CN107864853A - Brocade flower fast breeding method is changed based on tissue cultures - Google Patents
Brocade flower fast breeding method is changed based on tissue cultures Download PDFInfo
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- CN107864853A CN107864853A CN201610854248.3A CN201610854248A CN107864853A CN 107864853 A CN107864853 A CN 107864853A CN 201610854248 A CN201610854248 A CN 201610854248A CN 107864853 A CN107864853 A CN 107864853A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
Brocade flower fast breeding method is changed based on tissue cultures the present invention relates to a kind of, is comprised the following steps：(1) bead inducing culture, adventitious bud proliferation culture medium, strong seedling culture base, root media are prepared, (2) change the tissue cultures of brocade flower.Compared with prior art, the present invention is by tissue culture technique, year breeding coefficient bring up to 1:100000, cost lowers 0.3 0.5 yuan/plant, and because plant bulbs are embedded in soil, easily pollution, tissue culture difficulty is larger, changes the regularity that reproduction speed and seedling is greatly improved in brocade flower tissue-cultured seedling, preferably keeps original maternal character.
Description
Technical field
The present invention relates to a kind of plant breeding methods, and brocade flower fast breeding is changed based on tissue cultures more particularly, to a kind of
Method.
Background technology
It is Amaryllidaceae lycoris plants to change bright and beautiful flower, and draft, ground is given birth to.Bulb is avette, and early spring goes out leaf, leaf banding.Scape is high about
60 centimetres；2 pieces of phyllary.Umbel, there is colored 4-6, lilac red.The month at florescence 8-9.It is easy to change brocade flower cultivation, in nature
Under state, change brocade flower and set seeds few, and germination percentage is extremely low, so typically with asexual reproduction method expand numerous.More group planting in gardens
Or under flower border, roadside plant, the woods.Change brocade and spend resistance to half shade, sparse woods cover plant can be done again, be good presbyopic glasses and garden material
Material.As the tolerant plant kind of summer-flowering, its bulb separation is slow, the huge market demand, and seedling supply is restricted.
Chinese patent CN103503647A discloses a kind of method for changing brocade flower florescence control, can be used as change bright and beautiful flowerpot plant or
The supporting technology of the functional developments such as cut-flower application and scientific research are used.Specific steps include：(1) bright and beautiful seeds of flowering plants ball storage, is changed
Time；(2) condition of bright and beautiful seeds of flowering plants ball storage, is changed；(3) mode of bright and beautiful seeds of flowering plants ball storage, is changed；(4), change brocade flower and urge the time spent；
(5) method for, changing brocade flower flower forcing.Carry out changing the florescence control of brocade flower by the way of deepfreeze high temperature flower forcing, it is not necessary to apply
With any growth regulator, method is simple for it, is not influenceed by external environmental condition not only；Also can use Cold storage in the refrigerator and
The mode that greenhouse flower forcing is combined carries out substantial amounts of production practices.Brocade the flowers are in blossom the flowering plant that changes obtained can be used for potted plant viewing and admiring or cutting
Flower.The method for changing brocade flower florescence control is which disclose, but does not provide the correlation technique how bred and change brocade flower.
Chinese patent CN103734019A discloses a kind of method for tissue culture of New Zealand flax, and this method bag is prepared different
The culture medium in stage carries out tissue cultures, can improve the regularity of reproduction speed and seedling, preferably keeps original female parent
The advantages that character.But due to New Zealand flax with change brocade flower habit and required environment difference of breeding it is larger, above-mentioned tissue cultures
Method cannot be directly used to change the reproductive process of brocade flower.
The content of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide one kind breed speed it is fast, into
Motility rate is high to change brocade flower fast breeding method based on tissue cultures.
The purpose of the present invention can be achieved through the following technical solutions：
It is a kind of to change brocade flower fast breeding method based on tissue cultures, comprise the following steps：
(1) preparation of culture medium, the component and content of each stage culture medium of tissue culture are included：
1.1st, bead inducing culture：MS+BA 1-5mg/L+NAA 0.1-0.5mg/L；
1.2nd, adventitious bud proliferation culture medium：MS+6-BA 1.0-3.0mg/L+NAA 0.1-0.3mg/L；
1.3rd, strong seedling culture base：MS+6-BA 0.5-1.5mg/L+NAA 0.05-0.15mg/L；
1.4th, root media：MS+NAA 0.1-0.3mg/L；
The pH5.8 of above-mentioned various culture mediums, sucrose 30g/L, agar 6g/L are added, cultivation temperature is 25 ± 1 DEG C, and illumination is
1400-1600Lx；
(2) tissue cultures of brocade flower are changed：
2.1st, the acquisition of sterilizable material
The bead that brocade flower takes aerial part the 10-11 months in the fall is changed, peels off the bulb of outside with after running water flushing 1h
In on superclean bench, 30s, 1 ‰ mercuric chloride immersion 15min, aseptic water washing 5-6 times, aseptic filter paper are soaked with 75% ethanol
Surface moisture content is blotted, takes bead base portion to be cut into 1cm length, is inoculated on bead inducing culture；
2.2nd, the differentiation of bud and propagation
Bead is inoculated on inducing culture after 30 days, and bead base portion starts to expand, and yellow green projection is occurred, is further cultured for 25
Its visible obvious callus, continue culture 30 days, cut dribbling callus and be put on adventitious bud proliferation culture medium and cultivate；
2.3rd, adventitious bud strong seedling culture
On adventitious bud proliferation culture medium, the only 2-3 beads of Multiple Buds every clump induced can extend, and remaining is in short
Change state, after being divided into little Cong or single balls, strong seedling culture base is put into, adventitious bud is extended rapidly, and 2-3cm can be grown to after 20 days；
2.4th, culture of rootage
1-2cm plantlet is taken, root induction in root media of transferring, seedling base portion differentiates many white after 20 days
The root restriction of color, it can be grown to 1-2cm after 30 days；
2.5, hardening and transplanting
After culture of rootage 28-32 days, when root system length is to 1-2cm, the aseptic seedling of well developed root system robust growth is selected, interior is opened
Bottle hardening 3 days, cleans root agar after taking-up, hardening is tamed in greenhouse after 40 days, i.e. transplanting is outdoor, gives rich water quality management,
Transplanting survival rate is more than 95%.
Preferably, the component and content of described bead inducing culture are：MS+BA 3mg/L+NAA 0.3mg/L,
Bead growing way is best under the culture medium.
Preferably, the component and content of described adventitious bud proliferation culture medium are：MS+6-BA 2.0mg/L+NAA
0.2mg/L。
Preferably, the component and content of described strong seedling culture base are：MS+6-BA 1.0mg/L+NAA 0.1mg/L.
Preferably, the component and content of described root media are：MS+NAA 0.2mg/L, under the culture medium, root
It is sturdy, fibrous root is numerous, rooting rate 100%.
It is very big especially for the requirement difference of culture medium because different plants require different for condition of culture, wherein
The change of the culture medium main component or content of one link is possible to cause to breed substantially reducing for success rate.Therefore, originally
Invention passes through permanent experimentation, and according to the endemic plant growth chracteristic and growth demand for changing brocade flower, have studied and be adapted to change
A variety of culture mediums of brocade flower fast breeding, on the medium base using these special developments, with reference to the culture bar of the present invention
Part, successful incubation change bright and beautiful flower.
The present invention by tissue culture technique, year breeding coefficient bring up to 1:100000, cost lowers 0.3-0.5 members/strain,
Because plant bulbs are embedded in soil, easily pollution, tissue culture difficulty is larger, changes brocade flower tissue-cultured seedling and the whole of reproduction speed and seedling is greatly improved
Qi Du, preferably keep original maternal character.
Embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
It is a kind of to change brocade flower fast breeding method based on tissue cultures, comprise the following steps：
(1) preparation of culture medium, the component and content of each stage culture medium of tissue culture are included：
1.1st, bead inducing culture：MS+BA 3mg/L+NAA 0.3mg/L；
1.2nd, adventitious bud proliferation culture medium：MS+6-BA 2.0mg/L+NAA 0.2mg/L；
1.3rd, strong seedling culture base：MS+6-BA 1.0mg/L+NAA 0.1mg/L；
1.4th, root media：MS+NAA 0.2mg/L；
The pH5.8 of above-mentioned various culture mediums, sucrose 30g/L, agar 6g/L are added, cultivation temperature is 25 DEG C, and illumination is
1500Lx；
(2) tissue cultures of brocade flower are changed：
2.1st, the acquisition of sterilizable material
The bead that brocade flower takes aerial part the 10-11 months in the fall is changed, peels off the bulb of outside with after running water flushing 1h
In on superclean bench, 30s, 1 ‰ mercuric chloride immersion 15min, aseptic water washing 5-6 times, aseptic filter paper are soaked with 75% ethanol
Surface moisture content is blotted, takes bead base portion to be cut into 1cm length, is inoculated on bead inducing culture；
2.2nd, the differentiation of bud and propagation
Bead is inoculated on inducing culture after 30 days, and bead base portion starts to expand, and yellow green projection is occurred, is further cultured for 25
Its visible obvious callus, continue culture 30 days, cut dribbling callus and be put on adventitious bud proliferation culture medium and cultivate；
2.3rd, adventitious bud strong seedling culture
On adventitious bud proliferation culture medium, the only 2-3 beads of Multiple Buds every clump induced can extend, and remaining is in short
Change state, after being divided into little Cong or single balls, strong seedling culture base is put into, adventitious bud is extended rapidly, and 2-3cm can be grown to after 20 days；
2.4th, culture of rootage
1-2cm plantlet is taken, root induction in root media of transferring, seedling base portion differentiates many white after 20 days
The root restriction of color, it can be grown to 1-2cm after 30 days；
2.5th, hardening and transplanting
After culture of rootage 30 days, when root system length is to 1.5cm, the aseptic seedling of well developed root system robust growth, indoor corkage are selected
Hardening 3 days, root agar is cleaned after taking-up, hardening is tamed in greenhouse after 40 days, be i.e. transplanting is outdoor, gives rich water quality management, moves
It is 98% to plant survival rate.
Embodiment 2
It is a kind of to change brocade flower fast breeding method based on tissue cultures, comprise the following steps：
(1) preparation of culture medium, the component and content of each stage culture medium of tissue culture are included：
1.1st, bead inducing culture：MS+BA 1mg/L+NAA 0.1mg/L；
1.2nd, adventitious bud proliferation culture medium：MS+6-BA 1.0mg/L+NAA 0.1mg/L；
1.3rd, strong seedling culture base：MS+6-BA 0.5mg/L+NAA 0.05mg/L；
1.4th, root media：MS+NAA 0.1mg/L；
The pH5.8 of above-mentioned various culture mediums, sucrose 30g/L, agar 6g/L are added, cultivation temperature is 24 DEG C, and illumination is
1400Lx；
(2) tissue cultures of brocade flower are changed：
2.1, the acquisition of sterilizable material
The bead that brocade flower takes aerial part the 10-11 months in the fall is changed, peels off the bulb of outside with after running water flushing 1h
In on superclean bench, 30s, 1 ‰ mercuric chloride immersion 15min, aseptic water washing 5-6 times, aseptic filter paper are soaked with 75% ethanol
Surface moisture content is blotted, takes bead base portion to be cut into 1cm length, is inoculated on bead inducing culture；
2.2, the differentiation of bud and propagation
Bead is inoculated on inducing culture after 30 days, and bead base portion starts to expand, and yellow green projection is occurred, is further cultured for 25
Its visible obvious callus, continue culture 30 days, cut dribbling callus and be put on adventitious bud proliferation culture medium and cultivate；
2.3, adventitious bud strong seedling culture
On adventitious bud proliferation culture medium, the only 2-3 beads of Multiple Buds every clump induced can extend, and remaining is in short
Change state, after being divided into little Cong or single balls, strong seedling culture base is put into, adventitious bud is extended rapidly, and 2-3cm can be grown to after 20 days；
2.4th, culture of rootage
1-2cm plantlet is taken, root induction in root media of transferring, seedling base portion differentiates many white after 20 days
The root restriction of color, it can be grown to 1-2cm after 30 days；
2.5th, hardening and transplanting
After culture of rootage 28 days, when root system length is to 1cm, the aseptic seedling of well developed root system robust growth, indoor corkage refining are selected
Seedling 3 days, root agar is cleaned after taking-up, hardening is tamed in greenhouse after 40 days, be i.e. transplanting is outdoor, gives rich water quality management, transplants
Survival rate is 95%.
Embodiment 3
It is a kind of to change brocade flower fast breeding method based on tissue cultures, comprise the following steps：
(1) preparation of culture medium, the component and content of each stage culture medium of tissue culture are included：
1.1st, bead inducing culture：MS+BA 5mg/L+NAA 0.5mg/L；
1.2nd, adventitious bud proliferation culture medium：MS+6-BA 3.0mg/L+NAA 0.3mg/L；
1.3rd, strong seedling culture base：MS+6-BA 1.5mg/L+NAA 0.15mg/L；
1.4th, root media：MS+NAA 0.3mg/L；
The pH5.8 of above-mentioned various culture mediums, sucrose 30g/L, agar 6g/L are added, cultivation temperature is 26 DEG C, and illumination is
1600Lx；
(2) tissue cultures of brocade flower are changed：
2.1st, the acquisition of sterilizable material
The bead that brocade flower takes aerial part the 10-11 months in the fall is changed, peels off the bulb of outside with after running water flushing 1h
In on superclean bench, 30s, 1 ‰ mercuric chloride immersion 15min, aseptic water washing 5-6 times, aseptic filter paper are soaked with 75% ethanol
Surface moisture content is blotted, takes bead base portion to be cut into 1cm length, is inoculated on bead inducing culture；
2.2nd, the differentiation of bud and propagation
Bead is inoculated on inducing culture after 30 days, and bead base portion starts to expand, and yellow green projection is occurred, is further cultured for 25
Its visible obvious callus, continue culture 30 days, cut dribbling callus and be put on adventitious bud proliferation culture medium and cultivate；
2.3rd, adventitious bud strong seedling culture
On adventitious bud proliferation culture medium, the only 2-3 beads of Multiple Buds every clump induced can extend, and remaining is in short
Change state, after being divided into little Cong or single balls, strong seedling culture base is put into, adventitious bud is extended rapidly, and 2-3cm can be grown to after 20 days；
2.4th, culture of rootage
1-2cm plantlet is taken, root induction in root media of transferring, seedling base portion differentiates many white after 20 days
The root restriction of color, it can be grown to 1-2cm after 30 days；
2.5th, hardening and transplanting
After culture of rootage 32 days, when root system length is to 2cm, the aseptic seedling of well developed root system robust growth, indoor corkage refining are selected
Seedling 3 days, root agar is cleaned after taking-up, hardening is tamed in greenhouse after 40 days, be i.e. transplanting is outdoor, gives rich water quality management, transplants
Survival rate is 96%.
The above-mentioned description to embodiment is understood that for ease of those skilled in the art and using invention.
Person skilled in the art obviously can easily make various modifications to these embodiments, and described herein general
Principle is applied in other embodiment without by performing creative labour.Therefore, the invention is not restricted to above-described embodiment, ability
Field technique personnel do not depart from improvement that scope made and modification all should be the present invention's according to the announcement of the present invention
Within protection domain.
Claims (5)
1. a kind of change brocade flower fast breeding method based on tissue cultures, it is characterised in that comprises the following steps：
(1) preparation of culture medium, the component and content of each stage culture medium of tissue culture are included：
1.1st, bead inducing culture：MS+BA 1-5mg/L+NAA 0.1-0.5mg/L；
1.2nd, adventitious bud proliferation culture medium：MS+6-BA 1.0-3.0mg/L+NAA 0.1-0.3mg/L；
1.3rd, strong seedling culture base：MS+6-BA 0.5-1.5mg/L+NAA 0.05-0.15mg/L；
1.4th, root media：MS+NAA 0.1-0.3mg/L；
The pH5.8 of above-mentioned various culture mediums, sucrose 30g/L, agar 6g/L are added, cultivation temperature is 25 ± 1 DEG C, and illumination is
1400-1600Lx；
(2) tissue cultures of brocade flower are changed：
2.1st, the acquisition of sterilizable material
The bead that brocade flower takes aerial part the 10-11 months in the fall is changed, the bulb for peelling off outside rinses 1h after super with running water
On net workbench, 30s is soaked with 75% ethanol, 1 ‰ mercuric chloride soak 15min, aseptic water washing 5-6 times, and aseptic filter paper blots
Surface moisture content, take bead base portion to be cut into 1cm length, be inoculated on bead inducing culture；
2.2nd, the differentiation of bud and propagation
Bead is inoculated on inducing culture after 30 days, and bead base portion starts to expand, and yellow green projection occurs, and being further cultured for 25 days can
See obvious callus, continue culture 30 days, cut dribbling callus and be put on adventitious bud proliferation culture medium and cultivate；
2.3rd, adventitious bud strong seedling culture
On adventitious bud proliferation culture medium, the only 2-3 beads of Multiple Buds every clump induced can extend, and remaining, which is in, downgrades shape
State, after being divided into little Cong or single balls, strong seedling culture base is put into, adventitious bud is extended rapidly, and 2-3cm can be grown to after 20 days；
2.4th, culture of rootage
1-2cm plantlet is taken, root induction in root media of transferring, seedling base portion differentiates many white after 20 days
Root restriction, it can be grown to 1-2cm after 30 days；
2.5th, hardening and transplanting
After culture of rootage 28-32 days, when root system length is to 1-2cm, the aseptic seedling of well developed root system robust growth, indoor corkage refining are selected
Seedling 3 days, root agar is cleaned after taking-up, hardening is tamed in greenhouse after 40 days, be i.e. transplanting is outdoor, gives rich water quality management, transplants
Survival rate is more than 95%.
2. a kind of brocade that changes based on tissue cultures according to claim 1 spends fast breeding method, it is characterised in that described
Bead inducing culture component and content be：MS+BA 3mg/L+NAA 0.3mg/L.
3. a kind of brocade that changes based on tissue cultures according to claim 1 spends fast breeding method, it is characterised in that described
Adventitious bud proliferation culture medium component and content be：MS+6-BA 2.0mg/L+NAA 0.2mg/L.
4. a kind of brocade that changes based on tissue cultures according to claim 1 spends fast breeding method, it is characterised in that described
Strong seedling culture base component and content be：MS+6-BA 1.0mg/L+NAA 0.1mg/L.
5. a kind of brocade that changes based on tissue cultures according to claim 1 spends fast breeding method, it is characterised in that described
Root media component and content be：MS+NAA 0.2mg/L.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109329060A (en) * | 2018-11-21 | 2019-02-15 | 江苏省中国科学院植物研究所 | The method for carrying out tissue-culturing rapid propagation as explant to change brocade flower short-tube lycoris plateau |
| CN110537490A (en) * | 2019-09-20 | 2019-12-06 | 上海上房园林植物研究所有限公司 | method for rapidly breeding floral leaf illicium verum discs through tissue culture |
| CN111109081A (en) * | 2020-01-03 | 2020-05-08 | 上海市农业科学院 | Lycoris radiata rootless tissue culture method and lycoris radiata cultivation method |
| CN112273210A (en) * | 2020-11-13 | 2021-01-29 | 上海上房园艺有限公司 | Seedling hardening method for brocade-changing tissue culture seedlings |
| CN116391624A (en) * | 2023-06-08 | 2023-07-07 | 云南昊辰农业有限公司 | Tissue culture method of New Zealand flax |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960243A (en) * | 2012-06-28 | 2013-03-13 | 浙江农林大学 | Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis |
-
2016
- 2016-09-27 CN CN201610854248.3A patent/CN107864853A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960243A (en) * | 2012-06-28 | 2013-03-13 | 浙江农林大学 | Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109329060A (en) * | 2018-11-21 | 2019-02-15 | 江苏省中国科学院植物研究所 | The method for carrying out tissue-culturing rapid propagation as explant to change brocade flower short-tube lycoris plateau |
| CN110537490A (en) * | 2019-09-20 | 2019-12-06 | 上海上房园林植物研究所有限公司 | method for rapidly breeding floral leaf illicium verum discs through tissue culture |
| CN111109081A (en) * | 2020-01-03 | 2020-05-08 | 上海市农业科学院 | Lycoris radiata rootless tissue culture method and lycoris radiata cultivation method |
| CN112273210A (en) * | 2020-11-13 | 2021-01-29 | 上海上房园艺有限公司 | Seedling hardening method for brocade-changing tissue culture seedlings |
| CN116391624A (en) * | 2023-06-08 | 2023-07-07 | 云南昊辰农业有限公司 | Tissue culture method of New Zealand flax |
| CN116391624B (en) * | 2023-06-08 | 2023-08-18 | 云南昊辰农业有限公司 | Tissue culture method of New Zealand flax |
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Application publication date: 20180403 |