CN107857307A - A kind of new strategy for realizing one kettle way grape grape color developing detection - Google Patents
A kind of new strategy for realizing one kettle way grape grape color developing detection Download PDFInfo
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- CN107857307A CN107857307A CN201711155226.9A CN201711155226A CN107857307A CN 107857307 A CN107857307 A CN 107857307A CN 201711155226 A CN201711155226 A CN 201711155226A CN 107857307 A CN107857307 A CN 107857307A
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
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- B01J23/70—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of the iron group metals or copper
- B01J23/74—Iron group metals
- B01J23/75—Cobalt
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
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- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The present invention relates to nano material, catalysis and analytical chemistry field, specifically includes a kind of new strategy for realizing one kettle way grape grape color developing detection.This method is mixed bovine serum albumin with divalent cobalt ion, normal-temperature reaction generates cobaltosic oxide nano ball under the stabilization of bovine serum albumin after adding sodium borohydride using the nano structure of bovine serum albumin and its property of functional group.For this method using biomaterial as template, preparation technology is simple, and reaction condition is gently environmentally friendly, and nanostructured is easily-controllable.The advantage of the material is the functional group with imitative enzymatic characteristic, magnetic and modified surface, can quickly modify glucose oxidase based on this advantage and be used for recyclable glucose color developing detection.Another considerable advantage of the present invention is the active pH scopes for changing glucose oxidase in the immobilization of cobaltosic oxide nano ball surface using glucose oxidase, so as to realize one kettle way chromogenic assay.Nano material involved by patent has broad application prospects in analytical chemistry, environmental project and catalytic field.
Description
Technical field
The present invention relates to nano material, catalysis and analytical chemistry field, specifically includes one kind and realizes that one kettle way grape grape show
The new strategy of color detection.
Background technology
In recent years, cobaltosic oxide has caused each neck as a kind of metal oxide with essential industry purposes
The extensive concern in domain.Scientific research personnel has synthesized nanometer grade cobaltosic oxide using a variety of methods, by its unique structure and property
Can, it has been widely used in the fields such as battery, capacitor, immunoassay, catalysis.Wei et al. exists《Microwave-
Assisted Synthesis of Mesoporous Co3O4 Nanoflakes for Applications in Lithium
Ion Batteries and Oxygen Evolution Reactions》Closed in one text using microwave hydrothermal solution and low temperature transformation approach
Into porous C o3O4Nanometer sheet, and use it for battery（ACS Applied Materials & Interfaces 2015, 7,
3306-3313）.Rao et al. exists《Ultralayered Co3O4 for High-Performance Supercapacitor
Applications》In one text Co has been synthesized using hydro-thermal method3O4Nanometer sheet, and use it for capacitor（The Journal of Physical Chemistry C2011, 115, 15646-15654）.Zhang et al. exists《Co3O4 Nanoparticles
with Multi-Enzyme Activities and Their Application in Immunohistochemical
Assay》Co deposited synthesis Co is utilized in one text3O4Nano-particle, and use it for immunoassay（ACS Applied Materials & Interfaces 2014, 6, 1959-1970）.Yuichi et al. exists《Morphology Effects of
Co3O4Nanocrystals Catalyzing CO Oxidation in a Dry Reactant Gas Streamw》One text
It is middle to have synthesized Co using hydro-thermal method3O4Nanocrystal, and use it for being catalyzed（Catalysis Science & Technology
2011, 1, 920-922）.He et al. exists《Peroxidase-Like Activity of Co3O4 Nanoparticles
Used for Biodetection and Evaluation of Antioxidant Behavior》Hydro-thermal method is utilized in one text
Co is synthesized3O4Nano-particle, and it is used and is catalyzed（Nanoscale 2016, 8, 5938-5945）.Tang et al. exists
《Porous Co3O4 Nanorods−Reduced Graphene Oxide with Intrinsic Peroxidase-Like
Activity and Catalysis in the Degradation of Methylene Blue》Hydro-thermal method is utilized in one text
Porous C o is synthesized3O4Nanometer rods, and use it for being catalyzed（ACS Applied Materials & Interfaces 2013,
5, 3809-3815）.And the synthesis condition method of these cobaltosic oxides requires that higher and pattern is difficult to control, therefore explore efficient
Cobaltosic oxide synthetic method be a meaningful job.This experiment is intended with bovine serum albumin（BSA）For biological template
Controlledly synthesis is with preferable monodispersity is good, the Co with optimal magnetic property and Mimicry enzyme3O4 NPs。
Glucose is a kind of important living matter, is produced among the main Energy supply material of biology and the key of metabolism
Thing.Glucose sugar in blood of human body（Blood glucose）The relative constancy of content has important meaning to the normal physiological function for maintaining human body
Justice.Diabetes are a kind of hyperglycemias, and serious threat human health.In addition, blood glucose value be less than it is diagnosable during 2.8 mmol/L
For hypoglycemia, the patients such as the low, chronic diarrhea of hypothyroidism, hypoadrenocorticism, liver function are more common in.Cause
This, the measure of blood glucose and the preventing and treating early diagnosed to relevant disease have profound significance.Common glucose color developing detection is
Utilize glucose oxidase initial oxidation glucose production H2O2, the nano material with imitative peroxidase activity is then added, is urged
Change H2O2The detection to glucose is realized indirectly.Liu et al. exists《A V2O3-Ordered Mesoporous Carbon
Composite with Novel Peroxidase-Like Activity Towards the Glucose
Colorimetric Assay》Middle glucose oxidase oxidizing glucose under oxygen existence condition, then add with imitative
The V of peroxidase activity2O3Composite realizes that two-step method detects glucose.Shen et al. exists《Ultrasmall Pt
Nanoclusters as Robust Peroxidase Mimics for Colorimetric Detection of
Glucose in Human Serum》Middle glucose oxidase oxidizing glucose under oxygen existence condition, then adds tool
The Pt NCs for having imitative peroxidase activity realize that two-step method detects glucose.These reports are required for two steps to detect glucose,
Process is more complicated.
In summary, existing be reported in when detecting glucose need to add glucose oxidase, pass through two-step method realization pair
The detection of glucose, process is cumbersome, and enzyme is high to pH area requirement, and reaction condition is harsher, after completion of the reaction not
Enzyme and material can be reclaimed.To realize that one kettle way detects, people's generally use changes with imitative peroxidase activity
The active pH scopes of nano material realize one kettle way（Han, L.et al., Au@Ag Heterogeneous Nanorods
as Nanozyme Interfaces with Peroxidase-Like Activity and Their Application
for One-Pot Analysis of Glucose at Nearly Neutral pH. ACS Applied Materials & Interfaces 2015, 7(26), 14463-14470）, and ignore the active pH models for attempting to change glucose oxidase
Enclose, so as to increase the limitation to nano material, because the active pH scopes for changing nano material are more difficult.
The content of the invention
It is an object of the invention to provide a kind of new strategy for realizing one kettle way grape grape color developing detection.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of method with bovine serum albumin templated synthesis cobaltosic oxide nano ball, it is characterised in that this method includes as follows
Step：
(1) bovine serum albumin is well mixed with the aqueous solution containing divalent cobalt ion, be incubated 1 hour；
(2) a certain amount of sodium borohydride is added into above-mentioned mixed solution and is mixed, oscillating reactions is for a period of time；
(3) above-mentioned reaction solution is centrifuged, removes the supernatant containing unreacted component, collect precipitation, 4 °C preserve with standby；
Also purifying cobaltosic oxide nano ball can be collected by the method dialysed or filtered.
Preferably, the aqueous solution containing divalent cobalt ion can be formulated by cobalt acetate, cobalt chloride etc..
The application of above-mentioned cobaltosic oxide nano ball, it is characterised in that：The cobaltosic oxide nano ball was imitated
The catalytic activity of oxide enzyme, can be as the catalyst of analysis detection；Described cobaltosic oxide nano ball is magnetic, and can return
Receive and utilize；Described cobaltosic oxide nano ball have functional group can modified biological enzyme, and change its active pH scope.
Preferably, after the cobaltosic oxide nano ball modification glucose oxidase catalyst can be used as real by one-step method
Existing glucose detection.
Organicvisualization reagent is added during the detection glucose；The organicvisualization reagent is 2,2 '-connection nitrogen-bis-（3- ethylo benzenes
And thiazoline -6- sulfonic acid）Diamine salts（ABTS）Or 3,3 ', 5,5 '-tetramethyl benzidine（TMB）.
The present invention effect be：
1. the present invention is using biological template method and one-step synthesis method cobaltosic oxide nano ball, synthetic method is simple, it is gentle,
Environmental protection, the shortcomings of process that solves conventional method synthesis cobaltosic oxide nano ball is complicated, energy consumption is big, pattern is unmanageable.
2. the cobaltosic oxide nano ball based on bovine serum albumin template is magnetic in the present invention, recoverable.
3. its surface of cobaltosic oxide nano ball based on bovine serum albumin template, which has, in the present invention can modify glucose
The functional group of oxidizing ferment, and glucose oxidase activity pH scopes can be changed, so as to realize the inspection policies of novelty.
It is a kind of green 4. biological template of the present invention is bovine serum albumin, nontoxic to human body
Biological template, and the cobaltosic oxide nano ball synthesized by biological template method has good biocompatibility, in biology
The biochemical analysis fields such as mark, immunoassay, bio-sensing will have a good application prospect.
Brief description of the drawings
Fig. 1 is the building-up process and GOx immobilization schematic diagrames of cobaltosic oxide nano ball provided in an embodiment of the present invention.
Fig. 2 is the transmission electron microscope photo of cobaltosic oxide nano ball provided in an embodiment of the present invention.
Fig. 3 is the Mimicry enzyme effect using ABTS as substrate of cobaltosic oxide nano ball provided in an embodiment of the present invention
Figure.
Fig. 4 is the Mimicry enzyme design sketch using OPD as substrate of cobaltosic oxide nano ball provided in an embodiment of the present invention.
Fig. 5 is the pH effect of optimization figures of cobaltosic oxide nano ball Mimicry enzyme provided in an embodiment of the present invention.
Fig. 6 is the front and rear pH effect of optimization figures of GOx provided in an embodiment of the present invention modifications.
Fig. 7 is the standard working curve that colour developing standard measure provided in an embodiment of the present invention detects glucose.
Embodiment
In order in depth illustrate present disclosure, some embodiments will be further enumerated below, but the present invention does not limit to
In cited embodiment.Specific experiment condition or method are for example unreceipted in the following example, by the normal condition of this area
Or method is carried out.
Embodiment 1
The preparation of cobaltosic oxide nano ball based on bovine serum albumin template：
(1) by bovine serum albumin（The mg/mL of final concentration 0.2）With the aqueous solution of cobalt chloride（The mM of final concentration 5）It is well mixed, room
Temperature is lower to be incubated 1 h；
(2) aqueous solution and the mixing of sodium borohydride, the h of oscillating reactions 5 are added into above-mentioned mixed solution；
(3) above-mentioned reaction solution is centrifuged, removes the supernatant containing unreacted component, collect precipitation, 4 °C preserve with standby；
Purifying cobaltosic oxide nano ball can be collected by the method dialysed or filtered.Specific building-up process is as shown in Fig. 1.
Embodiment 2
The morphology analysis of cobaltosic oxide nano ball：
By the hanging drop of cobaltosic oxide nano ball on copper mesh, its shape of tem study is utilized after 37 DEG C of dryings
Looks.
As shown in Fig. 2 the spherical looks of obtained cobaltosic oxide nano are homogeneous, the nm of average-size size about 50.
Embodiment 3
The Mimicry enzyme of cobaltosic oxide nano ball：
Experimental system a：Catalystic converter system includes H2O2（1 mM）, above-described embodiment obtain cobaltosic oxide nano ball（20
µg/mL）, organicvisualization reagent ABTS（0.5 mM）With Acetic acid-sodium acetate buffer solution（pH 4.0）.Reacted 20 minutes under 37 °C
Afterwards, it is the spectrogram in the range of 300 800 nm to record it in wavelength using ultraviolet specrophotometer.
Another do according to the facts is tested：Control experiment b, cobaltosic oxide nano ball is replaced with the bovine serum albumin of equivalent, with it is above-mentioned
Spectrogram is recorded after standing 20 minutes under experimental system similarity condition.
As shown in figure 3, experimental system a shows obvious peak near 416 nm, it is higher by a lot, says than experimental system b
Bright bovine serum albumin does not have catalytic activity in itself.In summary, it is good to show that the cobaltosic oxide nano ball of the invention prepared has
Good catalytic activity.
Further to prove the Mimicry enzyme of cobaltosic oxide nano ball, substrate is done with another organicvisualization reagent OPD
Instead of ABTS, respectively with cobaltosic oxide nano ball（Experimental system a）、BSA（Experimental system b）Make catalyst, other experiment bars
Part is consistent with above-mentioned experiment, the nm of spectral scanning range scope 300 800.
As shown in figure 4, experimental system a shows obvious peak near 450 nm, and experimental system b is near 450 nm
Without obvious peak, illustrate cobaltosic oxide nano ball have an activity of obvious imitative peroxidase and BSA in itself to OPD without urging
Change activity.
Embodiment 4
The pH optimizations of cobaltosic oxide nano ball Mimicry enzyme：
Catalystic converter system is to include H2O2（1 mM）, cobaltosic oxide nano ball（20 µg/mL）, organicvisualization reagent OPD（0.5
mM）With different pH buffer solution（PH 1.0 2.0, glycine-HCI buffer solution；PH 3.0 6.0, Acetic acid-sodium acetate buffering
Liquid；PH 7.0 8.0, phosphate buffer；PH 9.0, Tris- hydrochloride buffer；PH 10.0-11.0, sodium carbonate-hydroxide
Sodium buffer solution）.After being reacted 20 minutes under 37 °C, the light absorption value at UV spectrophotometer measuring its 450 nm is utilized.Such as figure
Shown in 5, cobaltosic oxide nano ball shows optimal peroxidase activity at pH 4.0.
Embodiment 5
Modify the preparation of the cobaltosic oxide nano ball of glucose oxidase：
(1) cobaltosic oxide is dissolved in 1mM t-butyl carbamate solution, places 2h, washing, Magnetic Isolation；
(2) cobaltosic oxide that above-mentioned reaction obtains is added to 1- ethyls -3-（3- dimethylaminopropyls）Carbodiimide hydrochloride
In the mixed liquor of N- hydroxyl fourth diimines, lucifuge reaction 5h, Magnetic Isolation, wash, dry；
(3) cobaltosic oxide that above-mentioned reaction obtains is added in glucose oxidase solution, overnight, magnetic is divided for 4 DEG C of reactions
From, obtain modify glucose oxidase cobaltosic oxide nano ball.Specific building-up process is as shown in Fig. 1.
Embodiment 6
The front and rear pH activity optimizations of GOx modifications：
Catalystic converter system is to include glucose（1 mM）, glucose oxidase（20 µg/mL）, cobaltosic oxide nano ball（20
µg/mL）With different pH buffer solution（1.0−11.0）, after reacting 20 minutes, centrifugation, supernatant is taken out, then adds horseradish mistake
Oxide enzyme HRP (20 μ g/mL), organicvisualization reagent OPD（0.5 mM）With Acetic acid-sodium acetate buffer solution（pH 4.0）, at 37 °
After being reacted 20 minutes under C, the light absorption value at its 450 nm is detected using ELIASA.As shown in fig. 6, GOx is showed at pH 7.5
Go out optimal peroxidase activity and be greatly reduced in the activity of pH 4.0 or so.
Catalystic converter system is to include glucose（1 mM）, glucose oxidase modification cobaltosic oxide nano ball（20
µg/mL）With different pH Acetic acid-sodium acetate buffer solution（1.0−11.0）, after reacting 30 minutes, centrifugation, supernatant is taken out, then
Add HRP (20 μ g/mL), organicvisualization reagent OPD（0.5 mM）With Acetic acid-sodium acetate buffer solution（pH 4.0）, under 37 °C
After reaction 20 minutes, the light absorption value at its 450 nm is detected using ELIASA.As shown in fig. 6, glucose oxidase modifies four oxygen
Change the peroxidase activity that three cobalt nanospheres show more than 80% in the range of pH3.0 10.0.
Embodiment 7
Catalystic converter system is the glucose comprising various concentrations（0−10 mM）, glucose oxidase modification cobaltosic oxide
Nanosphere（20 µg/mL）, organicvisualization reagent OPD（0.5 mM）With Acetic acid-sodium acetate buffer solution（pH 5.0）.It is anti-under 37 °C
After answering 20 minutes, the light absorption value at its 450 nm is detected using ELIASA, and thus draw out Glucose standards working curve.Such as
Shown in Fig. 7, until good linear relationship is presented in 9mM working curves.
Claims (5)
- A kind of 1. new strategy for realizing one kettle way grape grape color developing detection, it is characterised in that：With imitative peroxidase activity Cobaltosic oxide nano ball（Co3O4NPs）The glucose oxidase being fixed thereon can be changed（GOx）Active pH scopes, from And make GOx and Co3O4NPs has greater activity in similar pH scopes, realizes one kettle way grape grape color developing detection.
- 2. the Co as described in claim 13O4NPs, it is characterised in that：Described Co3O4NPs makees template conjunction by bovine serum albumin Into, there is magnetic and recoverable, there is the functional group of being easy to modification and available for fixing biology enzyme.
- 3. the Co as described in claim 13O4NPs, it is characterised in that：Described Co3O4NPs makees under the conditions of pH=3.0-5.0 For with peroxidase activity.
- 4. the one kettle way grape grape color developing detection as described in claim 1, it is characterised in that：Only contain in reaction solution and be fixed with GOx Co3O4NPs, organicvisualization reagent（Such as 2,2 '-connection nitrogen-bis-（3- ethyl benzo thiazole phenanthroline -6- sulfonic acid）Diamine salts（ABTS）、 O-phenylenediamine（OPD）Or 3,3 ', 5,5 '-tetramethyl benzidine（TMB）Deng）, glucose, pH buffer solutions.
- 5. the one kettle way grape grape color developing detection as described in claim 1,4, it is characterised in that：Meat is utilized after reaction certain time Eye carries out qualitative analysis, and quantitative analysis is carried out using AAS.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108404911A (en) * | 2018-04-12 | 2018-08-17 | 厦门大学 | A kind of Au/CeO for benzene catalysis oxidation2The preparation method of catalyst |
| CN109254001A (en) * | 2018-10-29 | 2019-01-22 | 青岛农业大学 | A kind of preparation and application of starch quick measuring card |
| CN112710642A (en) * | 2020-12-16 | 2021-04-27 | 青岛农业大学 | Method for detecting glucose and/or hydrogen peroxide based on mimic enzyme |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63291597A (en) * | 1987-05-22 | 1988-11-29 | Meidensha Electric Mfg Co Ltd | Determination of glucose in biospecimen |
| CN103224258A (en) * | 2013-04-26 | 2013-07-31 | 吉林化工学院 | Simple method for preparing Co3O4 nano-spheres and beta-Co(OH)2 micron-flowers |
| CN106904665A (en) * | 2017-03-20 | 2017-06-30 | 南昌航空大学 | A kind of Co that can be used for the shuttle shape pattern of enzyme-free glucose sensor3O4The preparation method of nano material |
| CN107356591A (en) * | 2017-07-17 | 2017-11-17 | 青岛农业大学 | It is a kind of based on imitative enzyme nano material without one pot of glucose color developing detection method of enzyme |
-
2017
- 2017-11-20 CN CN201711155226.9A patent/CN107857307B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63291597A (en) * | 1987-05-22 | 1988-11-29 | Meidensha Electric Mfg Co Ltd | Determination of glucose in biospecimen |
| CN103224258A (en) * | 2013-04-26 | 2013-07-31 | 吉林化工学院 | Simple method for preparing Co3O4 nano-spheres and beta-Co(OH)2 micron-flowers |
| CN106904665A (en) * | 2017-03-20 | 2017-06-30 | 南昌航空大学 | A kind of Co that can be used for the shuttle shape pattern of enzyme-free glucose sensor3O4The preparation method of nano material |
| CN107356591A (en) * | 2017-07-17 | 2017-11-17 | 青岛农业大学 | It is a kind of based on imitative enzyme nano material without one pot of glucose color developing detection method of enzyme |
Non-Patent Citations (2)
| Title |
|---|
| JAE-WOO KIM ET AL.: ""Cobalt oxide hollow nanoparticles derived by bio-templating"", 《CHEM. COMMUN.》 * |
| YANAN DING ET AL.: ""High-performance peroxidase mimics for rapid colorimetric detection of H2O2 and glucose derived from perylene diimides functionalized Co3O4 nanoparticles"", 《MATERIALS SCIENCE AND ENGINEERING C》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108404911A (en) * | 2018-04-12 | 2018-08-17 | 厦门大学 | A kind of Au/CeO for benzene catalysis oxidation2The preparation method of catalyst |
| CN109254001A (en) * | 2018-10-29 | 2019-01-22 | 青岛农业大学 | A kind of preparation and application of starch quick measuring card |
| CN109254001B (en) * | 2018-10-29 | 2021-06-04 | 青岛农业大学 | Preparation and application of starch rapid test card |
| CN112710642A (en) * | 2020-12-16 | 2021-04-27 | 青岛农业大学 | Method for detecting glucose and/or hydrogen peroxide based on mimic enzyme |
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| Publication number | Publication date |
|---|---|
| CN107857307B (en) | 2020-08-07 |
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