CN107865444A - A kind of preparation method of nanogel - Google Patents
A kind of preparation method of nanogel Download PDFInfo
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- CN107865444A CN107865444A CN201711228685.5A CN201711228685A CN107865444A CN 107865444 A CN107865444 A CN 107865444A CN 201711228685 A CN201711228685 A CN 201711228685A CN 107865444 A CN107865444 A CN 107865444A
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- nanogel
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- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 229920001503 Glucan Polymers 0.000 claims abstract description 10
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 10
- 239000004455 soybean meal Substances 0.000 claims abstract description 10
- 244000068988 Glycine max Species 0.000 claims abstract description 6
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 6
- 235000013601 eggs Nutrition 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 108700037728 Glycine max beta-conglycinin Proteins 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 238000002242 deionisation method Methods 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 10
- 239000002245 particle Substances 0.000 abstract description 5
- 238000004220 aggregation Methods 0.000 abstract description 4
- 230000002776 aggregation Effects 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 description 6
- 239000005017 polysaccharide Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000001338 self-assembly Methods 0.000 description 5
- 108010060630 Lactoglobulins Proteins 0.000 description 3
- 102000008192 Lactoglobulins Human genes 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108010073771 Soybean Proteins Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 235000019710 soybean protein Nutrition 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- HEFNNWSXXWATRW-UHFFFAOYSA-N Ibuprofen Chemical compound CC(C)CC1=CC=C(C(C)C(O)=O)C=C1 HEFNNWSXXWATRW-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000012296 anti-solvent Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 239000011258 core-shell material Substances 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Beans For Foods Or Fodder (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of preparation method of nanogel, and it includes following preparation process：Take following raw material for standby：Defatted soybean meal, glucan, ANS；Soybean 7S ball eggs are isolated from defatted soybean meal；Nanogel prepared by the method for the invention, has high stability, does not have Second Aggregation in the range of pH 2~12, its particle diameter is basically unchanged；It is little that 13 months change of size are deposited at 4 DEG C；Lyophilized redissolve does not influence substantially on the particle diameter of nanogel.Have a good application prospect.
Description
Technical field
The present invention relates to a kind of preparation method of nanogel.
Background technology
In recent years, nanosecond science and technology become most rich vigor and most potential emerging technology, and nano material is due to it
Unique skin effect and small-size effect etc. have obtained extensive concern.The method for building nano material divides method " from top to bottom "
" from bottom to up " method.Self assembly refers to that elementary cell spontaneously becomes to have from disordered state as a kind of " from bottom to up " method
The method of sequence aggregation.2009, Schmitt etc. was prepared with 1% beta lactoglobulin in the range of pH3~7 by self-assembly method
The micro- glue of beta lactoglobulin.Research finds that beta lactoglobulin can form linear solubility under conditions of pH ＜ 4.8 or pH ＞ 5.6
Aggregation, and pH4.8~5.6 can form spherical micro- glue with colloidal stability in isoelectric point scope.2014, Chen etc.
The soybean protein nanogel with certain stability is prepared in 95 DEG C of heating under conditions of pH5.9 with soybean protein, but should
Nanogel has pH sensitiveness.
Numerous studies show that amphipathic nature block polymer can be self-assembly of the stable nanoparticle with core shell structure
Son.Albumen and polysaccharide are two major class natural macromolecular materials in food system, can make egg by spontaneous Maillard reactions
Whiteε- orαCovalent bond occurs for the carbonyl of-amino and reduced sugar, obtains that there is the protein-polysaccharide of superior functionality characteristic to be total to
Valency compound.Therefore, amphipathic nature block polymer is prepared by Maillard reactions and further self assembly can obtains egg
White matter-polysaccharide nanogel.Wu etc. is prepared spherical using the product and its hydrolysate of Maillard reactions by anti-solvent method
Nano-particle, but glutaraldehyde used-ethanol crosslinking agent has certain toxicity, is unfavorable for preparing environmentally friendly nanometer material
Material.The research and utilization such as Li Maillard reacts and heated gel method prepares lysozyme-glucan that hydrated diameter is about 200nm and received
Rice gel, and brufen is loaded.Research shows that the nanogel has higher stability, in the range of certain pH
Do not assemble or dissociate.However, research shows, when the size of particle is down to nanoscale, be advantageous to improve it in vivo
Slow release effect, increase its circulation time in vivo.Soybean 7 S globulin is albumen main in soybean, and having, which reduces courage, consolidates
Alcohol, reducing blood lipid etc. improve the function of fat metabolism.Both at home and abroad on the reaction of the covalent bond of soybean 7 S globulin and polysaccharide
There is the research report of correlation, be further discovered that soybean 7 S globulin can be with after being combined by polysaccharide covalent on this Research foundation
Further assembling forms nanogel well, and nano-particle is built using the self assembly of proteoglycan compound, is a kind of safety
Nontoxic, green processing mode.Protein-polysaccharide covalent compound nanogel is prepared with Maillard reactions at present
Research is also not directed to substantially.
The content of the invention
The present invention proposes a kind of preparation method of nanogel.
A kind of preparation method of nanogel of the present invention, it includes following preparation process：
（1）Take following raw material for standby：Defatted soybean meal, glucan, ANS；
（2）Soybean 7S ball eggs are isolated from defatted soybean meal；
（3）It is 1 to take mass ratio respectively:1 soybean 7 S globulin and glucan mixing, mixture, which is dissolved in deionized water, to be made
Its concentration respectively reaches 100g/L, is sufficiently stirred 4-8h, adjusts pH to 7-9, and be freeze-dried；
（4）Dried powder crosses the screen cloth that screen size is 0.125mm, is then placed within bottom and saturation KBr solution is housed
In drier, 4d is reacted in 60 DEG C；
（5）Products therefrom presses mass volume ratio 1:10 ratio adds deionized water, and stirring certain time makes it fully dissolve；
（6）10000gAfter centrifuging 15min, supernatant is freeze-dried, and gained powder is SDC, is placed in drier and preserves
It is standby；
（7）Take a certain amount of SDC powder to be dissolved in the uniform solution for being made into that concentration is 1mg/ml in a certain amount of deionization, use
0.1L HCl solution regulation pH to 3-5, is then placed within 80-100 DEG C of water-bath and is incubated 30-60min, be after cooling
SDN。
Preferably, the step（4）The relative humidity of middle drier is 79%.
Preferably, the step（2）PH value be 7.
Preferably, the step（7）PH value be 4.8.
Preferably, the step（7）Bath temperature be 95 DEG C, water bath time 50min.
Nanogel prepared by the method for the invention, has high stability, not secondary in the range of pH 2~12
Aggregation, its particle diameter are basically unchanged；It is little that 13 months change of size are deposited at 4 DEG C；The lyophilized particle diameter base redissolved to nanogel
This does not influence.Have a good application prospect.
Embodiment
Embodiment 1.
A kind of preparation method of nanogel of the present invention, it includes following preparation process：
（1）Take following raw material for standby：Defatted soybean meal, glucan, ANS；
（2）Soybean 7S ball eggs are isolated from defatted soybean meal；
（3）It is 1 to take mass ratio respectively:1 soybean 7 S globulin and glucan mixing, mixture, which is dissolved in deionized water, to be made
Its concentration respectively reaches 100g/L, is sufficiently stirred 4-8h, adjusts pH to 7-9, and be freeze-dried；
（4）Dried powder crosses the screen cloth that screen size is 0.125mm, is then placed within bottom and saturation KBr solution is housed
In drier, 4d is reacted in 60 DEG C；
（5）Products therefrom presses mass volume ratio 1:10 ratio adds deionized water, and stirring certain time makes it fully dissolve；
（6）10000gAfter centrifuging 15min, supernatant is freeze-dried, and gained powder is SDC, is placed in drier and preserves
It is standby；
（7）Take a certain amount of SDC powder to be dissolved in the uniform solution for being made into that concentration is 1mg/ml in a certain amount of deionization, use
0.1L HCl solution regulation pH to 3-5, is then placed within 80-100 DEG C of water-bath and is incubated 30-60min, be after cooling
SDN。
Step described in the present embodiment（4）The relative humidity of middle drier is 79%；The step（2）PH value be 7.
Embodiment 2.
A kind of preparation method of nanogel of the present invention, it includes following preparation process：
（1）Take following raw material for standby：Defatted soybean meal, glucan, ANS；
（2）Soybean 7S ball eggs are isolated from defatted soybean meal；
（3）It is 1 to take mass ratio respectively:1 soybean 7 S globulin and glucan mixing, mixture, which is dissolved in deionized water, to be made
Its concentration respectively reaches 100g/L, is sufficiently stirred 4-8h, adjusts pH to 7-9, and be freeze-dried；
（4）Dried powder crosses the screen cloth that screen size is 0.125mm, is then placed within bottom and saturation KBr solution is housed
In drier, 4d is reacted in 60 DEG C；
（5）Products therefrom presses mass volume ratio 1:10 ratio adds deionized water, and stirring certain time makes it fully dissolve；
（6）10000gAfter centrifuging 15min, supernatant is freeze-dried, and gained powder is SDC, is placed in drier and preserves
It is standby；
（7）Take a certain amount of SDC powder to be dissolved in the uniform solution for being made into that concentration is 1mg/ml in a certain amount of deionization, use
0.1L HCl solution regulation pH to 3-5, is then placed within 80-100 DEG C of water-bath and is incubated 30-60min, be after cooling
SDN。
Step described in the present embodiment（4）The relative humidity of middle drier is 79%；The step（2）PH value be 7；It is described
Step（7）PH value be 4.8；The step（7）Bath temperature be 95 DEG C, water bath time 50min.
Claims (5)
1. a kind of preparation method of nanogel, it is characterised in that including following preparation process：
（1）Take following raw material for standby：Defatted soybean meal, glucan, ANS；
（2）Soybean 7S ball eggs are isolated from defatted soybean meal；
（3）It is 1 to take mass ratio respectively:1 soybean 7 S globulin and glucan mixing, mixture, which is dissolved in deionized water, to be made
Its concentration respectively reaches 100g/L, is sufficiently stirred 4-8h, adjusts pH to 7-9, and be freeze-dried；
（4）Dried powder crosses the screen cloth that screen size is 0.125mm, is then placed within bottom and saturation KBr solution is housed
In drier, 4d is reacted in 60 DEG C；
（5）Products therefrom presses mass volume ratio 1:10 ratio adds deionized water, and stirring certain time makes it fully dissolve；
（6）10000gAfter centrifuging 15min, supernatant is freeze-dried, and gained powder is SDC, is placed in drier and preserves
It is standby；
（7）Take a certain amount of SDC powder to be dissolved in the uniform solution for being made into that concentration is 1mg/ml in a certain amount of deionization, use
0.1L HCl solution regulation pH to 3-5, is then placed within 80-100 DEG C of water-bath and is incubated 30-60min, be after cooling
SDN。
A kind of 2. preparation method of nanogel as claimed in claim 1, it is characterised in that the step（4）The phase of middle drier
It is 79% to humidity.
A kind of 3. preparation method of nanogel as claimed in claim 1, it is characterised in that the step（2）PH value be 7.
A kind of 4. preparation method of nanogel as claimed in claim 1, it is characterised in that the step（7）PH value be 4.8.
A kind of 5. preparation method of nanogel as claimed in claim 1, it is characterised in that the step（7）Bath temperature be
95 DEG C, water bath time 50min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711228685.5A CN107865444A (en) | 2017-11-29 | 2017-11-29 | A kind of preparation method of nanogel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711228685.5A CN107865444A (en) | 2017-11-29 | 2017-11-29 | A kind of preparation method of nanogel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107865444A true CN107865444A (en) | 2018-04-03 |
Family
ID=61754917
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711228685.5A Withdrawn CN107865444A (en) | 2017-11-29 | 2017-11-29 | A kind of preparation method of nanogel |
Country Status (1)
| Country | Link |
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| CN (1) | CN107865444A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114948863A (en) * | 2022-06-15 | 2022-08-30 | 四川大学 | Medicine for treating atherosclerosis |
-
2017
- 2017-11-29 CN CN201711228685.5A patent/CN107865444A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114948863A (en) * | 2022-06-15 | 2022-08-30 | 四川大学 | Medicine for treating atherosclerosis |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
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Application publication date: 20180403 |