CN107926397B - Method for enhancing prevention and treatment effect of peach fruit borers - Google Patents

Method for enhancing prevention and treatment effect of peach fruit borers Download PDF

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CN107926397B
CN107926397B CN201711225496.2A CN201711225496A CN107926397B CN 107926397 B CN107926397 B CN 107926397B CN 201711225496 A CN201711225496 A CN 201711225496A CN 107926397 B CN107926397 B CN 107926397B
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hexaflumuron
nematode
peach fruit
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nematodes
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CN107926397A (en
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武海斌
孙瑞红
宫庆涛
姜莉莉
公义
王中堂
王传增
董放
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Shandong Institute of Pomology
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract

The invention relates to a method for enhancing the control effect of peach fruit borers, which is characterized in that before the winter mature larvae of the peach fruit borers emerge and emerge from the earth and emerge every year, a hexaflumuron diluent and entomopathogenic nematodes meeting detection conditions are mixed to prepare a nematode hexaflumuron suspension, wherein the concentration of the nematodes in the nematode hexaflumuron suspension is 500-1000 IJS/mL, and the concentration of the hexaflumuron is 15-20 mg/L; when the lure is trapped to a first adult, nematode hexaflumuron suspension is applied to an orchard to kill the mature larvae of the peach fruit borer, the method can effectively solve the key technical problems that the generation period of the peach fruit borer is long in duration, the generations are overlapped, the control key period cannot be effectively grasped, and the control effect is not ideal enough, so that the offspring of the peach fruit borer can not effectively lay eggs, the egg laying amount and the hatching rate of the offspring can be maximally inhibited, and the use amount of entomopathogenic nematodes and hexaflumuron can be reduced, thereby achieving the green control target of the peach fruit borer.

Description

Method for enhancing prevention and treatment effect of peach fruit borers
Technical Field
The invention relates to a method for enhancing the control effect of peach fruit borers, and belongs to the technical field of biological control.
Background
Peach fruit borer (2)Carposina sasakiiMatsmura), also called peach fruit moth, called peach fruit moth for short, belongs to the family of lepidoptera fruit moth and mainly occurs in ChinaIn the eastern and northeast regions of China, the damage to apples, hawthorns, dates, apricots and peach trees is the most serious, and the pest is a very serious fruit-eating pest in the production of fruit trees in the northern China. Peach fruit borers occur for 1-2 generations in northern areas of China in one year, mature larvae are used as winter cocoons to overwinter in soil under trees, adults climb out of the soil to lay eggs in the next year after overwinter generation, and the larvae directly eat pulp after hatching, so that damaged fruits are filled with worm feces and can not be eaten, and the yield and the quality are directly influenced. After the larva is mature, the larva climbs out of the fruit, and then the larva enters soil to be cocooned and pupated or overwintering.
The occurrence rules of peach fruit in different areas are different. In Shandong, peaches can occur for 1-2 generations in one year, the initial period is 5, middle and last ten days or 6 months, the adult number reaches the peak period from 6, middle and last ten days, and the occurrence period lasts for nearly 3 months. In Hebei, the 3 peak periods of small peach adults occur in one year, the overwintering period begins to appear in the last 5 th month, the adult adults in the first 6 th month reach the peak period, the adult adults in the first 1 st month from the last 6 th month to the middle and last 7 months reach the peak period, and the adult adults in the second 2 nd month from the last 8 th month to the middle 9 th month are the peak period. Peach in Henan area takes place 1-3 generations in one year, and the 3 rd generation is a local generation. Apple peaches in Shaanxi region are generated for 2-3 generations in 1 year, the highest peak of adult generation amount is generated in 20 days in 7 months, and generation overlapping phenomenon exists. The generation cycle of the peach fruit borers is long, the fertility is strong, the concealment is strong, and the detection, the report and the prevention are difficult, so that the finding of the weak link of the peach fruit borers in the life history is still a key means for prevention and control.
At present, the control methods in production comprise a chemical insecticidal method, a sex attractant trapping and killing method and a biological control method, and mainly use chemical insecticides, but because the occurrence period of the peach fruit borers is long in duration, the key control period cannot be effectively grasped, the control effect is still not ideal, and the environmental pollution is easily caused. The control of the high-toxicity chemical pesticide or the increase of the pesticide application amount to the peach size not only leads to the great improvement of the drug resistance, but also seriously influences the quality of fruits. The sex attractant is mainly used for preventing and controlling the peach fruit, and the principle is that female lure cores emit female hormone in the small mating period of the peach fruit, so that male peach fruit is mistaken to be a female peach fruit moth, and the lure cores move around, and finally the male peach fruit is trapped and killed. However, the male peaches have a habit of multiple mating, and when the male sex attractant is used for trapping and killing the male sex, the male sex may complete the mating process, or the male sex which is not trapped is stimulated due to the reduction of male imagoes, so that the whole population is kept stable. Therefore, the method of adopting the sex attractant is mainly used for the detection and prediction of the insect pests, and the control effect of the sex attractant has certain limitation.
Biological control the biological control purpose is achieved by infecting the peach fruit borer with entomopathogenic nematodes, but the biological control purpose is limited by the activity and infection capacity of the entomopathogenic nematodes, and compared with chemical insecticides, the insecticidal effect is slow and unstable. Early studies can prove that the nematodes have certain control effects on the peach juveniles and pupae, but cannot effectively determine the using period of the nematodes and monitor the population base number of the adults after eclosion.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for enhancing the control effect of peach fruit borers.
Brief description of the invention:
the method aims at the problems that the damage of peach fruit is rampant, the emergence period is long in duration, generations are overlapped, the control key period cannot be effectively grasped, the control effect is not ideal enough, and the control key period cannot be accurately determined. Can be applied to control peach fruit borers harming various crops such as apples, hawthorns, dates, apricots, peach trees and the like, and achieves the purpose of green control.
The technical scheme of the invention is as follows:
a method for enhancing the control effect of peach fruit borers comprises the following steps:
suspending a peach fruit small lure core in an orchard for monitoring before the overwintering, aged and mature peach fruit borers emerge and eclose every year; diluting hexaflumuron with water to prepare a hexaflumuron diluent, and mixing the hexaflumuron diluent with entomopathogenic nematodes meeting detection conditions to prepare a nematode hexaflumuron suspension, wherein the nematode hexaflumuron suspension has a nematode concentration of 500-1000 IJS/mL and a hexaflumuron concentration of 15-20 mg/L; when the lure traps and collects the first adult, the nematode hexaflumuron suspension is applied to kill the peach fruit borer aged larvae, and normal field management is carried out after the nematode hexaflumuron suspension is applied.
Preferably, according to the invention, the entomopathogenic nematode is heterodera indicaHeterorhabditis indicaLN2。
Preferably, according to the present invention, the entomopathogenic nematodes satisfying the following conditions:
mixing the diluted hexaflumuron (50 mg/L) with the highest use concentration in the field and the nematode suspension diluted to 500 IJS/mL according to the volume ratio of 100:1, and standing for 72 hours at 24-26 ℃ for detection, wherein the survival rates of the entomopathogenic nematodes are all more than 90%.
According to the invention, the concentration of the nematodes in the nematode hexaflumuron suspension is 600-800 IJS/mL.
According to the invention, the concentration of hexaflumuron in the nematode hexaflumuron suspension is preferably 16-18 mg/L.
According to the invention, when the trap core traps the imagoes for 3 consecutive days, the application times are increased, and the application of the nematode hexaflumuron suspension is carried out for 2-3 times.
According to the invention, the soil preparation is carried out before the orchard is planted, the winter cultivation and winter application are carried out before the soil preparation, the mature larvae of the peach fruit borers in the orchard are eliminated or the garden is cleaned, and the occurrence base number of the peach fruit is reduced.
Preferably, according to the invention, the nematode hexaflumuron suspension is applied in a spray or direct watering irrigation complex.
The application times are increased, and the application of the nematode hexaflumuron suspension for the 2 nd to 3 rd times is determined according to the long duration time of the occurrence period of the peach fruit borers and the emergence peak of the mature larvae.
According to the invention, the preferable application temperature of the nematode hexaflumuron suspension is 25-30 ℃, and the water content of the soil is 10-18%.
Further preferably, the temperature of the nematode hexaflumuron suspension is 26-29 ℃ when the nematode hexaflumuron suspension is applied, and the water content of the soil is 15% -18%.
When the nematode hexaflumuron suspension is applied, the requirements on temperature and soil water content are strict, the temperature is too high or too low, and the too high or too low soil water content can cause the effect of combining heterodera indica and hexaflumuron, so that the prevention and control effect is greatly reduced.
According to the invention, the mature peach fruit borer larva is larva overwintering in soil.
Preferably, when the nematode hexaflumuron suspension is applied, the dosage of the nematode hexaflumuron suspension is 2000-3000 ten thousand per mu of nematodes.
Preferred according to the invention are heterodera indicaHeterorhabditis indicaLN2, a commercially available product, is available from ordinary commercial sources.
According to the invention, the preferred suspension height of the peach small lure is 1.2-1.8 m from the ground, and the suspension number is 3-4 per mu.
The invention has the advantages and beneficial effects
1. The invention adopts the peach small lure core to monitor the imago, and when the imago is trapped, the entomopathogenic nematode Heterodera indica is utilizedHeterorhabditis indicaLN2 is applied with hexaflumuron to treat the peach fruit borer mature larva in the overwintering state, which is respectively improved by 42.88-59.78% compared with the two, and the fruit retention effect reaches 92.19-93.46%. Greatly reduces the usage amount of entomopathogenic nematodes and hexaflumuron. The concentration of the nematode suspension in the nematode hexaflumuron suspension is 500-1000 IJS/mL, the application amount of the nematodes per mu is 2000-3000 ten thousand, and the concentration of the hexaflumuron is 15-20 mg/L. Comprehensive analysis shows that the nematode LN2 is applied to be combined with hexaflumuron before the peach fruit borer mature larva emerges and emerges, the fruit retention effect reaches 92.19-93.46%, and each kind of nematode can be greatly reducedThe use amount of the substances not only saves the cost, but also achieves the aim of safely and efficiently preventing and treating the peach fruit borers. Meanwhile, the invention can ensure that the peach fruit borer offspring does not effectively lay eggs, inhibit the egg laying amount and the hatching rate of the offspring to the maximum extent and has obvious effect. Can replace high-toxicity high-residue and other insecticidal pesticide varieties, and has not only ecological significance but also important environmental protection significance.
2. The control method provided by the invention changes the traditional control method of the peach fruit borer from on-tree control to under-tree control, can effectively solve the problems that the peach fruit borer has long duration of the emergence period, is overlapped in generation, cannot effectively grasp the control key period, and is not ideal in control effect, monitors the field emergence amount by using the small peach fruit lure core, and purposefully applies the nematode hexaflumuron suspension for 2-3 times, so that the long-term survival of the entomopathogenic nematodes in the soil is fully exerted, the advantages of parasitism and propagation are searched, and the key technical difficulty that the peach fruit borer is difficult to control is effectively solved. Not only can improve the control effect on the peach fruit borer, but also can reduce the usage amount of entomopathogenic nematodes and hexaflumuron, thereby achieving the green control target of the peach fruit borer.
Drawings
FIG. 1 is a bar graph of the effect of different concentrations of hexaflumuron on mortality for different nematodes in a field;
FIG. 2 is a bar graph of the effect of different temperatures on synergistic combinations;
FIG. 3 is a bar graph of the effect of different soil moisture contents on synergistic combinations;
Detailed Description
The technical solution of the present invention is further described with reference to the following examples, but the scope of the present invention is not limited thereto.
The small peach lure in the embodiment is a commercial product.
Laboratory test determination
1. Nematode and hexaflumuron compatibility assay
To test 5% hexaflumuron emulsifiable concentrate, Zhejiang Shiyuan Taurus pesticide, Ltd. Steinernema cuneaSteinernema carpocapsaeNC116 (NC 116), Plutella xylostellaSteiner wormSteinernema carpocapsaeAll (all), wirewormSteinernema longicaudumX-7 (X-7), Spodoptera frugiperda (Spodoptera frugiperda)Steinernema feltiaeSF-SN (SF), Heterorhabditis bacteriovoraHeterorhabditis bacteriophoraH06 (H06), Heterodera indicaHeterorhabditis indicaLN2 (LN 2). The entomopathogenic nematodes are all common entomopathogenic nematodes in the field and are available from green agricultural biotechnology limited.
The dilution of hexaflumuron (50 mg/L) with the highest concentration used in the field and the nematode suspension diluted to 500 IJS/mL are mixed according to the volume ratio of 100:1, each treatment is repeated for 3 times, and the clear water nematode suspension with the nematode concentration of 500 IJS/mL is used as a control. The mixture was left at 25. + -. 1 ℃ for 24 hours, and the survival rate of nematodes was calculated by randomly examining the survival rate of nematodes in 1 mL of the mixture per treatment under a microscope. If the lethal or sublethal effect of chlorbenzuron on nematodes does not exceed 10%, the effect of the insecticide on nematode infestation is continuously determined. Determining the reaction standard of entomopathogenic nematodes to the medicament by referring to Weihongyi: (1) death: the body is stiff or curly, inactive and has no response to acupuncture; (2) sublethal: body curl, which is either unresponsive to a needle stick or very sluggish in response to a spasmodic or tic activity due to the paralytic effects of the agent; (3) is not affected by the medicament: as with control nematodes, the body is moving and is stretched or immobile, and the resting nematodes respond rapidly upon contact with the probe.
The results of the tests (fig. 1) show that: the highest concentrations of hexaflumuron all have varying degrees of mortality from nematodes, but the upper limit of mortality has not been exceeded by 10%. The difference between the nematode mortality of the mixed solution of the insecticide and the nematodes and the mortality of the nematodes compared with that of the clear water is not obvious. Indicating that hexaflumuron had essentially no effect on nematode survival.
2. Determination of virulence of different nematode strains on peach fruit borer mature larva
And (3) determining the pathogenicity of different strains of nematodes on the small and old peach larvae by adopting a culture dish sawdust method. Each test nematode suspension was diluted to 5 concentrations in an equal ratio with distilled water. Placing two pieces of medium-speed filter paper in a culture dish with the diameter of 15 cm, filling the sterilized sawdust, respectively adding each nematode treating fluid to make the water content of the nematode treating fluid 20%, placing 20 mature larvae, repeating the treatment for 4 times, and treating with distilled water as a blank control. In a light incubator [ T =24 ± 1 ℃, L: D =16:8 h, RH = (60 ± 10)% ]. And processing for 3d, checking the death condition of the aged larvae, calculating and correcting the death rate, and solving a toxicity regression equation.
Figure DEST_PATH_IMAGE001
The test results (table 1) show that: entomopathogenic nematodes of different strains have varying degrees of pathogenicity to mature larvae of Buckoo peach with the more virulent strains H06, LN2 and X-7, of which the most virulent strain is the LN2 strain, the lethal median LD is5013.90 pieces/worm, H06 line also had higher virulence, its LD5023.10 shoots/worm, while the SF-SN line is least pathogenic and its LD50111.60 pieces/insect.
3. Determination of effect of mixture of hexaflumuron and nematodes on mature larvae of peach fruit borers
Setting up monohexaflumuron (15 mg/L), monohexaflumuron (20 mg/L) and mono LN2Nematode (10 nematodes/worm), Single LN2Nematodes (15 per worm), hexaflumuron (15 mg/L) + LN2 (10 per worm), hexaflumuron (20 mg/L) + LN2 (15 per worm) and a bare water control. The test method and conditions were the same as above. The death, pupation and eclosion conditions of the two kinds of old larvae of the cardiophagemid are checked every day. And moving the eclosion peach fruit borers into an eclosion device with egg-laying paper attached to the inner wall, supplementing nutrition with 5% honey water, observing and recording the egg-laying amount and egg incubation condition day by day. And calculating the mortality, pupation rate, eclosion rate, single-female egg laying amount and egg hatching rate.
The death effect is calculated by Zhang Zhongrun method, and is calculated by Chi2And (4) judging the combined action type of the nematodes and the pesticides on the peach fruit borers by an independence test. When x2When the concentration is less than 3.84, the mixture of the two insecticidal factors shows additive effect when the concentration is higher than2When the insecticidal composition is more than or equal to 3.84, the synergistic effect of the two insecticidal factors is shown.
Figure DEST_PATH_IMAGE002
The test results (table 2) show that: the use of a single dose of hexaflumuron or LN2 nematode corrected mortality significantly lower than the combination of the two. The effect of the mixed treatment of hexaflumuron (20 mg/L) + LN2 (15 per insect), hexaflumuron (15 mg/L) + LN2 (10 per insect) shows synergistic effect, the emergence rate is 0, which is obviously lower than that of clear water control, the offspring does not effectively lay eggs, the prevention and treatment effect on the aged larvae can be effectively improved, the egg laying amount and the hatching rate of the offspring can be inhibited to the maximum degree, and the effect is obvious.
4. Influence of different temperatures on the effect of the synergistic combination
Setting monohexaflumuron (15 mg/L) and single LN2Nematodes (10 per worm), hexaflumuron (15 mg/L) + LN2 (10 per worm) and a clear water control. The test method and conditions were the same as above. Each treatment was repeated 4 times. The water content was 10%, and each treatment was treated in a light incubator at 10, 15, 20, 25, and 30 ℃ for 3 days, and the death of the aged larvae was examined and the corrected mortality was calculated.
The results of the test (fig. 2) show that: along with the rise of the temperature, the effect of each treatment on the peach fruit borer aged larvae is obviously improved. The effect of the hexaflumuron + LN2 combination was higher than that of monohexaflumuron and monoworms at different temperatures. Wherein the action effect of the hexaflumuron and LN2 combination is 10, 15 and 20 ℃ at 25 and 30 ℃, and the synergistic effect is shown, namely chi of the hexaflumuron and LN2 combination237.59 and 22.99 respectively, and the difference of the control effect is not significant at 25 ℃ and 30 ℃. Therefore, the optimum temperature range of the hexaflumuron and LN2 combination for the insecticidal action is 25-30 ℃.
5. Influence of different soil water contents on synergistic effect
Setting monohexaflumuron (15 mg/L) and single LN2Nematodes (10 per worm), hexaflumuron (15 mg/L) + LN2 (10 per worm) and a clear water control. The test method and conditions were the same as above. Each treatment was repeated 4 times. The soil water content is respectively set to be 2%, 6%, 10%, 14% and 18%, and the soil is placed in an incubator at the temperature of 25 ℃ for treatment 3And d, checking the death condition of the aged larvae, and calculating the corrected mortality.
The results of the test (fig. 3) show that: the same treatment has different effects on the peach fruit borer mature larva under different humidities. The effect of the hexaflumuron + LN2 combination is higher than that of the monohexaflumuron and the monozoa under different water contents, wherein the effect of the hexaflumuron + LN2 combination is significant by 2% and 6% at 10%, 14% and 18% water contents, and is shown as a synergistic effect, namely chi221.76, 21.64 and 20.74 respectively, and the difference of the control effect is not significant at 25 ℃ and 30 ℃. Therefore, the optimum soil water content range of the hexaflumuron and LN2 combination for exerting the insecticidal effect is 10% -18%.
Verification of field test
The test selects an apple orchard to carry out field test verification, the test is carried out in an apple plantation in Langa mountain apple planting base in Laizhou, Shandong province, the soil is sandy loam which is loose and has good air permeability, the variety of the apple is red Fuji, the plant spacing is 3 m × 4 m, the area of each plot is 250 m, and the plot is in the form of a small plot, wherein the small peach borer is called peach fruit, mainly occurs in regions which are eastern parts and northern parts of China, has the most serious harm to apples, hawthorns, dates, apricots and peach trees, and is a fruit-eating pest which has the most serious harm to the fruits in the fruit trees in northern parts of China2Isolation regions are arranged between different treatments, repeated for 4 times and randomly arranged. The sex attractant is adopted to measure and report the peach fruit borer imagoes, the nematode hexaflumuron suspension is poured into the field at the beginning of 4 months to 5 months every year, the nematode concentration is 500-1000 IJS/mL in the suspension, and the dosage of the nematodes is 10-15 per peach fruit. The concentration of hexaflumuron is 15-20 mg/L. The suspension height of the lure core is 1.5m, the spacing is 15m, the insect fruit rate is investigated in the harvest period, and the fruit retention rate is calculated. Entomopathogenic nematodes screened as described aboveHeterorhabditis indicaLN2 was tested. The concentration of the nematode in the suspension is 500-1000 IJS/mL. The application amount of the nematodes per mu is 2000-3000 thousands, and the number of the lure hanging is 3 per mu.
Example 1
A method for enhancing the control effect of peach fruit borers comprises the following steps:
(1) and selecting loose and ventilated soil for soil preparation, planting apples, wherein the variety is Fuji, and the row spacing is 3 m multiplied by 4 m.
(2) And after the adult insects enter the middle ten (4) months, suspending a small peach attracting core for monitoring, and applying the nematode hexaflumuron suspension to the apple orchard when the first adult insects are attracted. Diluting hexaflumuron with water to obtain diluted hexaflumuron solution, and mixing it with entomopathogenic nematodes meeting the detection conditionHeterorhabditis indicaLN2 to obtain a suspension of nematode hexaflumuron, wherein the concentration of the nematode hexaflumuron in the suspension of nematode hexaflumuron is 800 IJS/mL, and the concentration of hexaflumuron is 15 mg/L; when the nematode hexaflumuron suspension is applied, the dosage of the nematode hexaflumuron suspension meets 2000 ten thousand nematodes per mu, the peach fruit borer mature larvae are killed, and normal field management is carried out after the nematode hexaflumuron suspension is applied.
Example 2
The method for enhancing the control effect of the peach fruit borers is the same as that in example 1, except that the dosage of the nematode hexaflumuron suspension is 2500 ten thousand nematodes per mu, and the concentration of the hexaflumuron in the nematode hexaflumuron suspension is 18 mg/L.
Example 3
The method for enhancing the control effect of the peach fruit borers is the same as that in example 1, except that the dosage of the nematode hexaflumuron suspension is 3000 ten thousand nematodes per mu, and the concentration of the hexaflumuron in the nematode hexaflumuron suspension is 18 mg/L.
Comparative example 1
The method for enhancing the control effect of peach fruit borer as described in example 1, except that the entomopathogenic nematode was entomopathogenic nematode H06, which was used in an amount of 3000 ten thousand nematodes per mu, and the concentration of hexaflumuron in the nematode hexaflumuron suspension was 18 mg/L. For comparison with the effects of the present invention.
Comparative example 2
The method for enhancing the control effect of peach fruit borer as described in example 1, except that entomopathogenic nematodes were entomopathogenic nematodes X-7, which were used in an amount of 3000 ten thousand nematodes per mu, and the concentration of hexaflumuron in the nematode hexaflumuron suspension was 18 mg/L, for comparison with the effect of the present invention
Test examples
The field test is provided with 11 treatments respectivelyGrapholitha was used alone for sex-attractant core, hexaflumuron alone (hexaflumuron group 100 mg/L), nematodes alone (LN 2 nematodes, H06 nematodes, X-7 nematodes), hexaflumuron application and nematode application (example 1, example 2, example 3, comparative 1 and comparative 2) and blank control (control). Wherein the steps of applying hexaflumuron and applying nematodes are 250 m per treated area of the cell using the methods described in example 1, example 2, example 3, comparative example 1 and comparative example 22Repeat 4 times, random arrangement. The specific conditions and procedures are shown in Table 3.
Figure DEST_PATH_IMAGE003
As can be seen from Table 3, the fruit retention effects of examples 1, 2, 3, 1 and 2 on apples are all significantly better than the effects of the sex attractant alone, the nematode alone and the hexaflumuron alone; the fruit retention effect of the samples of example 1, example 2 and example 3 using LN2 is higher than that of comparative example 1 using nematode H06 and comparative example 2 using nematode X-7. This is mainly due to the different pathogenicity of different nematode strains on the pests. The good effect is probably shown because on one hand, the defense capacity of pests to the nematodes can be reduced, and the nematode invasion is facilitated, and on the other hand, the medicament stimulates the sensory nerves of the nematodes, so that the nematodes are excited and the movement is increased, and the searching and attacking capacity of the nematodes to the pests is enhanced. The fruit retention effect of the fruits of the embodiments 1, 2 and 3 is respectively improved by 42.88 to 59.78 percent compared with that of the fruits of the two single-use fruits. Comprehensive analysis shows that the nematode LN2 is applied to be combined with hexaflumuron before the mature larvae of the peach fruit borers emerge and emerge, and the fruit retention effect reaches 92.19-93.46%.

Claims (6)

1. A method for enhancing the control effect of peach fruit borers comprises the following steps:
soil preparation is carried out before orchard planting, winter ploughing and winter application are carried out before soil preparation, mature larvae of the peach fruit borers in the orchard are eliminated or the garden is cleaned, and the occurrence base number of the peach fruit borers is reduced; suspending a peach fruit small lure core in an orchard for monitoring before the overwintering, aged and mature peach fruit borers emerge and eclose every year; diluting hexaflumuron with water to prepare a hexaflumuron diluent, and mixing the hexaflumuron diluent with entomopathogenic nematodes meeting detection conditions to prepare a nematode hexaflumuron suspension, wherein the nematode hexaflumuron suspension has a nematode concentration of 500-1000 IJS/mL and a hexaflumuron concentration of 15-20 mg/L; when the first adult insects are trapped and collected by the trapping cores, the nematode hexaflumuron suspension is applied to kill the old peach fruit borers in an orchard in a mode of matching spraying and direct watering irrigation, and normal field management is performed after the application;
the entomopathogenic nematode is heterodera indicaHeterorhabditis indicaLN 2; the application temperature of the nematode hexaflumuron suspension is 25-30 ℃, and the water content of the soil is 10-18%.
2. The method for enhancing the control effect of peach fruit borer according to claim 1, wherein the entomopathogenic nematodes satisfying the following conditions:
mixing the hexaflumuron diluent with the highest use concentration of 50 mg/L in the field and the nematode suspension diluted to 500 IJS/mL according to the volume ratio of 100:1, and standing for 72 hours at 24-26 ℃ for detection, wherein the survival rates of the entomopathogenic nematodes are above 90%.
3. The method for enhancing the control effect of peach fruit borer according to claim 1, wherein when the adult insects are induced to the lure for 3 consecutive days, the number of application is increased and the application of the nematode hexaflumuron suspension is performed 2 to 3 times.
4. The method for enhancing the control effect of Grapholitha persiciflorus as claimed in claim 1, wherein the temperature of the nematode hexaflumuron suspension is 26-29 ℃ and the water content of the soil is 15-18%.
5. The method of enhancing the control of peach fruit borer according to claim 1, wherein the amount of the nematode hexaflumuron suspension applied is such that 2000 to 3000 million nematodes per mu.
6. The method for enhancing the control effect of peach fruit borers according to claim 1, wherein the hanging height of the peach fruit borers is 1.2 to 1.8 m from the ground, and the hanging number is 3 to 4/mu.
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