CN107810786A - A kind of disposable method of purification of simple hickory chick strain - Google Patents
A kind of disposable method of purification of simple hickory chick strain Download PDFInfo
- Publication number
- CN107810786A CN107810786A CN201710999300.9A CN201710999300A CN107810786A CN 107810786 A CN107810786 A CN 107810786A CN 201710999300 A CN201710999300 A CN 201710999300A CN 107810786 A CN107810786 A CN 107810786A
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- CN
- China
- Prior art keywords
- culture tube
- mycelia
- purification
- hickory chick
- sterile
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Abstract
The invention discloses a kind of disposable method of purification of simple hickory chick strain, belongs to hickory chick production technical field, and the present invention includes step, the first step：The culture tube with two ports is prepared, two ports of the culture tube are equipped with Ventilative bottle plug, and two ports of the culture tube are mutually communicated；Second step：Sterilization treatment is carried out to culture tube；3rd step：After culture tube cooling after processing subject to sterilization, from a port of culture tube access spore, mycelia or compost containing spore or mycelia；4th step：Culture tube is cultivated, sterile mycelia is obtained from another passage port of culture tube or directly inserts another port of culture tube in sterile soil and obtain sterile mycelia.Simple and fast of the present invention and can be sterile, the step of purification ＆ isolation one are completed.The advantages of production strain industry level overall with lifting hickory chick food.Incubation is greatly simplified, improves production efficiency.
Description
Technical field
The invention belongs to hickory chick production technical field, is disposably carried more particularly to a kind of simple hickory chick strain
Pure method.
Background technology
Current purification technique, is purified using test tube, and entrance and the product after purification of raw material are from same
Individual mouth, which enters, to be gone out with same mouth, it is necessary to stringent sterilization and strict aseptic technique, it is necessary to is operated in an aseptic environment, it is necessary to be made
With the equipment such as desinfection chamber and superclean bench or Biohazard Safety Equipment, it is necessary to which certain professional knowledge is, it is necessary to which operator receives specially
The training of industry, the requirement to operator is higher, and sterile equipment is used so that cost for purification greatly improves；Current purification technique
In, hickory chick needs to sterilize or what needs were fine takes kind of a method so that success rate is extremely low, and general skilled operation personnel take explant
Body success rate is also only in 20-30%；Purification and rejuvenation need repeatedly switching, it is necessary to which repeatedly switching is also serious while extensive work
Influence the activity of hickory chick strain.
The content of the invention
It is an object of the invention to：In order to solve current purification technique it is complicated, it is higher to equipment requirement, to operating personnel will
Ask higher and the problem of purification efficiency is not high, the present invention provides a kind of simple hickory chick strain disposable method of purification.
The present invention specifically uses following technical scheme to achieve these goals：
The simple disposable method of purification of hickory chick strain of the present invention, it is characterised in that comprise the following steps：
The first step：The culture tube with two ports is prepared, two ports of the culture tube are equipped with Ventilative bottle plug, institute
Two ports for stating culture tube are mutually communicated；
Second step：Culture medium is put into culture tube and carries out sterilization treatment；
3rd step：After culture tube cooling after processing subject to sterilization, from a port of culture tube access spore, mycelia or
Compost containing spore or mycelia；
4th step：Culture tube is cultivated, sterile mycelia is obtained from another passage port of culture tube or will directly train
Another port of pipe is supported inserted with obtaining sterile mycelia in another port after a period of time in soil bacteria.
Preferably, the culture tube is " U "-shaped, linear, waveform or V-arrangement.
Preferably, the Biocidal treatment method is normal pressure or autoclaving.
Beneficial effects of the present invention are as follows：
The present invention disposably can be directly inoculated with non-aseptic state, it is only necessary to a simple siphunculus equipment without
The desinfection chamber either complex device such as superclean bench or Biohazard Safety Equipment is wanted, operating personnel can be without any professional knowledge
In the case of complete purification task, only once purificate and rejuvenate after inoculation, without switching, greatly simplify incubation, improve life
Efficiency is produced, there is the advantages of lifting hickory chick entirety strain level of industry.
Brief description of the drawings
Fig. 1 is the structural representation of culture tube；
Reference：1- Ventilative bottle plugs, 2- culture tubes.
Embodiment
In order to which those skilled in the art are better understood from the present invention, below in conjunction with the accompanying drawings with following examples to the present invention
It is described in further detail.
The simple disposable method of purification of hickory chick strain of the present invention, comprises the following steps：
The first step：The culture tube 2 with two ports is prepared, two ports of the culture tube 2 are equipped with Ventilative bottle plug
1, two ports of the culture tube 2 are mutually communicated；
Second step：Culture medium is put into culture tube 2 and carries out sterilization treatment；
3rd step：After culture tube 2 after processing subject to sterilization cools down, from the port access spore of culture tube 2, mycelia or
Person contains spore or the compost of mycelia；
4th step：Culture tube 2 is cultivated, sterile mycelia is obtained from another passage port of culture tube 2 or directly will
Another port of culture tube 2 is inserted with obtaining sterile mycelia in soil bacteria.
Preferably, the culture tube 2 is " U "-shaped, linear, waveform or V-arrangement.
Preferably, the Biocidal treatment method is normal pressure or autoclaving.
Disinfecting action is directly carried out after putting culture into culture tube, after cooling subject to sterilization, is accessed from one end of culture tube
Spore, mycelia, or the compost containing spore or mycelia, after cultivating one end time under 5-18 degrees Celsius of low temperature environment, from
The other end of culture tube access spore can directly take out the sterile mycelia purified.Its principle is, at low temperature, hickory chick bacterium
The silk speed of growth is more than varied bacteria growing speed, and using this principle, hickory chick strain preferentially reaches the other end of culture tube, culture medium
It is sterile again, so the mycelium taken out from the other end of culture tube access spore is then sterile mycelium, and because at first
The mycelia for reaching the other end is that the speed of growth is most fast and be that most strong can reach the purpose disposably purified.
Described above, only presently preferred embodiments of the present invention is not intended to limit the invention, patent protection model of the invention
Enclose and be defined by claims, the equivalent structure change that every specification and accompanying drawing content with the present invention is made, similarly
It should include within the scope of the present invention.
Claims (3)
1. the disposable method of purification of a kind of simple hickory chick strain, it is characterised in that comprise the following steps：
The first step：The culture tube (2) with two ports is prepared, two ports of the culture tube (2) are equipped with Ventilative bottle plug
(1), two ports of the culture tube (2) are mutually communicated；
Second step：Culture medium is put into culture tube (2) and carries out sterilization treatment；
3rd step：After culture tube (2) cooling after processing subject to sterilization, from a port of culture tube (2) access spore, mycelia or
Person contains spore or the compost of mycelia；
4th step：Culture tube (2) is cultivated, sterile mycelia is obtained from another passage port of culture tube (2) or directly will
Another port of culture tube (2) inserts in sterile soil and obtains sterile mycelia.
2. the disposable method of purification of simple hickory chick strain according to claim 1, it is characterised in that the culture tube
(2) " U "-shaped, linear, waveform or " V " shape.
3. the disposable method of purification of simple hickory chick strain according to claim 1, it is characterised in that the sterilization treatment
Method is normal pressure or autoclaving.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710999300.9A CN107810786A (en) | 2017-10-20 | 2017-10-20 | A kind of disposable method of purification of simple hickory chick strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710999300.9A CN107810786A (en) | 2017-10-20 | 2017-10-20 | A kind of disposable method of purification of simple hickory chick strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107810786A true CN107810786A (en) | 2018-03-20 |
Family
ID=61608363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710999300.9A Pending CN107810786A (en) | 2017-10-20 | 2017-10-20 | A kind of disposable method of purification of simple hickory chick strain |
Country Status (1)
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|---|---|
| CN (1) | CN107810786A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687639A (en) * | 2011-12-19 | 2012-09-26 | 河南科技大学 | Method for separating Morchella strains |
| CN106282034A (en) * | 2016-08-30 | 2017-01-04 | 杨燕 | A kind of Morchella esculenta (L.) Pers strain fast breeding method |
| CN107129938A (en) * | 2017-07-06 | 2017-09-05 | 江油市静远食用菌科技有限责任公司 | It is a kind of to improve the method that morchella mother culture manufactures success rate |
| CN107177514A (en) * | 2017-07-06 | 2017-09-19 | 江油市静远食用菌科技有限责任公司 | A kind of purity is the preparation method of 100% morchella mother culture |
-
2017
- 2017-10-20 CN CN201710999300.9A patent/CN107810786A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687639A (en) * | 2011-12-19 | 2012-09-26 | 河南科技大学 | Method for separating Morchella strains |
| CN106282034A (en) * | 2016-08-30 | 2017-01-04 | 杨燕 | A kind of Morchella esculenta (L.) Pers strain fast breeding method |
| CN107129938A (en) * | 2017-07-06 | 2017-09-05 | 江油市静远食用菌科技有限责任公司 | It is a kind of to improve the method that morchella mother culture manufactures success rate |
| CN107177514A (en) * | 2017-07-06 | 2017-09-19 | 江油市静远食用菌科技有限责任公司 | A kind of purity is the preparation method of 100% morchella mother culture |
Non-Patent Citations (1)
| Title |
|---|
| 陕西省科学技术委员会: "《食用菌制种及栽培技术》", 30 December 2001 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180320 |
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| RJ01 | Rejection of invention patent application after publication |