CN107868764A - A kind of compound probiotic powder preparation for strengthening immunity of pets and preparation method thereof - Google Patents
A kind of compound probiotic powder preparation for strengthening immunity of pets and preparation method thereof Download PDFInfo
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- CN107868764A CN107868764A CN201711208944.8A CN201711208944A CN107868764A CN 107868764 A CN107868764 A CN 107868764A CN 201711208944 A CN201711208944 A CN 201711208944A CN 107868764 A CN107868764 A CN 107868764A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
Abstract
The invention belongs to compound probiotic powder preparation field, more particularly to a kind of compound probiotic powder preparation for strengthening immunity of pets, excellent prebiotic strain bifidobacterium longum BB, animal bifidobacteria V9, Lactobacillus plantarum PP12, lactobacillus acidophilus NM are carried out formulation optimization and compound probiotic powder are made by the formula, acted synergistically using the uniqueness between strain, it is high to be not only colonized power, reduce the absorption to cholesterol, and the gastro-intestinal digestion tract disease of pet can be improved, strengthen immunologic cellular activity, have obvious facilitation particularly with the raising of immunity of organisms.
Description
Technical field
The invention belongs to compound probiotic powder preparation field, and in particular to a kind of compound probiotic for strengthening immunity of pets
Powder preparation.
Background technology
The microorganism in terms of trillion is counted in animal intestinal tract and constitutes gut flora, these microorganisms and animal body normal generation
Thank it is closely related, once Bacterial community occur imbalance or destroyed, various metabolic diseases are following.Different enteric microorganism
Structure and composition affects host's absorption of nutrient ingredients, energy balance, immunocompetence and other a variety of important physiological activities.Grind
Study carefully and show, the intestinal microflora of disorder can be adjusted by probiotic supplemented and improve the immunocompetence of body, and then improved
Body health is horizontal.
In order to improve pet alimentary canal bacteria planting, promote gastrointestinal peristalsis, while strengthen the specific and non-specific of body
Property immunologic function, improve the immunity of organisms and premunition of pet.Therefore, we develop special enhancing pet immunity of organism
Power, the compound probiotic powder preparation of high vigor is easily colonized, including following several probiotics, including following several prebiotic
Bacterium.
Bifidobacterium longum BB, its characteristic are as follows：Bifidobacterium longum BB is strict anaerobes, is directly supplemented with by exogenous
Beneficial bacteria, intestinal pH can be reduced, suppress the growth and breeding of pathogenic bacteria, reached regulation intestinal bacilli illness and improve intestinal microenvironment
Effect.Bifidobacterium tends to utilize oligosaccharides or monose in enteron aisle, and starch is Bifidobacterium in enteron aisle in polysaccharide
Advantage substrate, content of the starch polysaccharide in enteron aisle are the key constraints of Bifidobacterium abundance.Bifidobacterium longum has
The complete approach of maltodextrin metabolism, lack the complete metabolic pathway that plant cell wall polysaccharides are substrate, it is impossible to directly utilize intestines
Common cell wall polysaccharides in road, maltodextrin present in enteron aisle can be utilized；And contain malt in probiotics preparation formula
Dextrin, and we select a reason of bifidobacterium longum.At it using edible middle addition Bifidobacterium, bifid can be accelerated
The speed of bacillus intestinal canal colonization, and reduce the probability that diarrhoea and infection of digestive canal occurs；Substantial amounts of research is also demonstrated that in enteron aisle
The abundance of Bifidobacterium is closely related with body health, and abundance of the Bifidobacterium in enteron aisle is by as a kind of intestinal health and people
The important indicator of body health.Bifidobacterium longum can have enhancing immune response, reduce intestines problem time of origin and reduce generation
Frequency, reduce the effect of serum cholesterol is definite.Although the primary stage of the research of the prebiotic mechanism of Bifidobacterium, using external and
Zoopery preliminary proof Bifidobacterium is played a role, and except the metabolite of itself, Bifidobacterium can also be produced by being metabolized
The reciprocation of thing promotes the main path of the growth of beneficial bacterium to include：Strengthen gut barrier function, adjust immune response, suppress
Pathogenic bacteria etc..Fructose or acetic acid are provided, so as to increase the generation of butyric acid in enteron aisle, promote the health of enteron aisle.
Animal bifidobacteria V9, its characteristic are as follows：After Bifidobacterium V9 can digest 3h in pH3.0 simulated gastric fluid,
Survival rate still reaches more than 90%；After Bifidobacterium V9 can digest 5h in pH8.0 simulated intestinal fluid, survival rate still reaches
More than 95%；To enteron aisle lead to diarrhea bacterial strain Shigella shigae, salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa
There is different degrees of antagonism；DNA is not present in bacterial strain, is also just not present in enteron aisle and propagates Drug Resistance Plasmidss
Risk；With immunoregulation effect, animal experiment shows, after animal takes 10 days, with challenge with E.coli, prevention effective percentage reaches
97.5%, the control group death rate is up to 50%.After using Bifidobacterium V9 preparations in feeding period, average weight gain improves 10.51%,
Experiment collection excrement sample, detects the viable count of Bifidobacterium and Escherichia coli, as shown by data, and Bifidobacterium V9 preparations can significantly promote
Enter the propagation of Bifidobacteria population in enteron aisle, suppress the growth of Escherichia coli.
Lactobacillus plantarum PP12, its characteristic are as follows：Nose circle straight-bar bacterium, usually 3.0~8.0 μm of 0.9~1.2 μ m, single
Individual, paired or short chain shape.Flagellum is generally deficient of, but can be moved.Gram-positive, spore of not sprouting.Amphimicrobian, surface colony are straight
Footpath about 3mm, raised, rounded, surface is smooth, fine and closely woven, and color is white, once in a while in pale yellow or buff.Belong to chemoheterotrophic bacteria, growth needs
Nutritious culture medium is wanted, it is necessary to calcium pantothenate and nicotinic acid, but does not need thiamine, pyridoxal or pyridoxamine, folic acid, vitamin
B12.Can ferment pentoses or gluconate, 85% above is lactic acid in end-product.Nitrate is not generally reduced, is not liquefied bright
Glue, catalase and oxidizing ferment are all negative.D, Pfansteihl can be produced, there is fructose 1,6-diphosphate aldolase and hexose monophosphate approach
Activity, can be grown in grape hydrochlorate, and produce C02.Ferment the ribose of 1 molecule or the lactic acid of other pentoses 1 molecule of generation
With the acetic acid of 1 molecule.15 DEG C can grow, and usual optimum growth temperature is 30~35 DEG C.Lactobacillus plantarum is one kind of lactic acid bacteria,
Optimum growth temperature is 30~35, anaerobism or amphimicrobian, and strain is single, sometimes in pairs or into chain to be straight or curved shaft-like,
Optimal pH 6.5 or so, belongs to homofermentative lactic bacteria.Compared with this bacterium is the viable count of this bacterium with the difference of other lactic acid bacterias
Height, acid can be largely produced, the pH stable in water is not raised, and the acidic materials of its output can degrade heavy metal；Due to
This bacterium is anaerobic bacteria (facultative aerobic), and the energy distinctive lactobacillin of output in reproductive process, lactobacillin is a kind of
The preservative of bion.
Lactobacillus acidophilus NM, its characteristic are as follows：It belongs to lactobacillus, and Gram-positive bacillus, bar end is rounded, it
Not only under one's belt, and it is primarily present in small intestine, lactic acid can be discharged, acetic acid works with some other to harmful bacteria
Antibiotic, antagonism is produced to pathogenic entero becteria, but bacteriostasis is relatively weak.
This compound probiotic powder is mainly characterized by, by excellent prebiotic strain bifidobacterium longum BB, animal bifidobacteria
V9, Lactobacillus plantarum PP12, lactobacillus acidophilus NM carry out formulation optimization and compound probiotic powder are made, and this formula is tested by experiment
Card, the inoculating proportion of science is worked out, acted synergistically using the uniqueness between strain, be not only colonized power height, reduce to cholesterol
Absorption, and the gastro-intestinal digestion tract disease of pet can be improved, strengthen immunologic cellular activity, the body doted on particularly with old young disease
The raising of immunity has obvious facilitation.And two kinds of unique Bifidobacteriums are added in product, the intestines where Bifidobacterium
Road flora has complicated flora composition, is 10 times of body cell, contained number gene is 100 times of human body gene number.
Bifidobacterium forms symbiosis or the competitive relation of complexity with these floras.Enteron aisle is the key position of body nutrient absorption, nutrition
Absorption requires that enteron aisle has big surface area and thin top layer；Simultaneously because the presence of a large amount of floras and exogenous antigen, enteron aisle must
Pathogenic thalline and antigen need to be prevented to enter, this functional requirement enteron aisle has more firm structure.Powerful immunologic function is
Solves the central genetic strategy of host's resistivity and both nutrient absorption functions contradiction.Intestinal tract immune system is viscous by secreting
Liquid, antibacterial material forms the physical barriers on surface, while the subepidermal immunocyte of enteron aisle is by gulping down saliva, killing, antibody response
And the exotic antigen and thalline that the removing such as cell factor enters.The even more important function of intestinal tract immune system is be effective against
Immune silence is kept while exotic antigen to commensal gut flora.This adaptability to intestinal tract immune system of gut flora
It is the significant challenge that bacterial strain is colonized in enteron aisle.This compatibility of gut flora and host immune system is mainly thin by host
Identification of the born of the same parents to phage surface composition is realized.The approach of bacterial strain resistance host immune response mainly has two kinds：Produce surface
Protective substances, such as polysaccharide；The adhesion factor that can extend is produced, bacterial strain is protected certain distance with host, such as flagellum, pili
Deng.Research shows that Lactobacillus plantarum exocellular polysaccharide reduces the immune response of bacterial strain.Surface polysaccharide protection bacterial strain resistance intestine immunity
Reaction, colonization ability of the increase bacterial strain in enteron aisle.Adaptation of the Bifidobacterium to intestinal environment is related to the acquisition energy to polysaccharide
Power, to the adhesive capacity of enteron aisle, to the adaptability of intestine immunity environment and ionic environment, and it is also possible that numerous unknown
Factor, the competition by complicated gut flora and symbiosis are influenceed in addition so that this research turn into one it is of crucial importance
Process.
Unlike the production method of other like products, the present invention from bifidobacterium longum BB, animal bifidobacteria V9,
Lactobacillus plantarum PP12, lactobacillus acidophilus NM screening, the optimization of medium optimization, condition of culture are started with, it is determined that optimal fermentation
Condition, discussion research is carried out, complete the industrialization production to probiotics preparation.From cell and Molecular level study protection agent prescription
Composition and the protection mechanism dried to each bacterium cell, the viable count of the product are of a relatively high.
In product development process, main technological difficulties are that there is excellent probiotics bacteria selection and product to match somebody with somebody
The collaboration compatibility research of several big strains in side.Animal bifidobacteria V9 is isolated from 12 healthy Mongols's paediatric faecals
11 plants of Bifidobacteriums in 1 plant of potential probiotics acidproof, bile tolerance characteristic is stronger screening.
The content of the invention
We use modern software analytical technology, respectively by clinical trial and zoopery to the prebiotic of compound probiotic
Function, biological characteristics have carried out system research, further with correlation theories knowledges such as clinical medicine, nutrition, statistics, lead to
Experimental verification and detection result indicator are crossed, works out scientific and reasonable compound probiotic formula.
To achieve the above object, the present invention provides a kind of compound probiotic powder preparation for strengthening immunity of pets, its feature
It is, it includes following compositions：
The present invention also provides a kind of application of compound probiotic powder preparation in the medicine for preparing enhancing immunity of pets.
The present invention also provides a kind of application of compound probiotic powder preparation in the medicine for preparing enhancing animal immunizing power.
The present invention also provides a kind of pet food or feed, and it includes composite bacterium powder preparation described above.
The present invention also provides a kind of animal foodstuff or feed, and it includes composite bacterium powder preparation described above.
Bifidobacterium longum BB, animal bifidobacteria V9, Lactobacillus plantarum PP12 and lactobacillus acidophilus NM in the present invention
With commercially available acquisition.
Brief description of the drawings
Below in conjunction with the accompanying drawings and embodiment the invention will be further described：
The experimental result that Fig. 1 composite probiotics preparations bacterial strain suppresses to stick to Escherichia coli；
The experimental result that Fig. 2 composite probiotics preparations bacterial strain suppresses to stick to salmonella；
The experimental result that Fig. 3 composite probiotics preparations bacterial strain suppresses to stick to Shigella.
Embodiment
In order to further elucidate rather than limit the present invention in more detail, the following example is provided.
The preparation of the composite probiotics preparations of embodiment 1.
Preparation process is as follows：
First, picking inclined-plane preserves bifidobacterium longum BB, animal bifidobacteria V9, Lactobacillus plantarum PP12, thermophilic respectively for activation
Lactobacillus lactis NM, it is inoculated into corresponding seed culture medium, is activated under suitable fermentation condition；
2nd, above strain activation and culture thing is cultivated with inoculum concentration：1ml culture mediums are taken to add in 1 seed bottle, fully dissolving
Afterwards, draw 0.5ml to add in 100ml primary seed solutions, 37 DEG C of 18~22h of aerobic culture.First order seed 1% (v/v) is inoculated in
Secondary seed solution, 37 DEG C of 18~22h of aerobic culture.Secondary seed 1% (v/v) is inoculated in three-level seed liquor, 37 DEG C of aerobic cultures,
It is fermentation termination during pH=4.35 ± 0.05.Three-level seed 7% (v/v) is inoculated in zymotic fluid, aerobic culture, constant pH 5.9.Hair
Fermentation tank pressure：Fermentation pressure inside the tank is to fill air 0.03M pa.Cultivation temperature：37℃.PH value controls：Constant pH 5.9.Neutralize
Agent：25% ammoniacal liquor.Mixing control：Mixing speed is 60~80 revs/min；
3rd, mixed fermentation strain is that above-mentioned four kinds of bacterium expand numerous pure culture by 1.0 × 1011cfu/ml、1.0×
1011cfu/ml、5.0×1011cfu/ml、5.0×109Cfu/ml, viable count, which is added, is equipped with auxiliary material progress composite bacteria agent.
The composite probiotics preparations adhesiveness test of embodiment 2.
Composite probiotics preparations are inoculated in fluid nutrient medium, 37 DEG C of culture 24h, activation is standby.By Tissue Culture Plate
With sterile PBS once, six holes are added to after lmL probiotic bacterial cultures are mixed with lmL RPMI-1640 cell culture fluids
In each hole of culture plate, 37 DEG C of CO are placed in21h is cultivated in incubator, cover glass is taken out, 5 is cleaned with sterile PBS cushioning liquid
Secondary, methanol fixes 10min, Gram's staining dyeing, and oily Microscopic observation adheres to situation.Cell is calculated according to bacterial adhesion index to glue
Attached amount.Table 1 is the adhesion data of compound probiotic.It is computed drawing：Cell adhesion index 105.
The compound probiotic of table 1. sticks index
The compound beneficial metabolite of embodiment 3. suppresses pathogen adhesion experiment
Pathogenic bacteria are inoculated in fluid nutrient medium, 37 DEG C of culture 24h, activated repeatedly standby after two generations.It is first that 1mL is prebiotic
Bacterium bacterium solution supernatant (three control groups) handles 1h after being mixed with 1mL pathogenic bacteria under the conditions of 37 DEG C, then by mixture and RPMI-
1640 cell culture fluids are added in each hole of six well culture plates, are placed in 37 DEG C of CO21h is cultivated in incubator.Afterwards with sterile PBS
Cleaning 5 times, add pancreatin and digested, once blow and beat cell with sterile PBS afterwards with sterile PBS, disengage it from six orifice plates.
PBS solution containing cell is crushed, then with plate count, the difference of contrast experiment's group and blank group.
Fig. 1, Fig. 2 and Fig. 3 are respectively that composite probiotics preparations bacterial strain suppresses to Escherichia coli, salmonella and Shigella
The experimental result sticked.
The Escherichia coli control group without any processing is counted as 7.34, MRS and adds lactic acid and hydrochloric acid as can be seen from Figure 1
6.86 and 6.81 are counted as, it sticks and is counted as 4.23 the probiotics handled by compound probiotic bacterium solution supernatant, than under control
Three orders of magnitude have dropped.
The bacterium number of sticking of untreated salmonella is 7.08 as can be seen from Figure 2, with the sand of the processing of lactic acid and hydrochloric acid
The bacterium number of sticking of door Salmonella is 6.76 and 6.56, and it is 4.28 that the salmonella handled by compound probiotic bacterium solution, which sticks bacterium number,
Reduce two orders of magnitude.
As can be seen from Figure 3 the control group bacterium number of Shigella is 6.54, with the Salmonella of the processing of lactic acid and hydrochloric acid
The bacterium number of sticking of bacterium is 5.87 and 5.72, and the Shigella bacterium number handled by compound probiotic bacterium solution supernatant is 4.11, under
Two orders of magnitude have dropped.
Probiotics will enter to be played a role, it is necessary to attach to first on intestinal epithelial cell in vivo, and definite value could be sent out afterwards
Bacteriostasis is waved, suppresses the generation of diarrhoea.From experimental data show compound probiotic bacterial strain stick index reach 100 with
On.From experimental result it can be seen from the figure that compared with the control group without probiotics bacterial liquid, probiotics bacterial liquid can greatly shadow
The growth of pathogenic bacteria is rung, the growth concentration of pathogenic bacteria is only capable of reaching 60% or so of control group concentration.Illustrate that probiotics suppresses to cause
Germ suppresses the growth of pathogenic bacteria mainly by its caused metabolite, while also indicates that thalline is made to the growth for suppressing pathogenic bacteria
With limited.It can be seen that this compound probiotic can reduce pathogenic bacteria well and be attached on intestinal cell from experimental result, and
Add the experimental group of lactic acid and hydrochloric acid and can not then reduce pathogenic bacteria and stick, illustrate simple by acid drop caused by probiotics metabolism
Low pH can not be reduced sticking for pathogenic bacteria, and moieties suppress sticking for pathogenic bacteria in metabolite.
Influence of the composite probiotics preparations of embodiment 3. to growth of animal performance and immunologic function
Probiotics can produce the enzyme of multiple beneficial after enteron aisle is entered, and promote digestion of the body to nutriment to inhale
Receive.Research shows that probiotics can increase the metabolism synthesis of body internal protein, vitamin, lipid, can by increasing feed intake
Promote growth and immune response and other effects to produce, can also improve antibody level.This is probably because probiotics is as a kind of anti-
Original can promote Development of Immune Organs, so that more lymphocyte differentiation plasmablasts produce antibody.And numerous studies
Also it has been proved that antibiotic easily develops immunity to drugs, fowl poultry immune function is suppressed.This way has been limited in Europe
System.As can be seen here, the economic benefit of animal husbandry can be significantly improved using probiotics micro-ecological formulation substitute antibiotics, moreover it is possible to avoid
The toxic side effect that medicine band is come, there is original economic results in society.Therefore based on the present invention chooses poultry chick, micro- life is utilized
State preparation feeding chickling, its influence to growth performance of chicks and immunity function is explored, so as to be probiotics compound microecological system
The pet that is applied to of agent provides theoretical foundation, promotes promoting the use of for composite probiotics preparations.
3.1 experimental method
An age in days Laying chicks are chosen, normal feeding observation 3 days, after eliminating diseased chicken and weak chicken, are chosen similar in 100 body weight
Healthy Laying chicks are randomly divided into a control group and an experimental group, every group 50 (often repeating 10, cage, totally 5 repetition cages).
Probiotics micro-ecological additive used in experiment is by bifidobacterium longum BB, animal bifidobacteria V9, Lactobacillus plantarum PP12, acidophilus breast
The compound micro-ecological preparation of bacillus NM compositions, laboratory self-control.Viable bacteria amount >=1.0 × 109CFU/g.All Laying chicks are taken certainly
By searching for food and drinking water, eye droppings and collunarium mode vaccine inoculation are taken in seven ages in days.Test period is 6 weeks, other raising operations
Carried out in strict accordance with Laying chicks (0-6 week old) feeding and management method.
Control group：Basal diet.
Test group：Basal diet+200mg/kg composite probiotics preparations.
3.1.1 influence of the probiotics compound micro-ecological preparation to growth performance of chicks
The different phase chick weight gains of table 2, feed consumption rate and feed-weight ratio (unit：g)
Same week old chick, in control group and test group, there were significant differences that (same letter represents that difference is not notable, and letter is not
With expression significant difference)；For test group compared with control group, experimental period, inner circumferential weightening had significant difference (p ＜ 0.05), gave
The test group weightening of composite probiotics preparations will be significantly higher than control group, and this result shows that young bird can be promoted by adding probiotics preparation
Chicken grows, and improves efficiency of feed utilization.
3.1.2 influence of the composite probiotics preparations to Immune Organs Index of Chicks
Immune Organs Index (the unit of table 3：mg.g-1)
As shown in Table 3, the spleen of test group chick, the bursa of farbricius and thymus index are both greater than control group, significant difference (p ＜
0.05), show that development of the composite probiotics preparations to animal spleen, the bursa of farbricius and thymus gland immune organ has facilitation.Chicken
Immune level influenceed by the developmental condition of immune organ, the increase of immune organ relative weight, illustrate the immune level of body
Also increase.It is probably that can synthesize immune organ in reproductive process in enteron aisle because of profitable strain in preparation to grow not
The benefit materials such as the vitamin that can lack, amino acid.
Present invention demonstrates that can substantially be put on weight after preparation prepared by chick administering transgenic room, during 6 week old, test group compares
According to a group height；And in terms of feed-weight ratio, it can substantially reduce feed-weight ratio, during 6 week old, test group is lower than control group, because probiotics
Chick intestinal microecology balance can be adjusted, improves absorption of the body to nutriment, utilization rate.By biochemical indicator,
The Immune Organs Index index and spleen index of test group chick, bursal index and thymus index are higher than control group respectively, illustrate micro- life
State preparation can increase body immunity.The probiotics compound micro-ecological preparation of laboratory preparation is understood by above experimental data
There is facilitation to the immunologic function of chicken.
Claims (4)
1. a kind of compound probiotic powder preparation for strengthening immunity of pets, it is characterised in that it includes following compositions：
A kind of 2. compound probiotic powder preparation answering in the medicine for preparing enhancing immunity of pets according to claim 1
With.
3. a kind of pet food or feed, it includes the composite bacterium powder preparation described in claim 1.
4. the preparation method of composite probiotics preparations in claim 1, it is characterised in that
(1) picking inclined-plane preserves bifidobacterium longum BB, animal bifidobacteria V9, Lactobacillus plantarum PP12, acidophilus breast bar respectively for activation
Bacterium NM, it is inoculated into corresponding seed culture medium, is activated under suitable fermentation condition；
(2) above strain activation and culture thing is cultivated with inoculum concentration：Take 1ml culture mediums to add in 1 seed bottle, fully after dissolving, inhale
0.5ml is taken to add in 100ml primary seed solutions, 37 DEG C of 18~22h of aerobic culture.First order seed 1% (v/v) is inoculated in two level kind
Sub- liquid, 37 DEG C of 18~22h of aerobic culture.Secondary seed 1% (v/v) is inoculated in three-level seed liquor, 37 DEG C of aerobic cultures, pH=
It is fermentation termination when 4.35 ± 0.05.Three-level seed 7% (v/v) is inoculated in zymotic fluid, aerobic culture, constant pH 5.9.Fermentation tank
Pressure：Fermentation pressure inside the tank is to fill air 0.03M pa.Cultivation temperature：37℃.PH value controls：Constant pH 5.9.Nertralizer：
25% ammoniacal liquor.Mixing control：Mixing speed is 60~80 revs/min；
(3) mixed fermentation strain is that above-mentioned four kinds of bacterium expand numerous pure culture by 1.0 × 1011cfu/ml、1.0×1011cfu/ml、
5.0×1011cfu/ml、5.0×109Cfu/ml, viable count, which is added, is equipped with auxiliary material progress composite bacteria agent.
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| CN111567696A (en) * | 2020-05-09 | 2020-08-25 | 上海耐威克宠物用品有限公司 | Functional beverage for pets and preparation method thereof |
| CN111838446A (en) * | 2020-08-14 | 2020-10-30 | 山东美斯宠物食品股份有限公司 | Pet complete food added with tetragenous fungi |
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| CN112175053A (en) * | 2020-09-29 | 2021-01-05 | 内蒙古普泽生物制品有限责任公司 | Bacteriocin produced by lactobacillus acidophilus NM, preparation method and application thereof |
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| CN113717884A (en) * | 2021-08-20 | 2021-11-30 | 内蒙古普泽生物制品有限责任公司 | Development and application of lactobacillus acidophilus NM for inhibiting alpha-glucosidase |
| CN114934004A (en) * | 2022-07-06 | 2022-08-23 | 广东中科有益生物科技有限公司 | Composite probiotic powder preparation for enhancing immunity of pets and preparation method thereof |
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