CN107866561B - Extract solution from aloe separates single size In4The method of Ni nanosphere - Google Patents
Extract solution from aloe separates single size In4The method of Ni nanosphere Download PDFInfo
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- CN107866561B CN107866561B CN201711290204.3A CN201711290204A CN107866561B CN 107866561 B CN107866561 B CN 107866561B CN 201711290204 A CN201711290204 A CN 201711290204A CN 107866561 B CN107866561 B CN 107866561B
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- aloe
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/14—Treatment of metallic powder
- B22F1/145—Chemical treatment, e.g. passivation or decarburisation
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Abstract
The present invention relates to extract solution from aloe to be centrifugated single size face-centered cubic In4The method of Ni nanosphere.Chinese aloe leaf: 1) successively clean → being shredded → smashing to pieces → and filters → be centrifuged, then Aspirate supernatant by this method step, obtains extract solution from aloe；2) by face-centered cubic In4Ni nanosphere ultrasonic disperse in ultrapure water, is made uniform dispersion liquid；3) extract solution from aloe of various concentration is prepared；3) extract solution from aloe that various concentration is sequentially added in centrifuge tube, is made into density gradient solution；4) by face-centered cubic In4Ni nanosphere dispersion liquid is added on extract solution from aloe density gradient solution and is centrifuged.Due to various sizes of face-centered cubic In4The rate of settling of the Ni nanosphere in extract solution from aloe density gradient solution is different, they will be trapped in the different location of density gradient solution, and extract solution from aloe can be obtained to the face-centered cubic In of single size with 40 DEG C of water elutions4Ni nanosphere, to achieve the effect that separation.The invention has the advantages that density gradient media solution green, environmental protection, good separating effect.
Description
Technical field
The present invention relates to a kind of extract solution from aloe to separate single size In4The method of Ni nanosphere belongs to advanced nanoparticle
Sub- separating technology technical field.
Background technique
Due to dimensional effect, nanoparticle has performance more superior than ordinary particle.As it can be seen that the nanoparticle that size is single
The acquisition of son has great meaning to applications to nanostructures and development.
At this stage, although there are many synthetic method for synthesizing the single mono-dispersed nano particle of size, these methods
General reactions condition requires harsher while at high cost.Density gradient centrifugation separation is that one kind is commonly used to large biological molecule
Separation method.Although people have developed the side that organic density gradient method separates different size of oil-soluble nano particles in recent years
Method, but the use of a large amount of organic solvents and repeatedly centrifuge separation, so that risk of environmental pollution is higher, larger workload.
Currently, the example for carrying out nanoparticle separation in extract solution from aloe is not yet seen in report.Use extract solution from aloe
The advantages of being separated is that working environment is friendly, green safe, and is 1 separation, greatly improves the efficiency, alleviates work
It measures.Simultaneously, it is only necessary to by extract solution from aloe with hot water elution, so that it may obtain the single mono-dispersed nano particle of size.It washes
It can be used repeatedly for the extract solution from aloe taken off.So extract solution from aloe is centrifugated single size In4The side of Ni nanosphere
Method, for further research single size In4The performance of Ni nanosphere provides basis, also will further illustrate function nano material
The dependence of size and structure enriches the Research foundation in the fields such as new catalyst, nano-device, is a meaningful work
Make, has great meaning to applications to nanostructures and development.
Summary of the invention
Single size In is separated using extract solution from aloe the object of the present invention is to provide a kind of4The method of Ni nanosphere.
In order to achieve the above objectives, the present invention adopts the following technical scheme:
Extract solution from aloe separates single size In4The method of Ni nanosphere, it is characterised in that the specific steps of this method are as follows:
A. Chinese aloe leaf is cleaned, shreds, selects top gear to smash to pieces in tissue mashing machine, smash liquid to pieces with double gauze and be filled into burning
In cup, filtrate moves into centrifuge tube, 4000 rpms on centrifuge, is centrifuged 20 minutes, gentle aspiration supernatant, as aloe
Extracting solution；
B. the In that will be prepared by chemical method4Ni nanosphere is configured to the nanometer that mass-volume concentration is 20 milligrams every milliliter
Ball disperses solution；
C. the successively extract solution from aloe density gradient solution of compound concentration different (i.e. density is descending), by from high concentration
Sequence to low concentration successively takes 6 milliliters of extract solution from aloe that centrifuge tube is added, and is configured to gradient solution；
D. a certain amount of step b acquired solution is placed on the resulting extract solution from aloe density gradient solution of step c；
E. be 25 DEG C in temperature, revolving speed is 40000 revs/min, and the time is to be centrifuged under conditions of 8 hours, obtain it is a kind of by
According to In4The dispersion liquid of Ni nanosphere certain size size distribution；
F. the nanoparticle dispersion liquid after centrifugation is taken out in batches by top；Finally obtain different single size sizes
In4Ni nanosphere.
G. In described in technical scheme steps b4The size range of Ni nanosphere is from 1 nanometer to 25 nanometer.
H. technical solution is 1 separation.
Compared with prior art, the present invention having the following advantages and outstanding effects: 1. extract solution from aloe density gradient is molten
Liquid is environmental-friendly, green safe；2. separative efficiency is high, the In for separating different single sizes can be completed for 1 time4Ni nanosphere；3. pure
Abbreviation list, it is only necessary to by extract solution from aloe with hot water elution, so that it may be readily attained monodispersed In4Ni nanosphere；4. eluting
Extract solution from aloe it can be used repeatedly.
Detailed description of the invention
Fig. 1 is In of the embodiment of the present invention4The optical photograph of Ni nanosphere corresponding centrifuge tube after isolation, 1 in Fig. 1,
2,3,4 refer respectively to In4The layer of extract solution from aloe density gradient solution is trapped in after the separation of Ni nanosphere.
Fig. 2 is the unsegregated In of the embodiment of the present invention4The transmission electron microscope photo of Ni nanosphere.
Fig. 3 is the In that the embodiment of the present invention corresponds to the 4th layer of centrifuge tube in Fig. 1 after isolation4The transmission electricity of Ni nanosphere
Mirror photo.
Fig. 4 is the In that the embodiment of the present invention corresponds to the 3rd layer of centrifuge tube in Fig. 1 after isolation4The transmission electricity of Ni nanosphere
Mirror photo.
Fig. 5 is the In that the embodiment of the present invention corresponds to the 2nd layer of centrifuge tube in Fig. 1 after isolation4The transmission electricity of Ni nanosphere
Mirror photo.
Fig. 6 is the In that the embodiment of the present invention corresponds to the 1st layer of centrifuge tube in Fig. 1 after isolation4The transmission electricity of Ni nanosphere
Mirror photo.
Specific embodiment
Examples of implementation:
(1) firstly, preparing 20 milligrams per liter of In4Ni nanosphere dispersion liquid.
(2) it makes density gradient: being configured to mass percent 60%, 40%, 20%, 10% with extract solution from aloe and water
Extract solution from aloe；Since 60%, successively takes 6 milliliters of solution that centrifuge tube is gently added, be configured to gradient solution.
(3) by 2 milliliters of In4The dispersion liquid of Ni nanosphere is added on gradient liquid.
(4) at 25 DEG C, with 40000 rpms, above-mentioned liquid to be separated is centrifuged 8 hours.
(5) after being centrifuged, In4Ni nanosphere forms 4 layers in density gradient, 10% extract solution from aloe solution and 20% reed
The In separated between luxuriant growth extracting solution interface4The partial size of Ni nanosphere is 5 ± 1.5 nanometers, 20% extract solution from aloe and 40% aloe
The In separated between extracting solution interface4The partial size of Ni nanosphere is 9 ± 2.0 nanometers, and 40% extract solution from aloe is mentioned with 60% aloe
Take the In separated between liquid interface4The partial size of Ni nanosphere is 14 ± 3.0 nanometers, the In of 60% extract solution from aloe bottom end separation4Ni
The partial size of nanosphere is 20 ± 3.0 nanometers.Separating resulting is referring to Fig. 1-6.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. extract solution from aloe separates single size In4The method of Ni nanosphere, it is characterised in that this method carries out as follows:
A. Chinese aloe leaf is cleaned, shreds, selects top gear to smash to pieces in tissue mashing machine, smash liquid to pieces with double gauze and be filled into beaker
In, filtrate moves into centrifuge tube, 4000 rpms on centrifuge, is centrifuged 20 minutes, Aspirate supernatant, as aloe extract
Liquid；
B. by In4Ni nanosphere carries out ultrasonic disperse in ultrapure water, is configured to mass-volume concentration and is 20 milligrams every milliliter to receive
The rice uniform dispersion liquid of ball；
C. the resulting extract solution from aloe of step a is diluted with ultrapure water, needed for successively being taken by the sequence from high concentration to low concentration
Centrifuge tube is added in solution, is configured to gradient solution；
D. by In obtained by step b4Ni nanosphere dispersion liquid is placed on the resulting density gradient solution of step c；
E. it is 25 DEG C in temperature, the resulting mixture of step d is centrifuged under the conditions of revolving speed is 40000 rpms with centrifuge
8 hours, one kind was obtained according to In4The dispersion liquid of Ni nanosphere size distribution；
F. by the resulting In of step e4Ni nanosphere dispersion liquid takes out in batches, obtains various sizes of In4Ni nanosphere dispersion liquid group
Point.
2. extract solution from aloe according to claim 1 separates single size In4The method of Ni nanosphere, it is characterised in that: step
In described in rapid b4The size range of Ni nanosphere is from 1 nanometer to 25 nanometer.
3. extract solution from aloe according to claim 1 separates single size In4The method of Ni nanosphere, it is characterised in that: step
Mass percent concentration in rapid c for the extract solution from aloe of configuration density gradient solution is respectively 60%, 40%, 20%,
10%.
4. extract solution from aloe according to claim 1 separates single size In4The method of Ni nanosphere, it is characterised in that: step
For separating single size In in rapid c4The density gradient of the extract solution from aloe of Ni nanosphere is 4, and each gradient volume is 6 millis
It rises.
5. extract solution from aloe according to claim 1 separates single size In4The method of Ni nanosphere, it is characterised in that: reed
Luxuriant growth extracting solution is centrifugated single size In4The process of Ni nanosphere is 1 completion.
6. extract solution from aloe according to claim 1 separates single size In4The method of Ni nanosphere, after centrifugation, In4Ni
Nanosphere forms 4 layers in density gradient, separates between 10% extract solution from aloe solution and 20% extract solution from aloe interface
In4The partial size of Ni nanosphere is 5 ± 1.5 nanometers, is separated between 20% extract solution from aloe and 40% extract solution from aloe interface
In4The partial size of Ni nanosphere is 9 ± 2.0 nanometers, is separated between 40% extract solution from aloe and 60% extract solution from aloe interface
In4The partial size of Ni nanosphere is 14 ± 3.0 nanometers, the In of 60% extract solution from aloe bottom end separation4The partial size of Ni nanosphere be 20 ±
3.0 nanometer.
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| CN1580267A (en) * | 2003-08-14 | 2005-02-16 | 中国农业大学 | Magneto tactic bacteria nano magnetic particle extracting and purifying method |
| CN101559401A (en) * | 2009-05-26 | 2009-10-21 | 北京化工大学 | Method for separating nano-particles at water-phase density gradient centrifugation rate |
| JP2012153600A (en) * | 2007-03-27 | 2012-08-16 | National Institute Of Advanced Industrial Science & Technology | Method for directly separating metallic cnt and semiconductor cnt using centrifuge |
| CN102671589A (en) * | 2012-05-21 | 2012-09-19 | 北京化工大学 | Density gradient centrifugation method for analyzing nano grain surface reaction process |
| CN103723678A (en) * | 2013-12-23 | 2014-04-16 | 北京化工大学 | Method for switching media for nano-particles or separating same from one another according to sizes and/or shapes of nano-particles |
| CN104841926A (en) * | 2015-04-24 | 2015-08-19 | 天津宝兴威科技有限公司 | Nanometer silver wire length separation method |
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2017
- 2017-12-08 CN CN201711290204.3A patent/CN107866561B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580267A (en) * | 2003-08-14 | 2005-02-16 | 中国农业大学 | Magneto tactic bacteria nano magnetic particle extracting and purifying method |
| JP2012153600A (en) * | 2007-03-27 | 2012-08-16 | National Institute Of Advanced Industrial Science & Technology | Method for directly separating metallic cnt and semiconductor cnt using centrifuge |
| CN101559401A (en) * | 2009-05-26 | 2009-10-21 | 北京化工大学 | Method for separating nano-particles at water-phase density gradient centrifugation rate |
| CN102671589A (en) * | 2012-05-21 | 2012-09-19 | 北京化工大学 | Density gradient centrifugation method for analyzing nano grain surface reaction process |
| CN103723678A (en) * | 2013-12-23 | 2014-04-16 | 北京化工大学 | Method for switching media for nano-particles or separating same from one another according to sizes and/or shapes of nano-particles |
| CN104841926A (en) * | 2015-04-24 | 2015-08-19 | 天津宝兴威科技有限公司 | Nanometer silver wire length separation method |
Non-Patent Citations (2)
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| 纳米材料的密度梯度离心分离的研究进展(上);李鹏松 等;《石油化工》;20160615;第45卷(第6期);648-655,670 |
| 纳米材料的密度梯度离心分离的研究进展(下);李鹏松 等;《石油化工》;20160715;第45卷(第7期);776-782 |
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