CN107828684A - Cooperateed with suitable for electronic microorganism and repair mix bacterium agent and its preparation and application petroleum hydrocarbon contaminated soil - Google Patents
Cooperateed with suitable for electronic microorganism and repair mix bacterium agent and its preparation and application petroleum hydrocarbon contaminated soil Download PDFInfo
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- CN107828684A CN107828684A CN201711116796.7A CN201711116796A CN107828684A CN 107828684 A CN107828684 A CN 107828684A CN 201711116796 A CN201711116796 A CN 201711116796A CN 107828684 A CN107828684 A CN 107828684A
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Abstract
Cooperateed with the invention belongs to electronic microorganism and repair petroleum hydrocarbon contaminated soil technical field, and in particular to be a kind of to repair mix bacterium agent and its preparation and application high salinity petroleum hydrocarbon contaminated soil suitable for the collaboration of electronic microorganism.Microbial inoculum ferments to mix respectively by celestial river Halomonas, bacillus licheniformis and bacillus cereus to be obtained；Present invention combination microbial inoculum is applied in electronic microorganism collaboration recovery technique, break the reparation bottleneck of the soil high salt state formed in repair process, function microbial inoculum resource rationally is utilized, effectively realizes the degraded of oil soil petrochina hydrocarbon, reduces soil petrochina hydrocarbon organic pollution toxicity.
Description
Technical field
The invention belongs to electronic-microorganism collaboration to repair petroleum hydrocarbon contaminated soil technical field, and in particular to one kind is applicable
Mix bacterium agent and its preparation and application in high salinity petroleum hydrocarbon contaminated soil are repaired in electronic-microorganism collaboration.
Background technology
The continuous development of petroleum economics, which is grown, causes the mining requirement amount of petroleum hydrocarbon to improve year by year, due to exploitation landing, fortune
The hydrocarbon quantity that the reasons such as defeated leakage enter soil is also continuously increased therewith, present environmental risks and ecological safety.
On the other hand, the improvement repair for oil-polluted soils is carried out rapidly and obtains more and more extensive concern.At present, with biology
And the petroleum hydrocarbon contaminated soil recovery technique based on chemical method is more favored.However, in view of petroleum hydrocarbon contaminant it is strong
Hydrophobic property, single microorganism remediation technology are difficult to the petroleum hydrocarbon degradation effect of efficient stable, on the other hand, passing through single place
The organic assembling of reason technology and the combined remediation technology that is formed are to realize that what oil-polluted soils recovery technique broke through has force direction.
Electric repairing technique is oil as a kind of organic polluted soil recovery technique to attract attention further in recent years
The new hope of soil contaminated with hydrocarbons reparation.However, also there is the adverse effect of remediation efficiency " hangover " in electric repairing technique.On the other hand,
Using electronic-microorganism collaboration recovery technique, based on microorganism remediation, microbial metabolism is realized by electro reclamation process
Reinforcing, will be it is quick start petroleum hydrocarbon contaminant degradation process, and maintain the effective means of fast degradation period.But at present,
The trend of soil property extramalization still be present in the collaboration recovery technique of electronic-microorganism, wherein, " the ion wall " of electrode tip is existing
As caused high salt is characterized in that the technology applies one of notable feature changed to soil property.High salt feature produces salt stress
Effect, cause many microorganisms to be dehydrated and lose breeding metabolic function, so mortality, lose petroleum hydrocarbon metabolic activity.It is right
This, will be one of feasible method of the adaptation petroleum hydrocarbon contaminated reparation of salt-affected soil from high salt-tolerant trait bacterial strain.
The content of the invention
Present invention aims at provide a kind of Mixed Microbes suitable for electronic-microorganism collaboration reparation petroleum hydrocarbon contaminated soil
Agent and its preparation and application.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of to repair mix bacterium agent high salinity petroleum hydrocarbon contaminated soil suitable for electronic-microorganism collaboration, microbial inoculum is by celestial being
River Halomonas, bacillus licheniformis and bacillus cereus, which ferment to mix respectively, to be obtained；Celestial river Halomonas is preserved in China
General Microbiological Culture collection, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preserving number CGMCC
NO.1.6848, at 2008 preservation day September 15 days, taxology name Halomonas xianhensis；Bacillus licheniformis and wax
Sample bacillus is preserved in China typical culture collection center, and address is wuchang, wuhan Luo Jia Shan, and preservation registration number divides
Wei not CCTCC NO：M 2011261 and M 2015705, preservation day are respectively on July 22nd, 2011 and on December 3rd, 2015, are divided
Class name is respectively Bacillus licheniformis and Bacillus cereus.
Three plants of bacterial strains are respectively provided with salt-tolerant trait, can be grown in the liquid environment that salinity is 3~5% (w/v), wherein, it is celestial
River Halomonas, bacillus licheniformis and bacillus cereus can be utilized respectively PAHs (luxuriant and rich with fragrance, anthracene or fluoranthene), alkane (C16 respectively
~C24) and aliphatic acid (palmitic acid, stearic acid) as sole carbon source carry out growth metabolism.
The microbial inoculum by celestial river Halomonas, bacillus licheniformis and bacillus cereus respectively ferment mixing after through carrier
Immobilization obtains.
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 2~3:5~6:1(v:v)
Ratio mixing and through fixing to obtain initial mixing microbial inoculum；
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 1:3~5:2~3 (v:v)
Ratio mix and obtain reinforcement mix bacterium agent through immobilization.
A kind of application that mix bacterium agent high salinity petroleum hydrocarbon contaminated soil is repaired suitable for electronic-microorganism collaboration, institute
State application of the microbial inoculum in the case where being acted on electric field synergistic to high salinity petroleum hydrocarbon contaminated soil reparation.
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 2~3:5~6:1(v:v)
Ratio mixing and through fixing to obtain initial mixing microbial inoculum；
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 1:3~5:2~3 (v:v)
Ratio mix and obtain reinforcement mix bacterium agent through immobilization.
The current field condition to apply 0.5~2.0v/cm DC electric field, using initial mixed by the beginning for carrying out electro reclamation
Microbial inoculum is closed, bacterial micro-organism quantity is up to 2~8 × 108CFU·g-1, when in contaminated soil alkane component degradation rate up to 40~50%
When, apply reinforcement mix bacterium agent, maintain bacteria total amount in soil to reach the 90~110% of initial content, repair whole process with electronic-micro-
The mode of biological synergetic reparation is carried out.
Celestial river Halomonas, bacillus licheniformis and bacillus cereus are respectively according to 2~3:5~6:1(v:And 1 v):3
~5:2~3 (v:V) ratio mixing, is respectively prepared initial mixing microbial inoculum and reinforcement mix bacterium agent, applied to electricity after immobilization
Repair process of the dynamic-microorganism collaboration recovery technique to oil-polluted soils；The acidified salinity of mixed bacteria liquid is up to 10~20%
(w/w) being fixed of carrier made of rice husk is handled, but is not limited to carry out bacterial strain immobilization using carbonization rice husk.
A kind of method for repairing high salinity petroleum hydrocarbon contaminated soil, to petroleum wastewater under the microbial inoculum and electric field synergistic are acted on
Contaminate soil remediation.
The beginning for being carried out electro reclamation using 0.5~2.0v/cm DC electric field to pending contaminated soil is used initially
Mix bacterium agent, make in soil bacterial micro-organism quantity up to 2~8 × 108CFU·g-1, when alkane component degradation rate in contaminated soil
During up to 40~50%, apply reinforcement mix bacterium agent, maintain soil in bacteria total amount reach initial soil in bacterium total content 90~
110%, repair whole process and carried out in a manner of electronic-microorganism collaboration is repaired.
Mix bacterium agent can grow in soil of the salt content up to 0.5%~2% (w/w), and have to petroleum hydrocarbon contaminant
There is metabolic degradation active.
Advantage for present invention includes：
The present invention, will be a variety of by screening the function degradation bacteria strains of different one-components in view of the complicated ingredient of petroleum hydrocarbon
Different petroleum hydrocarbon component metabolic function bacterial strains with high salt-tolerant trait carry out organic assembling and mixed bacteria liquid and immobilized bacterium are made
Agent, it can effectively realize and have complementary functions, degradation of contaminant toxicity, realize the collaboration Co metabolism effect between bacterial strain, pass through special formula
Proprietary microbial inoculum is prepared, to realize that electronic-microorganism of petroleum hydrocarbon contaminated soil cooperates with the lasting efficient process repaired, to solve to be somebody's turn to do
The defects of recovery technique application efficiency " hangover ", specifically：
1) celestial river Halomonas, bacillus licheniformis and the bacillus cereus in the present invention are respectively provided with resistance to high salt feature,
It is under the jurisdiction of Halophilic Bacterium, can be survived in the liquid environment that salinity is 3~5% (w/v), made mix bacterium agent equally has
There is resistance to high salt characteristic, so there is good adaptation energy for caused part during electro reclamation " ion accumulation " effect
Power, its growth activity are unaffected, while are more suitable for the reparation of oil-polluted soils in the salt-soda soil of field area
Using.
2) celestial river Halomonas, bacillus licheniformis and the bacillus cereus in the present invention have PAHs (luxuriant and rich with fragrance, anthracenes respectively
Or fluoranthene), the metabolic capability of alkane (C16~C24) and aliphatic acid (palmitic acid, stearic acid), and screened from petroleum hydrocarbon contaminated
In soil, thus while high salt soil environment is resistant to can targetedly to petroleum hydrocarbon in each specific components carry out metabolism drop
Solution, and with the immunological characteristic to non-specific degraded substrate, so as to ensure that it is sustainable under electric field-enhanced treatment conditions
Metabolic activity.
3) the initial mixing microbial inoculum in the present invention and reinforcement mix bacterium agent are metabolized bacterial strain in proportion by three kinds of difference in functionalitys
It is made, is used wherein repairing the beginning to be metabolized the mix bacterium agent of PAHs and alkane as chief component, for initial oil group
Divide and carry out degradation, when alkane degradation rate is up to 40~50%, now fatty acid material has and accumulated to a certain degree, feeds
The mix bacterium agent for improving fatty acid metabolism bacterial strain content ratio carries out the processing that continues, and can pointedly remove accumulated aliphatic acid
Class material, effectively across metabolism bottleneck, the toxic action of the aliphatic acid of improvement high accumulation amount, the group of promotion functions degradation flora
It is potent with function into stabilization.
Brief description of the drawings
Fig. 1 is the electric device ideograph used in the electrokinetic process participation group that the embodiment of the present invention 2 provides.
Electronic-microorganism cooperates with repair process A (EK-Bio- when Fig. 2 is the test process 35d of the offer of the embodiment of the present invention 2
A each position total salt content variation diagram of soil in).
Fig. 3 is soil pH and bacterial micro-organism quantity in each test process anode tap soil of the offer of the embodiment of the present invention 2
Variation diagram.
Fig. 4 is that each test process total petroleum hydrocarbon degradation rate that the embodiment of the present invention 2 provides changes over time figure.
Embodiment
Mix bacterium agent of the present invention is by celestial river Halomonas (Halomonas xianhensis) A-1 CGMCC
NO.1.6848, bacillus licheniformis (Bacillus licheniformis) CCTCC NO：M 2011261 and waxy gemma bar
Bacterium (Bacillus cereus) CCTCC NO：M 2015705 is made after being mixed by different proportion through immobilization, and three plants of bacterial strains are equal
With resistance to high salt characteristic, and the PAHs (luxuriant and rich with fragrance, anthracene or fluoranthene) being directed to respectively in petroleum hydrocarbon component, alkane (C16~C24) and fat
Sour (palmitic acid or stearic acid) has efficient metabolic capability.Celestial river Halomonas, bacillus licheniformis and bacillus cereus point
Not according to 2~3:5~6:1(V:V ratio mixing), is made initial mixing microbial inoculum after immobilization, puts on initial electronic-micro-
Biological synergetic is repaired in petroleum hydrocarbon contaminated soil, and bacterial micro-organism quantity is up to 4~8 × 108CFU·g-1.Work as alkane component degradation
When rate is up to 40~50%, feed by celestial river Halomonas, bacillus licheniformis and bacillus cereus respectively according to 1:3~5:2
~3 (V:V ratio mixing) and reinforcement mix bacterium agent made of immobilization, maintain bacteria total amount in soil to reach the 90 of initial content
~110%.Reinforcement microbial inoculum applies feeds organic carbon source and inorganic nutrients ion simultaneously.The combination microbial inoculum is applied to electronic-micro- life
In thing collaboration recovery technique, break the reparation bottleneck of the soil high salt state formed in repair process, rationally utilize function bacterium
Agent resource, the degraded of oil soil petrochina hydrocarbon is effectively realized, reduce soil petrochina hydrocarbon organic pollution toxicity.
Below in conjunction with the accompanying drawings and embodiment the present invention is described in further detail.
Embodiment 1 repairs the preparation of mix bacterium agent petroleum hydrocarbon contaminated soil suitable for electronic-microorganism collaboration
Bacterium source sample used in the present embodiment picks up from field area and included for a long time by petroleum hydrocarbon contaminated high content of salt soil, soil
Salt total ionic weight shakes the petroleum hydrocarbon contaminated salt-affected soils of suspension 5.0g up to 1.6% (w/w), using 30ml minimal mediums,
160rpm/min concussion and cultivate 20min, stand 3min, draw 1ml upper strata suspensions to contain respectively 100mg/L is luxuriant and rich with fragrance, 300mg/L just
Acclimating culture 15d in the inorganic salt liquid culture medium of three kinds of inorganic salts of hexadecane and 100mg/L palmitic acids, through solid inorganic
Salt culture medium line isolates and purifies identify after obtain with PAHs it is Sino-Philippines for the celestial river Halomonas of metabolizable carbon source, with hexadecane
Bacillus licheniformis for metabolizable carbon source and the bacillus cereus using palmitic acid as metabolizable carbon source.
The celestial river Halomonas is preserved in China General Microbiological DSMZ, and address is Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preserving number are CGMCC NO.1.6848, at 2008 preservation day September 15 days, taxology name
Halomonas xianhensis；Bacillus licheniformis and bacillus cereus are preserved in China typical culture collection
The heart, address are wuchang, wuhan Luo Jia Shan, and preservation registration number is respectively CCTCC NO：M 2011261 and M 2015705, preservation
Day be respectively on July 22nd, 2011 and on December 3rd, 2015, taxology name be respectively Bacillus licheniformis with
Bacillus cereus。
The composition of the inorganic salt liquid culture medium is：1.5g·L-1NaCl, 0.2gL-1MgSO4, 1.5gL- 1NH4NO3, 1.0gL-1K2HPO4, 0.5gL-1KH2PO4, 0.02gL-1CaCl2, 0.02gL-1FeSO4, 1L distilled water, pH
Value 7.0-7.3,121 DEG C of sterilizing 20min is standby.
The composition of the inorganic salts solid medium is：1.5g·L-1NaCl, 0.2gL-1MgSO4, 1.5gL- 1NH4NO3, 1.0gL-1K2HPO4, 0.5gL-1KH2PO4, 0.02gL-1CaCl2, 0.02gL-1FeSO4, 15gL-1Fine jade
Fat, 1L distilled water, addition pH value 7.0-7.3,121 DEG C of sterilizing 20min, solid culture primary surface coat 1ml concentration and are respectively
Diethyl ether solution, 10ul hexadecanes and 1ml concentration luxuriant and rich with fragrance 1mg/ml is 1mg/ml palmitic acid hexane solutions, treats that organic solvent is waved
It is standby after hair.
Three plants of bacterial strains difference single colony inoculation of picking of preservation is contained to 3% (w/v) NaCl beef extract-peptone in 5ml
In fluid nutrient medium, 37 DEG C of concussion and cultivate 16h, seed liquor is obtained.300ul strain culturing seed liquors are drawn respectively to be further inoculated with
Expand to the beef extract-peptone containing 3% (w/v) NaCl in culture medium, 37 DEG C of concussion and cultivates 48h, 6000rpmmin-1Centrifugation
10min is to collect thalline.Collected thalline is respectively adopted minimal medium and cleaned, and uses minimal medium system
It is standby into thalline suspension.By celestial river Halomonas, bacillus licheniformis and bacillus cereus bacteria suspension respectively according to 2.5:5:1
(v:And 1 v):4:3(v:V) ratio is mixed and made into initial mixing bacterium solution and reinforcement mixed bacteria liquid.
The composition of the inorganic salt liquid culture medium is：3.0g/L beef extracts, 10g/L peptones, 5.0g/L NaCl, steam
Distilled water 1L, pH 7.0.
Carbonization treatment is carried out to complete rice husk using 50% (w/w) sulfuric acid, when rice husk mineralization rate is up to 15%, using ammoniacal liquor
After neutralisation of sulphuric acid system to neutrality, clear water is rinsed and air-dried, and is added according to 20% (w/v, g/ml) ratio into mixed bacteria liquid,
Being fixed is handled under the conditions of 4 DEG C, and initial mixing microbial inoculum and reinforcement mix bacterium agent is made.Wherein, initial mixing microbial inoculum has
Have has stronger Fatty acid degradation with alkane in petroleum hydrocarbon component and the degradation characteristic based on aromatic hydrocarbon metabolism, reinforcement mix bacterium agent
Characteristic.
Application of the mix bacterium agent of embodiment 2 in petroleum hydrocarbon contaminated soil is repaired in electronic-microorganism collaboration
Contaminated soil used in experiment picks up from field area petroleum hydrocarbon contaminated soil, and soil petrochina hydrocarbon content is up to 4.6% (w/
W), pending soil is after removing visible weeds impurity of naked eyes etc., natural air drying, and it is standby to cross 2mm sieves.Soil initial properties bag
Include, pH 7.2, the total ion salt content 1.2% (w/w) of soil, soil water content is adjusted to 23% (w/w) pending use afterwards.
Experiment is set as shown in table 1, sets six groups of processing altogether, is designated as compareing (CK), electrokinetic process A (EK-A), electricity respectively
Dynamic processing B (EK-B), microbiological treatment (Bio), electronic-microorganism collaboration processing A (EK-Bio-A) and electronic-microorganism collaboration
Handle B (EK-Bio-B).
Repair process group (Bio, EK-Bio-A, EK-Bio-B) the processing beginning containing microorganism applies as in embodiment 1
Prepared initial mixing microbial inoculum, after application in soil bacterial micro-organism quantity up to 5.2 × 108CFU·g-1.Petroleum hydrocarbon contaminated soil
Earth electro reclamation device is long 28cm × wide 15cm × high 14cm box body (Fig. 1), and it is dirty to load 1600g petroleum hydrocarbons in each box body
Contaminate soil；Electro reclamation electrode used therein uses stainless steel stick electrode, electrode diameter 1.2cm, a height of 18cm, between two lateral electrodes
Away from for 24cm, apply 24V DC voltages.
The basic condition that 1 each test process of table is set and test parameters table.
Experiment is a sampling period per 7d, monitors petroleum hydrocarbon and petroleum hydrocarbon Group Component content after sample collection, works as oil
When alkane total degradation rate is up to 42% in hydrocarbon system's component, apply reinforcement mix bacterium agent, make in soil bacterial micro-organism total amount up to 6.1 ×
108CFU·g-1, continue repair process, electrokinetic process group B (EK-B) and electronic-microbiological treatment group (EK-Bio-A and
EK-Bio-B regular tracking and monitoring soil pH value Parameters variation in), when anode tap pH value is less than 6 or cathode terminal pH value is more than 8
When feed 1mol/L HCl or 1mol/L NaOH and carry out pH value to regulate and control in the range of 6~8, total repairing efficiency reaches 63d.
Result of the test shows, with the lasting progress of electrokinetic process, extreme soda acid occurs in soil pH, influences the micro- life of bacterium
Thing quantity (Fig. 3)；However, by adjusting level of the Effective Regulation soil pH 6~7, but electrode both sides total salt content in soil
Significantly improved in the electrokinetic process later stage, the soil salinization is obvious (Fig. 2), causes to have obtained the electronic place of effective regulation in soil pH
Indigenous microorganism quantity still significantly reduces in reason B (EK-B).After initial mixing microbial inoculum B is applied, micro organism quantity is had
Effect maintains (Fig. 3), but handles the later stage, and the accumulation of organic acid limits the activity of microorganism herein, when in electronic-microorganism collaboration
Handle after applying reinforcement mix bacterium agent in B, micro organism quantity is fed, and metabolic activity is effectively continued.Total oil
Alkane degradation rate measure shows that the degraded that the administration of initial mixing microbial inoculum has substantially speeded up younger oil hydrocarbon removes, and reinforcement microbial inoculum
Addition then further optimize the high-efficiency continuous degradation process of petroleum hydrocarbon, after handling 63d, petroleum hydrocarbon total degradation rate reaches
40.1% (Fig. 4), the degradation rate for not applying the treatment group petroleum hydrocarbon of reinforcement mix bacterium agent improve 8%.It is indicated above that Mixed Microbes
Agent is suitable for the application in electronic-microorganism collaboration recovery technique processing, reaches and overcomes the unfavorable of electrode tip " ion accumulation "
The purpose of condition；And it is directed to the effect of having complementary functions of alkane, PAHs and fatty acid metabolism so that initial mixing microbial inoculum and reinforcement
The combination application mode of mix bacterium agent is more beneficial for playing the repair function of function microbial inoculum.
Claims (8)
1. a kind of repair mix bacterium agent high salinity petroleum hydrocarbon contaminated soil suitable for electronic-microorganism collaboration, it is characterised in that：
Microbial inoculum ferments to mix respectively by celestial river Halomonas, bacillus licheniformis and bacillus cereus to be obtained；Celestial river Halomonas is protected
China General Microbiological DSMZ is hidden in, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and preserving number is
CGMCC NO.1.6848, at 2008 preservation day September 15 days, taxology name Halomonas xianhensis；Lichens gemma bar
Bacterium and bacillus cereus are preserved in China typical culture collection center, and address is wuchang, wuhan Luo Jia Shan, and preservation is stepped on
Mark is respectively CCTCC NO：M 2011261 and M 2015705, preservation day are respectively on July 22nd, 2011 and in December, 2015
3, taxology name was respectively Bacillus licheniformis and Bacillus cereus.
2. repair Mixed Microbes high salinity petroleum hydrocarbon contaminated soil suitable for electronic-microorganism collaboration as described in claim 1
Agent, it is characterised in that：Microbial inoculum by celestial river Halomonas, bacillus licheniformis and bacillus cereus respectively ferment mixing after through carry
Body immobilization obtains.
3. repairing high salinity petroleum hydrocarbon contaminated soil and mixing suitable for electronic-microorganism collaboration as described in claim 1 or 2
Microbial inoculum, it is characterised in that：
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 2~3:5~6:1(v:V) ratio
Example mixes and through fixing to obtain initial mixing microbial inoculum；
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 1:3~5:2~3 (v:V) ratio
Example mixes and obtains reinforcement mix bacterium agent through immobilization.
A kind of 4. repairing high salinity petroleum hydrocarbon contaminated soil and mixing suitable for electronic-microorganism collaboration described in claim 1
The application of microbial inoculum, it is characterised in that：The microbial inoculum with electric field synergistic in the case where acting on to high salinity petroleum hydrocarbon contaminated soil reparation
Using.
5. repair Mixed Microbes high salinity petroleum hydrocarbon contaminated soil suitable for electronic-microorganism collaboration as described in claim 4
The application of agent, it is characterised in that：Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension according to 2~
3:5~6:1(v:V) ratio mixes and through fixing to obtain initial mixing microbial inoculum；
Celestial river Halomonas, bacillus licheniformis and the bacillus cereus bacteria suspension are according to 1:3~5:2~3 (v:V) ratio
Example mixes and obtains reinforcement mix bacterium agent through immobilization.
6. repairing high salinity petroleum hydrocarbon contaminated soil and mixing suitable for electronic-microorganism collaboration as described in claim 4 or 5
The application of microbial inoculum, it is characterised in that：The current field condition carries out electro reclamation to apply 0.5~2.0v/cm DC electric field
Beginning uses initial mixing microbial inoculum, and bacterial micro-organism quantity is up to 2~8 × 108CFU·g-1, when alkane component drops in contaminated soil
When solution rate is up to 40~50%, apply reinforcement mix bacterium agent, maintain bacteria total amount in soil to reach the 90~110% of initial content, repair
Multiple whole process is carried out in a manner of electronic-microorganism collaboration is repaired.
A kind of 7. method for repairing high salinity petroleum hydrocarbon contaminated soil, it is characterised in that：By described in claim 1-3 any one
To oil-polluted soils reparation under microbial inoculum and electric field synergistic effect.
8. the method for the remedying oil-polluted soils as described in claim 7, it is characterised in that：Pending contaminated soil is used
The beginning that 0.5~2.0v/cm DC electric field carries out electro reclamation uses initial mixing microbial inoculum, makes bacterial micro-organism number in soil
Measure up to 2~8 × 108CFU·g-1, when alkane component degradation rate is up to 40~50% in contaminated soil, apply reinforcement mix bacterium agent,
Maintain bacteria total amount in soil to reach 90~110% of bacterium total content in initial soil, repair whole with electronic-microorganism collaboration
The mode of reparation is carried out.
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Cited By (6)
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| CN111440741A (en) * | 2020-04-03 | 2020-07-24 | 中国科学院沈阳应用生态研究所 | Functional bacterium suitable for restoring petroleum-polluted soil in direct-current electric field anode region and application |
| CN112692051A (en) * | 2021-01-25 | 2021-04-23 | 明志科技大学 | Method and equipment for removing pollutants by using beneficial microorganism mixed bacterial liquid |
| CN113388542A (en) * | 2021-06-09 | 2021-09-14 | 中国科学院沈阳应用生态研究所 | Polyhalogenated aromatic hydrocarbon degrading bacteria and application thereof |
| CN114231439A (en) * | 2021-11-22 | 2022-03-25 | 中化珠海石化储运有限公司 | Microbial preparation for degrading oil in high-salinity water |
| CN114480189A (en) * | 2022-01-23 | 2022-05-13 | 北方民族大学 | Microbial compound inoculant and preparation method and application thereof |
| CN116286461A (en) * | 2022-12-08 | 2023-06-23 | 山东碧蓝生物科技有限公司 | Bacillus salicifolius with soil improvement effect, screening method and application thereof |
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| CN111440741A (en) * | 2020-04-03 | 2020-07-24 | 中国科学院沈阳应用生态研究所 | Functional bacterium suitable for restoring petroleum-polluted soil in direct-current electric field anode region and application |
| CN111440741B (en) * | 2020-04-03 | 2022-05-31 | 中国科学院沈阳应用生态研究所 | Functional bacterium suitable for restoring petroleum-polluted soil in direct-current electric field anode region and application |
| CN112692051A (en) * | 2021-01-25 | 2021-04-23 | 明志科技大学 | Method and equipment for removing pollutants by using beneficial microorganism mixed bacterial liquid |
| CN113388542A (en) * | 2021-06-09 | 2021-09-14 | 中国科学院沈阳应用生态研究所 | Polyhalogenated aromatic hydrocarbon degrading bacteria and application thereof |
| CN113388542B (en) * | 2021-06-09 | 2022-05-31 | 中国科学院沈阳应用生态研究所 | Polyhalogenated aromatic hydrocarbon degrading bacteria and application thereof |
| CN114231439A (en) * | 2021-11-22 | 2022-03-25 | 中化珠海石化储运有限公司 | Microbial preparation for degrading oil in high-salinity water |
| CN114231439B (en) * | 2021-11-22 | 2023-11-14 | 中化珠海石化储运有限公司 | Microbial preparation for degrading oil in high-salinity water |
| CN114480189A (en) * | 2022-01-23 | 2022-05-13 | 北方民族大学 | Microbial compound inoculant and preparation method and application thereof |
| CN114480189B (en) * | 2022-01-23 | 2023-09-08 | 北方民族大学 | Microbial composite microbial agent and preparation method and application thereof |
| CN116286461A (en) * | 2022-12-08 | 2023-06-23 | 山东碧蓝生物科技有限公司 | Bacillus salicifolius with soil improvement effect, screening method and application thereof |
| CN116286461B (en) * | 2022-12-08 | 2023-09-08 | 山东碧蓝生物科技有限公司 | Bacillus salicinus with soil improvement effect, screening method and application thereof |
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