CN107801980B - Prepare that albumen gets blisters the method for powder and the albumen that is prepared by this method is got blisters powder - Google Patents
Prepare that albumen gets blisters the method for powder and the albumen that is prepared by this method is got blisters powder Download PDFInfo
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- CN107801980B CN107801980B CN201710972252.4A CN201710972252A CN107801980B CN 107801980 B CN107801980 B CN 107801980B CN 201710972252 A CN201710972252 A CN 201710972252A CN 107801980 B CN107801980 B CN 107801980B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
- A23L29/281—Proteins, e.g. gelatin or collagen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/269—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
- A23L29/271—Curdlan; beta-1-3 glucan; Polysaccharides produced by agrobacterium or alcaligenes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
- A23L29/281—Proteins, e.g. gelatin or collagen
- A23L29/284—Gelatin; Collagen
Abstract
It gets blisters powder and preparation method thereof the invention discloses a kind of albumen.The method includes：(1) polysaccharide gum solution is prepared, phosphate solution is carried out first with the polysaccharide gum solution to react, and obtains the first Phosphorylated products；(2) gelatin solution is prepared, then carrying out second with the first Phosphorylated products reacts, and obtains the second Phosphorylated products；(3) pH value of the second Phosphorylated products is adjusted to 56, is then centrifuged, is collected supernatant；(4) supernatant is subjected to hyperfiltration treatment and spray drying successively, wherein it is described first reaction condition include：PH value is 68, and temperature is 35 65 DEG C, and the time is 0.5 5 hours；It is described second reaction condition include：PH value is 7.5 10.5, and temperature is 35 65 DEG C, and the time is 0.5 5 hours.The albumen prepared by the present invention gets blisters powder with higher emulsibility and foaming characteristic, and the foam stability for carrying out mechanical acquisition of getting blisters is higher.
Description
Technical field
The present invention relates to field of food, and in particular to it is a kind of prepare albumen get blisters powder method and the egg that is prepared by this method
Powder is steeped in game of shuttlecocks.
Background technology
The powder of getting blisters prepared based on albumen (such as albumen, soybean protein, casein) is widely used in cake, ice
River in Henan Province leaching etc. is dismissed in food.These powder of getting blisters not only influence food sense organ in the processing and production of food, and are adding to food
Physicochemical characteristics in work process, manufacturing process and preserving process also plays a very important role.Based on albumen system
Standby powder of getting blisters is that a kind of common food is got blisters powder, but also there is the shortcomings of expensive, cholesterol level is high.Gelatin can
Using as stabilizer, thickener, adhesive and foaming agent etc. be widely used in improving in food manufacturing the elasticity of food, viscosity and
And stability etc..But compared with other surfaces activating agent (such as lactalbumin, soybean protein, Arabic gum), gelatin table
Reveal weaker emulsibility and foaming characteristic, is limited to dismiss the application in food.
Research finds that the protein-PS colloid system formed using electrostatic interaction is showed than simple albumen, polysaccharide gum
Go out higher emulsibility and foaming characteristic.But it is still vulnerable to the influence of pH, salinity, temperature.Therefore, how to prepare stable
Protein-PS colloid is tied to form as current an one of research hotspot.
Phosphorylation modification can improve the functional characteristic of object.In general, phosphate can be with-OH under neutrallty condition
It reacts, phosphate can be with the lysine in the protein ,-NH on serine under alkaline condition2Reaction, the phosphoric acid foundation of introducing
Group can improve the surface hydrophobic of protein, show higher emulsibility and foaming characteristic.
It discloses in the application of Publication No. CN1300562A and is dispersed in the natural of emulsifying capacity comprising at least one
Liposoluble in the matrix of the mixture of polysaccharide gum or the sizing material and/or protein or protein mixture with emulsifying capacity
The powder composition of property vitamin.Although the powder composition has emulsibility, its foaming characteristic is weaker, and it is easy
It is influenced by pH, salinity, temperature etc., stability is not high, it is caused to be limited to dismiss the application in food.
Invention content
The purpose of the invention is to overcome, the existing powder of getting blisters prepared based on albumen is expensive, cholesterol contains
It measures the shortcomings of high, gelatin based food additive shows weaker emulsibility and foaming characteristic, it is caused to be limited to dismiss in food
Application the defects of, a kind of albumen is provided and is got blisters powder and preparation method thereof, albumen powder of getting blisters introduces phosphoric acid in preparation process
Foundation group effectively increases the surface hydrophobic of gelatin, and albumen obtained by this method powder of getting blisters is made to show higher emulsification
Property and foaming characteristic, and by the albumen get blisters powder machinery get blisters acquisition foam have higher stability.
To achieve the goals above, one aspect of the present invention provides a kind of albumen for preparing and gets blisters the method for powder, the method packet
Include following steps：
(1) polysaccharide gum solution is prepared, phosphate solution is carried out first with the polysaccharide gum solution to react, and obtains the first phosphorus
Acidizing product；
(2) gelatin solution is prepared, the first Phosphorylated products for then being obtained with step (1) carry out second and react, and obtain the
Diphosphate product；
(3) then the pH value for the second Phosphorylated products that regulating step (2) obtains is centrifuged, to 5-6 in collection
Clear liquid；
(4) supernatant that step (3) obtains is subjected to hyperfiltration treatment and spray drying successively.
Second aspect of the present invention provides the albumen that is prepared by preceding method and gets blisters powder.
The present inventor has found by in-depth study, by introducing phosphate radical in getting blisters powder preparation process in albumen
Group can utilize polysaccharide gum solution and protein solution to be made bright using technologies such as substep phosphorylation and membrane separating and purifyings
Glue-phosphate-polysaccharide glue composition, effectively increases the surface hydrophobic of gelatin, and albumen obtained by this method is made to get blisters powder
Show higher emulsibility and foaming characteristic.It gets blisters powder, makes in addition, the present invention prepares the albumen using the method for spray drying
Albumen obtained by this method get blisters powder using machinery get blisters acquisition foam have higher stability.
Further, albumen provided by the present invention get blisters powder preparation method it is easy to operate, preparation process is simply controllable,
The albumen that can be applied to extensive mass industrial production, and produce product foaming characteristic of getting blisters is preferable, can be widely used in and beat
Send out field of food.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific implementation mode
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
As previously mentioned, the present invention provides a kind of albumen for preparing to get blisters the method for powder, the described method comprises the following steps：
(1) polysaccharide gum solution is prepared, phosphate solution is carried out first with the polysaccharide gum solution to react, and obtains the first phosphorus
Acidizing product；
(2) gelatin solution is prepared, the first Phosphorylated products for then being obtained with step (1) carry out second and react, and obtain the
Diphosphate product；
(3) then the pH value for the second Phosphorylated products that regulating step (2) obtains is centrifuged, to 5-6 in collection
Clear liquid；
(4) supernatant that step (3) obtains is subjected to hyperfiltration treatment and spray drying successively.
According to the present invention, in the above-mentioned methods, mainly by carrying out phosphorylation modification to polysaccharide gum and gelatin successively step by step
Processing, phosphate groups are introduced in preparation process, so that polysaccharide gum is passed through phosphate groups with gelatin and connects, be formed in weak acid ring
The compound that property is stablized under border, while being effectively improved foaming characteristic and the emulsification of the one-component of simple gelatin or polysaccharide gum
Property.In addition, technologies, the albumen for the stabilization that can be purified such as membrane separating and purifying and spray drying is utilized to beat in the above-mentioned methods
Steep powder.
According to the present invention, phosphorylation modification processing can improve the functional characteristic of object.In general, under neutrallty condition
Phosphate can be reacted with the hydroxyl (- OH) in polysaccharide gum, and phosphate can be with lysine, the silk ammonia in gelatin under alkaline condition
Amido (- NH on acid2) reaction, the phosphate groups connection polysaccharide gum and gelatin of introducing can significantly improve simple gelatin list
The surface hydrophobic of one component, while being effectively improved the elasticity and viscosity of polysaccharide gum, make compound features go out higher emulsibility,
Foaming characteristic, elasticity, viscosity and stability.
According to the present invention, phosphate can be reacted with the hydroxyl (- OH) in polysaccharide gum under neutrallty condition, therefore, in order to make
The polysaccharide gum fully dissolves, and obtains phosphorylation modification to the maximum extent, and the condition of first reaction preferably includes：PH value
For 6-7.5, treatment temperature is 40-60 DEG C, and processing time is 0.5-2 hours.
According to the present invention, phosphate can be with the lysine in gelatin, the amido (- NH on serine under alkaline condition2)
Reaction, further, since gelatin dissolves in hot water, therefore, in order to make the gelatin fully dissolve, and to the maximum extent with above-mentioned phosphorus
The phosphate groups reaction being acidified in the polysaccharide gum of modification, and then the gelatin-compounded object of polysaccharide gum-phosphate groups-is obtained, it is described
The condition of second reaction preferably includes：PH value is 8-10, and treatment temperature is 40-60 DEG C, and processing time is 0.5-2 hours.
According to the present invention, the dosage of the polysaccharide gum solution, the phosphate solution and institute's gelatine solution can basis
The amido (- NH contained by hydroxyl (- OH) quantity, phosphatic coordination ability and gelatin contained by specifically used polysaccharide gum2) number
Amount and the concentration of each material solution are selected, for example, in the polysaccharide gum, the phosphate solution in the polysaccharide gum solution
Phosphate and the gelatin in institute's gelatine solution dosage weight ratio can be 15：1：15~20：1：20.
According to the present invention, in step (3), the condition of the centrifugation can be produced according to the second phosphorylation actually obtained
The molecular weight of object is selected, for example, the condition of the centrifugation may include：Rotating speed is 7000-9000rpm, centrifugation point
From time be 10-20 minutes.
According to the present invention, in step (4), for purification of target product, make the albumen finally obtained get blisters powder have it is optimal
Foaming characteristic, emulsibility, elasticity, viscosity and stability, the condition of the hyperfiltration treatment preferably includes：It is using molecular cut off
The ultrafiltration membrane of 4500-5500Da carries out hyperfiltration treatment, and tangential flow velocity is 4-12L/ (minm2), pressure 60-70psi.
According to the present invention, in step (4), temperature is higher in order to prevent or the drying time long denaturation for causing gelatin, this
In invention by the way of spray drying to abovementioned steps in the trapped fluid that obtains be dried, it is preferable that the spray drying
Condition include：Inlet air temperature is 180-190 DEG C, and leaving air temp is 80-100 DEG C.
According to the present invention, the gelatin is the egg derived by fibrous collagen from animal and with emulsifying capacity
White matter, the gelatin can interrupt structure simultaneously through acid or basic hydrolysis or enzymolysis and extraction by hide or poultry bone in extraction process respectively
Part solution supination, you can obtaining can hot water soluble gelatin.The gelatin can also be including its applicable chemical derivative for example
Acetylated gelatin or cross-linked gelatin.
According to the present invention, it is contemplated that bovine brain spongiform disease and some religious beliefs, cattle hide gelatin and pigskin gelatin are eaten again
Application in product material has certain limitation, therefore fishskin gelatin becomes a kind of suitable substitute, in the present invention, institute
It is preferably fishskin gelatin to state glue clearly.The content of nonpolar amino acid, described in the complexity and gelatin that are obtained in view of gelatin
Gelatin is more preferably tilapia skin gelatin, when the gelatin is tilapia skin gelatin, nonpolar amino in the gelatin
For the content of acid up to 80 moles of % or more, these nonpolar amino acids can further increase emulsibility and the blistering of target product
Property.
According to the present invention, the polysaccharide gum is the natural polysaccharide glue with emulsifying capacity, the day with emulsifying capacity
Right polysaccharide gum refer to preferably in the present invention be derived from plant, animal or microorganism, and without purposive chemical modification and
Change the polysaccharide gum of chemical constitution.All sizing materials such as exudate glue, algin, seed glue or microbiological gum, if they have
Emulsifying capacity then can be adopted to the implementation present invention, and according to desired use, these glue will at least be suitable for food addition or preferred
Ground can be used as the substance of food through various global regulatory authorities authorizations.
According to the present invention, the polysaccharide gum can be Arabic gum, flaxseed gum, ghatti gum, tamarind gum and Arab
At least one of at least one of galactan, preferably Arabic gum, ghatti gum and arabogalactan, most preferably
For Arabic gum.These polysaccharide gums have enough emulsifications, they can reduce the interfacial tension in oil-in-water emulsion, simultaneously
The viscosity of water phase can be improved again, especially when the polysaccharide gum is Arabic gum, do not generate heat substantially in nutrition, also
Containing a large amount of water-soluble dietary fiber, the requirement of composite heal-care food, while food mouthfeel can be enhanced.
According to the present invention, for the ease of in the hydroxyl (- OH) and gelatin in the phosphate and polysaccharide gum amido (-
NH2) connection, the phosphate preferably have multiple phosphate groups, for example, the phosphate can be pyrophosphate, four
At least one of metaphosphate and polyphosphate, preferably pyrophosphate solution, most preferably sodium pyrophosphate solution.
It gets blisters powder as previously mentioned, second aspect of the present invention additionally provides the albumen prepared by preceding method.
Albumen obtained by this method is set to beat since the albumen gets blisters powder using preceding method preparation according to the present invention
Steeping powder has excellent surface hydrophobic, shows higher emulsibility and foaming characteristic, and the albumen gets blisters powder using machine
The get blisters foam of acquisition of tool has higher stability, specifically, is carried out machinery by albumen powder of getting blisters and is got blisters the bubble of acquisition
The fissipation factor tan δ of foam are no more than 40, preferably 10-40.
According to the present invention, the fissipation factor tan δ are obtained by using the frequency scanning in rheology, under normal circumstances,
Albumen powder of getting blisters is carried out by rheology test is carried out using rheometer using the get blisters foam of acquisition of machinery, when test, stream
Become the strain (or stress) that instrument applies a sine wave rule to sample first, sample can feed back the stress of a sine wave rule
(or strain), between two sine waves can there are one the sizes of phase difference δ, δ between 0-90 °, for perfect fluid, δ is
90 °, for ideal solid, δ is 0 °, has viscoelastic actual sample, δ is between 0-90 °, tan δ>1, it was demonstrated that sample glues
Property account for major part, be fluid, the tan δ values are smaller, and the stability for representing the fluid is better.In the present invention, described to sweep
The frequency retouched is 1Hz, and the albumen is got blisters, and to carry out the rotating speed of getting blisters that the machinery got blisters of machinery is got blisters be 12000g, whipping time to powder
For 2min.
The present invention will be described in detail by way of examples below.
In following embodiment and comparative example, Arabic gum is the commercially available product of Lai Kerui companies, total ash≤4%, acid
Insoluble ash≤0.5%, acid non-soluble substance≤1% (butt meter)；
In following embodiment and comparative example, flaxseed gum is purchased from Zhengzhou Kang Ben Science and Technology Ltd.s；
In following embodiment and comparative example, tilapia skin gelatin is purchased from Hua Chang high-techs company；
In following embodiment and comparative example, gadus skin gelatin is purchased from Hua Chang high-techs company；
In following embodiment and comparative example, other used reagents are analytical reagents；
In following embodiment and comparative example, albumen obtained get blisters powder carry out machinery get blisters acquisition foam loss because
Sub- tan δ are measured by the frequency scanning method in rheology, and the frequency of scanning is 1Hz.
Embodiment 1
(1) Arabic gum is dissolved in deionized water, to dissolving, compound concentration is 4% (w/ for stirring under 50 DEG C of water-baths
V) gumwater, and use the pH value of the HCl solution of a concentration of 1mol/L adjusting gumwater to 7.Take 60mL
The gumwater that the pH value is 7, is added sodium pyrophosphate, makes a concentration of 0.25% (w/v) of sodium pyrophosphate, room thereto
The lower stirring of temperature under conditions of being then 7 by previous reaction liquid holding pH value, is placed in the water that temperature is 50 DEG C to be sufficiently mixed uniformly
Heating stirring 1h in bath carries out the first reaction, is cooled to room temperature after reaction, obtains the first Phosphorylated products, for use.
(2) tilapia skin gelatin is dissolved in deionized water, stirring extremely dissolving, compound concentration are under 50 DEG C of water-baths
The tilapia skin gelatin solution of 4% (w/v), and use the pH value of the NaOH solution of a concentration of 1mol/L adjusting gumwater
To 9.The tilapia skin gelatin solution that the pH value is 9 is pressed into V with the first Phosphorylated products prepared in step (1)Isinglass：
VFirst Phosphorylated products=1:1 ratio is uniformly mixed, and the HCl solution of NaOH solution and 1mol/L that 1mol/L is used in combination adjusts pH value to 9.
Under conditions of being then 9 by previous reaction liquid holding pH value, it is placed in heating stirring 1h in the water-bath that temperature is 50 DEG C, carries out second
Reaction, is cooled to room temperature, obtains the second Phosphorylated products after reaction.
(3) by the second Phosphorylated products of aforementioned the preparation NaOH solution of 1mol/L and the HCl solution tune pH of 1mol/L
It is worth to 5.5.8000rpm centrifuges 15min at room temperature, collects supernatant.
(4) it uses molecular cut off to be isolated and purified for the ultrafiltration membrane of 5000Da the supernatant that step (3) is collected, cuts
It is 8L (minm to flow velocity2), pressure 60psi.It collects the trapped fluid that ultrafiltration obtains to be spray-dried, wherein inlet air temperature
182 DEG C, 90 DEG C, flow 0.9L/h of leaving air temp obtains albumen and gets blisters powder P1.
Embodiment 2
(1) Arabic gum is dissolved in deionized water, to dissolving, compound concentration is 4% (w/ for stirring under 50 DEG C of water-baths
V) gumwater, and use the pH value of the HCl solution of a concentration of 1mol/L adjusting gumwater to 7.2.It takes
The gumwater that pH value described in 60mL is 7.2, is added sodium pyrophosphate, makes a concentration of the 0.25% of sodium pyrophosphate thereto
(w/v), it stirs at room temperature to be sufficiently mixed uniformly, under conditions of being then 7.2 by previous reaction liquid holding pH value, is placed in temperature
For heating stirring 0.5h in 50 DEG C of water-baths, the first reaction is carried out, is cooled to room temperature after reaction, obtains the production of the first phosphorylation
Object, for use.
(2) tilapia skin gelatin is dissolved in deionized water, stirring extremely dissolving, compound concentration are under 55 DEG C of water-baths
The tilapia skin gelatin solution of 4% (w/v), and use the pH value of the NaOH solution of a concentration of 1mol/L adjusting gumwater
To 9.5.The tilapia skin gelatin solution that the pH value is 9.5 is pressed into V with the first Phosphorylated products prepared in step (1)Isinglass：
VFirst Phosphorylated products=3:1 ratio is uniformly mixed, be used in combination 1mol/L NaOH solution and 1mol/L HCl solution adjusting pH value to
9.5.Under conditions of being then 9.5 by previous reaction liquid holding pH value, it is placed in heating stirring 0.5h in the water-bath that temperature is 55 DEG C,
The second reaction is carried out, is cooled to room temperature after reaction, obtains the second Phosphorylated products.
(3) by the second Phosphorylated products of aforementioned the preparation NaOH solution of 1mol/L and the HCl solution tune pH of 1mol/L
It is worth to 6.7000rpm centrifuges 20min at room temperature, collects supernatant.
(4) it uses molecular cut off to be isolated and purified for the ultrafiltration membrane of 4800Da the supernatant that step (3) is collected, cuts
It is 10L (minm to flow velocity2), pressure 60psi.It collects the trapped fluid that ultrafiltration obtains to be spray-dried, wherein into wind-warm syndrome
182 DEG C of degree, 95 DEG C, flow 1L/h of leaving air temp obtain albumen and get blisters powder P2.
Embodiment 3
(1) Arabic gum is dissolved in deionized water, to dissolving, compound concentration is 4% (w/ for stirring under 45 DEG C of water-baths
V) gumwater, and use the pH value of the HCl solution of a concentration of 1mol/L adjusting gumwater to 6.8.It takes
The gumwater that pH value described in 60mL is 6.8, is added sodium pyrophosphate, makes a concentration of the 0.25% of sodium pyrophosphate thereto
(w/v), it stirs at room temperature to be sufficiently mixed uniformly, under conditions of being then 6.8 by previous reaction liquid holding pH value, is placed in temperature
For heating stirring 2h in 45 DEG C of water-baths, the first reaction is carried out, is cooled to room temperature after reaction, obtains the production of the first phosphorylation
Object, for use.
(2) tilapia skin gelatin is dissolved in deionized water, stirring extremely dissolving, compound concentration are under 60 DEG C of water-baths
The tilapia skin gelatin solution of 4% (w/v), and use the pH value of the NaOH solution of a concentration of 1mol/L adjusting gumwater
To 8.5.The tilapia skin gelatin solution that the pH value is 8.5 is pressed into V with the first Phosphorylated products prepared in step (1)Isinglass：
VFirst Phosphorylated products=1:3 ratio is uniformly mixed, be used in combination 1mol/L NaOH solution and 1mol/L HCl solution adjusting pH value to
8.5.Under conditions of being then 8.5 by previous reaction liquid holding pH value, it is placed in heating stirring 2h in the water-bath that temperature is 60 DEG C, into
Row second reacts, and is cooled to room temperature after reaction, obtains the second Phosphorylated products.
(3) by the second Phosphorylated products of aforementioned the preparation NaOH solution of 1mol/L and the HCl solution tune pH of 1mol/L
It is worth to 5.9000rpm centrifuges 10min at room temperature, collects supernatant.
(4) it uses molecular cut off to be isolated and purified for the ultrafiltration membrane of 5200Da the supernatant that step (3) is collected, cuts
It is 12L (minm to flow velocity2), pressure 60psi.It collects the trapped fluid that ultrafiltration obtains to be spray-dried, wherein into wind-warm syndrome
182 DEG C of degree, 85 DEG C, flow 0.8L/h of leaving air temp obtain albumen and get blisters powder P3.
Embodiment 4
According to the method for embodiment 1, unlike, the gelatin is gadus skin gelatin, finally obtains albumen and gets blisters powder P4.
Embodiment 5
According to the method for embodiment 1, unlike, the polysaccharide gum is flaxseed gum, finally obtains albumen and gets blisters powder P5.
Embodiment 6
According to the method for embodiment 1, unlike, the phosphate is four sodium metaphosphates, finally obtains albumen and gets blisters powder
P6。
Comparative example 1
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during phosphate is not used, directly
Gumwater after adjusting pH value is reacted with tilapia skin gelatin solution, is formed finally using electrostatic interaction
Albumen get blisters powder D1.
Comparative example 2
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during, the pH of first reaction
Value is 4, finally obtains albumen and gets blisters powder D2.
Comparative example 3
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during, the pH of first reaction
Value is 12, finally obtains albumen and gets blisters powder D3.
Comparative example 4
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during, the pH of second reaction
Value is 3, finally obtains albumen and gets blisters powder D4.
Comparative example 5
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during, use sucrose fatty ester
The Arabic gum is substituted, the tilapia skin gelatin is substituted using soybean protein, albumen is finally obtained and gets blisters powder D5.
Comparative example 6
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during, do not use hyperfiltration treatment, most
Albumen is obtained eventually to get blisters powder D6.
Comparative example 7
According to the method for embodiment 1, unlike, prepare albumen get blisters powder during, using spray drying, and
It is the dry 5h in temperature is 200 DEG C of baking oven, finally obtains albumen and get blisters powder D7.
Test case 1
This test case is used to illustrate that albumen to be got blisters the foamability and stability of powder P1
(1) test of foamability：0.1g albumen is got blisters water-oil mixed liquor (V of powder P1 and 20mLWater：VOil=9:1) it mixes
It closes, carrying out machinery using high-speed tissue mashing machine with the rotating speed of 12000g gets blisters 2min, is transferred to rapidly in 100mL graduated cylinders, and survey
Determine foam height (cylinder scale：ML), for indicate albumen get blisters powder P1 foamability size, test result such as 1 institute of table
Show.
(2) get blisters 0.1g albumen water-oil mixed liquor (V of powder P1 and 20mLWater：VOil=9:1) it mixes, uses high-speed organization
Bruisher carries out the machinery 2min that gets blisters with the rotating speed of 12000g and tests its damage using the frequency scanning in rheology after standing 2h
Consumption factor t an δ foams are tested (scan frequency 1Hz), and test result is as shown in table 1.
Test case 2-6
Machinery is carried out according to the method for EXPERIMENTAL EXAMPLE 1 to get blisters and the foamability and fissipation factor of the foam to obtaining
Tan δ are tested, unlike, get blisters powder P2-P6 of albumen is respectively adopted and carries out machinery and gets blisters, test result is as shown in table 1.
Contrast test example 1-7
Machinery is carried out according to the method for EXPERIMENTAL EXAMPLE 1 to get blisters and the foamability and fissipation factor of the foam to obtaining
Tan δ are tested, unlike, get blisters powder D1-D7 of albumen is respectively adopted and carries out machinery and gets blisters, test result is as shown in table 1.
Table 1
| Number | Albumen is got blisters powder | Foamability (mL) | Stability (tan δ) |
| Embodiment 1 | P1 | 98.7 | 13±1.71 |
| Embodiment 2 | P2 | 97.2 | 34±1.23 |
| Embodiment 3 | P3 | 96.5 | 16±2.54 |
| Embodiment 4 | P4 | 88.8 | 35±1.85 |
| Embodiment 5 | P5 | 87.6 | 37±1.44 |
| Embodiment 6 | P6 | 88.5 | 36±2.35 |
| Comparative example 1 | D1 | 70.1 | 52±2.74 |
| Comparative example 2 | D2 | 73.2 | 46±1.53 |
| Comparative example 3 | D3 | 84,3 | 40±2.12 |
| Comparative example 4 | D4 | 72.3 | 48±1.64 |
| Comparative example 5 | D5 | 68.4 | 52±1.78 |
| Comparative example 6 | D6 | 70.4 | 45±2.32 |
| Comparative example 7 | D7 | 62.8 | 54±1.71 |
Albumen prepared by method using the present invention powder of getting blisters, which is can be seen that, by the result of table 1 shows higher breast
The property changed and foaming characteristic, albumen made from the method by the present invention get blisters powder carry out machinery get blisters acquisition foam have it is higher steady
It is qualitative.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In the skill of the present invention
In art conception range, technical scheme of the present invention can be carried out a variety of simple variants, including each technical characteristic with it is any its
Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, belongs to
Protection scope of the present invention.
Claims (12)
1. a kind of albumen for preparing is got blisters the method for powder, which is characterized in that the described method comprises the following steps：
（1）Polysaccharide gum solution is prepared, phosphate solution is carried out first with the polysaccharide gum solution to react, and obtains the first phosphorylation
Product；
（2）Prepare gelatin solution, then with step（1）The first obtained Phosphorylated products carry out the second reaction, obtain the second phosphorus
Acidizing product；
（3）Regulating step（2）Then the pH value of the second obtained Phosphorylated products is centrifuged to 5-6, collect supernatant
Liquid；
（4）By step（3）Obtained supernatant carries out hyperfiltration treatment and spray drying successively,
Wherein, the condition of first reaction includes：PH value is 6-8, and temperature is 35-65 DEG C, and the time is 0.5-5 hours；It is described
Second reaction condition include：PH value is 7.5-10.5, and temperature is 35-65 DEG C, and the time is 0.5-5 hours, and the gelatin is fish
Skin gelatin, the polysaccharide gum are one kind in Arabic gum, flaxseed gum, ghatti gum, tamarind gum and arabogalactan,
The phosphate is one kind in pyrophosphate, tetrametaphosphate and polyphosphate,
Step（4）In, the condition of the hyperfiltration treatment includes：It is carried out using the ultrafiltration membrane that molecular cut off is 4500-5500Da
Hyperfiltration treatment, tangential flow velocity are 4-12L/ (minm2), pressure 60-70psi.
2. according to the method described in claim 1, wherein, the condition of first reaction includes：PH value is 6-7.5, and temperature is
40-60 DEG C, the time is 0.5-2 hours；It is described second reaction condition include：PH value is 8-10, and temperature is 40-60 DEG C, the time
It is 0.5-2 hours.
3. according to the method described in claim 1, wherein, in the polysaccharide gum, the phosphate solution in the polysaccharide gum solution
Phosphate and the gelatin dosage in institute's gelatine solution weight ratio be 15：1：15 ~ 20：1：20.
4. according to the method described in claim 1, wherein, step（3）In, the condition of the centrifugation includes：Rotating speed is
The time of 7000-9000rpm, centrifugation are 10-20 minutes.
5. according to the method described in claim 1, wherein, step（4）In, the condition of the spray drying includes：Inlet air temperature
It it is 180-190 DEG C, leaving air temp is 80-100 DEG C.
6. according to the method described in any one of claim 1-5, wherein the gelatin is tilapia skin gelatin.
7. according to the method described in any one of claim 1-5, wherein the polysaccharide gum is Arabic gum.
8. according to the method described in any one of claim 1-5, wherein the phosphate is pyrophosphate.
9. according to the method described in claim 8, wherein, the phosphate is sodium pyrophosphate.
The powder 10. albumen prepared by the method described in any one of claim 1-9 is got blisters.
The powder 11. albumen according to claim 10 is got blisters, wherein carried out machinery by albumen powder of getting blisters and got blisters acquisition
The fissipation factor tan δ of foam are no more than 40.
The powder 12. albumen according to claim 11 is got blisters, wherein carried out machinery by albumen powder of getting blisters and got blisters acquisition
The fissipation factor tan δ of foam are 10-40.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300562A (en) * | 1999-12-09 | 2001-06-27 | 弗·哈夫曼-拉罗切有限公司 | Composition containing fat-soluble vitamins |
| CN105996038A (en) * | 2016-07-05 | 2016-10-12 | 南昌大学 | Method for preparing high-stability nano emulsion with controllable nutrient release |
| CN106923349A (en) * | 2017-05-09 | 2017-07-07 | 江苏康德蛋业有限公司 | A kind of preparation method of the egg albumen powder of high foaming |
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2017
- 2017-10-18 CN CN201710972252.4A patent/CN107801980B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300562A (en) * | 1999-12-09 | 2001-06-27 | 弗·哈夫曼-拉罗切有限公司 | Composition containing fat-soluble vitamins |
| CN105996038A (en) * | 2016-07-05 | 2016-10-12 | 南昌大学 | Method for preparing high-stability nano emulsion with controllable nutrient release |
| CN106923349A (en) * | 2017-05-09 | 2017-07-07 | 江苏康德蛋业有限公司 | A kind of preparation method of the egg albumen powder of high foaming |
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| Title |
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| Complex Formation of Whey Proteins: Exocellular;Fanny Weinbreck;《Langmuir》;20031231;第19卷(第22期);第9404-9410页 * |
| Influence of Phosphorylation on the Foamability and Stability of Bovine Serum Albumin and Citrus Peel Pectin Mixed Foams;Shrikant Modak等;《Journal of Dispersion Science and Technology》;20160921;第38卷(第9期);第1266-1275页 * |
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