CN107916105B - Red fluorescent carbon quantum dot for detecting intracellular pH and preparation method thereof - Google Patents
Red fluorescent carbon quantum dot for detecting intracellular pH and preparation method thereof Download PDFInfo
- Publication number
- CN107916105B CN107916105B CN201711142529.7A CN201711142529A CN107916105B CN 107916105 B CN107916105 B CN 107916105B CN 201711142529 A CN201711142529 A CN 201711142529A CN 107916105 B CN107916105 B CN 107916105B
- Authority
- CN
- China
- Prior art keywords
- carbon quantum
- quantum dot
- phenylenediamine
- quantum dots
- putting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Optics & Photonics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Manufacturing & Machinery (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a red fluorescent carbon quantum dot for detecting pH in cells and a preparation method and application thereof. The preparation method comprises the following steps: (1) weighing 0.1-0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.075-0.15mol of ethylenediamine to obtain light yellow liquid; (2) placing the solution in a microwave oven, reacting for 5-25 min under 20-100% of power, cooling to room temperature after the reaction is stopped, and adding 5mol/L HCl until gas escapes; (3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a container filled with water, dialyzing for two days to obtain a pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots. The carbon quantum dot prepared by the invention has the characteristics of long emission, high quantum yield, good biocompatibility and the like; the carbon quantum dots have sensitive response to pH, and can penetrate through a nuclear membrane to enter a cell nucleus so as to enter the whole cell, thereby achieving the effect of detecting the pH of the whole cell.
Description
Technical Field
The invention relates to a nano material, in particular to a red fluorescent carbon quantum dot for detecting the pH value in a whole cell and a preparation method and application thereof.
Background
The pH value of cells is an important parameter in metabolism and cells, and plays a key role in regulating physiological and pathological processes of many cells. Abnormal pH can inhibit the function disorder of cells, tissues and organs in vivo, inhibit the activity of enzymes and proteins, reduce the immunity of human bodies, and finally cause diseases such as inflammation, cancer, Alzheimer's disease and the like. In addition, changes in cell pH are closely associated with apoptosis. Cancer cells, in the early stage of apoptosis, are commonly associated with intracellular acidification, which has become an important early feature of apoptosis. Therefore, the sensitive and accurate real-time in-situ monitoring of the pH of the cells is helpful for understanding the physiological and pathological processes of the cells from the molecular level.
The fluorescence spectroscopy has the advantages of high sensitivity, good selectivity, quick response, high signal-to-noise ratio, simple and convenient operation and the like, and the pH fluorescent probe can monitor the dynamic distribution and the regional change of the pH in the cell in situ in real time by combining the fluorescence microscopic imaging technology and shows the unique property of high time and space resolution.
As a novel carbon nano material, the carbon quantum dot has excellent performances of good water solubility, biocompatibility, low toxicity, light stability, easy functionalization, photobleaching resistance and the like, and has good application prospects in various fields of biological imaging, biological sensing, drug delivery and the like. In recent years, synthesized carbon quantum dots generally emit blue and green fluorescence, which limits their application in the biological field. In addition, in the aspect of cell imaging, most of the current fluorescent nanoprobes are only limited in a cell matrix in the aspect of cell imaging and cannot penetrate through a nuclear membrane to enter a cell nucleus. Therefore, the carbon quantum dots which realize efficient red light emission and can enter cell nuclei through nuclear membranes have very important significance. In order to solve the problems, the invention provides a method for synthesizing carbon quantum dots by using p-phenylenediamine and ethylenediamine as raw materials through a microwave method, and the method is finally used for detecting the pH value in biological cells.
Disclosure of Invention
The invention aims to provide a red carbon quantum dot which can penetrate through a nuclear membrane to enter a cell nucleus and is used for detecting the pH of the whole cell, and a preparation method and application thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
preparation method of red fluorescent carbon quantum dots for detecting intracellular pH
(1) Weighing 0.1-0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.075-0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 5-25 min under the power of 20-100%, cooling to room temperature after the reaction is stopped, and adding 5mol/LHCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a container filled with water, dialyzing for two days to obtain a pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots.
The obtained carbon quantum dots are dissolved in a PBS buffer solution and added into a culture solution of the A549 cells, incubation is carried out for 6h, and then the imaging state of the A549 cells is observed through a laser confocal microscope. When the carbon quantum dot is at pH 5.8, the cells show bright red fluorescence; at pH 7.4, the carbon quantum dots showed very weak fluorescence by the cells. The carbon quantum dot can be used as a detection reagent for detecting the pH of the whole cell including the cell nucleus.
Compared with the prior art, the invention has the following advantages:
the carbon quantum dot prepared by the invention has the characteristics of long emission, high quantum yield, good biocompatibility and the like;
the carbon quantum dots prepared by the method have sensitive response to pH, and can penetrate through a nuclear membrane to enter a cell nucleus so as to enter the whole cell, thereby achieving the effect of detecting the pH of the whole cell.
Drawings
FIG. 1 is a transmission electron micrograph of a fluorescent carbon quantum dot prepared according to example 1 of the present invention;
FIG. 2 shows the UV absorption spectrum and fluorescence excitation-emission spectrum of the fluorescent carbon quantum dot prepared in example 1 of the present invention;
FIG. 3 is a graph (1.4-7.4) showing the change of fluorescence intensity at 592nm with pH value of the fluorescent carbon quantum dot prepared in example 1 of the present invention;
fig. 4 is a confocal display diagram of laser light when the fluorescent carbon quantum dots prepared in example 1 of the present invention enter the whole cell under the conditions of pH 5.8 and pH 7.4, respectively.
Detailed Description
Example 1
In this embodiment, a preparation method of a red fluorescent carbon quantum dot for detecting intracellular pH includes the following steps:
(1) weighing 0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 20min under 20% power, cooling to room temperature after the reaction is stopped, and adding 5mol/LHCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a 1000ml beaker, dialyzing for two days to obtain pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 14%.
The obtained carbon quantum dots were dissolved in PBS buffer solutions with pH 5.8 and pH 7.4, respectively, to prepare solutions of 50 μ g/ml, which were added to the culture solutions of a549 cells, respectively, incubated for 6 hours, and then the imaging state of a549 cells was observed by a confocal laser microscope.
The synthesized carbon quantum dots are structurally characterized, as shown in fig. 1. The carbon quantum dots are uniform spherical in shape and have a particle size of about 4.6 nm.
The spectral properties of the synthesized carbon quantum dots were studied, as shown in fig. 2. The optimal excitation wavelength and the optimal emission wavelength of the carbon quantum dot are 535nm and 592nm, respectively.
The synthesized carbon quantum dots show fluorescence changes at different pH values, as shown in FIG. 3. The carbon quantum dots exhibit sigmoidal curves at pH 2.0-9.0 with pKa 5.8 and linear range 4.5-7.0.
The synthesized carbon quantum dots were observed by confocal laser experiments after entering cells, as shown in fig. 4. At pH 5.8, the cells exhibited bright red fluorescence, and it was clearly observed from the image of the cells that the entire nucleus exhibited bright red fluorescence, indicating successful entry of the synthesized carbon quantum dot into the nucleus. At pH 7.4, the carbon quantum dots showed very weak fluorescence by the cells. Indicating that the carbon quantum dot can be successfully used for detecting the pH of the whole cell including the cell nucleus.
Example 2
(1) Weighing 0.1mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 20min under 40% power, cooling to room temperature after reaction is stopped, and adding 5mol/LHCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a 1000ml beaker, dialyzing for two days to obtain pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 12%.
Example 3
(1) Weighing 0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.075mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 15min under 60% power, cooling to room temperature after the reaction is stopped, and adding 5mol/LHCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a 1000ml beaker, dialyzing for two days to obtain pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 10%.
Example 4
(1) Weighing 0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 10min under 80% power, cooling to room temperature after the reaction is stopped, and adding 5mol/LHCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a 1000ml beaker, dialyzing for two days to obtain pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 11%.
Example 5
(1) Weighing 0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 5min under 100% power, cooling to room temperature after reaction is stopped, and adding 5mol/LHCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a 1000ml beaker, dialyzing for two days to obtain pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots. The quantum yield of the carbon quantum dots was measured to be 8%.
Comparative example
(1) Weighing 0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 5min under 20% power, and cooling to room temperature after reaction is stopped;
(3) putting the obtained solution into a 500-Da dialysis bag, putting the dialysis bag into a 1000ml beaker, dialyzing for two days to obtain a pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots, wherein the quantum yield is 3%.
Compared with the carbon quantum dot obtained in example 1, the quantum yield and the yield of the obtained carbon quantum dot are obviously reduced, and the properties are unstable.
Claims (3)
1. A preparation method of a red fluorescent carbon quantum dot for detecting pH in cells is characterized by comprising the following steps:
(1) weighing 0.1-0.2mmol of p-phenylenediamine and dissolving the p-phenylenediamine in 0.075-0.15mol of ethylenediamine to obtain light yellow liquid;
(2) placing the solution in a microwave oven, reacting for 5-25 min under 20-100% of power, cooling to room temperature after the reaction is stopped, and adding 5mol/L HCl until gas escapes;
(3) putting the obtained solution into a 500-1000Da dialysis bag, putting the dialysis bag into a container filled with water, dialyzing for two days to obtain a pure carbon quantum dot aqueous solution, and freeze-drying the pure carbon quantum dot aqueous solution to obtain light pink flocculent carbon quantum dots.
2. The method for preparing red fluorescent carbon quantum dots according to claim 1, wherein the amount of p-phenylenediamine in the step (1) is 0.2mmol, and the amount of ethylenediamine in the step (1) is 0.15 mol.
3. The method for preparing red fluorescent carbon quantum dots according to claim 1, wherein the microwave oven power in the step (2) is 20%, and the reaction time is 20 min.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711142529.7A CN107916105B (en) | 2017-11-17 | 2017-11-17 | Red fluorescent carbon quantum dot for detecting intracellular pH and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711142529.7A CN107916105B (en) | 2017-11-17 | 2017-11-17 | Red fluorescent carbon quantum dot for detecting intracellular pH and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107916105A CN107916105A (en) | 2018-04-17 |
CN107916105B true CN107916105B (en) | 2020-11-10 |
Family
ID=61896586
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711142529.7A Active CN107916105B (en) | 2017-11-17 | 2017-11-17 | Red fluorescent carbon quantum dot for detecting intracellular pH and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107916105B (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109111917B (en) * | 2018-07-23 | 2021-06-25 | 中国科学院合肥物质科学研究院 | Crosslinked carbon quantum dot nanosphere fluorescent probe material and preparation method and application thereof |
CN109054826B (en) * | 2018-09-12 | 2021-03-30 | 山西大学 | Red fluorescent carbon quantum dot and preparation method and application thereof |
CN109897634B (en) * | 2019-04-24 | 2020-10-09 | 江南大学 | pH-sensitive long-wavelength fluorescent carbon dot and biological application thereof |
CN110055063A (en) * | 2019-05-09 | 2019-07-26 | 山西大学 | A kind of B, N, fluorescent orange carbon dots of S codope and its preparation method and application |
CN110208547B (en) * | 2019-06-27 | 2022-07-08 | 北京柏海达科技有限公司 | Pepsinogen immunochromatography detection kit based on carbon quantum dots and preparation method thereof |
CN111662711A (en) * | 2020-06-18 | 2020-09-15 | 长春工业大学 | Preparation method and application of red fluorescent carbonized polymer dots |
CN113150776B (en) * | 2021-01-22 | 2022-09-02 | 季华实验室 | Red fluorescent carbon quantum dot, preparation method thereof and fluorescent probe |
CN114735674B (en) * | 2022-05-09 | 2023-12-08 | 辽宁大学 | Carbon quantum dot capable of releasing hydrogen sulfide gas and preparation method thereof |
CN114907845A (en) * | 2022-06-15 | 2022-08-16 | 辽宁大学 | Three-primary-color fluorescent carbon quantum dot and preparation method and application thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105036111A (en) * | 2015-07-17 | 2015-11-11 | 清华大学 | Atmospheric micro plasma assisted preparation method and application of acid resistant carbon quantum dot |
CN106323931A (en) * | 2016-09-17 | 2017-01-11 | 湖南科技大学 | Method for quickly synthesizing carbon dots in microwave manner by using yeast bacteria as a main carbon source and using carbon dots to detect pH of solution |
CN106675558A (en) * | 2017-01-16 | 2017-05-17 | 华南农业大学 | Carbon quantum dot/Eu<3+>/mesoporous alumina composite luminescent material and preparation and application thereof |
CN106867525A (en) * | 2017-01-16 | 2017-06-20 | 华南农业大学 | Fluorescent carbon quantum dot/meso-porous alumina composite luminescent material and its preparation method and the application in terms of oxygen sensor |
CN107640759A (en) * | 2017-10-20 | 2018-01-30 | 山西大学 | PH responsive type feux rouges carbon quantum dots in weak acid pattern and preparation method thereof |
-
2017
- 2017-11-17 CN CN201711142529.7A patent/CN107916105B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105036111A (en) * | 2015-07-17 | 2015-11-11 | 清华大学 | Atmospheric micro plasma assisted preparation method and application of acid resistant carbon quantum dot |
CN106323931A (en) * | 2016-09-17 | 2017-01-11 | 湖南科技大学 | Method for quickly synthesizing carbon dots in microwave manner by using yeast bacteria as a main carbon source and using carbon dots to detect pH of solution |
CN106675558A (en) * | 2017-01-16 | 2017-05-17 | 华南农业大学 | Carbon quantum dot/Eu<3+>/mesoporous alumina composite luminescent material and preparation and application thereof |
CN106867525A (en) * | 2017-01-16 | 2017-06-20 | 华南农业大学 | Fluorescent carbon quantum dot/meso-porous alumina composite luminescent material and its preparation method and the application in terms of oxygen sensor |
CN107640759A (en) * | 2017-10-20 | 2018-01-30 | 山西大学 | PH responsive type feux rouges carbon quantum dots in weak acid pattern and preparation method thereof |
Non-Patent Citations (2)
Title |
---|
Microplasma-assisted rapid synthesis of luminescent nitrogen-doped carbon dots and their application in pH sensing and uranium detection;Zhe Wang et al.;《Nanoscale》;20151111;第7卷;20743-20748 * |
氮掺杂碳纳米粒子荧光共振能量转移测定核黄素;张士超等;《化学试剂》;20170915;982-986 * |
Also Published As
Publication number | Publication date |
---|---|
CN107916105A (en) | 2018-04-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107916105B (en) | Red fluorescent carbon quantum dot for detecting intracellular pH and preparation method thereof | |
Park et al. | Nonblinking and nonbleaching upconverting nanoparticles as an optical imaging nanoprobe and T1 magnetic resonance imaging contrast agent | |
Radunz et al. | Simple self-referenced luminescent pH sensors based on upconversion nanocrystals and pH-sensitive fluorescent BODIPY dyes | |
Wang et al. | A Hydrogen‐Bonded‐Supramolecular‐Polymer‐Based Nanoprobe for Ratiometric Oxygen Sensing in Living Cells | |
CN109490269B (en) | Preparation method of dual-emission colorimetric fluorescent nano-microsphere and application of dual-emission colorimetric fluorescent nano-microsphere in bacterial detection | |
CN109897634B (en) | pH-sensitive long-wavelength fluorescent carbon dot and biological application thereof | |
CN110982513B (en) | Preparation method of fluorescent carbon dots and application of fluorescent carbon dots in cell imaging | |
Roming et al. | ZrO (HPO4) 1− x (FMN) x: Quick and easy synthesis of a nanoscale luminescent biomarker | |
Shan et al. | Novel N-doped carbon dots prepared via citric acid and benzoylurea by green synthesis for high selectivity Fe (III) sensing and imaging in living cells | |
CN110511751B (en) | Tunable dual-emission fluorescent carbon dot, and preparation method and application thereof | |
CN106085416A (en) | A kind of aggregation-induced emission nanoparticle and its preparation method and application | |
CN111876149A (en) | Preparation method and application of ratio type fluorescent carbon dots for detecting sertraline and glutathione | |
Terrones et al. | A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: performance in vitro and in live cells | |
CN109486481B (en) | Is used for detecting Ag+GSH (glutathione) ratio type fluorescent carbon dot and preparation method thereof | |
Xu et al. | One-step synthesis of europium complexes containing polyamino acids through ring-opening polymerization and their potential for biological imaging applications | |
Yao et al. | Persistent luminescence nanoparticles/hierarchical porous ZIF-8 nanohybrids for autoluminescence-free detection of dopamine | |
Wang et al. | Fluorescein isothiocyanate-doped conjugated polymer nanoparticles for two-photon ratiometric fluorescent imaging of intracellular pH fluctuations | |
CN113604213B (en) | Nano fluorescent probe and preparation method and application thereof | |
Yu et al. | Highly luminescent gold nanocluster assemblies for bioimaging in living organisms | |
CN110208236A (en) | Ratio-type fluorescent pH nano-probe based on apoferritin, preparation method and applications | |
Pan et al. | Viscosity-sensitive thiolated gold nanoclusters with diffusion-controlled emission for intracellular viscosity imaging | |
CN110117492A (en) | A kind of fluorescent carbon point and the preparation method and application thereof | |
Xu et al. | Lipid droplet formation and dynamics: tracking by time-resolved fluorescence imaging | |
CN111573654A (en) | Green fluorescent carbon quantum dot for detecting pH value in acidic environment and preparation method thereof | |
CN114806556B (en) | Red fluorescent carbon quantum for pH ratio determination and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |