CN107904184A - Acinetobacter calcoaceticus for oil degradation and its preparation method and application - Google Patents
Acinetobacter calcoaceticus for oil degradation and its preparation method and application Download PDFInfo
- Publication number
- CN107904184A CN107904184A CN201710328505.4A CN201710328505A CN107904184A CN 107904184 A CN107904184 A CN 107904184A CN 201710328505 A CN201710328505 A CN 201710328505A CN 107904184 A CN107904184 A CN 107904184A
- Authority
- CN
- China
- Prior art keywords
- oil
- acinetobacter calcoaceticus
- degradation
- culture
- oil degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A kind of Acinetobacter calcoaceticus for oil degradation, this is used for the entitled of the Acinetobacter calcoaceticus of oil degradation：Acinetobacter calcoaceticus JH250 8, latin name：Acinetobacter baumannii JH250 8, deposit number are:CGMCC No.8902；Preservation date：On 03 07th, 2014；Preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC.Acinetobacter calcoaceticus of the present invention is under conditions of oil content is 1%, its oil degradation efficiency (gravimetric method) is up to more than 90% in 3 days, under the conditions of 5% petroleum concentration, its oil degradation efficiency (gravimetric method) is up to 80% or so in 3 days.There are higher oil degradation efficiency and the tolerance of high concentration oil.
Description
Technical field
The present invention relates to microbiological petroleum degraded field, more particularly to a kind of Acinetobacter calcoaceticus for oil degradation
And its preparation method and application.
Background technology
Oil supplies raw material as the basic energy resource of modern industry, has important work in the development of whole human society
With, but with more and more oil exploitations and application, caused by oil environmental pollution be also increasingly subject to people's
Pay attention to, there is presently no preferable processing mode.
Microbial degradation is as a kind of non-secondary pollution, and effective petroleum pollution mode of low cost is more next
More it is valued by people, the alkane degradation enzyme of certain micro-organisms expression can decompose long chain alkane class chemical combination efficiently, in specific manner
Thing, is allowed to change into certain water miscible carboxylic acid and utilized by organism.Although there is this kind of enzyme gene in many bacterial strains
In the presence of, but the bacterial strain for the alkane that can effectively degrade is actually rare.The oil degradation bacterial strain degradation efficiency reported is more below 60%,
Particularly at home, the microorganism of efficient degradation ability has to be developed.
Based on this, there is provided a kind of efficient oil degradation microorganism and correlation method just seem particularly necessary.
The content of the invention
To solve the above-mentioned problems of the prior art, it is an object of the invention to provide a kind of vinegar for oil degradation
Sour calcium acinetobacter calcoaceticus and its preparation method and application.Acinetobacter calcoaceticus of the present invention is in the bar that oil content is 1%
Under part, its oil degradation efficiency (gravimetric method) is up to more than 90% in 3 days, and under the conditions of 5% petroleum concentration, its oil drops in 3 days
Efficiency (gravimetric method) is solved up to 80% or so.There are higher oil degradation efficiency and the tolerance of high concentration oil.
To reach above-mentioned purpose, the technical scheme is that：
A kind of Acinetobacter calcoaceticus for oil degradation, it is entitled：Acinetobacter calcoaceticus JH250-8, latin name：
Acinetobacterbaumannii JH250-8, deposit number are:CGMCCNo.8902；Preservation date：03 month 2014 07
Day；Preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Depositary institution：Chinese microorganism strain preservation conservator
Can common micro-organisms center (CGMCC).
Further, its 16S rDNA sequence of the Acinetobacter calcoaceticus for oil degradation such as SEQ ID No.1
It is shown.
Further, the Acinetobacter calcoaceticus for oil degradation, decomposable asymmetric choice net alkane derivative, including 12
Alkane, tridecane, the tetradecane, pentadecane, hexadecane, heptadecane, octadecane, nonadecane, eicosane, heneicosane, docosane,
Tricosane, lignocerane, pentacosane, hexacosane, heptacosane, octacosane, nonacosane.
Further, the Acinetobacter calcoaceticus for oil degradation is in the culture medium of crude oil is contained only, in 3 days
Oil content can be decomposed for more than 90% alkane in 0.6% crude oil culture medium.
Further, the Acinetobacter calcoaceticus for oil degradation, in normal metabolic processes can synthetic glycerine,
Palmitic acid, stearic acid are as metabolite, and three kinds of metabolites can be secreted into extracellularly；Wherein glycerine, palmitic acid are in born of the same parents
Outer concentration is higher than intracellular, and stearic acid intracellular extracellular concentration is close；Intracellular palmitic acid is suitable with stearic content, extracellular palm
Acid content is higher than stearic acid；In its metabolite and extra aliphatic acid is not detected by, illustrates that the bacterial strain may be by alkane transformations
To be utilized immediately by thalline after aliphatic acid, the accumulation of organic acid does not occur.
Further, the method for the Acinetobacter calcoaceticus decomposition soil Crude Oil for oil degradation is：Take with
25% glycerine for protective agent in the JH250-8 strains of -80 DEG C of cold storage, rule on LB solid mediums, in 30 DEG C of incubators
Activated strains, good, the larger bacterium colony of selection separation, add in 5ml LB culture mediums, in 30 DEG C, 180rpm shaking table culture 10h,
Transfer by 1% inoculum concentration in 100ml LB culture mediums, in 30 DEG C, 180rpm shaking table culture 10h, by 1% inoculum concentration transfer into
In 2L crude oil culture mediums, in 30 DEG C, 180rpm shaking table cultures 2d；Bacteria concentration OD is adjusted with LB culture mediums600=0.5, it is invested in
In oil-polluted soils, degraded oil under natural conditions, degradation time 30d；Degradation effect detects：Using gravimetric method, oil is taken
Soil after being degraded, boiling range：30-60 DEG C of petroleum ether extraction wherein petroleum component, in steaming petroleum ether in 50 DEG C of water-baths, weighs
Residual oil weight, compared with control group, calculates petroleum degradation rate.
A kind of preparation method of Acinetobacter calcoaceticus for oil degradation, filters out in the soil environment containing crude oil
After the secondary culture of 12 crude oil culture mediums, 30 DEG C of line cultures, take single bacterium colony, continue line separation the Natural strains come
Culture, 4 times repeatedly, takes single bacterium colony for the last time, and Liquid Culture is to OD in LB culture mediums600About 0.5-0.6, adds isometric
50% glycerine, after mixing, packing, -80 DEG C of conservation zone strains；The strain of 5 preservations is taken, send Chinese microorganism strain preservation
Administrative center, applies for culture presevation.
Application of the above-mentioned Acinetobacter calcoaceticus for oil degradation in terms of repairing environment crude oil pollution.
Relative to the prior art, beneficial effects of the present invention are：
A kind of Acinetobacter calcoaceticus for oil degradation of the invention and its preparation method and application.It is of the present invention
Acinetobacter calcoaceticus oil content be 1% under conditions of, in 3 days its oil degradation efficiency (gravimetric method) up to 90% with
On, under the conditions of 5% petroleum concentration, its oil degradation efficiency (gravimetric method) is up to 80% or so in 3 days.There is higher oil degradation
The tolerance of efficiency and high concentration oil.The removing of the method for the present invention and strain to soil Central Plains oily pollution, soil restoration have very
Good effect, has huge environmental protection value and commercial value.It is worth a wide range of to promote.
Brief description of the drawings
Fig. 1 is the different content oil degradation rate schematic diagram of bacterial strain JH250-8.
Fig. 2 is degraded schematic diagrames of the bacterial strain JH250-8 to soil crude oil.
Fig. 3 is opposite degradation rate schematic diagrames of the bacterial strain JH250-8 to soil crude oil.
Fig. 4 is the homology analysis to bacterial strain JH250-8 of the present invention and other bacterial strains, using maximum likelihood method
The evolution tree graph that (Maximum Likelihood method) is drawn.
Embodiment
Technical solution of the present invention is described in further detail with reference to the accompanying drawings and detailed description：
As shown in Figure 1,
Experimental example：
1. microbial name：
Acinetobacter calcoaceticus JH250-8, latin name：Acinetobacter baumannii JH250-8
2. preservation information：
Deposit number:8902；Preservation date：On 03 07th, 2014；Preservation address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1；Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms
Center (CGMCC)
3. microbiologic properties：
(1) substrate situation is utilized：
The bacterial strain decomposable asymmetric choice net alkane derivative, including dodecane, tridecane, the tetradecane, pentadecane, hexadecane, 17
Alkane, octadecane, nonadecane, eicosane, heneicosane, docosane, tricosane, lignocerane, pentacosane, 26
Alkane, heptacosane, octacosane, nonacosane etc..
Containing only the culture medium of crude oil (crude oil is provided by Jilin Jilin Chemical oil plant, picks up from Inner Mongol Manzhouli Area)
In, oil content can be decomposed for more than 90% alkane in 0.6% crude oil culture medium in 3 days.
(2) secretion situation
JH250-8 bacterial strains in normal metabolic processes can the metabolite such as synthetic glycerine, palmitic acid, stearic acid, and three
Kind metabolite can be secreted into extracellular.Wherein glycerine, palmitic acid are higher than intracellular, stearic acid intracellular extracellular concentration in extracellular concentration
It is close.Intracellular palmitic acid is suitable with stearic content, and extracellular palmitic acid content is higher than stearic acid.In its metabolite not
Detect extra aliphatic acid, illustrate that the bacterial strain may not sent out by alkane transformations to be utilized immediately by thalline after aliphatic acid
The accumulation of raw organic acid.
(3) carbon source nitrogen source situation：
The bacterial strain can in most of culture mediums normal growth, using carbon source include monose, polysaccharide (starch), alkane
Hydrocarbon, organic acid and its esters etc., using soluble-carbohydrate as primary carbon source, alkane, aliphatic acid and its derivative do carbon source
When its growth and proliferative speed it is relatively low, be not detected by the utilization to the insolubility polysaccharide such as cellulose.In yeast, beef extract, water
Peptone etc. is solved as well-grown on the culture medium of nitrogen source.
4. applying step：
It is protective agent in the JH250-8 strains of -80 DEG C of cold storage to take using 25% glycerine, is rule on LB solid mediums, in
Activated strains in 30 DEG C of incubators, selection separation is good, and larger bacterium colony, adds in 5ml LB culture mediums, in 30 DEG C, 180rpm
Shaking table culture 10h, transfers in 100ml LB culture mediums by 1% inoculum concentration, in 30 DEG C, 180rpm shaking table culture 10h, by 1%
Inoculum concentration is transferred in 2L crude oil culture mediums, in 30 DEG C, 180rpm shaking table cultures 2d.Bacteria concentration OD is adjusted with LB culture mediums600
=0.5, it is invested in oil-polluted soils, degraded oil under natural conditions, degradation time 30d.Degradation effect detects：Using weight
Amount method, take oil be degraded after soil, petroleum ether (boiling range：30-60 DEG C) extraction wherein petroleum component, steams in 50 DEG C of water-baths
Petroleum ether is removed, weighs Residual oil weight, compared with control group, calculates petroleum degradation rate.
5. application example：
(1) to the degradation of oil in crude oil culture medium
Bacterial strain JH250-8 is inoculated in the crude oil culture medium of various concentrations, degrade 1d, and when 2d, 3d respectively takes three samples,
Petroleum ether extraction petroleum component, after being evaporated petroleum ether, using the oil degradation rate of gravimetric detemination each group.Experimental result confirmation,
Bacterial strain JH250-8 has crude oil stronger degradation capability, can be by 1% oil degradation 90% or so in 2d, can be former by 5% in 3d
Oil degraded nearly 80%.But high concentration crude oil has considerable influence to bacterial strain, its degraded oil ability also declines therewith, such as former 10%
Under oil concentration, JH250-8 bacterial strain 3d degradation rates do not reach 30% (see Fig. 1) yet.Therefore it should be taken into account the bacterial strain pair in practice
The tolerance of petroleum concentration, it is proposed that pollution sources oil content is more suitable 5% or so.Tolerance of the different microorganisms to oil
Sex differernce is larger, it has been reported that microbial degradation rate measure it is more carried out in 0.2-0.5%, and bacterial strain JH250-8 can be 5%
Work under crude oil concentration conditions, it is stronger to illustrate that it is resistant to the ability of oil.
(2) to the degradation of soil petrochina
JH250-8 bacterial strains are cultivated to logarithmic phase (OD in LB culture mediums600About 0.8), by 4：10 (V/W) ratios add
In oil-polluted soils (crude content 6%), degrade 15d under field conditions (factors), and 200g is taken when 2,4,6,8,10,15d
Soil sample, after aeration-drying, takes 100 grams of soil, with 200ml+100ml petroleum ether extractions, merges extract twice, is evaporated
Petroleum ether, using the oil degradation situation of gravimetric analysis each group.JH250-8 groups and the Residual oil measurements of blank group are shown in figure
2.Experiment shows, added in oil-polluted soils JH250-8 bacterium solutions after 4 days petroleum component there is obvious degradation, degradation rate exists
15d or so starts to slow down, and resid amount has obvious correlation (R with the time2=0.92>0.8).And control group (LB culture mediums) group ripple
It is dynamic larger, also acted on without obvious degradation.Using blank group as the opposite drop oil cut rate of reference, analysis shows that, JH250-8 is in natural bar
Start obvious degradation soil Crude Oil, the degradation efficiency highest in the range of 6-10d under part after 4d, degradation rate is in degradation time
Good linear relation (R2=0.98), degradation rate slows down gradually after 10d, and highest is with respect to degradation rate 42.6% (Fig. 3).Bacterial strain
JH250-8 has preferable degradation to soil petrochina component under field conditions (factors), but compared with degradation efficiency in laboratory
Also certain gap, that is to say, that its degradation efficiency can also be improved again.
6. sequencer map (including primer etc.) and phylogenetic tree
Using the genomic DNA for screening bacterium as template, 16S rDNA sequences, Primer are expanded with Bacteria Identification universal primer
And sequence is as follows：
Forward primer：BSF8/20：5’-AGAGTTTGATCCTGGCTCAG-3’
Reverse primer：BSR1541/20：5’-AAGCAGGTCATCCAGCCGCA-3’
PCR response procedures：94℃5min；94 DEG C of 30s, 58 DEG C of 30s, 72 DEG C of 1.5min, 30 circulations； 72℃7min.
Purified with DNA gel extractions kit (AXYGEN companies) to pcr amplification product.The DNA sample of recycling is true
After vacuum freecing-dry, commission Shanghai Ying Jun Bioisystech Co., Ltd carry out determined dna sequence, sequencing primer for BSF8-/
BSR1541。
Arrange and splice through sequence, obtain the 16S rDNA sequences of bacterial strain JH250-8 as shown in SEQ ID No.1.
Sequencing result submits the analysis of online NCBI nucleic acid databases, according to known bacterial strain 16S rDNA sequences in database
Homology determines the biological classification status of bacterial strain JH250-8.Bacterial strain JH250-8 and other bacterial strains are carried out using MEGA7 softwares
Homology analysis, draw chadogram such as Fig. 4 using maximum likelihood method (Maximum Likelihood method)
The above description is merely a specific embodiment, but protection scope of the present invention is not limited thereto, any
The change or replacement expected without creative work, should be covered by the protection scope of the present invention.Therefore, it is of the invention
Protection domain should be determined by the scope of protection defined in the claims.
Sequence table
<110>Beihua University
<120>Acinetobacter calcoaceticus for oil degradation and its preparation method and application
<160>1
<170> PatentIn version 3.5
<210>1
<211>1490
<212>DNA
<213>Acinetobacter baumannii
<400> 1
attgaacgct ggcggcaggc ttaacacatg caagtcgagc ggagagaggt agcttgctac 60
tgatcttagc ggcggacggg tgagtaatgc ttaggaatct gcctattagt gggggacaac 120
atctcgaaag ggatgctaat accgcatacg tcctacggga gaaagcaggg gatcttcgga 180
ccttgcgcta atagatgagc ctaagtcgga ttagctagtt ggtggggtaa aggcctacca 240
aggcgacgat ctgtagcggg tctgagagga tgatccgcca cactgggact gagacacggc 300
ccagactcct acgggaggca gcagtgggga atattggaca atggggggaa ccctgatcca 360
gccatgccgc gtgtgtgaag aaggccttat ggttgtaaag cactttaagc gaggaggagg 420
ctactttagt taatacctag agatagtgga cgttactcgc agaataagca ccggctaact 480
ctgtgccagc agccgcggta atacagaggg tgcaagcgtt aatcggattt actgggcgta 540
aagcgcgcgt aggcggctaa ttaagtcaaa tgtgaaatcc ccgagcttaa cttgggaatt 600
gcattcgata ctggttagct agagtgtggg agaggatggt agaattccag gtgtagcggt 660
gaaatgcgta gagatctgga ggaataccga tggcgaaggc agccatctgg cctaacactg 720
acgctgaggt gcgaaagcat ggggagcaaa caggattaga taccctggta gtccatgccg 780
taaacgatgt ctactagccg ttggggcctt tgaggcttta gtggcgcagc taacgcgata 840
agtagaccgc ctggggagta cggtcgcaag actaaaactc aaatgaattg acgggggccc 900
gcacaagcgg tggagcatgt ggtttaattc gacgcaacgc gaagaacctt acctggcctt 960
gacatagtaa gaactttcca gagatggatt ggtgccttcg ggaacttaca tacaggtgct 1020
gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080
ccttttcctt atttgccagc gagtaatgtc gggaacttta aggatactgc cagtgacaaa 1140
ctggaggaag gcggggacga cgtcaagtca tcatggccct tacggccagg gctacacacg 1200
tgctacaatg gtcggtacaa agggttgcta cacagcgatg tgatgctaat ctcaaaaagc 1260
cgatcgtagt ccggattgga gtctgcaact cgactccatg aagtcggaat cgctagtaat 1320
cgcggatcag aatgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac 1380
catgggagtt tgttgcacca gaagtagcta gcctaactgc aaagagggcg gttaccacgg 1440
tgtggccgat gactggggtg aagtcgtaac aaggtagccg taggggaacc 1490
Claims (8)
1. a kind of Acinetobacter calcoaceticus for oil degradation, it is characterised in that the calcium acetate that this is used for oil degradation is motionless
Bacillus it is entitled：Acinetobacter calcoaceticus JH250-8, latin name：Acinetobacter baumannii JH250-8, are protected
Hiding numbering is:CGMCC No.8902；Preservation date：On 03 07th, 2014；Preservation address：BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3；Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC.
2. a kind of Acinetobacter calcoaceticus for oil degradation according to claim 1, it is characterised in that described to be used for
Its 16S rDNA sequence of the Acinetobacter calcoaceticus of oil degradation is as shown in SEQ ID No.1.
3. a kind of Acinetobacter calcoaceticus for oil degradation according to claim 1, it is characterised in that described to be used for
The Acinetobacter calcoaceticus of oil degradation, decomposable asymmetric choice net alkane derivative, including dodecane, tridecane, the tetradecane, pentadecane,
Hexadecane, heptadecane, octadecane, nonadecane, eicosane, heneicosane, docosane, tricosane, lignocerane, 25
Alkane, hexacosane, heptacosane, octacosane, nonacosane.
4. a kind of Acinetobacter calcoaceticus for oil degradation according to claim 1, it is characterised in that described to be used for
The Acinetobacter calcoaceticus of oil degradation in the culture medium of crude oil is contained only, in 3 days can by oil content be 0.6% crude oil culture
More than 90% alkane decomposes in base.
5. a kind of Acinetobacter calcoaceticus for oil degradation according to claim 1, it is characterised in that described to be used for
The Acinetobacter calcoaceticus of oil degradation, energy synthetic glycerine, palmitic acid, stearic acid are produced as metabolism in normal metabolic processes
Thing, and three kinds of metabolites can be secreted into extracellularly；Wherein glycerine, palmitic acid are higher than intracellular, stearic acid in extracellular concentration
Intracellular extracellular concentration is close；Intracellular palmitic acid is suitable with stearic content, and extracellular palmitic acid content is higher than stearic acid；It is metabolized
In product and extra aliphatic acid is not detected by, it may be sharp by thalline immediately after aliphatic acid by alkane transformations to illustrate the bacterial strain
With the not accumulation of generation organic acid.
6. a kind of Acinetobacter calcoaceticus for oil degradation according to claim 1, it is characterised in that described to be used for
The method that the Acinetobacter calcoaceticus of oil degradation decomposes soil Crude Oil is：It is that protective agent freezes in -80 DEG C to take using 25% glycerine
The JH250-8 strains of Tibetan, rule on LB solid mediums, the activated strains in 30 DEG C of incubators, and selection separation is good, larger
Bacterium colony, add 5ml LB culture mediums in, in 30 DEG C, 180rpm shaking table culture 10h, transfer into 100ml LB by 1% inoculum concentration
In culture medium, in 30 DEG C, 180rpm shaking table culture 10h, transfer by 1% inoculum concentration in 2L crude oil culture mediums, in 30 DEG C,
180rpm shaking table cultures 2d；Bacteria concentration OD is adjusted with LB culture mediums600=0.5, it is invested in oil-polluted soils, natural conditions
Lower degraded oil, degradation time 30d；Degradation effect detects：Using gravimetric method, take oil be degraded after soil, boiling range：30-
60 DEG C of petroleum ether extractions wherein petroleum component, in steaming petroleum ether in 50 DEG C of water-baths, weighs Residual oil weight, compared with control group,
Calculate petroleum degradation rate.
7. a kind of preparation method of Acinetobacter calcoaceticus for oil degradation, screens in the soil environment containing crude oil
Natural strains after the secondary culture of 12 crude oil culture mediums, 30 DEG C line culture, take single bacterium colony, continue line separation training
Support, 4 times repeatedly, take single bacterium colony for the last time, Liquid Culture is to OD in LB culture mediums600About 0.5-0.6, adds isometric
50% glycerine, after mixing, packing, -80 DEG C of conservation zone strains；The strain of 5 preservations is taken, send Chinese microorganism strain preservation
Administrative center, applies for culture presevation.
8. any Acinetobacter calcoaceticus for oil degradation of claim 1-6 is in terms of repairing environment crude oil pollution
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710328505.4A CN107904184A (en) | 2017-05-11 | 2017-05-11 | Acinetobacter calcoaceticus for oil degradation and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710328505.4A CN107904184A (en) | 2017-05-11 | 2017-05-11 | Acinetobacter calcoaceticus for oil degradation and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107904184A true CN107904184A (en) | 2018-04-13 |
Family
ID=61840121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710328505.4A Pending CN107904184A (en) | 2017-05-11 | 2017-05-11 | Acinetobacter calcoaceticus for oil degradation and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107904184A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022165897A1 (en) * | 2021-02-05 | 2022-08-11 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Acinetobacter vivianii, microbial agent thereof and use thereof |
| CN115247140A (en) * | 2022-07-05 | 2022-10-28 | 湖北省生态环境科学研究院(省生态环境工程评估中心) | Bacterial strain for degrading petroleum hydrocarbon under arsenic stress and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914470A (en) * | 2010-07-22 | 2010-12-15 | 山东省科学院生物研究所 | Acinetobacter calcoaceticus and culture method and application thereof |
| CN102146347A (en) * | 2009-12-18 | 2011-08-10 | 浙江天科高新技术发展有限公司 | Acinetobacter sp. and application of composite bacterial agent of acinetobacter species |
| CN102676418A (en) * | 2011-10-28 | 2012-09-19 | 中国科学院烟台海岸带研究所 | Application of acinetobacter calcoaceticus capable of degrading petroleum |
-
2017
- 2017-05-11 CN CN201710328505.4A patent/CN107904184A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146347A (en) * | 2009-12-18 | 2011-08-10 | 浙江天科高新技术发展有限公司 | Acinetobacter sp. and application of composite bacterial agent of acinetobacter species |
| CN101914470A (en) * | 2010-07-22 | 2010-12-15 | 山东省科学院生物研究所 | Acinetobacter calcoaceticus and culture method and application thereof |
| CN102676418A (en) * | 2011-10-28 | 2012-09-19 | 中国科学院烟台海岸带研究所 | Application of acinetobacter calcoaceticus capable of degrading petroleum |
Non-Patent Citations (2)
| Title |
|---|
| WANG,R.J.: "Acinetobacter sp. JH250-8 16S ribosomal RNA gene, partial sequence", 《GENBANK: HQ638093.1》 * |
| 刘五星: "石油污染土壤的生态风险评估和生物修复", 《土壤学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022165897A1 (en) * | 2021-02-05 | 2022-08-11 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Acinetobacter vivianii, microbial agent thereof and use thereof |
| CN115247140A (en) * | 2022-07-05 | 2022-10-28 | 湖北省生态环境科学研究院(省生态环境工程评估中心) | Bacterial strain for degrading petroleum hydrocarbon under arsenic stress and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mino et al. | Sulfurovum aggregans sp. nov., a hydrogen-oxidizing, thiosulfate-reducing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent chimney, and an emended description of the genus Sulfurovum | |
| Chen et al. | Syntrophobacter sulfatireducens sp. nov., a novel syntrophic, propionate-oxidizing bacterium isolated from UASB reactors | |
| Falagán et al. | Acidibacter ferrireducens gen. nov., sp. nov.: an acidophilic ferric iron-reducing gammaproteobacterium | |
| Du et al. | Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov. | |
| Hunter et al. | Rhizobium selenireducens sp. nov.: a selenite-reducing α-Proteobacteria isolated from a bioreactor | |
| Sekiguchi et al. | Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level | |
| Fernández-Martínez et al. | Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax | |
| Slyemi et al. | Characteristics of a phylogenetically ambiguous, arsenic-oxidizing Thiomonas sp., Thiomonas arsenitoxydans strain 3As T sp. nov | |
| Salinas et al. | Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, nitrate-reducing bacterium isolated from an Australian oil well | |
| Li et al. | Biodegradation of crude oil by a newly isolated strain Rhodococcus sp. JZX-01 | |
| Jacques et al. | Characterization of a polycyclic aromatic hydrocarbon–degrading microbial consortium from a petrochemical sludge landfarming site | |
| Suarez et al. | Rheinheimera hassiensis sp. nov. and Rheinheimera muenzenbergensis sp. nov., two species from the rhizosphere of Hordeum secalinum | |
| Viulu et al. | Geobacter luticola sp. nov., an Fe (III)-reducing bacterium isolated from lotus field mud | |
| Thioye et al. | Desulfovibrio senegalensis sp. nov., a mesophilic sulfate reducer isolated from marine sediment | |
| CN107904184A (en) | Acinetobacter calcoaceticus for oil degradation and its preparation method and application | |
| CN105670956A (en) | Medium thermophilic bacillus licheniformis strain and application thereof in treatment on high-temperature oil-bearing wastewater | |
| Ben Ali Gam et al. | Characterization of Desulfovibrio salinus sp. nov., a slightly halophilic sulfate-reducing bacterium isolated from a saline lake in Tunisia | |
| Urakami et al. | Isolation and identification of N, N-dimethylformamide-biodegrading bacteria | |
| Zhang et al. | Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that degrades long-chain fatty acids syntrophically | |
| Sorkhoh et al. | Agarolytic bacteria with hydrocarbon-utilization potential in fouling material from the Arabian Gulf coast | |
| Liu et al. | Thiomicrorhabdus sediminis sp. nov. and Thiomicrorhabdus xiamenensis sp. nov., novel sulfur-oxidizing bacteria isolated from coastal sediments and an emended description of the genus Thiomicrorhabdus | |
| Hamdi et al. | Isolation and characterization of Desulfocurvus thunnarius sp. nov., a sulfate-reducing bacterium isolated from an anaerobic sequencing batch reactor treating cooking wastewater | |
| Choi et al. | Anaerosporomusa subterranea gen. nov., sp. nov., a spore-forming anaerobe belonging to the class Negativicutes isolated from saprolite | |
| Li et al. | Enhanced biodegradation of methyl tert-butyl-ether by a microbial consortium | |
| Selim et al. | Genetic diversity among thermophilic bacteria isolated from geothermal sites by using two PCR typing methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180413 |
|
| WD01 | Invention patent application deemed withdrawn after publication |