CN107898999A - A kind of microorganism formulation containing hedgehog hydnum mycoprotein and its preparation method and application - Google Patents
A kind of microorganism formulation containing hedgehog hydnum mycoprotein and its preparation method and application Download PDFInfo
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- CN107898999A CN107898999A CN201711200432.7A CN201711200432A CN107898999A CN 107898999 A CN107898999 A CN 107898999A CN 201711200432 A CN201711200432 A CN 201711200432A CN 107898999 A CN107898999 A CN 107898999A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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Abstract
The present invention relates to a kind of probiotics and preparation method thereof, more particularly to a kind of microorganism formulation containing hedgehog hydnum mycoprotein and preparation method thereof, by Hericium erinaceus protein extract and compound probiotic according to weight ratio 8:1‑15:1 is uniformly mixed and obtains, and it comprising weight ratio is 12 that the compound probiotic, which is,：1‑2：1‑2：1‑2：1‑2：1‑2：12 Bifidobacteriums, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, the streptococcic mixture of intermediary, the Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, the streptococcic activity of intermediary are 5 X 109‑1ⅹ1010Viable count/g；The preparation method of the Hericium erinaceus protein extract includes：The above-mentioned probiotics of the present invention can significantly improve gastrointestinal function, with it is simple using compound probiotic compound probiotic for example of the present invention compared with, there is significant collaboration booster action.
Description
Technical field
The present invention relates to a kind of probiotics and preparation method thereof, more particularly to a kind of micro- life containing hedgehog hydnum mycoprotein
Thing preparation and its preparation method and application.
Background technology
Hericium erinaceus (Hericium erinaceus) is the edible mushroom of a kind of great nutrition and health care and medical value, mild-natured,
It is sweet in flavor.Research report, Hericium erinaceus active material mainly have polysaccharide, sterol (ergosterol class), terpene (Diterpenes, miscellaneous terpene),
The compounds such as aliphatic acid (saturated fatty acid, unrighted acid etc.), phenols, protein, have relieving the five internal organs, aid digestion, nourishing,
Anticancer, neurasthenia and other effects.
At present, the research both at home and abroad to Hericium erinaceus is concentrated mainly on the side such as extraction, chemical constituents determination of Hericium erinaceus Polysaccharides
Face, but the research to hedgehog hydnum mycoprotein is less.
The content of the invention
The deficiency for more than, the present invention provide a kind of microorganism formulation containing hedgehog hydnum mycoprotein and preparation method thereof.
The present invention reaches above-mentioned purpose by following scheme：
A kind of preparation method of the microorganism formulation containing hedgehog hydnum mycoprotein, by Hericium erinaceus protein extract and compound prebiotic
Bacterium is according to weight ratio 8:1-15:1 is uniformly mixed and obtains, and it comprising weight ratio is 1-2 that the compound probiotic, which is,：1-2：1-2：1-2：
1-2：1-2：The Bifidobacterium of 1-2, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, intermediary's chain
The mixture of coccus, the Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus,
The streptococcic activity of intermediary is respectively 5 X 109-1ⅹ1010Viable count/g；The preparation method of the Hericium erinaceus protein extract
Including：
(1) water of 10%-30% mass is added after Hericium erinaceus is blended, mixing is placed on -80 DEG C of freezing 4-8h,
After Hericium erinaceus completely frost, thaw completely in 37 DEG C of water-baths, multigelation 2-4 times；
(2) NaOH solution is added into the good Hericium erinaceus of freeze thawing, adjusts pH to 9-10, holding solid-liquid ratio is 40-
60ml/g, sealing, magnetic agitation extracts 4-8h under the conditions of 35-40 DEG C, extracts repeatedly twice, filtering, merging filtrate；
(3) pH to 4-5 is adjusted with dilute hydrochloric acid solution, makes precipitation complete, centrifuged, collect precipitation, be that hedgehog hydnum mycoprotein carries
Take thing.
In a preferred embodiment, the preparation method of the Hericium erinaceus protein extract includes：
(1) after Hericium erinaceus is blended with ice crusher add 10%-30% mass distilled water, mixing be placed on-
4-8h is freezed in 80 DEG C of refrigerators, after Hericium erinaceus completely frost, thaws in 37 DEG C of water-baths rapidly, freezes repeatedly completely
Melt 2-4 times；
(2) the good Hericium erinaceus of freeze thawing is put into large beaker, adds NaOH solution, adjust pH to 9-10, kept
Solid-liquid ratio is 40-60ml/g, is sealed with preservative film, and magnetic agitation extracts 4-8h under the conditions of 35-40 DEG C, extracts repeatedly twice,
Filtering, merging filtrate；
(3) pH to 4-5 is adjusted with dilute hydrochloric acid solution, makes precipitation complete, centrifuged, collect precipitation, be that hedgehog hydnum mycoprotein carries
Take thing.
Wherein, the water is distilled water.
Preferably, the step of preparation method of above-mentioned Hericium erinaceus protein extract (2) is：The hedgehog hydnum mushroom good to freeze thawing is real
NaOH solution is added in body, adjusts pH to 9.47, holding solid-liquid ratio is 50ml/g, sealing, the magnetic agitation under the conditions of 37.9 DEG C
6h is extracted, is extracted repeatedly twice, filtering, merging filtrate.
Preferably, in the step of preparation method of above-mentioned Hericium erinaceus protein extract (3), the condition of the centrifugation is：4
Under conditions of DEG C, using the revolution of 8000r/min, centrifugation time 20min.
Preferably, the Bifidobacterium, lactobacillus acidophilus, cheese that it is etc. parts by weight that the compound probiotic is comprising weight ratio
Lactobacillus, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, the streptococcic mixture of intermediary.
Preferably, the Bifidobacterium in the compound probiotic, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, excrement
Streptococcus, galactococcus, the streptococcic activity of intermediary are respectively 1 X 1010Viable count/g.
Preferably, a kind of preparation method of the microorganism formulation containing hedgehog hydnum mycoprotein, by Hericium erinaceus protein extract and
Compound probiotic is according to weight ratio 10:1 be uniformly mixed obtain, the compound probiotic be comprising etc. parts by weight Bifidobacterium,
Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, the streptococcic mixture of intermediary, described is double
Discrimination bacillus, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, the streptococcic activity of intermediary are respectively
For 1 X 1010Viable count/g；The preparation method of the Hericium erinaceus protein extract is as described above.
In the second aspect of the present invention, there is provided micro- life containing hedgehog hydnum mycoprotein that a kind of above-mentioned preparation method is prepared
State preparation.
Probiotics is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, in reproductive system, can produce
Definite health efficacy is main to divide so as to the active beneficial microorganism general name for improving host's microecological balance, playing beneficial effect
For three major types, lactobacillus class, Bifidobacterium class, gram-positive cocci.
In the third aspect of the present invention, there is provided a kind of above-mentioned microorganism formulation containing hedgehog hydnum mycoprotein is improving stomach and intestine work(
Energy, promoting erythrocyte aggegation, anti-inflammatory, raising immunity of organism, the purposes of anti-tumor aspect.
In the fourth aspect of the present invention, there is provided a kind of above-mentioned microorganism formulation containing hedgehog hydnum mycoprotein is preparing improvement stomach
Purposes in terms of gut function, promoting erythrocyte aggegation, anti-inflammatory, raising immunity of organism, antitumor drug or health products.
Extract containing hedgehog hydnum mycoprotein in the present invention has promoting erythrocyte aggegation, anti-inflammatory, improves immunity of organism and resist
The effect of tumour, and compound probiotic promotion intestinal flora structure and abundant aspect there is facilitation.
The above-mentioned probiotics of the present invention can significantly improve gastrointestinal function, and compound probiotic is used with simple
Such as compound probiotic of the present invention is compared, there is significant collaboration booster action.
Brief description of the drawings
Fig. 1 is the Hericium erinaceus Identification of Fusion Protein figure of embodiment 1；
Fig. 2 be test example 1 serum in LPS contents change；
Fig. 3 is the colon pathology slice map of test example 1；
Fig. 4 is ratio chart of each experimental mice intestinal flora of test example 1 in door categorization levels.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
Embodiment 1：
The preparation of Hericium erinaceus protein extract
Comprise the following steps that：
(1) distilled water of 20% mass is added after Hericium erinaceus is blended with ice crusher, mixing is placed on -80 DEG C of ice
6h is freezed in case, after Hericium erinaceus completely frost, is thawed completely in 37 DEG C of water-baths rapidly, multigelation 3 times；
(2) the good Hericium erinaceus of freeze thawing is put into large beaker, adds NaOH solution, adjust pH to 9.47, kept
Solid-liquid ratio is 50ml/g, is sealed with preservative film, and magnetic agitation extracts 6h under the conditions of 37.9 DEG C, extracts twice, filters repeatedly, closes
And filtrate；
(3) pH to 4.5 is adjusted with dilute hydrochloric acid solution, makes precipitation complete, under conditions of 4 DEG C, using turning for 8000r/min
Number, centrifugation time 20min, collects precipitation, is Hericium erinaceus protein extract (HEP).
The Hericium erinaceus protein extract is differentiated, differentiates that collection of illustrative plates is shown in Fig. 1.
Embodiment 2：The preparation of the probiotics of mycoprotein containing hedgehog hydnum and compound probiotic
Take 30 parts by weight of Hericium erinaceus protein extract, Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii,
Streptococcus fecalis, galactococcus, each 3 parts by weight of the streptococcic probiotics dry powder of intermediary, activity are respectively 1.5 X 109Viable count/g,
Be uniformly mixed, dispense to obtain the final product.
Test example 1
SPF grades of BALB/C mices 50 are taken, are randomly divided into 5 groups, every group 10.1 group is left and taken as outside Normal group, remaining
4 groups of equal rectum injection 0.6% trinitrobenzene sulfonic acid (TNBS) (being dissolved in 50% ethanol) 0.1ml prepare Ulcerative Colitis Model.
After modeling success, it is divided into microorganism prepared by HEP groups, compound probiotic group, the embodiment 2 of HEP+ compound probiotics
Preparation group, model group.Except normal group is outer, other groups give a large amount of antibiotic treatments, and corresponding medicine is given by group, model group with
Normal group gavage distilled water.After continuing ten days, mouse is put to death, takes blood, small intestine, excrement.Related test results are shown in Fig. 2, Fig. 3, figure
4。
The horizontal measurement results of LPS in serum are shown from Fig. 2, model group content is 210% normally organized.HEP is administered
After composite probiotics preparations, LPS levels are all decreased obviously in mice serum, after HEP is used in conjunction with compound probiotic, blood
LPS contents only increase by 20% or so in clear, have synergistic function.
It can be seen that from the colon pathological section of Fig. 3 compared with blank control group, model group is presented intestinal mucosa and diffuses
Simultaneously there is crack shape ulcer lesion in uniformity inflammatory phenomena, intestinal mucosa structural change.After HEP and compound probiotic is administered, intestines glue
Membrane structure is more regular, and crack shape ulcer changes also less.
As can be seen from Figure 4：Compared with normal group, mouse is after TNBS is handled, actinomyces door in enteron aisle
(Actinobacteria), Firmicutes (Firmicutes) and TM7 bacteriums are increased, deferrization bacillus door
(Deferribacteres) reduced with Proteobacteria (Proteobacteria)；Compared with TNBS groups, administration HEP, compound prebiotic
After the microorganism formulation of bacterium and HEP+ compound probiotics, Bacteroidetes (Bacteroidetes) in mouse intestinal, Firmicutes and
Proteobacteria bacterium number showed increased；After a large amount of antibiotic treatments, deferrization bacillus door, Proteobacteria and TM7 bacteriums are almost
Killed completely, and each Bacteriophyta is then maintained structural integrity after HEP, compound probiotic and HEP+ compound probiotics is administered.
To sum up it can be seen that, after ulcerative colitis mouse takes HEP, compound probiotic, HEP+ compound probiotics, and just
Often group has also obtained obvious improvement compared to the symptom index of all ulcerative colitis, and particularly HEP+ compound probiotic groups change
It is kind to become apparent, there is synergistic function.
The above, is only the preferable specific embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Design is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (9)
1. a kind of preparation method of the microorganism formulation containing hedgehog hydnum mycoprotein, it is characterised in that by Hericium erinaceus protein extract
With compound probiotic according to weight ratio 8:1-15:1 is uniformly mixed and obtains, and it comprising weight ratio is 1-2 that the compound probiotic, which is,：1-
2：1-2：1-2：1-2：1-2：1-2 Bifidobacteriums, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, milk-globule
Bacterium, the streptococcic mixture of intermediary, the Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, excrement hammer
Bacterium, galactococcus, the streptococcic activity of intermediary are respectively 5 X 109-1ⅹ1010Viable count/g；The Hericium erinaceus protein extract
Preparation method include：
(1) water of 10%-30% mass is added after Hericium erinaceus is blended, mixing is placed on -80 DEG C of freezing 4-8h, treats monkey
After the frost completely of head mushroom entity, thaw completely in 37 DEG C of water-baths, multigelation 2-4 times；
(2) NaOH solution is added into the good Hericium erinaceus of freeze thawing, adjusts pH to 9-10, holding solid-liquid ratio is 40-60ml/
G, sealing, magnetic agitation extracts 4-8h under the conditions of 35-40 DEG C, extracts repeatedly twice, filtering, merging filtrate；
(3) pH to 4-5 is adjusted with dilute hydrochloric acid solution, makes precipitation complete, centrifuged, collect precipitation, be Hericium erinaceus protein extract.
A kind of 2. preparation method of microorganism formulation containing hedgehog hydnum mycoprotein according to claim 1, it is characterised in that
The preparation method of the Hericium erinaceus protein extract includes：
(1) distilled water of 10%-30% mass is added after Hericium erinaceus is blended with ice crusher, mixing is placed on -80 DEG C
4-8h is freezed in refrigerator, after Hericium erinaceus completely frost, is thawed completely in 37 DEG C of water-baths rapidly, multigelation 2-
4 times；
(2) the good Hericium erinaceus of freeze thawing is put into large beaker, adds NaOH solution, adjust pH to 9-10, keep feed liquid
Than for 40-60ml/g, being sealed with preservative film, magnetic agitation extracts 4-8h under the conditions of 35-40 DEG C, extracts repeatedly twice, filtering,
Merging filtrate；
(3) pH to 4-5 is adjusted with dilute hydrochloric acid solution, makes precipitation complete, centrifuged, collect precipitation, be Hericium erinaceus protein extract.
3. a kind of preparation method of microorganism formulation containing hedgehog hydnum mycoprotein according to claim 1 or 2, its feature exist
(2) are in, the preparation method of above-mentioned Hericium erinaceus protein extract the step of：NaOH is added in the Hericium erinaceus good to freeze thawing
Solution, adjusts pH to 9.47, and holding solid-liquid ratio is 50ml/g, and sealing, magnetic agitation extracts 6h under the conditions of 37.9 DEG C, carries repeatedly
Take twice, filtering, merging filtrate.
4. a kind of preparation method of microorganism formulation containing hedgehog hydnum mycoprotein according to claim 1 or 2, its feature exist
In, the preparation method of above-mentioned Hericium erinaceus protein extract the step of in (3), the condition of the centrifugation is：Under conditions of 4 DEG C,
Using the revolution of 8000r/min, centrifugation time 20min.
A kind of 5. preparation method of microorganism formulation containing hedgehog hydnum mycoprotein according to claim 1, it is characterised in that
It comprising weight ratio the Bifidobacteriums of parts by weight, lactobacillus acidophilus, Lactobacillus casei, the Zhan Shi breast bars such as is that the compound probiotic, which is,
Bacterium, streptococcus fecalis, galactococcus, the streptococcic mixture of intermediary.
A kind of 6. preparation method of microorganism formulation containing hedgehog hydnum mycoprotein according to claim 1, it is characterised in that
Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus in the compound probiotic,
The streptococcic activity of intermediary is respectively 1 X 1010Viable count/g.
A kind of 7. preparation method of microorganism formulation containing hedgehog hydnum mycoprotein according to claim 1, it is characterised in that
By Hericium erinaceus protein extract and compound probiotic according to weight ratio 10:1 be uniformly mixed obtain, the compound probiotic be comprising
Weight ratio is 1:1 Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, intermediary's hammer
The mixture of bacterium, the Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, streptococcus fecalis, galactococcus, in
The streptococcic activity that is situated between is respectively 1 X 1010Viable count/g.
It is 8. a kind of according to micro- life containing hedgehog hydnum mycoprotein that any preparation method is prepared in claim 1 to 7
State preparation.
9. the probiotics containing hedgehog hydnum mycoprotein described in a kind of claim 9 is red thin in preparation improvement gastrointestinal function, rush
Purposes in terms of born of the same parents' aggegation, anti-inflammatory, raising immunity of organism, antitumor drug or health products.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109096365A (en) * | 2018-09-20 | 2018-12-28 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of Hericium erinaceus protein extract and compound adjunct antineoplastic preparation and their preparation method and purposes |
| CN110304737A (en) * | 2019-07-09 | 2019-10-08 | 常德市万惠生物科技有限公司 | A kind of compound probiotic and preparation method thereof |
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| CN104382955A (en) * | 2014-12-02 | 2015-03-04 | 汤臣倍健股份有限公司 | Composition, application thereof and health product |
| CN104825570A (en) * | 2015-05-20 | 2015-08-12 | 杨克西 | Composition for inhibiting helicobacter pylori and application of composition |
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| CN104382955A (en) * | 2014-12-02 | 2015-03-04 | 汤臣倍健股份有限公司 | Composition, application thereof and health product |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109096365A (en) * | 2018-09-20 | 2018-12-28 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of Hericium erinaceus protein extract and compound adjunct antineoplastic preparation and their preparation method and purposes |
| CN110304737A (en) * | 2019-07-09 | 2019-10-08 | 常德市万惠生物科技有限公司 | A kind of compound probiotic and preparation method thereof |
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