CN107828770A - It is a kind of to solidify organophosphor hydrolytic enzyme method in non-woven fabrics glazing - Google Patents
It is a kind of to solidify organophosphor hydrolytic enzyme method in non-woven fabrics glazing Download PDFInfo
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- CN107828770A CN107828770A CN201711040182.5A CN201711040182A CN107828770A CN 107828770 A CN107828770 A CN 107828770A CN 201711040182 A CN201711040182 A CN 201711040182A CN 107828770 A CN107828770 A CN 107828770A
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- woven fabrics
- hydrolytic enzyme
- organophosphor hydrolytic
- enzyme
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D31/00—Materials specially adapted for outerwear
- A41D31/04—Materials specially adapted for outerwear characterised by special function or use
-
- A—HUMAN NECESSITIES
- A42—HEADWEAR
- A42B—HATS; HEAD COVERINGS
- A42B1/00—Hats; Caps; Hoods
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Textile Engineering (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
Abstract
The invention provides a kind of method for fixing organophosphor hydrolytic enzyme on non-woven fabrics using photocuring technology, methods described comprises the following steps：（1）The blended liquid of water soluble photo-curable resin system and organophosphor hydrolytic enzyme is configured, light-cured resin system is made up of water-soluble acrylic ester performed polymer and light trigger；（2）The blended liquid configured is coated on non-woven fabrics, solidified under ultraviolet or visible-light curing light source；（3）Non-woven fabrics after will be immobilized is dried, the non-woven fabrics for having prepared organophosphor hydrolytic enzyme immobilized.Immobilized organophosphor hydrolytic enzyme is prepared using this method, not only enzyme activity operational stability and bin stability significantly improve, and easy to use, can make the fabrics such as mouth mask.
Description
Technical field
The present invention relates to solidification zymotechnic field, a kind of non-woven fabrics fabric of immobilized organophosphor hydrolytic enzyme of specific design and its
In the application of civilian or military biochemical protective mask and protective garment.
Background technology
Organic phosphorus compound is a kind of chemical reagent with neurotoxicity, because its species is more, toxicity is high, persistence is low in environment
The features such as and substitute organochlorine as the most widely used a kind of agricultural chemicals in agricultural production, not only usage amount is doubled and redoubled,
Use range also gradually expands, and expands to bactericide, invertebrate poison from the insecticide of control diseases and pests of agronomic crop, kills mouse
The purposes such as agent, herbicide, defoliant and plant growth regulator.In addition, the mankind also utilize the specific toxicity of organic phosphorus compound
To develop chemical weapons, such as sarin (methyl fluophosphonic acid isopropyl ester), soman (the different ethyl ester of BFPO acid) and VX (O- ethyl-S- [2-
(diisopropylaminoethyl) ethyl] methylphosphorothioate.Then, increasing organophosphate nerve agent enter air, soil,
In underground water and animal and plant body, huge harm is formd to the ecological balance and human health.
The degraded of organic phosphorus compound mainly passes through light degradation, chemical degradation and microbial degradation, light in nature
Degrade and chemical degradation efficiency is low and can produce secondary pollution.Compared with light degradation and chemical degradation, Biodegradation product is not
Secondary pollution can be produced, and has the advantages that atopic is high, reaction condition is gentle.Organophosphor hydrolytic enzyme is that one kind will be organic
Phosphorus compound hydrolyzes the enzyme of organic phosphorus compound as substrate, be it is a kind of be widely present in a variety of organisms by organophosphor
The esterase of hydrolase gene (opd) coding.Substrate of the organophosphor hydrolytic enzyme using organic phosphorus compound as enzymatic reaction, can be single-minded
Its phosphorus cyanogen, phosphorus sulphur, phosphorus fluorine and phosphorus oxygen key is cut off to property, so that it loses toxicity.
The essence of enzyme is protein, and protein has a limitation of its own, for example, easily by temperature, acid-base value, have
The influence of solvent and deactivation, and the catalytic life of resolvase is short, preserve that difficulty is big, and these shortcomings also greatly limit
Application of the resolvase in real life.Enzyme immobilization technology is mutually to tie water miscible resolvase with carrier not soluble in water
Close, enzyme derivative not soluble in water is made.Immobilised enzymes is real in wide spectrums such as food, medical treatment, the energy, environmental improvements at present
Certain application is showed.Enzyme immobilizatio method mainly has absorption method, embedding hair and chemical bonding processes.Physisorphtion causes enzyme
Combination between carrier is not that very firmly enzyme is easily come off from carrier, and with the increase of repeat usage, Physical is fixed
The enzymatic efficiency of change can be reduced substantially.Chemical bonding processes need to introduce active group, this kind of active group on enzyme and carrier
Enzyme can be made to be connected with carrier by covalent bond.Although chemical method immobilised enzymes so that the connection of enzyme and carrier is more firm,
But the formation of covalent bond is generally carried out in the case where wanting the harsh conditions such as high temperature, strong acid and strong base, some reactions in addition are needed organic
Reacted in solvent, which also limits the application of this method.Investment is that enzyme is carried out into embedding solidification using the crosslinking of polymer,
Because belong to physical method thus during immobilized to damage it is smaller, but still have two subject matters：It is first, conventional poly-
After compound embedding, enzyme is had most of enzyme not play a role by polymer barrier；Second, it can not be knitted after embedding in non-woven fabrics etc.
It is fixed on thing, it can only use in the solution.
The content of the invention
It is above-mentioned existing in order to solve the problems, such as, it is an object of the invention to provide a kind of photo-immobilization zymotechnic, pass through light
The immobilized embedding on non-woven fabrics fabric by organophosphor hydrolytic enzyme of the means of solidification.
In order to reach foregoing invention purpose, the present invention provides following technical scheme：
A kind of method for fixing organophosphor hydrolytic enzyme on non-woven fabrics using photocuring technology, it is characterised in that methods described bag
Include following steps：
(1) blended liquid of water soluble photo-curable resin system and organophosphor hydrolytic enzyme is configured, light-cured resin system is by water solubility
Acrylate prepolymer body and light trigger composition；
(2) blended liquid configured is coated on non-woven fabrics, solidified under ultraviolet or visible-light curing light source；
(3) non-woven fabrics after will be immobilized is dried.
Water-soluble acrylic ester performed polymer is polyethylene glycol acrylate, polyethylene glycol diacrylate in the step (1)
Ester, polyethylene glycol methacrylate-styrene polymer, polyethylene glycol dimethacrylate, ethoxyquin epoxy acrylate, ethoxyquin epoxy
In methacrylate, ethoxyquin trimethylolpropane trimethacrylate, ethoxyquin trimethylol-propane trimethacrylate
One or more mixtures.
Light trigger is selected from described in the step (1)：1173rd, 651,184, TPO, ITX, 819,784, benzophenone,
One or more in diaryl diazo salt diaryl group iodized salt, triaryl sulfonium salts, luxuriant molysite.
Photocuring light source is 300-532nm selected from high-pressure sodium lamp, Halogen lamp LED, dysprosium lamp and wavelength described in the step (2)
LED light source.
It is a kind of to adopt the non-woven fabrics for being loaded with organophosphor hydrolytic enzyme prepared with the aforedescribed process.It is loaded with the nothing of organophosphor hydrolytic enzyme
Spin purposes of the cloth in mouth mask, handkerchief, clothes, cap textile is prepared.
The present invention has the following advantages that relative to prior art：
(1) immobilized zymotechnic of the present invention is the immobilized zymotechnic of photocuring, the technical operation simple and fast, the bar of reaction
Part is gentleer, and reaction can be carried out in aqueous, and the speed of light reaction, and reaction can control, prior
It is that light source used inactivates enzyme.
(2) thus photosensitive resin solidification after there is macroporous structure, solve what investment stop portions enzyme played a role
Problem.
(3) organophosphor hydrolytic enzyme can be immobilized on by the present invention on non-woven fabrics fabric while photocuring, it is this
Non-woven fabrics fabric can make mouth mask or protective garment with NACF, and compared with traditional mouth mask and protective garment, it not only may be used
With physical absorption and the organic phosphorus compound excessively in air filtering, while it can also will adsorb superincumbent organic phosphorus compound degraded
For nontoxic material, protection effect is substantially increased.
Embodiment
With reference to embodiment, the present invention is specifically described.It should be appreciated that following embodiment is the excellent of the present invention
Embodiment is selected, to be better understood from the present invention, thus should not be regarded as limiting the scope of the present invention.
Embodiment 1
One kind photocuring enzyme method on non-woven fabrics fabric, comprises the following steps：
(1) outfit of water soluble photo-curable resin system precursor liquid：
Molecular weight is 600 polyethyleneglycol diacrylate：25.0%；
Ethoxyquin trimethylolpropane trimethacrylate：5.0%
Deionized water：68.0%；
Light trigger 651：1.0%；
Light trigger TPO：1.0%；
(2) the dissolved organic phosphorus hydrolase in above-mentioned precursor liquid, it is made into the blended liquid that organophosphor hydrolytic enzyme concentration is 10mg/ml；
(3) blended liquid configured is coated on non-woven fabrics, solidified under ultraviolet or visible-light curing light source；
(4) it is as follows in the enzyme activity method of testing of non-woven fabrics immobilised enzymes：
The immobilized non-woven fabrics for having enzyme of 1.0mg is added in 1ml Tris-HCl buffer solutions, then adds 5 μ l parathion-methyls
Methanol solution (10mg/ml), adds 1ml 10% trichloroacetic acid solution terminating reaction after reacting 10 minutes at 37 DEG C, then adds
Enter 1ml 10% Na2CO3Colour developing, absorbance of the solution in 400nm finally is surveyed with ultraviolet specrophotometer, according to paranitrophenol
Concentration-absorbance standard curve obtains the concentration of paranitrophenol.
After tested, the enzyme activity of enzyme immobilization is 10.0U/g on non-woven fabrics, and the enzyme activity rate of recovery is 80.0%, is preserved at room temperature
Enzyme activity still keeps 10.0U/g after 15 days.
Embodiment 2
One kind photocuring enzyme method on non-woven fabrics fabric, comprises the following steps：
(1) outfit of water soluble photo-curable resin system precursor liquid：
Molecular weight is 600 polyethyleneglycol diacrylate：25.0%；
Ethoxyquin trimethylol-propane trimethacrylate：5.0%
Deionized water：68.0%；
Light trigger 651：1.0%；
Light trigger TPO：1.0%；
(2) the dissolved organic phosphorus hydrolase in above-mentioned precursor liquid, it is made into the blended liquid that organophosphor hydrolytic enzyme concentration is 20mg/ml；
(3) blended liquid configured is coated on non-woven fabrics, solidifying to be made under ultraviolet or visible-light curing light source immobilized has
The non-woven fabrics of organophosphor hydrolytic enzyme, according to the method for testing of enzyme activity in embodiment 1, obtaining the enzyme activity of enzyme immobilization on non-woven fabrics is
20.8U/g, the enzyme activity rate of recovery are 83.2%, and enzyme activity still keeps 20.8U/g after preserving 15 days at room temperature.
Embodiment 3
One kind photocuring enzyme method on non-woven fabrics fabric, comprises the following steps：
(1) outfit of water soluble photo-curable resin system precursor liquid：
Molecular weight is 600 polyethyleneglycol diacrylate：25.0%；
Ethoxyquin trimethylolpropane trimethacrylate：5.0%
Deionized water：68.0%；
Light trigger 184：1.0%；
Light trigger TPO：1.0%
(2) the dissolved organic phosphorus hydrolase in above-mentioned precursor liquid, it is made into the blended liquid that organophosphor hydrolytic enzyme concentration is 40mg/ml；
(3) blended liquid configured is coated on non-woven fabrics, solidifying to be made under ultraviolet or visible-light curing light source immobilized has
The non-woven fabrics of organophosphor hydrolytic enzyme, according to the method for testing of enzyme activity in embodiment 1, obtaining the enzyme activity of enzyme immobilization on non-woven fabrics is
42.3U/g, the enzyme activity rate of recovery are 84.6%, and enzyme activity still keeps 42.3U/g after preserving 15 days at room temperature.
Embodiment 4
One kind photocuring enzyme method on non-woven fabrics fabric, comprises the following steps：
(1) outfit of water soluble photo-curable resin system precursor liquid：
Molecular weight is 600 polyethyleneglycol diacrylate：25.0%；
Ethoxyquin trimethylol-propane trimethacrylate：5.0%；
Deionized water：68.0%；
Light trigger 184：1.0%；
Light trigger TPO：1.0%
(2) the dissolved organic phosphorus hydrolase in above-mentioned precursor liquid, it is made into the blended liquid that organophosphor hydrolytic enzyme concentration is 60mg/ml；
(3) blended liquid configured is coated on non-woven fabrics, solidifying to be made under ultraviolet or visible-light curing light source immobilized has
The non-woven fabrics of organophosphor hydrolytic enzyme, according to the method for testing of enzyme activity in embodiment 1, obtaining the enzyme activity of enzyme immobilization on non-woven fabrics is
64.65U/g, the enzyme activity rate of recovery are 86.2%, and enzyme activity still keeps 64.65U/g after preserving 15 days at room temperature.
Claims (6)
- A kind of 1. method for fixing organophosphor hydrolytic enzyme on non-woven fabrics using photocuring technology, it is characterised in that methods described Comprise the following steps：（1）The blended liquid of water soluble photo-curable resin system and organophosphor hydrolytic enzyme is configured, light-cured resin system is by water solubility Acrylate prepolymer body and light trigger composition；（2）The blended liquid configured is coated on non-woven fabrics, solidified under ultraviolet or visible-light curing light source；Non-woven fabrics after will be immobilized is dried.
- 2. according to the method for claim 1, it is characterised in that the step（1）Middle water-soluble acrylic ester performed polymer is Polyethylene glycol acrylate, polyethyleneglycol diacrylate, polyethylene glycol methacrylate-styrene polymer, glycol dimethacrylates Ester, ethoxyquin epoxy acrylate, ethoxyquin epoxy methacrylates, ethoxyquin trimethylolpropane trimethacrylate, second Aoxidize one or more mixtures in trimethylol-propane trimethacrylate.
- 3. according to the method for claim 1, it is characterised in that the step（1）Middle light trigger is selected from：Initiator 1173rd, 651,184, TPO, ITX, 819,784, benzophenone, diaryl diazo salt diaryl group iodized salt, triaryl sulfonium salts, One or more in luxuriant molysite.
- 4. according to the method for claim 1, it is characterised in that the step（2）Described in photocuring light source be selected from high pressure Mercury lamp, Halogen lamp LED, dysprosium lamp and wavelength are 300-532nm LED light source.
- A kind of 5. non-woven fabrics for being loaded with organophosphor hydrolytic enzyme prepared by method using described in claim 1-4.
- 6. the non-woven fabrics as claimed in claim 5 for being loaded with organophosphor hydrolytic enzyme is preparing mouth mask, handkerchief, clothes, cap weaving Purposes in product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711040182.5A CN107828770A (en) | 2017-10-30 | 2017-10-30 | It is a kind of to solidify organophosphor hydrolytic enzyme method in non-woven fabrics glazing |
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| CN201711040182.5A CN107828770A (en) | 2017-10-30 | 2017-10-30 | It is a kind of to solidify organophosphor hydrolytic enzyme method in non-woven fabrics glazing |
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| CN107828770A true CN107828770A (en) | 2018-03-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321134A (en) * | 2020-02-27 | 2020-06-23 | 西南科技大学 | Immobilized multienzyme system and preparation method thereof |
Citations (5)
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|---|---|---|---|---|
| CN1317574A (en) * | 2001-01-15 | 2001-10-17 | 湖南大学 | Optical immobilizing method for piezoelectric sensing medium of enzyme and microbe |
| JP2006137738A (en) * | 2004-11-11 | 2006-06-01 | Yasuhara Chemical Co Ltd | Terpene-based (meth)acrylic acid ester and its curable composition |
| CN101140256A (en) * | 2007-10-19 | 2008-03-12 | 江南大学 | Preparation method of photofixation horse radish peroxidase electric pole and uses thereof |
| WO2016024566A1 (en) * | 2014-08-13 | 2016-02-18 | 三井化学株式会社 | Medical device, method for culturing cells, fluorine-containing cyclic olefin polymer, fluorine-containing cyclic olefin polymer composition, and cultured cells |
| CN106324054A (en) * | 2016-09-18 | 2017-01-11 | 江南大学 | Method based on photosensitive biomacromolecule-loaded enzyme to prepare biosensor |
-
2017
- 2017-10-30 CN CN201711040182.5A patent/CN107828770A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317574A (en) * | 2001-01-15 | 2001-10-17 | 湖南大学 | Optical immobilizing method for piezoelectric sensing medium of enzyme and microbe |
| JP2006137738A (en) * | 2004-11-11 | 2006-06-01 | Yasuhara Chemical Co Ltd | Terpene-based (meth)acrylic acid ester and its curable composition |
| CN101140256A (en) * | 2007-10-19 | 2008-03-12 | 江南大学 | Preparation method of photofixation horse radish peroxidase electric pole and uses thereof |
| WO2016024566A1 (en) * | 2014-08-13 | 2016-02-18 | 三井化学株式会社 | Medical device, method for culturing cells, fluorine-containing cyclic olefin polymer, fluorine-containing cyclic olefin polymer composition, and cultured cells |
| CN106324054A (en) * | 2016-09-18 | 2017-01-11 | 江南大学 | Method based on photosensitive biomacromolecule-loaded enzyme to prepare biosensor |
Non-Patent Citations (2)
| Title |
|---|
| FOTIOS M. ANDREOPOULOS等: "Photoimmobilization of Organophosphorus Hydrolase Within a PEG-Based Hydrogel", 《BIOTECHNOLOGY AND BIOENGINEERING》 * |
| ZÜMRÜT SEDEN AKDEMIR等: "Preparation and characterization of UV-curable polymeric support for covalent immobilization of xylanase enzyme", 《JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321134A (en) * | 2020-02-27 | 2020-06-23 | 西南科技大学 | Immobilized multienzyme system and preparation method thereof |
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Application publication date: 20180323 |