CN107841455B - Two-stage operation nucleic acid reaction detection tube - Google Patents
Two-stage operation nucleic acid reaction detection tube Download PDFInfo
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- CN107841455B CN107841455B CN201610838311.4A CN201610838311A CN107841455B CN 107841455 B CN107841455 B CN 107841455B CN 201610838311 A CN201610838311 A CN 201610838311A CN 107841455 B CN107841455 B CN 107841455B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/54—Labware with identification means
- B01L3/545—Labware with identification means for laboratory containers
- B01L3/5453—Labware with identification means for laboratory containers for test tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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- B01L2200/141—Preventing contamination, tampering
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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Abstract
The invention provides a nucleic acid reaction detecting tube with two-stage operation, which comprises a first tube body, a second tube body and a connecting body. Wherein the first tube further comprises a detection space for placing test paper, and the first tube is also used for detecting the occurrence of reaction and observing the result; the second tube body comprises a containing space capable of containing liquid and containing PCR or RT-PCR reagents and target gene segments; the connector comprises a first part and a second part which are respectively connected with the first pipe body and the second pipe body, and the connector further comprises a flow guide unit, a liquid collecting space and a test paper placing space, wherein the test paper placing space is connected with the liquid collecting space. By the operation of the invention, the reaction result of the nucleic acid polymerase chain reaction and the detection of nucleic acid can be directly carried out without transferring liquid.
Description
Technical Field
The invention relates to the field of biology, in particular to a two-stage operation detection tube for carrying out a nucleic acid polymerase chain reaction and detecting a nucleic acid reaction result in sequence without transferring liquid.
Background
Polymerase Chain Reaction (PCR) is a technique for rapidly amplifying DNA signals, and its principle and main operation steps are (a) denaturation: dissociating double-stranded DNA into single-stranded DNA by using high temperature (90-95 ℃), and taking the single-stranded DNA as a copied template; (b) primer bonding (primer annealing): when the temperature is reduced to a proper temperature, the primer can be adhered to the correct target gene position; (c) primer extension (primer extension): the reaction temperature is adjusted to 72 ℃, and magnesium ions are used as enzyme cofactors, so that DNA polymerase successively adheres deoxyribonucleoside triphosphates (hereinafter referred to as dNTPs) to the primers according to the nucleotide combinations on the template, and then synthesizes another new DNA fragment. The signal of the target nucleic acid is amplified by the temperature rise and fall of the three steps, the number of the target nucleic acid can be amplified by one time when the three steps are repeated, if the three steps are set to carry out 40 cycles in total, the number of the target nucleic acid can be amplified by nearly 109 times, and the signal of the target nucleic acid is amplified, so that the PCR technology is widely used as a molecular diagnosis technology for clinical diagnosis at present and can be applied to diagnosis and prognosis evaluation of genetic disease diagnosis, pathogen diagnosis, tumor and cancer and the like. RT-PCR, which is derived from PCR technology, has a similar application principle and is therefore also currently used in large numbers for clinical diagnosis.
At present, most of devices commonly used for PCR or RT-PCR reaction use heat-resistant plastics as test tubes for holding reaction reagents, and the test tubes are repeatedly heated and cooled by temperature control metals so as to reach the reaction temperature of three steps and achieve the effect of amplifying target nucleic acid signals. In the existing system, because the temperature control metal is used as the reaction system, the reaction volume is large, so that the whole temperature control system has to have a large volume-to-heat capacity ratio; in addition, according to the actual operation at present, the number of cycles required for the experiment is about 30 to 35, and the required reaction time is about two to three hours, wherein most of the time is used for waiting for the temperature of the temperature-controlled metal to increase or decrease, so that the reaction time is difficult to be reduced.
Furthermore, since the PCR or RT-PCR generally uses gel electrophoresis (gel electrophoresis) to confirm whether the signal of the target gene is successfully amplified, after the PCR or RT-PCR is completed, a specific volume of the product containing the amplified signal of the target gene (hereinafter referred to as PCR or RT-PCR product) is taken out from the reaction tube and injected into a prepared colloidal well (well), so that the nucleic acid molecule moves in the positive direction under the action of the electric field due to the negative charge of the nucleic acid molecule in neutral or alkaline solution, and the moving speed is inversely proportional to the molecular weight. By the operation of the method, whether the target gene nucleic acid is successfully amplified can be detected, although the detection accuracy by using the colloidal electrophoresis is high, a plurality of hours of reaction time are required from the time of carrying out the PCR or RT-PCR to the time of completing the colloidal electrophoresis, and for some test items needing to know the detection result immediately or in a short time, the time for carrying out the PCR or RT-PCR and the electrophoresis to confirm the test result is too long, so that the auxiliary diagnosis or the test cannot be carried out by using the PCR or RT-PCR mode generally. In addition, when the result is judged by using the colloid electrophoresis mode, the PCR or RT-PCR product is transferred from the reaction tube to the colloid well, and the PCR or RT-PCR product is easily polluted by external substances in the process, so that the misjudgment is caused.
In order to shorten the reaction time, a technique of performing PCR by the principle of thermal convection reaction (hereinafter referred to as thermal convection PCR) has been developed. The technology is designed by Krishan et al, a cylindrical tube of Rayleigh-Benard cell is designed at the earliest, an upper heating source and a lower heating source are matched for temperature control, generally speaking, the temperature of the top end of a liquid surface is maintained to be about 60 ℃, the temperature of the bottom of the liquid surface is about 95 ℃, the liquid in a cavity is driven to flow through the temperature difference generated by the temperature difference of the upper end and the lower end of the cylindrical cavity, and the PCR reaction is carried out, so that the RT-PCR can be carried out in the same way. Subsequently, a derivative technology applying the same principle is developed and commercially used, such as isolated isothermal reaction (iiPCR) using a single-point heating method, which is a polymerase chain reaction performed in a closed capillary tube, or a closed loop flow channel design using a three-point heating source; or a non-contact radiation mode is utilized, and a closed loop design that the heating point is positioned at the center of the cylindrical tube is adopted, so that various designs such as PCR or RT-PCR effects and the like are achieved. By using the method for carrying out PCR or RT-PCR by utilizing the thermal convection reaction principle, the temperature of the reaction in three steps can be reached without repeatedly heating and cooling the test tube by temperature control metal, so that a lot of time for repeatedly heating and cooling can be saved, and the using amount of the temperature control metal can be reduced.
Meanwhile, in order to reduce the misjudgment of the detection result caused by the contamination of the PCR or RT-PCR products by external substances, in recent years, there is a development of an interpretation technology which can replace the traditional colloid electrophoresis, for example, a single fluorescent substance is combined with a target gene sequence, when a light beam with a proper wavelength is given by a detection instrument to excite the fluorescent substance, the fluorescent substance emits fluorescence and is detected by the detection instrument, the fluorescence brightness is in direct proportion to the quantity of the PCR or RT-PCR products, so that the effect of real-time qualitative or even quantitative can be achieved, and the reaction time can be saved.
In addition, for example, the detection of PCR or RT-PCR products by a test strip with specific markers for target gene sequences is also one of the detection methods commonly used in recent years. The detection principle of the test paper method is that at least two antigens with different specificity antibodies are added into a PCR or RT-PCR reagent, the first antigen can be DIG or TexasR, the second antigen can be Avidin or FITC, and other antigens with specificity can also be used for replacing the antigens. When the reaction is complete, the product is loaded with two different antigens. Meanwhile, one end of the test paper is sprayed with colloidal gold, latex balls or other color-producing substances, the color-producing substances are combined with the specific antibody of the second antigen, the antibody can be Biotin, anti-FITC or other corresponding specific antibodies, and the other end of the test paper is provided with adhered absorbent cotton; in addition, another specific antibody corresponding to the first antigen, such as anti-DIG, anti-TexasR or other corresponding specific antibodies, is sprayed and fixed on the specific part of the test paper; after the reaction is finished, taking out the PCR or RT-PCR product and dropping a reagent with a proper volume at one end of the test paper with the color generation substance sprayed, wherein the first antigen in the product can be specifically combined with the first antibody to form a compound of the first antigen-the first antibody-the color generation substance, the compound can move from the color generation end to the absorbent cotton end due to the capillary phenomenon of the reagent, and when the compound moves to the position where the second antibody is sprayed, the second antigen can be specifically combined with the second antibody and then can be fixed at the position and generate color, so that a user can judge the result according to the color generation. The time required for detecting by using the test paper is about several minutes, which saves much experimental time compared with the traditional colloidal electrophoresis detection method, and the specific antibody can be replaced by a nucleic acid probe according to different requirements.
In the above, the detection method using the test paper assisted by the heat convection PCR and the detection method using the test paper can save a lot of reaction time compared with the traditional detection methods using the PCR, the RT-PCR and the colloidal electrophoresis, although the detection method is widely researched and commercially used, the detection test tube which can directly perform two different operation requirements of the heat convection PCR, the RT-PCR and the product detection is not successfully developed in the current workshop, so the nucleic acid detection can be completed only by taking the product out of the test tube and dropping the product onto the test paper with a proper volume after the heat convection PCR is completed in the test tube, and the operation is quite inconvenient due to the difficulty in taking the product out, and the pollution caused by external substances cannot be avoided.
Accordingly, the present invention provides a two-stage operation detecting tube which can detect the result of a nucleic acid reaction using a test strip in the same device after completion of a PCR or RT-PCR reaction without transferring a reagent or a liquid.
Disclosure of Invention
In view of the above, the present invention relates to a two-stage operation test tube. The detection tube does not need reagent or liquid transfer in use, and can be connected with a connecting tube and a detection tube which are provided with test paper only after the heat convection type PCR or RT-PCR reaction is finished, so that the test paper can be directly used for detecting the nucleic acid reaction result in the same device.
To achieve the above objects, according to a preferred embodiment of the present invention, the apparatus comprises a first tube having a first connecting portion and a detecting space, wherein the detecting space can accommodate a test strip having absorbent cotton at one end; a second tube having a second connection portion and a receiving space for receiving the target gene analyte and the related reagent; a connector having a first portion and a second portion, which is respectively connected to the first connecting portion of the first tube and the second connecting portion of the second tube; the first part of the connector is provided with a flow guide unit and a liquid collection space, the flow guide unit can guide the liquid in the accommodating space to the liquid collection space, the second part of the connector is provided with a test paper placing space for placing the test paper, and the test paper placing space is connected with the liquid collection space.
In one embodiment, the outer peripheral surface of the first tube is substantially a circular tube, and the detection space is an oblong space having two opposite planes.
In one embodiment, the second tube further has a capillary portion.
In one embodiment, the reagent holding space of the connector is an elongated channel with a uniform diameter.
In one embodiment, the liquid collecting space has an outlet for guiding the liquid out, and the outlet is located at the long side of the test paper placing space.
In one embodiment, the liquid collecting space is a cavity connected to the test paper placing space.
In one embodiment, the liquid collecting space has an inlet adjacent to the flow guide unit and an outlet adjacent to the test paper placing space, and the outlet has a larger opening area than the inlet.
In one embodiment, the flow guiding unit is a flow guiding slope surface facing the liquid collecting space and can guide the liquid in the second pipe body to flow into the liquid collecting space.
In one embodiment, the first connecting portion and the second connecting portion have a first engaging structure and a second engaging structure, respectively, for connecting the connecting body.
In one embodiment, the first engaging structure and the second engaging structure are at least one annular protrusion unit for connecting the connecting body.
After the heat convection type PCR or RT-PCR reaction is finished, the first tube body, the second tube body and the connector are assembled to form a closed detection tube, and the detection test paper is placed into the first tube body and the connector in advance. After the assembly is finished, the detection tube is turned over by 180 degrees, so that the reagent enters the liquid collection space through the flow guide unit of the connector and contacts the test paper in the test paper placement space. The capillary phenomenon generated by attaching the absorbent cotton to one end of the test paper pulls the PCR or RT-PCR product from the end of the test paper, which is sprayed with the color-generating substance, to the absorbent cotton end, in the process, the specific antigen carried by the product is combined with the specific antibody sprayed on the test paper in advance to generate color, and a user can observe the detection result through the observation part of the first pipe body. By the technical means disclosed by the invention, the purposes of carrying out heat convection type PCR or RT-PCR and detecting products in the same device can be achieved.
Drawings
FIG. 1 is an exploded view and assembled view of the components of a preferred embodiment of the present invention.
Fig. 2A is a sectional view taken along the direction B-B of the first tubular body 10 of the present invention.
Fig. 2B is a sectional view taken along the line a-a of the first tubular body 10 of the present invention.
Fig. 3 is a top view of the first tube of the present invention.
Fig. 4 is a cross-sectional view taken along the line C-C of the second tubular body of the present invention.
FIG. 5 is a cross-sectional view taken along the direction D-D of the linker of the invention.
Reference numerals
1 detection tube
10 first pipe body
101 first connection part
102 observation part
103 hand-held part
104 detection space
105 first fitting structure
1051 annular bulge unit
20 connecting body
201 first part
202 second part
203 third fitting structure
2031 annular bulge unit
204 flow guiding unit
2041 flow guiding slope
205 liquid collecting space
206 test paper placing space
207 opening
30 second tube
301 second connection part
302 capillary part
303 accommodating space
304 second engaging structure
3041 an annular projection unit
Detailed Description
The structure and function of a preferred embodiment of the present invention will be described in detail below with reference to the accompanying drawings. In addition, in the present specification, the terms "front", "rear", "left", "right", "up", "down", and the like are used to describe the mechanism or the portion thereof in terms of its position, and correspond to the spatial relationship of the user when operating the preferred embodiment.
Referring to fig. 1, a detecting tube 1 according to a preferred embodiment of the present invention mainly includes a first tube 10, a second tube 30, and a connecting body 20 connected between the first tube and the second tube.
Referring to fig. 1, fig. 2A, fig. 2B and fig. 3, in the present embodiment, the first tube 10 is a transparent tube made of PC plastic, and has a first connecting portion 101, a handheld portion 103, an observing portion 102 located between the first connecting portion 101 and the handheld portion 103, and a detecting space 104. In this embodiment, the outer periphery of the observation portion 102 of the first tube 10 is generally a circular periphery with a smooth surface; in addition, one end of the first tube 10 is the above-mentioned hand-held portion 103, and the outer circumference of the tube has two opposite planes, which can be clamped by the fingers of the user for easy interpretation; the first connecting portion 101 at the opposite end of the handle portion 103 has a first engaging structure 105 at the inner side of the tube, in this embodiment, the first engaging structure 105 has at least one annular protruding unit 1051 for connecting the connecting body 20; the inside of the first tube 10 is the detection space 104 for accommodating a test paper with absorbent cotton attached to the tail end, and the test paper is a space with an oblong cross section, that is, the space has two parallel walls and two curved surfaces at opposite positions. Therefore, when the user views the first tube 10 through the observation portion 102, the circular outer peripheral surface and the flat inner wall surface form a plano-convex lens structure, and when the test strip is placed in the detection space 104, the information on the test strip can be magnified for easy interpretation. In addition, the test paper with absorbent cotton attached to the tail end is disposed in the detection space 104 in a direction that one end with absorbent cotton is located at the other end of the first embedding structure 105.
Referring to fig. 1 and 4, the second tube 30 of the present embodiment is a transparent hollow tube made of PC plastic, one end of which has a wider inner diameter for connecting the connector 20, and the opposite end of which has a capillary portion 302 with a narrower inner diameter, and the second tube 30 has a second connecting portion 301 and a receiving space 303. In this embodiment, the second connecting portion 301 is similar to the first connecting portion 101 of the first tube 10, and the inner side of the tube has a second engaging mechanism 304, and the second engaging mechanism has at least one annular protruding unit 3041 for connecting with the connector 20; the accommodating space 303 inside the second tube 30 is used for accommodating a liquid to be detected, such as a reaction reagent.
Referring to fig. 1 and 5, the connector 20 of the present embodiment is an elastic body made of silicone and having a lower hardness than the first tube 10 and the second tube 30, the connector 20 has a first portion 201 and a second portion 202, which can be respectively connected to the first connecting portion 101 of the first tube 10 and the second connecting portion 301 of the second tube 30, specifically, the first portion 201 and the second portion 202 respectively have a third engaging mechanism 203, which is a plurality of annular protruding units 2031 on the outer peripheral surface in the present embodiment, when the connector is connected to the first tube 10 and the second tube 30, the annular protruding unit 1051 on the inner wall of the first tube 10 or the annular protruding unit 3041 on the inner wall of the second tube 30 can force the corresponding portion of the connector 20 to be slightly deformed, so that the annular protruding units on the first portion 201 and the second portion 202 and the corresponding annular protruding units 1051 on the connecting portion of the first tube 10, the second tube 30, respectively, The annular protruding units 3041 of the connecting portion of the second tube 30 are fitted to each other to form a tightly connected and sealed state.
Referring to FIG. 5, the first portion 201 has a flow guide unit 204 and a liquid collection space 205, and the second portion 202 has a test paper placing space 206, which is a long channel with a uniform diameter. The test paper placing space 206 has an opening 207 for pushing the test paper into the test paper placing space 206, the opening is located near the inlet of the diversion unit and near the outlet of the test paper placing space, the length and width of the opening 207 is approximately equal to the width and thickness of the test paper, and the opening area of the outlet is larger than the inlet, so as to prevent the reaction reagent from directly flowing to the detection space 104 of the first tube 10 through the gap to cause misjudgment of the detection result when the reaction reagent does not contact with the test paper and moves to the other end of the test paper through capillary phenomenon to gradually react with the specific antibody sprayed on the test paper.
In this embodiment, the flow guiding unit 204 is a flow guiding slope 2041 facing the liquid collecting space 205, when the user turns the detecting tube 1 upside down by 180 degrees, i.e. turns the detecting tube 1 upside down, the flow guiding slope 2041 can guide the liquid in the second tube 202 to flow to the liquid collecting space 205, the liquid collecting space 205 is a hollow cavity capable of containing the liquid, and the liquid collecting space 205 is communicated with the test paper placing space 206, when the liquid flows into the liquid collecting space 205, the liquid will contact the test paper placed in the test paper placing space 206, and then the detection reaction of PCR or RT-PCR products is performed. In addition, the inlet of the liquid collecting space 205, i.e. the opening area adjacent to the flow guiding unit 204, is narrower than the outlet of the liquid collecting space 205, i.e. the opening area adjacent to the test paper placing space 206, so that the liquid can smoothly flow in a fixed direction. In order to accelerate the reaction and increase the contact area between the liquid and the test paper, the outlet of the liquid collecting space 205 is disposed on the long side of the test paper placing space 206, so that the liquid can directly contact the test paper in a large range when flowing out of the liquid collecting space 205, thereby accelerating the reaction.
In use, a target gene fragment containing a target gene to be analyzed, a specific antigen and other reagents are injected into the second tube body 30, and heat convection type PCR or RT-PCR is carried out, wherein when the reaction is finished, a PCR or RT-PCR product and two antigens, namely DIG and Avidin, are carried out simultaneously; meanwhile, the test paper coated with the color substance and the specific antibody and having the tail end attached with the absorbent cotton is placed in the test paper placing space 206 of the connector 20, and then the connector 20 is connected and assembled with the second connecting portion 301 of the second tube 30, so that the test paper is placed in the accommodating space 303 of the second tube 30 and fixed. In this embodiment, the coloring material is colloidal gold, one specific antigen is DIG, the other specific antigen is Avidin, the corresponding specific antibodies are Anti-DIG and Biotin, and the Biotin and the colloidal gold are combined to form a colloidal gold-Biotin complex.
After the completion of the thermal convection PCR or RT-PCR reaction, the connector 20 and the first tube 10 are directly assembled and connected according to the first tube 10, the connector 20 and the second tube 30 shown in fig. 1 without removing the reaction reagent of the second tube 30, so that the detecting tube 1 becomes a completely closed tube. Then, the detecting tube 1 is inverted by 180 degrees, that is, the detecting tube 1 is turned upside down in fig. 1, and at this time, the reagent containing the thermal convection PCR or RT-PCR product originally located in the second tube 30 flows to the liquid collecting space 205 and the test paper placing space 206 along the flow guiding slope 2041, and contacts the test paper to be tested in the test paper placing space 206. Since the absorbent cotton is attached to one end of the test strip, the reagent containing the PCR or RT-PCR product will gradually move from the end of the test strip placement space 206 toward the absorbent cotton due to the influence of capillary phenomenon. In the moving process of the reagent with PCR or RT-PCR products, the Avidin is specifically combined with the Biotin and forms an Avidin-Biotin-colloidal gold complex, the complex continuously moves to the absorbent cotton end due to capillary phenomenon, when the complex moves to the position where Anti-DIG is sprayed, the DIG is fixed and colored after being specifically combined with the conjugate, and a user can judge the result according to whether the color is formed or not.
The above-mentioned embodiments are merely preferred embodiments of the present invention, and all equivalent changes and modifications made by the claims of the present invention should be covered by the scope of the present invention.
Claims (8)
1. A two-stage nucleic acid reaction detecting tube, comprising:
the test paper detection device comprises a first pipe body and a second pipe body, wherein the first pipe body is provided with a first connecting part and a detection space, and the detection space can contain test paper;
the second pipe body is provided with a second connecting part and an accommodating space, and the accommodating space can accommodate liquid; and
the connecting body is provided with a first part and a second part which are respectively connected with the first connecting part of the first pipe body and the second connecting part of the second pipe body, the first part of the connecting body is provided with a flow guide unit and a liquid collecting space, the flow guide unit can guide the liquid in the accommodating space to the liquid collecting space, the second part is provided with a test paper placing space, and the test paper placing space is connected with the liquid collecting space;
the liquid collecting space is a cavity and is connected with the test paper placing space;
the liquid collecting space is provided with an inlet adjacent to the flow guide unit and an outlet adjacent to the test paper placing space, and the opening area of the outlet is larger than that of the inlet.
2. The two-stage nucleic acid reaction detecting tube according to claim 1, wherein the outer peripheral surface of the first tube has a circular tube shape, and the detecting space has an oblong space with two opposing flat surfaces.
3. The two-stage nucleic acid reaction detecting tube according to claim 1, wherein the second tube further has a capillary portion.
4. The two-stage nucleic acid reaction detecting tube according to claim 1, wherein the reagent holding space of the connecting body is an elongated channel having a uniform tube diameter.
5. The two-stage nucleic acid reaction detecting tube according to claim 4, wherein the liquid collecting space has an outlet for discharging the liquid, and the position of the outlet is located at a long side of the test strip housing space.
6. The two-stage operation nucleic acid reaction detecting tube according to claim 1, wherein the flow guide unit is a flow guide slope surface facing the liquid collecting space and guides the liquid in the second tube body to flow into the liquid collecting space.
7. The two-stage nucleic acid reaction detecting tube according to claim 1, wherein the first connecting portion and the second connecting portion have a first engaging structure and a second engaging structure, respectively, for connecting the connecting body.
8. The two-stage nucleic acid reaction detecting tube according to claim 7, wherein the first and second engaging structures are at least one annular protrusion unit for connecting the connecting body.
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| CN102803465A (en) * | 2010-01-12 | 2012-11-28 | 阿赫姆生物系统公司 | Two-stage thermal convection apparatus and uses thereof |
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| CN104293659A (en) * | 2013-07-19 | 2015-01-21 | 厦门万泰沧海生物技术有限公司 | Fully enclosed integrated nucleic acid amplification and detection tube |
| CN206308356U (en) * | 2016-09-21 | 2017-07-07 | 克雷多生物医学私人有限公司 | A kind of two benches operate nucleic acid reaction detection pipe |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040110167A1 (en) * | 1995-07-13 | 2004-06-10 | Gerdes John C. | Lateral flow system for nucleic acid detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102803465A (en) * | 2010-01-12 | 2012-11-28 | 阿赫姆生物系统公司 | Two-stage thermal convection apparatus and uses thereof |
| CN103484353A (en) * | 2012-06-12 | 2014-01-01 | 清华大学 | Biomacromolecule extracting device based on filter paper |
| CN104293659A (en) * | 2013-07-19 | 2015-01-21 | 厦门万泰沧海生物技术有限公司 | Fully enclosed integrated nucleic acid amplification and detection tube |
| CN103674945A (en) * | 2013-12-10 | 2014-03-26 | 复旦大学 | Organic phosphorus detection system and method |
| CN206308356U (en) * | 2016-09-21 | 2017-07-07 | 克雷多生物医学私人有限公司 | A kind of two benches operate nucleic acid reaction detection pipe |
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