CN107929805B - Metal/hydrogel composite dressing for promoting wound healing and preparation method thereof - Google Patents
Metal/hydrogel composite dressing for promoting wound healing and preparation method thereof Download PDFInfo
- Publication number
- CN107929805B CN107929805B CN201810020842.1A CN201810020842A CN107929805B CN 107929805 B CN107929805 B CN 107929805B CN 201810020842 A CN201810020842 A CN 201810020842A CN 107929805 B CN107929805 B CN 107929805B
- Authority
- CN
- China
- Prior art keywords
- metal
- hydrogel
- composite dressing
- wound healing
- hydrogel composite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention provides a preparation method of a metal/hydrogel composite dressing for promoting wound healing, which comprises the following steps: treating the surface of the conductive metal foil or metal powder by a coupling agent; compounding the treated conductive metal foil or metal powder with a hydrogel pre-gel system; and chemically or physically crosslinking the compounded hydrogel pre-gel system to form the metal/hydrogel composite dressing. The dressing prepared by the invention is a flexible conductive material, and can stimulate the whole wound area to promote wound healing, so that the defects that the conventional electrode stimulation therapy is not suitable for large-area wounds and cannot uniformly stimulate the whole wound area are effectively overcome. The hydrogel component in the dressing can also provide a moist environment for the wound, absorb the tissue exudate and further promote wound healing. Therefore, the metal/hydrogel composite dressing has wide application value in the treatment of large-area burns, chronic wounds and the like.
Description
Technical Field
The invention relates to a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof.
Background
Chronic wounds generally refer to wounds that heal for more than 12 weeks or are prone to relapse after healing. Chronic wounds pose a serious problem threatening human health due to their high incidence, high prevalence, and difficulty in effective treatment. Even in such developed countries in the united states, patients with chronic wounds can reach as high as 650 million, with costs in excess of 250 million dollars per year for chronic wound treatment. With the trend of aging population, the number of patients with chronic wounds will also increase dramatically in the foreseeable future, and the associated costs of treatment and care will also increase year by year. At present, the method for treating chronic wounds in clinic mainly adopts wound dressing and is assisted by other treatment means such as debridement, negative pressure treatment, hyperbaric oxygen treatment and the like. However, clinical practice has shown that the therapeutic effect of this conventional treatment on chronic wounds is still not ideal. Therefore, the development of a safe and effective novel therapeutic approach for chronic wounds has become an important problem to be solved in the field of current medical treatment.
Different kinds of ions are distributed inside and outside human cells, and the transmembrane movement of the ions forms bioelectricity on the cellular level. The instant the epithelial layer of human skin is destroyed, an endogenous electric field is generated and a wound current of less than 1mA is formed at the wound. Wound current plays an important role in wound healing. It has been found that when an applied current of a magnitude comparable to the wound current is applied to the wound, the wound healing is accelerated, and that blocking the conduction of the wound current at the wound results in a delay or cessation of the wound healing process.
Based on the wound current promoting effect on wound healing, the use of electrical stimulation to treat chronic wounds has been studied. Although the mechanism involved is yet to be further defined, it is now well recognized that electrical stimulation has a healing promoting effect on a variety of wounds and different stages of wound healing. So far, no obvious toxic and side effects on human bodies caused by electric stimulation are found in relevant clinical tests, and only slight rash and skin irritation are found to be possibly caused. Except for patients with malignant tumors or with implanted electronic devices, electrical stimulation can be applied to almost all other patients without limitation by factors such as age, sex, etc. At present, the electric stimulation therapy for promoting wound healing is incorporated into medical insurance by a plurality of insurance companies in the United states, and shows good clinical application prospect.
However, current electrical stimulation treatment protocols are all very simple to apply electrical signals directly to the wound using electrodes placed in the vicinity of the wound. Due to the large skin impedance of the human body (about 10M omega/cm)2) This approach is not suitable for large area wounds and does not provide uniform stimulation over the entire wound area.
Disclosure of Invention
The invention aims to provide a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof, and aims to solve the key problems that the existing electrode stimulation therapy cannot be suitable for large-area wounds and cannot perform uniform electrical stimulation on the whole wound area.
In order to achieve the purpose, the invention adopts the following technical scheme:
a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof comprise the following steps:
A. treating the surface of the conductive metal foil or metal powder by a coupling agent;
B. compounding the treated conductive metal foil or metal powder with a hydrogel pre-gel system;
C. and C, chemically or physically crosslinking the hydrogel pre-gel system compounded in the step B to form the metal/hydrogel composite dressing. The dressing uses the metal foil material as a conductive component, can uniformly stimulate the whole wound area, and can overcome the defects of the current electrode therapy. The hydrogel component in the dressing can also provide a moist environment for the wound, absorb the tissue exudate and further promote the wound healing.
Preferably, in the step a, the metal is one or more of titanium, aluminum, magnesium, zinc, copper, silver, platinum or gold.
Preferably, in the step a, the metal is a metal element or a bioinert metal naturally contained in a human body.
Preferably, in the step B, before the metal-hydrogel composite is prepared, a reactive or polar group is introduced on the metal surface. The existing metal material and the hydrogel material are combined weakly and are easy to separate. In order to realize the preparation of the metal/hydrogel composite dressing, the invention also provides a novel metal surface modification method, and the metal surface is pretreated by using a coupling agent of which the tail end contains a polar group or a double bond, so that the close combination of the metal foil and the hydrogel material can be realized, and the use requirement of the dressing is met.
Preferably, in the step a, the coupling agent is one or more selected from methacryloxysilane coupling agents (e.g., KH 570), vinylsilane coupling agents (e.g., a 171), catechol coupling agents (e.g., dopamine), and vinylthiol coupling agents (e.g., 2-propene-1-thiol).
Preferably, in step B, the hydrogel pre-gel system includes acrylamide monomers (such as acrylamide, etc.), double bond-containing cross-linking agents (such as N, N-methylene bisacrylamide, etc.), water-soluble photo-initiators (such as 2959, etc.) or water-soluble thermal initiators (such as ammonium persulfate), etc.
Preferably, in the step C, the hydrogel pre-gel is crosslinked by ultraviolet light-initiated polymerization, and the reaction time is 10-100 minutes.
Preferably, in the step C, the hydrogel pre-gel is crosslinked by heating to initiate polymerization, wherein the reaction temperature is 40-90 ℃ and the reaction time is 1-20 hours.
Preferably, in the step C, unreacted monomers and initiators are removed by soaking for 3-10 days.
Due to the adoption of the technical scheme, the invention has the following beneficial effects:
1. the invention has wide application range, does not need to use a metal material with special crystal form and ultra-pure, and reduces the production cost.
2. The method has the advantages of simple process, easy operation, no use of organic solvent and green and environment-friendly process flow.
3. The metal/hydrogel conductive dressing prepared by the invention can electrically stimulate the whole wound area, can effectively overcome the problems that the current electrode therapy is not suitable for large-area wounds and can not uniformly stimulate the whole wound, and can effectively promote the wound healing.
4. The metal/hydrogel conductive dressing prepared by the invention can provide a moist environment for a wound while electrically stimulating, so that the wound healing is further promoted.
5. The metal/hydrogel conductive dressing prepared by the invention can be suitable for chronic wounds which are difficult to cure in the prior art, such as diabetic ulcer, traumatic ulcer, pressure ulcer and the like.
Drawings
FIG. 1 is a schematic diagram of the structure of a foil/hydrogel composite dressing of the present invention.
Fig. 2 is a graph showing the cytotoxicity results of the aluminum foil/hydrogel composite dressing.
Fig. 3 is a graph of the results of the wound healing of rats with the aluminum foil/hydrogel composite dressing.
Fig. 4 is a schematic structural diagram of a metal powder/hydrogel composite dressing.
Fig. 5 is a graph showing cytotoxicity results of the iron powder/hydrogel composite dressing.
Fig. 6 is a graph showing the cytotoxicity results of the gold foil/hydrogel composite dressing.
Fig. 7 is a graph of the results of wound healing in rats with gold foil/hydrogel composite dressing.
FIG. 8 is a graph showing the cytotoxicity results of the copper powder/hydrogel composite dressing.
Fig. 9 is a graph showing the cytotoxicity results of the silver foil/hydrogel composite dressing.
Fig. 10 is a graph of the results of wound healing in rats with silver foil/hydrogel composite dressing.
Reference numerals: 1 is hydrogel, 2 is metal foil, 3 is lead, and 4 is metal powder.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1: a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof comprise the following steps:
1) 20 wt% acrylamide, 1 wt% N, N-methylene bisacrylamide and 0.1 wt% photoinitiator 2959 were dissolved in PBS solution to prepare a hydrogel pre-gel system.
2) Cutting the aluminum foil into strip-shaped samples, and ultrasonically cleaning the samples for more than 30 minutes by using dichloromethane, acetone, ethanol and deionized water in sequence to remove dirt on the surfaces.
3) Coating a layer of 1 wt% KH570 solution on the surface of the clean aluminum strip obtained in the step 2), and reacting for 1 hour. After the reaction is finished, the aluminum strip with the treated surface is placed into a mold.
4) Adding the hydrogel pre-gel prepared in the step 1) into the mould described in the step 3), and crosslinking for 20 minutes under ultraviolet light to prepare the metal/hydrogel composite dressing.
5) The metal/hydrogel composite dressing was soaked in sterile PBS solution for 3-10 days to remove unreacted monomers and initiators.
A schematic diagram of the prepared metal/hydrogel composite dressing is shown in fig. 1.
After the conductive hydrogel is prepared, the cytotoxicity of the conductive hydrogel is tested by adopting an in vitro cell experiment. Mouse fibroblast cells L929 at 5X 105Per cm2The cells were grown in 24-well plates and cultured for 24 hours. Cytotoxicity test the cytotoxicity test was carried out using the leach liquor method: the prepared conductive hydrogel was soaked in DMEM cell culture solution (containing 10% fetal bovine serum, 10mg/ml streptomycin and 10000U penicillin) for 24 hours. The cells were further cultured for 24 hours using the extract solution, and the control cells were cultured using DMEM cell culture solution. Cytotoxicity was determined using the MTT method. As shown in fig. 2, there is no significant difference in cell activity between the leaching solution group and the control group, indicating that the metal/hydrogel composite dressing has no cytotoxicity.
Then, the metal/hydrogel composite dressing prepared by taking a diabetic rat as an animal model is tested to have the effect of promoting the healing of the chronic wound. SD rats (body weight 200g or so) were used for the experiment and streptozotocin (45 mg/kg per day) was injected into the tail vein until the fasting blood glucose value of the rats was higher than 16.7 mmol/l. Two wounds (1cm × 1cm) were cut on both sides of the spine of the rat back, and the two wounds were divided into an electrode stimulation group and a dressing electrical stimulation group. The electrode stimulation group used electrodes to electrically stimulate the wound and the dressing electrical stimulation group used a metal/hydrogel conductive dressing to electrically stimulate the wound. The DC voltage of 0.1mA was applied for 1 hour per day. Wound area was determined using a transparency calculating paper. As shown in fig. 3, the promotion of wound healing was more pronounced with electrical stimulation using the metal/hydrogel conductive dressing than with electrical stimulation using electrodes.
Example 2: a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof comprise the following steps:
1) 15 wt% of acrylamide, 3 wt% of N, N-methylene bisacrylamide and 3 wt% of ammonium persulfate are dissolved in the PBS solution to prepare a hydrogel pre-gel system.
2) And ultrasonically cleaning the iron powder for 30 minutes by using dichloromethane, acetone, ethanol and deionized water in sequence to remove the dirt on the surface.
3) The cleaned iron powder is put into a 2 wt% A171 solution and reacted for 0.5-5 hours. Centrifuging the reaction solution to obtain the surface activated iron powder
4) Adding 10 wt% of iron powder into the hydrogel pre-gel system obtained in the step 1).
5) Heating to 60 ℃, and reacting for 10 hours to prepare the metal/hydrogel composite dressing.
6) The metal/hydrogel composite dressing was soaked in sterile PBS solution for 3-10 days to remove unreacted monomers and initiators.
A schematic diagram of the prepared metal/hydrogel composite dressing is shown in fig. 4.
The conductivity of the metal/hydrogel composite dressing was measured using a four-probe tester, and the conductivity of the prepared metal/hydrogel composite dressing was 8S/cm. After the conductive hydrogel is prepared, the cytotoxicity of the conductive hydrogel is tested by adopting an in vitro cell experiment. Mouse fibroblast cells L929 at 5X 105Per cm2The cells were grown in 24-well plates and cultured for 24 hours. Cytotoxicity test the cytotoxicity test was carried out using the leach liquor method: the prepared conductive hydrogel was soaked in DMEM cell culture solution (containing 10% fetal bovine serum, 10mg/ml streptomycin and 10000U penicillin) for 24 hours. The cells were further cultured for 24 hours using the extract solution, and the control cells were cultured using DMEM cell culture solution. Cytotoxicity was determined using the MTT method. As shown in fig. 5, there is no significant difference in cell activity between the leaching solution group and the control group, indicating that the metal/hydrogel composite dressing has no cytotoxicity.
Example 3: a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof comprise the following steps:
1) 25 wt% acrylamide, 5 wt% N, N-methylene bisacrylamide and 4 wt% photoinitiator 2959 were dissolved in PBS solution to prepare a hydrogel pre-gel system.
2) Cutting the gold foil into strip-shaped samples, and ultrasonically cleaning the samples for 30 minutes by using dichloromethane, acetone, ethanol and deionized water in sequence to remove dirt on the surfaces.
3) Coating a layer of 2.5% 2-propylene-1-thiol solution on the surface of the clean gold foil obtained in the step 2), and reacting for 3 hours. After the reaction is finished, the gold foil with the treated surface is placed in a mould.
4) Adding the hydrogel pre-gel prepared in the step 1) into the mould described in the step 3), and crosslinking for 10 minutes under ultraviolet light to prepare the metal/hydrogel composite dressing.
5) The metal/hydrogel composite dressing was soaked in sterile PBS solution for 3-10 days to remove unreacted monomers and initiators.
After the conductive hydrogel is prepared, the cytotoxicity of the conductive hydrogel is tested by adopting an in vitro cell experiment. Mouse fibroblast cells L929 at 5X 105Per cm2The cells were grown in 24-well plates and cultured for 24 hours. Cytotoxicity test the cytotoxicity test was carried out using the leach liquor method: the prepared conductive hydrogel was soaked in DMEM cell culture solution (containing 10% fetal bovine serum, 10mg/ml streptomycin and 10000U penicillin) for 24 hours. The cells were further cultured for 24 hours using the extract solution, and the control cells were cultured using DMEM cell culture solution. Cytotoxicity was determined using the MTT method. As shown in fig. 6, there is no significant difference in cell activity between the leaching solution group and the control group, indicating that the metal/hydrogel composite dressing has no cytotoxicity.
Then, the metal/hydrogel composite dressing prepared by taking a diabetic rat as an animal model is tested to have the effect of promoting the healing of the chronic wound. SD rats (body weight 200g or so) were used for the experiment and streptozotocin (45 mg/kg per day) was injected into the tail vein until the fasting blood glucose value of the rats was higher than 16.7 mmol/l. Two wounds (1cm × 1cm) were cut on both sides of the spine of the rat back, and the two wounds were divided into an electrode stimulation group and a dressing electrical stimulation group. The electrode stimulation group used electrodes to electrically stimulate the wound and the dressing electrical stimulation group used a metal/hydrogel conductive dressing to electrically stimulate the wound. The DC voltage of 0.1mA was applied for 1 hour per day. Wound area was determined using a transparency calculating paper. As shown in fig. 7, the promotion of wound healing was more pronounced with electrical stimulation using the metal/hydrogel conductive dressing than with electrical stimulation using electrodes.
Example 4: a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof comprise the following steps:
1) 20 wt% of acrylamide, 3 wt% of N, N-methylene bisacrylamide and 5 wt% of ammonium persulfate are dissolved in the PBS solution to prepare a hydrogel pre-gel system.
2) And ultrasonically cleaning the copper powder for 30 minutes by using dichloromethane, acetone, ethanol and deionized water in sequence to remove surface dirt.
3) And putting the cleaned copper powder into a dopamine solution with the weight percent of 3% to react for 3 hours. Centrifuging the reaction solution to obtain copper powder with activated surface
4) Adding 10 wt% of copper powder into the hydrogel pre-gel system obtained in the step 1).
5) Heating to 60 ℃, and reacting for 10 hours to prepare the metal/hydrogel composite dressing.
6) The metal/hydrogel composite dressing was soaked in sterile PBS solution for more than 3 days to remove unreacted monomers and initiators.
The conductivity of the metal/hydrogel composite dressing was measured using a four-probe tester, and the conductivity of the prepared metal/hydrogel composite dressing was 10S/cm. After the conductive hydrogel is prepared, the cytotoxicity of the conductive hydrogel is tested by adopting an in vitro cell experiment. Mouse fibroblast cells L929 at 5X 105Per cm2The cells were grown in 24-well plates and cultured for 24 hours. Cytotoxicity test the cytotoxicity test was carried out using the leach liquor method: the prepared conductive hydrogel was soaked in DMEM cell culture solution (containing 10% fetal bovine serum, 10mg/ml streptomycin and 10000U penicillin) for 24 hours. The cells were further cultured for 24 hours using the extract solution, and the control cells were cultured using DMEM cell culture solution. Cytotoxicity was determined using the MTT method. As shown in fig. 8, there is no significant difference in cell activity between the leaching solution group and the control group, indicating that the metal/hydrogel composite dressing has no cytotoxicity.
Example 5: a metal/hydrogel composite dressing for promoting wound healing and a preparation method thereof comprise the following steps:
1) 20 wt% acrylamide, 3 wt% N, N-methylene bisacrylamide and 1 wt% photoinitiator 2959 were dissolved in PBS solution to prepare a hydrogel pre-gel system.
2) Cutting the gold foil into strip-shaped samples, and ultrasonically cleaning the samples for 30 minutes by using dichloromethane, acetone, ethanol and deionized water in sequence to remove dirt on the surfaces.
3) Coating a layer of 2 wt% KH570 solution on the surface of the clean aluminum strip obtained in step 2), and reacting for 4 hours. After the reaction is finished, the aluminum strip with the treated surface is placed into a mold.
4) Adding the hydrogel pre-gel prepared in the step 1) into the mould described in the step 3), and crosslinking for 10 minutes under ultraviolet light to prepare the metal/hydrogel composite dressing.
5) The metal/hydrogel composite dressing was soaked in sterile PBS solution for more than 3 days to remove unreacted monomers and initiators.
After the conductive hydrogel is prepared, the cytotoxicity of the conductive hydrogel is tested by adopting an in vitro cell experiment. Mouse fibroblast cells L929 at 5X 105Per cm2The cells were grown in 24-well plates and cultured for 24 hours. Cytotoxicity test the cytotoxicity test was carried out using the leach liquor method: the prepared conductive hydrogel was soaked in DMEM cell culture solution (containing 10% fetal bovine serum, 10mg/ml streptomycin and 10000U penicillin) for 24 hours. The cells were further cultured for 24 hours using the extract solution, and the control cells were cultured using DMEM cell culture solution. Cytotoxicity was determined using the MTT method. As shown in fig. 9, there is no significant difference in cell activity between the leaching solution group and the control group, indicating that the metal/hydrogel composite dressing has no cytotoxicity.
Then, the metal/hydrogel composite dressing prepared by taking a diabetic rat as an animal model is tested to have the effect of promoting the healing of the chronic wound. SD rats (body weight 200g or so) were used for the experiment and streptozotocin (45 mg/kg per day) was injected into the tail vein until the fasting blood glucose value of the rats was higher than 16.7 mmol/l. Two wounds (1cm × 1cm) were cut on both sides of the spine of the rat back, and the two wounds were divided into an electrode stimulation group and a dressing electrical stimulation group. The electrode stimulation group used electrodes to electrically stimulate the wound and the dressing electrical stimulation group used a metal/hydrogel conductive dressing to electrically stimulate the wound. The DC voltage of 0.1mA was applied for 1 hour per day. Wound area was determined using a transparency calculating paper. As shown in fig. 10, the promotion of wound healing was more pronounced with electrical stimulation using the metal/hydrogel conductive dressing than with electrical stimulation using electrodes.
Claims (7)
1. A preparation method of a metal/hydrogel composite dressing for promoting wound healing is characterized by comprising the following steps:
A. preparing a hydrogel pre-gel system;
B. treating the surface of the conductive metal foil by a coupling agent, and then putting the conductive metal foil into a mold;
C. and (3) adding the hydrogel pre-gel in the step (A) into the mould in the step (B), and crosslinking the hydrogel pre-gel in an ultraviolet light initiated polymerization mode for 10-100 minutes to form the metal/hydrogel composite dressing.
2. The method for preparing a metal/hydrogel composite dressing for promoting wound healing according to claim 1, wherein: in the step B, the metal is one or more of titanium, aluminum, magnesium, zinc, copper, silver, platinum or gold.
3. The method for preparing a metal/hydrogel composite dressing for promoting wound healing according to claim 1, wherein: in the step B, reactive or polar groups are introduced to the surface of the metal foil.
4. The method for preparing a metal/hydrogel composite dressing for promoting wound healing according to claim 1, wherein: in the step B, the coupling agent is one or more of a methacryloxy silane coupling agent, a vinyl silane coupling agent, an o-catechol coupling agent or a vinyl mercaptan coupling agent.
5. The method for preparing a metal/hydrogel composite dressing for promoting wound healing according to claim 1, wherein: in the step A, the hydrogel pre-gel system comprises an acrylamide monomer, a cross-linking agent containing double bonds and a water-soluble initiator.
6. The method for preparing a metal/hydrogel composite dressing for promoting wound healing according to claim 1, wherein: and C, soaking the obtained metal/hydrogel composite dressing for 3-10 days.
7. A metal/hydrogel composite dressing prepared by the method for preparing the metal/hydrogel composite dressing for promoting wound healing according to any one of claims 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810020842.1A CN107929805B (en) | 2018-01-10 | 2018-01-10 | Metal/hydrogel composite dressing for promoting wound healing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810020842.1A CN107929805B (en) | 2018-01-10 | 2018-01-10 | Metal/hydrogel composite dressing for promoting wound healing and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107929805A CN107929805A (en) | 2018-04-20 |
| CN107929805B true CN107929805B (en) | 2021-08-10 |
Family
ID=61937589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810020842.1A Active CN107929805B (en) | 2018-01-10 | 2018-01-10 | Metal/hydrogel composite dressing for promoting wound healing and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107929805B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108744068A (en) * | 2018-07-05 | 2018-11-06 | 成都碳原时代科技有限公司 | A kind of preparation and application of graphene carried medicine sustained-release electrode material |
| CN110123523A (en) * | 2019-04-16 | 2019-08-16 | 温州大学 | A kind of near-infrared stress type plasma diffusing W,Mo antiseptic dressing, the method and dressing combination that promote release |
| CN110464867B (en) * | 2019-09-25 | 2020-07-28 | 浙江大学 | Piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading traditional Chinese medicine exosomes and preparation method thereof |
| CN111662525B (en) * | 2020-07-07 | 2021-03-30 | 四川大学 | Material for flexible bioelectronic device and preparation method thereof |
| CN112451742B (en) * | 2020-11-24 | 2021-11-19 | 北京大学深圳医院 | Preparation method of degradable metal-organic matter composite bone repair material |
| CN116199910A (en) * | 2023-03-15 | 2023-06-02 | 中山大学附属口腔医院 | Conductive hydrogel dressing, preparation method and application thereof in field of diabetic wound repair |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080033506A1 (en) * | 1997-09-22 | 2008-02-07 | Argentum International, Llc | Multilayer Conductive Appliance Having Wound Healing and Analgesic Properties |
| CN101658485A (en) * | 2009-09-10 | 2010-03-03 | 东华大学 | Nanometer hybridized hydrogel with durable broad spectrum antibacterial performance and preparation method thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5489624A (en) * | 1992-12-01 | 1996-02-06 | Minnesota Mining And Manufacturing Company | Hydrophilic pressure sensitive adhesives |
| US8728445B2 (en) * | 2001-05-01 | 2014-05-20 | A.V. Topchiev Institute Of Petrochemical Synthesis, Russian Academy Of Sciences | Hydrogel Compositions |
| WO2004031253A1 (en) * | 2002-10-02 | 2004-04-15 | Coloplast A/S | A hydrogel |
| US7045559B2 (en) * | 2003-12-18 | 2006-05-16 | Kimberly-Clark Worldwide, Inc. | Electrically conductive adhesive hydrogels with solubilizer |
| JP5020141B2 (en) * | 2008-03-25 | 2012-09-05 | 積水化成品工業株式会社 | Adhesive hydrogel, composition for production thereof and use thereof |
| CN105797197B (en) * | 2016-04-25 | 2018-09-21 | 华中科技大学 | A kind of dressing for skin and preparation method thereof |
| CN106008799B (en) * | 2016-05-23 | 2018-08-17 | 西南交通大学 | A kind of preparation method of the water-setting gel electrode with strong mechanical performance and self-healing |
-
2018
- 2018-01-10 CN CN201810020842.1A patent/CN107929805B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080033506A1 (en) * | 1997-09-22 | 2008-02-07 | Argentum International, Llc | Multilayer Conductive Appliance Having Wound Healing and Analgesic Properties |
| CN101658485A (en) * | 2009-09-10 | 2010-03-03 | 东华大学 | Nanometer hybridized hydrogel with durable broad spectrum antibacterial performance and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107929805A (en) | 2018-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107929805B (en) | Metal/hydrogel composite dressing for promoting wound healing and preparation method thereof | |
| CN109550074B (en) | Conductive hydrogel for treating chronic wound and preparation method thereof | |
| CN113174074A (en) | Conductive silk fibroin film and preparation method and application thereof | |
| CN103480034B (en) | Irradiation crosslinked chitosan/gelatin/polyvinyl alcohol hydrogel dressing as well as preparation method and application thereof | |
| CN111662525B (en) | Material for flexible bioelectronic device and preparation method thereof | |
| US4893626A (en) | Electrode for electrotherapy, electrosurgery and monitoring | |
| EP1114074B1 (en) | Polymerizable material and sheet material derived therefrom | |
| CN112824439A (en) | Self-repairing antibacterial conductive hydrogel and preparation method thereof | |
| US10077324B2 (en) | Polymers, preparation and use thereof | |
| Chen et al. | Tissue-like electrophysiological electrode interface construction by multiple crosslinked polysaccharide-based hydrogel | |
| Cao et al. | Mechanoactive wound dressing using poly (N-isopropyl acrylamide) based hydrogels | |
| CN116983422A (en) | Composite system of piezoelectric nano generator/drug-loaded conductive hydrogel and preparation method and application thereof | |
| CN113730647B (en) | Flexible conductive antibacterial material and preparation method thereof | |
| CN116549723A (en) | Anti-scar dressing for shielding wound tension and preparation method thereof | |
| Gnanasambanthan et al. | Simulation and Fabrication of Micro-Electrode Arrays for Electrical Stimulation Induced Wound Healing | |
| US11517748B2 (en) | Wound treatment patch using static electricity and method for fabricating the patch | |
| Chen et al. | Effective Scald Wound Functional Recovery Patch Achieved by Molecularly Intertwined Electrical and Chemical Message in Self-Adhesive Assemblies | |
| CN113817104A (en) | Quasi-dual-network hydrogel and preparation method and application thereof | |
| JPH11290286A (en) | Electroconductive polymer gel and manufacturing method and use thereof | |
| CN101081322A (en) | Electric therapeutic appliance for improving immunity | |
| CN118000742B (en) | 3D printing hydrogel electrode for diagnosis and treatment of diabetic neuropathy | |
| CN111956861B (en) | Self-adhesive myocardial patch material and preparation method thereof | |
| KR102665816B1 (en) | Nail healing patch of hydrogel | |
| CN116920276B (en) | Wearable self-powered microneedle electric stimulation system for healing infected wound and application method thereof | |
| CN115260557A (en) | Lignin-based ultraviolet-resistant conductive hydrogel with different two-side adhesion, and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Yunbing Inventor after: Hu Xuefeng Inventor after: Zhang Jieyu Inventor after: Lu Yuhui Inventor after: Wang Yanan Inventor before: Wang Yunbing Inventor before: Hu Xuefeng Inventor before: Zhang Jieyu Inventor before: Lu Yuhui Inventor before: Wang Yanan |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |