modulation is realized.
Therefore go to regulate and control to manipulate the cellular affinity of material surface adhesion protein there is an urgent need to a kind of simple technological means.
The content of the invention
It is an object of the invention to provide one kind using ultraviolet irradiation in TiO2Upper ankyrin and regulating cell compatibility
Method, effectively regulate and control TiO2The cellular affinity of adsorption albumen or the means irradiated by mask, obtain cell
Micrographics.
Another object of the present invention is to provide a kind of TiO2- protein product, can meet different biomaterial application fields
To the individual demand of material biology performance, there is important practical value.
The present invention is solved its technical problem and is realized using following technical scheme.
The present invention proposes one kind using ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, it is wrapped
Include following steps：
By TiO2Material is placed in protein solution, makes TiO2Adsorption albumen, takes out and washes with water, and washes off unadsorbed
Albumen, obtain TiO2- protein sample；
By TiO2- protein sample carries out the ultraviolet irradiation of dry or wet, obtains the TiO with different cellular affinities2-
Protein product.
Further, in present pre-ferred embodiments, TiO2Material is TiO2Film, TiO2Particle or TiO2Nanotube.
Further, in present pre-ferred embodiments, albumen includes albumin, fibrinogen, collagen, peroxide
Change hydrogen enzyme or superoxide anion mutase.
Further, in present pre-ferred embodiments, water used is pure water or the salting liquid of water.
Further, in present pre-ferred embodiments, ultraviolet irradiation is full ultraviolet irradiation or mask ultraviolet irradiation.
Further, in present pre-ferred embodiments, the specific method of the ultraviolet irradiation of dry method is：First by TiO2- egg
White sample drying, then ultraviolet irradiation is carried out in dry environment.
Further, in present pre-ferred embodiments, dry method is to spontaneously dry or be freeze-dried in atmosphere.
Further, in present pre-ferred embodiments, the specific method of the ultraviolet irradiation of wet method is：By moistening
TiO2- protein sample carries out ultraviolet irradiation in water.
A kind of TiO2- protein product, it is to utilize ultraviolet irradiation in TiO according to above-mentioned2Upper ankyrin and regulating cell
The method of compatibility is made.
Further, in present pre-ferred embodiments, ultraviolet irradiation is full ultraviolet irradiation, corresponding TiO2- albumen produces
Product is promotions or suppress follow-up albumen, the protein surface that cell adsorbs；Ultraviolet irradiation is mask ultraviolet irradiation, corresponding TiO2-
Protein product is promotion or the micro-patterning protein surface of suppression cell adhesion protein-general proteins.
The embodiment of the present invention utilizes ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility and
TiO2The beneficial effect of-protein product is：The embodiment of the present invention utilizes ultraviolet irradiation in TiO2Upper ankyrin and regulating cell
The method of compatibility is by TiO2Material is placed in protein solution, makes TiO2Adsorption albumen, takes out and washes with water, wash off
Unadsorbed albumen, obtains TiO2- protein sample；By TiO2- protein sample carries out the ultraviolet irradiation of dry or wet, is had
There is the TiO of different cellular affinities2- protein product, this is using ultraviolet irradiation in TiO2Upper ankyrin and regulating cell compatibility
Method can be with Effective Regulation TiO2The cellular affinity of adsorption albumen or the means irradiated by mask, obtain cell
Micrographics；Obtained TiO2- protein product can meet personalization of the different biomaterial application fields to material biology performance
Demand, has important practical value.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not construed as pair
The restriction of scope, for those of ordinary skill in the art, without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 regulates and controls TiO for embodiment 1-4 using wet method/dry method ultraviolet irradiation2Surface protein cellular affinity schematic diagram；
Fig. 2 represents embodiment 1 using the full ultraviolet irradiation regulation and control TiO of wet method2Surface protein cellular affinity result；
Fig. 3 represents embodiment 2 using the full ultraviolet irradiation regulation and control TiO of dry method2Surface protein cellular affinity result；
Fig. 4 regulates and controls TiO for embodiment 5 using wet method/dry method mask ultraviolet irradiation2Surface protein cellular affinity is illustrated
Figure；
Fig. 5 represents embodiment 5 using dry/wet mask ultraviolet irradiation regulation and control TiO2Surface protein cellular affinity knot
Fruit.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer, is the conventional production that can be obtained by commercially available purchase
Product.
Ultraviolet irradiation is utilized in TiO to the embodiment of the present invention below2The side of upper ankyrin and regulating cell compatibility
Method and TiO2- protein product is specifically described.
The embodiment of the present invention provides one kind using ultraviolet irradiation in TiO2The side of upper ankyrin and regulating cell compatibility
Method, it comprises the following steps：
S1, by TiO2Material is placed in protein solution, TiO2Material can be TiO2Film, TiO2Particle or TiO2Nanometer
Pipe etc., albumen can be arbitrary protein, specifically include the multiple proteins such as albumin, fibrinogen or collagen, or mistake
A variety of enzymes such as hydrogen oxide enzyme, superoxide anion mutase, make TiO2Adsorption albumen, takes out the TiO after adhesion protein2It is used in combination
Water cleans, and water used can be pure water, such as RO water, UP water, or the salting liquid of water, such as phosphate buffered physiological brine
Deng washing unadsorbed albumen off, obtain TiO2- protein sample.
S2, by TiO2- protein sample carries out the ultraviolet irradiation of dry or wet, and ultraviolet irradiation is full ultraviolet irradiation or mask
Ultraviolet irradiation, obtains the TiO with different cellular affinities2- protein product.Specifically, full ultraviolet irradiation is corresponded to and is promoted
Or suppress follow-up albumen, the protein surface of cell absorption, i.e. TiO2- albumen composite surface；Mask ultraviolet irradiation is to utilize mask
Plate is into line mask ultraviolet irradiation, so as to obtain the protein pattern with different cellular affinities, and is further made and promotes or press down
The micro-patterning protein surface of cell adhesion protein-general proteins processed, i.e. TiO2- albuminous cell micrographics surface.
If by TiO2- protein sample carries out the ultraviolet irradiation of dry method, and step S2 specifically includes procedure below：
S2A, by TiO2- protein sample is dried, and dry specific method is to spontaneously dry or be freeze-dried in atmosphere.
S2B, by dried TiO2- protein sample carries out the full ultraviolet irradiation of dry method in dry environment or dry method mask is purple
External irradiation, obtains the TiO with good cellular affinity2- albumen composite surface or TiO2- albuminous cell micrographics surface.
If by TiO2- protein sample carries out the ultraviolet irradiation of wet method, and step S2 specifically includes procedure below：
S2a, the TiO by moistening2- protein sample carries out the full ultraviolet irradiation of wet method or wet method mask ultraviolet irradiation in water,
Obtain the TiO with resistance cell adherence ability (i.e. cellular affinity is relatively low)2- albumen composite surface or TiO2- albuminous cell
Micrographics surface.
The present embodiment is usually first by the TiO of moistening2- protein sample is dried, in order to Sample storage, further according to demand into
The ultraviolet irradiation of row dry method, or by dried TiO2- protein sample soaks again, carries out the ultraviolet irradiation of wet method.
Since the cellular affinity of albumen has close relationship with its configuration, and water is the driving force of protein folding.Egg
For the amino residue that the hydrate water of adsorption can be directly with protein surface in vain by hydrogen bond action, this is to influence protein configurations
Key factor.Research shows that dry albumen and the albumen of wetting have completely different structure.In addition, TiO2It is a kind of weight
The functional material wanted, has in ultraviolet irradiation, produces the key property of Photogenerated Radicals.On the other hand, since it is good
Corrosion resistance and mechanical property, TiO2In technical field of biological material, such as bone implant material, contacting blood material, biology sensor
Field has a wide range of applications.
Therefore, the method for the embodiment of the present invention is in TiO2After adsorption albumen, respectively in dry environment and water (moistening
Environment) in carry out ultraviolet irradiation, utilize TiO2Photogenerated Radicals caused by ultraviolet irradiation carry out albumen that is dry or soaking
It is covalently fixed, in addition, the environmental condition (dry or wetting) during by adjusting ultraviolet irradiation, to regulate and control the cell of ankyrin parent
And property, i.e., it is a kind of to utilize dry/wet ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility.
The method of the embodiment of the present invention is suitable for multiple protein (including albumin, fibrinogen, collagen etc.) and exists
Various forms TiO2Material (including film, the nanotube, nano particle etc.) fixation on surface and the regulation and control of cellular affinity, and
Surface or the preparation of cell micrographics.Ultraviolet irradiation process is simple controllable with environmental condition, relative to existing method, has suitable
With scope is wide, controllability is good, technique is simple, economic the advantages of facilitating.
The embodiment of the present invention also provides a kind of TiO2- protein product, it is according to described above using ultraviolet irradiation in TiO2
The method of upper ankyrin and regulating cell compatibility is made.If ultraviolet irradiation is full ultraviolet irradiation, corresponding TiO2- albumen produces
Product is promotions or suppress follow-up albumen, the protein surface that cell adsorbs, i.e. TiO2- albumen composite surface；If ultraviolet irradiation is to cover
Film ultraviolet irradiation, corresponding TiO2- protein product is promotion or the micro-patterning albumen of suppression cell adhesion protein-general proteins
Surface, i.e. TiO2- albuminous cell micrographics surface.TiO2- protein product is specifically in TiO2More hatching eggs are effectively fixed on surface
In vain, and obtained from freely regulating and controlling the cellular affinity of multiple protein, the TiO of acquisition2- albumen composite surface and TiO2- albumen is thin
Born of the same parents' micrographics surface can meet different biomaterial application fields (such as Integrated implant material, contacting blood material) to material biology
The individual demand of performance is learned, there is important practical value.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of TiO2- bovine albumin composite surface, it is specifically made in accordance with the following methods：
By TiO2Material is placed in bovine albumin solution, makes TiO2Adsorption bovine albumin, takes out and is cleaned with RO water,
Wash unadsorbed bovine albumin off, obtain TiO2- bovine albumin sample.
By the TiO of moistening2- bovine albumin sample carries out the full ultraviolet irradiation of wet method in water, and obtaining, there is resistance cell to glue
The TiO of attached ability2- bovine albumin composite surface.
Embodiment 2
The present embodiment provides a kind of TiO2- bovine albumin composite surface, it is specifically made in accordance with the following methods：
By TiO2Material is placed in bovine albumin solution, makes TiO2Adsorption bovine albumin, it is white and clear with RO water to take out ox
Wash, wash unadsorbed bovine albumin off, obtain TiO2- bovine albumin sample.
By TiO2- bovine albumin sample spontaneously dries in atmosphere.
By dried TiO2- bovine albumin sample carries out the full ultraviolet irradiation of dry method in dry environment, obtains with good
The TiO of good cellular affinity2- bovine albumin composite surface.
Embodiment 3
The present embodiment provides a kind of TiO2- bovine albumin cell micrographics, it is specifically made in accordance with the following methods：
By TiO2Material is placed in bovine albumin solution, makes TiO2Adsorption bovine albumin, it is white and clear with RO water to take out ox
Wash, wash unadsorbed bovine albumin off, obtain TiO2- bovine albumin sample.
By the TiO of moistening2- bovine albumin sample carries out wet method mask ultraviolet irradiation in water, and obtaining has resistance cell
The TiO of adhesive capacity2- albuminous cell micrographics surface.
Embodiment 4
The present embodiment provides a kind of TiO2- bovine albumin cell micrographics, it is specifically made in accordance with the following methods：
By TiO2Material is placed in bovine albumin solution, makes TiO2Adsorption bovine albumin, it is white and clear with RO water to take out ox
Wash, wash unadsorbed bovine albumin off, obtain TiO2- bovine albumin sample.
By TiO2- bovine albumin sample spontaneously dries in atmosphere.
By dried TiO2- bovine albumin sample carries out dry method mask ultraviolet irradiation in dry environment, is had
The TiO of good cellular affinity2- albuminous cell micrographics surface.
Embodiment 5
The present embodiment provides two kinds of TiO2- fibrin archaeocyte micrographics, it is specifically made in accordance with the following methods：
By TiO2Material is placed in fibrinogen solution, makes TiO2Adsorption fibrinogen, takes out fiber and uses RO
Water cleans, and washes unadsorbed fibrinogen off, obtains TiO2- fibrinogen sample.
By TiO2- fibrinogen sample spontaneously dries in atmosphere.
By dried TiO2- fibrinogen sample carries out dry method mask ultraviolet irradiation in dry environment, is had
There is a kind of TiO of good cellular affinity2- fibrin archaeocyte micrographics.
In addition, by dried TiO2- fibrinogen sample soaks again, carries out the ultraviolet spoke of wet method mask in water
According to TiO of the acquisition with resistance cell adherence ability2- fibrin archaeocyte micrographics.
Below to the TiO in embodiment 1-52The cellular affinity result of-protein product is detected.
First, Fig. 1 regulates and controls TiO for embodiment 1-4 using wet method/dry method ultraviolet irradiation2Surface protein cellular affinity is illustrated
1. figure, Fig. 1 regulate and control TiO for embodiment 1 using the full ultraviolet irradiation of wet method22. surface protein cellular affinity schematic diagram, Fig. 1 are
Embodiment 2 utilizes the full ultraviolet irradiation regulation and control TiO of dry method23. surface protein cellular affinity schematic diagram, Fig. 1 utilize for embodiment 3
Wet method mask ultraviolet irradiation regulates and controls TiO2Surface protein cellular affinity schematic diagram, Fig. 1 are 4. purple using dry method mask for embodiment 4
External irradiation regulates and controls TiO2Surface protein cellular affinity schematic diagram, wherein, BSA=bovine albumins, UV (W)=wet method ultraviolet spoke entirely
According to, UV (the D)=full ultraviolet irradiation of dry method, PUV (W)=wet method mask ultraviolet irradiation, PUV (D)=dry method mask ultraviolet irradiation,
Mask=masks, Transparent Section=transparent parts.
Respectively in the TiO of embodiment 1, embodiment 22- bovine albumin composite surface repopulating cell, repopulating cell are thin for endothelium
Born of the same parents ECs, incubation time are 1 day, and Fig. 2 represents embodiment 1 using the full ultraviolet irradiation regulation and control TiO of wet method2Surface protein cell is affine
Property as a result, Fig. 3 represents embodiment 2 using dry method full ultraviolet irradiation regulation and control TiO2Surface protein cellular affinity result.
It can be seen that by Fig. 2, Fig. 3：Regulate and control TiO using wet method ultraviolet irradiation2Surface protein has resistance cell adherence energy
Power, cellular affinity is relatively low, regulates and controls TiO using dry method ultraviolet irradiation2Surface protein has preferable cellular affinity.
2nd, Fig. 4 regulates and controls TiO for embodiment 5 using wet method/dry method mask ultraviolet irradiation2Surface protein cellular affinity shows
It is intended to.
Respectively by two kinds of TiO in embodiment 52The surface grafting cell of-fibrin archaeocyte micrographics, repopulating cell
For endothelial cell ECs, incubation time is 1 day, and Fig. 5 represents embodiment 5 using dry/wet mask ultraviolet irradiation regulation and control TiO2Table
Face albuminous cell compatibility as a result, FGN-PUV (W)=fibrinogen passes through wet method mask ultraviolet irradiation, FGN-PUV (D)=
Fibrinogen passes through dry method mask ultraviolet irradiation.
As seen from Figure 5：Regulate and control TiO using wet method ultraviolet irradiation2Surface protein has resistance cell adherence ability, profit
Regulate and control TiO with dry method ultraviolet irradiation2Surface protein has preferable cellular affinity.
In conclusion the embodiment of the present invention utilizes ultraviolet irradiation in TiO2Upper ankyrin and regulating cell compatibility
Method can be with Effective Regulation TiO2The cellular affinity of adsorption albumen or the means irradiated by mask, it is micro- to obtain cell
Figure；Obtained TiO2- protein product can meet personalized need of the different biomaterial application fields to material biology performance
Ask, there is important practical value.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. one kind is using ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, it is characterised in that it includes
Following steps：
By TiO2Material is placed in protein solution, makes TiO2Adsorption albumen, takes out and washes with water, and washes unadsorbed egg off
In vain, TiO is obtained2- protein sample；
By TiO2- protein sample carries out the ultraviolet irradiation of dry or wet, obtains the TiO with different cellular affinities2- albumen
Product.
2. according to claim 1 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, the TiO2Material is TiO2Film, TiO2Particle or TiO2Nanotube.
3. according to claim 1 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, the albumen includes albumin, fibrinogen, collagen, catalase or superoxide anion mutase.
4. according to claim 1 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, water used is pure water or the salting liquid of water.
5. according to claim 1 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, the ultraviolet irradiation is full ultraviolet irradiation or mask ultraviolet irradiation.
6. according to claim 1 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, the specific method of the ultraviolet irradiation of dry method is：First by the TiO2- protein sample is dried, then in dry environment
Carry out ultraviolet irradiation.
7. according to claim 6 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, dry method is to spontaneously dry or be freeze-dried in atmosphere.
8. according to claim 1 utilize ultraviolet irradiation in TiO2The method of upper ankyrin and regulating cell compatibility, its
It is characterized in that, the specific method of the ultraviolet irradiation of wet method is：By the TiO of moistening2- protein sample carries out ultraviolet spoke in water
According to.
A kind of 9. TiO2- protein product, it is characterised in that it is using purple according to such as described in any item of the claim 1 to 8
External irradiation is in TiO2The method of upper ankyrin and regulating cell compatibility is made.
10. TiO according to claim 92- protein product, it is characterised in that the ultraviolet irradiation is full ultraviolet irradiation, right
The TiO answered2- protein product is promotion or suppresses follow-up albumen, the protein surface that cell adsorbs；The ultraviolet irradiation is purple for mask
External irradiation, corresponding TiO2- protein product is promotion or the micro-patterning albumen table of suppression cell adhesion protein-general proteins
Face.
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| CN201711167358.3A CN107936085A (en) | 2017-11-21 | 2017-11-21 | Using ultraviolet irradiation in TiO2The method and TiO of upper ankyrin and regulating cell compatibility2Protein product |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711167358.3A CN107936085A (en) | 2017-11-21 | 2017-11-21 | Using ultraviolet irradiation in TiO2The method and TiO of upper ankyrin and regulating cell compatibility2Protein product |
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Cited By (1)
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| CN110373407A (en) * | 2019-07-31 | 2019-10-25 | 西南交通大学 | Utilize method, Biofunctional materials and the application of the fixed catalase of photocatalysis |
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Application publication date: 20180420 |