CN107935751A - A kind of microbial-bacterial fertilizer for preventing root-knot nematode and preparation method thereof - Google Patents
A kind of microbial-bacterial fertilizer for preventing root-knot nematode and preparation method thereof Download PDFInfo
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- CN107935751A CN107935751A CN201711496669.4A CN201711496669A CN107935751A CN 107935751 A CN107935751 A CN 107935751A CN 201711496669 A CN201711496669 A CN 201711496669A CN 107935751 A CN107935751 A CN 107935751A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The present invention provides a kind of microbial-bacterial fertilizer for preventing root-knot nematode and preparation method thereof, the raw material for preventing the microbial-bacterial fertilizer of root-knot nematode is mainly：Mare Humorum taro leaf powder, bagasse, calcium monohydrogen phosphate, magnesium sulfate, Ka Wuer streptomycetes etc. form；By the microbial-bacterial fertilizer of prevention root-knot nematode produced by the invention, it is low with production cost, it is environmental-friendly, the characteristics of energy consumption is low, product effectively combines the function of plant prevention root-knot nematode and microbial metabolic products prevention root-knot nematode, soil environment quality can be obviously improved, improves crop yield, and a variety of root-knot nematodes can be prevented.The very low Alocasia ordora leaf powder with obvious acid of utilization rate is efficiently make use of at the same time, is of great significance to local economy with environmental protection.
Description
Technical field
The invention belongs to microbial manure and microbial pesticide technical field, is specifically related to a kind of prevention root-knot nematode
Microbial-bacterial fertilizer and preparation method thereof.
Background technology
Root-knot nematode is one of soil-borne disease for seriously endangering plant, it forms substantial amounts of root knot after infecting root system, and
Phenomena such as plant performance grows short and small and yellowing leaf, causes absorption of the plant to moisture and nutrient difficult, seriously affects plant
Normal growth and development.Therefore, root-knot nematode causes crop huge yield and economic loss.Continuous cropping is to cause
The principal element that plant root-knot nematodes evil occurs, but since root-knot nematode host range is very extensive, including the overwhelming majority
Crop and weeds, prevent once being difficult to other approach by crop rotation etc..At present, root-knot nematode is prevented in agricultural production
Method be mainly chemical prevention, but many negative impacts are caused to environment and mankind itself, and many countries or
Area has carried out stringent limitation to the chemicals for preventing root-knot nematode into market.Therefore, find new, environmental-friendly
The biological control method of type is very urgent and necessary.
Alocasia ordora [Alocasia macrorrhiza (L.) Schott] is Araeceae Alocasia ordora platymiscium, according to《Soil pesticide and
Biological control》Record, Alocasia ordora water extract works well prevention rice plant skipper, mythimna separata, planthopper, rice leafhopper.Hou Xuewen etc. from
Alocasia ordora agglutinin (Alocasia macrorrhiza lectin, AML) is isolated in Alocasia ordora stem tuber, and finds Alocasia ordora agglutinin
There is stronger direct killing ability to lung adenocarcinoma cell line P18.Great mass of data proves at the same time, from a variety of aggegations of plant
Plain (GNA, WGA, ConA etc.) shows obvious insecticidal activity, and has been applied in insect-resistant transgenic plants.But do not report
Alocasia ordora agglutinin is applied on prevention root-knot nematode by road.Also Alocasia ordora leaf is not applied on prevention root-knot nematode.
The content of the invention
The object of the present invention is to provide a kind of microbial-bacterial fertilizer for preventing root-knot nematode, the microbial bacteria of root-knot nematode is prevented
Fertilizer raw material be mainly：Mare Humorum taro leaf powder, bagasse, calcium monohydrogen phosphate, magnesium sulfate, Ka Wuer streptomycetes etc. form；The present invention is raw
The microbial-bacterial fertilizer of the prevention root-knot nematode of production, effectively combines plant prevention worm and microbial metabolic products prevention root-knot nematode
Function, can be obviously improved soil environment quality, improve crop yield, have that production cost is low, environmental-friendly, the low spy of energy consumption
Point, product efficiently make use of the very low Alocasia ordora leaf powder with obvious acid of utilization rate at the same time, to local economy and environmental protection
It is of great significance.
In order to achieve the above object, the present invention is by weight using the raw material of sisal dregs production Trichoderma harzianum bacterial manure：It is wet
800-900 parts of Alocasia ordora leaf powder, 50-100 parts of bagasse, 1-2 parts of calcium monohydrogen phosphate, 0.1-0.2 parts of magnesium sulfate, Ka Wuer streptomycetes
The compositions such as 10-20 parts.
It is including as follows it is a further object of the present invention to provide a kind of preparation method for the microbial-bacterial fertilizer for preventing root-knot nematode
Step：
(1) the 800-900 parts of Mare Humorum that crushed 2 mm sieves taro leaf powder, are sequentially added into rotation cooker, add bagasse 50-
100 parts, 1-2 parts of calcium monohydrogen phosphate, 0.1-0.2 parts of magnesium sulfate, stirs evenly, and 121 DEG C of sterilizing 30-50min, are cooled to 30 DEG C；
(2), 10-20 part Ka Wuer streptomycete seeds are added to the fermentation materials that have cooled down of step (1) middle sterilizing, stirring is
It is even, uniform fermentation materials are laid in pallet or fermentation tank, material thickness 5-8cm, are 55%-75% in humidity, temperature
For the 72-96h that ferments in 28-32 DEG C of fermenting cellar, treat that fermentation materials are covered with white thalline, detect its Ka Wuer strepto- spore content
More than 10,000,000,000 CFU/g, you can to terminate to ferment；
(3) fermentate low temperature drying to water content, is less than 13%, you can crushing packing；
Wherein, the preparation method of Ka Wuer streptomycetes seed is as follows：
Culture presevation pipe clamp Wu Er streptomycetes ACCC 41145 (buying in Chinese agriculture Culture Collection) is taken out, is used
Gause I solid medium draws tablet and recovers, and 30 DEG C are cultivated 4-5 days.Picking single bacterium colony is seeded to eggplant under tablet
In bottle on Gause I solid medium, whole eggplant bottle is filled, cultivates 4-6 days for 30 DEG C in the incubator, waits phage surface to have
Baby pink spore, 1L sterile waters are washed down the lawn in eggplant bottle, the Ka Wuer streptomycetes seed inoculation under washing
In the 500L seed fermentation tanks equipped with 350L Ka Wuer streptomycete fluid nutrient mediums, initial pH value 7.2, temperature 30
DEG C, ventilation 1:1, open stirring, rotating speed 200r/min；When culture 36-48 is small, treat that thalline becomes viscous, there are a large amount of mycelia to produce,
It can be used as Ka Wuer streptomycete seeds；
Wherein, the Gause I culture medium：Starch 20g, NaCl 0.5g, KNO3 1g, KH2PO43H2O 0.5g,
FeSO47H2O0.01g, MgSO47H2O 0.5g, agar 18g, water 1000ml；
Wherein, the Ka Wuer streptomycetes fluid nutrient medium：20 g/L of glucose, 20 g/L of bean cake powder, 0.5 g/L of magnesium sulfate,
Calcium carbonate 2 g/L, pH7.2；The condition of each medium sterilization is：0.10-0.15MPa, 121 DEG C sterilize 30 minutes.
Beneficial effects of the present invention：The microbial-bacterial fertilizer of prevention root-knot nematode produced by the invention, effectively combines plant
The function of worm and microbial metabolic products prevention root-knot nematode is prevented, soil environment quality can be obviously improved, improve crop yield,
It is low with production cost, environmental-friendly, the characteristics of energy consumption is low, product efficiently make use of at the same time utilization rate it is very low have it is obvious pungent
The Alocasia ordora leaf powder of pungent, local economy is of great significance with environmental protection.
Embodiment
Embodiment 1
A kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：Mare Humorum
850 parts of taro leaf powder, 100 parts of bagasse, 1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, 20 parts of Ka Wuer streptomycetes etc. form.
Wherein, the preparation method of the microbial-bacterial fertilizer of a kind of prevention root-knot nematode, includes the following steps：
(1) the 850 parts of Mare Humorum that crushed 2 mm sieves taro leaf powder, are sequentially added into rotation cooker, add 100 parts of bagasse,
1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, stirs evenly, and 121 DEG C of sterilizing 30-50min, are cooled to 30 DEG C；
(2) 20 parts of Ka Wuer streptomycete seeds, are added to the fermentation materials that have cooled down of step (1) middle sterilizing, are stirred evenly, will
Uniform fermentation materials are laid in pallet or fermentation tank, and material thickness 5-8cm, is 55%-75%, temperature 28- in humidity
Ferment 84h in 32 DEG C of fermenting cellar, and fermentation materials are covered with white thalline, detects its Ka Wuer strepto-s spore content as 11,200,000,000
CFU/g, that is, terminate to ferment；
(3) it is, 10% by fermentate low temperature drying to water content, crushing packing；
Embodiment 2
A kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：Mare Humorum
900 parts of taro leaf powder, 80 parts of bagasse, 1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, 20 parts of Ka Wuer streptomycetes etc. form.
Wherein, the preparation method of the microbial-bacterial fertilizer of a kind of prevention root-knot nematode, includes the following steps：
(1) the 900 parts of Mare Humorum that crushed 2 mm sieves taro leaf powder, are sequentially added into rotation cooker, add 80 parts of bagasse, phosphorus
Sour 1 part of hydrogen calcium, 0.1 part of magnesium sulfate, stirs evenly, and 121 DEG C of sterilizing 30-50min, are cooled to 30 DEG C；
(2) 20 parts of Ka Wuer streptomycete seeds, are added to the fermentation materials that have cooled down of step (1) middle sterilizing, are stirred evenly, will
Uniform fermentation materials are laid in pallet or fermentation tank, and material thickness 5-8cm, is 55%-75%, temperature 28- in humidity
Ferment 88h in 32 DEG C of fermenting cellar, and fermentation materials are covered with white thalline, detects its Ka Wuer strepto-s spore content as 10,800,000,000
CFU/g, that is, terminate to ferment；
(3) it is, 10% by fermentate low temperature drying to water content, crushing packing.
Comparative example 1（It is ferment-fermented with the common bacterial manure in market）
A kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：Mare Humorum
850 parts of taro leaf powder, 100 parts of bagasse, 1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, 20 parts of bacterial manure leavening purchased in market etc. form；It is other
With embodiment 1.
Comparative example 2（The Streptomyces jingyangensis fermentation test acted on common disease prevention growth-promoting）
A kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：Mare Humorum
900 parts of taro leaf powder, 80 parts of bagasse, 1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, 20 parts of Streptomyces jingyangensis etc. form；It is other same real
Apply example 2.
Comparative example 3（Convenient source ferments, and Alocasia ordora leaf powder is substituted with sheep manure）
A kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：Sheep manure
850 parts, 100 parts of bagasse, 1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, the composition such as 20 parts of Ka Wuer streptomycetes；Other same embodiments
1。
Comparative example 4（Alocasia ordora leaf powder is substituted with wheat bran to ferment）
A kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：Wheat bran
900 parts, 80 parts of bagasse, 1 part of calcium monohydrogen phosphate, 0.1 part of magnesium sulfate, the composition such as 20 parts of Streptomyces jingyangensis；The other the same as in Example 2.
Embodiment 3:Prevent application of the microbial-bacterial fertilizer of root-knot nematode on tomato
Experimental group：
Implementation group 1：100 times of dilutions when tomato is transplanted respectively in the microbial-bacterial fertilizer produced with embodiment 1 carry out being stained with root,
And carry out filling 5 kgs/acre of microbial-bacterial fertilizer for applying the production of embodiment 1；
Implementation group 2：100 times of dilutions when tomato is transplanted respectively in the microbial-bacterial fertilizer produced with embodiment 2 carry out being stained with root,
And carry out filling 5 kgs/acre of microbial-bacterial fertilizer for applying the production of embodiment 2；
Contrast groups 1：100 times of dilutions when tomato is transplanted respectively in the microbial-bacterial fertilizer produced with comparative example 1 carry out being stained with root,
And carry out filling 5 kgs/acre of microbial-bacterial fertilizer for applying the production of comparative example 1；
Contrast groups 2：100 times of dilutions when tomato is transplanted respectively in the microbial-bacterial fertilizer produced with comparative example 2 carry out being stained with root,
And carry out filling 5 kgs/acre of microbial-bacterial fertilizer for applying the production of comparative example 2；
Contrast groups 3：100 times of dilutions when tomato is transplanted respectively in the microbial-bacterial fertilizer produced with comparative example 3 carry out being stained with root,
And carry out filling 5 kgs/acre of microbial-bacterial fertilizer for applying the production of comparative example 3；
Contrast groups 4：100 times of dilutions when tomato is transplanted respectively in the microbial-bacterial fertilizer produced with comparative example 4 carry out being stained with root,
And carry out filling 5 kgs/acre of microbial-bacterial fertilizer for applying the production of comparative example 4；
Control group 1：Root clear water is stained with, filling, which is applied, also only uses clear water；
Control group 2：The use of conventional pesticide main ingredient to be avermectin, 1000 times of progress pouring root uses are diluted, every 15 days
Using once, use altogether twice；It is transplanted to the serious untreated tomato seedling of field of a piece of former years root knot nematode disease morbidity
As control, it is managed according to a conventional method.
As a result：Tomato investigates tomato plant root system root knot nematode disease incidence, investigation result such as table 1 at the end of picking：
According to the microbial-bacterial fertilizer of embodiment production, its prevention effect reaches more than 92%, and uses normal fermentation agent fermentation Alocasia ordora leaf powder
's（Contrast groups 1 and contrast groups 2）Its prevention effect of obtained microbial inoculum is only between 52.39%-56.76%；And use card 5
During your other matrix of streptomycete fermentation（Contrast groups 3-4）Its prevention effect also only only has between 59.16%-62.38%；Contrast groups
The effect of the prevention root-knot nematode of 1-4 is good using the effect of avermectin not as control group 2, and embodiment 1 is used with embodiment 2
Effect then far better than control group use avermectin effect.
1 each group root-knot nematode incidence of table
| Group | Bacterial manure species | Disease index（%） | Preventive effect（%） |
| Control group 1 | Clear water | 96.34 | |
| Embodiment 1 | The microbial-bacterial fertilizer that embodiment 1 produces | 7.12 | 92.61 |
| Embodiment 2 | The microbial-bacterial fertilizer that embodiment 2 produces | 6.89 | 92.85 |
| Contrast 1 | The microbial-bacterial fertilizer that comparative example 1 produces | 45.87 | 52.39 |
| Contrast 2 | The microbial-bacterial fertilizer that comparative example 2 produces | 41.66 | 56.76 |
| Contrast 3 | The microbial-bacterial fertilizer that comparative example 3 produces | 36.24 | 62.38 |
| Contrast 4 | The microbial-bacterial fertilizer that comparative example 4 produces | 39.35 | 59.16 |
| Control group 2 | Avermectin | 28.19 | 70.74 |
Embodiment 4:Prevent application of the microbial-bacterial fertilizer of root-knot nematode on banana.
Tested with falling ill serious banana with a piece of root knot nematode disease, test group：Mu is applied micro- before banana budding
10 kg of bio-bacterial manure（Every plant is applied 0.08kg- 0.1kg）；Control group is conventional avermectin, dilutes 1000 times of progress pouring roots
Use, every 15 days using once, use altogether twice；It is managed according to a conventional method.Banana investigates banana at the end of picking
Plant root root knot nematode disease incidence, investigation result are shown:In banana using microbial-bacterial fertilizer relative to conventional Avermectin
The relative control effect of element improves more than 67%, and yield and the control group of banana improve 45%.
Embodiment 5:Prevent application of the microbial-bacterial fertilizer of root-knot nematode on fertile mandarin orange
Tested with falling ill serious fertile mandarin orange with a piece of root knot nematode disease, test group：Mu applies microorganism before fertile mandarin orange blooms
20 kg of bacterial manure（Every plant is applied 0.2kg- 0.25kg）；Control group is conventional avermectin, dilutes 1000 times of progress pouring root uses,
Every 15 days using once, use altogether twice；It is managed according to a conventional method.Fertile mandarin orange plant root is investigated at the end of fertile mandarin orange picking
It is root knot nematode disease incidence, investigation result is shown：Phase in fertile mandarin orange using microbial-bacterial fertilizer relative to conventional avermectin
More than 42% is improved to preventive effect, the yield of fertile mandarin orange improves 23% with control group.Actinomyces in test group soil compare control group
In actinomyces improve 290%, fungal content reduces 64%, while test group microbial-bacterial fertilizer also declines the soil weight,
Cation exchange capacity increase, total soil nitrogen, full phosphorus, available nitrogen, available potassium increase, so as to improve the structure and nutrition shape of soil
Condition, repair is played to soil, makes yield increase obvious.
Claims (2)
1. a kind of microbial-bacterial fertilizer for preventing root-knot nematode, it is characterised in that the raw material of the microbial-bacterial fertilizer is by weight：It is wet
800-900 parts of Alocasia ordora leaf powder, 50-100 parts of bagasse, 1-2 parts of calcium monohydrogen phosphate, 0.1-0.2 parts of magnesium sulfate, Ka Wuer streptomycetes
The compositions such as 10-20 parts.
A kind of 2. preparation method of microbial-bacterial fertilizer for preventing root-knot nematode according to claim 1, it is characterised in that bag
Include following steps：
(1) the 800-900 parts of Mare Humorum that crushed 2 mm sieves taro leaf powder, are sequentially added into rotation cooker, add bagasse 50-
100 parts, 1-2 parts of calcium monohydrogen phosphate, 0.1-0.2 parts of magnesium sulfate, stirs evenly, and 121 DEG C of sterilizing 30-50min, are cooled to 30 DEG C；
(2), 10-20 part Ka Wuer streptomycete seeds are added to the fermentation materials that have cooled down of step (1) middle sterilizing, stirring is
It is even, uniform fermentation materials are laid in pallet or fermentation tank, material thickness 5-8cm, are 55%-75% in humidity, temperature
For the 72-96h that ferments in 28-32 DEG C of fermenting cellar, treat that fermentation materials are covered with white thalline, detect its Ka Wuer strepto- spore content
More than 10,000,000,000 CFU/g, you can to terminate to ferment；
(3) fermentate low temperature drying to water content, is less than 13%, you can crushing packing；
Wherein, Ka Wuer streptomycetes seed is prepared as follows：
Culture presevation pipe is taken out, tablet is drawn with Gause I solid medium and recovers, 30 DEG C are cultivated 4-5 days, under tablet
Picking single bacterium colony is seeded in eggplant bottle on Gause I solid medium, fills whole eggplant bottle, 30 DEG C in the incubator
Culture 4-6 days, waits phage surface to have and is covered with spore, 1L sterile waters are washed down the lawn in eggplant bottle, by the card under washing
Wu Er streptomycetes are inoculated in the 500L seed fermentation tanks equipped with 350L Ka Wuer streptomycete fluid nutrient mediums, and initial pH value is
7.2, temperature is 30 DEG C, ventilation 1:1, open stirring, rotating speed 200r/min；When culture 36-48 is small, treats that thalline becomes viscous, have
A large amount of mycelia produce, you can as Ka Wuer streptomycete seeds；
Wherein, the Gause I culture medium：Starch 20g, NaCl 0.5g, KNO3 1g, KH2PO43H2O 0.5g,
FeSO47H2O0.01g, MgSO47H2O 0.5g, agar 18g, water 1000ml；
Wherein, the Ka Wuer streptomycetes fluid nutrient medium：20 g/L of glucose, 20 g/L of bean cake powder, 0.5 g/L of magnesium sulfate,
Calcium carbonate 2 g/L, pH7.2；The condition of each medium sterilization is：0.10-0.15MPa, 121 DEG C sterilize 30 minutes.
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| CN201711496669.4A CN107935751A (en) | 2017-12-31 | 2017-12-31 | A kind of microbial-bacterial fertilizer for preventing root-knot nematode and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113789274A (en) * | 2021-08-19 | 2021-12-14 | 东莞市农业科学研究中心 | Grape rhizosphere antagonistic growth-promoting streptomyces F2 and application thereof |
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| CN101790995A (en) * | 2010-02-08 | 2010-08-04 | 广东省农业科学院植物保护研究所 | Biological preparation for prevention and treatment of peanut root-knot nematode, and preparation method and applications thereof |
| CN104016813A (en) * | 2014-06-06 | 2014-09-03 | 茹晖 | Ecological pesticide-added fertilizer and preparation method thereof |
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2017
- 2017-12-31 CN CN201711496669.4A patent/CN107935751A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101790995A (en) * | 2010-02-08 | 2010-08-04 | 广东省农业科学院植物保护研究所 | Biological preparation for prevention and treatment of peanut root-knot nematode, and preparation method and applications thereof |
| CN104016813A (en) * | 2014-06-06 | 2014-09-03 | 茹晖 | Ecological pesticide-added fertilizer and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| 段佳丽，等: "放线菌Actl2与腐植酸钾配施对丹参生长及其根域微生态的影响", 《生态学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113789274A (en) * | 2021-08-19 | 2021-12-14 | 东莞市农业科学研究中心 | Grape rhizosphere antagonistic growth-promoting streptomyces F2 and application thereof |
| CN113789274B (en) * | 2021-08-19 | 2023-02-17 | 东莞市农业科学研究中心 | Grape rhizosphere antagonistic growth-promoting streptomyces F2 and application thereof |
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