CN107897269A - A kind of coarse cereal biscuit of peptide containing fish protein and preparation method thereof - Google Patents

A kind of coarse cereal biscuit of peptide containing fish protein and preparation method thereof Download PDF

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Publication number
CN107897269A
CN107897269A CN201711367052.2A CN201711367052A CN107897269A CN 107897269 A CN107897269 A CN 107897269A CN 201711367052 A CN201711367052 A CN 201711367052A CN 107897269 A CN107897269 A CN 107897269A
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China
Prior art keywords
preparation
biscuit
coarse cereal
minced
enzymolysis
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CN201711367052.2A
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CN107897269B (en
Inventor
李新
汪兰
熊光权
吴文锦
丁安子
乔宇
廖李
石柳
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Farm Product Processing and Nuclear Agricultural Technology Institute of Hubei Academy of Agricultural Sciences
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Farm Product Processing and Nuclear Agricultural Technology Institute of Hubei Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00Finished or partly finished bakery products
    • A21D13/06Products with modified nutritive value, e.g. with modified starch content
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/24Organic nitrogen compounds
    • A21D2/26Proteins
    • A21D2/268Hydrolysates from proteins
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/36Vegetable material

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

The invention belongs to field of food, and in particular to a kind of coarse cereal biscuit of peptide containing fish protein and preparation method thereof.The preparation method includes:Using minced fish as raw material, add water and protease digests minced fish, after enzymolysis, be warming up to 90~100 DEG C, keep the temperature 30~60min, filter to take supernatant;Coarse cereal powder, Self- raising flour, minced fillet enzymolysis liquid, vegetable oil, dusty yeast, salt and sodium bicarbonate is taken to obtain dough after stirring evenly;Dough is placed in and is fermented under room temperature, subsequent compression molding, baked again obtain the multigrain biscuit of peptide containing fish protein.The present invention is using one or more single compounding coarse cereals as raw material, minced fillet enzymolysis liquid is added in the feed, the coarse cereal biscuit of peptide containing fish protein not only has preferable processing performance, with preferable hardness, toughness and roughness, and the coarse cereal biscuit has significant antioxidation also rich in nutritional ingredients such as protein peptides, amino acid.

Description

A kind of coarse cereal biscuit of peptide containing fish protein and preparation method thereof
Technical field
The invention belongs to field of food field, and in particular to a kind of coarse cereal biscuit of peptide containing fish protein and preparation method thereof.
Background technology
China's coarse cereals resource very abundant, kind is more, is broadly divided into four major classes, such as cereals (including buckwheat, swallow Wheat, black rice etc.), miscellaneous beans (including mung bean, red bean, pea etc.), potato (potato, sweet potato, Chinese yam etc.) and oil Class (including Sesame, walnut, oily certain herbaceous plants with big flowers etc.).Coarse cereals fabricated product is mainly that primary processed goods is made in simple screening at present, or passes through xeraphium The process such as broken is processed into instant food, and intensive processing product is less, and added value of product is not high.In coarse cereals protein composition and Structure is different from wheat gluten, is difficult to form net structure in process, so that it cannot receive and retain substantial amounts of moisture, Protein quality is coarse, ductility is not good enough, elastic deficiency so as to causing, therefore dough characters is poor, is not suitable for processing and fabricating wheaten food Product.In addition coarse cereal biscuit in process there is also molding effect, mouthfeel taste is bad and nutritional ingredient is not comprehensive etc. asks Topic.
The content of the invention
The present invention is in view of the deficiencies of the prior art, and it is an object of the present invention to provide a kind of coarse cereal biscuit of peptide containing fish protein and its preparation side Method.
For achieving the above object, the technical solution adopted by the present invention is:
A kind of preparation method of the coarse cereal biscuit of peptide containing fish protein, includes the following steps:
(1) crushed after coarse cereals essence is picked up by pulverizer, it is spare to cross 30~40 mesh dusting covers;
(2) preparation of fish protein enzymolysis liquid:Using minced fish as raw material, add water and protease and minced fish is carried out Digest, after enzymolysis, be warming up to 90~100 DEG C, keep the temperature 30~60min, it is spare to filter to take supernatant;
(3) count in parts by weight, take 40~50 parts of coarse cereal powder, 15~20 parts of Self- raising flour, 25~30 parts of minced fillet enzymolysis liquid, 0.5~1 part of 3~5 parts of vegetable oil, 0.5~1 part of dusty yeast, 0.5~1 part of salt and sodium bicarbonate;Egg containing the flesh of fish is obtained after stirring evenly The dough of white peptide;
(4) dough is placed in and fermented under room temperature, after fermentation, compression molding;It is finally baked to obtain containing the flesh of fish Protein peptides multigrain biscuit.
In such scheme, step (2) described protease is animal proteolytic enzyme and flavor protease, the animal protein The enzyme activity of hydrolase is 100,000 u/g, and the enzyme activity of the flavor protease is 30,000 u/g, and the additive amount of the protease is fresh-water fishes The 0.2%~0.3% of rotten quality.
In such scheme, the solid-to-liquid ratio of step (2) minced fish and water is 1:1.5~1:2.
In such scheme, the reaction temperature of step (2) described enzymolysis is 55~60 DEG C, and enzymolysis time is 5~6h.
In such scheme, the time of step (4) described fermentation is 1h~1.5h.
In such scheme, step (4) temperature baked is 170 DEG C, and the time baked is 15~20min.
Beneficial effects of the present invention are as follows:The present invention is replaced using one or more single compounding coarse cereals as raw material with minced fillet enzymolysis liquid Dai Shui is added in dough, using crushing, dispensing, is fermented, the process exploitation coarse cereal biscuit of peptide containing fish protein such as is baked;It is described to contain Fish protein peptide coarse cereal biscuit not only has a preferable processing performance, good forming effect, coarse cereal biscuit have preferable hardness, toughness and Roughness, and the coarse cereal biscuit is good in taste, rich in nutritional ingredients such as protein peptides, amino acid, there is significant antioxygen to be turned into With.
Brief description of the drawings
Fig. 1 is amino-acid nitrogen content under different hydrolysis times.
Fig. 2 is different hydrolysis time degree of hydrolysis.
Fig. 3 is different hydrolysis time protein recoveries.
Fig. 4 is the DPPH clearance rates of different hydrolysis times.
Fig. 5 is the O2- clearance rates of different hydrolysis times.
Fig. 6 is the reducing power of different hydrolysis times.
Fig. 7 is the OH clearance rates of different hydrolysis times.
Embodiment
For a better understanding of the present invention, with reference to the embodiment content that the present invention is furture elucidated, but the present invention Content is not limited solely to the following examples.
Embodiment 1
The preparation of fish protein enzymolysis liquid:Using minced fish as raw material, add water and protease and enzyme is carried out to minced fish Solve, after enzymolysis, be warming up to 100 DEG C, keep the temperature 60min, filter to take supernatant;The protease is animal proteolytic enzyme (100,000 u/g) and flavor protease (30,000 u/g), the additive amount of protease are the 0.2% of minced fish quality;The enzymolysis Reaction temperature is 55~60 DEG C, and enzymolysis time is 1~6h.
The present embodiment, which tests, prepares amino peptide nitrogen content, albumen in gained fish protein enzymolysis liquid under different hydrolysis times Degree of hydrolysis, protein recovery, DPPH clearance rates, O2- clearance rate, OH clearance rates and reducing power, as a result as shown in figs. 1 to 6, Fig. 1 ~3 illustrate minced fillet through 5~6h of protease hydrolyzed, and amino nitrogen, degree of hydrolysis, protein recovery reach peak in enzymolysis liquid; Fig. 4~Fig. 6 illustrates that minced fillet has certain inoxidizability through 1~6h of protease hydrolyzed, enzymolysis liquid small molecular peptide.
The present embodiment also measured were free aminoacid content in minced fillet protein enzymatic hydrolyzate, and the results are shown in Table 1, and table 1 illustrates For minced fillet after protease hydrolyzed 5h, free aminoacid content reaches 760.53mg/100g in enzymolysis liquid, enzymolysis liquid is added to In coarse cereal powder, free aminoacid content in coarse cereal biscuit can be greatly improved, impart protein peptides in coarse cereal biscuit, amino acid nutrient into Point.
Free aminoacid content in 1 fish protein enzymolysis liquid of table
Amino acid classes Content mg/100ml
Threonine 19.76
Glutamic acid 31.31
Glycine 4.00
Alanine 19.56
Cystine 22.16
Valine 40.93
Methionine 33.00
Isoleucine 25.08
Leucine 126.18
Tyrosine 75.88
Phenylalanine 102.90
Ornithine 17.38
Lysine 122.24
Proline 3.40
Histidine 13.93
Arginine 102.82
Summation 760.53
Embodiment 2
A kind of preparation method of the coarse cereal biscuit of peptide containing fish protein, includes the following steps:
(1) crushed after coarse cereals essence is picked up by pulverizer, it is spare to cross 30~40 mesh dusting covers;
(2) preparation of fish protein enzymolysis liquid:Using minced fish as raw material, add water and protease and minced fish is carried out Digest, after enzymolysis, be warming up to 90~100 DEG C, keep the temperature 30~60min, it is spare to filter to take supernatant;The protease is Thing proteolytic enzyme (100,000 u/g) and flavor protease (30,000 u/g), the additive amount of protease is minced fish quality 0.2%;The solid-to-liquid ratio of the minced fish and water is 1:1.5~1:2;The reaction temperature of the enzymolysis is 55~60 DEG C, enzymolysis Time is 5~6h;
(3) count in parts by weight, take 45.52 parts of coarse cereal powder, 18.21 parts of Self- raising flour, 30.35 parts of minced fillet enzymolysis liquid, plant 0.76 part of 3.79 parts of thing oil, 0.61 part of dusty yeast, 0.76 part of salt and sodium bicarbonate;The face of the peptide containing fish protein is obtained after stirring evenly Group;
(5) dough is placed in the 1h that ferments under room temperature, after fermentation, compression molding obtains fermentation biscuit;Finally will Fermentation biscuit obtains the multigrain biscuit of peptide containing fish protein after baking;The temperature baked is 170 DEG C, the time baked for 15~ 25min。
The present embodiment has investigated fermented dough compared with non-fermented dough texture characteristic, ferment biscuit and biscuit matter of not fermenting Structure compares, the multigrain biscuit of peptide containing fish protein compares with baking time texture, as a result table 2, table 3, shown in table 4, from 2~table of table 4 Understand:After 1~1.5h of dough fermentation, the index such as the hardness of dough, viscosity, elasticity, gumminess, chewiness is superior to not ferment The index such as dough, the hardness of the fermentation biscuit that dough compression molding obtains, toughness, roughness is also superior to non-fermented dough shaping Biscuit, as shown in Table 4:Baking time in 15~20min, obtain the multigrain biscuit of peptide containing fish protein have it is preferable hard Degree, toughness and roughness.It follows that the coarse cereal biscuit of peptide containing fish protein has a preferable processing performance, good forming effect, It is good in taste with preferable hardness, toughness and roughness, and rich in nutritional ingredients such as protein peptides, amino acid, have significant Antioxidation.
2 fermented dough of table is compared with non-fermented dough texture characteristic
Index Hardness/g Viscosity/g.sec Recovery/% Cohesion/% Elasticity/% Gumminess Chewiness
Fermented dough 7866.59 -65.40 9.65 0.22 35.79 1723.10 604.78
Non- fermented dough 3531.46 -169.80 4.85 0.18 27.36 618.60 170.11
Table 3 ferments biscuit compared with biscuit texture of not fermenting
Index Maximum penetration hardness/g Puncture acting (toughness)/g.mm Biscuit roughness Puncture broken linear range/g.sec
Ferment biscuit 1304.37 1480.70 14.40 5704.07
Do not ferment biscuit 1243.29 1987.06 30.60 6966.65
4 multigrain biscuit of peptide containing fish protein of table compares with baking time texture
Baking time/min 5 10 15 20 25
Maximum pierces through hardness average/g 51.43 150.59 866.26 824.32 1069.44
Puncture acting (toughness)/g.mm 350.86 1089.79 1294.80 1190.33 1236.32
Biscuit roughness 2.00 4.40 15.40 16.40 15.80
Puncture broken linear range/g.sec 4751.40 5271.72 5101.88 4785.30 4777.38
Obviously, above-described embodiment is only intended to clearly illustrate made example, and is not the limitation to embodiment.It is right For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change or change therefore amplified Move within still in the protection domain of the invention.

Claims (6)

1. a kind of preparation method of the coarse cereal biscuit of peptide containing fish protein, it is characterised in that include the following steps:
(1)Crushed after coarse cereals essence is picked up by pulverizer, it is spare to cross 30 ~ 40 mesh dusting covers;
(2)The preparation of fish protein enzymolysis liquid:Using minced fish as raw material, add water and protease and enzyme is carried out to minced fish Solve, after enzymolysis, be warming up to 90 ~ 100 DEG C, keep the temperature 30 ~ 60 min, it is spare to filter to take supernatant;
(3)Count in parts by weight, take 40 ~ 50 parts of coarse cereal powder, 15 ~ 20 parts of Self- raising flour, 25 ~ 30 parts of minced fillet enzymolysis liquid, vegetable oil 3 0.5 ~ 1 part of ~ 5 parts, 0.5 ~ 1 part of dusty yeast, 0.5 ~ 1 part of salt and sodium bicarbonate;The dough of the peptide containing fish protein is obtained after stirring evenly;
(4)Dough is placed in and is fermented under room temperature, after fermentation, compression molding;It is finally baked to obtain containing fish protein Peptide multigrain biscuit.
2. preparation method according to claim 1, it is characterised in that step(2)The protease is animal proteolytic enzyme And flavor protease, the enzyme activity of the animal proteolytic enzyme is 100,000 u/g, and the enzyme activity of the flavor protease is 30,000 u/g, The additive amount of the protease is the 0.2% ~ 0.3% of minced fish quality.
3. preparation method according to claim 1, it is characterised in that step(2)The solid-to-liquid ratio of the minced fish and water is 1:1.5~1:2。
4. preparation method according to claim 1, it is characterised in that step(2)The reaction temperature of the enzymolysis is 55 ~ 60 DEG C, enzymolysis time is 5 ~ 6h.
5. preparation method according to claim 1, it is characterised in that step(4)The time of the fermentation is 1h ~ 1.5h.
6. preparation method according to claim 1, it is characterised in that step(4)The temperature baked is 170 DEG C, is baked Time be 15 ~ 20 min.
CN201711367052.2A 2017-12-18 2017-12-18 Coarse cereal cake containing fish protein peptide and preparation method thereof Active CN107897269B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108835185A (en) * 2018-09-10 2018-11-20 张家界金鲵生物工程股份有限公司 A kind of production method and products thereof of the coarse cereal biscuit of protein peptides containing giant salamander
CN115226862A (en) * 2022-06-23 2022-10-25 北京宝贝优鲜科技有限公司 Fish double-protein peptide and preparation method and composition thereof

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CN104855479A (en) * 2015-05-13 2015-08-26 浙江工业大学 Recombinant fish cookie and processing method therefor
CN106172634A (en) * 2016-07-06 2016-12-07 武汉好多多生物科技有限公司 A kind of processing method of nutrient biscuit
CN106261589A (en) * 2016-08-04 2017-01-04 刘良忠 Special dietary seafood that a kind of applicable patients with chronic diseases is edible and processing method thereof
CN106804668A (en) * 2017-04-06 2017-06-09 刘华 Dandelion multigrain biscuit and preparation method thereof

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CN104855479A (en) * 2015-05-13 2015-08-26 浙江工业大学 Recombinant fish cookie and processing method therefor
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CN106261589A (en) * 2016-08-04 2017-01-04 刘良忠 Special dietary seafood that a kind of applicable patients with chronic diseases is edible and processing method thereof
CN106804668A (en) * 2017-04-06 2017-06-09 刘华 Dandelion multigrain biscuit and preparation method thereof

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108835185A (en) * 2018-09-10 2018-11-20 张家界金鲵生物工程股份有限公司 A kind of production method and products thereof of the coarse cereal biscuit of protein peptides containing giant salamander
CN115226862A (en) * 2022-06-23 2022-10-25 北京宝贝优鲜科技有限公司 Fish double-protein peptide and preparation method and composition thereof

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