CN107828679A - The rose-red Rhodococcus strain XHRR1 of ammonia and its application in a kind of purifying aquatic water - Google Patents
The rose-red Rhodococcus strain XHRR1 of ammonia and its application in a kind of purifying aquatic water Download PDFInfo
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Abstract
The invention discloses a kind of rose-red Rhodococcus strain XHRR1 of ammonia in purifying aquatic water, strain X HRR1 preservation are entitled：Rose rhodochrous XHRR1, Classification And Nomenclature are：Rhodococcus rhodochrous XHRR1, depositary institution are：China typical culture collection center, preservation address are：Wuhan, China, preservation date are：On August 3rd, 2017, deposit number are：CCTCC NO：M 2017437.Strain X HRR1 has stronger detergent power to the ammonia in breeding water body, and environmental suitability is good, and cultured prawn is had no adverse effects.Also disclose the application of above-mentioned rose-red Rhodococcus strain XHRR1 ammonia in purifying aquatic water.
Description
Technical field
The invention belongs to rose rhodochrous technical field, and in particular to the rose of ammonia is red in a kind of purifying aquatic water
Meningitidis strains XHRR1 and its application.
Background technology
In the intensive aquaculture pattern of high density, the efficient control of water body environment is the key of whole production process
One of sport technique segment.Ammonia nitrogen has serious toxic action to aquatic livestock, easily a large amount of in high-density breeding water body to accumulate (Zhou Ping
2013) content for, therefore in Effective Regulation water body being harmful to nitrogen is (Liu Xingguo 2011) of crucial importance.Domestic and international common place at present
Reason method mainly has three kinds of methods such as physics, chemistry and biology.Physical commonly uses thing using the adsorption capacity purification ammonia nitrogen of solid
Material mainly has zeolite powder, dolomite dust, coral sand etc., but because its adsorption cleaning is (Rao Li 2016) limited in one's ability, is given birth in cultivation
It is more during production to alleviate stress of the harmful nitrogen such as ammonia nitrogen to aquaculture organism by way of row changes water.Chemical method passes through application
The harmful substance in the Strong oxdiative effect purifying water body of oxidant is learned, but potential pollution source can not be thoroughly eradicated in production application
Accumulation problem, generally require to be used for multiple times and can be only achieved good result, the cost that it is used is of a relatively high, if misoperation is also
Easily cause the potential security risk (Lin Hong 2006) of water ecological setting.Bioanalysis utilizes specific microorganism sorption enhanced water
The harmful substances such as ammonia nitrogen, nitrite in body, in contrast with environment-friendly, it is not likely to produce secondary pollution, sustainability
The advantages that strong (Wang Zhanwei etc. 2013).Li Changling etc. (2008) introduces various concentrations in the water body for cultivating Sarotherodon sp seedling
Nitrifier, as a result show, when the concentration of initial nitrifier is in 100CFU/L, ammonia-nitrogen content reduces by 25.05% compared with control group, fish
Shoot survival percent improves 7.58% compared with control group, increased weight 46.15%；Beam supports the army etc. (2005) using Australian silverbelly as research pair
As a result showing, by launching nitrifier in advance and periodically additional nitration bacterium can delay into water body with the time interval of 7-10 days
The accumulation of ammoniacal nitrogen and nitrite is solved, improves breeding environment.It can be seen that scientific application nitrifier is favourable in aquaculture process
In the accumulation for solving the problems, such as harmful nitrogen, play and purify water, promote the good result of aquaculture organism healthy growth.Report
Autotrophic nitrification bacterium mainly includes Nitrobacter (Nitrobacter) (Zheng Jin to wait 2003), Nitrospina
(Nitrospina) (Li Junwen etc. 2004), Nitrococcus (Nitrococcus) (Bartosch, et al 1999), nitrification
Spiral Pseudomonas (Nitrospira) (Koops, et al.1990), Nitromonas (Nitrosomonas) (Grunditz
2001), Nitrosospira category (Nitrosospira) (Shaw, et al.2006), Nitrosolobus
(Nitrosolobus) (Webster 1996), Nitrosococcus (Nitrosococcus) (Klotz, et al.2006), Asia
Nitrify vibrio (Nitrosovibrio) (Ida, et al.2004).Wherein, autotrophic ammonia oxidizing bacterium belongs to deformation Gammaproteobacteria
(Proteobacteria) β subclasses and γ subclasses；Nitrification bacteria includes (Spiller, the et such as bacterium, fungi, actinomyces
Al.1976), such as：Arthrobacter globiformis (Arthrobacter globiformis) (Wang little Juan etc. 2009), Pseudomonas aeruginosa
(Pseudomonas aeruginosa) (Li Qiang etc. 2015), aspergillus parasiticus (Aspergillus parasiticus) (He Xia etc.
2006).However, because nitrifier is difficult to isolate and purify, domestic and international most researchers are all to be enriched with training using activated sludge
Nitrifier is supported, training method (Yang Ning 2003 is seldom expanded using pure bacterium；Wang Juan 2006).Golden will has just waited (1998) dirty using activity
Mud enrichment nitrifier shows when temperature is 30 DEG C, pH 6.5-8.0, dissolved oxygen amount are higher than 2.0mg/L, by the enrichment of 1-13 weeks
Culture, it is 12.5-20 times that bacteria concentration is nitrified in not enriched sludge.Shan and Obbard (2001) uses the nitrifier of immobilization
Processing lobster breeding wastewater obtains preferable effect.Liu Lingli (2012) is with immobilization way research fresh water type nitrifier, sea
Water type nitrifier, as a result fresh water type nitrifier is 0.12mg/gh to the clearance of ammonia nitrogen, clearance of the sea-water type bacterium to ammonia nitrogen
For 0.13mg/gh.
Rhodococcus sp can survive in environment is polluted under field conditions (factors), can be used as the inoculation amboceptor of bio-decontaminated, Rhodococcus sp
The bacterium of category has conversion and degradation to many compounds, it can be used as into biosurfactant and biological flocculant.It is red
The cell surface active matter such as mycolic acidses of coccus can reduce the surface tension of interface, make hydrophilic compounds be more easy to enter thalline
Cell, add the intrusion face of thalline；In addition, Rhodococcus sp, which produces, can also produce the glycolipid flocculation formed by polypeptide, lipid aggregation
Material, flocculation is produced to many suspensions, contribute to the removing (the careless root 2003 of China) of suspension in waste water or waste processing.
There is large-scale genome and linear plasmid in Rhodococcus sp thalline, this is beneficial to accommodate substantial amounts of oxidizing ferment and other enzyme system materials,
Rhodococcus sp is enabled to make full use of the energy and carbon source from organic compound, and can also output carotenoid (Qiu Zibo
2016)。
Although immobilized microorganism technology receive much concern also carried out it is many benefit our pursuits and attempt, at present mostly also
Only rest on small-scale, the application experiment stage of small range, the efficient application effect of also not up to extensive industrialization.To be effective
Promote safe and efficient application of the correlation technique in aquaculture industry, it is necessary to based on breeding water body environment, therefrom screen
The indigenous strain with harmful nitrogen element purification function is obtained, and analyzes the environmental suitability of bacterial strain, ecological functions efficiency, using peace
The features such as full property, and then research and develop the microbial inoculum product and application technology of suitable aquaculture production practical application.Rather than simply apply mechanically
Correlative detail in sewage treatment project technology, ignore aquaculture organism with production to safe efficient, sustainable development specific reality
Border demand.Have no at present on the phase of rose rhodochrous (Rhodococcus rhodochrous) purifying aquatic water ammonia
Close the research report of bacterial strain.
The content of the invention
It is an object of the invention to provide a kind of rose-red Rhodococcus strain XHRR1 of ammonia in purifying aquatic water, the bacterium
Strain XHRR1 has stronger detergent power to the ammonia in breeding water body, and environmental suitability is good, to cultured prawn without bad shadow
Ring.
The present invention also aims to provide above-mentioned rose-red Rhodococcus strain XHRR1 in purifying aquatic water ammonia should
With.
First purpose of the present invention is achieved by the following technical solution：The rose of ammonia in a kind of purifying aquatic water
Rhodochrous strain X HRR1, strain X HRR1 preservation are entitled：Rose rhodochrous XHRR1, Classification And Nomenclature are：
Rhodococcus rhodochrous XHRR1, depositary institution are：China typical culture collection center, preservation address are：In
State Wuhan, preservation date are：On August 3rd, 2017, deposit number are：CCTCC NO：M 2017437.
The screening of rose rhodochrous XHRR1 in the present invention separates qualification process：Choose the intensive high density of prawn
The later stage breeding water body (cultivation 50~75 days) of cultivating pool, water sample is filtered with 0.22 μm of mixed ester membranes, then will
The filter membrane that have accumulated microbiological specimens is placed in closed concussion and cultivate 3~5 days, temperature 25~30 in photosynthetic bacteria liquid culture medium
DEG C, 2500~4000lx of intensity of illumination；Cultured bacterium solution is subjected to line culture on photosynthetic bacteria solid plate culture medium,
Culture 3~5 days, the single bacterium colony of different shape is selected, isolate and purify and obtain the good bacterial strain of growth performance.Then inoculation extremely
Photosynthetic bacteria liquid culture medium, 28~30 DEG C, intensity of illumination 2500~4000lx, 150~200rpm carry out shaking table and expand culture 3
~5 days.Different bacterium solutions is respectively added to have adjusted NH4(with NH in the sterilizing cultivation water of Cl concentration4Ammonia in Cl regulation water bodys
Concentration to 10~30mg/L), 28~30 DEG C, intensity of illumination 2500~4000lx, 150~200rpm carry out shaking table and expand culture
3~5 days.Choose the bacterial strain progress strain idenfication that can effectively reduce water body ammonia density and conservation is standby.
Separation obtains bacterial strain and carries out pure culture from the cultivation middle and later periods water body in intensive culture pond, is put by bacterium solution
Enter with NH4Cl allotments contain high strength ammonia (with NH4The concentration of ammonia is to 10~30mg/L in Cl regulation water bodys) breeding water body in,
Analyze bacterial strain influences on the clean-up effect of ammonia in water body, obtains the bacterial strain of energy high-efficient purification breeding water body ammonia, and determine the bacterial strain
Condition of culture.
The rose rhodochrous XHRR1 15~35 DEG C of temperature, salinity 5~45, pH6~9 can normal growth, optimal bar
Part is 15~35 DEG C of temperature, salinity 25~45, pH7.0~8.5.At optimum conditions with growing 2 in breeding water body nutrient environment
It or so can reach peak value, and 10 are maintained during 2~5 days8CFU/mL quantity levels.The bacterial strain suitably should in most of pond
With.
Second object of the present invention is achieved through the following technical solutions：Above-mentioned rose-red Rhodococcus strain
The application of XHRR1 ammonia in purifying aquatic water.
The rose rhodochrous XHRR1 grows under breeding water body nutrient environment can reach peak value for 2 days or so, 2~5 day phase
Between maintain 108CFU/mL quantity levels.The bacterial strain is suitably applied in most of pond.The bacterial strain is placed in containing high ammonia nitrogen
In breeding water body, when water salinity is 5~45,30 DEG C of temperature, the clearance for adding ammonia in bacterium group water body can reach 79% for 3 days~
99%；When salinity reaches 25~45, the clearance of water body ammonia is more than 90%.When water temperature is 15~35 DEG C, during salinity 25, add bacterium
The clearance of ammonia can reach more than 90% in 3 days in group water body；When water temperature is less than 15 DEG C or higher than 40 DEG C, rose rhodochrous
XHRR1's is significantly affected except ammonia effect.
Moreover, with 10 in experiment training water body7When CFU/mL concentration applies the bacterium, test 7 days of Environment of Litopenaeus vannamei Low into
Motility rate can reach more than 90%, show it to aquaculture organism without obvious harmful effect.
In the present invention there are greatest differences in cultivating pool environment with the water body environment in background technology, it is necessary to cultivation water
Based on body environment, therefrom screening obtains the indigenous strain with nitrification function, and the environmental suitability of analysis and evaluation bacterial strain, life
The features such as state functional efficiency, application security and then research and develop the microbial inoculum product of suitable aquaculture production practical application and using skill
Art.Rather than simply apply mechanically correlative detail in sewage treatment project technology, ignore aquaculture organism and production to it is safe efficient, can
The specific actual demand of sustainable development.Therefore, the present invention by the rose rhodochrous XHRR1 that separates, screen to breeding water body
In ammonia there is obvious removal to act on, Environment of Litopenaeus vannamei Low does not have obvious harmful effect.Supported for further research and development suitable for aquatic products
Grow the nitrification bacteria agent of practical application request and supporting technology provides theory and technology support.
Compared with prior art, the invention has the advantages that：
(1) the rose rhodochrous XHRR1 in the present invention is notable and right to the removal effect of ammonia in intensive culture water body
Aquaculture organism has no adverse effects；
(2) water bodys of the rose rhodochrous XHRR1 in the present invention screened from intensive culture mid-term, has good ring
Border adaptability, suitable most cultivating pool water body application；
(3) the rose rhodochrous XHRR1 in the present invention can reach good applied to the purification of water quality regulation and control of intensive culture
Good application effect, be advantageous to be greatly decreased the water body during breeding production and change, can also need not the expensive water of configuration rates
Matter cleaning equipment, it can be further research and development or implement cultivation water environment orientation control technique, promote the aquatic products for realizing ecological, environmental protective
The development of efficient health aquaculture industry provides technological reserve with supporting.
Brief description of the drawings
Fig. 1 is the growth curve of bacterial strain rose rhodochrous XHRR1 in embodiment 3；
Fig. 2 is the change curve of bacterial strain rose rhodochrous XHRR1 purifying water body ammonia under different salinity in embodiment 3；
Fig. 3 is the change curve of purifying water body ammonia under bacterial strain rose rhodochrous XHRR1 different temperatures in embodiment 3；
Fig. 4 is influences of the bacterial strain rose rhodochrous XHRR1 to Environment of Litopenaeus vannamei Low in embodiment 4.
Embodiment
The present invention will be further described with reference to the accompanying drawings and examples, but the invention is not limited in any way.
The rose rhodochrous XHRR1 of the purifying aquatic water ammonia of embodiment 1 screening and culture
1st, material prepares
1.1st, bacterium source
In intensive prawn culturing pond, collection cultivates the water sample of 50~75 days, is divided with photosynthetic bacteria culture medium flat board
From culture.
1.2nd, culture medium
(1) photosynthetic bacteria liquid culture medium：CH3COONa：1g, yeast extract：1g、MgSO4·7H2O：0.4g、NaCl：
0.1g、CaCl2·2H2O：0.05g、NaHCO3：0.3g、KH2PO4：1g, trace element solution 1mL, above medicine are dissolved in steaming respectively
In distilled water, and 1000mL is dissolved to, pH7.0.
Trace element solution：EDTA：2.5g、ZnSO4·7H2O：10.95g、MnSO4·H2O：1.54g、CuS04·5H2O：
0.39g、CoCl2·6H2O：0.2g、FeSO4·7H2O：7g, above medicine is dissolved in distilled water respectively, and is dissolved to 1000m1,
pH7.0。
(2) photosynthetic bacteria solid plate culture medium：Agar powder 20g/ is added on the basis of photosynthetic bacteria liquid culture medium
L, it is prepared into solid plate culture medium.
2nd, the screening and culturing of bacterial strain
The later stage breeding water body (cultivation 50~75 days) in the intensive high-density breeding pond of prawn is chosen, with 0.22 μm mix
Condensating fiber element ester membrane filtration water sample, then the filter membrane that have accumulated microbiological specimens is placed in closed in photosynthetic bacteria liquid culture medium
Concussion and cultivate 3~5 days, 25~30 DEG C of temperature, 2500~4000lx of intensity of illumination；By cultured bacterium solution in photosynthetic bacteria solid
Line culture is carried out on plating medium, is cultivated 3~5 days, is selected the single bacterium colony of different shape, isolate and purify acquisition growth performance
Good bacterial strain.Then inoculation to photosynthetic bacteria liquid culture medium, 28~30 DEG C, intensity of illumination 2500~4000lx, 150
~200rpm carries out shaking table and expands culture 3~5 days.Different bacterium solutions is respectively added to have adjusted NH4The sterilizing of Cl concentration is supported
Grow in water (with NH4The concentration of ammonia is to 10~30mg/L in Cl regulation water bodys), 28~30 DEG C, 2500~4000lx of intensity of illumination,
150~200rpm carries out shaking table and expands culture 3~5 days.The bacterial strain progress strain idenfication of water body ammonia density can effectively be reduced by choosing.
Separation obtains bacterial strain and carries out pure culture from the cultivation middle and later periods water body in intensive culture pond, is put by bacterium solution
Enter with NH4In the breeding water body containing high strength ammonia of Cl allotments, analysis bacterial strain influences on the clean-up effect of ammonia in water body, obtains energy
The bacterial strain of high-efficient purification breeding water body ammonia, and determine the condition of culture of the bacterial strain.
The rose rhodochrous XHRR1 of the purifying aquatic water ammonia of embodiment 2 identification
The present invention has carried out the identification of 16S rDNA molecules to the rose rhodochrous XHRR1 of purifying aquatic water ammonia, from
Molecular level, and combination Gern morphology feature and Analysis of The Physiological And Biochemical Properties determine the kind of bacterial strain.16S rDNA sequences point
Analysis is essentially according to following steps：
1st, bacterial genomes DNA extraction：
(1) with sterile toothpick choose single bacterium colony be inoculated in expand culture medium in cultivate；
(2) inoculum 1.5mL, 10000rpm (11,500g) is taken to centrifuge 1 minute, exhaust supernatant as far as possible；
(3) 200 μ L buffer solution GA are added into bacterial sediment, vibrates to thalline and thoroughly suspends, it is final concentration of to add 180 μ L
20mg/mL lysozyme, 37 DEG C are handled more than 30 minutes；
(4) 20 μ L Proteinase K Solutions are added into pipe, are mixed；
(5) 220 μ L buffer solution GB are added, are vibrated 15 seconds, 70 DEG C are placed 10 minutes, and solution becomes limpid, and brief centrifugation is to go
Except the globule of cap wall；
(6) plus 220 μ L absolute ethyl alcohols, fully vibration mixes 15 seconds, and brief centrifugation is to remove the globule of cap wall；
(7) previous step resulting solution and flocculent deposit all add in an adsorption column CB3 to (adsorption column is put into collecting pipe
In), 12000rpm (13,400 × g) is centrifuged 30 seconds, outwells waste liquid, adsorption column CB3 is put into collecting pipe；
(8) 500 μ L buffer solutions GD, 12000rpm (13,400g) are added into adsorption column CB3 to centrifuge 30 seconds, outwell waste liquid,
Adsorption column CB3 is put into collecting pipe；
(9) 700 μ L rinsing liquids PW, 12000rpm (13,400g) are added into adsorption column CB3 to centrifuge 30 seconds, outwell waste liquid,
Adsorption column CB3 is put into collecting pipe；
(10) 500 μ L rinsing liquids PW, 12000rpm (13,400g) are added into adsorption column CB3 to centrifuge 30 seconds, are outwelled useless
Liquid, adsorption column CB3 is put into collecting pipe；
(11) adsorption column CB3 is put back in collecting pipe, 12000rpm (13,400g) is centrifuged 2 minutes, outwells waste liquid.It will inhale
Attached column CB3 is placed in room temperature and placed several minutes, thoroughly to dry rinsing liquid remaining in sorbing material；
(12) adsorption column CB3 is transferred in a clean centrifuge tube, the hanging dropwise addition 50 in middle part to adsorbed film~
200 μ L elution buffer TE, room temperature place 2~5 minutes, 12000rpm (13,400g) centrifuge 2 minutes, by solution be collected into from
In heart pipe；
(13) DNA concentration and purity detecting
Reclaim obtained DNA fragmentation agarose gel electrophoresis and UV spectrophotometer measuring concentration and purity.
2nd, the PCR amplifications of 16S rDNA genes
Bacterial universal primers are closed by company of Invitrogen (Shanghai) Trading Co., Ltd. used by 16S rDNA amplification
Into forward primer (8f) is：5’-AGAGTTTGATCCTGGCTCAG-3’；Reverse primer (1492r) is：5’-
GGTTACCTTGTTACGACTT-3’.50 μ L PCR reaction systems include：Sterilize the μ L of distilled water 37, primer each 1 μ L, dNTPs
(2.5mmol/L), 1 μ L, 10 × PCR buffer of Tap enzymes 5 μ L, the μ L of DNA profiling 1.PCR reaction conditions：95 DEG C 3 minutes, 95 DEG C
1 minute, 48 DEG C 1 minute, 72 DEG C 2 minutes, totally 30 circulation；72 DEG C 10 minutes.
3rd, 16S rDNA sequencings
Amplification terminates, and PCR primer is detected with 1.0% agarose gel electrophoresis, send Invitrogen (Shanghai) trade limited public affairs
Company of department is sequenced.Measure its sequence：
4th, the rose rhodochrous XHRR1 colonial morphologies of purifying aquatic water ammonia, physiological characteristic
Rose rhodochrous XHRR1 colonial morphologies, physiological characteristic see the table below 1.
The rose rhodochrous XHRR1 colonial morphologies of table 1, physiological characteristic
5th, rose rhodochrous XHRR1 identification
The bacterium 16S rDNA gene orders are compared with listed gene order in GenBank, utilized
Biolog MicroStation TM System Bacteria Identifications systems carry out bacterial strain biochemical characteristic identification respectively, and as a result display should
Bacterial strain rose rhodochrous (Rhodococcus rhodochrous).Comprehensive 16S rDNA gene sequencings, biochemical identification,
And every result such as form speciality.Assert that strain X HRR1 is rose rhodochrous (Rhodococcus rhodochrous).
Relevant information is consulted, there is no grinding with rose rhodochrous (Rhodococcus rhodochrous) purification prawn pool water body ammonia
Study carefully report.
Strain X HRR1 preservation is entitled：Rose rhodochrous XHRR1, Classification And Nomenclature are：Rhodococcus
Rhodochrous XHRR1, depositary institution are：China typical culture collection center, preservation address are：Wuhan, China, preservation
Date is：On August 3rd, 2017, deposit number are：CCTCC NO：M 2017437.
The rose rhodochrous XHRR1 of the purifying aquatic water ammonia of embodiment 3 small-scale application
1st, the growth of bacterial strain
The bacterial strain rose rhodochrous XHRR1 that embodiment 1 is obtained is by 106CFU/mL is seeded to sterilizing cultivating pool water
In, the bacteria concentration during 2~5 days is stable 108CFU/mL quantity levels, strain X HRR1 growth curve is as shown in fig. 1.
2nd, the removal effect of bacterial strain ammonia in different salinity breeding water body
Water body will be tested based on sterilizing cultivating pool water (water salinity 25), with NH4Ammonia is dense in Cl regulation water bodys
Degree adjusts water salinity as 5,15,25,35,45 as 24mg/L or so using distilled water and sea salt.The bacterium that embodiment 1 is obtained
Strain rose rhodochrous XHRR1 presses 106CFU/mL is seeded in experiment water body, in 30 DEG C, 2500~4000lx of intensity of illumination,
150~200rpm carries out shaking table culture 3 days.The changing condition of ammonia density in water body is monitored daily.As a result as shown in Fig. 2 working as water
When body salinity is 5~45, the clearance of ammonia in bacterium group water body is added to can reach 79%~99% in 3 days；When salinity reaches 25~45
The clearance of water body ammonia be more than 90%, in whole monitoring process control group (be reference by 25 test cultures system of salinity,
Wherein be not added with Rhodococcus sp XHRR1) ammonia density change it is little.It can be seen that XHRR1 bacterium have good salinity scanning, can fit
For the purification of different salinity breeding water body ammonia, in contrast, its is better for water body of the salinity more than 25.
3rd, the removal effect of bacterial strain ammonia in different temperatures breeding water body
Water body will be tested based on sterilizing cultivating pool water (water salinity 25), with NH4Ammonia is dense in Cl regulation water bodys
Degree is as 24mg/L or so.The bacterial strain rose rhodochrous XHRR1 that embodiment 1 is obtained is by 106CFU/mL is seeded to experiment water
In body, cultivation temperature is respectively set to 5 DEG C, 15 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 45 DEG C, in 2500~4000lx of intensity of illumination,
Shaking table culture is carried out under the conditions of 150~200rpm 3 days.The changing condition of ammonia density in water body is monitored daily.As a result such as Fig. 3 institutes
Show, add the clearance of ammonia in bacterium group water body to can reach more than 90% in 3 days when water temperature is 15~35 DEG C；When water temperature less than 15 DEG C or
During higher than 40 DEG C, rose rhodochrous XHRR1's is significantly affected except ammonia effect, in whole monitoring process control group (with
The test cultures system that temperature is 30 DEG C is reference, wherein being not added with Rhodococcus sp XHRR1) ammonia density change it is little.It is overall and
Speech, XHRR1 bacterial strains are suitable for breeding water body temperature conditionss during breeding production, possess good thermal adaptability, by its section
The concentration for being applied to that ammonia in water body can be effectively reduced in water body control technique link is learned, reaches the effect of Optimal culture water quality.
Influences of the strain X HRR1 of embodiment 4 to Environment of Litopenaeus vannamei Low
In the influence of Shanwei and Shenzhen cultivation base test strain to Environment of Litopenaeus vannamei Low.Breeding water body salinity is 25~28,
PH7.5~8.1,6.0~6.7mg/L of dissolved oxygen, 28~32 DEG C of temperature, water body volume are 200L.Experimental group is with 107CFU/mL concentration
Using the bacterium, control group is not added with bacterium, every group be all provided with 3 it is parallel.Test the Environment of Litopenaeus vannamei Low just a length of 6.7 ± 0.5cm of initial body, body weight
For 2.8 ± 0.2g.7 days test periods.As a result as shown in figure 4, adding bacterium group and the prawn survival rate point of control group at the end of test
Wei 92.7% and 93.3%.It can be seen that with 10 in breeding water body7When CFU/mL concentration applies the bacterium, bacterial strain is to aquaculture organism
Without obvious harmful effect.
The present invention is not limited in the range of above-mentioned specific embodiment, and the embodiment above is used for the purpose of can be to this
The use process of invention is described in detail, and has the production method of equal function and ins and outs to fall within the present invention
A part for appearance.In fact, those skilled in the art are according to description above, it becomes possible to according to each needing to find different tune
Perfect square case, these adjustment all should be in the scope of the claims by the appended claims herein.
Sequence table
<110>Nanhai Aquatic Inst., Chinese Aquatic Scientific Research Inst
Chaozhou Zheng Cheng agricultural science and technologys Co., Ltd
<120>The rose-red Rhodococcus strain XHRR1 of ammonia and its application in a kind of purifying aquatic water
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1390
<212> DNA
<213>Rose-red Rhodococcus strain XHRR1 (Rhodococcus rhodochrous XHRR1)
<400> 1
acggctccct cccacaaggg gttaggccac cggcttcggg tgttaccgac tttcatgacg 60
tgacgggcgg tgtgtacaag gcccgggaac gtattcaccg cagcgttgct gatctgcgat 120
tactagcgac tccgacttca cggggtcgag ttgcagaccc cgatccgaac tgagaccggc 180
tttaagggat tcgctccacc tcacggtatc gcagccctct gtaccgacca ttgtagcatg 240
tgtgaagccc tggacataag gggcatgatg acttgacgtc gtccccacct tcctccgagt 300
tgaccccggc agtctcctgc gagtccccac catcacgtgc tggcaacaca ggacaagggt 360
tgcgctcgtt gcgggactta acccaacatc tcacgacacg agctgacgac agccatgcac 420
cacctgtcta ccggccacaa gggaaaccac atctctgcag tcgtccggta catgtcaaac 480
ccaggtaagg ttcttcgcgt tgcatcgaat taatccacat gctccgccgc ttgtgcgggc 540
ccccgtcaat tcctttgagt tttagccttg cggccgtact ccccaggcgg ggcgcttaat 600
gcgttggcta cggcacggat cccgtggaag gaaacccaca cctagcgccc accgtttacg 660
gcgtggacta ccagggtatc taatcctgtt cgctacccac gctttcgctc ctcagcgtca 720
gttactgccc agagacccgc cttcgccacc ggtgttcctc ctgatatctg cgcatttcac 780
cgctacacca ggaattccag tctcccctgc agtactcgag tctgcccgta tcgcctgcaa 840
gcccgcagtt gagctgcggg atttcacaga cgacgcgaca aaccgcctac gagctcttta 900
cgcccagtaa ttccggacaa cgctcgcacc ctacgtatta ccgcggctgc tggcacgtag 960
ttggccggtg cttcttctcc cactaccgtc acttgcgctt cgtcatgggt gaaagaggtt 1020
tacaacccga aggccgtcat ccctcacgcg gcgtcgctgc atcaggcttg cgcccattgt 1080
gcaatattcc ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt cccagtgtgg 1140
ccggtcgccc tctcaggccg gctacccgtc gtcgccttgg taggccatta ccccaccaac 1200
aagctgatag gccgcgggct catcctgcac cgaaaaactt tccaccccag aacatgcatc 1260
ccgaggtcat atccggtatt agacccagtt tcccaggctt atcccagagt gcagggcaga 1320
tcacccacgt gttactcacc cgttcgccac taatccaccc agcaagctgg gcttcatcgt 1380
tcgactgcat 1390
Claims (2)
1. the rose-red Rhodococcus strain XHRR1 of ammonia in a kind of purifying aquatic water, it is characterized in that：Strain X HRR1 preservation
It is entitled：Rose rhodochrous XHRR1, Classification And Nomenclature are：Rhodococcus rhodochrous XHRR1, depositary institution are：
China typical culture collection center, preservation address are：Wuhan, China, preservation date are：On August 3rd, 2017, deposit number
For：CCTCC NO：M 2017437.
2. the application of rose-red Rhodococcus strain XHRR1 ammonia in purifying aquatic water described in claim 1.
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| CN111471612A (en) * | 2020-03-10 | 2020-07-31 | 中国水产科学研究院南海水产研究所深圳试验基地 | Rhodococcus ruber HDRR2Y for purifying inorganic nitrogen and phosphorus in tail water of seawater pond culture and application thereof |
| CN111471611A (en) * | 2020-03-10 | 2020-07-31 | 中国水产科学研究院南海水产研究所深圳试验基地 | Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in tail water of seawater pond culture and application thereof |
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| CN111471611A (en) * | 2020-03-10 | 2020-07-31 | 中国水产科学研究院南海水产研究所深圳试验基地 | Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in tail water of seawater pond culture and application thereof |
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