CN107841454A - Cell scraper cutter head and cell scraper - Google Patents
Cell scraper cutter head and cell scraper Download PDFInfo
- Publication number
- CN107841454A CN107841454A CN201711384389.4A CN201711384389A CN107841454A CN 107841454 A CN107841454 A CN 107841454A CN 201711384389 A CN201711384389 A CN 201711384389A CN 107841454 A CN107841454 A CN 107841454A
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- CN
- China
- Prior art keywords
- knife
- handle
- cell
- cell scraper
- blade
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000007789 sealing Methods 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims 1
- -1 polyethylene Polymers 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000012216 screening Methods 0.000 abstract description 5
- 238000005452 bending Methods 0.000 description 9
- 230000006378 damage Effects 0.000 description 7
- 239000006285 cell suspension Substances 0.000 description 6
- 238000010494 dissociation reaction Methods 0.000 description 6
- 230000005593 dissociations Effects 0.000 description 6
- 229920001903 high density polyethylene Polymers 0.000 description 6
- 239000004700 high-density polyethylene Substances 0.000 description 6
- 230000001079 digestive effect Effects 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000007790 scraping Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 2
- 238000010009 beating Methods 0.000 description 2
- 238000007664 blowing Methods 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009643 clonogenic assay Methods 0.000 description 1
- 231100000096 clonogenic assay Toxicity 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
Abstract
The invention provides a kind of cell scraper cutter head and cell scraper, it is related to experiment instrument field.The cell scraper cutter head includes blade and connection handle；The knife-edge part of the blade is provided with multiple holes；The connection handle is internally provided with the hollow pipeline through both ends；One end of the connection handle and the side of the blade are affixed, and multiple described holes connect with the hollow pipeline；The other end of the hollow pipeline is used to be connected with handle of a knife.The cell scraper includes cell scraper cutter head as described above, in addition to handle of a knife；Piston hole is set inside the handle of a knife, and the piston hole is internally provided with piston；Described handle of a knife one end is fitted with piston rod, and the piston rod and the piston are affixed；The other end of the handle of a knife is connected with the end of the connection handle, and the piston hole connects the present invention with hollow pipeline sealing and solves existing cell scraper, can only scrape, it is impossible to collect；The low technical problem of cell line screening rate.
Description
Technical field
The present invention relates to experiment equipment technology field, more particularly, to a kind of cell scraper cutter head and cell scraper.
Background technology
Cell scraper is a kind of conventional cell manipulation instrument in cell culture, is mainly used in the collection of cell.Traditional
The blade of cell scraper is in line type, and blade is connected with handle of a knife, it is impossible to dismantles, in scraping cells bottle or culture plate
In (96 holes, 24 holes, 12 holes, 6 holes) during cell, it is impossible to which with allowing blade replacement, and blade can not be good with the edge of culture vessel
Contact, the function of scraper is limited, therefore influences to collect the performance of cell collecting effect and function.
Based on this, the invention provides a kind of cell scraper cutter head and cell scraper to solve above-mentioned technical problem.
The content of the invention
It is an object of the invention to provide a kind of cell scraper cutter head, to solve existing cell scraper cell line screening rate
Low technical problem.
The present invention also aims to provide a kind of cell scraper, for solving above-mentioned technical problem.
Based on above-mentioned first purpose, the invention provides a kind of cell scraper cutter head, including blade and connection handle；
The knife-edge part of the blade is provided with multiple holes；The connection handle is internally provided with the hollow tube through both ends
Road；
One end of the connection handle and the side of the blade are affixed, and multiple described holes connect with the hollow pipeline；
The other end of the hollow pipeline is used to be connected with handle of a knife.
Optionally, length direction of much individual described holes along the edge of a knife is evenly arranged.
Optionally, for the connection handle in cone, its thinner one end and the blade are affixed.
Optionally, the edge of a knife is in yi word pattern or circular arc.
Optionally, the blade and the connection handle are integrally formed using high density polyethylene (HDPE) material.
Optionally, the diameter of multiple described holes is 200um~500um.
Based on above-mentioned second purpose, the invention provides a kind of cell scraper, the cell scraper includes as described above
Cell scraper cutter head, in addition to handle of a knife；
Piston hole is set inside the handle of a knife, and the piston hole is internally provided with piston；
Described handle of a knife one end is fitted with piston rod, and the piston rod and the piston are affixed；The other end of the handle of a knife with
The end connection of the connection handle, and the piston hole connects with hollow pipeline sealing.
Optionally, connection handle described in the handle of a knife is detachably connected.
Optionally, the end set of the connection handle has slot, and the handle of a knife is provided with plug, and the plug is inserted into institute
State in slot.
Optionally, the handle of a knife uses transparent material, and the length direction of the piston hole is provided with scale.
Single cell clone is carried out using cell scraper of the present invention.Specific method is as follows：Cell is pressed per hole 3~5
Individual cell is inoculated into 96 orifice plates, 37 DEG C of 5%CO2Quiescent culture 15 days in incubator.Daily observation cell situation.According to cell
Form, growth rate, the size of monoclonal and individual cells position distribution are struck off to unnecessary single cell clone strain.Tool
Gymnastics is made：(1) cell strikes off, and finds cell hole under the microscope, blade is moved in the visual field.Slow moving blade strikes off
Unwanted single cell clone strain, the cell struck off is directly drawn by scraper, retains desired single cell clone.(2) cell
Digestion, after single cell clone grow to it is sufficiently large after, discard nutrient solution.Cell dissociation buffer is injected to cell training from hollow pipeline
Support in hole, carry out cell dissociation.After cell dissociation to individual cells, cell no longer connect in flakes, digestive juice is sucked.Add new
Fresh nutrient solution blade blows and beats cell uniformly, and is transferred in cell culture vessel and amplifies culture.It is slender due to generally carrying out
When born of the same parents clone, it is necessary to assure per 1, hole cell, more than 1 cell is just needed unnecessary cell clearance by the operation of complexity；
The present invention substantially reduces to the quantitative requirement of every hole cell, and can increase to 5 from every 1 cell in hole even more more (is no more than
10) required so as to reduce single cell clone condition.In addition, when generally carrying out single cell clone, typically to the thin of harvest
Born of the same parents directly scrape, and cell mechanical injuries are larger；The present invention not only can be scraped directly, and can also be by adding pancreas
Protease digestion cell, then by blowing and beating cell, the mode for drawing cell suspension collects cell, hence it is evident that reduces the damage of cell
Wound, improve Cell viability.Therefore, in single cell clone experiment, unwanted cells strain can be not only removed, and reduce cell
Artificial damage during collection, substantially increase the operating efficiency of cell line screening.Compared with traditional cell scraper, operation is more square
Just, with better function, cell acquisition rate is high.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the schematic diagram of cell scraper cutter head provided in an embodiment of the present invention；
Fig. 2 is the schematic diagram of the circular arc edge of a knife of the cell scraper cutter head shown in Fig. 1；
Fig. 3 is the schematic diagram of the in-line edge of a knife of the cell scraper cutter head shown in Fig. 1；
Fig. 4 is the schematic diagram of cell scraper provided in an embodiment of the present invention.
Icon：1- blades；2- connection handles；3- holes；4- hollow pipelines；5- handle of a knifes；6- piston holes；7- piston rods；8- is inserted
Groove；9- plugs.
Embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
In the description of the invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Be easy to the description present invention and simplify description, rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for describing purpose, and it is not intended that instruction or hint relative importance.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can
To be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected by intermediary, Ke Yishi
The connection of two element internals.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
Cell scraper cutter head embodiment one
As shown in figure 1, providing a kind of cell scraper cutter head in the present embodiment, the cell scraper cutter head includes blade
1 and connection handle 2；
The knife-edge part of the blade 1 is provided with multiple holes 3；The connection handle 2 is internally provided with through the hollow of both ends
Pipeline 4；
One end of the connection handle 2 and the side of the blade 1 are affixed, and multiple described holes 3 connect with the hollow pipeline 4
It is logical；
The other end of the hollow pipeline 4 is used to be connected with handle of a knife.
Single cell clone is carried out using cell scraper of the present invention.Specific method is as follows：Cell is pressed per hole 3~5
Individual cell is inoculated into 96 orifice plates, quiescent culture 15 days in 37 DEG C of 5%CO2 incubators.Daily observation cell situation.According to cell
Form, growth rate, the size of monoclonal and individual cells position distribution are struck off to unnecessary single cell clone strain.Tool
Gymnastics is made：(1) cell strikes off, and finds cell hole under the microscope, blade is moved in the visual field.Slow moving blade strikes off
Unwanted single cell clone strain, the cell struck off is directly drawn by scraper, retains desired single cell clone.(2) cell
Digestion, after single cell clone grow to it is sufficiently large after, discard nutrient solution.Cell dissociation buffer is injected to cell training from hollow pipeline
Support in hole, carry out cell dissociation.After cell dissociation to individual cells, cell no longer connect in flakes, digestive juice is sucked.Add new
Fresh nutrient solution blade blows and beats cell uniformly, and is transferred in cell culture vessel and amplifies culture.It is slender due to generally carrying out
When born of the same parents clone, it is necessary to assure per 1, hole cell, more than 1 cell is just needed unnecessary cell clearance by the operation of complexity；
The present invention substantially reduces to the quantitative requirement of every hole cell, and can increase to 5 from every 1 cell in hole even more more (is no more than
10) required so as to reduce single cell clone condition.In addition, when generally carrying out single cell clone, typically to the thin of harvest
Born of the same parents directly scrape, and cell mechanical injuries are larger；The present invention is by adding trypsin digestion and cell, then by blowing and beating carefully
Born of the same parents, the mode for drawing cell suspension collect cell, hence it is evident that reduce the damage of cell, improve Cell viability.Therefore, unicellular
In clonogenic assay, unwanted cells strain can be not only removed, and reduces artificial damage when cell is collected, is substantially increased thin
The operating efficiency of born of the same parents' strain screening.Easy to operate, powerful compared with traditional cell scraper, cell acquisition rate is high.
Such as Fig. 2,3, in the alternative of the present embodiment, length direction of much individual described holes 3 along the edge of a knife is uniform
Arrangement.
The hole 3 being evenly arranged is easy to process, moreover, either injection digestive juice still extracts cell suspension, can
Rapidly and uniformly extraction.
Such as Fig. 1, in the alternative of the present embodiment, the connection handle 2 is in cone, its thinner one end and the blade
1 is affixed.
Connection handle is arranged such, is easy to be connected with handle of a knife.
Such as Fig. 2,3, in the alternative of the present embodiment, the edge of a knife is in yi word pattern or circular arc.
Existing scraper can not contact well with the edge of culture vessel, and the function of scraper is limited, therefore influence to collect
The performance of cell collecting effect and function.For different culture vessels, using cutter head of different shapes and the edge of a knife, be advantageous to thin
The collection of born of the same parents.According to experiment demand, the edge of a knife is in yi word pattern or circular arc type, and the length of blade 1 can have plurality of specifications, such as 2mm
~2cm.
In the alternative of the present embodiment, the blade 1 and the connection handle 2 are using high density polyethylene (HDPE) material one
Shaping.
Integrally formed structure bonding strength is high, can application time it is longer.And high density polyethylene (HDPE) material wearability is tough
Property etc. all preferably, it is relatively durable.
In the alternative of the present embodiment, the diameter of multiple described holes 3 is 200um~500um.
Multiple described holes 3 are rounded, and 200um~500um diameter is enough to draw cell suspension,
Cell scraper embodiment
As shown in figure 4, this embodiment offers a kind of cell scraper, the cell scraper is scraped including cell as described above
Knife cutter head, in addition to handle of a knife 5；
The inside of handle of a knife 5 sets piston hole 6, and the piston hole 6 is internally provided with piston；
Described one end of handle of a knife 5 is fitted with piston rod 7, and the piston rod 7 and the piston are affixed；The handle of a knife 5 it is another
End is connected with the end of the connection handle 2, and the piston hole 6 connects with the hollow pipeline 4 sealing.
Handle of a knife is in hollow, piston type cylinder.Short and thin opening is contained at top, is connected with cutter head.Contain one in handle of a knife
Piston rod 7, the upper piston of piston rod 7 are isoprene material.Cutter head, handle of a knife and piston rod 7 form a semiclosed secret room, are used for
The absorption of cell suspension.The length of handle of a knife and piston rod 7 can have all size, such as 5cm, 10cm, 20cm.Pass through piston rod
7 twitch, can complete to extract cell suspension out and inject digestive juice, the cell scraper of the application can be completed by itself this two
Individual operation, and existing scraper does not possess above-mentioned function.In single cell clone experiment, unwanted cells strain is removed, significantly
Improve the operating efficiency of cell line screening.More convenient operation, with better function compared with traditional cell scraper, cell obtains
Take rate higher.
The scraper of the application is not only able to direct scraping cells, but also can inject digestive juice vitellophag, and will be thin
Born of the same parents' suspension suctions out, and alleviates by scraping the damage to caused by cell.With the characteristics of full-featured, easy to operate.Except this it
Outside, when carrying out primitive cell culture, there is the simultaneous situation of various kinds of cell for cell, can using the scraper of the application
To carry out removal rapidly and efficiently, save trouble, it is laborsaving.
Such as Fig. 4, in the alternative of the present embodiment, connection handle 2 described in the handle of a knife 5 is detachably connected.
The blade 1 of traditional cell scraper is in line type, and blade 1 is connected with handle of a knife 5, it is impossible to dismantles, is scraping
When taking cell in cell bottle or culture plate (96 holes, 24 holes, 12 holes, 6 holes), it is impossible to which with allowing blade replacement 1, and blade 1 can not be with training
The edge for supporting container contacts well, and the function of scraper is limited, therefore influences to collect the performance of cell collecting effect and function.This
Blade 1 is arranged to removably by application, can take different assembling modes according to the difference of culture vessel, effectively improve
Cell harvesting rate.
Further, the end set of the connection handle 2 has slot 8, and the handle of a knife 5 is provided with plug 9, the plug 9
It is inserted into the slot 8.
The mode of inserting connect it is more simple, moreover, sealing effectiveness is preferable, can make piston piston hole 6 with it is described hollow
The sealing connection of pipeline 4
Preferably, the handle of a knife 5 uses transparent material, and the length direction of the piston piston hole 6 is provided with scale.
The amount of liquid being injected and extracted from can be observed by blocking up user by the shell, convenient to practice data statistics.
Regard blade of the present invention as linear elastomer, regard the cross section of the edge of a knife as under external force indeformable circle and cut
Face, draws the stereochemical structure model of the cross section composition of circular arc type blade and the edge of a knife, and derives T theoretical calculation formulas.Small
Under deformation condition, edge of a knife moment M linearly changes (see formula) with bending curvature K, and wherein B is bending stiffness, because blade is curved
The curvature relationship of square one is in fact nonlinear, so under big strained situation, its resultant error is larger.K=M/B.Due to wanting
Overcome friction work between blade edge and vessel surface, the bending hysteresis quality of blade and non-linear is considered, using blade to be non-thread
Property model.Do not slid between blade and vessel surface, blade has very high bending stiffness, when more than coercive torque it
Afterwards, moment of flexure and the linear relation of curvature, its mathematic(al) representation:K=0 (M≤M0) K=(M-M0)/B(M≦M0) in formula:K is
Blade bending curvature, M are external force action moment, M0For coercive torque, B is blade bending stiffness.By assessing blade and blade
The bending stiffness of circular arc type cross section, judge that the bending stiffness of blade characterizes its bending property.
Finally it should be noted that：It is obvious to a person skilled in the art that the invention is not restricted to above-mentioned exemplary implementation
The details of example, and without departing from the spirit or essential characteristics of the present invention, can realize in other specific forms
The present invention.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this hair
Bright scope limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained
All changes in justice and scope are included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (10)
1. a kind of cell scraper cutter head, it is characterised in that including blade and connection handle；
The knife-edge part of the blade is provided with multiple holes；The connection handle is internally provided with the hollow pipeline through both ends；
One end of the connection handle and the side of the blade are affixed, and multiple described holes connect with the hollow pipeline；
The other end of the hollow pipeline is used to be connected with handle of a knife.
2. cell scraper cutter head according to claim 1, it is characterised in that length of much individual described holes along the edge of a knife
Degree direction is evenly arranged.
3. cell scraper cutter head according to claim 1, it is characterised in that for the connection handle in cone, it is thinner
One end and the blade are affixed.
4. cell scraper cutter head according to claim 1, it is characterised in that the edge of a knife is in yi word pattern or circular arc.
5. cell scraper cutter head according to claim 1, it is characterised in that the blade and the connection handle are using height
Density polyethylene material is integrally formed.
6. cell scraper cutter head according to claim 1, it is characterised in that the diameter of multiple described holes is 200um
~500um.
7. a kind of cell scraper, it is characterised in that the cell that the cell scraper is included as described in claim any one of 1-4 is scraped
Knife cutter head, in addition to handle of a knife；
Piston hole is set inside the handle of a knife, and the piston hole is internally provided with piston；
Described handle of a knife one end is fitted with piston rod, and the piston rod and the piston are affixed；The other end of the handle of a knife with it is described
The end connection of connection handle, and the piston hole connects with hollow pipeline sealing.
8. cell scraper according to claim 5, it is characterised in that connection handle described in the handle of a knife is detachably connected.
9. cell scraper according to claim 8, it is characterised in that the end set of the connection handle has slot, described
Handle of a knife is provided with plug, and the plug is inserted into the slot.
10. cell scraper according to claim 7, it is characterised in that the handle of a knife uses transparent material, along the piston
The length direction in hole is provided with scale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711384389.4A CN107841454A (en) | 2017-12-20 | 2017-12-20 | Cell scraper cutter head and cell scraper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711384389.4A CN107841454A (en) | 2017-12-20 | 2017-12-20 | Cell scraper cutter head and cell scraper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107841454A true CN107841454A (en) | 2018-03-27 |
Family
ID=61684271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711384389.4A Pending CN107841454A (en) | 2017-12-20 | 2017-12-20 | Cell scraper cutter head and cell scraper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107841454A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080161722A1 (en) * | 2006-12-29 | 2008-07-03 | Industrial Technology Research Institute | Aspiration device and method for cells and tissues |
| CN205391136U (en) * | 2016-02-28 | 2016-07-27 | 李正旺 | Tumour sampler |
| CN206266610U (en) * | 2016-11-18 | 2017-06-20 | 四川大学华西医院 | A kind of cell scraper |
| CN206420687U (en) * | 2017-01-09 | 2017-08-18 | 河南省肿瘤医院 | A kind of polypeptide chain mark sampling probe |
-
2017
- 2017-12-20 CN CN201711384389.4A patent/CN107841454A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080161722A1 (en) * | 2006-12-29 | 2008-07-03 | Industrial Technology Research Institute | Aspiration device and method for cells and tissues |
| CN205391136U (en) * | 2016-02-28 | 2016-07-27 | 李正旺 | Tumour sampler |
| CN206266610U (en) * | 2016-11-18 | 2017-06-20 | 四川大学华西医院 | A kind of cell scraper |
| CN206420687U (en) * | 2017-01-09 | 2017-08-18 | 河南省肿瘤医院 | A kind of polypeptide chain mark sampling probe |
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Effective date of registration: 20230428 Address after: 830000 Room 201, Building 1, No. 46 Satellite Road, Phase II, Urumqi Economic Development Zone (Toutunhe District), Xinjiang Uygur Autonomous Region Applicant after: Tiankang biopharmaceutical Co.,Ltd. Address before: 830000 No. 528 Changchun South Road, Urumqi high tech Industrial Development Zone, Xinjiang Uygur Autonomous Region Applicant before: TECON BIOLOGICAL Co.,Ltd. |
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