CN107837202A - A kind of re-surface intensive wrinkle correct breast containing microbial fermentation solution - Google Patents
A kind of re-surface intensive wrinkle correct breast containing microbial fermentation solution Download PDFInfo
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- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
- A61K8/894—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone modified by a polyoxyalkylene group, e.g. cetyl dimethicone copolyol
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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Abstract
The invention belongs to cosmetic technical field, and in particular to a kind of re-surface intensive wrinkle correct breast containing microbial fermentation solution.The present invention prepares microbial fermentation solution by bacillus amyloliquefaciens fermentation by extraction raw material of pigeon bone to extract polypeptide and/or amino acid, then the elite breast with crease-resistant function is formed with cosmetic, with crease-resistant, senile-resistant efficacy, the elite breast prepared is homogeneous；PH is stable between 6.2 7.8, and content of peptides is still more than the 98% of former content after 36 months, extends shelf life.
Description
Technical field
The invention belongs to cosmetic technical field, and in particular to a kind of re-surface intensive wrinkle correct breast containing microbial fermentation solution.
Background technology
Cosmetics are the methods to embrocate, spray or other are similar, intersperse among skin, hair, nail and the mouth of human body
The positions such as lip, to reach cleaning, removing bad smell, skin care, beauty and the daily chemical product for modifying purpose.State's alienation at present
Cosmetic is predominantly directed to functional cosmetics direction and developed, such as anti-wrinkle cosmetic；Another development finds to be exactly to make up
The raw material of product is replaced by more green, safe natural materials or natural fermented, extraction of substance by traditional industrial chemicals.
By microbial fermentation engineering and the active component that obtains, contain and broken without breaking-wall cell processing, by cell
The active component of wall processing, is referred to as Microbial fermentation extracts.Such as yeast tunning extract makes in cosmetics
With frequent, its effect is also generally satisfactory.In terms of skin senescence is resisted, yeast extractive from fermentative is to skin regeneration and glue
Former albumen synthesis has good facilitation.Also report in the prior art and feature is largely obtained by biofermentation
The case of cosmetic.Such as system of the lactobacillus-fermented rice extract solution as skin care item active component disclosed in US8071115B2
Method is made, hair quality, bright color can be changed by describing addition lactobacillus-fermented rice extract solution in skin care item, and can be made
Cosmetic emulsion is more stable.Disclosed in US5888521 and a kind of include hydroxy acid and oat extract, and it was found that it is this into
Cytothesis can be promoted by dividing；Disclosed in US5324515 in a kind of product from lactobacillus-fermented and obtain cosmetic raw materials
Method, for preparing has antioxidation containing lactobacillus cell lysate, and can repair the DNA of skin damage,
Improve the immunity of skin.A kind of oat zymotic fluid is disclosed in CN105296541A and is used as cosmetic material, oat is entered
Oat zymotic fluid is made in row fermentative bioconversion, can have the nutrition of probiotics and vegetable active thing concurrently, have excellent anti-aging,
Antioxidation.
Pigeon soup is one of vegetable, China's saying among the people for having " dove wins nine chickens ".Modern medicine is thought：Pigeon meat is strengthened body and mended
Kidney, life vigor, brain tonic mend god, improve memory, reduce blood pressure, adjustment blood sugar for human body, skin maintenance, the pure white delicacy of skin,
Promote longevity and other effects.But what people ate in pigeon soup is mostly pigeon meat and the collagen endured out of part, containing in pigeon bone
There is substantial amounts of collagen not to be fully utilized.Up to now pigeon bone is extracted into collagen egg by fermenting without document report
In vain, polypeptide and amino acid prepare the cosmetics of crease-resistant function.
The content of the invention
It is an object of the invention to provide a kind of novel microbial zymotic fluid to prepare the elite breast with crease-resistant function, this hair
It is bright to prepare one kind to extract polypeptide and/or amino acid by microbial fermentation using pigeon bone as extraction raw material and there is crease-resistant function
Elite breast, there is crease-resistant, senile-resistant efficacy, the elite breast prepared is homogeneous；PH is stable between 6.2-7.8, after 36 months
Content of peptides is still more than the 98% of former content, extends shelf life.
According to an aspect of the present invention, the invention provides a kind of re-surface intensive wrinkle correct breast containing microbial fermentation solution, by weight
Measure number meter：By microbial fermentation solution 8-12 parts, acrylic acid/stearyl alcohol acrylate copolymer 0.4-0.6 parts, adipic acid/bis-
Poly- linoleic acid/hexylene glycol copolymer 0.2-0.4 parts, apricot kernel oil amido propyl benzyl chloride 1.1-1.3 parts, aloe are bitter
Plain 0.03-0.05 parts, 5- amino -2,6- dimethoxy -3- Hydroxy-pyridine 0.06-0.08 parts, myristyl alcohol polyethers sulfuric ester
Ammonium 0.2-0.3 parts, glycerine 7.0-9.0 parts, double-isobutyl group PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymers 0.3-0.5
Part, hydrolysis Jojoba albumen 0.1-0.3 parts, stabilizer 0.2-0.5 parts, deionized water 85-90 parts；
The microbial fermentation solution prepares gained by following preparation method：
1) pigeon bone pre-treatment：PH is used to soak 30-60min for 8.2-9.0 weak base aqueous solution after pigeon bone is cleaned,
Then filter, wash to filtrate pH into neutrality, be placed under 0.5MPa water vapour pressure after carrying out boiling heat treatment 1h and dry, so
Crush afterwards, 400-600 mesh sieves excessively obtain pigeon bone meal；
2) preparation of seed bacterium solution：By bacillus amyloliquefaciens 1.0g be placed in the fluid nutrient mediums of 50ml first in 35 DEG C,
100rpm concussion and cultivates 24h obtains seed bacterium solution；First fluid nutrient medium is the suspension of water, is contained in every 1000ml suspensions
Tryptone 12g, yeast extract 1.3g, agar 8.1g, pigeon bone meal 0.4g, maltose 1.3g, lithium chloride 0.4g, cesium acetate
0.05g；
3) one grade fermemtation culture：Seed bacterium solution is inoculated in fermentation medium and cultivated, inoculum concentration 8%wt, control
Ventilation 0.5vvm processed, mixing speed 100rpm, in 6.5-7.5, temperature is 30 DEG C for pH value control, and one-level is obtained after cultivating 36h
Zymotic fluid；
4) second order fermentation culture：One grade fermemtation liquid is inoculated in fermentation medium and cultivated, inoculum concentration 14%wt,
Ventilation 0.6vvm is controlled, mixing speed 120rpm, in 7.2-7.5, temperature is 30 DEG C for pH value control, and two are obtained after cultivating 24h
Level zymotic fluid；
5) fermented and cultured：Second order fermentation liquid is inoculated in fermentation medium and cultivated, inoculum concentration 12%wt, control
Ventilation 0.65vvm, mixing speed 100rpm, in 7.2-7.5, temperature is 35 DEG C for pH value control, and primary hair is obtained after cultivating 24h
Zymotic fluid；Primary fermentation liquid is filtered, and then controls ventilation 0.8vvm, mixing speed 100rpm, and pH value is controlled in 7.2-
7.5, temperature is 35 DEG C, and final zymotic fluid is obtained after cultivating 24h；
6) post-process：Final zymotic fluid is centrifuged into obtain centrifugate, at 10 DEG C after then centrifugate is mixed with water for injection
Down by the filtering with microporous membrane that aperture is 0.15 micron, microbial fermentation solution is obtained after being heat-treated 10s finally by water vapour；Its
The weight ratio of middle centrifugate weight and water for injection is 1:3；
The fermentation medium is the suspension of water, per 1000ml suspensions in 15g containing tryptone, yeast extract 1.5g,
Agar 6.8g, pigeon bone meal 5.8g, maltose 3.3g, lithium chloride 0.2g, cesium acetate 0.15g, wheat germ powder 4.6g；Wheat
Bud powder is as compound nitrogen source, and maltose is as carbon source；Lithium chloride and cesium acetate can improve fermentation efficiency (research initial screening
Manganese chloride, zinc chloride, iron chloride, calcium chloride, magnesium chloride all have certain inhibitory action to bacterium vigor).
The stabilizer is 1,2-diaminocyclohexane tetraacetic acid, preferably aminoethane sulfinic acid, aminoethane sulfinic acid；The present invention
The raw materials used microbial fermentation solution of the breast of the re-surface intensive wrinkle correct containing microbial fermentation solution prepared contains albumen, short chain polypeptides and amino
Acid, these compositions, which play, resists and protects skin because heat, free radical, heavy metal damage to caused by Skin Cell, so as to
Play delay skin aging, crease-resistant effect；But because albumen, polypeptide can be slowly degraded into shelf life due to placing for a long time
Small molecule amino acid, cause elite breast pH reductions, on the one hand can produce the side effect for stimulating skin, on the other hand can reduce elite
The effect of breast is crease-resistant, anti-aging；The present invention screening phosphate buffer salt (neutral buffered scope), 4- hydroxyethyl piperazineethanesulfonic acids,
The plurality of stable agent such as 1,2-diaminocyclohexane tetraacetic acid, aminoethane sulfinic acid, finally select 1,2-diaminocyclohexane tetraacetic acid, aminoethane Asia sulphur
Acid is used as the buffer in system of the present invention, can maintain under lucifuge elite breast pH stably between 6.2-7.8 in 24 months.
Re-surface intensive wrinkle correct breast of the present invention containing microbial fermentation solution, is counted in parts by weight：Preferably, sent out by microorganism
10 parts of zymotic fluid, acrylic acid/0.5 part of stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer 0.3
Part, 1.2 parts of apricot kernel oil amido propyl benzyl chloride, 0.04 part of Aloesin, 5- amino -2,6- dimethoxys -3-
0.07 part of Hydroxy-pyridine, 0.3 part of myristyl alcohol polyethers sulfuric ester ammonium, 8.0 parts of glycerine, double-isobutyl group PEG-24/PPG-7/ gather
88 parts of 0.4 part of dimethylsiloxane copolymer, 0.2 part of hydrolysis Jojoba albumen, 0.4 part of stabilizer, deionized water compositions.
According to another aspect of the present invention, the invention provides a kind of system of the re-surface intensive wrinkle correct breast containing microbial fermentation solution
Preparation Method, comprise the following steps：
1) by acrylic acid/stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer, apricot kernel oil
Amido propyl benzyl chloride, Aloesin, 5- amino -2,6- dimethoxy -3- Hydroxy-pyridines, myristyl alcohol gather
Ether sulfuric ester ammonium and glycerine stir, and are heated to 140 DEG C, under nitrogen atmosphere under 1500rpm homogeneous 2h, then cool
The first mixed liquor is obtained to 80 DEG C；
2) will double-isobutyl group PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymers, hydrolysis Jojoba albumen and deionization
Water be heated to 85 DEG C under 2000rpm homogeneous 24h obtain the second mixed liquor；
3) the first mixed liquor is well mixed with the second mixed liquor, then adds stabilizer, take gradient cooling
Mode carries out ultrasonic homogeneous and obtains the 3rd mixed liquor；
4) add microbial fermentation solution into the 3rd mixed liquor, room temperature is cooled to after homogeneous 24h at 30 DEG C, under 1200rpm
The re-surface intensive wrinkle correct breast of microbial fermentation solution must be contained.
Step 3) is described to be taken the mode of gradient cooling to carry out ultrasonic homogeneous to refer to that temperature maintains 80 DEG C, under 1000rpm
Ultrasonic homogeneous 30min, 70 DEG C, ultrasonic homogeneous 30min under 1000rpm, 60 DEG C, ultrasonic homogeneous 30min under 1000rpm；50℃、
Homogeneous 30min under 1000rpm, 40 DEG C, homogeneous 24h under 1000rpm；Due to containing a large amount of copolymers, such as propylene in elite breast
Acid/stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer, double-isobutyl group PEG-24/PPG-7/
Polydimethylsiloxanecopolymer copolymer low temperature in system is not uniformly dispersed, and high temperature (referring to more than more than 100 DEG C) and easily makes copolymerization
Thing depolymerization itself is either copolymerized with other copolymers or small molecule causes glomeration to be more difficult to scatter；The present invention is super
Gradient homogeneous is carried out in the case of sound and solves the problems, such as the scattered uneven or glomeration of copolymer, the breast prepared well
Liquid is homogeneous；And accumulation situation will not occur for placement for a long time.
The present invention obtains the microorganism containing albumen, polypeptide, amino acid by microbial fermentation as raw material using pigeon bone and sent out
Zymotic fluid prepares a kind of new re-surface intensive wrinkle correct breast, not only physicochemical property stable homogeneous in 24 months, and with well it is crease-resistant,
Anti-senescence function.
Compared with prior art, the invention has the advantages that：
1) zymotic fluid of albumen, polypeptide, amino acid is prepared by microbial fermentation the invention provides a kind of pigeon bone
Method, turn waste into wealth and improve the utilization rate of pigeon bone；
2) zymotic fluid and a variety of cosmetic materials are prepared into elite breast by the present invention, have crease-resistant, senile-resistant efficacy, and
Ultrasonic homogeneous manner is carried out by way of taking gradient cooling and solves copolymer depolymerization, phenomenon of accumulation in elite breast,
Make the elite breast prepared more homogeneous；
3) present invention makes elite breast pH stable between 6.2-7.8 by screening stabilizer, and content of peptides is still after 36 months
For more than the 96.3% of former content, shelf life is extended.
Embodiment
To make the object, technical solutions and advantages of the present invention of greater clarity, with reference to embodiment, to this
Invention is further described.It should be understood that these descriptions are merely illustrative, and it is not intended to limit the scope of the present invention.
Acrylic acid/stearyl alcohol acrylate copolymer comes from Angene companies of the U.S., and No. CAS is 36120-03-3；Oneself two
Acid/dimerized linoleic acid/hexylene glycol copolymer CAS is 171542-56-6；Apricot kernel oil amido propyl benzyl chloride
No. CAS is 124046-03-3；Myristyl alcohol polyethers sulfuric ester ammonium CAS is 27731-61-9；Double-isobutyl group PEG-24/
PPG-7/ polydimethylsiloxanecopolymer copolymers CAS are 158451-77-5；Hydrolyze Jojoba albumen No. CAS is 100684-
35-3；Bacillus amyloliquefaciens come from Jiangsu Lv Ke Bioisystech Co., Ltd commercially produced product, and viable bacteria content >=200 ×
108CFU/g；In embodiment in addition to without specified otherwise, all materials are measured with parts by weight；Content of peptides test reference
In the prior art (Food Science, 2005,26:Teaching 7P169-171) is completed；The fermentation medium that embodiment uses is water
Suspension, per 1000ml suspensions in 15g containing tryptone, yeast extract 1.5g, agar 6.8g, pigeon bone meal 5.8g, malt
Sugared 3.3g, lithium chloride 0.2g, cesium acetate 0.15g, wheat germ powder 4.6g.
Embodiment 1
Prepare microbial fermentation solution：
1) pigeon bone pre-treatment：By pigeon bone clean after use pH for 8.5 potassium hydroxide base aqueous solution soaking 30-
60min, then filter, wash to filtrate pH into neutrality, be placed under 0.5MPa water vapour pressure after carrying out boiling heat treatment 1h
Drying, then crush, 400-600 mesh sieves excessively obtain pigeon bone meal；
2) preparation of seed bacterium solution：By bacillus amyloliquefaciens 1.0g be placed in the fluid nutrient mediums of 50ml first in 35 DEG C,
100rpm concussion and cultivates 24h obtains seed bacterium solution；First fluid nutrient medium is the suspension of water, egg containing pancreas in every 1000ml suspensions
White peptone 12g, yeast extract 1.3g, agar 8.1g, pigeon bone meal 0.4g, maltose 1.3g, lithium chloride 0.4g, cesium acetate 0.05g；
3) one grade fermemtation culture：Seed bacterium solution is inoculated in fermentation medium and cultivated, inoculum concentration 8%wt, control
Ventilation 0.5vvm processed, mixing speed 100rpm, in 6.5-7.5, temperature is 30 DEG C for pH value control, and one-level is obtained after cultivating 36h
Zymotic fluid；
4) second order fermentation culture：One grade fermemtation liquid is inoculated in fermentation medium and cultivated, inoculum concentration 14%wt,
Ventilation 0.6vvm is controlled, mixing speed 120rpm, in 7.2-7.5, temperature is 30 DEG C for pH value control, and two are obtained after cultivating 24h
Level zymotic fluid；
5) fermented and cultured：Second order fermentation liquid is inoculated in fermentation medium and cultivated, inoculum concentration 12%wt, control
Ventilation 0.65vvm, mixing speed 100rpm, in 7.2-7.5, temperature is 35 DEG C for pH value control, and primary hair is obtained after cultivating 24h
Zymotic fluid；Primary fermentation liquid is filtered, and then controls ventilation 0.8vvm, mixing speed 100rpm, and pH value is controlled in 7.2-
7.5, temperature is 35 DEG C, and final zymotic fluid is obtained after cultivating 24h；
6) post-process：Final zymotic fluid is centrifuged into obtain centrifugate, at 10 DEG C after then centrifugate is mixed with water for injection
Down by the filtering with microporous membrane that aperture is 0.15 micron, microbial fermentation solution is obtained after being heat-treated 10s finally by water vapour；Its
The weight ratio of middle centrifugate weight and water for injection is 1:3.
Prepare re-surface intensive wrinkle correct breast：
1) by acrylic acid/0.4 part of stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer
0.2 part, 1.1 parts of apricot kernel oil amido propyl benzyl chloride, 0.03 part of Aloesin, 5- amino -2,6- dimethoxies
7.0 parts of 0.06 part of base -3- Hydroxy-pyridines, 0.2 part of myristyl alcohol polyethers sulfuric ester ammonium and glycerine stir, and are heated to
140 DEG C, under nitrogen atmosphere under 1500rpm homogeneous 2h, be then cooled to 80 DEG C the first mixed liquor；
2) will double -0.3 part of isobutyl group PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymers, hydrolysis Jojoba albumen 0.1
Part and deionized water 85-90 parts be heated to 85 DEG C under 2000rpm homogeneous 24h obtain the second mixed liquor；
3) the first mixed liquor is well mixed with the second mixed liquor, then adds stabilizer 1,2-diaminocyclohexane tetraacetic acid
0.2 part, take the mode of gradient cooling to carry out ultrasonic homogeneous and obtain the 3rd mixed liquor；Step 3) the side for taking gradient cooling
Formula carry out ultrasonic homogeneous refer to temperature maintain 80 DEG C, ultrasonic homogeneous 30min under 1000rpm, 70 DEG C, ultrasound is under 1000rpm
Matter 30min, 60 DEG C, ultrasonic homogeneous 30min under 1000rpm；50 DEG C, homogeneous 30min under 1000rpm, 40 DEG C, under 1000rpm
Matter 24h；
4) 8 parts of microbial fermentation solution is added into the 3rd mixed liquor, room is cooled to after homogeneous 24h at 30 DEG C, under 1200rpm
The re-surface intensive wrinkle correct breast of Wen get Han microbial fermentation solutions.
It is 7.02 that re-surface intensive wrinkle correct breast, which prepares initial pH when completing, and pH is 6.62 after being preserved 3 years after the sealing of normal temperature lucifuge, more
Peptide total content is the 89.2% of initial content.
Embodiment 2
Microbial fermentation liquid and preparation method thereof is the same as embodiment 1.
Prepare re-surface intensive wrinkle correct breast：
1) by acrylic acid/0.5 part of stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer
0.3 part, 1.2 parts of apricot kernel oil amido propyl benzyl chloride, 0.04 part of Aloesin, 5- amino -2,6- dimethoxies
8.0 parts of 0.07 part of base -3- Hydroxy-pyridines, 0.3 part of myristyl alcohol polyethers sulfuric ester ammonium and glycerine stir, and are heated to
140 DEG C, under nitrogen atmosphere under 1500rpm homogeneous 2h, be then cooled to 80 DEG C the first mixed liquor；
2) will double -0.4 part of isobutyl group PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymers, hydrolysis Jojoba albumen 0.2
Part and 88 parts of deionized water be heated to 85 DEG C under 2000rpm homogeneous 24h obtain the second mixed liquor；
3) the first mixed liquor is well mixed with the second mixed liquor, then adds stabilizer aminoethane sulfinic acid
0.3 part, take the mode of gradient cooling to carry out ultrasonic homogeneous and obtain the 3rd mixed liquor；Step 3) the side for taking gradient cooling
Formula carry out ultrasonic homogeneous refer to temperature maintain 80 DEG C, ultrasonic homogeneous 30min under 1000rpm, 70 DEG C, ultrasound is under 1000rpm
Matter 30min, 60 DEG C, ultrasonic homogeneous 30min under 1000rpm；50 DEG C, homogeneous 30min under 1000rpm, 40 DEG C, under 1000rpm
Homogeneous 24h；
4) microbial fermentation solution 8-12 parts are added into the 3rd mixed liquor, are cooled at 30 DEG C, under 1200rpm after homogeneous 24h
The re-surface intensive wrinkle correct breast of microbial fermentation solution must be contained to room temperature.
It is 7.13 that re-surface intensive wrinkle correct breast, which prepares initial pH when completing, and it is homogeneous to visually observe elite breast matter amount, and no particulate matter is reunited
Phenomenon；PH is 6.98 after being preserved 3 years after the sealing of normal temperature lucifuge, and polypeptide total content is the 98.4% of initial content, and without particle
Thing accumulation phenomena occurs.
If 80 DEG C are taken in the preparation process of step 3), homogeneous 24h and then is cooled to 40 DEG C of then later stages under 1000rpm
The elite breast prepared the accumulation phenomena of particulate matter will occur after placing 12 months.
Embodiment 3
Microbial fermentation liquid and preparation method thereof is the same as embodiment 1.
Prepare re-surface intensive wrinkle correct breast：
1) by acrylic acid/0.6 part of stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer
0.4 part, 1.3 parts of apricot kernel oil amido propyl benzyl chloride, 0.05 part of Aloesin, 5- amino -2,6- dimethoxies
9.0 parts of 0.08 part of base -3- Hydroxy-pyridines, 0.3 part of myristyl alcohol polyethers sulfuric ester ammonium and glycerine stir, and are heated to
140 DEG C, under nitrogen atmosphere under 1500rpm homogeneous 2h, be then cooled to 80 DEG C the first mixed liquor；
2) will double -0.5 part of isobutyl group PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymers, hydrolysis Jojoba albumen 0.3
Part and 90 parts of deionized water be heated to 85 DEG C under 2000rpm homogeneous 24h obtain the second mixed liquor；
3) the first mixed liquor is well mixed with the second mixed liquor, then adds stabilizer aminoethane sulfinic acid
0.5 part, take the mode of gradient cooling to carry out ultrasonic homogeneous and obtain the 3rd mixed liquor；
4) 12 parts of microbial fermentation solution is added into the 3rd mixed liquor, is cooled at 30 DEG C, under 1200rpm after homogeneous 24h
Room temperature must contain the re-surface intensive wrinkle correct breast of microbial fermentation solution.
It is 7.26 that re-surface intensive wrinkle correct breast, which prepares initial pH when completing, and it is homogeneous to visually observe elite breast matter amount, and no particulate matter is reunited
Phenomenon；PH is 6.88 after being preserved 3 years after the sealing of normal temperature lucifuge, and polypeptide total content is the 88.4% of initial content, and without particle
Thing accumulation phenomena occurs.
Comparative example
Using pH changes and polypeptide degradation as index, using single-factor variable method research stabilizer, (remaining raw material and operation are same
Embodiment 2 is completely the same) to re-surface intensive wrinkle correct breast stabilization, as a result as shown in table 1：
Stabilization of the different stabilizers of table 1 to re-surface intensive wrinkle correct breast
Above result of the test shows that stabilizer 1,2-diaminocyclohexane tetraacetic acid, the aminoethane sulfinic acid that the present invention uses are excellent
In traditional PB and biological buffer 4- hydroxyethyl piperazineethanesulfonic acids.
The re-surface intensive wrinkle correct breast prepared in beauty parlor using the present invention carries out crease-resistant test evaluation, is using wrinkle of skin test
Instrument (German CKVL650 types) is tested the embodiment 1-3 re-surface intensive wrinkle correct breasts prepared, and anti-wrinkle effect evaluation is divided into 4 grades：(1 grade
Cure, wrinkle scar disappears；2 grades effective, and wrinkle substantially weakens；3 grades effectively, wrinkle weakened at different degrees；4 grades of invalid, wrinkles
It is without any improvement or have exacerbation sign).
Subjects：Healthy women, one full year of life at age 40-45, test period are 3 months, record the situation of different time.
The anti-wrinkle effect evaluation table of table 2
Note：Microbial fermentation solution is not added to refer to compared with Example 2, does not add microbial fermentation prepared by the present invention
Liquid, remaining is completely the same with embodiment 2.
Although embodiments of the present invention are described in detail, it should be understood that, without departing from the present invention's
In the case of spirit and scope, embodiments of the present invention can be made with various changes, replacement and change.
Claims (8)
1. a kind of re-surface intensive wrinkle correct breast containing microbial fermentation solution, is counted in parts by weight：By microbial fermentation solution 8-12 parts, propylene
Acid/stearyl alcohol acrylate copolymer 0.4-0.6 parts, adipic acid/dimerized linoleic acid/hexylene glycol copolymer 0.2-0.4 parts, almond
Oleamide benzyl alkyl dimethyl ammonium chloride 1.1-1.3 parts, Aloesin 0.03-0.05 parts, 5- amino -2,6- dimethoxys -
3- Hydroxy-pyridine 0.06-0.08 parts, myristyl alcohol polyethers sulfuric ester ammonium 0.2-0.3 parts, glycerine 7.0-9.0 parts, double-isobutyl group
PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymer 0.3-0.5 parts, hydrolysis Jojoba albumen 0.1-0.3 parts, stabilizer 0.2-
0.5 part, deionized water 85-90 parts composition；
The microbial fermentation solution prepares gained by following preparation method：
Pigeon bone pre-treatment：PH is used to soak 30-60min for 8.2-9.0 weak base aqueous solution after pigeon bone is cleaned, then mistake
Filter, wash to filtrate pH into neutrality, be placed under 0.5MPa water vapour pressure after carrying out boiling heat treatment 1h and dry, then powder
Broken, 400-600 mesh sieves excessively obtain pigeon bone meal；
The preparation of seed bacterium solution：Bacillus amyloliquefaciens 1.0g is placed in the fluid nutrient mediums of 50ml first in 35 DEG C, 100rpm
Concussion and cultivate 24h obtains seed bacterium solution；First fluid nutrient medium is the suspension of water, contains tryptose in every 1000ml suspensions
Peptone 12g, yeast extract 1.3g, agar 8.1g, pigeon bone meal 0.4g, maltose 1.3g, lithium chloride 0.4g, cesium acetate 0.05g；
One grade fermemtation culture：Seed bacterium solution is inoculated in fermentation medium and cultivated, inoculum concentration 8%wt, controls ventilation
0.5vvm, mixing speed 100rpm, in 6.5-7.5, temperature is 30 DEG C for pH value control, and one grade fermemtation liquid is obtained after cultivating 36h；
Second order fermentation culture：One grade fermemtation liquid is inoculated in fermentation medium and cultivated, inoculum concentration 14%wt, control is led to
Air quantity 0.6vvm, mixing speed 120rpm, in 7.2-7.5, temperature is 30 DEG C for pH value control, and second order fermentation is obtained after cultivating 24h
Liquid；
Fermented and cultured：Second order fermentation liquid is inoculated in fermentation medium and cultivated, inoculum concentration 12%wt, controls ventilation
0.65vvm, mixing speed 100rpm, in 7.2-7.5, temperature is 35 DEG C for pH value control, and primary fermentation liquid is obtained after cultivating 24h；
Primary fermentation liquid is filtered, and then controls ventilation 0.8vvm, mixing speed 100rpm, pH value control is in 7.2-7.5, temperature
Spend for 35 DEG C, final zymotic fluid is obtained after cultivating 24h；
Post processing：Final zymotic fluid is centrifuged into obtain centrifugate, passed through after then centrifugate is mixed with water for injection at 10 DEG C
Aperture is 0.15 micron of filtering with microporous membrane, and microbial fermentation solution is obtained after being heat-treated 10s finally by water vapour；Wherein centrifuge
Liquid weight and the weight of water for injection ratio are 1:3.
2. the re-surface intensive wrinkle correct breast according to claim 1 containing microbial fermentation solution, it is characterised in that：Count in parts by weight：
By 10 parts of microbial fermentation solution, acrylic acid/0.5 part of stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol
0.3 part of copolymer, 1.2 parts of apricot kernel oil amido propyl benzyl chloride, 0.04 part of Aloesin, 5- amino -2,6- two
0.07 part of methoxyl group -3- Hydroxy-pyridines, 0.3 part of myristyl alcohol polyethers sulfuric ester ammonium, 8.0 parts of glycerine, double-isobutyl group PEG-24/
0.4 part of PPG-7/ polydimethylsiloxanecopolymer copolymers, 0.2 part of hydrolysis Jojoba albumen, 0.4 part of stabilizer, 88 parts of deionized water
Composition.
3. the re-surface intensive wrinkle correct breast according to claim 1 containing microbial fermentation solution, it is characterised in that：The fermentation medium
For the suspension of water, per 1000ml suspensions in 15g containing tryptone, yeast extract 1.5g, agar 6.8g, pigeon bone meal 5.8g,
Maltose 3.3g, lithium chloride 0.2g, cesium acetate 0.15g, wheat germ powder 4.6g.
4. the re-surface intensive wrinkle correct breast according to claim 1 containing microbial fermentation solution, it is characterised in that：The stabilizer is ring
Hexamethylene tetraacetic acid or aminoethane sulfinic acid.
5. the re-surface intensive wrinkle correct breast according to claim 1 containing microbial fermentation solution, it is characterised in that：The stabilizer is ammonia
Base ethane sulfinic acid.
6. the re-surface intensive wrinkle correct breast according to claim 1 containing microbial fermentation solution, it is characterised in that：Elite breast pH is 6.2-
7.8。
7. a kind of preparation method of the re-surface intensive wrinkle correct breast containing microbial fermentation solution, comprises the following steps：
By acrylic acid/stearyl alcohol acrylate copolymer, adipic acid/dimerized linoleic acid/hexylene glycol copolymer, almond oleamide third
Base benzyl chloride, Aloesin, 5- amino -2,6- dimethoxy -3- Hydroxy-pyridines, myristyl alcohol polyethers sulfuric acid
Ester ammonium and glycerine stir, and are heated to 140 DEG C, under nitrogen atmosphere under 1500rpm homogeneous 2h, be then cooled to 80 DEG C
Obtain the first mixed liquor；
Will double-isobutyl group PEG-24/PPG-7/ polydimethylsiloxanecopolymer copolymers, hydrolysis Jojoba albumen and deionized water heating
To 85 DEG C under 2000rpm homogeneous 24h obtain the second mixed liquor；
First mixed liquor is well mixed with the second mixed liquor, stabilizer is then added, takes the mode of gradient cooling to enter
The ultrasonic homogeneous of row obtains the 3rd mixed liquor；
Add microbial fermentation solution into the 3rd mixed liquor, be cooled at 30 DEG C, under 1200rpm after homogeneous 24h room temperature must contain it is micro-
The re-surface intensive wrinkle correct breast of bio-fermented liquid.
8. preparation method according to claim 7, it is characterised in that：Step 3）The mode for taking gradient cooling is carried out
Ultrasonic homogeneous refer to temperature maintain 80 DEG C, ultrasonic homogeneous 30min under 1000rpm, 70 DEG C, ultrasonic homogeneous under 1000rpm
30min, 60 DEG C, ultrasonic homogeneous 30min under 1000rpm；50 DEG C, ultrasonic homogeneous 30min under 1000rpm, 40 DEG C, under 1000rpm
Ultrasonic homogeneous 24h.
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| CN115867250A (en) * | 2020-07-31 | 2023-03-28 | 莱雅公司 | Skin tightening cosmetic compositions and methods of use |
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| CN115867250A (en) * | 2020-07-31 | 2023-03-28 | 莱雅公司 | Skin tightening cosmetic compositions and methods of use |
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Application publication date: 20180327 |